CN103535328A - Method for industrially breeding phyoseiulus persimilis - Google Patents
Method for industrially breeding phyoseiulus persimilis Download PDFInfo
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- CN103535328A CN103535328A CN201310512139.XA CN201310512139A CN103535328A CN 103535328 A CN103535328 A CN 103535328A CN 201310512139 A CN201310512139 A CN 201310512139A CN 103535328 A CN103535328 A CN 103535328A
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Abstract
The invention relates to a method for industrially breeding phyoseiulus persimilis. The method includes steps of A, constructing an industrial breeding greenhouse, specifically, dividing the greenhouse into a phyoseiulus persimilis food-chain tetranychus urticae breeding area, a tetranychus urticae food-chain clean seedling planting area and a phyoseiulus persimilis isolation breeding area; B, planting clean seedlings, in other words, cultivating peanuts in nutrition bowls, and keeping the temperature of the clean seedling planting area to range from 18 DEG C to 35 DEG C and the humidity of the clean seedling planting area to range from 35% to 85%; C, breeding tetranychus urticae, in other words, breeding a large quantity of tetranychus urticae in the greenhouse and performing conservation breeding on the tetranychus urticae in small climatic chambers synchronously; D, breeding the phyoseiulus persimilis in an isolation manner. The peanuts are selected to be used as food for the tetranychus urticae. The method has the advantages that the method aims to solve the problem that a large quantity of phyoseiulus persimilis cannot be bread all the year round, the phyoseiulus persimilis can be bred by means of cultivating host plants and breeding host insects and natural enemy insects in the glass greenhouse all the year round under full automatic control, so that purposes of low energy consumption, high efficiency and long-term output can be achieved.
Description
Technical field
The present invention relates to useful insect batch production and produce and pest biological prevention field, particularly the method for the little phytoseiid mite of a kind of industrialized propagation Chile.
Background technology
Aggravation along with mankind's activity; the increase of the pressure of grain, population, natural resources; ball ecological environment worsens year by year with unprecedented speed, and survival crisis is deepened day by day, and " economic sustainable development and the sustainable use of resource " policy receives publicity.The strategy of Pest Management also will note preventing the atrophy of natural ecosystems and impaired, to adapt to the needs (Ding Yanqin, 2005) of the mankind, environment long-term interest.At present, evil mite is to harm the crops and the important pests of fruit tree, can endanger the multiclass crops such as eggplant, cotton, strawberry, legume crop, apple, oranges and tangerines, brings massive losses to peasant's production.The Phytoseiulus Persimilis of take evil mite, as main green prevention and control technology, has been subject to the great attention of all circles.
In the little phytoseiid mite production application of artificial breeding Chile, for different facility types, different cultivation season, occurred breeding not in time, the problem such as breeding amount is inadequate, only have and realize high efficiency continuous breeding and could solve problems." propagation method of the little phytoseiid mite of a kind of Chile (patent No. 200810118205.4) " of Beijing City Agriculture and Forestry Institute application, does not relate to workshop, greenhouse and facility condition; Tetranychus urticae greenhouse mass rearing and the breeding of microclimate chamber conservation; The aspects such as Chile's little phytoseiid mite breeding isolation.
Summary of the invention
The object of the invention is to for the little phytoseiid mite of Chile mass rearing problem all the year round, a kind of method of the full automatic control glass greenhouse anniversary cultivating host plant, host egg insect and natural enemy insect of utilizing is provided, realize low energy consumption, high efficiency, output all the year round.
The little phytoseiid mite method of this industrialized propagation Chile, realizes as follows:
Greenhouse is bred in A, construction batch production: greenhouse is divided into Chilean little phytoseiid mite food chain Tetranychus urticae multiplication plot, and Tetranychus urticae food chain cleans seedling growing area, and Chilean little phytoseiid mite isolation multiplication plot;
B, clean seed are planted: selectively beans, as the food of Tetranychus urticae, are cultivated in nutritive cube, 18-35 ℃ of clean seedling growing area temperature, humidity 35%-85%;
C, Tetranychus urticae are bred: synchronously carry out Tetranychus urticae greenhouse mass rearing and the breeding of microclimate chamber conservation, and part mass rearing in greenhouse, another part is bred in microclimate chamber conservation; Microclimate chamber remains the little phytoseiid mite breeding of purer Chile, in order to regular replenishment greenhouse Tetranychus urticae propagating population;
D, Chilean little phytoseiid mite isolation breeding: Chilean little phytoseiid mite plant propagation greenhouse independently becomes a greenhouse with clean seedling district separately with greenhouse, tetranychid district, and the 20 meters of above distances of being separated by, and avoids Chilean little phytoseiid mite to import Tetranychus urticae multiplication plot into, affects tetranychid and normally breeds.
The described little phytoseiid mite method of industrialized propagation Chile, greenhouse described in steps A and subregion all have strict seal, greenhouse arranges roof window ventilating opening, 80 order fly nets are all installed on each ventilating opening and both sides, corridor, cooling adopts " wet curtain and blower fan " combination and air-conditioning, heats and adopts circular wing type heating installation and air-conditioning.
The little phytoseiid mite method of described industrialized propagation Chile, greenhouse and subregion cooling minute four grades described in steps A, 1. ambient temperature 25-28 ℃ time, only open roof window gravity-flow ventilation; 2. extraneous 29-32 ℃ time, drive roof window and blower fan; 3. ambient temperature 33-35 ℃ time, drive wet curtain and blower fan; When 4. ambient temperature is more than 36 ℃, turn on the aircondition; Heat up to adopt heating installation, if temperature during still lower than 18 ℃, the auxiliary heating of turning on the aircondition.
The little phytoseiid mite method of described industrialized propagation Chile, clean bean seedlings plantation described in step B comprises, potting soil is put into the nutritive cube of medium size 16cm * 14cm, in each nutritive cube, 3 ground beans seeds of plantation, put the ground beans of having planted on seedbed in order, plug automatic drip irrigation watering facility, every basin is inserted a drip irrigation pipe, water every three days water one time, note watering the water yield and should not too much, keep soil from packing together; After emerging, every about 10 days, water chemical fertilizer water one time, strengthen management, cultivate healthy and strong bean seedlings.
The little phytoseiid mite method of described industrialized propagation Chile, Tetranychus urticae greenhouse mass rearing described in step C is used for expanding numerous and inoculates Chilean little phytoseiid mite, method comprises, the potted plant fresh healthy and strong bean seedlings of a part are moved into Tetranychus urticae and breed workshop, every basin inoculation 3-4 sheet is with the blade of tetranychid, on every leaf, the amount of Tetranychus urticae is 50-100 head, when Tetranychus urticae is transferred on new blade, clears up in time carrier blade; Temperature 18-32 ℃ of multiplication plot, Tetranychus urticae greenhouse, humidity 35%-75%.
The little phytoseiid mite method of described industrialized propagation Chile, microclimate chamber conservation breeding Tetranychus urticae described in step C, adopts the breeding of bean seedlings inoculation Tetranychus urticae, after 5-7 days, partial blade is cut as inoculation carrier inoculation new talent, the remaining greenhouse of bringing into, supplements its Tetranychus urticae propagating population.
The little phytoseiid mite method of described industrialized propagation Chile, described microclimate chamber surrounding is that glass curtain wall is beneficial to solar radiation, and external door, inside door isolation are set, inner air-conditioning, light compensating lamp, the dehumidifier of being equipped with, by automatic controlling system, temperature 20-31 ℃, humidity 35%-55%.
The little phytoseiid mite method of described industrialized propagation Chile, Chile's little phytoseiid mite isolation breeding described in step D comprises: when the amount of every basin bean seedlings Tetranychus urticae is 800-1000, move the Chilean little phytoseiid mite chamber of breeding inoculation Predatory Mites to, select the plant leaf that contains the little phytoseiid mite of a large amount of Chile, with scissors, cut gently, be placed in pallet as inoculation carrier, then with tweezers gently gripping blade be placed directly on bean seedlings and inoculate, the little phytoseiid mite of Chile can be climbed to very soon on bean seedlings and take food Tetranychus urticae, and inoculative proportion is that 1:40-50 is advisable.
The little phytoseiid mite method of described industrialized propagation Chile, in the little phytoseiid mite isolation of Chile multiplication plot, for preventing Chilean little phytoseiid mite, run away, breeding of the little phytoseiid mite of Chile adopts movable seedbed to place, in rectangle tank central authorities, " one " word is arranged, apart from tank dual-side edge, be 35cm, water logging in anti-water stopping trough there is not plant, and potted plant heelpiece goes out the water surface; When 40,000 of Chilean little phytoseiid mites of every day breeding are above, can meet 10-30 mu facilities vegetable tetranychid biological control demand.
Technological progress effect of the present invention shows:
(1) clear and definite greenhouse and appointed condition of breeding Chilean little phytoseiid mite, adopts " cascade+blower fan " combined type to substitute air-conditioning temperature-reducing, greatly reduces energy resource consumption; Each link all has strict quarantine measures, has solved different subregion insects and has mutually fled interference problem.
(2) synchronously carry out Tetranychus urticae greenhouse mass rearing and the breeding of microclimate chamber conservation, phytotron can keep purer Tetranychus urticae all the time, in order to supplementing greenhouse Tetranychus urticae propagating population, has improved the annual quantity-produced stability of Tetranychus urticae.
(3) the cultured space of Chilean little phytoseiid mite is comparatively independent, strictly separates with the long distance in the Tetranychus urticae chamber of breeding, and prevents that two kinds of insects from running away mutually, affects feeding efficiency.
Accompanying drawing explanation
Fig. 1 is the plane figure schematic diagram of greenhouse subregion
Fig. 2 is Chilean little phytoseiid mite propagation tank floor map
In figure:
1, clean seedling district 2, Tetranychus urticae multiplication plot 3, corridor 4, cascade 5 blower fans
6, Chilean little phytoseiid mite multiplication plot 7, isolation strip 6-1, Chilean little phytoseiid mite greenhouse
6-2, movable seedbed 6-3, rectangle tank 6-4, potted plant seedling
Embodiment
The prebiotic anti-Co., Ltd in Hengshui City field in September, 2009 to the 2013 year little phytoseiid mite industrialized propagation of Chile in September of take below further illustrates as embodiment:
Tetranychus urticae (greenhouse, Hengshui City Taocheng District, Hebei province gathers, host's eggplant)
Chile's little phytoseiid mite (Beijing City Academy Of Agriculture and Forest Sciences INST OF PLANT PROT & ENVIRONME provides)
1, workshop, greenhouse and facility condition: as shown in Figure 1, workshop, greenhouse is divided into clean seedling and cultivates district 1, Tetranychus urticae multiplication plot 2, Chilean little phytoseiid mite multiplication plot 6 three parts.At same district intersection not, increase glass partition, cascade 4 heat sinks and fly net are installed in both sides, corridor, the blower fan 5 that configuration is ventilated and cooling, heating device.Greenhouse and subregion all have strict seal, and greenhouse arranges roof window ventilating opening, and 80 order fly nets are all installed on each ventilating opening and both sides, corridor, and cooling adopts " wet curtain and blower fan " combination and air-conditioning, heats and adopts circular wing type heating installation and air-conditioning.
Greenhouse and subregion cooling minute four grades, 1. ambient temperature 25-28 ℃ time, only open roof window gravity-flow ventilation; 2. extraneous 29-32 ℃ time, drive roof window and blower fan; 3. ambient temperature 33-35 ℃ time, drive wet curtain and blower fan; When 4. ambient temperature is more than 36 ℃, turn on the aircondition; Heat up to adopt heating installation, if temperature during still lower than 18 ℃, the auxiliary heating of turning on the aircondition.
2, clean seed is planted: selectively beans, as the food of Tetranychus urticae, are cultivated in nutritive cube, 18-35 ℃ of clean seedling growing area temperature, humidity 35%-85%;
Every day, potted plant 65 basin bean seedlings, put into medium size nutritive cube (16cm * 14cm) by potting soil, 3 ground beans seeds of plantation in each nutritive cube.The ground beans of having planted are put in order on seedbed, plugged automatic drip irrigation watering facility, every basin is inserted a drip irrigation pipe, waters every three days water one time, notes watering the water yield and should not too much, keep soil from packing together; After emerging, every about 10 days, water chemical fertilizer water one time, strengthen management, cultivate healthy and strong bean seedlings.
3, Tetranychus urticae is bred: synchronously carry out Tetranychus urticae greenhouse mass rearing and the breeding of microclimate chamber conservation, and part mass rearing in greenhouse, another part is bred in microclimate chamber conservation; Inferior climatic chamber remains purer Tetranychus urticae, in order to regular replenishment greenhouse Tetranychus urticae propagating population;
In 3-1, greenhouse, the method for mass rearing Tetranychus urticae is: the potted plant bean seedlings of picking every day after pinching carry out mass rearing Tetranychus urticae in greenhouse.Method is that plant that picking is loaded with Tetranychus urticae 50-100 head/leaf is moved Chilean little phytoseiid mite to and breeds district 2, clip is placed in plate with 200 left and right of blade (50-100 head/leaf) of tetranychid, with tweezers gripping blade gently, be placed on fresh bean seedlings, every basin is put 3-4 sheet leaf.
In order to be conducive to Tetranychus urticae, breed, preferably controlling temperature is 20-31 ℃, humidity 35%-55%, and temperature is during higher or lower than design temperature, and computer automatically starts or closes air discharging facility.
3-2, the breeding of microclimate chamber conservation Tetranychus urticae, adopt bean seedlings inoculation after 5-7 days, partial blade to be cut as inoculation carrier inoculation new talent, and the remaining greenhouse of bringing into, supplements its Tetranychus urticae propagating population.
Microclimate chamber surrounding is that glass curtain wall is beneficial to solar radiation, and external door, inside door isolation are set, inner air-conditioning, light compensating lamp, the dehumidifier of being equipped with, and by automatic controlling system, temperature 20-31 ℃, humidity 35%-55%.
4, Chilean little phytoseiid mite batch production is bred in enormous quantities:
When the amount of every basin bean seedlings Tetranychus urticae is 800-1000, move the Chilean little phytoseiid mite chamber of breeding inoculation Predatory Mites-Chilean little phytoseiid mite to, select the plant leaf that contains the little phytoseiid mite of a large amount of Chile, with scissors, cut gently, be placed in pallet as inoculation carrier, then with tweezers gently gripping blade be placed directly on bean seedlings and inoculate, Chilean little phytoseiid mite can be climbed to very soon on bean seedlings and take food Tetranychus urticae, inoculative proportion is that 1:40-50 is advisable.
For preventing that Chilean little phytoseiid mite from running away, Chilean little phytoseiid mite plant propagation greenhouse independently becomes a greenhouse with clean seedling district separately with greenhouse, tetranychid district, and the 20 meters of above distances of being separated by, and avoids Chilean little phytoseiid mite to import Tetranychus urticae multiplication plot into, affects tetranychid and normally breeds.
As shown in Figure 2, the multiplication plot 6 of Chilean little phytoseiid mite is bred and is adopted movable seedbed 2 to place, and in rectangle tank 3 central authorities " ", word is arranged, and apart from tank dual-side, along being 35cm, the water logging in anti-water stopping trough there is not plant, and potted plant heelpiece goes out the water surface; When 40,000 of Chilean little phytoseiid mites of every day breeding are above, can meet 10-30 mu facilities vegetable tetranychid biological control demand.
Claims (9)
1. the little phytoseiid mite method of industrialized propagation Chile, its feature comprises the steps:
Greenhouse is bred in A, construction batch production: greenhouse is divided into Chilean little phytoseiid mite food chain Tetranychus urticae multiplication plot, and Tetranychus urticae food chain cleans seedling growing area, and Chilean little phytoseiid mite isolation multiplication plot;
B, clean seed are planted: selectively beans, as the food of Tetranychus urticae, are cultivated in nutritive cube, 18-35 ℃ of clean seedling growing area temperature, humidity 35%-85%;
C, Tetranychus urticae are bred: synchronously carry out Tetranychus urticae greenhouse mass rearing and the breeding of microclimate chamber conservation, and part mass rearing in greenhouse, another part is bred in microclimate chamber conservation; Microclimate chamber remains purer Tetranychus urticae, in order to regular replenishment greenhouse Tetranychus urticae propagating population;
D, Chilean little phytoseiid mite isolation breeding: Chilean little phytoseiid mite plant propagation greenhouse independently becomes a greenhouse with clean seedling district separately with greenhouse, tetranychid district, and the 20 meters of above distances of being separated by, and avoids Chilean little phytoseiid mite to import Tetranychus urticae multiplication plot into, affects tetranychid and normally breeds.
2. the little phytoseiid mite method of industrialized propagation according to claim 1 Chile, it is characterized in that: the greenhouse described in steps A and subregion all have strict seal, greenhouse arranges roof window ventilating opening, 80 order fly nets are all installed on each ventilating opening and both sides, corridor, cooling adopts " wet curtain and blower fan " combination and air-conditioning, heats and adopts circular wing type heating installation and air-conditioning.
3. the little phytoseiid mite method of industrialized propagation according to claim 2 Chile, is characterized in that: the greenhouse described in steps A and subregion cooling minute four grades, 1. ambient temperature 25-28 ℃ time, only open roof window gravity-flow ventilation; 2. extraneous 29-32 ℃ time, drive roof window and blower fan; 3. ambient temperature 33-35 ℃ time, drive wet curtain and blower fan; When 4. ambient temperature is more than 36 ℃, turn on the aircondition; Heat up to adopt heating installation, if temperature during still lower than 18 ℃, the auxiliary heating of turning on the aircondition.
4. the little phytoseiid mite method of industrialized propagation Chile according to claim 1, it is characterized in that: the clean bean seedlings plantation described in step B comprises, potting soil is put into the nutritive cube of medium size 16cm * 14cm, in each nutritive cube, 3 ground beans seeds of plantation, put the ground beans of having planted on seedbed in order, plug automatic drip irrigation watering facility, every basin is inserted a drip irrigation pipe, water every three days water one time, note watering the water yield and should not too much, keep soil from packing together; After emerging, every about 10 days, water chemical fertilizer water one time, strengthen management, cultivate healthy and strong bean seedlings.
5. the little phytoseiid mite method of industrialized propagation Chile according to claim 1, it is characterized in that: the Tetranychus urticae greenhouse mass rearing described in step C is used for expanding numerous and inoculates Chilean little phytoseiid mite, method comprises, the potted plant fresh healthy and strong bean seedlings of a part are moved into Tetranychus urticae and breed workshop, every basin inoculation 3-4 sheet is with the blade of tetranychid, on every leaf, the amount of Tetranychus urticae is 50-100 head, when Tetranychus urticae is transferred on new blade, clears up in time carrier blade; Temperature 18-32 ℃ of multiplication plot, Tetranychus urticae greenhouse, humidity 35%-75%.
6. the little phytoseiid mite method of industrialized propagation Chile according to claim 1, it is characterized in that: microclimate chamber conservation breeding Tetranychus urticae described in step C, adopt the breeding of bean seedlings inoculation Tetranychus urticae, after 5-7 days, partial blade is cut as inoculation carrier inoculation new talent, the remaining greenhouse of bringing into, supplements its Tetranychus urticae propagating population.
7. the little phytoseiid mite method of industrialized propagation Chile according to claim 6, it is characterized in that: described in step C, microclimate chamber surrounding is that glass curtain wall is beneficial to solar radiation, external door, inside door isolation are set, inner air-conditioning, light compensating lamp, the dehumidifier of being equipped with, by automatic controlling system, temperature 20-31 ℃, humidity 35%-55%.
8. the little phytoseiid mite method of industrialized propagation Chile according to claim 1, it is characterized in that: the Chile's little phytoseiid mite isolation breeding described in step D comprises: when the amount of every basin bean seedlings Tetranychus urticae is 800-1000, move the Chilean little phytoseiid mite chamber of breeding inoculation Predatory Mites to, select the plant leaf that contains the little phytoseiid mite of a large amount of Chile, with scissors, cut gently, be placed in pallet as inoculation carrier, then with tweezers gently gripping blade be placed directly on bean seedlings and inoculate, the little phytoseiid mite of Chile can be climbed to very soon and on bean seedlings, take food Tetranychus urticae, inoculative proportion is that 1:40-50 is advisable.
9. the little phytoseiid mite method of industrialized propagation Chile according to claim 8, it is characterized in that: in the little phytoseiid mite of Chile multiplication plot (6) for preventing that Chilean little phytoseiid mite from running away, breeding of the little phytoseiid mite of Chile adopts movable seedbed (2) to place, in rectangle tank (3) central authorities, " one " word is arranged, apart from tank dual-side edge, be 35cm, water logging in anti-water stopping trough there is not plant, and potted plant heelpiece goes out the water surface; When 40,000 of Chilean little phytoseiid mites of every day breeding are above, can meet 10-30 mu facilities vegetable tetranychid biological control demand.
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Cited By (7)
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| WO2016062979A1 (en) * | 2014-10-23 | 2016-04-28 | Entomo Farm | System and method for the mass rearing of insects |
| CN106234310A (en) * | 2016-08-01 | 2016-12-21 | 河北省农林科学院旱作农业研究所 | A kind of method of efficient breeding Phyloseiulus nersimilis |
| CN107410217A (en) * | 2017-05-16 | 2017-12-01 | 中国热带农业科学院环境与植物保护研究所 | The breeding apparatus and method of cassava monodactyle mite experimental population |
| CN109699593A (en) * | 2019-03-14 | 2019-05-03 | 山西省农业科学院植物保护研究所 | The breeding apparatus and application method of one planting fruit-trees harmful mite |
| CN111226652A (en) * | 2020-04-10 | 2020-06-05 | 山东省植物保护总站 | Greenhouse of supporting formula factory production of natural enemy insect |
| CN112913789A (en) * | 2021-04-02 | 2021-06-08 | 贵州大学 | Predatory mite feeding method taking cattail pollen as feed |
| CN115281158A (en) * | 2022-08-15 | 2022-11-04 | 绵阳市农业科学研究院 | Method for efficient large-scale propagation of phytoseiulus persimilis |
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|---|---|---|---|---|
| WO2016062979A1 (en) * | 2014-10-23 | 2016-04-28 | Entomo Farm | System and method for the mass rearing of insects |
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| CN106234310B (en) * | 2016-08-01 | 2019-04-09 | 河北省农林科学院旱作农业研究所 | A kind of method of efficient breeding Phyloseiulus nersimilis |
| CN107410217A (en) * | 2017-05-16 | 2017-12-01 | 中国热带农业科学院环境与植物保护研究所 | The breeding apparatus and method of cassava monodactyle mite experimental population |
| CN109699593A (en) * | 2019-03-14 | 2019-05-03 | 山西省农业科学院植物保护研究所 | The breeding apparatus and application method of one planting fruit-trees harmful mite |
| CN109699593B (en) * | 2019-03-14 | 2024-02-13 | 山西省农业科学院植物保护研究所 | Fruit tree mite feeding device and use method |
| CN111226652A (en) * | 2020-04-10 | 2020-06-05 | 山东省植物保护总站 | Greenhouse of supporting formula factory production of natural enemy insect |
| CN111226652B (en) * | 2020-04-10 | 2024-05-28 | 山东省植物保护总站 | Greenhouse produced by natural enemy insects in matched factory |
| CN112913789A (en) * | 2021-04-02 | 2021-06-08 | 贵州大学 | Predatory mite feeding method taking cattail pollen as feed |
| CN115281158A (en) * | 2022-08-15 | 2022-11-04 | 绵阳市农业科学研究院 | Method for efficient large-scale propagation of phytoseiulus persimilis |
| CN115281158B (en) * | 2022-08-15 | 2023-09-15 | 绵阳市农业科学研究院 | Method for large-scale propagation of phytoseiid chile |
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