CN103937668A - Nostoc algae ecological-simulation culturing system and culturing method - Google Patents
Nostoc algae ecological-simulation culturing system and culturing method Download PDFInfo
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention provides a nostoc algae ecological-simulation culturing system and a culturing method. The culturing system comprises a culturing solution recovering tank (7), a multi-layer culturing shelf (1), multiple light irradiation device (3), a recovered solution filtering system (6) and at least one spray nozzle (12). The culturing method comprises the following steps: putting a culturing tray with holes on the multi-layer culturing shelf; when inoculating, uniformly spreading algae seeds in the culturing tray with holes, wherein the inoculation algae seeds obtained by aseptic culturing are the algae seeds with the diameter of 1-2mm, the inoculation density is controlled to be 0.5-1g/cm<2> and the inoculation thickness is 1-2cm; spraying a culturing solution on cultures, and controlling light irradiation and culturing temperature. According to the method, 80% of water can be saved, spheres do not rupture easily in the process of culturing nostoc commune, the wild algae pollution resisting capability of the nostoc commune is high, the rate of wild algae pollution is low, and the content of dry matter in the produced nostoc commune can be up to 8%.
Description
Technical field
The invention belongs to algae culture technical field, specifically belong to culture systems and the cultural method of Nostoc Algal Ecology simulation culture systems and cultural method, particularly Nostoc.
Background technology
Nostoc is the gallop along on horseback a kind of rare medicine-food two-purpose Fresh Watcr Blue Algae of town special product of China Hefeng of Hubei, and Yin Dongjin people claims the astrologist that makes pills of immortality, physician, Taoism theorist Ge Hongxian and the emperor of Ge Xian public affairs, and it is smooth that crown prince eats the body of recovering after this thing for a long time, then bestows the name called " Nostoc ".It and Nostoc commune (Nostoc commune vanch.), deliver vegetables (N.flagelliforme) belong to together and belong to Cyanophyta (Cyanophyta), Cyanophyceae (Cyanophyceae), section is grown Cutleriales (Hormogonales), nostocaceae (Nostocaceae), Nostoc (Nostoc), formal name used at school " is intended spherical nostoc " (Nostoc sphaeroids kuz).Nostoc is a kind of many cells colonies, it is mainly shaped as spherical, minority is fusiform, oval row, dumb-bell shape or flats, there is a common colloid clothing sheath outside of its filament, easily form laminate structure, comparatively robust, is blackish green, wild population diameter can reach 2 centimetres, and the Nostoc diameter of artificial culture can reach 5 centimetres.
Nostoc is China's tradition medicine-food two-purpose fresh water algae, and since the Eastern Jin Dynasty, many famous medical books, recipe have a large amount of records to Nostoc.< < Mingyi Bielu > >, < < Compendium of Materia Medica > >, the < < property of medicine is examined > >, the medical books such as < < whole nation herbal medicine compilation > > think that Nostoc has improving eyesight benefit gas, control nyctalopia, antipyretic clearly every, Ginseng Extract, the effects such as beauty treatment are scalded and are had concurrently in treatment, Nostoc is because its shape and color is attractive in appearance, moisten good to eat and clearly fragrant, thereby praise > > at the green dish of < <, < < edible wild herbs spectrum > >, < < adjusts ancient cooking vessel collection > >, eat numerous ancient times such as < < Confucian Temple name food > > and in book menu, all record many cooking methods, and clear history is recited as tribute, the food for the emperor, last emperor Pu Yi shows < < in > >, " duck fourth is heated up in a steamer Nostoc " just born in mind constantly always my first half of one's life.On March 2nd, 1980 the > > of < < People's Daily, on September 26th, 1994 < < People's Daily > > overseas edition and in October, 1994 13 Liberation Daily < < newspaper digest > > in Nostoc is all had to introduction, and be called " rare treasure ".
Nostoc has high nutritive value, and modern experiment analysis results shows: Nostoc, containing 18 seed amino acids, wherein contains eight seed amino acids of needed by human; In Nostoc dry-matter various component contents be total protein up to 56% left and right (wherein 10% for tool can anti-cancer and cancer-preventing function Phycocyanins, C-), total reducing sugar be 24.19%(wherein 8-12% for can the anticancer active polysaccharide of radioprotective), crude fat 8.11%, carbohydrate 12.69%, ash content 10.88%, chlorophyll 30.98mg/g; Xitix 5.5mg/g, Determination of Vitamin C approaches fresh jujube, higher more than 5 times than hawthorn; VITMAIN B1, B2 are higher than general fungus algae; Containing 15 kinds, mineral substance, the abundantest have phosphorus, sulphur, calcium, potassium, an iron etc., and less have lead, silicon, magnesium, barium, a germanium etc., trace element has zinc, copper, manganese, also have starch and carbohydrate, its calcium content, higher than general vegetables, is a kind of fabulous Natural Rich Calcium nutritive food.Because Nostoc has the effect of dietotherapy, health care, nourishing, its nutrition proportion of composing meets human body needs very much, is " best protective foods " by world health organisation recommendations.
Due to Nostoc, there is the special efficacies such as delicious food, dietotherapy, health care, nourishing, some businessmans have carried out promotion energetically to it in recent years, make Nostoc obtain more and more people's approval, market demand is increasing, price is also more and more higher, on market, high-quality fresh goods price is up to 400 yuan/kilogram, and dry product is especially up to 3600 yuan/kilogram.
According to the Li Shanghao academician of aquatic institute of Chinese Academy of Sciences textual criticism, Nostoc is very harsh to the requirement of growing environment, and growth scope is very narrow, except the town of galloping along on horseback, Hefeng County, Hubei Province still has more than 10,000 mu, only in Africa, has the distribution of minute quantity.Because its natural distributed is narrow, yield poorly, mainly as tribute, the food for the emperor, for imperial family and high-level officials, enjoyed in the past.In recent years along with a large amount of uses of agricultural chemicals, chemical fertilizer, add environmental pollution day by day serious, the growing environment of Nostoc is subject to very big destruction, the field of suitable Nostoc growth is fewer and feweri, output is more and more lower, five tons of its natural annual production less thaies, and resource has been on the verge of exhaustion.
Many researchists drop into a large amount of manpower and materials the artificial culture method of Nostoc are studied in recent years, at present, the artificial culture method of relevant Nostoc studies have reported that: the Chinese patent literature CN1294190 (applying date: 1999.10.22), disclosed cultural method comprises the links such as prepared by nutrient solution preparation, algae kind, aerated culture, hardening treatment, screening is gathered in the crops and be dried, and culture vessel is glass jar or raceway pond.Chinese patent literature CN1528127 (the applying date: 2003.10.10), disclosed method comprises the links such as prepared by nutrient solution preparation, algae kind, bio-reactor aerated culture, hardening treatment, screening results.Chinese patent literature CN101792716 (the applying date: 2010.03.10) adopt semi-enclosed indoor pond to accomplish scale production, and disclose the structural principle in pond and the optimal conditions of cultivating 7 fundamentals of Nostoc.Chinese patent literature CN201424471 (the applying date: 2009.06.09) disclose a kind of air lift unit and Nostoc artificial culture device of cultivating for Nostoc, the high dispersing that produces in culturing process is stingy, and bag is spread in nutrient solution, makes Nostoc form regular rise and fall campaign in process of growth.No matter the culture vessel that above patent documentation is used in culturing process is glass jar, bio-reactor, pond or raceway pond, all need Nostoc to mix with nutrient solution, in whole culturing process, must Nostoc be suspended in nutrient solution by ventilation or impeller, in the culture cycle of nearly 90 days, every 7～10 days, need to change a nutrient solution, before results, in order to improve the physical strength of spheroid, also need to carry out hardening treatment; Owing to needing to pass into large quantity of air in culturing process, Nostoc is suspended in nutrient solution, and the violent surging force of air-flow and make Nostoc spheroid subject to damage with the friction of culture vessel, broken serious, general percentage of damage is between 5%-10%, broken serious in more than 10%, when changing nutrient solution, even can all break.Chinese patent literature CN1544612 (the applying date: 2003.11.25) disclose a kind of method of polluting of controlling in Nostoc, Nostoc commune culturing process, mainly comprised purebred cultivation, culture vessel sterilization, manual cleaning Nostoc, filtration and change the steps such as nutrient solution.Because Nostoc grows in the aqueous solution, its water content is very high, and dry matter content generally only has 1.5-2% left and right.Above Nostoc is cultivated in Patents, water, electricity and manually expend greatly, and production cost is high.
Summary of the invention
For water power in Nostoc culturing process in prior art expend greatly, easily broken, be subject to assorted algae pollution, the low technical problem of Nostoc dry matter content after cultivating, the application aims to provide a kind of Nostoc Algal Ecology simulation culture systems and cultural method.This system is measured body design according to the fostering requirement of Nostoc, simple in structure, can carry out temperature control to nutrient solution, and recycles, and is suitable for Nostoc algae culture.This cultural method can be economized on water more than 80%, and brownout more than 50%, makes Nostoc not broken in culturing process, and assorted algae pollution rate is low, and the dry matter content of Nostoc obviously increases.
For achieving the above object, technical scheme of the present invention is:
Nostoc Algal Ecology simulation culture systems, comprise nutrient solution pond for recovering, also comprise by a plurality of passive vertical support bars and a plurality of Multilayer culture shelf, a plurality of illumination apparatus, recovery liquid filtering system, nutrient solution delivery controller, nutrient solution transport pipe, at least one shower nozzle forming for placing the horizontal support bar of culture plate with holes; Described illumination apparatus is arranged on the below of any one deck horizontal support bar of Multilayer culture shelf, and described Multilayer culture shelf bottom is provided with nutrient solution recovering tray, and described nutrient solution recovering tray is communicated with nutrient solution pond for recovering by recovery channel, recovery liquid filtering system; Described nutrient solution pond for recovering is provided with nutrient solution transferpump and temperature control device in being communicated with; Described nutrient solution transferpump is communicated with shower nozzle by nutrient solution transport pipe, and described shower nozzle is positioned at the top of Multilayer culture shelf top layer.
Described nutrient solution transport pipe is preferably provided with nutrient solution delivery controller.
The aperture of described culture plate with holes preferably≤1mm.
The interlamellar spacing of described Multilayer culture shelf is preferably 30cm-40cm.
The distance of described shower nozzle and Multilayer culture shelf top layer is preferably 40cm-50cm.
Nostoc Algal Ecology simulation cultural method, is used described culture systems, and concrete steps are:
(1) take glasshouse or plastic greenhouse greenhouse as cultivating place, above-mentioned culture systems is placed in and is cultivated in place, culture plate with holes is placed on Multilayer culture shelf, the every interlayer of Multilayer culture shelf is divided into 30cm～40cm, culture plate aperture≤1mm with holes, the distance that shower nozzle and Multilayer culture shelf top layer are set is 40cm-50cm;
(2) inoculation, by algae kind for the algae kind that the diameter that obtains by sterile culture is 1mm～2mm, is evenly laid in algae kind in culture plate with holes during inoculation, and the density of inoculation is controlled at 0.5g/cm
2～1g/cm
2, inoculation thickness is 1cm-2cm;
(3) on culture, spray nutrient solution, spray 10～30 minutes interval times, each 1～2 minute time length of spray, the adjusting of time take that to keep all culture surface wettabilities be standard, nutrient solution is dripped to lower floor by upper strata, finally flow to the nutrient solution catch tray of the lowest layer, then be recovered to nutrient solution pond for recovering, recycle; Meanwhile, controlled light remains between 1000lx～20000lx, controls culture-liquid temp and remains between 20 ℃～30 ℃;
Described nutrient solution composition and concentration are:
Described Nostoc algae is Nostoc or Nostoc commune.
Below the present invention will be further explained:
Basis of the present invention is: we are by carrying out deep understanding to propagating the growth conditions of Nostoc in occurring in nature Wild Nostoc and laboratory artificially, simulation Nostoc self-sow mode in conjunction with modern biotechnology, the training mode that changes Nostoc, in culturing process, fresh weight Average weight increasing a day can reach more than 5%.Owing at present the research of Nostoc ecological simulation production system just being started, so it is also very large to improve the potentiality of its output.
Nostoc is cultivated and is comprised following step: nutrient solution component and preparation, and light and temperature condition is controlled, and algal species cultivation is inoculated, and cultivates, and nutrient solution reclaims and processes, results and storage.
A. take glasshouse or plastic greenhouse greenhouse as cultivating place, make full use of natural light temperature resource; Simultaneously top, greenhouse arrange can auto-switch sunshade net, greenhouse in the certain artificial lighting equipment of interpolation, make indoor illumination remain on 1000～20000lx; By temperature controlling instrumentss such as geothermal source air-conditionings, directly control culture-liquid temp, and by controlling the interval time that culture is sprayed, culture temperature is remained on to 20～30 ℃, effectively reduce energy consumption.
B. culturing rack framework is made by aluminium alloy, and every interlayer is divided into 30～40cm, and according to the illumination particularity of Various Seasonal, spacing distance between regulating course, makes intensity of illumination remain on optimum range.Nutrient solution catch tray is put in culturing rack bottom, and is connected with nutrient solution recovery channel, to reclaim filtration nutrient solution.Culture plate frame with holes by stainless steel or other non-toxic material make, bottom is aperture 1mm stainless (steel) wire or non-toxic organic web material, after nostoc sphaeroides is put into culture plate with holes, hierarchal arrangement is on culturing rack, interval spray nutrient solution, makes culture keep suitable humidity and temperature as required.
C. the basic composition of nutrient solution and concentration are the same.Nutrient solution formula need adjust accordingly at the different growth phases of Nostoc, and the nutrient solution of recovery need be supplemented according to the loss of nutrition when recycling.
D. inoculate by algae kind and should be the good algae kind that the diameter that obtains by sterile culture is 1～2mm, during inoculation, algae kind is evenly laid in culture plate with holes, the density of inoculation is controlled at 0.5～1g/cm
2, with season and illumination power, carry out certain adjustment.
E. by two-position controller, control the shower nozzle of installing at culturing rack top and spray nutrient solution to culture, 10～30 minutes interval times of spray, spray 1～2 minute time length at every turn, the adjusting of time take that to keep all culture surface wettabilities be standard.Nutrient solution is dripped to lower floor by upper strata, finally flow to the nutrient solution catch tray of the lowest layer, then is recovered to nutrient solution recovery system.
F. the nutrient solution reclaiming need filter out impurity and flow into pond for recovering, and then by temperature control system, culture-liquid temp is controlled to 20～25 ℃ in pond for recovering, then re-uses by sprinkling equipment.
Ecological simulation production method and the culture systems of described Nostoc, be also applicable to the scale operation of other algae of Nostoc such as Nostoc commune.
Nostoc ecological simulation culture systems provided by the present invention is when carrying out the production of mass-producing cultivation Nostoc; only nostoc sphaeroides need be put into surrounding is stainless steel frame; in the culture plate with holes that the stainless (steel) wire that bottom is about aperture 1mm is made, be placed on culturing rack and cultivate.
Many researchists drop into a large amount of manpower and materials the artificial culture method of Nostoc are studied in recent years, at present, the artificial culture method of all relevant Nostoc all comprises the links such as prepared by nutrient solution preparation, algae kind, aerated culture, hardening treatment, screening is gathered in the crops and be dried, and culture vessel is glass jar or raceway pond.These methods all exist production link many, and water loss is large, and energy consumption is high, and spheroid easily breaks, the problem such as the low and production cost of dry matter content is high.Therefore we have designed brand-new Nostoc ecological simulation culture systems and method, can effectively address these problems.
No matter the culture vessel using is glass jar, bio-reactor, pond or raceway pond, all need Nostoc to mix with nutrient solution, in whole culturing process, must Nostoc be suspended in nutrient solution by ventilation or impeller, in the culture cycle of nearly 90 days, every 7～10 days, need to change a nutrient solution, before results, in order to improve the physical strength of spheroid, also need to carry out hardening treatment.In new culture systems, we simplify step, removed ventilation, have cleaned, change the processes such as nutrient solution, sclerosis, can save a large amount of manpowers, effectively increase work efficiency, and reduce production costs.
In new culture systems, we rest on Nostoc in culture plate and do not need Nostoc to carry out suspension culture, thereby can economize on water more than 80%; Temperature control neither be undertaken by controlling whole culture space, and only need carry out temperature control to nutrient solution, just can reach the temperature condition that makes culture remain on suitable growth, thereby can energy efficient more than 50%.
In new culture systems, can effectively suppress assorted algae pollution, compare with traditional method to reduce and pollute more than 70%.
In culturing process, to break be an insoluble problem to Nostoc spheroid always.This is mainly owing to needing to pass into large quantity of air in culturing process, Nostoc to be suspended in nutrient solution, and the violent surging force of air-flow and make Nostoc spheroid subject to damage with the friction of culture vessel, broken serious, general percentage of damage is between 5%-10%, broken serious in more than 10%, when changing nutrient solution, even can all break.And in the process that use present method is cultivated, Nostoc spheroid is damaged few, breakage rate approaches zero.
In traditional cultural method, because Nostoc grows in the aqueous solution, its water content is very high, and dry matter content generally only has 1.5-2% left and right.And its dry matter content of the Nostoc of cultivating by method can, up to 8%, be 2-5 times of traditional method.Because dry matter content is high, thereby physical strength is high, therefore in culturing process, does not need to carry out special process of setting.
Compared with prior art, advantage of the present invention is:
1, to produce the required technology and equipment of Nostoc simple for Nostoc ecological simulation production system, invests littlely, and production cost is low and can annually produce continuously;
While 2, using present method, the light of Nostoc, warm subject range are wider, and temperature is at 4-35 ℃, and illumination all can be grown at 600-12000lx, and photosynthetic efficiency is high;
3, Nostoc dry matter content is than being suspended in cultivate in nutrient solution high 2～3 times, and dry matter content can reach 7～8%, is 2～5 times of other cultural method;
4, Nostoc is mixed, and algae pollution rate is low, good stress resistance, and spheroid does not break;
5, owing to only culture-liquid temp being controlled, can effectively reduce energy consumption, save energy reaches more than 50%;
6, in culturing process, can economize on water more than 80%;
7, nutrient solution composition and potential of hydrogen, close to natural water, can be recycled, and directly discharge does not pollute the environment yet.
Accompanying drawing explanation
Fig. 1 culture systems structural representation of the present invention;
Fig. 2 culture plate structure for amplifying schematic diagram with holes of the present invention;
Wherein, the 1st, Multilayer culture shelf, the 2nd, culture plate with holes, the 3rd, illumination apparatus, the 4th, nutrient solution recovering tray, the 5th, recovery channel, the 6th, reclaim liquid filtering system, the 7th, nutrient solution pond for recovering, the 8th, nutrient solution transferpump, the 9th, temperature control device, the 10th, nutrient solution delivery controller, the 11st, nutrient solution transport pipe, the 12nd, shower nozzle, the 13rd, vertical support bar, the 14th, horizontal support bar.
Embodiment
Below in conjunction with embodiment, the present invention is described further.
Embodiment 1
Nostoc Algal Ecology simulation culture systems, comprise nutrient solution pond for recovering 7, also comprise by a plurality of passive vertical support bars 13 and a plurality of Multilayer culture shelf 1, a plurality of illumination apparatus 3, recovery liquid filtering system 6, nutrient solution delivery controller 10, nutrient solution transport pipe 11, at least one shower nozzle 12 forming for placing the horizontal support bar 14 of culture plate 2 with holes; Described illumination apparatus 3 is arranged on the below of any one deck horizontal support bar 14 of Multilayer culture shelf 1, described Multilayer culture shelf 1 bottom is provided with nutrient solution recovering tray 4, and described nutrient solution recovering tray 4 is communicated with nutrient solution pond for recovering 7 by recovery channel 5, recovery liquid filtering system 6; In being communicated with, described nutrient solution pond for recovering 7 is provided with nutrient solution transferpump 8 and temperature control device 9; Described nutrient solution transferpump 8 is communicated with shower nozzle 12 by nutrient solution transport pipe 11, and described shower nozzle 12 is positioned at the top of Multilayer culture shelf 1 top layer.
Described nutrient solution transport pipe 11 is provided with nutrient solution delivery controller 10.Described culture plate with holes 2 is netted body of stainless steel, aperture≤1mm.The interlamellar spacing of described Multilayer culture shelf 1 is 30cm-40cm.Described shower nozzle 12 is 40cm-50cm with the distance of Multilayer culture shelf 1 top layer.
Embodiment 2
Take Nostoc as example, and Nostoc ecological simulation cultural method, cultivates by culture systems described in embodiment 1, specifically comprises:
Cultivate place and equipment: take glasshouse or plastic greenhouse greenhouse as cultivating place, described culture systems is placed in and is cultivated in place, culture plate with holes is placed on Multilayer culture shelf, the every interlayer of Multilayer culture shelf is divided into 30cm～40cm, culture plate aperture≤1mm with holes, the distance that shower nozzle and Multilayer culture shelf top layer are set is 40cm-50cm;
Light source: the Nostoc required light intensity of growing is 1000-8000lx, can, without source of artificial light, when light intensity is too high, need reduce light intensity by sunshade net when natural light light intensity meets this condition.Ordinary Light Sources Have is water-proof daylight lamp pipe or LED lamp, and these lamps are arranged under the horizontal frame of culturing rack, can obtain the irradiation of 1000-8000lx light intensity to maintain frond.
Temperature: the growth temperature range of Nostoc is 10-35 ℃, optimum temps is 20-25 ℃ of left and right.When natural temperature is in this temperature range, only need open greenhouse side form and teleblem carries out ventilation; When temperature is too low or too high, need be by regulating culture-liquid temp and spray to make culture keep suitable temperature interval time.
Nutrient solution composition and concentration are:
Prepared by algae kind: nostoc sphaeroides divides two kinds of firsts and seconds.When wherein prepared by First stage algae seeds, first with distilled water, prepare nutrient solution, then divide and install to 50-l000ml Erlenmeyer flask or reagent bottle, sealing was by 121 ℃ of sterilizings of high pressure 20 minutes, cooling standby; Then select vigorous, the shapeliness of growth, good springiness and free of contamination Nostoc spheroid, in laboratory, under strict aseptic condition, carry out disinfection, be inoculated into the substratum preparing after homogenate, pass into filtrated air and carry out aerated culture; During cultivation, temperature is controlled at 20-25 ℃, and illumination is controlled at 3000-5000lx, and adds appropriate ruddiness.When frond is cultivated diameter 0.5-1mm, be transferred in 10-20L carboy, carry out enlarged culturing, culture condition is the same; When frond diameter grows to 2mm left and right, check good, the pollution-free secondary algae kind production kind that can be used as of growth conditions).
Inoculation and cultivation: the fine quality production kind of preparing by above method is put into the culture plate with holes of bottom part aperture diameter 1mm, the density of inoculation is controlled at 0.5g/cm
2～1g/cm
2inoculation thickness is 1-2cm, then culture plate layering with holes is placed on culturing rack, and culture-liquid temp is controlled to 20-25 ℃, by certain time interval, in dish, spray nutrient solution, be as the criterion interval time to keep culture surface wettability and to make culture temperature remain on 15-30 ℃; In illumination, cross and should or supplement illumination by source of artificial light by sunshade when strong or not enough and make it meet culture condition.The nutrient solution of spray is successively leaked down to lower floor by upper strata, finally fall into undermost nutrient solution catch tray, by collector tray bottom, export and flow into collection tube again, equipment enters nutrient solution collecting tank after filtering after filtration, by temperature controlling instruments, culture-liquid temp is adjusted to 20-25 ℃ of winter temperature more slightly high, summer temp is slightly low), then re-use by spray piping.In collecting tank, the concentration of nutrient solution composition must be monitored, and loss part must be supplied in time.In culturing process, need stir Nostoc 1-2 time every day, when Nostoc increment is double, assign in two culture plates with holes and to continue to cultivate, by that analogy, until Nostoc grows into 6-8mm, cross after screen sizing direct marketing or dry in the shade after sell.
Product detects: by being observed visually the Nostoc spheroid situation of not breaking, effectively suppressed assorted algae pollution, compared to reduce pollute more than 70% with traditional method, by drying rear detection, shown that Nostoc dry matter content is 7%-8%.
Claims (7)
1. Nostoc Algal Ecology is simulated culture systems, comprise nutrient solution pond for recovering (7), it is characterized in that, also comprise by a plurality of passive vertical support bars (13) and a plurality of Multilayer culture shelf (1), a plurality of illumination apparatus (3), recovery liquid filtering system (6), nutrient solution delivery controller (10), nutrient solution transport pipe (11), at least one shower nozzle (12) forming for placing the horizontal support bar (14) of culture plate with holes (2); Described illumination apparatus (3) is arranged on the below of any one horizontal support bar (14) of Multilayer culture shelf (1), described Multilayer culture shelf (1) bottom is provided with nutrient solution recovering tray (4), and described nutrient solution recovering tray (4) is communicated with nutrient solution pond for recovering (7) by recovery channel (5), recovery liquid filtering system (6); Described nutrient solution pond for recovering (7) is provided with nutrient solution transferpump (8) and temperature control device (9) in being communicated with; Described nutrient solution transferpump (8) is communicated with shower nozzle (12) by nutrient solution transport pipe (11), and described shower nozzle (12) is positioned at the top of Multilayer culture shelf (1) top layer.
2. Nostoc Algal Ecology is simulated culture systems according to claim 1, it is characterized in that, described nutrient solution transport pipe (11) is provided with nutrient solution delivery controller (10).
3. Nostoc Algal Ecology is simulated culture systems according to claim 1, it is characterized in that the aperture≤1mm of described culture plate with holes (2).
4. Nostoc Algal Ecology is simulated culture systems according to claim 1, it is characterized in that, the interlamellar spacing of described Multilayer culture shelf (1) is 30 cm-40cm.
5. Nostoc Algal Ecology is simulated culture systems according to claim 1, it is characterized in that, described shower nozzle (12) is 40cm-50cm with the distance of Multilayer culture shelf (1) top layer.
6. Nostoc Algal Ecology simulation cultural method, is characterized in that, right to use requires one of 1-5 described culture systems, and concrete steps are:
(1) take glasshouse or plastic greenhouse greenhouse as cultivating place, the described culture systems of one of claim 1-5 is placed in and is cultivated in place, culture plate with holes is placed on Multilayer culture shelf, the every interlayer of Multilayer culture shelf is divided into 30 cm～40cm, culture plate aperture≤1mm with holes, the distance that shower nozzle and Multilayer culture shelf top layer are set is 40cm-50cm;
(2) inoculation, by algae kind for the algae kind that the diameter that obtains by sterile culture is 1mm～2mm, is evenly laid in algae kind in culture plate with holes during inoculation, and the density of inoculation is controlled at 0.5 g/cm
2～1g/cm
2, inoculation thickness is 1 cm-2cm;
(3) on culture, spray nutrient solution, spray 10～30 minutes interval times, each 1～2 minute time length of spray, the adjusting of time take that to keep all culture surface wettabilities be standard, nutrient solution is dripped to lower floor by upper strata, finally flow to the nutrient solution catch tray of the lowest layer, then be recovered to nutrient solution pond for recovering, recycle; Meanwhile, controlled light remains between 1000lx～20000lx, controls culture-liquid temp and remains between 20 ℃～30 ℃;
Described nutrient solution composition and concentration are:
MgSO
4. 7H
2O 0.05-0.15g/L
K
2HPO
4. 3H
2O 0.005-0.08g/L
CaCl
2. 2H
2O 0.004-0.06g/L
EDTA-Na
2 0.001-0.004g/ L
Ferric ammonium citrate 0.001-0.005 g/ L
H
3BO
4 0.002-0.008mg/L
Na
2MoO
4.2H
2O 0.001-0.030mg/L
ZnSO
4.7H
2O 0.001-0.02mg/L
CuSO
4.5H
2O 0.001-0.005mg/L
MnCl
2.4H
2O 0.020-0.060mg/L。
7. Nostoc Algal Ecology is simulated cultural method according to claim 6, it is characterized in that, described Nostoc algae is Nostoc or Nostoc commune.
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| CN104178426A (en) * | 2014-08-08 | 2014-12-03 | 新奥科技发展有限公司 | Method and device for controlling culture temperature of microalgae |
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