CN113854140B - Modularized porphyra yezoensis seedling three-dimensional culture method - Google Patents
Modularized porphyra yezoensis seedling three-dimensional culture method Download PDFInfo
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- CN113854140B CN113854140B CN202111303889.7A CN202111303889A CN113854140B CN 113854140 B CN113854140 B CN 113854140B CN 202111303889 A CN202111303889 A CN 202111303889A CN 113854140 B CN113854140 B CN 113854140B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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Abstract
The invention discloses a modularized porphyra yezoensis seedling three-dimensional culture method, which comprises the following steps: assembling the modular shell piece; (2) seedling collection of the modularized conch pieces; (3) seedling raising management of the modular conch pieces; and (4) a seedling collection method of the modularized conch shell slices. The invention adopts the modularized shell pieces to replace the traditional hanging culture Bei Kechuan, facilitates the cleaning of shells, saves labor cost, is more uniform in light receiving compared with the traditional mode, is beneficial to improving the seedling collection efficiency, is suitable for being used in the traditional seedling culture pond of the porphyra yezoensis, avoids the transformation of the seedling culture pond, can be upgraded into three-dimensional multilayer culture, and further saves land cost.
Description
Technical Field
The invention relates to a porphyra yezoensis seedling culture method.
Background
The laver is an important economic seaweed in China, has wide industrial range, and drives the coastal social and economic development of China. The laver seedling industry is a main weak link in the laver industry, natural shells are always adopted as seedling culture substrates, and the culture technical method established by the method is greatly influenced by environmental factors. In recent years, the laver cultivation area is rapidly increased, the seedling cultivation area is synchronously increased, the labor cost and the land cost are high, the seedling cultivation technology cannot be completed, the seedling curtain cultivation efficiency is low and extremely unstable, and the method becomes a potential risk for the sustainable development of the industrial health.
At present, seedling culture substrates of laver in various countries are all shells, and the shell mainly comprises clam shells, scallop shells, oyster shells and pearl clam shells. The growth form and the development mode of laver filament in shells are greatly different from those of free filament, and synchronous maturation and synchronous diffusion in the state of the free filament are difficult to achieve, which is also the main reason that shell substrates are still adopted in the current production instead of a suspension culture mode.
The seedling is fixed in maturation time due to the growth characteristics of the shell base and the limitation of supporting facilities, and the requirement of sea area cultivation is more and more not met; the unevenness of the surface of the shell limits batch processing and management, the whole cultivation period needs to be manually washed for many times, the labor intensity is high, the labor cost is high, and the labor is increasingly in short supply; the shell substrate culture adopts plane and hanging culture, and occupies larger land resources. And most of seedling enterprises in China have poor stability due to lagging facility conditions and low technical level, and the seedling curtain breeding rate is low and the risk is high.
The conventional porphyra yezoensis seedling culture mode is a flat culture mode, shells are flatly paved in a seedling culture pond in a scaly mode, the shell is required to be cleaned at regular intervals in the culture mode, the labor cost for cleaning and paving is high, the utilization rate of the seedling culture pond is lower than that of a hanging culture mode, and the shell turning is easily caused to influence the seedling collection efficiency in the seedling collection stage. In the traditional suspension culture, a shell string mode is adopted, so that the seedling collection efficiency is reduced due to the fact that shells are easy to fold, the unit utilization amount is not increased, the water depth needs to be increased, and most of porphyra yezoensis seedling culture ponds are shallow and are not suitable for suspension culture. The method adopts modularized conch slices on the basis of traditional hanging culture, can be used in a traditional Porphyra yezoensis seedling culture pond, and has higher utilization rate than a flat culture mode because laver seedlings can grow on both sides.
Disclosure of Invention
The invention aims to provide a modularized porphyra yezoensis seedling three-dimensional culture method which is convenient for cleaning shells and beneficial to improving the seedling collection efficiency.
The technical solution of the invention is as follows:
a modularized porphyra yezoensis seedling three-dimensional culture method is characterized in that: comprises the following steps:
(1) Assembly of modular conch pieces
The modularized shell slice adopts a pore plate to inlay the shell slice, the shell slice is manually clamped into the pore plate to form a flat shell slice, irregular shell slices with poor firmness are eliminated, the shell slices are picked out after seedling picking, and the shell slices are cleaned and reused;
(2) Seedling collection of modularized conch pieces
Cleaning the front and back surfaces of a shell slice, using the cleaned front and back surfaces of the shell slice for shell seedling collection, hanging a modularized shell slice in a seedling culture pond by using a cross rod, wherein the density of filament seedling collection is 300 strains/cm & lt 2 & gt, the water temperature of the seedling collection is 15-20 ℃, shading is carried out by using black plastic cloth two days before seedling collection, and then, the filament seedling collection is cultured to be mature under the conditions of illumination intensity of 1500-2500 Lux and temperature of 15-27 ℃;
(3) Seedling management of modular shellfish slices
The shell tablet is cleaned once every 15-20 days in a cleaning mode: arranging a transverse plate frame with the width of more than 20 cm on the seedling raising pool, turning up the shell pieces, putting the shell pieces on the transverse plate, scrubbing one surface of each shell piece by using rags or sponges and the like, and cleaning the other surface of each shell piece on the opposite side after all the shell pieces are cleaned; then, the dirt on the shell pieces is further removed in a spraying mode, and the seawater in a sedimentation tank is added for continuous culture after the wastewater is discharged; the direction of the shell piece is adjusted after each cleaning, so that the uniform light receiving is ensured;
(4) Seedling picking method for modularized conch slices
Adjusting the illumination to 8 late-month ten days to start to form sporangial branches, controlling the water temperature to be not lower than 25 ℃, adopting flowing water and increasing and decreasing the cleaning times to regulate and control the shell pieces to be mature, adopting a moderate cooling mode to promote the shell spores to be diffused 3 days before exploitation, increasing the illumination intensity of a seedling raising chamber after exploitation, and putting a submersible pump into a seedling raising pool to flush water to stimulate the diffusion of the shell spores; checking the scattering amount of the small shell pieces of each seedling raising pool every morning to calculate the total scattering amount of the seedling raising pools, determining the quantity of laver net curtains for picking the seedlings, adjusting the total scattering amount by increasing the row number of the small shell pieces, communicating spore water of the seedling raising pools with empty pools, and picking the seedlings in the empty pools in a soaking, flushing or water wheel type seedling picking mode.
The pore plate comprises a rectangular pore plate consisting of square small grids, and 2-4 hangers for hanging are arranged at the upper end of the rectangular pore plate; the small grid holes of each square small grid are grid holes for embedding and fixing the conch slices, and clamping protrusions for fixing the conch slices are arranged on the front and back surfaces of the wall of each small grid hole of each square small grid; two clamping protrusions are arranged on each of the four side walls on the reverse side of each small grid hole; and clamping bulges are arranged on two opposite side walls of the four side walls on the front surface of each small grid hole.
The invention adopts the modularized shell slices to replace the traditional hanging culture Bei Kechuan, facilitates the shell cleaning, saves the labor cost, is more uniform in light receiving compared with the traditional mode, is beneficial to improving the seedling collection efficiency, is suitable for being used in the traditional seedling culture pond of the porphyra yezoensis, avoids the reformation of the seedling culture pond, can be upgraded into the three-dimensional multilayer culture, and further saves the land cost.
Drawings
The invention is further illustrated by the following figures and examples.
FIG. 1 is a schematic view of a nursery pond according to the present invention.
FIG. 2 is a schematic view of the construction of the orifice plate.
Fig. 3 is a schematic structural view of a square small square grid.
Detailed Description
A modularized porphyra yezoensis seedling three-dimensional culture method comprises the following steps:
(1) Assembly of modular shell pieces
The modularized shell slice 1 is embedded with shell slices by adopting a pore plate, the shell slices are manually clamped into the pore plate to form a flat shell slice, irregular shell slices with poor firmness are eliminated, the shell slices are picked out after seedling picking, and the shell slices are cleaned for reuse;
(2) Seedling collection of modularized conch pieces
Cleaning the front and back surfaces of a shell slice for shell seedling collection, hanging a modularized shell slice in a seedling pool 2 by a cross rod 3 through a suspension loop 6, wherein the density of filament seedling collection is 300 plants/cm < 2 >, the temperature of seedling collection water is 15-20 ℃, black plastic cloth is adopted for shading two days before seedling collection, and then the shell slice is cultured to be mature under the conditions that the illumination intensity is 1500Lux-2500Lux and the temperature is 15-27 ℃;
(3) Seedling management of modular shellfish
The shell tablet is cleaned once every 15-20 days in a cleaning mode: arranging a transverse plate frame with the width of more than 20 cm on the seedling raising pool, turning up the shell pieces, putting the shell pieces on the transverse plate, scrubbing one surface of each shell piece by using rags or sponges and the like, and cleaning the other surface of each shell piece on the opposite side after all the shell pieces are cleaned; then, the dirt on the shell pieces is further removed in a spraying mode, and the seawater in a sedimentation tank is added for continuous culture after the wastewater is discharged; the direction of the shell piece is adjusted after each cleaning, so that the uniform light receiving is ensured;
(4) Seedling picking method for modularized conch pieces
Adjusting the illumination to 8 late-month ten days to start to form sporangial branches, controlling the water temperature to be not lower than 25 ℃, adopting flowing water and increasing and decreasing the cleaning times to regulate and control the shell pieces to be mature, adopting a moderate cooling mode to promote the shell spores to be diffused 3 days before exploitation, increasing the illumination intensity of a seedling raising chamber after exploitation, and putting a submersible pump into a seedling raising pool to flush water to stimulate the diffusion of the shell spores; checking the scattering amount of the small shell pieces of each seedling raising pool every morning to calculate the total scattering amount of the seedling raising pools, determining the quantity of laver net curtains for picking the seedlings, adjusting the total scattering amount by increasing the row number of the small shell pieces, communicating spore water of the seedling raising pools with empty pools, and picking the seedlings in the empty pools in a soaking, flushing or water wheel type seedling picking mode.
The pore plate comprises a rectangular pore plate consisting of square small grids, and 2-4 hangers 6 for hanging are arranged at the upper end of the rectangular pore plate; the small grid holes of each square small grid are grid holes for embedding and fixing the conch slices, and clamping protrusions for fixing the conch slices are arranged on the front and back surfaces of the wall of each small grid hole of each square small grid; two clamping protrusions 4 are arranged on each of the four walls on the reverse side of each small grid hole; and clamping protrusions 5 are arranged on two opposite side walls of the four side walls on the front surface of each small grid hole.
The specification of the small grid holes is as follows: length 25mm, width 25mm, thickness 4 mm; the specifications of the clamping process are as follows: 1-3mm wide, 1 mm high, 1 mm thick.
The rectangular orifice plate of the invention is made of the following materials: PP, PE, ABS, PVC, and the like.
Claims (1)
1. A modularized porphyra yezoensis seedling three-dimensional culture method is characterized in that: comprises the following steps:
(1) Assembly of modular conch pieces
The modularized shell slice adopts a pore plate to inlay a small shell slice, the small shell slices are manually clamped into the pore plate to form a flat modularized shell slice, the small shell slices with irregularity and poor firmness are eliminated, the small shell slices are removed after seedling collection is finished, and the small shell slices are cleaned and reused;
(2) Seedling collection of modularized conch pieces
Cleaning the front and back surfaces of a modularized shell slice, using the cleaned front and back surfaces of the modularized shell slice for shell seedling collection, hanging the modularized shell slice in a seedling culture pond by using a cross rod, wherein the density of filament seedling collection is 300 plants/cm & lt 2 & gt, the water temperature of the seedling collection is 15-20 ℃, shading is performed by using black plastic cloth two days before seedling collection, and then the filament seedling collection is cultured to be mature under the conditions of illumination intensity of 1500-2500 Lux and temperature of 15-27 ℃;
(3) Seedling management of modular shellfish
The modularized shell slice is cleaned once every 15-20 days in a cleaning mode: a transverse plate frame with the width of more than 20 cm is arranged on the seedling raising pool, the modular shell pieces are turned up and placed on the transverse plate, cleaning cloth or sponge is adopted to scrub one surface of each modular shell piece, and the other surface is cleaned on the opposite side after all the shell pieces are cleaned; then, further removing dirt on the modularized shell sheets in a spraying mode, and adding seawater in a sedimentation tank for continuous culture after discharging wastewater; the direction of the modularized shell piece is adjusted after each cleaning, so that the uniform light receiving is ensured;
(4) Seedling picking method for modularized conch slices
Adjusting the illumination to 8 late-month ten days to start forming sporangial branches, controlling the water temperature to be not lower than 25 ℃, adopting flowing water and increasing and decreasing the cleaning times to regulate the modular conchospore pieces to be mature, adopting a moderate cooling mode to promote the diffusion of conchospores 3 days before mining, increasing the illumination intensity of a seedling raising chamber after mining, and putting a submersible pump into a seedling raising pool to flush water to stimulate the diffusion of the conchospores; checking the scattering amount of small shells of each seedling raising pool at regular time every morning to calculate the total scattering amount of the seedling raising pools, determining the number of laver net curtains for picking the seedlings, adjusting the total scattering amount by increasing the row number of the modularized small shells, communicating spore water of the seedling raising pools with an empty pool, and picking the seedlings in the empty pool in a soaking, flushing or water-wheel type seedling picking mode;
the pore plate comprises a rectangular pore plate consisting of square small grids, and 2-4 hangers for hanging are arranged at the upper end of the rectangular pore plate; the small square grid hole of each square small grid is a grid hole for embedding and fixing a shell small piece, and clamping protrusions for fixing the shell small piece are arranged on the front side and the back side of the wall of the small square grid hole of each square small grid; two clamping protrusions are arranged on each of the four side walls on the reverse side of each small grid hole; among the four walls on the front surface of each small grid hole, two opposite walls are provided with clamping protrusions.
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Citations (3)
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| JP2005102615A (en) * | 2003-09-30 | 2005-04-21 | Daiichi Seimou Co Ltd | Base material for culture for culturing marine alga |
| CN104170593A (en) * | 2014-08-06 | 2014-12-03 | 江苏省海洋水产研究所 | Indoor conchospore seedling picking method for porphyra haitanensis shell conchocelis |
| CN104365468A (en) * | 2014-11-12 | 2015-02-25 | 江苏省海洋水产研究所 | Stereoscopic culturing system and method of porphyra yezoensis shell protonema |
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| JPH07147864A (en) * | 1993-11-30 | 1995-06-13 | Nippon Light Metal Co Ltd | Culture reef and method for its utilization |
| CN104429913B (en) * | 2014-12-23 | 2016-09-07 | 淮海工学院 | Bonding combined type laver seedling culture medium |
| CN107750937A (en) * | 2017-10-24 | 2018-03-06 | 山东省海洋生物研究院 | A kind of attachment base suitable for Porphyra yezoensis conchocelis breeding prepares and its application method |
| CN107616088A (en) * | 2017-10-26 | 2018-01-23 | 中国水产科学研究院黄海水产研究所 | The vertical device for raising seedlings of one main laver shell conchocelis and its application method |
| KR101935525B1 (en) * | 2018-06-07 | 2019-01-04 | 이승환 | Conchospore plant culture-net |
| CN211319129U (en) * | 2019-10-30 | 2020-08-21 | 郑州工业应用技术学院 | Fixed bolster convenient to installation of computer hard disk |
| CN111316908B (en) * | 2020-04-03 | 2022-02-08 | 江苏省海洋资源开发研究院(连云港) | Laver seedling shell net curtain and arranging and sewing device thereof |
| CN112219713B (en) * | 2020-10-28 | 2022-05-13 | 连云港东浦建筑工业化发展有限公司 | Assembled laver seedling raising substrate and using method thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005102615A (en) * | 2003-09-30 | 2005-04-21 | Daiichi Seimou Co Ltd | Base material for culture for culturing marine alga |
| CN104170593A (en) * | 2014-08-06 | 2014-12-03 | 江苏省海洋水产研究所 | Indoor conchospore seedling picking method for porphyra haitanensis shell conchocelis |
| CN104365468A (en) * | 2014-11-12 | 2015-02-25 | 江苏省海洋水产研究所 | Stereoscopic culturing system and method of porphyra yezoensis shell protonema |
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