CN103937668B - Nostoc Algal Ecology simulation culture systems and cultural method - Google Patents
Nostoc Algal Ecology simulation culture systems and cultural method Download PDFInfo
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- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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Abstract
The invention provides Nostoc Algal Ecology simulation culture systems and cultural method, this system comprises nutrient solution pond for recovering (7), Multilayer culture shelf (1), multiple illumination apparatus (3), Ethylene recov filtering system (6), at least one shower nozzle (12).The method is placed on Multilayer culture shelf by culture plate with holes, inoculates the algae kind with algae kind to be the diameter obtained by sterile culture be 1mm ~ 2mm, be evenly laid in culture plate with holes during inoculation by algae kind, is the density domination of inoculation 0.5? g/cm
2~ 1g/cm
2, inoculation thickness is 1cm-2cm; Nutrient solution is sprayed, controlled light and culture-liquid temp on culture.The method can economize on water 80%, and in Nostoc culturing process, spheroid not easily breaks, and in culturing process, the anti-assorted algae pollution ability of Nostoc is strong, and assorted algae pollution rate is low, and the dry matter content of the Nostoc of production can up to 8%.
Description
Technical field
The invention belongs to algae culture technical field, specifically belong to culture systems and the cultural method of Nostoc Algal Ecology simulation culture systems and cultural method, particularly Nostoc.
Background technology
Nostoc is galloped along on horseback the rare medicine-food two-purpose Fresh Watcr Blue Algae of one of town special product in Hefeng of Hubei of China, and Yin Dongjin people claims the astrologist that makes pills of immortality of Ge Xian public affairs, physician, Taoism theorist Ge Hongxian and the emperor, and crown prince's body of recovering after eating this thing is for a long time smooth, then bestows the name called " Nostoc ".It and Nostoc commune (Nostoccommunevanch.), deliver vegetables (N.flagelliforme) belong to together and belong to Cyanophyta (Cyanophyta), Cyanophyceae (Cyanophyceae), section grows Cutleriales (Hormogonales), nostocaceae (Nostocaceae), Nostoc (Nostoc), formal name used at school " intends spherical nostoc " (Nostocsphaeroidskuz).Nostoc is a kind of many cells colony, its principal shape is spherical, minority is fusiform, oval row, dumb-bell shape or flats, there is a common colloid clothing sheath outside of its filament, easy formation laminate structure, comparatively robust, in blackish green, wild population diameter can reach 2 centimetres, and the Nostoc diameter of artificial culture can reach 5 centimetres.
Nostoc is China's tradition medicine-food two-purpose fresh water algae, and since the Eastern Jin Dynasty, many famous medical books, recipe have a large amount of record to Nostoc.The medical book such as " Mingyi Bielu ", Compendium of Material Medica, " property of medicine is examined ", " national herbal medicine compilation " thinks that Nostoc has improving eyesight benefit gas, controls nyctalopia, antipyretic clearly every, Ginseng Extract, treatment are scalded and have the effects such as beauty treatment concurrently; Nostoc because its shape and color is attractive in appearance, moisten good to eat and salubrious fragrance, thus to eat in book menu numerous ancient times in " green dish is praised ", " edible wild herbs spectrum ", " adjust Ding Ji ", " Confucian Temple name food " etc. and all record many cooking methods, and clear history is recited as tribute, the food for the emperor, last emperor Pu Yi institute shows in " my first half of one's life " and just bears in mind constantly " duck fourth heats up in a steamer Nostoc " always.On March 2nd, 1980 the Peoples Daily, on September 26th, 1994 the Peoples Daily overseas edition and on October 13rd, 1994 Liberation Daily " newspaper digest " in all have introduction to Nostoc, and to be called " rare treasure ".
Nostoc has high nutritive value, and modern experimental analytical results shows: Nostoc, containing 18 seed amino acids, wherein contains eight seed amino acids of needed by human; In Nostoc dry-matter various component content be total protein up to about 56% (wherein 10% for tool can the Phycocyanins, C-of anti-cancer and cancer-preventing function), total reducing sugar be 24.19%(wherein 8-12% be can the anticancer active polysaccharide of radioprotective), crude fat 8.11%, carbohydrate 12.69%, ash content 10.88%, chlorophyll 30.98mg/g; Xitix 5.5mg/g, Determination of Vitamin C is close to fresh jujube, higher than hawthorn more than 5 times; VITMAIN B1, B2 are higher than general fungus algae; Containing 15 kinds, mineral substance, the abundantest has phosphorus, sulphur, calcium, potassium, iron etc., and less has lead, silicon, magnesium, barium, germanium etc., trace element has zinc, copper, manganese, also have starch and carbohydrate, its calcium content, higher than general vegetables, is a kind of fabulous Natural Rich Calcium nutritive food.Because Nostoc has effect of dietotherapy, health care, nourishing, its nutrition proportion of composing meets human body needs very much, is " best protective foods " by world health organisation recommendations.
There is due to Nostoc the special efficacies such as delicious food, dietotherapy, health care, nourishing, some businessmans have carried out promotion energetically to it in recent years, Nostoc is made to obtain the accreditation of more and more people, market demand is increasing, price is also more and more higher, on market, high-quality fresh goods price is up to 400 yuan/kilogram, and dry product is especially up to 3600 yuan/kilogram.
According to aquatic institute of Chinese Academy of Sciences Li Shanghao academician textual criticism, Nostoc is very harsh to the requirement of growing environment, and growth scope is very narrow, except Hefeng County, Hubei Province is galloped along on horseback except town still has more than 10,000 mu, only has the distribution of minute quantity in Africa.Because its natural distributed is narrow, yield poorly, enjoyed for imperial family and high-level officials mainly as tribute, the food for the emperor in the past.In recent years along with a large amount of uses of agricultural chemicals, chemical fertilizer, add environmental pollution day by day seriously, the growing environment of Nostoc is subject to very big destruction, the field of suitable Nostoc growth is fewer and feweri, output is more and more lower, and its natural annual production is less than five tons, and resource is on the verge of exhaustion.
Many researchists drop into the artificial culture method of a large amount of manpower and materials to Nostoc and are studied in recent years, at present, about the artificial culture method of Nostoc studies have reported that: the links such as cultural method disclosed in Chinese patent literature CN1294190 (applying date: 1999.10.22) comprises nutrient solution preparation, prepared by algae kind, aerated culture, hardening treatment, screening results and drying, culture vessel is glass jar or raceway pond.The links such as method disclosed in Chinese patent literature CN1528127 (applying date: 2003.10.10) comprises nutrient solution preparation, prepared by algae kind, bio-reactor aerated culture, hardening treatment, screening results.Chinese patent literature CN101792716 (applying date: 2010.03.10) adopts semi-enclosed indoor pond to accomplish scale production, and discloses the structural principle in pond and cultivate the optimal conditions of Nostoc 7 fundamentals.Chinese patent literature CN201424471 (applying date: 2009.06.09) discloses a kind of air lift unit for Nostoc cultivation and Nostoc artificial culture device, the high dispersing produced in culturing process is stingy, and bag is spread in nutrient solution, makes Nostoc form regular rise and fall campaign in process of growth.No matter the culture vessel that above patent documentation uses in culturing process is glass jar, bio-reactor, pond or raceway pond, Nostoc is all needed to mix with nutrient solution, Nostoc must be made to be suspended in nutrient solution by ventilation or impeller in whole culturing process, in the culture cycle of nearly 90 days, a nutrient solution need be changed, in order to the physical strength improving spheroid also needs to carry out hardening treatment before results every 7 ~ 10 days; Nostoc is made to be suspended in nutrient solution owing to needing to pass into large quantity of air in culturing process, and the violent surging force of air-flow and make Nostoc spheroid subject to damage with the friction of culture vessel, broken serious, general percentage of damage is between 5%-10%, broken serious more than 10%, even can all break when changing nutrient solution.Chinese patent literature CN1544612 (applying date: 2003.11.25) discloses a kind of method of Control pollution in Nostoc, Nostoc commune culturing process, mainly comprises purebred cultivation, culture vessel sterilization, manual cleaning Nostoc, filtration and changes the steps such as nutrient solution.Because Nostoc is grown in the aqueous solution, its water content is very high, and dry matter content generally only has about 1.5-2%.Above Nostoc is cultivated in Patents, water, electricity and manually expend greatly, and production cost is high.
Summary of the invention
Expend greatly for water power in Nostoc culturing process in prior art, easily broken, be subject to assorted algae pollution, cultivate after the low technical problem of Nostoc dry matter content, the application aims to provide a kind of Nostoc Algal Ecology simulation culture systems and cultural method.This system measures body design according to the fostering requirement of Nostoc, structure is simple, can carry out temperature control to nutrient solution, and recycles, and is suitable for Nostoc algae culture.This cultural method can economize on water more than 80%, brownout more than 50%, makes Nostoc not broken in culturing process, and assorted algae pollution rate is low, and the dry matter content of Nostoc obviously increases.
For achieving the above object, technical scheme of the present invention is:
Nostoc Algal Ecology simulation culture systems, comprise nutrient solution pond for recovering, also comprise by multiple passive vertical support bar and multiple Multilayer culture shelf, multiple illumination apparatus, Ethylene recov filtering system, nutrient solution delivery controller, nutrient solution transport pipe, at least one shower nozzle formed for the horizontal support bar placing culture plate with holes; Described illumination apparatus is arranged on the below of any one deck horizontal support bar of Multilayer culture shelf, is provided with nutrient solution recovering tray bottom described Multilayer culture shelf, and described nutrient solution recovering tray is communicated with nutrient solution pond for recovering by recovery channel, Ethylene recov filtering system; Described nutrient solution pond for recovering is provided with nutrient solution transferpump and temperature control device in being communicated with; Described nutrient solution transferpump is communicated with shower nozzle by nutrient solution transport pipe, and described shower nozzle is positioned at the top of Multilayer culture shelf top layer.
Described nutrient solution transport pipe is preferably provided with nutrient solution delivery controller.
The aperture of described culture plate with holes preferably≤1mm.
The interlamellar spacing of described Multilayer culture shelf is preferably 30cm-40cm.
The distance of described shower nozzle and Multilayer culture shelf top layer is preferably 40cm-50cm.
Nostoc Algal Ecology simulation cultural method, use described culture systems, concrete steps are:
(1) with glasshouse or plastic greenhouse greenhouse for cultivating place, above-mentioned culture systems is placed in and cultivates in place, culture plate with holes is placed on Multilayer culture shelf, the every interlayer of Multilayer culture shelf is divided into 30cm ~ 40cm, culture plate aperture≤1mm with holes, the distance arranging shower nozzle and Multilayer culture shelf top layer is 40cm-50cm;
(2) the inoculation algae kind of algae kind to be the diameter obtained by sterile culture be 1mm ~ 2mm, be evenly laid in culture plate with holes by algae kind during inoculation, the density domination of inoculation is at 0.5g/cm
2~ 1g/cm
2, inoculation thickness is 1cm-2cm;
(3) on culture, nutrient solution is sprayed, spray 10 ~ 30 minutes interval times, 1 ~ 2 minute each spray time length, the adjustment of time is to keep all culture surface wettabilities for standard, nutrient solution is dripped to lower floor by upper strata, finally flow to the nutrient solution catch tray of the lowest layer, then be recovered to nutrient solution pond for recovering, recycle; Meanwhile, controlled light remains between 1000lx ~ 20000lx, controls culture-liquid temp and remains between 20 DEG C ~ 30 DEG C;
Described nutrient solution composition and concentration are:
Described Nostoc algae is Nostoc or Nostoc commune.
The present invention will be further explained below:
Basis of the present invention is: we are by carrying out deep understanding to the growth conditions propagating Nostoc in occurring in nature Wild Nostoc and laboratory artificially, simulation Nostoc self-sow mode in conjunction with modern biotechnology, change the training mode of Nostoc, in culturing process, fresh weight Average weight increasing a day can reach more than 5%.Because the current research to Nostoc ecological simulation production system just starts, so the potentiality improving its output are also very large.
Nostoc is cultivated and is comprised following step: nutrient solution component and preparation, and light and temperature condition controls, algal species cultivation, and inoculation is cultivated, and nutrient solution reclaims and process, results and storage.
A. with glasshouse or plastic greenhouse greenhouse for cultivating place, make full use of natural light temperature resource; Simultaneously arrange can the sunshade net of auto-switch, interpolation is certain in greenhouse artificial lighting equipment for top of greenhouse, makes indoor illumination remain on 1000 ~ 20000lx; Directly control culture-liquid temp by temperature controlling instrumentss such as geothermal source air-conditionings, and by controlling the interval time sprayed culture, culture temperature being remained on 20 ~ 30 DEG C, effectively reducing energy consumption.
B. culturing rack framework is made by aluminium alloy, and every interlayer is divided into 30 ~ 40cm, and according to the illumination particularity of Various Seasonal, spacing distance between regulating course, makes intensity of illumination remain on optimum range.Put nutrient solution catch tray bottom culturing rack, and be connected with nutrient solution recovery channel, to reclaim broth filtrate.Culture plate frame with holes is made by stainless steel or other non-toxic material, bottom is aperture 1mm stainless (steel) wire or non-toxic organic web material, after nostoc sphaeroides is put into culture plate with holes, hierarchal arrangement is on culturing rack, interval spray nutrient solution as required, makes culture keep suitable humidity and temperature.
C. nutrient solution fundamental component and concentration the same.Nutrient solution formula need adjust accordingly at the Different growth phases of Nostoc, and the nutrient solution of recovery need be supplemented according to the loss of nutrition when recycling.
D. inoculate and should be by algae kind the excellent algae kind that the diameter obtained by sterile culture is 1 ~ 2mm, be evenly laid in culture plate with holes by algae kind during inoculation, the density domination of inoculation is at 0.5 ~ 1g/cm
2, carry out certain adjustment with season and illumination power.
E. the shower nozzle being controlled to install at culturing rack top by two-position controller sprays nutrient solution to culture, sprays 10 ~ 30 minutes interval times, and each 1 ~ 2 minute time length of spray, the adjustment of time is to keep all culture surface wettabilities for standard.Nutrient solution is dripped to lower floor by upper strata, finally flow to the nutrient solution catch tray of the lowest layer, then is recovered to nutrient solution recovery system.
F. the nutrient solution reclaimed need filter out impurity and flow into pond for recovering, and then is controlled culture-liquid temp at 20 ~ 25 DEG C by temperature control system in pond for recovering, then is re-used by sprinkling equipment.
The ecological simulation production method of described Nostoc and culture systems, be also applicable to the scale operation of other algae of Nostoc such as Nostoc commune.
Nostoc ecological simulation culture systems provided by the present invention is when carrying out pilot scale culture Nostoc and producing; only nostoc sphaeroides need be put into surrounding is stainless steel frame; in the culture plate with holes that the stainless (steel) wire that bottom is aperture about 1mm is made, be placed on culturing rack and cultivate.
Many researchists drop into the artificial culture method of a large amount of manpower and materials to Nostoc and are studied in recent years, at present, the links such as all artificial culture methods about Nostoc all comprise nutrient solution preparation, prepared by algae kind, aerated culture, hardening treatment, screening results and drying, culture vessel is glass jar or raceway pond.The problems such as it is many that these methods all exist production link, and water loss is large, and energy consumption is high, and spheroid easily breaks, and the low and production cost of dry matter content is high.Therefore we devise brand-new Nostoc ecological simulation culture systems and method, can effectively address these problems.
No matter the culture vessel used is glass jar, bio-reactor, pond or raceway pond, Nostoc is all needed to mix with nutrient solution, Nostoc must be made to be suspended in nutrient solution by ventilation or impeller in whole culturing process, in the culture cycle of nearly 90 days, a nutrient solution need be changed, in order to the physical strength improving spheroid also needs to carry out hardening treatment before results every 7 ~ 10 days.In new culture systems, we simplify step, eliminate ventilation, clean, change the process such as nutrient solution, sclerosis, can save a large amount of manpower, effectively increase work efficiency, and reduce production cost.
In new culture systems, Nostoc rests in culture plate not need to carry out suspension culture to Nostoc by we, thus can economize on water more than 80%; Temperature control neither be undertaken by controlling whole culture space, and only need carry out temperature control to nutrient solution and just can reach the temperature condition making culture remain on suitable growth, thus can energy efficient more than 50%.
In new culture systems, can effectively suppress assorted algae pollution, can decreasing pollution more than 70% compared with traditional method.
In culturing process, Nostoc globules rupture is an insoluble problem always.This mainly makes Nostoc be suspended in nutrient solution owing to needing to pass into large quantity of air in culturing process, and the violent surging force of air-flow and make Nostoc spheroid subject to damage with the friction of culture vessel, broken serious, general percentage of damage is between 5%-10%, broken serious more than 10%, even can all break when changing nutrient solution.And using present method to carry out in the process of cultivating, Nostoc spheroid is damaged few, and breakage rate is close to zero.
Because Nostoc is grown in the aqueous solution in classical culture protocols, its water content is very high, and dry matter content generally only has about 1.5-2%.And can, up to 8%, be 2-5 times of traditional method by its dry matter content of Nostoc that method is cultivated.Because dry matter content is high, thus physical strength is high, does not therefore need to carry out special process of setting in culturing process.
Compared with prior art, advantage of the present invention is:
1, to produce technology and equipment needed for Nostoc simple for Nostoc ecological simulation production system, invests little, and production cost is low and can annual continuous seepage;
When 2, using present method, light, the warm subject range of Nostoc are wider, and temperature is at 4-35 DEG C, and illumination all can grow at 600-12000lx, and photosynthetic efficiency is high;
3, Nostoc dry matter content is than being suspended in cultivate in nutrient solution high 2 ~ 3 times, and dry matter content can reach 7 ~ 8%, is 2 ~ 5 times of other cultural method;
4, Nostoc is mixed, and algae pollution rate is low, good stress resistance, and spheroid does not break;
5, owing to only controlling culture-liquid temp, effectively can reduce energy consumption, save energy reaches more than 50%;
6, more than 80% can be economized on water in culturing process;
7, nutrient solution composition and potential of hydrogen are close to natural water, can be recycled, and direct discharge does not pollute the environment yet.
Accompanying drawing explanation
Fig. 1 culture systems structural representation of the present invention;
Fig. 2 culture plate structure for amplifying schematic diagram with holes of the present invention;
Wherein, 1 is Multilayer culture shelf, and 2 is culture plates with holes, 3 is illumination apparatus, and 4 is nutrient solution recovering tray, and 5 is recovery channels, 6 is Ethylene recov filtering systems, and 7 is nutrient solution pond for recoverings, and 8 is nutrient solution transferpumps, 9 is temperature control devices, 10 is nutrient solution delivery controller, and 11 is nutrient solution transport pipes, and 12 is shower nozzles, 13 is vertical support bars, and 14 is horizontal support bars.
Embodiment
Below in conjunction with embodiment, the present invention is described further.
Embodiment 1
Nostoc Algal Ecology simulation culture systems, comprise nutrient solution pond for recovering 7, also comprise by multiple passive vertical support bar 13 and multiple Multilayer culture shelf 1, multiple illumination apparatus 3, Ethylene recov filtering system 6, nutrient solution delivery controller 10, nutrient solution transport pipe 11, at least one shower nozzle 12 formed for the horizontal support bar 14 placing culture plate 2 with holes; Described illumination apparatus 3 is arranged on the below of any one deck horizontal support bar 14 of Multilayer culture shelf 1, be provided with nutrient solution recovering tray 4 bottom described Multilayer culture shelf 1, described nutrient solution recovering tray 4 is communicated with nutrient solution pond for recovering 7 by recovery channel 5, Ethylene recov filtering system 6; Nutrient solution transferpump 8 and temperature control device 9 is provided with in described nutrient solution pond for recovering 7 is communicated with; Described nutrient solution transferpump 8 is communicated with shower nozzle 12 by nutrient solution transport pipe 11, and described shower nozzle 12 is positioned at the top of Multilayer culture shelf 1 top layer.
Described nutrient solution transport pipe 11 is provided with nutrient solution delivery controller 10.Described culture plate with holes 2 is netted body of stainless steel, aperture≤1mm.The interlamellar spacing of described Multilayer culture shelf 1 is 30cm-40cm.Described shower nozzle 12 is 40cm-50cm with the distance of Multilayer culture shelf 1 top layer.
Embodiment 2
For Nostoc, Nostoc ecological simulation cultural method, cultivate by culture systems described in embodiment 1, specifically comprise:
Cultivate place and equipment: with glasshouse or plastic greenhouse greenhouse for cultivating place, described culture systems is placed in and cultivates in place, culture plate with holes is placed on Multilayer culture shelf, the every interlayer of Multilayer culture shelf is divided into 30cm ~ 40cm, culture plate aperture≤1mm with holes, the distance arranging shower nozzle and Multilayer culture shelf top layer is 40cm-50cm;
Light source: the required light intensity of Nostoc growth is 1000-8000lx, without source of artificial light, when light intensity is too high, can need reduce light intensity by sunshade net when natural light light intensity meets this condition.Ordinary Light Sources Have is water-proof daylight lamp pipe or LED, under these lamps are arranged in the horizontal frame of culturing rack, to maintain the irradiation that frond can obtain 1000-8000lx light intensity.
Temperature: the growth temperature range of Nostoc is 10-35 DEG C, and optimum temps is at about 20-25 DEG C.When natural temperature is in this temperature range, only greenhouse side form need be opened and teleblem carries out ventilation; When temperature is too low or too high, culture need be made to keep the temperature be suitable for interval time by regulating culture-liquid temp and spray.
Nutrient solution composition and concentration are:
Prepared by algae kind: nostoc sphaeroides divides firsts and seconds two kinds.When wherein prepared by First stage algae seeds, first prepare nutrient solution with distilled water, be then dispensed into 50-l000ml Erlenmeyer flask or reagent bottle, through high pressure 121 DEG C of sterilizings 20 minutes after sealing, cool for subsequent use; Then select growth vigorous, shapeliness, good springiness and free of contamination Nostoc spheroid, carry out disinfection under stringent asepsis requirements in the lab, be inoculated into the substratum prepared after homogenate, pass into filtrated air and carry out aerated culture; During cultivation, temperature controls at 20-25 DEG C, and illumination controls at 3000-5000lx, and adds appropriate ruddiness.When frond cultivates diameter 0.5-1mm, be transferred in 10-20L carboy, carry out enlarged culturing, culture condition is the same; When frond diameter grows to about 2mm, check that growth conditions is good, pollution-free and namely can be used as the production kind of secondary algae kind).
Inoculation and cultivation: the culture plate with holes fine quality production kind prepared by above method being put into bottom part aperture diameter 1mm, the density domination of inoculation is at 0.5g/cm
2~ 1g/cm
2inoculation thickness is 1-2cm, then is placed on culturing rack by culture plate layering with holes, is controlled by culture-liquid temp at 20-25 DEG C, in dish, spray nutrient solution by certain time interval, interval time is as the criterion to keep culture surface wettability and to make culture temperature remain on 15-30 DEG C; It should be made to meet culture condition by sunshade or by source of artificial light supplementary light when illumination is crossed strong or not enough.The nutrient solution of spray is successively leaked down to lower floor by upper strata, finally fall into undermost nutrient solution catch tray, collection tube is flowed into again by collector tray bottom outlet, equipment enters nutrient solution collecting tank after filtering after filtration, culture-liquid temp is adjusted to 20-25 DEG C of winter temperature by temperature controlling instruments more slightly high, summer temp is slightly low), then re-used by spray piping.In collecting tank, the concentration of nutrient solution composition must be monitored, and loss part must be supplied in time.In culturing process, every day need stir Nostoc 1-2 time, assigns in two culture plates with holes continue to cultivate when Nostoc increment is double, by that analogy, until Nostoc grows into 6-8mm, and direct marketing or sale after drying in the shade after screen sizing excessively.
By drying rear detection, Product checking: not having rupture event by being observed visually Nostoc spheroid, effectively inhibit assorted algae pollution, decreasing pollution more than 70% compared with traditional method, show that Nostoc dry matter content is 7%-8%.
Claims (3)
1. Nostoc Algal Ecology simulation culture systems, comprise nutrient solution pond for recovering (7), it is characterized in that, also comprising Multilayer culture shelf (1), multiple illumination apparatus (3), Ethylene recov filtering system (6), nutrient solution delivery controller (10), nutrient solution transport pipe (11), at least one shower nozzle (12) of being made up of multiple passive vertical support bar (13) and multiple horizontal support bar (14) for placing culture plate with holes (2); Described illumination apparatus (3) is arranged on the below of any one horizontal support bar (14) of Multilayer culture shelf (1), described Multilayer culture shelf (1) bottom is provided with nutrient solution recovering tray (4), and described nutrient solution recovering tray (4) is communicated with nutrient solution pond for recovering (7) by recovery channel (5), Ethylene recov filtering system (6); Described nutrient solution pond for recovering (7) is provided with nutrient solution transferpump (8) and temperature control device (9) in being communicated with; Described nutrient solution transferpump (8) is communicated with shower nozzle (12) by nutrient solution transport pipe (11), and described shower nozzle (12) is positioned at the top of Multilayer culture shelf (1) top layer; Aperture≤the 1mm of described culture plate with holes (2), and the aperture of described culture plate with holes (2) is less than the diameter of nostoc sphaeroides; The interlamellar spacing of described Multilayer culture shelf (1) is 30cm-40cm, and described shower nozzle (12) is 40cm-50cm with the distance of Multilayer culture shelf (1) top layer; Described Nostoc algae is Nostoc.
2. Nostoc Algal Ecology simulates culture systems according to claim 1, and it is characterized in that, described nutrient solution transport pipe (11) is provided with nutrient solution delivery controller (10).
3. Nostoc Algal Ecology simulation cultural method, is characterized in that, use culture systems described in claim 1 or 2, concrete steps are:
(1) with glasshouse or plastic greenhouse greenhouse for cultivating place, described for one of claim 1-2 culture systems is placed in and cultivates in place, culture plate with holes is placed on Multilayer culture shelf, the every interlayer of Multilayer culture shelf is divided into 30cm ~ 40cm, culture plate aperture≤1mm with holes, the distance arranging shower nozzle and Multilayer culture shelf top layer is 40cm-50cm;
(2) the inoculation algae kind of algae kind to be the diameter obtained by sterile culture be 1mm ~ 2mm, be evenly laid in culture plate with holes by algae kind during inoculation, the density domination of inoculation is at 0.5g/cm
2~ 1g/cm
2, inoculation thickness is 1cm-2cm;
(3) on culture, nutrient solution is sprayed, spray 10 ~ 30 minutes interval times, 1 ~ 2 minute each spray time length, the adjustment of time is to keep all culture surface wettabilities for standard, nutrient solution is dripped to lower floor by upper strata, finally flow to the nutrient solution catch tray of the lowest layer, then be recovered to nutrient solution pond for recovering, recycle; Meanwhile, controlled light remains between 1000lx ~ 20000lx, controls culture-liquid temp and remains between 20 DEG C ~ 30 DEG C;
Described nutrient solution composition and concentration are:
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| CN104178426A (en) * | 2014-08-08 | 2014-12-03 | 新奥科技发展有限公司 | Method and device for controlling culture temperature of microalgae |
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| CN105950462B (en) * | 2016-06-14 | 2018-03-02 | 常州市环境科学研究院 | Bloom blue algae accept a surrender rain shadow sound analogue means |
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| CN108148787A (en) * | 2018-02-07 | 2018-06-12 | 青岛浩然生态海洋水产科技有限公司 | A kind of method of pilot scale culture nostoc |
| CN111363677A (en) * | 2019-04-17 | 2020-07-03 | 张兰英 | Automatic culture system and device for nostoc commune |
| CN110591890A (en) * | 2019-09-12 | 2019-12-20 | 岭南设计集团有限公司 | Curtain wall of photobioreactor |
| CN111264370A (en) * | 2020-01-17 | 2020-06-12 | 中山大学 | Production system and production method for repairing ecological blanket in tailing pond |
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