CN1089427A - A kind of method for quickly growing seedlings of laver - Google Patents

A kind of method for quickly growing seedlings of laver Download PDF

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Publication number
CN1089427A
CN1089427A CN 93112319 CN93112319A CN1089427A CN 1089427 A CN1089427 A CN 1089427A CN 93112319 CN93112319 CN 93112319 CN 93112319 A CN93112319 A CN 93112319A CN 1089427 A CN1089427 A CN 1089427A
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China
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filamentous
culture
days
hour
light intensity
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CN 93112319
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Chinese (zh)
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王素娟
沈怀舜
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Shanghai Ocean University
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Shanghai Ocean University
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Priority to CN 93112319 priority Critical patent/CN1089427A/en
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Pending legal-status Critical Current

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Abstract

The present invention relates to algae culturing, especially the porphyra yezoensis free conchocelis is cultivated and collection technique.The technical problem that the present invention solves is the method that the porphyra yezoensis free conchocelis is grown seedlings.It is characterized in that adopting the filamentous chopping of porphyra yezoensis kind frustule cultivation to put into MES culture fluid inflation suspension culture about 50 days, fix the breed of plunging into the commercial sea in second day when filamentous conchospore branch appearance two minutes or near two timesharing with phycocolloid with organizing cracker to pulverize to be sprinkling upon on the nettle.This method can be used for extensive laver culture production.

Description

The present invention relates to algae culturing, especially the cultivation of porphyra yezoensis free conchocelis and collection technique.
Reported that Porphyra haitanensis reaches the technology of directly collecting seedling by cultivating free conchocelis in March, 1980 " aquatic product journal ", this technology is to dry planting vegetables 1-2 hour, scrub to kill through the sterilization seawater again and be attached to the assorted algae that plants vegetables, to be placed on through the carpospore that planting vegetables of abatement processes gathered in the seawater of abundant nutritive salt and cultivate, make filamentous in seawater suspension growth or on chamber wall apposition growth, after cultivating five months, filamentous is carried out stimulation by running water and reduce water temperature gradually and impel it to diffuse spore directly to collect seedling at night, this method is taked few transplanting for preventing assorted algae pollution, do not shake, stuffiness, the measure of not stirring, the filamentous poor growth, cultivate and only increased weight about 10 times in five months, and exist maturity low, diffuse the problem of conchospore difficulty, can't adapt to the requirement that large-scale farming production is collected seedling on time and plunged into the commercial sea and culture, for also on producing, the do not collect seedling precedent of success of porphyra yezoensis free conchocelis.
The purpose of this invention is to provide a kind of porphyra yezoensis free conchocelis fast culture and collection technique, the porphyra yezoensis free conchocelis is grown seedlings can be used in large-scale farming is produced.
The present invention adopts the upper semisection of ripe porphyra yezoensis kind algae to wash the filamentous that the chopping enzymolysis obtains cell culture with the sterilization seawater, filamentous is put into MES culture fluid filter-sterilized charge of air suspension culture with organizing cracker to be chopped into 400-500 μ length, the early stage of cultivating, mid-term, later stage is with water temperature, illumination, condition of culture such as nutrition are regulated by following requirement: early stage was for 20-30 days, 20 ℃ of temperature, light intensity 3000-4000Lux, light time 18-24 hour, the MES culture fluid is mended microcosmic salt to 3PPM, about 20 days mid-terms, temperature 23-26 ℃, light intensity 1000-2000Lux, light time 10-12 hour, the MES culture fluid is mended microcosmic salt to 5PPM, about 10 days later stages, temperature 18-20 ℃, light intensity 500-800Lux, light time 6-10 hour, nitrogen salt is reduced to 0PPM gradually from 10PPM, microcosmic salt increases to 10PPM, when filamentous conchospore branch occurred two fens or near two timesharing with organizing cracker to shred 1 minute, evenly be sprinkling upon on the nettle according to requirement, dry slightly with fixing the immersion immediately of phycocolloid and contain in the 0.1M calcium chloride seawater 5 minutes and put into fresh seawater and spend the night, the breed of plunging into the commercial sea in second day.
Because the present invention adopts the inflation suspension culture and takes to control condition of culture such as temperature, illumination, nutrition, filamentous increases fast, through cultivating weightening finish in 50 days 13.4 trainings, and expand algal filament and reach ripe, by the chopping amplification, cultivate 20-30 mu seedling and take up an area of only 0.5 square metre stereoscopic culture frame, simultaneously also owing to can cultivate in culturing room, be not subjected to weather effect, can adapt to the needs that large-scale farming is produced.
Realize that best mode of the present invention is to adopt copper sulphate sterilised filtration air, aeration quantity in the culture fluid is pressed 4-5 and is risen culture fluid per minute inflation 2-5 liter, early stage light intensity 3500Lux, the light time of condition of culture shortened to 12 hours gradually from 24 hours, temperature was reduced to 23 ℃, light intensity gradually from 26 ℃ and was reduced to 1000Lux, light time gradually from 1500Lux and reduced to 10 hours from 12 hours mid-term, and the late stage of culture temperature is reduced to 18 ℃, light intensity gradually from 20 ℃ and reduced to 500Lux, light time gradually from 800Lux and reduced to gradually 6 hours.

Claims (1)

1, a kind of method for quickly growing seedlings of laver, the upper semisection that it is characterized in that adopting ripe porphyra yezoensis kind algae washes the filamentous that shreds the cell culture that enzymolysis obtains with the sterilization seawater, with filamentous with organizing cracker to be chopped into 400-500 μ length, put into MES culture fluid filter-sterilized charge of air suspension culture, regulating temperature in the 20-30 days early stage of cultivating is 20 ℃, light intensity 3000-4000Lux, light time 18-24 hour, culture fluid is mended microcosmic salt to 3PPM, 20 days left and right sides temperature 23-26 ℃ of mid-term, light intensity 1000-2000Lux, light time 10-12 hour, culture fluid is mended microcosmic salt to 5PPM, later stage 10 days left and right sides temperature 18-20 ℃, light intensity 500-800Lux, light time 6-10 hour, nitrogen salt is reduced to OPPM gradually from 10PPM, microcosmic salt increases to 10PPM; When filamentous conchospore branch occurred two fens or near two timesharing with organizing cracker to shred 1 minute, evenly be sprinkling upon on the nettle according to requirement, dry slightly with fixing the immersion immediately of phycocolloid and contain in the 0.1M calcium chloride seawater about 5 minutes and put into fresh seawater and spend the night, the breed of plunging into the commercial sea in second day.
CN 93112319 1993-01-15 1993-01-15 A kind of method for quickly growing seedlings of laver Pending CN1089427A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 93112319 CN1089427A (en) 1993-01-15 1993-01-15 A kind of method for quickly growing seedlings of laver

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Application Number Priority Date Filing Date Title
CN 93112319 CN1089427A (en) 1993-01-15 1993-01-15 A kind of method for quickly growing seedlings of laver

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CN1089427A true CN1089427A (en) 1994-07-20

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CN 93112319 Pending CN1089427A (en) 1993-01-15 1993-01-15 A kind of method for quickly growing seedlings of laver

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Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1328950C (en) * 2004-04-26 2007-08-01 中国科学院海洋研究所 Seedling raising method for non-shell protonema cell of laver
CN100337532C (en) * 2004-04-26 2007-09-19 中国科学院海洋研究所 Seedlings raising method for non-shell monospore cell of laver
CN101167439B (en) * 2007-11-16 2010-04-21 中国海洋大学 Polysiphonia urceolata tetraspore seedling formation method
CN101946683A (en) * 2010-07-29 2011-01-19 大连海洋大学 Method for collecting seedlings from scytosiphon filaments
CN103651095A (en) * 2013-12-26 2014-03-26 安徽理工大学 Large-scale seaweed seedling culturing and transplanting method
CN103907527A (en) * 2014-03-31 2014-07-09 江苏省海洋水产研究所 Porphyra yezoensis germplasm propagation expanding method
CN104106462A (en) * 2014-07-04 2014-10-22 常熟理工学院 Method for applying and popularizing porphyra yezoensis fine seeds
CN104663419A (en) * 2015-01-16 2015-06-03 浙江省海洋水产研究所 Gulfweed transplanting method based on juvenile sporophyte adhesion function
CN104782471A (en) * 2015-05-14 2015-07-22 海安县兰波实业有限公司 Large-scale seedling breeding method for porphyra yezoensis
CN105993909A (en) * 2016-05-13 2016-10-12 集美大学 Pyropia haitanensis pure line seedling cultivation method
CN106489711A (en) * 2016-10-09 2017-03-15 盐城海瑞食品有限公司 Porphyra haitanensis nursery pond water purification process
CN106489712A (en) * 2016-10-09 2017-03-15 盐城海瑞食品有限公司 The seedling raising process of porphyra haitanensis
CN106489713A (en) * 2016-10-09 2017-03-15 盐城海瑞食品有限公司 The method for culturing seedlings of porphyra haitanensis
CN106818009A (en) * 2017-04-18 2017-06-13 山东大学 Phosphorus limits the benefit phosphorus method for increasing of potential eutrophy sea area sea-tangle raft culture
CN109906934A (en) * 2019-03-27 2019-06-21 中国科学院海洋研究所 A method of promoting the formation of Porphyra yezoensis filamentous conchosporangia
CN110036902A (en) * 2019-04-24 2019-07-23 大连海事大学 Marine Red Alga Polysiphonia Urceolata filiform clone method for culturing seedlings
CN110073966A (en) * 2019-06-13 2019-08-02 国家海洋环境监测中心 Based on the seaweed spring method for culturing seedlings for expanding training seedling in algal filament room
CN110771495A (en) * 2019-11-27 2020-02-11 山东省海洋生物研究院 Method for reducing aberration rate of parthenogenesis sporophyte of kelp germplasm

Cited By (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100337532C (en) * 2004-04-26 2007-09-19 中国科学院海洋研究所 Seedlings raising method for non-shell monospore cell of laver
CN1328950C (en) * 2004-04-26 2007-08-01 中国科学院海洋研究所 Seedling raising method for non-shell protonema cell of laver
CN101167439B (en) * 2007-11-16 2010-04-21 中国海洋大学 Polysiphonia urceolata tetraspore seedling formation method
CN101946683A (en) * 2010-07-29 2011-01-19 大连海洋大学 Method for collecting seedlings from scytosiphon filaments
CN103651095A (en) * 2013-12-26 2014-03-26 安徽理工大学 Large-scale seaweed seedling culturing and transplanting method
CN103651095B (en) * 2013-12-26 2015-03-11 安徽理工大学 Large-scale seaweed seedling culturing and transplanting method
CN103907527A (en) * 2014-03-31 2014-07-09 江苏省海洋水产研究所 Porphyra yezoensis germplasm propagation expanding method
CN104106462A (en) * 2014-07-04 2014-10-22 常熟理工学院 Method for applying and popularizing porphyra yezoensis fine seeds
CN104106462B (en) * 2014-07-04 2016-09-14 常熟理工学院 A kind of method of Porphyra yezoensis Ueda breeding application
CN104663419A (en) * 2015-01-16 2015-06-03 浙江省海洋水产研究所 Gulfweed transplanting method based on juvenile sporophyte adhesion function
CN104782471A (en) * 2015-05-14 2015-07-22 海安县兰波实业有限公司 Large-scale seedling breeding method for porphyra yezoensis
CN105993909B (en) * 2016-05-13 2019-08-27 集美大学 A kind of porphyra haitanensis pure lines seedling fostering method
CN105993909A (en) * 2016-05-13 2016-10-12 集美大学 Pyropia haitanensis pure line seedling cultivation method
CN106489712A (en) * 2016-10-09 2017-03-15 盐城海瑞食品有限公司 The seedling raising process of porphyra haitanensis
CN106489713A (en) * 2016-10-09 2017-03-15 盐城海瑞食品有限公司 The method for culturing seedlings of porphyra haitanensis
CN106489711A (en) * 2016-10-09 2017-03-15 盐城海瑞食品有限公司 Porphyra haitanensis nursery pond water purification process
CN106818009A (en) * 2017-04-18 2017-06-13 山东大学 Phosphorus limits the benefit phosphorus method for increasing of potential eutrophy sea area sea-tangle raft culture
CN106818009B (en) * 2017-04-18 2019-07-30 山东大学 Phosphorus limits the benefit phosphorus method for increasing of potential eutrophy sea area kelp raft culture
CN109906934A (en) * 2019-03-27 2019-06-21 中国科学院海洋研究所 A method of promoting the formation of Porphyra yezoensis filamentous conchosporangia
CN110036902A (en) * 2019-04-24 2019-07-23 大连海事大学 Marine Red Alga Polysiphonia Urceolata filiform clone method for culturing seedlings
CN110073966A (en) * 2019-06-13 2019-08-02 国家海洋环境监测中心 Based on the seaweed spring method for culturing seedlings for expanding training seedling in algal filament room
CN110771495A (en) * 2019-11-27 2020-02-11 山东省海洋生物研究院 Method for reducing aberration rate of parthenogenesis sporophyte of kelp germplasm

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