CN105875202A - Inoculation mechanism, strain containers and method for culturing edible mushrooms - Google Patents

Inoculation mechanism, strain containers and method for culturing edible mushrooms Download PDF

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Publication number
CN105875202A
CN105875202A CN201610419962.XA CN201610419962A CN105875202A CN 105875202 A CN105875202 A CN 105875202A CN 201610419962 A CN201610419962 A CN 201610419962A CN 105875202 A CN105875202 A CN 105875202A
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China
Prior art keywords
inoculation
cultivation
edible fungi
filter body
strain
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CN201610419962.XA
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Chinese (zh)
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蔡志闯
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蔡志闯
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Priority to CN201610419962.XA priority Critical patent/CN105875202A/en
Publication of CN105875202A publication Critical patent/CN105875202A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

Abstract

The invention provides an inoculation mechanism, strain containers and a method for culturing edible mushrooms, and belongs to the field of culturing of the edible mushrooms. The inoculation mechanism comprises an inoculation part and a culturing part, wherein an inoculation upper end opening is formed in the inoculation part, a culturing lower end opening is formed in the culturing part, the inoculation part and the culturing part are connected, and a filter body is arranged between the inoculation part and the culturing part. Harmful particles cannot pass through the filter body to enter culture mediums, but hyphae of strains can pass through the filter body to extend to the culture mediums to be cultured. Through the adoption of the inoculation mechanism, the production efficiency is improved, and the technical threshold is reduced. Each strain container provided by the invention comprises a bottle body and the inoculation mechanism, wherein each bottle body is connected with the culturing part. Through the adoption of the strain containers, rapid inoculation can be realized under bacterium environment, and the success rate is high. In addition, the method provided by the invention comprises the steps of preparing the strain containers and performing inoculation. The culturing method is simple, the rapid inoculation under bacterium environment can be realized, the production efficiency is improved, and the technical threshold is reduced.

Description

A kind of for cultivating the vaccination mechanism of edible fungi, strain container and method
Technical field
The present invention relates to the Cultivating techniques field in edible fungi, be used in particular to one Cultivate the vaccination mechanism of edible fungi, strain container and method.
Background technology
The cultivation of existing edible fungi needs just to carry out aseptic training under conditions of rigorous aseptic Supporting, sterile cultivating apparatus puts into big, technology requirement height, complex operation, inefficiency, produces Cost is high high with technical threshold.
Summary of the invention
It is an object of the invention to provide a kind of vaccination mechanism for cultivating edible fungi, overcome The aseptic culture that just can only complete under stringent asepsis requirements, substantially increases production efficiency, Reduce production cost and technical threshold.
Another object of the present invention is to provide a kind of strain container for cultivating edible fungi, profit Cultivate edible fungi with this strain container, production efficiency can be substantially increased, reduce production cost With technical threshold.
Another object of the present invention is to provide a kind of method for cultivating edible fungi, utilizing should Method can quickly be inoculated and cultivate, and improves production efficiency.
Embodiments of the invention are achieved in that
A kind of vaccination mechanism for cultivating edible fungi, is suitable to be connected with the container of dress culture medium, Including inoculation portion, cultivation portion and the filter body without nutrient, inoculation portion is provided with inoculation upper end open, Cultivation portion is provided with cultivation lower ending opening, inoculation portion and cultivation portion and connects, and cultivation portion is away from inoculation The one end in portion is connected with container, and filter body is arranged between inoculation portion and cultivation portion.
A kind of strain container for cultivating edible fungi, it includes bottle and for cultivating edible fungi Vaccination mechanism, be provided with the culture medium of edible bacteria growing in bottle, bottle is with cultivation portion even Connect.
A kind of method for cultivating edible fungi, comprising:
Prepare strain container: in bottle, load culture medium, then vaccination mechanism is set in institute State on bottle, make strain container, and to strain sterilizing containers;Wherein, vaccination mechanism includes Inoculation portion and cultivation portion, inoculation portion is provided with inoculation upper end open, and cultivation portion is provided with under cultivation End opening, is provided with the filter body without nutrient between inoculation portion and cultivation portion;And
Inoculation step: by edible fungi inoculation to inoculation portion, and cultivate.
The embodiment of the present invention provide for cultivate the vaccination mechanism of edible fungi, strain container with And method provides the benefit that: by the inoculation upper end open in inoculation portion by edible bacterial vaccine inoculation In inoculation portion, owing to filtering the filtration of body, particulates emission cannot pass through, and filter body is also simultaneously Be not provided that pathogenic bacteria rely growth material base, so particulates emission both cannot directly by filter Body, also cannot penetrate filter body to culture medium to sprout mode as mycelia, and mycelia can utilize The nutrition of himself carries out merisis and extends to culture medium, and mycelia can be led to filtering bodies and be entered The culture medium that cultivation portion connects, and absorb the nutrition of culture medium, carry out follow-up cultivation.This connects Planting the ingehious design of mechanism, enormously simplify the seeded process of edible fungi, overcoming can only be sternly The aseptic culture that just can complete under lattice aseptic condition, it is achieved have bacterium to inoculate, and success rate is high, greatly Improve greatly production efficiency, reduce production cost and technical threshold.Meanwhile, the present invention implements The strain container that example provides, it is connected with vaccination mechanism by bottle, it is simple to mycelia absorbs bottle Interior culture medium, utilizes this strain container real quickly to inoculate now with under collarium border, and success Rate is high.Additionally, the method being used for cultivating edible fungi that the embodiment of the present invention provides, strain is held After device completes, inoculating edible fungi in inoculation portion, this breeding method is simple, the behaviour of inoculation Make easily, the quick inoculation in collarium border can be realized, substantially increase production efficiency, reduce Production cost and technical threshold.
Accompanying drawing explanation
In order to be illustrated more clearly that the technical scheme of the embodiment of the present invention, below will be in embodiment The required accompanying drawing used is briefly described, it will be appreciated that the following drawings illustrate only this Some bright embodiment, is therefore not construed as the restriction to scope, common for this area From the point of view of technical staff, on the premise of not paying creative work, it is also possible to according to these accompanying drawings Obtain other relevant accompanying drawings.
Fig. 1 provides the knot being used for cultivating the vaccination mechanism of edible fungi for first embodiment of the invention Structure schematic diagram;
Fig. 2 provides the knot being used for cultivating the strain container of edible fungi for second embodiment of the invention Structure schematic diagram.
In figure:
Vaccination mechanism 100;
Inoculation portion 110;Inoculation upper end open 111;Inoculation lower ending opening 112;Inoculation dish 113; Through hole 114;Moisture film 115;
Cultivation portion 120;Cultivate upper end open 121;Cultivate lower ending opening 122;
Filter body 130;
Strain container 200;
Bottle 210.
Detailed description of the invention
For making the purpose of the embodiment of the present invention, technical scheme and advantage clearer, below will knot Close the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, Be fully described by, it is clear that described embodiment be a part of embodiment of the present invention rather than Whole embodiments.Generally herein described in accompanying drawing and the assembly of the embodiment of the present invention that illustrates Can arrange with various different configurations and design.
Therefore, detailed description not purport to the embodiments of the invention provided in the accompanying drawings below Limit the scope of claimed invention, but be merely representative of the selected enforcement of the present invention Example.Based on the embodiment in the present invention, those of ordinary skill in the art are not making creativeness The every other embodiment obtained under work premise, broadly falls into the scope of protection of the invention.
It should also be noted that similar label and letter expression similar terms in following accompanying drawing, therefore, The most a certain Xiang Yi accompanying drawing is defined, then need not it is carried out in accompanying drawing subsequently Definition and explanation further.
In describing the invention, it should be noted that term " on ", D score, " left ", Orientation or the position relationship of the instruction such as " right ", " length ", " width " are based on shown in the drawings Orientation or position relationship, or when this invention product uses, the orientation usually put or position are closed System, or the orientation that usually understands of those skilled in the art or position relationship, merely to just Describe in the description present invention and simplification rather than indicate or imply that the device of indication or element are necessary There is specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to this Bright restriction.Describe additionally, term " first ", " second " etc. are only used for distinguishing, and can not It is interpreted as instruction or hint relative importance.
In describing the invention, in addition it is also necessary to explanation, unless otherwise clear and definite regulation and limit Fixed, term " is arranged " and " connection " should be interpreted broadly, and connects for example, it may be fixing, Can also be to removably connect, or be integrally connected;Can be to be mechanically connected, it is also possible to be electricity Connect;Can be to be joined directly together, it is also possible to be indirectly connected to by intermediary, can be two The connection of element internal.For the ordinary skill in the art, can manage with concrete condition Solve above-mentioned term concrete meaning in the present invention.
First embodiment
Refer to Fig. 1, present embodiments provide a kind of vaccination mechanism for cultivating edible fungi 100, this vaccination mechanism 100 can be connected with container, it is simple to inoculates edible fungi and trains Supporting, container content has the culture medium of edible bacteria growing, and this vaccination mechanism 100 includes inoculation portion 110, cultivation portion 120 and filter body 130, inoculation portion 110 and cultivation portion 120 connect, and cultivate Portion 120 is connected with container away from the one end in inoculation portion 110, and filter body 130 is arranged on inoculation portion Between 110 and cultivation portion 120.
Inoculation portion 110 is provided with inoculation upper end open 111 and inoculation lower ending opening 112, and connects Planting upper end open 111 is horn opening, and its internal diameter is more than the internal diameter of inoculation lower ending opening 112. The internal diameter of inoculation upper end open 111 is without being strict with, and it is highly maintained at more than 20mm.
Being provided with inoculation dish 113 in the present embodiment in inoculation portion 110, inoculation dish 113 is arranged on The top of filter body 130, when being inoculated to inoculation portion 110 by inoculation upper end open 111, will Strain is inoculated on inoculation dish 113, in the present embodiment, inoculation dish 113 is provided with multiple logical Hole 114, the mycelia of edible fungi is worn filtering bodies 130 by this through hole 114, thus absorbs cultivation Nutrition in base, and fungal spore and antibacterial can not lead to filtering bodies 130, logical filtering bodies 130 will Fungal spore and antibacterial completely cut off outside culture medium, it is achieved that have the aseptic culture under bacterium state, The method greatly simplifiing inoculation, improves production efficiency simultaneously, reduce production cost with Technical threshold.
Specifically, in the present embodiment a diameter of 2~4mm of the through hole 114 on inoculation dish 113, When the diameter of through hole 114 is more than 4mm, leakiness strain, and make the strain pressure of inoculation dish 113 Making a concerted effort to conduct makes it deform to filter body 130, comes off, loses filtration.Through hole 114 distance The distance at the edge of inoculation dish 113 need to be more than 3mm, and this design can be at inoculation dish 113 or connect When kind portion 110 deforms under the conditions of external force, play certain cushioning effect, it is to avoid strain With inoculation portion 110 or the sidewall contact of inoculation dish 113, can prevent leakage from carrying disease germs air simultaneously. Certainly, in other embodiments, the number of the through hole 114 on inoculation dish 113 can regard inoculation Depending on the size of mechanism 100 or inoculation dish 113.
The present embodiment is filtered body 130 and is positioned at the lower section of inoculation dish 113, and the thickness of filter body 130 It is 4~5mm, by gravity, Electrostatic Absorption after harmful bacteria microgranule enters fibrous airstream stagnant area Effect stopped by fiber, and edible fungi can rely on own growth penetrate filter body to culture medium, The thickness of 4~5mm can effectively filter out particulates emission, and strain is easy to logical filtering bodies 130 simultaneously Stretch into culture medium;Filter the internal diameter 1mm bigger than the internal diameter of inoculation dish 113 of body 130 simultaneously, by Relatively big in filter body 130 elasticity, beneficially clamping inoculation dish 113, and by the edge of inoculation dish 113 Seal.The distance of the bottom surface distance inoculation lower ending opening 112 of filter body 130 is 5~8mm.
In the present embodiment, inoculation portion 110 is provided with moisture film 115, and this moisture film 115 is mainly used In covering tightly inoculation upper end open 111, rather than positive confinement, to ensure the humidity of kind of block.
Cultivation portion 120 is provided with cultivation upper end open 121 and cultivates lower ending opening 122, wherein, Cultivate upper end open 121 to be connected with inoculation lower ending opening 112, cultivate lower ending opening 122 and training Support base to connect.
The height in cultivation portion 120 is 15~20mm, and wherein cultivation portion 120 is near cultivating upper end The part of opening 121 is connected with inoculation lower ending opening 112, and the height connected is 5~8mm, I.e. when inoculating lower ending opening 112 and being set in the outside cultivating upper end open 121, cultivate upper end Opening 121 is along cultivating upper end open 121 to the direction cultivating lower ending opening 122 The part of 5~8cm, with the distance of the bottom surface distance inoculation lower ending opening 112 of filter body 130 be 5~8mm is the most corresponding, and the edge i.e. cultivating upper end open 121 props up the bottom surface of filter body 130.
Cultivation portion 120 is near cultivating the part of lower ending opening 122 for being connected with culture medium, just Lead to filtering bodies 130 in strain and enter lower space, eventually enter into culture medium, absorb in culture medium Nutrition.Owing to the energy for growth of mycelia can weaken, i.e. mycelia along with the increase of growth distance Length the shortest more good, the longest can injure mycelia, cause mycelia vitality to decline.The present embodiment In, it is preferable that after inoculation portion 110 is connected with cultivation portion 120, filter body 130 distance from bottom training The distance supporting lower ending opening 122 is 15mm.
The distance of growth includes that the thickness filtering body 130 and filter body 130 are to cultivating lower ending opening The required growth distance of the distance of 122, i.e. strain is 19~20mm, now can guarantee that mycelia Preferably energy for growth.
In the present embodiment, the internal diameter in cultivation portion 120 is along cultivating upper end open 121 to cultivating lower end The direction of opening 122 is gradually increased, and the internal diameter making cultivation lower ending opening 122 is big, and can hold The power being subject to also becomes big, it is simple to keep the balance in cultivation portion 120;Cultivate lower ending opening 122 simultaneously Internal diameter big, in " eight " font opening, can effectively prevent cultivating lower ending opening 122 and be absorbed in training Support in base, simultaneously work as supporting role, it is ensured that culture medium separates with filter body 130.
Filter body 130 is to use geotextiles to make, it is preferred to use 600~800g/m2Geotextiles, Preferred plan is for using 800g/m2Geotextiles make in the embodiment of the present invention provide filter body 130.Owing to geotextiles has good water-permeable and air permeable, without nutrient substance on this filter body 130, Its microbial resistance is good, and owing to filtering the filtration of body 130, particulates emission cannot pass through, with Time filter body 130 can not provide pathogenic bacteria rely growth material base, so particulates emission both without Method directly leads to filtering bodies 130, also cannot penetrate filter body 130 to training to sprout the mode as mycelia Support base.But owing to the mycelia of edible fungi can be grown by the nutritional labeling of himself, and And extend to the direction in cultivation portion 120, the length that mycelia generally can grow is longer, Under growth conditions appropriate state, most of kind kind truffles silks can climb wall grow 2 centimetres with On, but the growth of mycelia is the longest, and its mycelia vitality is the lowest, is more unfavorable for the training of edible fungi Supporting, the distance between the culture medium that the filter body 130 in the present embodiment to cultivation portion 120 connects is 19~20mm, it is much smaller than the growth length of mycelia, 19~20mm set in the present embodiment Distance does not affect mycelia normal growth to culture medium, and absorbs the nutrition in culture medium, raw for it Long.
Referring to Fig. 1, inoculation portion 110 and cultivation portion 120 can be an entirety, it is also possible to For Split type structure, in the present embodiment, preferably inoculation portion 110 and cultivation portion 120 are Split type structure, And inoculate lower ending opening 112 and can be set in the outside cultivating upper end open 121, form one The form of individual sheathed clamping, by being set in cultivation portion 120 in inoculation portion 110, makes filter body 130 form the space of a relative closure to cultivation portion 120, owing to filter body 130 has hole, It is possible to permeable and ventilative, meet the oxygen needed for the growth of edible fungi.
Concrete, in the present embodiment, the internal diameter of the inoculation upper end open 111 in inoculation portion 110 is 40mm, the internal diameter of inoculation lower ending opening 112 is 32mm, and the internal diameter filtering body 130 is also 32mm, the internal diameter of the cultivation upper end open 121 in cultivation portion 120 is 31.6mm, under cultivation The internal diameter of end opening 122 is 40mm, and it is big that inoculation portion 110 forms inoculation upper end open 111, The horn cylindrical that inoculation lower ending opening 112 is little, and cultivation portion 120 is formed and cultivates upper end open 121 is little, cultivates the cylinder of the big inverted tubaeform of lower ending opening 122.The cultivation in cultivation portion 120 The stability in lower ending opening 122 ambassador's cultivation portion 120 is more preferable.Certainly, in other embodiments, Inoculation portion 110, filter body 130 and cultivation portion 120 internal diameter size can according to customer demand, Produce many considering such as kind and cost to select, and be not limited in the present embodiment The size range provided.Vaccination mechanism 100 can be the upper shield of container, it is also possible to for container Lid, it is also possible to be other various structures.In the present embodiment, preferred vaccination mechanism 100 is container Upper shield, by being set on container, thus realize and the connection of container.
Refer to Fig. 1, the vaccination mechanism 100 for cultivating edible fungi that the present embodiment provides Principle is:
Carrying out the inoculation under open state in inoculation portion 110, the strain of edible fungi falls in inoculation On dish 113, and enter filter body 130 by the through hole 114 on inoculation dish 113, due to filter Fungal spore, antibacterial can be played the effect of filtration sterilization by the structure of body 130 densification, and Filter body 130 nutrition needed for fungal spore, bacterial growth cannot be provided, so fungal spore and Antibacterial cannot filter growth and breeding on body 130, it is impossible to sprouts and wears filtering bodies 130 for mycelia, and For the mycelia of edible fungi, it can utilize the nutrient substance of himself to grow, and And towards the direction extension of culture medium.
Wherein, the mechanism of filter body 130 filtration sterilization is:
Inertial collision: owing to filter body 130 is made up of staggered fiber, staggered fiber constantly changes Change direction, during bypass gas flow, rubbed adhesion owing to microgranule inertia hits fiber;
Intercept and be detained: when air velocity is down to stagnant area under critical, and microgranule is slowly near quilt Fiber traps;
Gravitational settling: gravity is more than sedimentation absorption during other external force such as air-flow;
Electrostatic Absorption: dry air produces electric charge and the synthetic fibers charges of different polarity with microgranule friction, inhales Drawing coupling, wherein, microorganism particle is many with electric charge.
After inoculating strain in inoculation portion 110, owing to inoculating under open environment, so inoculation Portion 110 is contained within part particulates emission, such as: fungal spore and antibacterial.When particulates emission is big When 0.3um, mainly do inertia motion, and when particulates emission is less than 0.1um, mainly do Brownian movement, from point of theory, particulates emission (fungal spore and antibacterial) is 0.5~10um, its It is substantially carried out inertia motion, so Brownian movement is not discussed by the embodiment of the present invention.
Particulates emission when together moving with air-flow, air-flow continuous side of change in staggered fiber To, particulates emission hits fiber due to inertial collision, and particulates emission can be rubbed suction by filter body 130 Attached;Entering behind fiber retention district when air-flow, microgranule is slowly captured near fiber simultaneously, also by In particulates emission by electrostatic and action of gravity, and by fibers adsorption.And hypha of edible fungus relies on and divides Schizogenesis is long, utilizes the nutrient of himself the most forwards to extend, hypha of edible fungus energy Gravity and electrostatic force is enough overcome to lead to filtering bodies 130 until culture medium.The present embodiment is by carrying Edible fungi is carried out Open-Architecture Controller, in seeded process for vaccination mechanism 100, it is not necessary to Inoculate in the environment of rigorous aseptic, simultaneously in follow-up incubation, which avoids The operations such as Xie Kou, tying or upper cover, the vaccination mechanism 100 using the present embodiment to provide carries out people Work inoculation is artificial eye-liquid inoculation speed 2 times in prior art, is artificial solid in prior art 40 times of inoculation speed, and have higher success rate.
Compared in prior art, the aseptic culture that just can need to complete at strict aseptic condition, Its equipment investment is big, technology requires height, complex operation, inefficiency;And the present embodiment realizes There is the aseptic culture under bacterium state, substantially increase production efficiency, reduce production cost and skill Art threshold.And output and quality penetration and promotion high, easy, and can whole year production.
In the present embodiment, inoculation portion 110 and cultivation portion 120 are tubular, and filter body 130 is circle The tabular of shape, and in other embodiments, inoculation portion 110, cultivation portion 120 and filter body 130 Can be square or other are variously-shaped, and its size is adjusted also dependent on concrete condition.
Repeatedly proving through the fact, far above sterilizing room inoculation, (sterilizing room works success rate of the present invention Under state generating capacity be 500~1000/people/minute), its average pollution rate is 5~10%, and The present invention there is not yet pollution.
It addition, the present invention and other modes of production have the detoxification function that can not possess, mycelia is by filtering Body 130 underlying space grows to culture medium.
In a word, this technique can be greatly improved inoculation efficiency, and it is production technology liquid inoculation speed 2 times, industrialization solid vaccination 40 times, the present invention need not open bottleneck or bag mouth, is Upper bottleneck or bag mouth, activity, not by any restriction, need not overlap glove, aseptic take work.
Investment in fixed assets is almost without comparability, and except having filter body and moisture film part, conversion is to often Bag meter, for equal solid Hand Inoculations, only need to inoculate speed lifting 10% and can receive Return cost.
Cost of the present invention is extremely low, ultrahigh in efficiency, and yield and quality is excellent, and may replace fruiting ring, holds concurrently Having detoxification, especially without technical threshold, pole is beneficial to penetration and promotion, during it has thoroughly been dissolved, The difficult problems such as high-grade mushroom grog production bottleneck (inoculate careful, loaded down with trivial details, easy infection, efficiency are low), It can directly be transported to send out the inoculation of bacterium room simultaneously, eliminates the operation carried back and forth, and meaning is huge.
Second embodiment
Refer to Fig. 1 and Fig. 2, the present embodiment provides a kind of bacterium for cultivating edible fungi Kind of container 200, it include that bottle 210 and first embodiment of the invention provide for cultivating food With the vaccination mechanism 100 of bacterium, in bottle 210, it is provided with the culture medium of edible bacteria growing, bottle Body 210 is connected with cultivation portion 120.
By vaccination mechanism 100 to be connected formation strain container 200 with bottle 210, available Edible fungi is directly cultivated by strain container 200, more convenient to operate.Bottle 210 simultaneously Shapes and sizes can be with situation depending on, this bottle 210 can be various types of bottle 210, Can also be bag, by loading culture medium in bag, then by bag and vaccination mechanism 100 connect, and also can form strain container 200.
It is connected with vaccination mechanism 100 by bottle 210, it is simple to mycelia absorbs in bottle 210 The nutrient substance of culture medium, utilizes this strain container 200 real quickly to connect now with under collarium border Kind, and success rate is high.Strain container 200 can simplify the operation cultivating edible fungi, makes inoculation It is easier to, the quick inoculation in collarium border can be realized, substantially increase production efficiency, reduce Production cost and technical threshold.
3rd embodiment
Refer to Fig. 1 and Fig. 2, the present embodiment provides a kind of side for cultivating edible fungi Method, it comprises the following steps:
S1, prepare strain container 200.
Culture medium, the inoculation then first embodiment of the invention provided are provided in bottle 210 Mechanism 100 is set on bottle 210, makes the strain container that second embodiment of the invention provides 200, and to strain container 200 sterilizing.
Specifically, the present invention selects edible mushroom cultivating bag as bottle 210, plant to edible fungi Training bag adds culture medium, then by cultivation portion 120 and the edible fungus culturing of vaccination mechanism 100 Bag connects, and wherein cultivation portion 120 is hollow tubular, and it cultivates lower ending opening 122 relatively greatly, Edible mushroom cultivating bag stretches in cultivation portion 120 by cultivating lower ending opening 122, and through cultivating Upper end open 121, then outwardly and downwardly turns down edible mushroom cultivating bag, exposes cultivation portion 120 Inner passage.Then will be set in the inoculation portion 110 of filter body 130 and inoculation dish 113 The outside in cultivation portion 120, i.e. makes strain container 200, is then entered by this strain container 200 Row sterilizing.Wherein, the selection of culture medium and the concrete operations of sterilizing can refer to prior art.
The embodiment of the present invention before sterilization, at inoculation upper end open 111 upper cover in inoculation portion 110 Lastblock damp-proof membrane (not shown), is then fixed on damp-proof membrane in inoculation portion 110 with rubber band, Carrying out sterilizing the most again, this damp-proof membrane is prevented from filtering body 130 and is subject to when high moisture vapor sterilizing Tide, simultaneously, moreover it is possible to reach the purpose of moisturizing.
When putting sterilizing owing to crouching, often have minority bacterium bag moisture distribution inequality and flow to filter body, Cause infecting, for avoiding the generation of this situation, to placing filter body 130 in inoculation portion 110 Inner ring (not shown) is set up in position, one end of inner ring with filter body 130 be connected, the other end towards Inoculation lower ending opening 112, the effect of inner ring is the probability of isolation filter body 130 and contact with moisture. The height of inner ring is 8mm, and it is 4mm with the distance in the outside in inoculation portion 110.
Some kind needs to use heavy caliber fruiting to iris out mushroom, can use large-sized connecing accordingly Planting mechanism 100, the cultivation portion 120 that the present invention provides can be used as fruiting circle, for preventing further Sleeping bacterium bag of putting collapses material and contacts filter body 130, can on the set of inner ring bottom a netted Ka Gai (figure Do not show).
S2, inoculation step.
By on edible fungi inoculation to inoculation portion 110, it is not necessary to connect in the environment of rigorous aseptic Kind, simultaneously without routine operations such as Xie Kou, tying or upper covers, only damp-proof membrane need to be opened, then Putting into kind of a block, this inoculation step is simple, can quickly inoculate, greatly substantially increase life Produce efficiency, reduce production cost and technical threshold.
Use of the right age strain, it is not necessary to any form is sterilized.Natural environment.The most movable inoculation, it may be assumed that Directly capture kind of a block with hands and put in inoculation portion 110, preferably strain is filled up inoculation portion 110.
S3, membrane step.
After inoculation step completes, the upper surface at strain container 200 draws last layer moisture film 115.This moisture film 115 can keep edible fungi desired moisture level in strain container 200, favorably In the growth of quickening edible fungi, if environment is the driest, can water to bag bottom wet.As Fruit needs to accelerate to send out bacterium, can loosen inoculation portion 110 and cultivation portion after mycelia covers with surface The seam of 120.
Specifically, this moisture film 115 can be independently arranged on strain container 200, i.e. arranges In inoculation portion 110 near the position of inoculation upper end open 111, this moisture film 115 can also be During production, after inoculation terminates in batch, drawing a bigger moisture film 115, it can cover multiple Strain container 200.
In the present embodiment, preferably draw moisture film 115 by unified for multiple strain containers 200, save time Laborsaving, simultaneously the most cost-effective, can use the most common mulch film etc. as moisture film 115, Its low cost, same membrane is simple to operate.
Certainly, in other embodiments, this membrane step can also be omitted.
S4, incubation step.
After membrane step completes, can carry out the cultivation of routine, fruiting, this step refers to existing There is technology.
Cultivate edible fungi by utilizing strain container 200 to carry out, simplify the cultivation side of edible fungi Method, the cultivation making edible fungi is simpler, and operation is more prone to.This vaccination mechanism 100 simultaneously Reusable repeatedly only need to change filter body every time, with low cost.
In sum, the present embodiment will be edible by the inoculation upper end open 111 in inoculation portion 110 Bacterium strain is inoculated in inoculation portion 110, and strain eventually falls on filter body 130, due to filter body The filtration of 130, particulates emission cannot pass through, and filter body 130 can not provide pathogenic bacteria simultaneously Rely the material base of growth, so particulates emission both cannot directly lead to filtering bodies, also cannot be with The mode for mycelia of sprouting penetrates filter body 130 to culture medium, so when inoculation, it is not necessary in nothing Inoculating under collarium border, present in environment, fungal spore and antibacterial cannot pass through on filter body 130 Filter body 130, and mycelia can utilize the nutrition of himself carry out merisis and prolong to culture medium Stretching, mycelia can be led to filtering bodies 130 and be entered the culture medium that cultivation portion 120 connects, and absorbs training Support the nutrition of base, carry out follow-up cultivation.The ingehious design of this vaccination mechanism 100, significantly letter Change the seeded process of edible fungi, overcome the nothing that just can only complete under stringent asepsis requirements Bacterium is cultivated, it is achieved have bacterium to inoculate, and success rate is high, substantially increases production efficiency, reduces Production cost and technical threshold.Meanwhile, strain container 200 is by loading training in bottle 210 Support base, be then connected with vaccination mechanism 100, it is simple to mycelia absorbs the culture medium in bottle 210 Nutrition, utilize this strain container 200 real quickly to inoculate now with under collarium border, and success Rate is high.Additionally, utilize strain container 200 to carry out cultivating edible fungi, simplify cultivation edible fungi Method, make inoculation be easier to, can realize quickly inoculating of collarium border, substantially increase life Produce efficiency, reduce production cost and technical threshold.
Compared with prior art, present invention have the advantage that
(1) aseptic culture under the present embodiment achieves bacterium state.
(2) greatly improve production efficiency, reduce inoculation technique threshold, not by aseptic behaviour Make and environmental constraints.
(3) filter body 130 has detoxification, cultivation portion 120 can double as fruiting ring.
(4) can directly be transported to culturing room after completing sterilizing complete quickly to inoculate, it is not necessary to inoculation Room transit link back and forth.
(5) without sterilizing room and various sterilization with staticize equipment, cost puts into extremely low.
(6) reason mycelia penetrate filtering layer do not deposit to culture medium operation and inoculation gnotobasis carry disease germs Cause polluting, have higher success rate.
(7) sterile culture, yield and quality is the most excellent.
The vaccination mechanism that the present invention provides can be applicable to Pleurotus ostreatus, Pleurotus eryngii, Flammulina velutiper (Fr.) Sing etc..
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, For a person skilled in the art, the present invention can have various modifications and variations.All at this Within the spirit of invention and principle, any modification, equivalent substitution and improvement etc. made, all should Within being included in protection scope of the present invention.

Claims (10)

1., for cultivating a vaccination mechanism for edible fungi, be suitable to and the container of dress culture medium Connect, it is characterised in that include inoculation portion, cultivation portion and the filter body without nutrient, described in connect Kind portion is provided with inoculation upper end open, and described cultivation portion is provided with cultivation lower ending opening, described in connect Kind portion and described cultivation portion connect, and described cultivation portion is away from the one end in described inoculation portion and described appearance Device connects, and described filter body is arranged between described inoculation portion and described cultivation portion.
Vaccination mechanism for cultivating edible fungi the most according to claim 1, it is special Levying and be, be provided with inoculation dish in described inoculation portion, described inoculation dish is arranged on described filter body Top, described inoculation dish is provided with multiple through hole.
Vaccination mechanism for cultivating edible fungi the most according to claim 1, it is special Levying and be, described inoculation portion is provided with the moisture film for covering tightly described inoculation upper end open.
Vaccination mechanism for cultivating edible fungi the most according to claim 1, it is special Levying and be, described inoculation portion and described cultivation portion are Split type structure, and described inoculation portion is additionally provided with Inoculation lower ending opening, described cultivation portion is additionally provided with cultivation upper end open, and described inoculation lower end is opened Mouth is optionally set in the outside of described cultivation upper end open, described filter body be arranged on described in connect Kind portion is near the position of described inoculation lower ending opening.
Vaccination mechanism for cultivating edible fungi the most according to claim 4, it is special Levying and be, described inoculation upper end open is horn opening, the internal diameter of described inoculation upper end open Internal diameter more than described inoculation lower ending opening.
Vaccination mechanism for cultivating edible fungi the most according to claim 4, it is special Levying and be, the internal diameter in described cultivation portion is along described cultivation upper end open to described cultivation lower ending opening Direction be gradually increased.
Vaccination mechanism for cultivating edible fungi the most according to claim 1, it is special Levying and be, described filter body is made up of geotextiles.
8. the strain container being used for cultivating edible fungi, it is characterised in that it includes bottle Body and the vaccination mechanism for cultivating edible fungi as described in any one of claim 1~7, described Being provided with the culture medium of edible bacteria growing in bottle, described bottle is connected with described cultivation portion.
9. the method being used for cultivating edible fungi, it is characterised in that comprising:
Prepare strain container: in bottle, load culture medium, then vaccination mechanism is set in institute State on bottle, make strain container, and to described strain sterilizing containers;Wherein, described inoculation Mechanism includes that inoculation portion and cultivation portion, described inoculation portion are provided with inoculation upper end open, described training Foster portion is provided with cultivation lower ending opening, is provided with without battalion between described inoculation portion and described cultivation portion Support the filter body of thing;And
Inoculation step: by edible fungi inoculation to described inoculation portion, and cultivate.
Method for cultivating edible fungi the most according to claim 9, its feature exists In, described inoculation step is included on edible fungi inoculation to described inoculation portion, then draws Film step, is then incubated for, described membrane step: the upper surface at described strain container pulls on one Layer moisture film.
CN201610419962.XA 2016-06-13 2016-06-13 Inoculation mechanism, strain containers and method for culturing edible mushrooms Pending CN105875202A (en)

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