CN206866150U - Aseptic seedlings of plants culture apparatus is used in a kind of endophyte tieback experiment - Google Patents
Aseptic seedlings of plants culture apparatus is used in a kind of endophyte tieback experiment Download PDFInfo
- Publication number
- CN206866150U CN206866150U CN201720672348.4U CN201720672348U CN206866150U CN 206866150 U CN206866150 U CN 206866150U CN 201720672348 U CN201720672348 U CN 201720672348U CN 206866150 U CN206866150 U CN 206866150U
- Authority
- CN
- China
- Prior art keywords
- bottle
- opening
- endophyte
- tieback
- culture apparatus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000002474 experimental method Methods 0.000 title claims abstract description 22
- 239000012528 membrane Substances 0.000 claims abstract description 11
- 241000894006 Bacteria Species 0.000 claims abstract description 10
- 239000012780 transparent material Substances 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 abstract description 35
- 238000001914 filtration Methods 0.000 abstract description 4
- 235000015097 nutrients Nutrition 0.000 abstract description 4
- 230000012010 growth Effects 0.000 abstract description 3
- 239000013589 supplement Substances 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000008635 plant growth Effects 0.000 description 5
- 238000005070 sampling Methods 0.000 description 5
- 239000011159 matrix material Substances 0.000 description 4
- 230000001766 physiological effect Effects 0.000 description 3
- 230000000721 bacterilogical effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 240000003553 Leptospermum scoparium Species 0.000 description 1
- 235000015459 Lycium barbarum Nutrition 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
本实用新型公开了一种内生菌回接实验用植物无菌苗培养装置，其包括瓶体和瓶盖，所述瓶盖设置于瓶体上，所述瓶盖上设置有第一开口，靠近瓶盖外缘一侧的第一开口端部设置有虑菌透气膜，所述瓶体上设置有第二开口，靠近瓶体外缘一侧的所述第二开口的端部设置有虑菌透气膜。本实用新型的目的是旨在提供一种利于直根系植物生长，便于补充营养物质，实验时操作方便的内生菌回接实验用植物无菌苗培养装置。
The utility model discloses a device for cultivating aseptic plant seedlings for endophytic bacteria reconnection experiments, which comprises a bottle body and a bottle cap, the bottle cap is arranged on the bottle body, and a first opening is arranged on the bottle cap. The end of the first opening near the outer edge of the bottle cap is provided with a bacteria-filtering gas-permeable membrane, the bottle body is provided with a second opening, and the end of the second opening near the outer edge of the bottle is provided with a bacteria-filtering membrane. Breathable membrane. The purpose of the utility model is to provide a plant sterile seedling cultivation device for endophytic bacteria reconnection experiments which is beneficial to the growth of tap root plants, convenient to supplement nutrients, and convenient to operate during experiments.
Description
Technical field
The utility model belongs to sterile cultivating apparatus technical field, is specifically related to a kind of endophyte tieback experiment and uses plant
Aseptic seedling culture device.
Background technology
Plant culture bottle now used is mainly vial and PC plastic bottle, has square, and cylinder and taper etc. are several
Shape, variously-shaped is all to have lid in surface, and bacteriological filtration ventilated membrane is generally equipped with lid and is communicated with the external world, the rule of blake bottle
Lattice number is more.
Influenceed for what research endophyte of plant grew to plant host, it is necessary to isolated endophyte of plant pure culture
Thing tieback is in sterile plant, to analyze shadow of the endophyte to plant growth, physiological property, disease resistance and gene expression etc.
Ring.Existing plant culture bottle only has lid in surface, is operated not when tieback experiment is carried out by plant roots and stem
Just, bacterium solution easily pollutes its hetero-organization such as blade.Simultaneously because vegetation growth state differs greatly, returning for xylophyta is being carried out
When connecing experiment, plant cultivation cycle is longer, needs to cultivate more than 5-6 months sometimes, it is necessary to nutrient turnover material.And tissue-cultured seedling
Most cultivation cycles are relatively short, and used tissue culture flasks volume is relatively small, is unfavorable for the plant growth of some systems of taproot,
It is inconvenient for use for cycle longer experiment.
Utility model content
For above-mentioned weak point of the prior art, the purpose of this utility model is intended to offer one kind and is beneficial to the system of taproot
Plant growth, is easy to extra-nutrition material, during experiment easy to operate endophyte tieback experiment use aseptic seedlings of plants culture apparatus.
In order to solve the above-mentioned technical problem, the utility model is achieved through the following technical solutions：A kind of endophyte returns
Experiment aseptic seedlings of plants culture apparatus is connect, it includes bottle and bottle cap, and the bottle cap is arranged on bottle, is set on the bottle cap
The first opening is equipped with, the first open end close to bottle cap outer rim side, which is provided with, considers bacterium ventilated membrane, is provided with the bottle
Second opening, have close to the end set of second opening of bottle outer rim side and consider bacterium ventilated membrane.
Preferably, the quantity of second opening is at least one, and the internal diameter numerical value of the second opening is less than the first opening
Internal diameter numerical value.
Preferably, the quantity of second opening is 3, the centerlines between two neighboring second opening
For 120 °.
Preferably, the bottle is made up of transparent material.
Preferably, the cross section of bottle structure in a ring.
Cultivate aseptic plant and carry out endophyte tieback experiment, plant needs to be incubated in suitable device, should kept away
Exempt from external source living contaminants, also to ensure that plant can obtain enough illumination, moisture and nutriment, growth of keeping fit, and
Will also be suitably from different parts tieback endophytes such as the leaves, stem and root (soil) of plant；Will be periodically from plant after endophyte tieback
Different parts sampling is detected, and analyzes the colonisation of endophyte and the shadow to each side such as plant growth, physiological properties
Ring.
For the utility model, the cross section of bottle is annular, and the internal diameter of bottle is 10-15cm, with bottle body bottom
On the basis of line, 3 second openings are being equidistantly positioned by 120 degree of angles apart from the position of bottle body bottom 10cm height, to train
During supporting plant, staff supplements all kinds of nutriments into the culture matrix inside bottle, and passes through sterile working
Root, stem's position tieback endophyte and plant spot sampling to plant；Bottle body bottom to the height of bottle openend is 20-
25cm；The first opening is provided with bottle cap, it is used to load culture matrix, plants aseptic seedling and the life into plant leaf tieback
Bacterium, the regular sampling of leaf tissue is carried out, bacteriological filtration ventilated membrane is respectively mounted in the end of the first opening and the second opening, inside bottle
Communicated by the first opening and the second opening with the external world.
Bottle can be selected according to the floristics of culture, can meet the needs for cultivating different type aseptic seedlings of plants；
Staff can be facilitated to carry out sterile working in incubation simultaneously, to endophyte tieback, periodically sampling detection and culture
The change of matrix, the supplement of nutriment etc. all can be carried out conveniently, and cannot be easily caused pollution., can because bottle volume is larger
More nutrient matrix is disposably put into, can be supplemented the nutrients in incubation by the way that the second opening is appropriate, therefore be more adapted to
The culture plant seedlings of long period, it is easy to study the correlation of endophyte and plant host.
The endophyte tieback experiment effect that several draft and xylophyta have been carried out using the utility model is good.
Carried out endogeny rayungus tieback candidum tissue culturing seedling using the utility model, endogeny rayungus tieback Chinese cabbage without
Vaccine, green vegetables aseptic seedling, and the experiment such as endogeny rayungus tieback Dali tea tree aseptic seedling, cultivation cycle are 2-5 months.
In experimentation, plant pollution probability is smaller, and endophyte of plant is have detected to plant growth spy by periodically sampling
The influence of property, physiological property, resistance etc., experimental plants and adjoining tree significant difference, achieve preferable experiment effect.
Second opening is set on bottle, easily can sample or add fertilizer from plant different parts without easily causing pollution, and
Second opening can also increase throughput, therefore be one more suitable for the correlation of research endophyte of plant and plant host
Device.
Brief description of the drawings
Fig. 1 is the utility model structure diagram.
Embodiment
As shown in figure 1, aseptic seedlings of plants culture apparatus is used in a kind of endophyte tieback experiment, it includes bottle 1 and bottle cap 2,
The bottle cap 2 is arranged on bottle 1, and the first opening 3 is provided with the bottle cap 2, the first opening close to the outer rim side of bottle cap 2
3 end sets, which have, considers bacterium ventilated membrane 4, and the second opening 5 is provided with the bottle 1, and described second close to the outer rim side of bottle 1
The end set of opening 5, which has, considers bacterium ventilated membrane 4；The quantity of second opening 5 is at least one, the internal diameter number of the second opening 5
Value is less than the internal diameter numerical value of the first opening 3；The quantity of second opening 5 is 3, between two neighboring second opening 5
Centerlines be 120 °；The bottle 1 is made up of transparent material；The cross section of the bottle 1 structure in a ring.
Claims (5)
1. a kind of endophyte tieback experiment uses aseptic seedlings of plants culture apparatus, including bottle and bottle cap, the bottle cap to be arranged at bottle
On body, the first opening is provided with the bottle cap, the first open end close to bottle cap outer rim side, which is provided with, considers bacterium ventilated membrane,
Characterized in that, the second opening is provided with the bottle, close to the end set of second opening of bottle outer rim side
Have and consider bacterium ventilated membrane.
2. aseptic seedlings of plants culture apparatus is used in a kind of endophyte tieback experiment according to claim 1, it is characterised in that institute
The quantity for stating the second opening is at least one, and the internal diameter numerical value of the second opening is less than the internal diameter numerical value of the first opening.
3. aseptic seedlings of plants culture apparatus is used in a kind of endophyte tieback experiment according to claim 2, it is characterised in that institute
The quantity for stating the second opening is 3, and the centerlines between two neighboring second opening are 120 °.
4. aseptic seedlings of plants culture apparatus is used in a kind of endophyte tieback experiment according to claim 1, it is characterised in that institute
Bottle is stated to be made up of transparent material.
5. aseptic seedlings of plants culture apparatus is used in a kind of endophyte tieback experiment according to claim 1, it is characterised in that institute
State the cross section of bottle structure in a ring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720672348.4U CN206866150U (en) | 2017-06-10 | 2017-06-10 | Aseptic seedlings of plants culture apparatus is used in a kind of endophyte tieback experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720672348.4U CN206866150U (en) | 2017-06-10 | 2017-06-10 | Aseptic seedlings of plants culture apparatus is used in a kind of endophyte tieback experiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206866150U true CN206866150U (en) | 2018-01-12 |
Family
ID=61332918
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720672348.4U Expired - Fee Related CN206866150U (en) | 2017-06-10 | 2017-06-10 | Aseptic seedlings of plants culture apparatus is used in a kind of endophyte tieback experiment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206866150U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108541514A (en) * | 2018-03-21 | 2018-09-18 | 丽水市林业科学研究院 | A method of cultivating orange newborn mushroom offspring |
| CN110447471A (en) * | 2019-07-31 | 2019-11-15 | 江南大学 | Plant cultivation device is used in a kind of experiment of blade inoculation endophyte of plant |
| CN110476648A (en) * | 2019-08-02 | 2019-11-22 | 江南大学 | A kind of root-pouring method inoculated plant endophyte experiment sterile culture device |
-
2017
- 2017-06-10 CN CN201720672348.4U patent/CN206866150U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108541514A (en) * | 2018-03-21 | 2018-09-18 | 丽水市林业科学研究院 | A method of cultivating orange newborn mushroom offspring |
| CN108541514B (en) * | 2018-03-21 | 2023-10-13 | 丽水市农林科学研究院 | Method for cultivating orange Rumex Lactarius mycorrhiza seedlings |
| CN110447471A (en) * | 2019-07-31 | 2019-11-15 | 江南大学 | Plant cultivation device is used in a kind of experiment of blade inoculation endophyte of plant |
| CN110476648A (en) * | 2019-08-02 | 2019-11-22 | 江南大学 | A kind of root-pouring method inoculated plant endophyte experiment sterile culture device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN202949810U (en) | Suspended dendrobium cultivation device | |
| CN105907671B (en) | A kind of Agaricus bisporus endophyte and application thereof | |
| CN104164367A (en) | Dried silkworm cordyceps militaris and culture method thereof | |
| CN103283608B (en) | Factory cultivation strains of needle mushrooms, and cultivation method thereof | |
| CN105557347B (en) | The Seedling Inoculation method of Botrytis cinerea Disease Resistance Identification | |
| CN103858676B (en) | The preparation method of a kind of Cordyceps militaris liquid spawn | |
| CN107460133A (en) | Dark color has every endogenetic fungus HS40 and its application in dendrobium candidum production | |
| CN103493685A (en) | Method for cultivating phellinus igniarius by using wild living tree stumps | |
| CN108315284A (en) | It is a kind of to produce protease, the husky good fortune bacillus of anti-botrytis cinerea and its application | |
| CN107125028A (en) | A kind of wild yellow ring squama agaric domestication and artificial culturing method | |
| CN104560787B (en) | A kind of peanut plant growth-promoting rhizobacteria HS11 and its application | |
| CN106701619A (en) | High-density fermentation method for bacillus amyloliquefaciens and preparation method of microbial agent of bacillus amyloliquefaciens | |
| CN109504611A (en) | One plant of bletilla endogenetic fungus 1-G1 and its application | |
| CN105230342B (en) | Lyophyllum decastes bacterial strain and its cultural method and application | |
| CN108476981A (en) | A kind of screening technique of anti-blight banana germplasm | |
| CN105557312A (en) | Method for cultivating Cordyceps products by taking Tenebrio molitor (L.) larvae as carriers | |
| CN102329739B (en) | Aspergillus versicolor for fermenting Jatropha cake to manufacture biological bacterial manure | |
| CN103981103A (en) | DSE (Dark Septate Endophyte) strain J-N3 and applications thereof in dendrobium candidum production | |
| CN201585281U (en) | Edible fungi cultivating device | |
| CN115927047A (en) | Seed growth promoting microbial reagent and preparation method thereof | |
| CN103444440A (en) | Lucid ganoderma production method | |
| CN103270890A (en) | Method of culturing Taiwanofungus camphoratus by dish | |
| CN103858661A (en) | Semi-artificial cultivation method for boletus edulis | |
| CN109456902A (en) | One plant of bletilla endogenetic fungus 1-N2 and its application | |
| CN104560786A (en) | Maize root growth-promoting bacterium YM6 and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180112 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |