CN107006362A - A kind of device and store method of Sargassaceae marine alga preserving seed - Google Patents
A kind of device and store method of Sargassaceae marine alga preserving seed Download PDFInfo
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- CN107006362A CN107006362A CN201710286923.1A CN201710286923A CN107006362A CN 107006362 A CN107006362 A CN 107006362A CN 201710286923 A CN201710286923 A CN 201710286923A CN 107006362 A CN107006362 A CN 107006362A
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- seawater
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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- Marine Sciences & Fisheries (AREA)
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- Cultivation Of Plants (AREA)
- Cultivation Of Seaweed (AREA)
Abstract
The invention discloses a kind of device and store method of Sargassaceae marine alga preserving seed, device, including cultivating system, circulated filter system, spray system and illumination system.Technology and equipment needed for the culture systems is simple, invests small, production cost is low, for the live body preserving seed of Sargassaceae marine alga, Study on Physiological and Ecological Characteristics provide it is a kind of can observe at any time, experimental implementation is easy, easy culture apparatus and long-period culture method;When carrying out Sargassaceae algae culture using the preserving seed device, the dry dewiness state under natural habitat is simulated, frond is longer with air contact time, photosynthetic efficiency is high, more utilizes the growth of frond;Marine alga preserving seed is carried out using this method, the miscellaneous algae that grows nonparasitically upon another plant is less, good stress resistance, can preserve for a long time indoors;The inventive method manages convenient during marine alga preserving seed, and water body used is less, and seawater circulation is used, and can economize on water more than 80%.
Description
Technical field
The present invention relates to a kind of tangleweed preserving seed device and store method, and in particular to a kind of Sargassaceae marine alga
The device and store method of preserving seed, belong to algae Germ-plasma resources protection and culture technique field.
Background technology
Sargassaceae marine alga is the tangleweed that a class has higher economic value, in cultivation, medicines and health protection, biological system
The fields such as product, food processing, aquatic feeds, marine alga fertilizer, chemical industry and marine eco-environment protection have important exploitation and
Application prospect.In recent years, due to the pollution and excessive harvesting of inshore, its stock number is caused drastically to decline, fine provenance is deficient
It is weary, seriously constrain the development of whole industry.Therefore, it is industry the germ plasm resource with merit to be carried out into propagation in scale
The critical path of hair is melted, and the preservation of fine germplasm resources is the key for improving scale Seedling production yield and quality.
Sargassaceae marine alga is widely distributed in China coastal seas, is mainly grown in middle damp band and low tide band rock or or stone natural pond
In, there is stronger adaptive faculty to intertidal zone environment, be exposed to afterwards under daylight without influenceing it to grow on the ebb.Existing skill
Art is primarily present following several to the store method mainly air-charging incubation in the controllable tank of light temperature of tangleweed living materials
Individual problem:1st, frond submergence for a long time in the seawater, lacks washing away for ocean current and wave under natural habitat, and high water time water
Raw state gives birth to exchanging for state with the gas of time of low water, and there are some researches show the dry dew stress of low tide is influence Intertidal Algae life
Long and survival key factor;2nd, easily grow nonparasitically upon another plant miscellaneous algae in incubation, and is difficult to remove, therefore Sargassaceae in room conditions
Marine alga can not be long-term survival and growth;3rd, Sargassaceae marine alga frond is larger, and culture is relatively large, it is necessary to strict control with water body
The condition of culture such as temperature processed and illumination, and need often to change seawater, water power and artificial consuming are big.
The live bodies of Sargassaceae marine alga fine germplasm resources is preserved, in the urgent need to a kind of preferable experiment porch, with
In indoor long-term incubation, it is grown and physiological and ecological characteristic carries out long-term follow research.
The content of the invention
The technical problems to be solved by the invention are to be directed to the deficiencies in the prior art, and provide a kind of Sargassaceae
The device and store method of marine alga preserving seed, it can solve prior art to long-term incubation in Sargassaceae marine alga room
Dry dewiness state under the natural habitat of middle shortage intertidal zone, easily by miscellaneous algae pollution, be difficult to indoor culture for a long time, water power and expend big etc.
Problem.
To achieve these goals, the present invention is adopted the following technical scheme that:
A kind of device of Sargassaceae marine alga preserving seed, including cultivating system, circulated filter system, spray system and light
Lighting system, it is characterised in that:
The main body of described cultivating system is that one piece of cultivation Dropbox is fixed with the box for breeding of top end opening, its madial wall;
The Four built in boundaries of described cultivation Dropbox is on cultivation box wall and card is parallel with bottom face;Described cultivation Dropbox
Bottom is additionally provided with vertical support bar, and the bottom face of box for breeding is fixed in the end of support bar;Support bar further keeps supporting
Grow the horizontal support of Dropbox;
Described circulated filter system includes sea water filter box, seawater recovery channel, seawater recovery pond and seawater delivery pipe
Road, wherein:Sea water filter box is placed in the interior bottom of box for breeding, and sea water filter box is connected by seawater recovery channel and seawater recovery pond
It is logical;Seawater delivery pump and temperature control device, the delivery port of seawater delivery pump and entering for sea water conveying pipelines are provided with seawater recovery pond
Hose nozzle is connected;Regulation and control seawater velocity, the seawater delivery controller of flow are provided with sea water conveying pipelines;
Described spray system is multiple shower nozzles, and shower nozzle is connected with the effluent pipe mouth of sea water conveying pipelines, and shower nozzle passes through
Support is fixed on the top of cultivation Dropbox;
Described illumination system is several groups of parallel, in parallel LEDs, and the top of spray system is arranged at by support,
Every group of LED is equipped with a switch timing controller and adjusts switch respectively to control light intensity.
In above-mentioned technical proposal, described box for breeding, its material is lucite material, glass thickness 8mm;Its outside dimension is
Long 80cm × wide 50cm × high 40cm.
In above-mentioned technical proposal, described cultivation Dropbox is the polythene net that aperture is 6mm.
In above-mentioned technical proposal, described sea water filter box is placed with filter cotton and active charcoal bag in box, miscellaneous for filtering
Thing.
In above-mentioned technical proposal, described temperature control device includes temp probe and temperature digital panel, temp probe detection
The temperature of seawater recovery pond maritime interior waters, temperature digital panel setting alarm temperature, seawater is reached after setting alarm temperature, is opened defeated
Pump and delivery controller are sent, then the spray system connected by conveyance conduit sprays.
In above-mentioned technical proposal, described shower nozzle, controllable water spray speed and uninterrupted, its material are stainless steel or brass
Material, jet diameters 250mm, shower nozzle distance cultivation Dropbox 30-40cm.
In above-mentioned technical proposal, described illumination system is 6 groups of parallel, in parallel LEDs, and the length of LED is 0.9
Rice.
The present invention also provides a kind of store method of Sargassaceae marine alga germplasm, comprises the following steps:
(1) appropriate seawater is put into seawater recovery pond, temperature control device is opened, setting ocean temperature is marine alga indoor growing
Optimum temperature;Reach after design temperature, open seawater delivery pump and delivery controller, seawater is flowed into by sea water conveying pipelines to be sprayed
In head, it is set to keep moistening so as to spray cultivation Dropbox;Meanwhile, illumination system control intensity of illumination is opened, and it is fixed to adjust switch
When controller control the periodicity of illumination of illumination system;
(2) Sargassaceae marine alga of the selection with merit, 5-6 centimetres of clip frond major branch is careful with sterilizing seawater
After cleaning, cleared surface attachments, uniformly it is fixed in culture Dropbox;
(3) seawater delivery controller is adjusted, so that interval and the flow of adjusting shower nozzle sprayed sea water, keep all frond tables
Face is moistened, and the seawater of sprinkling is recycled;Sprayed sea water interval time and resistance to dry dew characteristic of the duration according to marine alga
And adjust;
(4) seawater reclaimed first passes through sea water filter box and filtered out after impurity again through the inflow seawater recovery of seawater recovery channel
Chi Zhong, then again in seawater recovery pond by temperature control device by ocean temperature control marine alga indoor growing optimum temperature,
Re-used again by seawater delivery pump into sprinkling equipment;
(5) regular maintenance:The seawater of recovery needs periodic monitoring salinity when being recycled, and is mended according to loss
Fill and change;The debris such as regular algae removed in box for breeding;The growing state of routine observation culture frond, branch length to 10
Centimetre when, remove base portion, retain 5-6 centimetres of frond tip, be further continued for being fixed in culture Dropbox and carry out live body preserving seed training
Support.
In above-mentioned technical proposal, in step (1), the intensity of illumination for controlling illumination system is 3000~4000lx, controls light
It is illumination according to the cycle:Dark=12:12h.
In above-mentioned technical proposal, in step (1), the optimum temperature of marine alga indoor growing is 12-18 DEG C, preferably 14-16
℃。
In above-mentioned technical proposal, in step (2), Sargassaceae marine alga be fixed on culture Dropbox on density domination every
10cm2For 2~3 plants.
Technical solution of the present invention has advantages below:
1st, the technology and equipment needed for the culture systems is simple, invests small, production cost is low, is the work of Sargassaceae marine alga
Body preserving seed, Study on Physiological and Ecological Characteristics provide it is a kind of can observe at any time, experimental implementation is easy, easy culture apparatus and
Long-period culture method;
When the 2nd, carrying out Sargassaceae algae culture using the preserving seed device, the dry dewiness under natural habitat is simulated
State, frond is longer with air contact time, and photosynthetic efficiency is high, more utilizes the growth of frond;
3rd, marine alga preserving seed is carried out using this method, the miscellaneous algae that grows nonparasitically upon another plant is less, good stress resistance, can preserve for a long time indoors;
4th, the inventive method manages convenient during marine alga preserving seed, and water body used is less, and seawater circulation is used,
It can economize on water more than 80%.
Brief description of the drawings
The overall structure diagram of the device of Fig. 1 Sargassaceae marine alga preserving seeds of the present invention;
The enlarged diagram of the cultivation Dropbox of the device of Fig. 2 Sargassaceae marine alga preserving seeds of the present invention;
Wherein:1 is box for breeding, and 2 be cultivation Dropbox, and 3 be support bar, and 4 be sea water filter box, and 5 be seawater recovery channel, 6
It is seawater recovery pond, 7 be seawater delivery pump, and 8 be temperature control device, and 9 be seawater delivery controller, and 10 be sea water conveying pipelines, 11
It is shower nozzle, 12 be LED, and 13 be switch timing controller.
Embodiment
The embodiment to technical solution of the present invention is described in detail below, but the present invention is not limited in following description
Hold:
A kind of device of Sargassaceae marine alga preserving seed, including cultivating system, circulated filter system, spray system and light
Lighting system, as illustrated in fig. 1 and 2:
The main body of described cultivating system is that one piece of cultivation Dropbox is fixed with the box for breeding 1 of top end opening, its madial wall
2;The Four built in boundaries of described cultivation Dropbox is on cultivation box wall and card is parallel with bottom face;Described cultivation Dropbox
Bottom be additionally provided with vertical support bar 3, the bottom face of box for breeding is fixed in the end of support bar;Support bar is further kept
Cultivate the horizontal support of Dropbox;Box for breeding, its material is lucite material, glass thickness 8mm;Its outside dimension is long 80cm × wide
50cm × high 40cm;Dropbox is cultivated, is the polythene net that aperture is 6mm.
Described circulated filter system includes sea water filter box 4, seawater recovery channel 5, seawater recovery pond 6 and seawater conveying
Pipeline 10, wherein:Filter cotton and active charcoal bag are placed with sea water filter box, the interior bottom of box for breeding is placed in;Sea water filter box passes through
Seawater recovery channel is connected with seawater recovery pond;Seawater delivery pump 7 and temperature control device 8, seawater conveying are provided with seawater recovery pond
The delivery port of pump is connected with the water inlet pipe mouth of sea water conveying pipelines;Regulation and control seawater velocity, stream are provided with sea water conveying pipelines
The seawater delivery controller 9 of amount;Temperature control device includes temp probe and temperature digital panel, temp probe detection seawater recovery pond
The temperature of maritime interior waters, temperature digital panel setting alarm temperature.
Described spray system is multiple shower nozzles 11, and shower nozzle is connected with the effluent pipe mouth of sea water conveying pipelines, and shower nozzle leads to
Cross support and be fixed on the top of cultivation Dropbox, distance cultivation Dropbox 30-40cm;The controllable water spray speed of shower nozzle and uninterrupted, its
Material is stainless steel or brass material, jet diameters 250mm.
Described illumination system is 6 groups of parallel, in parallel LEDs 12, and the top of spray system is arranged at by support,
Every group of LED is equipped with a switch timing controller 13 and adjusts switch respectively to control light intensity;The length of LED is 0.9
Rice.
The present invention also provides a kind of store method of Sargassaceae marine alga germplasm, comprises the following steps:
(1) appropriate seawater is put into seawater recovery pond 6, temperature control device 8 is opened, it is raw in marine alga room to set ocean temperature
Long 12-18 DEG C of optimum temperature (being preferably 14-16 DEG C);Reach after design temperature, open seawater delivery pump (7) and conveying control
Device 9, seawater is flowed into shower nozzle by sea water conveying pipelines, it is kept moistening so as to spray cultivation Dropbox;Meanwhile, open illumination system
System control intensity of illumination to be 3000~4000lx, and adjusts and switch timing controller 13 to control the periodicity of illumination of illumination system to be
Illumination:Dark=12:12h;
(2) Sargassaceae marine alga of the selection with merit, 5-6 centimetres of clip frond major branch is careful with sterilizing seawater
After cleaning, cleared surface attachments, uniformly it is fixed in culture Dropbox, density domination is in every 10cm2For 2~3 plants;
(3) seawater delivery controller is adjusted, so that interval and the flow of adjusting shower nozzle sprayed sea water, keep all frond tables
Face is moistened, and the seawater of sprinkling is recycled;Sprayed sea water interval time and resistance to dry dew characteristic of the duration according to marine alga
And adjust;
(4) seawater reclaimed first passes through sea water filter box 4 and filtered out after impurity again through the inflow seawater time of seawater recovery channel 5
In receives pond 6, the most thermophilic in marine alga indoor growing is then controlled ocean temperature by temperature control device 8 in seawater recovery pond again
Degree, then re-used by seawater delivery pump into sprinkling equipment;
(5) regular maintenance:The seawater of recovery needs periodic monitoring salinity when being recycled, and is mended according to loss
Fill and change;The debris such as regular algae removed in box for breeding;The growing state of routine observation culture frond, branch length to 10
Centimetre when, remove base portion, retain 5-6 centimetres of frond tip, be further continued for being fixed in culture Dropbox and carry out live body preserving seed training
Support.
The present invention is expanded on further with reference to specific embodiment:
Embodiment 1:The preservation of sargassum thunbergii germ plasm resource
(1) instrument is opened, appropriate seawater will be put into seawater recovery pond 6, temperature control system is opened, is maintained at ocean temperature
16℃;Seawater delivery pump 7 and delivery controller 9 are opened, spray cultivation Dropbox 2 keeps its moistening;Meanwhile, open illumination system
LED 12 controls illumination to be 4000lx, and adjusts switch timing controller 13 to control the periodicity of illumination to be:Illumination:Dark=12:
12h。
(2) the good sargassum thunbergii of growth selection, by the algae strain major branch alcohol-pickled 30s of volumetric concentration 75%, with a large amount of
Sterilize seawater Rapid Cleaning 3~4 times;The major branch of algae strain is cut, 5~6 centimetres of piece segment length, carefully cleaned, removed with sterilizing seawater
After net surface attachments, uniformly it is fixed in cultivation Dropbox 2, density domination is in every 10cm22~3 plants.
(3) regulation seawater delivery controller 9,1~2 hour sprayed sea water interval time, each spray duration 10~
12 hours, the seawater of sprinkling most exited into seawater recovery system through bottom pipe afterwards.
(4) seawater reclaimed needs first to filter out impurity again through the inflow seawater recovery of seawater recovery channel 5 through sea water filter box 4
Pond 6, is then again controlled ocean temperature at 16 DEG C by temperature control system 8 in seawater recovery pond 6, then pass through seawater delivery pump 7
Re-used into sprinkling equipment.
(5) regular maintenance:
Salinity:A seawater salinity is surveyed weekly, if salinity is higher than 35 ‰, running water or deionized water can be added to adjust salinity,
Place it in 28~32 ‰;
Seawater is changed:Per two weeks changes in seawater recovery pond 1/3 seawater.
Box for breeding is cleared up:The dirt adhered in a box for breeding Nell wall and culture Dropbox and algae, cultivation are removed weekly
Dropbox monthly removes scrub.
(6) growing state of routine observation culture sargassum thunbergii, when branch length is to 10 centimetres, removes base portion, retains frond
Sophisticated 5-6 centimetres, it is further continued for being fixed to preservation in cultivation Dropbox.
(7) sargassum thunbergii elite germplasm is preserved using device embodiment and the present embodiment, frond grows in eight months
In order, survival rate is higher, up to more than 80%.
Embodiment 2:The preservation of sargassum kjellmanianum Yendo germ plasm resource
(1) instrument is opened, appropriate sterilization seawater will be put into seawater recovery pond 6, temperature control system 8 is opened, makes ocean temperature
It is maintained at 14 DEG C;Seawater delivery pump 7 and delivery controller 9 are opened, spray cultivation Dropbox keeps it to moisten;Meanwhile, open illumination
System led lamp 12 controls illumination to be 3000lx, and adjusts timer 13 to control the periodicity of illumination to be:Illumination:Dark=12:12h.
(2) the sargassum kjellmanianum Yendo improved seeds that growth selection gesture is vigorous, algin content is high, surface is removed with hairbrush and tweezers
Debris and aufwuch, by alcohol-pickled 30s of the algae strain major branch with volumetric concentration 75%, with a large amount of sterilization seawater Rapid Cleanings 3
~4 times;The major branch of algae strain is cut, 5~6 centimetres of piece segment length, be uniformly fixed in cultivation Dropbox 2, density domination is in every 10cm2
2~3 plants.
(3) regulation seawater delivery controller 9,5 minutes sprayed sea water interval times, 30 minutes duration are sprayed every time,
The regulation of time is to keep all frond surface wettabilities as standard;The seawater of sprinkling most exits into seawater through bottom pipe afterwards and returned
Receipts system.
(4) seawater reclaimed needs first to filter out impurity again through the inflow seawater recovery of seawater recovery channel 5 through sea water filter box 4
Pond 6, is then again controlled ocean temperature at 14 DEG C by temperature control system 8 in seawater recovery pond 6, then pass through seawater delivery pump 7
Re-used into sprinkling equipment.
(5) regular maintenance:
Salinity:A seawater salinity is surveyed weekly, if salinity is higher than 37 ‰, plus the deionized water of sterilization adjusts salinity, makes it
It is placed in 28~35 ‰;
Seawater is changed:Per two weeks changes in seawater recovery pond 1/3 seawater.
Box for breeding is cleared up:The dirt adhered in a box for breeding Nell wall and culture Dropbox and algae, cultivation are removed weekly
Dropbox monthly removes scrub.
(6) growing state of routine observation culture sargassum kjellmanianum Yendo, when branch length is to 10 centimetres, removes base portion, retains frond
Sophisticated 5-6 centimetres, it is further continued for being fixed to preserving in culture Dropbox and cultivates.
(7) preserved using device embodiment and the present embodiment the sargassum kjellmanianum Yendo improved seeds high to algin content, eight
It is in good condition in individual month, reach the purpose that fine germplasm resources are preserved.
Examples detailed above is technical concept and technical characterstic to illustrate the invention, can not limit the present invention's with this
Protection domain.Equivalent transformation or modification that all essence according to the present invention is done, should all cover in protection scope of the present invention
Within.
Claims (10)
1. a kind of device of Sargassaceae marine alga preserving seed, including cultivating system, circulated filter system, spray system and illumination
System, it is characterised in that:
The main body of described cultivating system is that one piece of cultivation Dropbox is fixed with the box for breeding (1) of top end opening, its madial wall
(2);The Four built in boundaries of described cultivation Dropbox is on cultivation box wall and card is parallel with bottom face;Described culturing net
The bottom of disk is additionally provided with vertical support bar (3), and the bottom face of box for breeding is fixed in the end of support bar;
It is defeated that described circulated filter system includes sea water filter box (4), seawater recovery channel (5), seawater recovery pond (6) and seawater
Pipeline (10) is sent, wherein:Sea water filter box is placed in the interior bottom of box for breeding, and sea water filter box is returned by seawater recovery channel and seawater
Receives pond is connected;Seawater delivery pump (7) and temperature control device (8), the delivery port and seawater of seawater delivery pump are provided with seawater recovery pond
The water inlet pipe mouth of conveyance conduit is connected;The seawater conveying control of regulation and control seawater velocity, flow is provided with sea water conveying pipelines
Device (9);
Described spray system is multiple shower nozzles (11), and shower nozzle is connected with the effluent pipe mouth of sea water conveying pipelines, and shower nozzle passes through
Support is fixed on the top of cultivation Dropbox;
Described illumination system is several groups of parallel, in parallel LEDs (12), and the top of spray system is arranged at by support,
Every group of LED (12) is equipped with a switch timing controller (13), and regulation switchs to control light intensity respectively.
2. device according to claim 1, it is characterised in that:Described box for breeding (1), its material is lucite material
Matter, glass thickness 8mm;Its outside dimension is long 80cm × wide 50cm × high 40cm.
3. device according to claim 1, it is characterised in that:Described cultivation Dropbox (2), is the poly- second that aperture is 6mm
Alkene net.
4. device according to claim 1, it is characterised in that:Be placed with described sea water filter box (4), box filter cotton and
Active charcoal bag, for filtering debris.
5. device according to claim 1, it is characterised in that:Described temperature control device (8) includes temp probe and temperature
Digitizing tablet, temp probe detects the temperature of seawater recovery pond maritime interior waters, temperature digital panel setting alarm temperature.
6. device according to claim 1, it is characterised in that:Described shower nozzle (11), its material is stainless steel or brass
Material, jet diameters 250mm, shower nozzle distance cultivation Dropbox 30-40cm.
7. device according to claim 1, it is characterised in that:Described illumination system is 6 groups of parallel, in parallel LED
Lamp (12), the length of LED is 0.9 meter.
8. a kind of store method of Sargassaceae marine alga germplasm, it is characterised in that comprise the following steps:
(1) appropriate seawater is put into seawater recovery pond (6), temperature control device (8) is opened, it is raw in marine alga room to set ocean temperature
Long optimum temperature;Reach after design temperature, open seawater delivery pump (7) and delivery controller (9), seawater is by seawater delivery pipe
Road is flowed into shower nozzle, it is kept moistening so as to spray cultivation Dropbox;Meanwhile, illumination system control intensity of illumination is opened, and adjust
Section switchs timing controller (13) to control the periodicity of illumination of illumination system;
(2) Sargassaceae marine alga of the selection with merit, 5-6 centimetres of clip frond major branch is carefully cleaned with sterilizing seawater,
After cleared surface attachments, uniformly it is fixed in culture Dropbox;
(3) seawater delivery controller is adjusted, so that interval and the flow of adjusting shower nozzle sprayed sea water, keep all frond surfaces wet
Profit, the seawater of sprinkling is recycled;
(4) seawater reclaimed first passes through sea water filter box (4) and filtered out after impurity again through seawater recovery channel (5) inflow seawater time
In receives pond (6), then ocean temperature is controlled in marine alga indoor growing most by temperature control device (8) in seawater recovery pond again
Thermophilic degree, then re-used by seawater delivery pump into sprinkling equipment;
(5) regular maintenance:The seawater of recovery needs periodic monitoring salinity when being recycled, and supplemented according to loss and
Change;The debris such as regular algae removed in box for breeding;The growing state of routine observation culture frond, branch length is to 10 centimetres
When, remove base portion, retain 5-6 centimetres of frond tip, be further continued for being fixed to progress live body preserving seed culture in culture Dropbox.
9. method according to claim 8, it is characterised in that in step (1), the intensity of illumination for controlling illumination system is
3000~4000lx, it is illumination to control periodicity of illumination:Dark=12:12h;The optimum temperature of marine alga indoor growing is 12-18 DEG C,
Preferably 14-16 DEG C.
10. method according to claim 8, it is characterised in that in step (2), Sargassaceae marine alga is fixed on culture net
Density domination on disk is in every 10cm2For 2~3 plants.
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CN110036903A (en) * | 2019-06-05 | 2019-07-23 | 山东省海洋生物研究院 | A kind of Sargassaceae seaweed scale breeding facility and its application method |
CN111081129A (en) * | 2019-12-31 | 2020-04-28 | 河南科技大学 | Subsurface flow layer ecological simulation experiment device and simulation method |
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CN117136833A (en) * | 2023-08-18 | 2023-12-01 | 生态环境部华南环境科学研究所(生态环境部生态环境应急研究所) | Sargassum periwinkle germplasm preservation method |
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