CN1663351A - Flower-forcing method for fortunes paulownla seedling - Google Patents
Flower-forcing method for fortunes paulownla seedling Download PDFInfo
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- CN1663351A CN1663351A CN 200510064582 CN200510064582A CN1663351A CN 1663351 A CN1663351 A CN 1663351A CN 200510064582 CN200510064582 CN 200510064582 CN 200510064582 A CN200510064582 A CN 200510064582A CN 1663351 A CN1663351 A CN 1663351A
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Abstract
The invention discloses a flower-forcing method for fortunes paulownla seedling comprises the steps of, culturing empress tree sprouts through the conventional tissue culture technique, carrying out root-growing culture through empress tree sprouts for over three generations, nontoxic spouts root generating culture for 10-20 days and transferring into flower bowls, culturing in greenhouses, water sprinkling and deinsectizing with no fertilizer applied. The method can realize flower bud differentiation in the same year of the sprout transplanting.
Description
Technical field
The present invention relates to the flower-forcing method of a kind of seedling, relate in particular to a kind of flower-forcing method of detoxification paulownia tissue cultivating seedling.
Background technology
Paulownia originate in China, are that important speed is given birth to commerical tree species, also are the good plants of afforesting and beautifying environment.Under field conditions (factors), paulownia bloom rule for after entering the age of blooming before and after annual July (because of the area, on the spot changes to some extent) paulownia bud with factor difference such as weather begin to break up.Through differentiation initial stage, calyx form the phase/corolla forms phase, stamen and gynoecium and forms the phase four-stage and finally form bud.Finish these stage needs about 1 month, and survive the winter, bloom April approximately spring next year (China's north and south Different Climatic Zones is variant) with bud.Duration of flowering, the calyx cracking of bud, corolla elongation, corolla is bigger, and purple is to white, bilabiate, five shallow splitting, upper lip two slivers, the lower lip trilobe stretches, warp when blooming, tube usually from 5 millimeters places of base portion to front curve, gradually or suddenly expand more than the knee, make corolla like mitriform, belly has two typhloscles, is flattening downwards near eaves portion place's upper lip, purple dot or purple fringe are often arranged, in gauffer bump pad yellow in the corolla.Four pieces on stamen, the last two, stamen is about half of corolla length.Gynoecium and stamen are isometric or be longer than stamen.Ovary two Room.Fruit is capsule ovum circle shape, and the placenta meat wrinkles more.Ovary after the pollination is expanded the formation fruit rapidly, generally July fruit stop the profile growth, color has green browning, enters the stage of enriching through 80-90 days.The October fruit maturation.So under the normal condition, paulownia are finished the cycle of blossoming and bearing fruit to be needed to stride one at least and survives the winter year.
The flowering naturally age of paulownia is different and variant because of kind and land occupation condition.The most kinds of the paulownia planted in the field enter the reproductive age and generally take 3～5 years, and a few species will arrive more than 6 years.Royal paulownia, Formosan paulownia (Cortex seu Radix Paulowniae kawakamii), river paulownia bloom early, are generally after the field planting 2 to 4 years.Paulownia catalpifolia is bloomed later, general 6 to 9 years, under particular case, finds that also royal paulownia, Paulownia elongata, Formosan paulownia (Cortex seu Radix Paulowniae kawakamii) and fortune paulownia just form the phenomenon of bud (deduction is grown seedlings then, should be the 1st year after the field planting, promptly 2 years living plant) one year.Do not see as yet so far the seedling phase form bud then and finish bloom, the report of fertilization and fruit development overall process.This brings many inconvenience and difficulty for the research of paulownia genetic breeding, has not only increased breeding cycle, has also limited the scope of genetic manipulation.
Summary of the invention
Technical problem to be solved by this invention is to overcome the deficiencies in the prior art, and a kind of flower-forcing method that can make the paulownia seedling bloom, also can finish pollination then is provided.
Technical problem to be solved by this invention realizes by following technological approaches:
A kind of flower-forcing method of paulownia seedling may further comprise the steps:
1, a kind of flower-forcing method of paulownia seedling may further comprise the steps:
1) cultivate detoxification paulownia tissue cultivating seedling according to the conventional organization culture technique,
2) will carry out culture of rootage after the detoxification paulownia tissue cultivating seedling propagation,
3) be transferred to diameter after nontoxic seedling rooting is cultivated and be 8～30 centimetres, height and be in 8～30 centimetres the flowerpot, in the greenhouse, cultivate more than 40 days, condition of culture is in the greenhouse: do not impose any fertilizer, the temperature on greenhouse daytime is controlled in 25～30 ℃ of scopes, and summer, daylight strength control in greenhouse was in 3000～6000 lux scopes.
The condition of carrying out culture of rootage in the above-mentioned flower-forcing method, step 2 wherein) is: medium is MS+IBA 0.1～0.3mg/L+NAA 0.1～0.3mg/L; Cultivation temperature is 25～28 ℃; Step 2) carries out culture of rootage after preferably breeding detoxification paulownia tissue cultivating seedling more than 3 generations in; Preferably nontoxic seedling rooting being cultivated the back in the step 3), to be transferred to diameter after 10～20 days be that 8～10 centimetres, height are in 8～10 centimetres the flowerpot, to cultivate in the greenhouse.
The inventive method can not only make transplants tissue cultivating seedling flower bud differentiation then, and can realize blooming then, and bloom an energy normal mature and finish pollinating process and ovary development of institute, finally can obtain ripe viable seed, this can make the paulownia crossbreeding produce qualitative leap, can make the research of paulownia Developmental Biology do sth. in advance 3～5 years, help promoting the development of paulownia Developmental Biology and tree breeding research than conventional method in the past.
In addition, flower-forcing method of the present invention not only can make paulownia bloom in advance, but also can control simultaneously the flower number that paulownia individual plant bud quantity and individual plant are bloomed simultaneously, reduce the number that comes off of ripe bud, prolong the paulownia florescence, so flower-forcing method of the present invention also has wide practical use aspect paulownia ornamental improving.
Embodiment
Further describe the inventive method by the following examples, it should be understood that these embodiment only are used for the purpose of illustration, never limit the scope of the invention.
[embodiment 1]
Obtaining of 1 paulownia tissue cultivating seedling
Test material: paulownia strain F1.
Newly extract paulownia out branch and be cut into tool joint explant about 1 centimetre, at first under running water, wash a few hours, respectively after 70% alcohol disinfecting 60 seconds, 0.1% mercuric chloride were sterilized 6 minutes, use aseptic water washing again 3 times, being inoculated into then on the common MS medium and carrying out cultured in vitro, is that inoculum carries out successive transfer culture with the tissue cultivating seedling tool leaf stem section of turning out again after 1 month.
2 tissue cultivating seedling detoxification treatment
Tissue culturing seedling's stem section is cut into tool leaf stem section about 1 centimetre, is inoculated in the triangular flask that fills the MS medium every bottle of 1～3 stem section, placing temperature then is that intensity of illumination is 1500 luxs, continuous illumination round the clock in 35 ℃ the illumination box, handled 28 days the temperature difference ± 1.5 ℃.After the stem segment with axillary buds of Temperature Treatment grows up to tissue cultivating seedling, get the stem apex within 0.5 centimetre on the young sprout, (on the MS+2.0～3.0mg/L 6-BA+0.2～4mg/LNAA), temperature is 25 ℃ to be transferred to proliferated culture medium, grow after 20 days, carry out detoxification efficiency with round pcr and detect.The tissue cultivating seedling of the no phytoplasma of proof carries out enrichment culture as detoxic seedling after testing, and with the tool stem segment of proliferated culture medium inoculation original seed tissue cultivating seedling, length is at 2～4 centimetres; Under the illumination cultivation condition, cultivated 30 days.
Behind the enrichment culture, nursery stock is divided into two groups, i.e. experimental group and control group.Experimental group is divided three groups again, and promptly I group, II group and III organize, and every group of strain number is respectively 66,84 and 31.Control group 16 strains.Be transplanted to the nursery, land for growing field crops after the seedling of control group grew about one month and carry out conventional fostering and managing in the greenhouse flowerpot;
The seedling of experimental group takes root transplantation of seedlings in the greenhouse flowerpot, growth surpasses more than 40 days in flowerpot, during this time, adopt flower-forcing method flower forcing of the present invention, concrete grammar is as follows: the nontoxic seedling rooting of experimental group was cultivated after 10 days, in the greenhouse, shine about a week under the natural daylight blake bottle, open then and cultivate bottle cap white silk Miao Yizhou, take out the seedling of taking root at last from blake bottle, wash medium off with clear water, immigration contains in the small flower of the nutrition soil of sterilizing then, the flowerpot size is 8 centimetres for diameter, the basin height is 8 centimetres, and it is ventilative to open the partly plastic film then behind the plastic covering film two weeks, opens plastic foil fully after 3 days.Therebetween according to seedling and the suitable spraying and moisturizing of vermiculite humidity.The normal nutrition pot seedling of growing, water every day, and summer, the greenhouse maximum temperature was controlled at 28 ℃, and night, temperature was reduced to 15 ℃, and Winter-Spring day temperature in autumn is controlled at 25 ℃, and night is about 10 ℃.The greenhouse ceiling covered with the sunshade net and reduced intensity of illumination noon in summer.The daylight strength control is in 3000 luxs.
Result of the test sees Table 1.
[embodiment 2]
Obtaining of 1 paulownia tissue cultivating seedling
Test material: paulownia strain F2.
Newly extract paulownia out branch and be cut into tool joint explant about 2 centimetres, at first under running water, wash a few hours, respectively after 70% alcohol disinfecting 60 seconds, 0.1% mercuric chloride were sterilized 10 minutes, use aseptic water washing again 4 times, being inoculated into then on the common MS medium and carrying out cultured in vitro, is that inoculum carries out successive transfer culture with the tissue cultivating seedling tool leaf stem section of turning out again after 1 month.
2 tissue cultivating seedling detoxification treatment
Tissue culturing seedling's stem section is cut into tool leaf stem section about 1 centimetre, is inoculated in the triangular flask that fills the MS medium every bottle of 1～3 stem section, placing temperature then is that intensity of illumination is 2000 luxs, continuous illumination round the clock in 38 ℃ the illumination box, handled 28 days the temperature difference ± 1.5 ℃.After the stem segment with axillary buds of Temperature Treatment grows up to tissue cultivating seedling, get the stem apex within 0.5 centimetre on the young sprout, (on the MS+2.0～3.0mg/L 6-BA+0.2～4mg/LNAA), temperature is 25 ℃ to be transferred to proliferated culture medium, grow after 20 days, carry out detoxification efficiency with round pcr and detect.The tissue cultivating seedling of the no phytoplasma of proof carries out enrichment culture as detoxic seedling after testing, and with the tool stem segment of proliferated culture medium inoculation original seed tissue cultivating seedling, length is at 2～4 centimetres; Under the illumination cultivation condition, cultivated 40 days.
Behind the enrichment culture, nursery stock is divided into two groups, i.e. experimental group and control group.Experimental group 57 strains, control group 41 strains; Wherein, be transplanted to the nursery, land for growing field crops after the seedling of control group grew about one month and carry out conventional fostering and managing in the greenhouse flowerpot;
The seedling of experimental group take root transplantation of seedlings behind the greenhouse flowerpot always in flowerpot growth surpass more than 40 days, during this time, adopt flower-forcing method flower forcing of the present invention, concrete flower-forcing method is stated as follows: the nontoxic seedling rooting of experimental group was cultivated after 20 days, in the greenhouse, shine about a week under the natural daylight blake bottle, open then and cultivate bottle cap white silk Miao Yizhou, from blake bottle, take out the seedling of taking root at last, wash medium off with clear water, immigration contains in the small flower of the nutrition soil of sterilizing then, and the flowerpot size is 30 centimetres for diameter, and the basin height is 30 centimetres, it is ventilative to open the partly plastic film then behind the plastic covering film two weeks, opens plastic foil fully after 5 days.Therebetween can be according to seedling and the suitable spraying and moisturizing of vermiculite humidity.The normal nutrition pot seedling of growing, water every day, summer, the greenhouse maximum temperature was controlled at 30 ℃, night, temperature was reduced to 15 ℃, Winter-Spring day temperature in autumn control is watered, and summer, the greenhouse maximum temperature was controlled at 29 ℃, and night, temperature was reduced to 15 ℃, Winter-Spring day temperature in autumn is controlled at 26 ℃, and night is about 10 ℃.The greenhouse ceiling covered with the sunshade net and reduced intensity of illumination noon in summer.The daylight strength control is in 4000 luxs.
Result of the test sees Table 1.
Table 1 flower-forcing method of the present invention is to the potted plant flower forcing result of the test of part paulownia strain tissue cultural seedlings of free
| Strain | Handle | Total strain number | The bud differentiation | Bloom | Remarks | ||||
| Transplanting time | The strain number | ?％ | The strain number | ?％ | |||||
| F1 | Flower forcing is handled | I | On March 12nd, 2003 | 66 | ?6 | ?9.09 | ?1 | ?1.52 | To 2003 the end of the year result |
| II | The same | 84 | ?22 | ?25.88 | ?11 | ?12.94 | 2004 annual bearings | ||
| II I | On March 12nd, 2004 | 51 | ?7 | ?13.73 | ?3 | ?5.88 | 2004 annual bearings | ||
| CK | Transplant same I of time in the basin, transplanted June 23 then in nursery, Beijing | 16 | ?0 | ?0 | ?0 | ?0 | 2003 consistent with 2004 annual bearings | ||
| F2 | Flower forcing is handled | On March 12nd, 2003 | 57 | ?4 | ?7.02 | ?2 | ?3.5 | 2004 annual bearings, no flower bud differentiation in 2003 | |
| CK | Same F1-CK | 41 | ?0 | ?0 | ?0 | ?0 | 2003 consistent with 2004 annual bearings | ||
| F3 | Flower forcing is handled | On March 12nd, 2003 | 42 | ?2 | ?4.76 | ?2 | ?14.76 | 2004 annual bearings, no flower bud differentiation in 2003 | |
| CK | Same F1-CK | 15 | ?0 | ?0 | ?0 | ?0 | 2003 consistent with 2004 annual bearings | ||
Above-mentioned result of the test shows that flower-forcing method of the present invention all has significant flower forcing effect to different paulownia strain tissue cultural seedlings of free, illustrates that flower-forcing method of the present invention is stable, effective, practical.
[application examples]
The inventive method can be applicable to the following aspects:
One, potted plant paulownia are as ornamencal flower and tree
Utilize the inventive method annual spring paulownia tissue cultivating seedling original seed to be carried out enrichment culture expansion tissue cultivating seedling base at 27 ℃, night is about 10 ℃.The greenhouse ceiling covered with the sunshade net and reduced intensity of illumination noon in summer.The daylight strength control is in 6000 luxs.
Result of the test sees Table 1.
[embodiment 3]
Obtaining of 1 paulownia tissue cultivating seedling
Test material: paulownia strain F3.
Newly extract paulownia out branch and be cut into tool joint explant about 1.5 centimetres, at first under running water, wash a few hours, respectively after 70% alcohol disinfecting 60 seconds, 0.1% mercuric chloride were sterilized 8 minutes, use aseptic water washing again 3～4 times, being inoculated into then on the common MS medium and carrying out cultured in vitro, is that inoculum carries out successive transfer culture with the tissue cultivating seedling tool leaf stem section of turning out again after 1 month.
2 tissue cultivating seedling detoxification treatment
Tissue culturing seedling's stem section is cut into tool leaf stem section about 1 centimetre, is inoculated in the triangular flask that fills the MS medium every bottle of 1～3 stem section, placing temperature then is that intensity of illumination is 1800 luxs, continuous illumination round the clock in 35～38 ℃ the illumination box, handled 28 days the temperature difference ± 1.5 ℃.After the stem segment with axillary buds of Temperature Treatment grows up to tissue cultivating seedling, get the stem apex within the 0.5cm on the young sprout, (on the MS+2.0～3.0mg/L 6-BA+0.2～4mg/LNAA), temperature is 25 ℃ to be transferred to proliferated culture medium, grow after 20 days, carry out detoxification efficiency with round pcr and detect.The tissue cultivating seedling of the no phytoplasma of proof carries out enrichment culture as detoxic seedling after testing, and with the tool stem segment of proliferated culture medium inoculation original seed tissue cultivating seedling, length is at 2～4 centimetres; Under the illumination cultivation condition, cultivated 35 days.
Behind the enrichment culture, nursery stock is divided into two groups, i.e. experimental group and control group.Wherein, experimental group 42 strains, control group 15 strains.Be transplanted to the nursery, land for growing field crops after the seedling of control group grew about one month and carry out conventional fostering and managing in the greenhouse flowerpot;
The seedling of experimental group take root transplantation of seedlings behind the greenhouse flowerpot always in flowerpot growth surpass more than 40 days, during this time, adopt flower-forcing method flower forcing of the present invention, concrete flower-forcing method is stated as follows: the nontoxic seedling rooting of experimental group was cultivated after 15 days, in the greenhouse, shine about a week under the natural daylight blake bottle, open then and cultivate bottle cap white silk Miao Yizhou, from blake bottle, take out the seedling of taking root at last, wash medium off with clear water, move into then and contain in the small flower of the nutrition soil of sterilizing, the flowerpot size be diameter in 8～20 cm range, the basin height is 8～20 centimetres, it is ventilative to open the partly plastic film then behind the plastic covering film two weeks, opens plastic foil fully after 4 days.Therebetween according to seedling and the suitable spraying and moisturizing of vermiculite humidity.The normal nutrition pot seedling of growing, every day number, carry out culture of rootage then, be transplanted at last in the greenhouse flowerpot, begin to occur flower bud differentiation normal management to June, begin to have bud open to July, corolla opens, bud forms phase from June until October (greenhouse, Beijing area), the flower open season be July to November, the part bud even until blade also can open March next year after coming off fully.Institute's corolla of blooming is big, and purple distributes light delicate fragrance to white, and the normal florescence is 10～15 days (being stretched to corolla from corolla begins to wither).
Break up nearly ripe bud at most at existing condition individual plant and reach 9, individual plant is bloomed 4 at most, blooms 2 at most simultaneously.The inventive method can improve paulownia individual plant bud quantity and reduce the number that comes off of ripe bud then, and improves the flower number that individual plant is bloomed simultaneously.Thereby the paulownia of adopting the inventive method to cultivate can be used as potted plant ornamental flower.For example, the plant that forms ripe bud of adopting the inventive method to cultivate is moved on to other environment, also can normally bloom behind the environment such as balcony such as office, house, and can prolong at relatively lower temp and following florescence of photoenvironment.
2, be applied to paulownia Developmental Biology and crossbreeding research
The most kinds of the paulownia planted in the field enter the reproductive age and generally take 3～5 years, and a few species will arrive more than 6 years.Research brings many inconvenience and difficulty for the paulownia genetic breeding for this, has not only increased breeding cycle, has also dwindled the scope of genetic manipulation.Adopt the inventive method that the livings seedling of paulownia 1-2 just can be bloomed to pollinate and finish insemination, and obtain ripe viable seed, also make artificial pollination handle and operate more power just and be easy to control, this can make the source of the material of paulownia crossbreeding produce qualitative leap, and can the considerable development that promotes the research of paulownia Developmental Biology.
Claims (4)
1, a kind of flower-forcing method of paulownia seedling may further comprise the steps:
1) cultivate detoxification paulownia tissue cultivating seedling according to the conventional organization culture technique,
2) will carry out culture of rootage after the detoxification paulownia tissue cultivating seedling propagation,
3) be transferred to diameter after nontoxic seedling rooting is cultivated and be 8～30 centimetres, height and be in 8～30 centimetres the flowerpot, in the greenhouse, cultivate more than 40 days, condition of culture is in the greenhouse: do not impose any fertilizer, the temperature on greenhouse daytime is controlled in 25～30 ℃ of scopes, and summer, daylight strength control in greenhouse was in 3000～6000 lux scopes.
2, according to the flower-forcing method of claim 1, it is characterized in that step 2) in carry out culture of rootage condition be: medium is MS+IBA 0.1～0.3mg/L+NAA 0.1～0.3mg/L; Cultivation temperature is 25～28 ℃.
3, according to the flower-forcing method of claim 1, it is characterized in that step 2) in carry out culture of rootage after breeding detoxification paulownia tissue cultivating seedling more than 3 generations.
4, according to the flower-forcing method of claim 1, it is characterized in that nontoxic seedling rooting in the step 3) cultivating the back, to be transferred to diameter after 10～20 days be that 8～10 centimetres, height are in 8～10 centimetres the flowerpot, to cultivate in the greenhouse.
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| CNB2005100645820A CN1327760C (en) | 2005-04-15 | 2005-04-15 | Flower-forcing method for fortunes paulownla seedling |
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| CNB2005100645820A CN1327760C (en) | 2005-04-15 | 2005-04-15 | Flower-forcing method for fortunes paulownla seedling |
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| CN1663351A true CN1663351A (en) | 2005-09-07 |
| CN1327760C CN1327760C (en) | 2007-07-25 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100425122C (en) * | 2006-08-29 | 2008-10-15 | 四川大学 | Method for hastening blossom out in early spring for enjoying flower and orchard |
| CN105104210A (en) * | 2015-09-30 | 2015-12-02 | 江苏农林职业技术学院 | Method for efficiently and rapidly reproducing paulownia test-tube plantlets in large scale |
| CN106489734A (en) * | 2016-10-25 | 2017-03-15 | 广西壮族自治区中国科学院广西植物研究所 | The breeding method of the nontoxic seedling of fortune paulownia |
-
2005
- 2005-04-15 CN CNB2005100645820A patent/CN1327760C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100425122C (en) * | 2006-08-29 | 2008-10-15 | 四川大学 | Method for hastening blossom out in early spring for enjoying flower and orchard |
| CN105104210A (en) * | 2015-09-30 | 2015-12-02 | 江苏农林职业技术学院 | Method for efficiently and rapidly reproducing paulownia test-tube plantlets in large scale |
| CN105104210B (en) * | 2015-09-30 | 2018-07-17 | 江苏农林职业技术学院 | A kind of method that paulownia test tube seedling is efficiently quickly bred in scale |
| CN106489734A (en) * | 2016-10-25 | 2017-03-15 | 广西壮族自治区中国科学院广西植物研究所 | The breeding method of the nontoxic seedling of fortune paulownia |
| CN106489734B (en) * | 2016-10-25 | 2018-03-13 | 广西壮族自治区中国科学院广西植物研究所 | The breeding method of the nontoxic seedling of fortune paulownia |
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| CN1327760C (en) | 2007-07-25 |
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