CN111543305A - Simple and efficient kelp seedling culture method by improving seedling curtain dark treatment technology - Google Patents
Simple and efficient kelp seedling culture method by improving seedling curtain dark treatment technology Download PDFInfo
- Publication number
- CN111543305A CN111543305A CN202010545612.4A CN202010545612A CN111543305A CN 111543305 A CN111543305 A CN 111543305A CN 202010545612 A CN202010545612 A CN 202010545612A CN 111543305 A CN111543305 A CN 111543305A
- Authority
- CN
- China
- Prior art keywords
- seedling
- kelp
- dark
- curtain
- production
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Cultivation Of Seaweed (AREA)
Abstract
The invention discloses a simple, convenient and efficient kelp seedling raising method for improving a seedling curtain dark processing technology, wherein a seedling raising attachment base is replaced by a northern traditional red brown rope into a vinylon rope, and the low density level of embryo spores in 10-15/160 times of the visual field of a microscope is controlled in a spore collection stage; after spore collection is finished, the seedling curtains are directly stacked in multiple layers in a dark environment, stored in running water at a low temperature and then normally cultured in a single-layer tiled seedling raising pool. The invention has the advantages that: the seedling culture method is suitable for large-scale kelp seedling production, high-quality seedlings can be obtained, the seedling curtain dark treatment technical operation is simplified, the capacity and production investment of a production workshop occupied by the early stage seedling culture production stage of kelp are reduced, the normal seedling culture time is shortened, and the production cost is greatly reduced.
Description
Technical Field
The invention relates to a seedling raising method for kelp. Belongs to the technical field of mariculture.
Background
China is a large country for kelp cultivation, and the cultivation sea area has Liaoning in the north, and is from south to Fujian. As the seedling production method used in the prior art, zoospores scattered by the planted kelp are collected onto a proper attaching base by adopting an artificial method, and the seedlings reach the standard specified in GB/T15807-2008 through technical measures such as temperature control, light control, fertilization and the like and a cultivation process in a seedling raising room.
Aiming at the defects of long seedling growing period, high cost and the like of the traditional kelp, a scholars proposes that a seedling curtain (gametophyte stage) is stored under a low-temperature dark condition to shorten the multi-culture time that the seedlings cannot be delivered from the warehouse and the sea due to high natural seawater temperature, such as the research of an improved kelp seedling culture method in summer, which is published in the marine science, 1984, 3: 38-42, and the research of the seedling shedding rotten seedling method in a seedling growing system (hereinafter, referred to as document 1) and the preventive measure thereof (hereinafter, referred to as document 1) and the research of the improved kelp seedling culture method in summer, which is published in the marine science, 1986, 10(5): 24-27), and the analysis of the seedling shedding and rotten seedling reasons and the preventive measure thereof in a summer kelp growing system (hereinafter, referred to as document 2). For another example, chinese patent application publication No. CN101617623A discloses "a kelp seedling raising curtain and kelp seedling raising technique" (hereinafter referred to as document 3), and chinese patent publication No. CN 102668966B discloses an "energy-saving kelp seedling raising technique" (hereinafter referred to as document 4), and japanese science, houxu light, li xinghua are disclosed in "marine science, 2015, 39(3):106 and 110, the dark running water storage gametophyte (hereinafter referred to as literature 5), replacing a red palm rope attachment group with a vinylon and polyethylene blended attachment group, collecting spores, culturing for 5-16 days, when the gametophyte is mature, the seedling curtain is put into a dark refrigeration house at the temperature of 1-5 ℃ or a dark flowing water pool at the higher temperature of 10-15 ℃ for long-time storage, then the seedling curtain is moved to a higher temperature or even to a high temperature of 15-20 ℃ for normal seedling culture according to the date of the seedlings going to the sea.
The seedling raising methods reported in documents 1 to 5 require normal cultivation for a certain time under low-temperature illumination after spore collection, and then perform seedling curtain dark treatment. The seedling raising method has the disadvantages of complicated operation and no benefit for the method of cultivating first and then dark processing. The cultivation before the dark treatment ensures that the kelp zoospores germinate into gametophytes from the embryospores after attaching the kelp zoospores, and the seedlings are switched into the reproductive development and juvenile sporophyte stage from the vegetative growth stage of the gametophytes after cultivating for 5-16 days in documents 3-5 after collecting the spores, so that the seedlings are fragile and light-sensitive, and the dark treatment is not beneficial to the growth of the seedlings. It was also found in document 1 that gametophytes are suitable for low-temperature dark storage, whereas juvenile sporophytes cannot be stored in low-temperature dark. In the document 5, after spore collection, the seedlings are cultivated for 6-10 days at 10 +/-0.5 ℃, and the seedlings only reach the three types of seedling curtain standards specified in GB/T15807-2008 after being stored for 14 days in dark.
The seedling raising methods reported in documents 1 and 3 require a refrigeration house for storage after spore collection, and the investment of newly-built refrigeration equipment facilities is increased.
The seedling raising methods reported in the documents 1 to 5 all need high-density seedling collection (20-30 to 50-60 free spores/160-fold microscope visual field). This compensates for the death of the offspring seeds caused by dark treatment (50% of gametophytes die after 12 days of cultivation after collecting spores and 14 days of storage in still water at low temperature (document 2)), but increases the cost of growing the kelp. Meanwhile, the high-density seedling collection ensures that algae body epidermis, algin and the like which fall off when a large number of kelp seeds diffuse spores are attached to a seedling curtain, and a large number of seedlings die in the dark treatment process, so that the seedling culture environment is severe; the high-density seedling culture can also influence the growth of seedlings, particularly seedlings in the later period of seedling culture, and the seedling culture management cost is increased.
According to NY/T5057-2001 industry standard, the temperature range of the whole kelp seedling raising period is 5.5-10 ℃. The low temperature not only reduces the metabolism of the seedlings, but also inhibits the growth of epiphytic bacteria and other algae, and is beneficial to the preservation of the seedlings (documents 1-2). Dark treatment is carried out at 10-15 ℃ (documents 4-5), although energy consumption is saved, under the temperature condition, the seedling metabolism is vigorous, the growth of epiphytic bacteria and heterophyta is accelerated, the dark treatment seriously affects the gametophyte development and the survival rate of juvenile sporophytes, and the production instability is increased.
In document 4, after the seedling curtain is treated in the dark, the temperature of the conventional cultivation water is increased to 15-20 ℃, which exceeds the temperature range suitable for growth and development of kelp seedlings, and the epiphytic bacteria and the miscellaneous algae are propagated in a large quantity, so that the survival rate of the seedlings is low, the morbidity is high, the production is seriously influenced, and the method is not suitable for large-scale production. Document 5 also demonstrates that the spore harvest begins 12 days of dark treatment (10 ± 0.5 ℃) at day 8, and the seed curtains can only reach the second class standard even if the normal cultivation temperature is 10-11 ℃; the seed curtains do not reach the three standards after being treated in the dark for 16 days.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a simple and efficient kelp seedling raising method which is suitable for large-scale production and has simple operation and low production cost. Through the adjustment of the adhesion base and the change of the cultivation mode in the early stage of seedling cultivation, the normal seedling cultivation time is shortened, the capacity of a production workshop occupied by the early stage seedling cultivation production stage is reduced, the production investment in the early stage of seedling cultivation is reduced, and the purpose of reducing the total production cost is achieved.
The technical scheme of the invention is as follows:
a simple and efficient kelp seedling culture method for improving a seedling curtain dark processing technology is characterized by comprising the following steps of:
1) in the spore collection stage, the low density level of the embryo spores in the 10-15/160 times microscope visual field is controlled when the seedling culture attachment base is used for attachment; the seedling culture attaching base is a light-color vinylon rope;
2) directly superposing the multiple layers of seedling curtains under dark conditions after spore collection, and storing in running water at 7-8 ℃;
3) and taking out the seedling curtain, and culturing in a single-layer tiled seedling raising pool according to a normal production process flow.
The light color described in step 1) is preferably white.
The dark condition in the step 2) is in a nursery pond covered with a shading material.
The preservation time in step 2) is preferably not more than 20 days.
The normal production process flow in the step 3) is that the seedling curtains are spread in a seedling raising pool, and the temperature of the culture water is 7-8 ℃; washing once every 3 days, and gradually increasing the washing pressure from 0.1MPa to 0.3 MPa; the illumination intensity 2800 and 10000Lux are adjusted according to the growth condition.
The invention has the positive effects that:
the improvement of the invention is that the seedling curtain is directly dark-treated at the embryo spore stage after the zoospore is attached, the cultivation process before dark treatment is omitted, the seedling curtain out of the warehouse reaches a first class standard, and the quality is improved by more than two grades. The following technical effects are brought in particular:
(1) the method does not need a normal cultivation process before dark treatment of the seedling curtain, simplifies the operation and saves the early-stage production investment. (2) In the kelp seedling raising production process, the attachment base material is replaced by the white vinylon rope from the traditional red brown rope, so that the attachment base treatment process is simplified, the attachment base cost is reduced, the characteristic of good reflection effect of the vinylon rope is fully utilized by the technical means, and the technical means, such as the means for controlling the low density of the blastospores on the attachment base, the storage temperature, the water supply mode and the like, cooperate to construct the storage environment required by the invention, and the survival rate of the seedlings is ensured. (3) And the seedling is picked at low density, namely more seedling curtains can be picked by the same amount of the seed kelp, so that the cost of seed kelp is saved. (4) The collected seedling curtains are stored in a dark mode in a multi-layer overlapping mode, the capacity of a production workshop and the energy consumption during the dark processing period are greatly reduced, manual operations such as seedling curtain washing and cleaning and the like are not needed, and the production investment in the early stage of seedling culture is reduced. (5) The normal seedling raising time is shortened, and the management cost of the vinylon seedling curtain can be saved by 1/4 compared with the management cost of the northern traditional red palm rope seedling curtain.
The improved seedling curtain dark treatment technology is suitable for large-scale kelp seedling production, seedling raising operation in the early production stage is simplified on the premise of obtaining high-quality seedlings, normal seedling raising time (about 70 days) is shortened by about 1/4, and the quality of the seedling curtain reaches a first-class standard. Reduces the energy consumption, labor and other production costs in the early stage of seedling raising and improves the economic benefit.
Drawings
FIG. 1 is a graph showing the effect of seedling growth obtained in comparative experiments according to examples of the present invention.
Detailed Description
The invention is further illustrated by the following specific examples.
In the production process of kelp seedling, in the spore collection stage, the traditional red brown rope attachment base is replaced by a vinylon rope attachment base, and the density of the embryo spores in the attachment process is controlled to be 10-15/160 times of the microscope visual field. Then directly superposing the seedling curtains for 6 layers, and storing in darkness, running water and at low temperature (7-8 ℃). The holding pool is 1/6 of the normal production pool only. After a certain time of dark treatment, the seedling curtain is taken out and is paved in a seedling pool in a single layer for cultivation according to the normal production process flow. The temperature of the cultivation water is 7-8 ℃; washing once every 3 days, and gradually increasing the washing pressure from 0.1MPa to 0.3 MPa; the illumination intensity 2800 and 10000Lux are adjusted according to the growth condition.
In 2019, the applicant conducted the following comparative experiments.
Collecting seedlings in a seedling raising workshop of a Shandong tobacco station kelp fine variety farm in 2019, 8 and 2 days, controlling the density of embryo spores to be 10-15/160 times of the visual field of a microscope, collecting 400 vinylon seedling curtains, stacking and placing 14 seedling curtains 6 in a seedling raising pond covered with a shading material in a dark and running water preservation mode, and sequentially taking out 2 seedling curtains for normal cultivation management on 12 days, 15 days, 18 days, 21 days, 24 days, 27 days and 30 days after seedling collection. The experiment uses the normally cultivated red brown rope seedling curtain and the vinylon seedling curtain as the contrast. As shown in figure 1, the density and the quality of the seedling curtain after dark treatment for 11, 14 and 17 days are basically the same as those of the control group after the seedling raising period of 10 months and 10 days, the average length of juvenile sporophytes is about 2cm, the juvenile sporophytes are healthy and reach the class of seedling curtain standard specified in GB/T15807-2008. The density of the seedling curtain is obviously reduced after the dark treatment for 20 days, but the production requirement can be basically met; and the seedling density can not meet the production requirement in darkness for more than 23 days. According to the experiment production cost calculation, the kelp seedling culture is carried out according to the technology of the invention, and the production cost is actually saved by about 30%.
FIG. 1 is a diagram of a region A representing a dark processing environment according to an embodiment of the present invention;
FIG. 1, panel B is a comparative image of the seedling curtain and the control curtain after 11-17 days of dark treatment in the example of the present invention; dark treatments 17d, 14d and 11d and a contrast (normally cultured vinylon seedling curtain) are sequentially carried out from left to right;
FIG. 1, area C is a comparative image of the seedling curtain after dark treatment for 11-26 days according to the embodiment of the present invention; dark treatments 26d, 23d, 20d, 17d, 14d and 11d are sequentially performed from left to right;
FIG. 1 is a diagram showing a comparison of sizes of seedlings leaving warehouse in accordance with an embodiment of the present invention; dark treatment for 20d, dark treatment for 17d, dark treatment for 14d and dark treatment for 11d, conventional red brown rope curtain production seedlings (normal cultivation for 70 days) and contrast (vinylon seedling curtain of normal cultivation for 70 days) are sequentially carried out from top to bottom.
As can be seen from FIG. 1, the density and quality of the seedling curtain were almost the same as those of the control group after dark treatment for 17 days or less; the density of the seedling curtain is obviously reduced after dark treatment for 20 days, but the seedling curtain can basically meet the production requirement, but the seedling curtain cannot meet the production requirement after dark treatment for more than 23 days (fig. 1B-C). The size of the seedlings out of the warehouse is basically reduced along with the increase of dark treatment time (no more than 20 days), wherein the difference of the sizes of the seedlings after dark treatment for 11-17 days with the conventional red brown rope seedling curtain is not large and is lower than that of the normally cultivated vinylon seedling curtain. The experimental result shows that the gametophyte development time can be effectively delayed by directly carrying out dark treatment on the seedling curtain after spore collection, the seedling production requirement can be met under the condition that the dark treatment does not exceed 20 days, the normal seedling culture time is shortened, and the seedling culture cost is saved.
Claims (5)
1. A simple and efficient kelp seedling culture method for improving a seedling curtain dark processing technology is characterized by comprising the following steps of:
1) in the spore collection stage, the low density level of the embryo spores in the 10-15/160 times microscope visual field is controlled when the seedling culture attachment base is used for attachment; the seedling culture attaching base is a light-color vinylon rope;
2) directly superposing the multiple layers of seedling curtains under dark conditions after spore collection, and storing in running water at 7-8 ℃;
3) and taking out the seedling curtain, and culturing in a single-layer tiled seedling raising pool according to a normal production process flow.
2. A method for raising seedlings of kelp according to claim 1, characterized in that: the light color in the step 1) is white.
3. A method for raising seedlings of kelp according to claim 1, characterized in that: the dark condition in the step 2) is in a nursery pond covered with a shading material.
4. A method for raising seedlings of kelp according to claim 1, characterized in that: the preservation time in the step 2) is not more than 20 days.
5. A method for raising seedlings of kelp according to claim 1, characterized in that: the normal production process flow in the step 3) is that the seedling curtains are spread in a seedling raising pool, and the temperature of the culture water is 7-8 ℃; washing once every 3 days, and gradually increasing the washing pressure from 0.1MPa to 0.3 MPa; the illumination intensity 2800 and 10000Lux are adjusted according to the growth condition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010545612.4A CN111543305A (en) | 2020-06-16 | 2020-06-16 | Simple and efficient kelp seedling culture method by improving seedling curtain dark treatment technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010545612.4A CN111543305A (en) | 2020-06-16 | 2020-06-16 | Simple and efficient kelp seedling culture method by improving seedling curtain dark treatment technology |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111543305A true CN111543305A (en) | 2020-08-18 |
Family
ID=71997242
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010545612.4A Pending CN111543305A (en) | 2020-06-16 | 2020-06-16 | Simple and efficient kelp seedling culture method by improving seedling curtain dark treatment technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111543305A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113197086A (en) * | 2021-04-15 | 2021-08-03 | 中国科学院海洋研究所 | Gracilaria lemaneiformis cultivation method capable of avoiding cultivation diseases and preferably selecting low-temperature-resistant seedlings |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101617623A (en) * | 2009-08-06 | 2010-01-06 | 梅俊学 | Kelp seedling cultivation curtain and kelp seedling cultivation technology |
| CN102668966A (en) * | 2011-03-13 | 2012-09-19 | 梅俊学 | Energy-saving seed-rearing technology of kelp |
| CN104920201A (en) * | 2015-06-25 | 2015-09-23 | 山东东方海洋科技股份有限公司 | Laminaria germplasm storing method and laminaria germplasm seedling cultivating method |
-
2020
- 2020-06-16 CN CN202010545612.4A patent/CN111543305A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101617623A (en) * | 2009-08-06 | 2010-01-06 | 梅俊学 | Kelp seedling cultivation curtain and kelp seedling cultivation technology |
| CN102668966A (en) * | 2011-03-13 | 2012-09-19 | 梅俊学 | Energy-saving seed-rearing technology of kelp |
| CN104920201A (en) * | 2015-06-25 | 2015-09-23 | 山东东方海洋科技股份有限公司 | Laminaria germplasm storing method and laminaria germplasm seedling cultivating method |
Non-Patent Citations (3)
| Title |
|---|
| 吴瑞敏等: "海带渡夏苗烂苗脱苗预防技术研究", 《福建水产》 * |
| 梅俊学等: "节能型海带育苗新技术――黑暗流水储存配子体", 《海洋科学》 * |
| 王飞久等: "海带苗种培育及无公害养殖技术(二)", 《科学养鱼》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113197086A (en) * | 2021-04-15 | 2021-08-03 | 中国科学院海洋研究所 | Gracilaria lemaneiformis cultivation method capable of avoiding cultivation diseases and preferably selecting low-temperature-resistant seedlings |
| CN113197086B (en) * | 2021-04-15 | 2022-09-23 | 中国科学院海洋研究所 | Gracilaria lemaneiformis cultivation method capable of avoiding cultivation diseases and preferably selecting low-temperature-resistant seedlings |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104365468B (en) | Porphyra yezoensis Ueda shell conchocelis stereoscopic culture system and cultural method thereof | |
| CN112385541B (en) | Method for increasing incidence rate of microspore embryos of black-bone vegetables | |
| CN105993907A (en) | Seedling collecting method and early breeding method of sargassum thunbergii | |
| CN104273037B (en) | A kind of tissue culture and rapid propagation method of tail alpine ash DH33-27 kind | |
| CN100387707C (en) | Clone seedling breeding method for sea-tangle gametocyte | |
| CN111543305A (en) | Simple and efficient kelp seedling culture method by improving seedling curtain dark treatment technology | |
| CN111066648B (en) | Method for promoting formation of sporocyst branches of shells protonema of half-leaf laver | |
| CN104285815B (en) | A kind of tissue culture and rapid propagation method of tail alpine ash DH32-13 kind | |
| CN107232051B (en) | Nutrient rapid propagation-based Sargassum horneri large-scale cultivation method | |
| CN105660466A (en) | Method for breeding grouper fry by controlling illumination | |
| CN109717065B (en) | Method for promoting germination of alfalfa | |
| CN114793902B (en) | Citrus hybrid seed grafted seedling cultivation method | |
| CN102668966B (en) | Energy-saving seed-rearing technology of kelp | |
| CN104221864A (en) | Cryptomeria fortunei clone in-vitro rooting culture method | |
| CN110268971B (en) | Culture method for promoting porphyra haitanensis to appear on market early | |
| CN1663351A (en) | Flower-forcing method for fortunes paulownla seedling | |
| CN107548991B (en) | A kind of inoculation method of lily test tube bulbs | |
| CN114711175B (en) | Method for cultivating black pearl by autologous transplantation of pinctada martensii | |
| CN104304027B (en) | A kind of tissue culture and rapid propagation method of tail alpine ash DH32-29 kind | |
| CN113179853B (en) | White ginseng strain, cultivation site and white ginseng | |
| CN108521929A (en) | Application of the ascorbic acid as termination of diapause accelerating agent in breaking seed dormancy | |
| GB2204591A (en) | Method of preparing an algae culture and a culture medium | |
| CN103340090A (en) | Method for culturing agaricus bisporus kernel microbial strain, namely, kernel cultivated specie, by using white soil as filter layer | |
| CN106665326B (en) | Porphyra haitanensis extending cultivation method | |
| CN117243124A (en) | Method for proliferation culture of lonicera caerulea by applying growth regulator |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200818 |
|
| RJ01 | Rejection of invention patent application after publication |