CN108370839A - A kind of Wild ganoderma mother culture media and preparation method thereof - Google Patents
A kind of Wild ganoderma mother culture media and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a kind of Wild ganoderma mother culture medias and preparation method thereof, belong to culture medium preparing technical field.The Wild ganoderma mother culture media is Semen Coicis culture medium or sophora bud culture medium, wherein Semen Coicis culture medium includes the following raw material：80 120g of Semen Coicis, 18 22g of 40 60g of wheat bran, 20 25g of glucose and agar, sophora bud culture medium includes the following raw material：50 70g of the sophora bud, 18 22g of 40 60g of wheat bran, 20 30g of glucose and agar.The invention also discloses the preparation methods of above-mentioned Wild ganoderma mother culture media.The culture medium of the present invention is suitble to the growth of Wild ganoderma parent species, and obtained Ganoderma Lucidum stem is long, and cap is solid, and shape is close to Wild ganoderma, and institute's produce surviving of son entity goes out that sesame is neat, sesame lid rule, and meat is thick, and handle is long, and the active constituent contents such as stable yield and contained polysaccharide, triterpene are high.
Description
Technical field
The present invention relates to a kind of Wild ganoderma mother culture medias and preparation method thereof, belong to culture medium preparing technical field.
Background technology
Wild ganoderma, also known as celestial grass, seocho, polyporus lucidus belong to Basidiomycetes Polyporaceae Ganoderma, be it is a kind of with spore into
Row generative propagation, using lignin as the medicinal fungi of primary carbon source nutrient, since ancient times just by our people as Chinese herbal medicine profit
With.Ganoderma lucidum, sweet in flavor, mild-natured, the thoughts of returning home, lung, liver and kidney channel have the effect of benefiting qi and nourishing blood, mental-tranquilization, cough-relieving flat cough.
Ganoderma lucidum is containing there are many amino acid, protein, alkaloid, coumarin, steroid, triterpenes, volatile oil, mannitol, trees
Fat and carbohydrate, vitamin B2, vitamin C, lactone and enzyme.Studies have shown that ganoderma lucidum has, nourishing, brain tonic is strong, anti-inflammatory diuresis
Stomach invigorating, eliminating the phlegm, protecting liver and detoxication, adjusts blood glucose, control blood pressure, adjuvant therapy chemicotherapy, liver protecting, promotion at antitumor, analgesia
The functions such as sleep, improve the immunity of the human body, moreover it is possible to treat chronic bronchitis, cardiovascular and cerebrovascular disease, coronary heart disease, neurasthenia,
Insomnia, diabetes, migraine, cytopenia, myocarditis and impotence and seminal emission, frequency flu, eliminate skin blackspot,
Whelk.
Currently, people are increasing for the demand of ganoderma lucidum, especially Wild ganoderma.In order to meet the needs of social, open
Beginning plants ganoderma lucidum in large quantity.It has been investigated that different planted medium and cultural method are to ganoderma lucidum quality and yield
Influence it is very big.Currently, it is few for the research of Wild ganoderma mother culture media, in consideration of it, it is necessary to study a kind of wild spirits
Sesame mother culture media can solve the deficiencies in the prior art.
Invention content
An object of the present invention is to provide a kind of Wild ganoderma mother culture media.The culture medium of the present invention is suitble to wild
Lucid ganoderma stock culture is grown, and obtained Ganoderma Lucidum stem is long, and cap is solid, growth time 5-6 months, and shape is close to Wild ganoderma, institute's produce surviving of son
Entity goes out that sesame is neat, sesame lid rule, and meat is thick, and handle is long, glossy, and texture is excellent, and biological transformation ratio is high, stable yield and institute
Containing the active constituent contents such as polysaccharide, triterpene height.
The technical solution that the present invention solves above-mentioned technical problem is as follows：A kind of Wild ganoderma mother culture media is trained for Semen Coicis
Support base or sophora bud culture medium, wherein Semen Coicis culture medium includes the following raw material：Semen Coicis 80-120g, wheat bran 40-60g, glucose
20-25g and agar 18-22g, sophora bud culture medium include the following raw material：Sophora bud 50-70g, wheat bran 40-60g, glucose 20-30g
With agar 18-22g.
The Wild ganoderma culture medium of the present invention,
Wherein, Semen Coicis, alias：Job's tears, the seed of jog's tears, myotonin, ditch rice are the drying and ripening kind of grass Job's tears
Benevolence additionally contains selenium element, the heart of a lotus seed containing carbohydrate, fat oil, amino acid (for leucine, lysine, arginine, tyrosine etc.)
Job's tears element and vitamin B1.Culture medium is made using Semen Coicis as raw material in the present invention, can increase triterpene content in finished product Semen Coicis culture medium
With selenium element content.It is fast, energetic with the mycelia growth of the lucidum strain produced of Semen Coicis culture medium of the present invention, it is dense thick
Strong, vigorous, anti-miscellaneous bacteria, disease resistance are strong.The ganoderma lucidum piece shape produced is big, solid, handle is long, shape Wild ganoderma, medicinal ingredient
Triterpene, Se content be higher than other ganoderma lucidums (ganoderma lucidum for including the Wild ganoderma and artificial cultivation grown naturally).
The sophora bud, be legume Chinese scholartree (Sophora japonica L.) dry flower and flower, first recorded in《Solar corona is originally
Grass》,《Sheng Nong's herbal classic》It is middle to be classified as top grade.Rich in flavones ingredients such as rutin and Quercetins in the sophora bud, wherein main living
Property ingredient be rutin, content is up to 13-16%.Culture medium is made using the sophora bud as raw material in the present invention, can increase the training of finished product Semen Coicis
Support triterpene content in base.It is fast, energetic with the mycelia growth of the lucidum strain produced of sophora bud culture medium of the present invention, it is dense
Sturdy, vigorous, anti-miscellaneous bacteria, disease resistance is strong.The ganoderma lucidum piece shape produced is big, solid, handle is long, shape Wild ganoderma, it is medicinal at
Triterpene, the Se content divided is higher than other ganoderma lucidums (ganoderma lucidum for including the Wild ganoderma and artificial cultivation grown naturally).
Based on the above technical solution, the present invention can also be improved as follows.
Further, the Semen Coicis culture medium includes the following raw material：Semen Coicis 100g, wheat bran 50g, glucose 22g and agar
20g。
Further, the sophora bud culture medium includes the following raw material：Sophora bud 60g, wheat bran 50g, glucose 25g and agar 20g.
The second object of the present invention is to provide the preparation method of above-mentioned Wild ganoderma mother culture media.The present invention's is wild
The preparation method of lucid ganoderma stock culture culture medium is simple, low raw-material cost, and operation is easy, wide market, is suitble to scale metaplasia
Production.
The technical solution that the present invention solves above-mentioned technical problem is as follows：A kind of preparation side of Wild ganoderma mother culture media
Method, the Wild ganoderma mother culture media are Semen Coicis culture medium or sophora bud culture medium, wherein the preparation method of Semen Coicis culture medium
Include the following steps：
Step 1：80-120g Semen Coicis is taken, water 500-700mL is added, after boiling, is simmered in water 40-50 minutes, with 6 layers of gauze
Filtering, taking juice, as Semen Coicis juice；
Step 2：40-60g wheat bran is taken, water 300-500mL is added, after boiling, is simmered in water 30-40 minutes, with 8 layers of gauze mistake
Filter, taking juice, as wheat bran juice；
Step 3：After the Semen Coicis juice that step 1 obtains and the wheat bran juice that step 2 obtains are mixed, 18-22g fine jades are added
Fat, after agar melts completely, with 6 layers of filtered through gauze, taking juice as mixes juice；
Step 4：The mixing juice that step 3 is obtained adds water to add to 800-1200mL, 20-25g glucose is added, no
Disconnected stirring, after glucose is completely dissolved, it is 5-6 to get to the Semen Coicis culture medium to adjust pH value；
The preparation method of sophora bud culture medium includes the following steps：
Step 1：The 50-70g sophora bud is taken, water 700-900mL is added, after boiling, is simmered in water 50-60 minutes, with 6 layers of gauze mistake
Filter, taking juice, as sophora bud juice；
Step 2：40-60g wheat bran is taken, water 300-500mL is added, after boiling, is simmered in water 30-40 minutes, with 8 layers of gauze mistake
Filter, taking juice, as wheat bran juice；
Step 3：After the sophora bud juice that step 1 obtains and the wheat bran juice that step 2 obtains are mixed, 18-22g fine jades are added
Fat, after agar melts completely, with 6 layers of filtered through gauze, taking juice as mixes juice；
Step 4：The mixing juice that step 3 is obtained adds water to add to 800-1200mL, 20-30g glucose is added, no
Disconnected stirring, after glucose is completely dissolved, it is 5-6 to get to the sophora bud culture medium to adjust pH value.
Based on the above technical solution, the present invention can also be improved as follows.
Further, the preparation method of the Semen Coicis culture medium includes the following steps：
Step 1：100g Semen Coicis is taken, water 600mL is added, after boiling, simmers in water 45 minutes, with 6 layers of filtered through gauze, takes juice
Liquid, as Semen Coicis juice；
Step 2：50g wheat bran is taken, water 400mL is added, after boiling, is simmered in water 35 minutes, with 8 layers of filtered through gauze, taking juice,
As wheat bran juice；
Step 3：After the Semen Coicis juice that step 1 obtains and the wheat bran juice that step 2 obtains are mixed, 20g agar is added, waits for
After agar melts completely, with 6 layers of filtered through gauze, taking juice as mixes juice；
Step 4：The mixing juice that step 3 is obtained adds water to add to 1000mL, and 22g glucose is added, is stirred continuously,
After glucose is completely dissolved, it is 5-6 to get to the Semen Coicis culture medium to adjust pH value.
Further, the preparation method of the sophora bud culture medium includes the following steps：
Step 1：The 60g sophora bud is taken, water 800mL is added, after boiling, is simmered in water 55 minutes, with 6 layers of filtered through gauze, taking juice,
As sophora bud juice；
Step 2：50g wheat bran is taken, water 400mL is added, after boiling, is simmered in water 35 minutes, with 8 layers of filtered through gauze, taking juice,
As wheat bran juice；
Step 3：After the sophora bud juice that step 1 obtains and the wheat bran juice that step 2 obtains are mixed, 20g agar is added, waits for
After agar melts completely, with 6 layers of filtered through gauze, taking juice as mixes juice；
Step 4：The mixing juice that step 3 is obtained adds water to add to 1000mL, and 25g glucose is added, is stirred continuously,
After glucose is completely dissolved, it is 5-6 to get to the sophora bud culture medium to adjust pH value.
Beneficial effects of the present invention：
(1) Wild ganoderma mother culture media of the invention is suitble to Wild ganoderma parent species growth, and obtained Ganoderma Lucidum stem is long, bacterium
Cover solid, growth time 5-6 months, shape was close to Wild ganoderma, and institute's produce surviving of son entity goes out that sesame is neat, sesame lid rule, and meat is thick, handle
Long, glossy, texture is excellent, and biological transformation ratio height, stable yield and the active constituent contents such as contained polysaccharide, triterpene are high.
(2) preparation method of Wild ganoderma mother culture media of the invention is simple, low raw-material cost, and operation is easy, city
Field has a extensive future, and is suitble to large-scale production.
Specific implementation mode
Principles and features of the present invention are described below in conjunction with specific embodiment, example is served only for explaining this hair
It is bright, it is not intended to limit the scope of the present invention.
Embodiment 1：
The Wild ganoderma mother culture media of the present embodiment is Semen Coicis culture medium, including the following raw material：Semen Coicis 80g, wheat bran
40g, glucose 20g and agar 18g.
The preparation method of above-mentioned Semen Coicis culture medium includes the following steps：
Step 1：80g Semen Coicis is taken, water 500mL is added, after boiling, is simmered in water 40 minutes, with 6 layers of filtered through gauze, taking juice,
As Semen Coicis juice；
Step 2：40g wheat bran is taken, water 300mL is added, after boiling, is simmered in water 30 minutes, with 8 layers of filtered through gauze, taking juice,
As wheat bran juice；
Step 3：After the Semen Coicis juice that step 1 obtains and the wheat bran juice that step 2 obtains are mixed, 18g agar is added, waits for
After agar melts completely, with 6 layers of filtered through gauze, taking juice as mixes juice；
Step 4：The mixing juice that step 3 is obtained adds water to add to 800mL, and 20g glucose is added, is stirred continuously, waits for
After glucose is completely dissolved, it is 5-6 to get to the Semen Coicis culture medium to adjust pH value.
Embodiment 2：
The Wild ganoderma mother culture media of the present embodiment is Semen Coicis culture medium, including the following raw material：Semen Coicis 100g, wheat bran
50g, glucose 22g and agar 20g.
The preparation method of above-mentioned Semen Coicis culture medium includes the following steps：
Step 1：100g Semen Coicis is taken, water 600mL is added, after boiling, simmers in water 45 minutes, with 6 layers of filtered through gauze, takes juice
Liquid, as Semen Coicis juice；
Step 2：50g wheat bran is taken, water 400mL is added, after boiling, is simmered in water 35 minutes, with 8 layers of filtered through gauze, taking juice,
As wheat bran juice；
Step 3：After the Semen Coicis juice that step 1 obtains and the wheat bran juice that step 2 obtains are mixed, 20g agar is added, waits for
After agar melts completely, with 6 layers of filtered through gauze, taking juice as mixes juice；
Step 4：The mixing juice that step 3 is obtained adds water to add to 1000mL, and 22g glucose is added, is stirred continuously,
After glucose is completely dissolved, it is 5-6 to get to the Semen Coicis culture medium to adjust pH value.
Embodiment 3：
The Wild ganoderma mother culture media of the present embodiment is sophora bud culture medium, is made of the following raw material：Sophora bud 60g, wheat bran
50g, glucose 25g and agar 20g.
The preparation method of above-mentioned sophora bud culture medium includes the following steps：
Step 1：The 60g sophora bud is taken, water 800mL is added, after boiling, is simmered in water 55 minutes, with 6 layers of filtered through gauze, taking juice,
As sophora bud juice；
Step 2：50g wheat bran is taken, water 400mL is added, after boiling, is simmered in water 35 minutes, with 8 layers of filtered through gauze, taking juice,
As wheat bran juice；
Step 3：After the sophora bud juice that step 1 obtains and the wheat bran juice that step 2 obtains are mixed, 20g agar is added, waits for
After agar melts completely, with 6 layers of filtered through gauze, taking juice as mixes juice；
Step 4：The mixing juice that step 3 is obtained adds water to add to 1000mL, and 25g glucose is added, is stirred continuously,
After glucose is completely dissolved, it is 5-6 to get to the sophora bud culture medium to adjust pH value
Comparative example 1
As different from Example 1, comparative example 1 replaces Semen Coicis with potato, remaining is all identical, i.e. the culture of comparative example 1
Base includes the raw material of following weight：Potato 80g, wheat bran 40g, glucose 20g and agar 18g.
The preparation method of the culture medium of comparative example 1, with embodiment 1.
Wild ganoderma parent species are cultivated in the culture medium that comparative example 1 and embodiment 1 obtain respectively, keeps growing environment
Unanimously, i.e., it is grown on 1000 meters of height above sea level or more.Wherein, Wild ganoderma parent species are purchased from Guilin Guanyang woods and fill bacterium industry scientific and technological development
Co., Ltd passes through for the acquisition wild Ganoderma Sinense of Guilin Haiyang Shan Mountain (the long 17cm of a diameter of 29cm, handle, semicircle, surface puce)
Selection and breeding are best in quality, the strong high-quality lucidum strain of anti-miscellaneous bacteria.
The yield and quality of the Wild ganoderma of comparative example 1 and embodiment 1 is measured respectively, it is specific as shown in table 1.
The cultivation of glossy ganoderma quality versus of table 1 comparative example 1 and embodiment 1
Comparative example 2
As different from Example 2, comparative example 2 replaces Semen Coicis with weed tree sawdust, remaining is all identical, i.e. the culture of comparative example 2
Base includes the raw material of following weight：Weed tree sawdust 100g, wheat bran 50g, glucose 22g and agar 20g.
The preparation method of the culture medium of comparative example 2, with embodiment 2.
Wild ganoderma parent species are acquired, are cultivated respectively in the culture medium that comparative example 2 and embodiment 2 obtain, growth ring is kept
Border is consistent, i.e., is grown on 1000 meters of height above sea level or more, measures the yield and matter of the Wild ganoderma of comparative example 2 and embodiment 2 respectively
Amount, it is specific as shown in table 2.
The cultivation of glossy ganoderma quality versus of table 2 comparative example 2 and embodiment 2
Comparative example 3
As different from Example 3, comparative example 3 replaces the sophora bud with weed tree sawdust, remaining is all identical, i.e. the culture of comparative example 3
Base includes the raw material of following weight：Weed tree sawdust 60g, wheat bran 50g, glucose 25g and agar 20g.
The preparation method of the culture medium of comparative example 3, with embodiment 3.
Wild ganoderma parent species are acquired, are cultivated respectively in the culture medium that comparative example 3 and embodiment 3 obtain, growth ring is kept
Border is consistent, i.e., is grown on 1000 meters of height above sea level or more, measures the yield and matter of the Wild ganoderma of comparative example 3 and embodiment 3 respectively
Amount, it is specific as shown in table 3.
The cultivation of glossy ganoderma quality versus of table 3 comparative example 3 and embodiment 3
It can be seen that the Wild ganoderma mother culture media of the present invention, is suitble to Wild ganoderma parent species growth, obtained Ganoderma Lucidum stem
Long, cap is solid, growth time 5-6 months, and shape is close to Wild ganoderma, and institute's produce surviving of son entity goes out that sesame is neat, sesame lid rule, meat
Thickness, handle is long, glossy, and texture is excellent, biological transformation ratio height, stable yield and the active constituent contents such as contained polysaccharide, triterpene
It is high.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.
Claims (6)
1. a kind of Wild ganoderma mother culture media, which is characterized in that be Semen Coicis culture medium or sophora bud culture medium, wherein Semen Coicis
Culture medium includes the following raw material：Semen Coicis 80-120g, wheat bran 40-60g, glucose 20-25g and agar 18-22g, sophora bud culture medium
Including the following raw material：Sophora bud 50-70g, wheat bran 40-60g, glucose 20-30g and agar 18-22g.
2. a kind of Wild ganoderma mother culture media according to claim 1, which is characterized in that the Semen Coicis culture medium includes
The following raw material：Semen Coicis 100g, wheat bran 50g, glucose 22g and agar 20g.
3. a kind of Wild ganoderma mother culture media according to claim 1, which is characterized in that the sophora bud culture medium includes
The following raw material：Sophora bud 60g, wheat bran 50g, glucose 25g and agar 20g.
4. a kind of preparation method of Wild ganoderma mother culture media, which is characterized in that the Wild ganoderma mother culture media is the heart of a lotus seed
Rice culture medium or sophora bud culture medium, wherein the preparation method of Semen Coicis culture medium includes the following steps：
Step 1：80-120g Semen Coicis is taken, water 500-700mL is added, after boiling, is simmered in water 40-50 minutes, with 6 layers of filtered through gauze,
Taking juice, as Semen Coicis juice；
Step 2：40-60g wheat bran is taken, water 300-500mL is added, after boiling, is simmered in water 30-40 minutes, with 8 layers of filtered through gauze,
Taking juice, as wheat bran juice；
Step 3：After the Semen Coicis juice that step 1 obtains and the wheat bran juice that step 2 obtains are mixed, 18-22g agar is added, waits for
After agar melts completely, with 6 layers of filtered through gauze, taking juice as mixes juice；
Step 4：The mixing juice that step 3 is obtained adds water to add to 800-1200mL, and 20-25g glucose is added, constantly stirs
It mixes, after glucose is completely dissolved, it is 5-6 to get to the Semen Coicis culture medium to adjust pH value；
The preparation method of sophora bud culture medium includes the following steps：
Step 1：The 50-70g sophora bud is taken, water 700-900mL is added, after boiling, is simmered in water 50-60 minutes, with 6 layers of filtered through gauze,
Taking juice, as sophora bud juice；
Step 2：40-60g wheat bran is taken, water 300-500mL is added, after boiling, is simmered in water 30-40 minutes, with 8 layers of filtered through gauze,
Taking juice, as wheat bran juice；
Step 3：After the sophora bud juice that step 1 obtains and the wheat bran juice that step 2 obtains are mixed, 18-22g agar is added, waits for
After agar melts completely, with 6 layers of filtered through gauze, taking juice as mixes juice；
Step 4：The mixing juice that step 3 is obtained adds water to add to 800-1200mL, and 20-30g glucose is added, constantly stirs
It mixes, after glucose is completely dissolved, it is 5-6 to get to the sophora bud culture medium to adjust pH value.
5. a kind of preparation method of Wild ganoderma mother culture media according to claim 4, which is characterized in that the Semen Coicis training
The preparation method for supporting base includes the following steps：
Step 1：100g Semen Coicis is taken, water 600mL is added, after boiling, is simmered in water 45 minutes, with 6 layers of filtered through gauze, taking juice, i.e.,
For Semen Coicis juice；
Step 2：50g wheat bran is taken, water 400mL is added, after boiling, is simmered in water 35 minutes, with 8 layers of filtered through gauze, taking juice, as
Wheat bran juice；
Step 3：After the Semen Coicis juice that step 1 obtains and the wheat bran juice that step 2 obtains are mixed, 20g agar is added, waits for agar
After melting completely, with 6 layers of filtered through gauze, taking juice as mixes juice；
Step 4：The mixing juice that step 3 is obtained adds water to add to 1000mL, and 22g glucose is added, is stirred continuously, waits for Portugal
After grape sugar is completely dissolved, it is 5-6 to get to the Semen Coicis culture medium to adjust pH value.
6. a kind of preparation method of Wild ganoderma mother culture media according to claim 4, which is characterized in that the sophora bud training
The preparation method for supporting base includes the following steps：
Step 1：The 60g sophora bud is taken, water 800mL is added, after boiling, is simmered in water 55 minutes, with 6 layers of filtered through gauze, taking juice, as
Sophora bud juice；
Step 2：50g wheat bran is taken, water 400mL is added, after boiling, is simmered in water 35 minutes, with 8 layers of filtered through gauze, taking juice, as
Wheat bran juice；
Step 3：After the sophora bud juice that step 1 obtains and the wheat bran juice that step 2 obtains are mixed, 20g agar is added, waits for agar
After melting completely, with 6 layers of filtered through gauze, taking juice as mixes juice；
Step 4：The mixing juice that step 3 is obtained adds water to add to 1000mL, and 25g glucose is added, is stirred continuously, waits for Portugal
After grape sugar is completely dissolved, it is 5-6 to get to the sophora bud culture medium to adjust pH value.
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| CN106613301A (en) * | 2015-10-29 | 2017-05-10 | 开县金地农业开发有限公司 | Cultivating and planting method of selenium-enriched ganodorma lucidum |
| CN107548893A (en) * | 2016-06-29 | 2018-01-09 | 黄秀英 | Ganoderma lucidum bion drink is cultivated by carrier of Semen Coicis |
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- 2018-01-25 CN CN201810073188.0A patent/CN108370839A/en active Pending
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| CN1810082A (en) * | 2006-02-24 | 2006-08-02 | 崔澎 | Method of cultivating selenium-rich pagodatree ear and method of extracting pagodatree ear selenium polysaccharide |
| CN101100644A (en) * | 2007-06-19 | 2008-01-09 | 林长勇 | Method for culturing and processing edible fungus active function hypha powder |
| CN103229669A (en) * | 2013-05-14 | 2013-08-07 | 四川省砚山中药饮片有限公司 | Mushroom cultivating method utilizing residues of sophora flower buds after extraction of rutins |
| CN104956914A (en) * | 2015-06-18 | 2015-10-07 | 唐伟 | Breeding method for natural ganoderma lucidum |
| CN106613301A (en) * | 2015-10-29 | 2017-05-10 | 开县金地农业开发有限公司 | Cultivating and planting method of selenium-enriched ganodorma lucidum |
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Application publication date: 20180807 |