CN104365468B - Porphyra yezoensis Ueda shell conchocelis stereoscopic culture system and cultural method thereof - Google Patents
Porphyra yezoensis Ueda shell conchocelis stereoscopic culture system and cultural method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of Porphyra yezoensis Ueda shell conchocelis stereoscopic culture system and cultural method thereof, system includes multiple culture tank, and multiple culture tank solids are distributed；Culture tank is arranged with aqua storage tank；Aqua storage tank is introduced the water in culture tank by water circulation system；Culture tank is additionally provided with the artificial light source cultivated for shell conchocelis.Method includes the regulation and control of light, the blue light based on 400～500nm and the HONGGUANG of 600～700nm, is aided with other spectral line light source；Wherein, red blue light strength ratio adjusts between 4: 1～12: 1；Intensity control is at 500Lux 5000Lux；Light application time every day of described artificial light source adjusted between 8 18 hours.The present invention can improve the reliability that nursery produces, and shortens the cultivation cycle of shell conchocelis, and flexible arrangement conchospore is collected seedling the time, and production cost is greatly lowered.
Description
Technical field
The present invention relates to Thallus Porphyrae shell conchocelis culture technique field, particularly relate to a kind of Porphyra yezoensis Ueda shell conchocelis three-dimensional
Culture systems and cultural method thereof.
Background technology
The cultivation of Thallus Porphyrae shell conchocelis is the major technique intension of Thallus Porphyrae nursery engineering, and prior art relies on cement pit planar structure
Condition, uses and open changes Aquaponic pattern.
The existing step of Porphyra yezoensis Ueda seedling-raising technique is: the annual the first tenday period of a month in mid-April to May, by the carpospore of ripe Thallus Porphyrae (or
Person's free conchocelis pulverizes fragment) it is seeded on the shell being laid in cement pit, (need through the cultivations of about 5 months
Suitable sea conditions and light and temperature condition), the nacreous layer of shell gradually overgrows with filamentous, in the stimulation of the conditions such as temperature
Under, fully-developed filamentous discharges conchospore in water body and adheres to lace curtaining, becomes " seedling " of the cultivation of Thallus Porphyrae sea.
The existing technology mode of Thallus Porphyrae nursery is commonly called as industrial seedling rearing, but in essence or half batch production half pesticide herd purification nursery,
Although because existing pattern has used some batch production means to take part in the rings such as temperature in seedling raising process, light intensity and nutritive salt thereof
The regulation of the border factor, but diffuse in the key links such as the time of collecting seedling at carpospore shell inoculation time, conchospore, all can not take off
From the dependence to natural climate condition, seedling raising process can not be broken away from the dependence to nature seawater condition.
The primary condition that prior art is relied on is open cement pit, only is not enough to solve calmly control by means such as ventilation, sunshades
Whole issue in terms of Wen, its weak link shows two aspects: one is that summer temperature higher time shell conchocelis is easy
There is disease；Two is the conchospore mature period such as cold air earlier, easily causes conchospore and diffuses in advance and (diffuse the time
Misfit with the lace curtaining time of plunging into the commercial sea, be commonly called as " race Seedling ").The Factory Building the most spacious many windows the most supporting with open cement pit,
Heat insulating ability is poor, uses the cooling of increase equipment or the efficiency heated is low, cost is high, can not be connect by most producers at present
It is subject to.The reliability that these weak links cause nursery to produce is the highest, and the producer of identical nursery scale is normal in " Seedling net " yield
Often present the difference risen one after another.
Carpospore (or filamentous) inoculation and conchospore are collected seedling and are in the key technology at Thallus Porphyrae seedling raising process two ends, to temperature
Sensitivity is the strongest.Because open cement pit pattern is difficult to break away from the dependence to natural climate condition, prior art time
Significant limitation is had in selection, typically can only be in carpospore inoculation in the first tenday period of a month in annual mid-April to May (or filamentous), JIUYUE
The last ten-days period to early October conchospore is collected seedling.This natural limitation one side that is limited by defines longer cultivation cycle,
On the other hand the most usually occurring that conchospore time of collecting seedling conflicted mutually with the opportunity of suitably plunging into the commercial sea of Seedling net, major part producer needs increase
" cold preservation " link is with the time difference between linking Thallus Porphyrae nursery and marine cultivation, and this both increases management cost and production risk.
Explanation of nouns:
Carpospore: the trophocyte on Thallus Porphyrae frond can form carpospore and anthreid device, and the anthreid device after maturation diffuses
Going out sperm, flow with sea water, run into the receptive hypha of carpogonium, sperm enters carpogonium fertilization, forms zygote, and zygote is through repeatedly dividing
It is cleaved into as carpospore.Carpospore is circular, diameter 8um-11um.
Thallus Porphyrae shell conchocelis: released carpospore after Thallus Porphyrae maturation, the contact substrate (various shell) containing calcium carbonate is just
Attachment, sprout and pierce shell in be grown to elongated irregular filamentous, diameter is generally 2um-4um.
Conchosporangia: thread algal filament grows to certain phase, forms the relatively rough segmentation being made up of column type cell and props up, and diameter can
Reach 8um～16um.Immature conchosporangia cell length-width ratio is about 2～3 times, and gradually ripe conchosporangia is thin
Born of the same parents' length and width tend to equal.
Conchospore: autumn, water temperature began to decline, shell conchocelis during the conchosporangia full maturity grown, cell
Being divided into two, now cell cross wall runs off, and branch becomes tubulose, and conchospore is discharged from the mouth of pipe.Conchospore drifts about with sea water, short
Time catch cropping amoeboid movement after, be attached in substrate and sprout.
Summary of the invention
The technical problem to be solved is to provide a kind of Porphyra yezoensis Ueda shell conchocelis stereoscopic culture system and cultivation thereof
Method, can improve the reliability that nursery produces, and shortens the cultivation cycle of shell conchocelis, when flexible arrangement conchospore is collected seedling
Between, production cost is greatly lowered.
The technical solution adopted for the present invention to solve the technical problems is: provide a kind of Porphyra yezoensis Ueda shell conchocelis stereoscopic culture
System, including multiple culture tanks, the plurality of culture tank solid is distributed；Described culture tank is arranged with aqua storage tank；Described storage
Tank is introduced the water in described culture tank by water circulation system；Described culture tank is additionally provided with for shell conchocelis cultivation
Artificial light source；Described artificial light source based on 400～500nm blue light and the HONGGUANG of 600～700nm, other spectral lines
Visible light source is auxiliary.
Being provided with sea water air conditioner in described aqua storage tank, described sea water air conditioner is for carrying out water temperature in the shell conchocelis nurturing period
Control.
The technical solution adopted for the present invention to solve the technical problems is: also provide for a kind of Porphyra yezoensis Ueda shell conchocelis solid training
Breeding method, uses above-mentioned Porphyra yezoensis Ueda shell conchocelis stereoscopic culture system, including the regulation and control of light, with 400～500nm
Blue light and 600～700nm HONGGUANG be main, be aided with the visible light source of other spectral line；Wherein, red blue light strength ratio
Adjust between 4: 1～12: 1；Intensity control is at 500Lux-5000Lux；Light application time every day of described artificial light source exists
Adjust between 8-18 hour.
On the premise of the temperature control that do not conflicts, it is combined with lamp and realizes the regulation and control of light.
Described Porphyra yezoensis Ueda shell conchocelis stereoscopic culture method also includes the regulation and control of water, need according to the growth of different phase and
For the time requirement of Seedling, 14 DEG C-28 DEG C interval adjustment.
According to the weather conditions of culture period during water regulation and control, it is combined with natural temperature, starts sea water air conditioner and heat or drop
Temperature.
Water in described aqua storage tank is artificial seawater or through disinfecting nature seawater；Described artificial seawater or nature seawater are fixed
Phase supplements the inorganic fertilizer based on nitrogen phosphorus.
It is set at HONGGUANG described in different developmental phases and blue light ratio and light intensity: carpospore sprouts the stage: red blue light ratio is
4: 1～7: 1, intensity control is at 3000Lux-4000Lux；The thread algal filament stage: red blue light ratio is 6: 1～10: 1,
Intensity control is at 2000Lux-3000Lux；The conchosporangia stage: red blue light ratio is 6: 1～10: 1, intensity control
At 1000Lux-2000Lux；Conchospore formation stages: red blue light ratio is 8: 1～12: 1, and intensity control exists
500Lux-1000Lux；Conchospore diffuses the stage: red blue light ratio is 8: 1～12: 1, and intensity control exists
4000Lux-5000Lux。
It is set in different developmental phases water temperature: carpospore sprouts the stage: 14 DEG C-22 DEG C；The thread algal filament stage: 22 DEG C-25 DEG C；
The conchosporangia stage: 25 DEG C-28 DEG C；Conchospore formation stages: 24 DEG C-26 DEG C；Conchospore diffuses the stage: 20 DEG C-23 DEG C.
Beneficial effect
Owing to have employed above-mentioned technical scheme, the present invention compared with prior art, has the following advantages that and good effect:
The present invention puts forth effort on and builds intensive stereoscopic culture pattern so that intelligent temperature control light-control technology and water circulation use skill
The embedding of art is possibly realized, and to improving the reliability that nursery produces, shortens the cultivation cycle of shell conchocelis, flexible arrangement
Conchospore is collected seedling the time, and production cost is greatly lowered.
The present invention, according to the needs of shell conchocelis growth promoter, is preset in the temperature treatment program closed under condition of culture, borrows
Help the facilities such as intelligent air conditioner to carry out program control management to cultivating water temperature, eliminate the reliance on natural climate condition.Thread according to shell
The needs that bulk-growth is grown, are preset in the light quality of artificial light source, light intensity and photoperiod combinator under closing condition of culture, borrow
Help the facility such as LED and Intelligent fixed time switch and carry out program control management to cultivating illumination, no longer major regulatory nature light.This
Bright to cultivate shell conchocelis be circulated utilization with water, including artificial seawater preparation, water quality monitoring, nutritive salt, trace
Element and carbon source such as supplement at the supporting technology, eliminate the reliance on nature seawater.By said temperature control, illumination control, water quality control
System and the Integration ofTechnology such as recycle, the present invention realizes three-dimensional and arranges shell at many levels.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is expanded on further.Should be understood that these embodiments be merely to illustrate the present invention and
It is not used in restriction the scope of the present invention.In addition, it is to be understood that after having read the content that the present invention lectures, people in the art
The present invention can be made various changes or modifications by member, and these equivalent form of values fall within the application appended claims equally and limited
Scope.
The present invention relates to a kind of Porphyra yezoensis Ueda shell conchocelis stereoscopic culture system, as it is shown in figure 1, include multiple culture tank 1,
The three-dimensional distribution of the plurality of culture tank 1；Described culture tank 1 is arranged with aqua storage tank 2；Described aqua storage tank 2 is circulated by water
System 3 introduces the water in described culture tank 1；Described culture tank 1 is additionally provided with the artificial light source cultivated for shell conchocelis
2；Described artificial light source 2 based on 400～500nm blue light and the HONGGUANG of 600～700nm, other spectral lines visible
Light source is auxiliary.Wherein, being provided with sea water air conditioner in described aqua storage tank 2, described sea water air conditioner is for training at shell conchocelis
Water temperature control is carried out during educating.
Shell is inoculated
Inoculation adherance can be with biologies such as fresh Conch Meretricis seu Cyclinae shell, Concha Ostreae or other suitable substitute materials, Conch Meretricis seu Cyclinae shell and Concha Ostreaes
Material should fully soak and wash；Adherance is neatly emitted in the culture tank of three-dimensional distribution, injects disappearing of about 10cm
Poison sea water or artificial seawater (proportion 1.017-1.021, PH7.8-8.3)；System is arranged: light intensity 1500Lux-3000Lux,
Temperature 15 DEG C-20 DEG C.
Carpospore inoculation is pressed the standard of DB32/T162 2002 and is selected kind of an algae；Kind of algae sea water cleans, be dehydrated, dry in the shade rearmounted
Entering refrigerator-freezer and store standby (moisture content about 20-30%), temperature controls below-15 DEG C；During inoculation, the kind algae dried in the shade is directly placed into
Fill in the container of clean sea water and diffuse, should constantly stir sea water during diffusing, and often draw water sample microscopy carpogonium
The amount of diffusing of son and quality；After the carpospore amount in water body reaches certain density, take out kind of an algae, by bolting silk or filtered through gauze
Spore liquid；The density that carpospore is rendered on adherance is advisable with 150-300/cm2；Spore liquid is the most uniformly sprayed at
On adherance in culture tank.
Filamentous inoculation is carried out by DB/T168 standard-required.
Check that the sprouting amount on adherance is preferably carried out after inoculation for 10-20 days, sprouting amount with 40-60 algae fall/cm2 is advisable,
Sprouting amount is too low can be with filling mining.
Shell is cultivated
The regulation and control of light: the artificial light source cultivated for shell conchocelis, the blue light with 400～500nm and 600～700nm
HONGGUANG be main, be aided with other spectral line light source.Wherein, red blue light strength ratio according to different developmental phases 4: 1～12:
Adjusting between 1, light intensity is at 500Lux-5000Lux；On the premise of the temperature control that do not conflicts, lamp can be combined with,
Sunshine-duration adjusted between 8～18 hours.Different developmental phases red blue light ratio and light intensity arrange and are shown in Table 1:
| Stage of development | Carpospore sprouts | Thread algal filament | Conchosporangia | Conchospore is formed | Conchospore diffuses |
| Red blue light ratio | 4:1～7: 1 | 6:1～10: 1 | 6:1～10: 1 | 8:1～12: 1 | 8:1～12: 1 |
| Light intensity | 3000～4000 | 2000～3000 | 1000～2000 | 500～1000 | 4000～5000 |
Table 1
The regulation and control of water: the water temperature of shell conchocelis nurturing period controls, growing needs and supplying the time of Seedling according to different phase
Requirement, 14 DEG C-28 DEG C interval adjustment；According to the weather conditions of culture period, natural temperature can be combined with, if desired
Start sea water air conditioner heat or lower the temperature；Nature seawater regular replenishment must be sought with other based on the inorganic fertilizer of nitrogen phosphorus
Form point.The nutrient formulation of artificial seawater suitably adjusts on the basis of PES；The nature seawater supplemented has to pass through at sterilization
Reason.Different developmental phases water temperature arranges and is shown in Table 2:
| Stage of development | Carpospore sprouts | Thread algal filament | Conchosporangia | Conchospore is formed | Conchospore diffuses |
| Water temperature DEG C | 14 DEG C～22 DEG C | 22 DEG C～25 DEG C | 25 DEG C～28 DEG C | 24 DEG C～26 DEG C | 20 DEG C～23 DEG C |
Table 2
Other management: note down the change curve of the envirment factors such as water temperature, pH value and illumination, periodic detection sea water specific gravity online
With indexs such as nutritive salt；Depending on the miscellaneous algae of shell, a situation arises, scrubs adherance if desired；Use freshwater soaking adherance if desired
Filamentous disease can be prevented and treated；Periodically carry out filamentous developmental state inspection, record algal filament distribution and color and luster situation of change, shell
The T/A that sporangial branchlets occurs, the T/A that conchospore is formed.
Shell is collected seedling
Collect seedling preparation: assist client by DB32/T170 standard preparation lace curtaining；Change fresh seawater, increase flow has necessarily
Accelerating effect；Can collect seedling when the conchospore on adherance diffuses and reaches some；Routine time of collecting seedling typically exists
Late September to early October is carried out, it is possible to use common nursery pond traditionally operates；The unconventional time of collecting seedling collects seedling
Need to carry out in specific temperature control workshop, operation uses dilution spore liquid circulated sprinkling.
Collect seedling method: operation of collecting seedling typically is carried out in the morning；Culture tank light intensity is adjusted to more than 3000Lux；Follow bad discharge to increase
Big and start flushing system；Spore liquid is removed and changes water and continues cycling through bath by separated in time (depending on spore density)；
During spray, lace curtaining can stir.
Spatfall density: spatfall density of often taking a sample to check during collecting seedling, should go out pond in time when touching the mark；Use vinylon
Monofilament dissipates head inspection, is advisable adhering to 50-80/cm conchospore；Use nettle inspection, to adhere to 10-15/visual field
(10 × 10) conchospore is advisable；The Seedling net hanged up of can not plunging into the commercial sea in time should be deposited in ventilating and cooling place, and sprays sea water holding
Moistening, or Seedling net is placed with in the storage pond, holding pond of sea water supports temporarily.
It is seen that, the Thallus Porphyrae nursery engineering implemented in open cement pit is transplanted in airtight, semi-hermetic by the present invention first
Formula culture systems is carried out, and solves the light of filamentous artificial light source in stereoscopic culture system required for growth promoter
Matter, light intensity and the key technical problem such as photoperiod combination and recirculated water cultivation.Technical solution of the present invention is main with prior art
Difference see table.
As can be seen here, the technology of the present invention is put forth effort on and is built intensive stereoscopic culture pattern so that intelligent temperature control light-control technology
And the embedding of water circulation use technology is possibly realized, to improving the reliability that nursery produces, shorten the cultivation of shell conchocelis
In the cycle, flexible arrangement conchospore is collected seedling the time, and production cost is greatly lowered.Intensive stereoscopic culture pattern, Ke Yigui
Belong to for intelligentized factory agricultural.
Claims (7)
1. a Porphyra yezoensis Ueda shell conchocelis stereoscopic culture method, it is characterised in that use Porphyra yezoensis Ueda shell conchocelis three-dimensional
Culture systems, described stereoscopic culture system includes multiple culture tank (1), the three-dimensional distribution of described culture tank (1)；Described
Culture tank (1) is arranged with aqua storage tank (2)；Described aqua storage tank (2) is introduced the water into by water circulation system (3)
In described culture tank (1)；Described culture tank (1) is additionally provided with the artificial light source (4) cultivated for shell conchocelis；
The regulation and control of light are included during use, the blue light based on 400～500nm and the HONGGUANG of 600～700nm, it is aided with it
The visible light source of its spectral line；Wherein, red blue light strength ratio adjusts between 4: 1～12: 1；Intensity control exists
500Lux-5000Lux；Light application time every day of described artificial light source adjusted between 8-18 hour；Rank are grown in difference
Duan Suoshu HONGGUANG and blue light ratio and light intensity are set to: carpospore sprouts the stage: red blue light ratio is 4: 1～7: 1,
Intensity control is at 3000Lux-4000Lux；The thread algal filament stage: red blue light ratio is 6: 1～10: 1, intensity control
At 2000Lux-3000Lux；The conchosporangia stage: red blue light ratio is 6: 1～10: 1, and intensity control exists
1000Lux-2000Lux；Conchospore formation stages: red blue light ratio is 8: 1～12: 1, and intensity control exists
500Lux-1000Lux；Conchospore diffuses the stage: red blue light ratio is 8: 1～12: 1, and intensity control exists
4000Lux-5000Lux。
Porphyra yezoensis Ueda shell conchocelis stereoscopic culture method the most according to claim 1, it is characterised in that in the control that do not conflicts
On the premise of temperature, it is combined with lamp and realizes the regulation and control of light.
Porphyra yezoensis Ueda shell conchocelis stereoscopic culture method the most according to claim 1, it is characterised in that also include water
Regulation and control, growing needs and supplying the time requirement of Seedling according to different phase, adjust in 14 DEG C-28 DEG C intervals.
Porphyra yezoensis Ueda shell conchocelis stereoscopic culture method the most according to claim 3, it is characterised in that root during water regulation and control
According to the weather conditions of culture period, it is combined with natural temperature, starts sea water air conditioner and heat or lower the temperature.
Porphyra yezoensis Ueda shell conchocelis stereoscopic culture method the most according to claim 1, it is characterised in that described aqua storage tank
In water be artificial seawater or through disinfecting nature seawater；Described artificial seawater or nature seawater regular replenishment are with nitrogen
Phosphorus is main inorganic fertilizer.
Porphyra yezoensis Ueda shell conchocelis stereoscopic culture method the most according to claim 1, it is characterised in that described aqua storage tank
(2) being provided with sea water air conditioner in, described sea water air conditioner is for carrying out water temperature control in the shell conchocelis nurturing period.
Porphyra yezoensis Ueda shell conchocelis stereoscopic culture method the most according to claim 3, it is characterised in that grow in difference
Stage water temperature is set to: carpospore sprouts the stage: 14 DEG C-22 DEG C；The thread algal filament stage: 22 DEG C-25 DEG C；Conchospore
The branch stage: 25 DEG C-28 DEG C；Conchospore formation stages: 24 DEG C-26 DEG C；Conchospore diffuses the stage: 20 DEG C-23 DEG C.
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| CN104855268A (en) * | 2015-04-29 | 2015-08-26 | 王继波 | Porphyra seedling culture method and culture pond body |
| CN105145329B (en) * | 2015-09-15 | 2017-07-11 | 宁波太空红水产养殖有限公司 | It is a kind of to promote Conchospores of Porphyra Hait Anensis to concentrate ripe method |
| CN105684880B (en) * | 2016-02-16 | 2020-10-02 | 珀莱雅化妆品股份有限公司 | Culture method capable of improving mycosporine-like amino acid content in umbilical laver |
| CN106489712A (en) * | 2016-10-09 | 2017-03-15 | 盐城海瑞食品有限公司 | The seedling raising process of porphyra haitanensis |
| CN106688869B (en) * | 2016-12-28 | 2020-11-24 | 江苏省海洋水产研究所 | Method for preventing and treating shell filament yellow spot of porphyra yezoensis and culture device |
| CN107410002B (en) * | 2017-04-26 | 2023-01-31 | 浙江省海洋水产养殖研究所 | Algae control method in porphyra haitanensis shell filament cultivation process |
| CN107616088A (en) * | 2017-10-26 | 2018-01-23 | 中国水产科学研究院黄海水产研究所 | The vertical device for raising seedlings of one main laver shell conchocelis and its application method |
| CN107549003B (en) * | 2017-10-26 | 2023-06-02 | 中国水产科学研究院黄海水产研究所 | Kelp three-dimensional seedling raising device and application method thereof |
| CN110367114B (en) * | 2019-08-07 | 2024-03-12 | 浙江省海洋水产养殖研究所 | Porphyra haitanensis hanging shell filament cleaning device |
| CN113854140B (en) * | 2021-11-05 | 2022-10-18 | 江苏省海洋水产研究所 | Modularized porphyra yezoensis seedling three-dimensional culture method |
| CN115380814A (en) * | 2022-09-28 | 2022-11-25 | 中国农业科学院农业环境与可持续发展研究所 | Porphyra yezoensis plant factory production system and method |
| CN118318723B (en) * | 2024-04-25 | 2024-09-27 | 宁波大学 | Industrial laver cultivation system and cultivation method |
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| JPH10178947A (en) * | 1996-12-26 | 1998-07-07 | Sanyo Electric Co Ltd | Device and method for farming seaweeds |
| CN1328950C (en) * | 2004-04-26 | 2007-08-01 | 中国科学院海洋研究所 | Seedling raising method for non-shell protonema cell of laver |
| JP2012016343A (en) * | 2010-07-09 | 2012-01-26 | Akkii Foods:Kk | Method for culturing algal body of brown alga sargassum hurneri |
| CN203492505U (en) * | 2013-07-18 | 2014-03-26 | 林丹凤 | Intelligent light supplementing device of laver seedling culturing room |
| CN104106462B (en) * | 2014-07-04 | 2016-09-14 | 常熟理工学院 | A kind of method of Porphyra yezoensis Ueda breeding application |
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