CN112189505A - Artificially-cultured phellinus igniarius sporophore extract for treating gout and preparation method thereof - Google Patents
Artificially-cultured phellinus igniarius sporophore extract for treating gout and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
Abstract
The invention belongs to the technical field of artificial cultivation of edible fungi, and particularly relates to an artificially-cultivated phellinus igniarius sporocarp extract for treating gout, and further discloses a preparation method of the extract. According to the artificially cultured phellinus igniarius sporocarp extract for treating gout, the selected phellinus igniarius sporocarp raw materials are efficiently cultured by adopting artificial bag materials, the phellinus igniarius sporocarp is cultured on the basis of the traditional artificial bag material type culture method, and the phellinus igniarius sporocarp is extracted by using ethanol as the raw material, so that the extract has a good effect of reducing uric acid and can be used for treating gout.
Description
Technical Field
The invention belongs to the technical field of artificial cultivation of edible fungi, and particularly relates to an artificially-cultivated phellinus igniarius sporocarp extract for treating gout, and further discloses a preparation method of the extract.
Background
Phellinus igniarius (Phellinus igniarius) belongs to Basidiomycotina (Basidiomycotina), Hymenomycetes (Hymenomycetes), Aphyllophorales (Aphyllophorales), Hymenochaeyaceae (Hymenochaeyaceae), Phellinus (Phellinus), and is the trade name of Phellinus igniarius (P.igniarius), Phellinus baumii (P.baumii) and Phellinus linteus (P.Linteus), and is generally grown on the stems of withered trees and stumps of broad-leaved trees such as poplar, mulberry, willow, white birch, zelkova, peach, and the like. Phellinus linteus is a precious medicinal fungus developed in recent years for many years and is named as forest gold in the name of America. According to the records of Chinese medicine dictionary, Phellinus can be used for treating gynecological diseases such as metrorrhagia, bloody stranguria, leukorrhagia, amenorrhea, etc.; modern medical research also shows that phellinus igniarius has remarkable effects of resisting tumor, bacteria and fibrosis, resisting oxidation, improving human immunity and the like, is the rare medicinal fungus with the first biological anti-tumor effect internationally acknowledged at present, and is widely applied to the industries of medicines, foods, daily chemicals, health care products and the like.
At present, due to the restriction of the particularity and complexity of physiological states and external environment, the formation of the fruit body of phellinus igniarius in nature is difficult, so that the natural phellinus igniarius is rare in quantity, the resource available for medicine in nature is limited, and the demand of domestic and foreign markets for phellinus igniarius is increased, so that medicine farmers in domestic places take away to collect the phellinus igniarius, the fruit body cannot be formed in large quantity, the phellinus igniarius cannot become a stable industrial product source, and the development of the phellinus igniarius industry is very unfavorable, so that the defect of wild resources is made up through artificial cultivation, and the method is very important. However, artificial cultivation of Phellinus linteus is extremely difficult, and has problems of harsh culture conditions and long growth cycle.
At present, two methods are mainly used for artificially cultivating phellinus igniarius, one of the methods is mainly the cut-log cultivation of mulberry trees, poplar trees and oak trees, but the cut-log cultivation needs a large amount of wood resources, the resource waste is serious, and the large-scale cultivation is difficult; the other is bag cultivation, but has the problems of insufficient nutrient supply of the culture medium, small fruit body development, easy infectious microbe infection and the like.
For example, in the artificial high-yield cultivation method of phellinus igniarius disclosed in chinese patent CN1720785A, the scheme is to adopt bag cultivation, in order to solve the problems of insufficient nutrient supply of the culture medium, small development of fruit body, easy contamination of infectious microbes and the like, auxin is added into the culture medium, however, the problem of the culture medium is not fundamentally solved by the addition of the auxin, and the addition of the auxin has influence on the quality of phellinus igniarius and is not beneficial to the natural growth of phellinus igniarius. Also, like the culture medium for cultivating phellinus igniarius bag material disclosed in chinese patent CN102786333A and the method for cultivating phellinus igniarius sporocarp by using the culture medium, the method makes full use of resources such as ramulus mori, mulberry leaves and the like, can effectively reduce the waste of log resources, can quickly cultivate phellinus igniarius sporocarp in large scale, is similar to wild phellinus igniarius, and has stable quality; however, the problem of long culture time still remains.
Gout belongs to the category of metabolic diseases, and is a series of inflammatory reactions caused by purine substance metabolism disorder in organisms or reduction of uric acid excretion of kidney, so that urate deposition is caused. When uric acid in serum is increased to a supersaturated state, extracellular fluid is deposited on joints, tissues and organs around the joints, so that a group of gout syndromes (including tophus, uric acid kidney stones, gouty arthritis and gouty nephropathy) are caused. According to statistics, the total incidence of gout is 0.06-2.68 per mill. Gout has become the second disease of diabetes in China, and the onset age of gout is in a tendency of being younger. At present, gout treatment approaches mainly reduce the incidence of hyperuricemia by inhibiting excessive uric acid production and promoting uric acid excretion, and inhibit inflammatory cytokines and the like. Although the chemical medicine for clinically treating gout has quick response, the adverse reactions are more, such as febuxostat, benzbromarone, colchicine and the like have toxic and side effects of different degrees, including kidney injury, central nervous system toxicity and the like, and the occurrence probability is high. In the prior art, a treatment scheme for treating gout diseases based on phellinus igniarius extract is developed, but the treatment scheme is limited by the problems of small wild phellinus igniarius amount, unstable quality of artificial phellinus igniarius and the like, so that the development of subsequent medicines is influenced.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to provide an artificially cultured phellinus igniarius sporocarp extract for treating gout, wherein the extract has higher effective components and better treatment effect;
the second technical problem to be solved by the present invention is to provide a method for preparing the above-mentioned artificially cultivated phellinus linteus fruit body extract for treating gout.
In order to solve the technical problems, the preparation method of the artificially cultured phellinus igniarius sporocarp extract for treating gout comprises the steps of artificially culturing the phellinus igniarius sporocarp and extracting the phellinus igniarius sporocarp, and specifically comprises the following steps of:
(1) preparing a cultivation bag material: taking 20-30 parts by weight of mulberry twig sawdust, 30-50 parts by weight of brewing waste residue, 10-30 parts by weight of molasses, 3-8 parts by weight of activated sludge particles, 1-5 parts by weight of quick lime and KH2PO40.2-0.5 weight part, and uniformly mixing to obtain the required culture medium; adding water into the culture medium, mixing uniformly, and bagging for later use;
(2) mycelium culture: inoculating a phellinus igniarius mother seed into the cultivation bag material, and carrying out constant-temperature cultivation on the inoculated cultivation bag material until the cultivation bag material is full of mycelia;
(3) and (3) fruiting body culture: cutting the bag material full of mycelia to produce mushrooms, continuously carrying out constant-temperature culture until the sporocarp is mature, and harvesting;
(4) pulverizing the phellinus igniarius sporocarp to obtain powder, adding an ethanol water solution for soaking and reflux extraction, collecting an extracting solution, concentrating under reduced pressure until no ethanol smell exists, and drying in vacuum to obtain the phellinus igniarius sporocarp extract required for artificial cultivation.
Specifically, in the step (1), the activated sludge particles are aerobic activated sludge particles.
Specifically, in the step (1), the mass ratio of the culture medium to water is 40-50%: 50 to 60 percent.
Specifically, in the step (1), the culture medium further comprises a step of adding an edible fungus extract as a functional activity inducer.
Specifically, the edible fungus extract comprises an agrocybe aegerita extract, preferably an agrocybe aegerita ethanol extract, and preferably the addition amount of the agrocybe aegerita extract is 3-8 parts by weight.
Specifically, in the step (2), the inoculation amount of the phellinus igniarius mother seeds is 5-10 wt% of the culture medium.
Specifically, in the step (2), the process conditions of the mycelium culturing step include: the culture temperature is controlled to be 25-30 ℃, the air humidity is 50-80%, and the illumination intensity is 10-20 lux.
Specifically, in the step (3), the process conditions of the fruiting body cultivation step include: the culture temperature is controlled to be 25-30 ℃, the air humidity is 70-80%, and the illumination intensity is 30-100 lux.
Specifically, in the step (4):
the volume percentage content of the ethanol water solution is 60-80 v/v%;
the dosage ratio of the phellinus igniarius sporophore powder to the ethanol water solution is 10 g: 200 and 300 mL.
The invention also discloses an artificially cultured phellinus igniarius sporocarp extract for treating gout, which is extracted by the method.
The artificially cultured phellinus igniarius sporocarp extract for treating gout is obtained by extracting phellinus igniarius sporocarp serving as a raw material with ethanol, and the obtained extract has a good uric acid reducing effect and can be used for treating gout. According to the method, the phellinus igniarius sporocarp raw material selected by the extract is efficiently cultivated by adopting artificial bag materials, the cultivation of the phellinus igniarius sporocarp is tried based on the traditional artificial bag material type cultivation method, mulberry twig wood chips, brewing waste residues and molasses are selected as main cultivation raw materials, and the recycling of industrial waste materials such as the brewing waste residues and the molasses is realized under the condition that the nutrition components of the phellinus igniarius cultivation are met; the activated sludge particles further added depend on the components of microorganisms, organic matters and the like contained in the activated sludge particles, so that the absorption of nutrient components of the cultivation raw materials and the forming of phellinus igniarius mycelia and sporocarp are greatly promoted, the cultivation period of phellinus igniarius is greatly shortened, the cultivation efficiency of phellinus igniarius sporocarp is effectively improved, and the yield and quality guarantee is provided for the downstream application of phellinus igniarius.
The phellinus igniarius sporocarp raw material selected by the extract is further added with the agrocybe cylindracea extract to carry out induced deposition of effective components in the process of cultivating the phellinus igniarius sporocarp, so that the obtained phellinus igniarius sporocarp extract has a better effect of reducing uric acid, and the phellinus igniarius sporocarp is presumed to be caused by efficient accumulation of the effective components for reducing uric acid in the phellinus igniarius sporocarp, so that the phellinus igniarius sporocarp has a better medicinal value.
Detailed Description
In the following examples of the present invention, the phellinus linteus mother strain is obtained by separation and purification by a conventional method, activated by a conventional PDA plate medium, and inoculated into a conventional PD mother strain medium for culture, thereby obtaining the phellinus linteus mother strain.
In the following examples of the present invention, the aerobic activated sludge granules are commercially available conventional products in the prior art.
In the following embodiments of the present invention, the brewing waste residue is waste residue generated in a general liquor brewing process, such as waste residue obtained in a general kaoliang spirit brewing process, for example, the method includes the following steps:
(1) taking 90kg of sorghum, crushing the sorghum into 4-8 pieces/granule of the sorghum, uniformly mixing, adding 55kg of water (75 ℃) for grain moistening for 18 hours, and then filling into a steamer and steaming for 80min to obtain grain grains;
(2) taking the grain stillage out of the retort in the step S1, adding 28kg of cold water for raising the cold, adding 10kg of Daqu and 0.03kg of aroma-producing active dry yeast after raising the cold to obtain large residues, and putting the large residues into a pool at 12 ℃ for large residue fermentation for 28 days;
(3) taking the fermented large residues, adding 15kg of rice hulls, uniformly mixing, filling into a steamer, and distilling for 50min to obtain large residue wine and grain lees for later use;
(4) taking the grain stillage obtained in the step S3 out of a steamer, adding 30kg of water, cooling, adding 8kg of Daqu and 0.02kg of aroma-producing active dry yeast to obtain secondary slag, and putting the secondary slag into a jar at 15 ℃ for secondary slag fermentation for 28 days;
(5) taking the fermented second residue, adding rice hulls accounting for 10 wt% of the weight of the second residue, uniformly mixing, filling into a steamer, and distilling for 50min to obtain second residue wine;
(6) blending the obtained big dreg wine and the second dreg wine according to the mass ratio of 45:55, mixing and blending the two dregs wine, and then reducing the mixture to 54 ℃ to obtain the wine.
And mixing the waste residues generated by the solid-liquid separation in the steps to obtain the brewing waste residues of the common brewing process, and carrying out solid-liquid separation and drying treatment on the brewing waste residues until the water content is lower than 10%.
In the following embodiments of the present invention, the edible fungus extract is an agrocybe aegerita ethanol extract, and the specific steps include: crushing the fruiting body of agrocybe cylindracea, adding 3 times of ethanol water solution with volume concentration of 75 v/v% for reflux extraction at normal temperature for 3 hours, collecting the extracting solution, concentrating under reduced pressure until no ethanol smell exists, and drying in vacuum to obtain the required agrocybe cylindracea extract.
Example 1
The preparation method of the artificially cultured phellinus igniarius sporocarp extract for treating gout comprises the following steps of:
(1) preparing a cultivation bag material: taking 20 parts by weight of mulberry twig sawdust, 50 parts by weight of wine-making waste residue, 10 parts by weight of molasses, 8 parts by weight of aerobic activated sludge particles, 1 part by weight of quicklime and KH2PO40.5 part by weight, and uniformly mixing to obtain the required culture medium; according to the proportion of 50%: adding water into the culture medium in a mass ratio of 50%, uniformly mixing, and bagging for later use;
(2) mycelium culture: inoculating a phellinus igniarius mother seed liquid material accounting for 10 wt% of the culture medium into the culture bag material, placing the inoculated culture bag material in a culture space with the temperature of 26-27 ℃ and the air humidity of 60-65% for constant-temperature culture, controlling the illumination condition of a culture room to be 15lux in the period, and keeping the interval ventilation state of the culture room; observing the culture condition of the bag material at any time, and recording the culture time of the mycelium overgrowing with the culture bag material;
(3) and (3) fruiting body culture: cutting the bag material full of mycelia to grow mushrooms, continuously controlling the temperature to be 27-28 ℃, the air humidity to be 70-75% and the illumination intensity to be 50lux to carry out constant-temperature culture, keeping the interval ventilation state among the cultures, observing the fruiting condition of the bag material at any time, harvesting in time and recording the mature time of the fruiting bodies;
(4) crushing the collected phellinus igniarius sporocarp into medium powder in a swing type crusher and fully and uniformly mixing; and placing another 20g of powder into a round-bottom flask, adding 500mL of ethanol water solution with volume content of 70 v/v%, soaking for 1h, performing reflux extraction for 2h, filtering, collecting filtrate, washing filter residue with 70 v/v% ethanol, combining the filtrates, placing into a rotary evaporator, recovering ethanol until no alcohol smell exists, and performing vacuum drying to obtain the required phellinus igniarius sporocarp extract.
Example 2
The preparation method of the artificially cultured phellinus igniarius sporocarp extract for treating gout comprises the following steps of:
(1) preparing a cultivation bag material: 30 parts of mulberry twig sawdust, 30 parts of brewing waste residue, 30 parts of molasses, 3 parts of aerobic activated sludge particles, 5 parts of quicklime and KH2PO40.2 part by weight, and uniformly mixing to obtain the required culture medium; according to the proportion of 50%: adding water into the culture medium in a mass ratio of 50%, uniformly mixing, and bagging for later use;
(2) mycelium culture: inoculating a phellinus igniarius mother seed liquid material accounting for 10 wt% of the culture medium into the culture bag material, placing the inoculated culture bag material in a culture space with the temperature of 26-27 ℃ and the air humidity of 60-65% for constant-temperature culture, controlling the illumination condition of a culture room to be 15lux in the period, and keeping the interval ventilation state of the culture room; observing the culture condition of the bag material at any time, and recording the culture time of the mycelium overgrowing with the culture bag material;
(3) and (3) fruiting body culture: cutting the bag material full of mycelia to grow mushrooms, continuously controlling the temperature to be 27-28 ℃, the air humidity to be 70-75% and the illumination intensity to be 50lux to carry out constant-temperature culture, keeping the interval ventilation state among the cultures, observing the fruiting condition of the bag material at any time, harvesting in time and recording the mature time of the fruiting bodies;
(4) crushing the collected phellinus igniarius sporocarp into medium powder in a swing type crusher and fully and uniformly mixing; and placing another 20g of powder into a round-bottom flask, adding 500mL of ethanol water solution with volume content of 70 v/v%, soaking for 1h, performing reflux extraction for 2h, filtering, collecting filtrate, washing filter residue with 70 v/v% ethanol, combining the filtrates, placing into a rotary evaporator, recovering ethanol until no alcohol smell exists, and performing vacuum drying to obtain the required phellinus igniarius sporocarp extract.
Example 3
The preparation method of the artificially cultured phellinus igniarius sporocarp extract for treating gout comprises the following steps of:
(1) preparing a cultivation bag material: taking mulberry twig wood chips25 parts by weight, 40 parts by weight of brewing waste residues, 20 parts by weight of molasses, 5 parts by weight of activated sludge particles, 3 parts by weight of quick lime and KH2PO40.3 part by weight, and uniformly mixing to obtain the required culture medium; according to the proportion of 50%: adding water into the culture medium in a mass ratio of 50%, uniformly mixing, and bagging for later use;
(2) mycelium culture: inoculating a phellinus igniarius mother seed liquid material accounting for 10 wt% of the culture medium into the culture bag material, placing the inoculated culture bag material in a culture space with the temperature of 26-27 ℃ and the air humidity of 60-65% for constant-temperature culture, controlling the illumination condition of a culture room to be 15lux in the period, and keeping the interval ventilation state of the culture room; observing the culture condition of the bag material at any time, and recording the culture time of the mycelium overgrowing with the culture bag material;
(3) and (3) fruiting body culture: cutting the bag material full of mycelia to grow mushrooms, continuously controlling the temperature to be 27-28 ℃, the air humidity to be 70-75% and the illumination intensity to be 50lux to carry out constant-temperature culture, keeping the interval ventilation state among the cultures, observing the fruiting condition of the bag material at any time, harvesting in time and recording the mature time of the fruiting bodies;
(4) crushing the collected phellinus igniarius sporocarp into medium powder in a swing type crusher and fully and uniformly mixing; and placing another 20g of powder into a round-bottom flask, adding 500mL of ethanol water solution with volume content of 70 v/v%, soaking for 1h, performing reflux extraction for 2h, filtering, collecting filtrate, washing filter residue with 70 v/v% ethanol, combining the filtrates, placing into a rotary evaporator, recovering ethanol until no alcohol smell exists, and performing vacuum drying to obtain the required phellinus igniarius sporocarp extract.
Example 4
The preparation method of the artificially cultured phellinus igniarius sporocarp extract for treating gout comprises the following steps of:
(1) preparing a cultivation bag material: 25 parts of mulberry twig sawdust, 40 parts of brewing waste residue, 20 parts of molasses, 5 parts of activated sludge particles, 3 parts of quick lime and KH2PO40.3 part by weight of agrocybe cylindracea extract and 5 parts by weight of agrocybe cylindracea extract are uniformly mixed to obtain the required culture medium; according to the proportion of 50%: taking the culture medium according to the mass ratio of 50%, adding water, mixing uniformly, bagging and preparingUsing;
(2) mycelium culture: inoculating a phellinus igniarius mother seed liquid material accounting for 10 wt% of the culture medium into the culture bag material, placing the inoculated culture bag material in a culture space with the temperature of 26-27 ℃ and the air humidity of 60-65% for constant-temperature culture, controlling the illumination condition of a culture room to be 15lux in the period, and keeping the interval ventilation state of the culture room; observing the culture condition of the bag material at any time, and recording the culture time of the mycelium overgrowing with the culture bag material;
(3) and (3) fruiting body culture: cutting the bag material full of mycelia to grow mushrooms, continuously controlling the temperature to be 27-28 ℃, the air humidity to be 70-75% and the illumination intensity to be 50lux to carry out constant-temperature culture, keeping the interval ventilation state among the cultures, observing the fruiting condition of the bag material at any time, harvesting in time and recording the mature time of the fruiting bodies;
(4) crushing the collected phellinus igniarius sporocarp into medium powder in a swing type crusher and fully and uniformly mixing; and placing another 20g of powder into a round-bottom flask, adding 500mL of ethanol water solution with volume content of 70 v/v%, soaking for 1h, performing reflux extraction for 2h, filtering, collecting filtrate, washing filter residue with 70 v/v% ethanol, combining the filtrates, placing into a rotary evaporator, recovering ethanol until no alcohol smell exists, and performing vacuum drying to obtain the required phellinus igniarius sporocarp extract.
Comparative example 1
The method for preparing the artificially cultured phellinus linteus fruiting body extract of the present comparative example is the same as example 3, except that the aerobic activated sludge granules are not added to the culture substrate.
Examples of the experiments
1. Phellinus igniarius sporophore character
The time for culturing mycelium and fruiting body of Phellinus linteus in the above examples 1-4 and comparative example 1 was recorded, respectively, and the weight and quality of harvested Phellinus linteus fruiting body were measured, and the recorded results are shown in Table 1 below.
TABLE 1 Phellinus linteus fruiting body culture
As can be seen from the above results, the method for cultivating phellinus igniarius sporocarp based on artificial bag material of the invention can effectively shorten the cultivation time of phellinus igniarius sporocarp and improve the cultivation quality of phellinus igniarius sporocarp.
2. Uric acid lowering effect test
60 male SPF Kunming mice (20 ± 2g) were taken and randomly divided into 6 groups: a normal control group, a hyperuricemia model control group, a positive control group, a test 1 group, a test 2 group and a control test group.
Except normal control group intraperitoneal injection and gavage normal saline, other groups intraperitoneal injection of oteracil potassium salt is carried out according to the dose of 100mg/kg/d, and meanwhile, the 600mg/kg/d dose of hypoxanthine is used for molding. Fasting for one hour before the molding without water supply, and after 1 hour after the molding, performing intragastric administration on allopurinol with the dose of 5mg/kg/d by a positive control group; test 1 group intragastric at a dose of 100mg/kg/d the extract prepared in example 3; test 2 groups of intragastric 100mg/kg/d dose of the extract prepared in example 4; the extract prepared in the comparative example 1 is used for the control test group at the intragastric administration dosage of 100 mg/kg/d; normal control group and hyperuricemia model control group were perfused with the same volume of physiological saline for 7 consecutive days.
After the administration for 1 hour by gavage on the 7 th day, the eyeballs are anaesthetized and blood is taken out, the blood serum is obtained by centrifuging for 10min at the speed of 3500r/min by using a centrifuge, and the concentration of uric acid in the blood serum is detected, and the result is shown in the table 2.
TABLE 2 results of uric acid lowering effect test of Phellinus linteus fruiting body extract
| Numbering | Serum uric acid concentration (mu mol/L) |
| Normal control group | 110 |
| High uric acidControl group of blood model | 275 |
| Positive control group | 95 |
| Test 1 group | 81 |
| Test 2 groups | 65 |
| Control test group | 85 |
As can be seen from the data in table 2 above, the phellinus igniarius sporophore extract of the present invention has a good uric acid lowering effect, and particularly, in the cultivation process of phellinus igniarius sporophore, the effective components are added to perform induced deposition, so that the obtained phellinus igniarius sporophore extract has a better uric acid lowering effect, and it is presumed that the effective components for lowering uric acid in the phellinus igniarius sporophore are efficiently accumulated, so that the phellinus igniarius sporophore has a better medicinal value.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (10)
1. A preparation method of an artificially cultured phellinus igniarius sporocarp extract for treating gout is characterized by comprising the steps of artificially culturing the phellinus igniarius sporocarp and extracting the phellinus igniarius sporocarp, and specifically comprises the following steps:
(1) preparing a cultivation bag material: taking 20-30 parts by weight of mulberry twig sawdust, 30-50 parts by weight of brewing waste residue, 10-30 parts by weight of molasses, 3-8 parts by weight of activated sludge particles, 1-5 parts by weight of quick lime and KH2PO40.2-0.5 weight part, and uniformly mixing to obtain the required culture medium; adding water into the culture medium, mixing uniformly, and bagging for later use;
(2) mycelium culture: inoculating a phellinus igniarius mother seed into the cultivation bag material, and carrying out constant-temperature cultivation on the inoculated cultivation bag material until the cultivation bag material is full of mycelia;
(3) and (3) fruiting body culture: cutting the bag material full of mycelia to produce mushrooms, continuously carrying out constant-temperature culture until the sporocarp is mature, and harvesting;
(4) pulverizing the phellinus igniarius sporocarp to obtain powder, adding an ethanol water solution for soaking and reflux extraction, collecting an extracting solution, concentrating under reduced pressure until no ethanol smell exists, and drying in vacuum to obtain the phellinus igniarius sporocarp extract required for artificial cultivation.
2. The method for preparing the artificially cultivated phellinus linteus fruiting body extract for treating gout according to claim 1, wherein in the step (1), the activated sludge granules are aerobic activated sludge granules.
3. The method for preparing the artificially cultivated phellinus linteus fruit body extract for treating gout according to claim 1 or 2, wherein in the step (1), the mass ratio of the cultivation substrate to water is 40-50%: 50 to 60 percent.
4. The method for preparing the cultured Phellinus linteus fruiting body extract for the treatment of gout as claimed in any one of claims 1-3, wherein in step (1), the culture medium further comprises a step of adding edible fungus extract as functional activity inducer.
5. The method for preparing the cultured Phellinus linteus fruiting body extract for treating gout as claimed in claim 4, wherein the edible fungus extract comprises Agrocybe aegerita extract.
6. The method for preparing fruiting body extract of artificially cultivated Phellinus linteus for treating gout as claimed in any one of claims 1 to 5, wherein in the step (2), the inoculation amount of Phellinus linteus mother species is 5-10 wt% of the cultivation medium.
7. The method for preparing the fruiting body extract of Phellinus linteus cultured artificially according to any one of claims 1 to 6, wherein in the step (2), the process conditions of the mycelium culturing step include: the culture temperature is controlled to be 25-30 ℃, the air humidity is 50-80%, and the illumination intensity is 10-20 lux.
8. The method for preparing the fruiting body extract of Phellinus linteus cultured artificially according to any one of claims 1 to 7, wherein in the step (3), the process conditions of the fruiting body culturing step include: the culture temperature is controlled to be 25-30 ℃, the air humidity is 70-80%, and the illumination intensity is 30-100 lux.
9. The method for preparing the fruiting body extract of Phellinus linteus cultured artificially according to any one of claims 1 to 8, for treating gout, wherein in the step (4):
the volume percentage content of the ethanol water solution is 60-80 v/v%;
the dosage ratio of the phellinus igniarius sporophore powder to the ethanol water solution is 10 g: 200 and 300 mL.
10. An artificially cultivated phellinus linteus fruit body extract for treating gout extracted by the method of any one of claims 1 to 9.
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| CN113079942A (en) * | 2021-04-08 | 2021-07-09 | 徐州工程学院 | Cultivation method and extraction method for increasing polyphenol content in phellinus igniarius sporocarp |
| CN113079941A (en) * | 2021-04-08 | 2021-07-09 | 徐州工程学院 | Cultivation method and extraction method for increasing flavonoid content in phellinus igniarius sporocarp |
| CN113079943A (en) * | 2021-04-08 | 2021-07-09 | 徐州工程学院 | Cultivation method and extraction method for increasing content of terpenoid in phellinus igniarius sporocarp |
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| CN113079943A (en) * | 2021-04-08 | 2021-07-09 | 徐州工程学院 | Cultivation method and extraction method for increasing content of terpenoid in phellinus igniarius sporocarp |
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