CN104170557B - A kind of method promoting cherry seed rapidly and efficiently Germination And Seedling - Google Patents
A kind of method promoting cherry seed rapidly and efficiently Germination And Seedling Download PDFInfo
- Publication number
- CN104170557B CN104170557B CN201410360527.5A CN201410360527A CN104170557B CN 104170557 B CN104170557 B CN 104170557B CN 201410360527 A CN201410360527 A CN 201410360527A CN 104170557 B CN104170557 B CN 104170557B
- Authority
- CN
- China
- Prior art keywords
- seed
- seedling
- days
- pit
- germination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
Abstract
Embodiments provide a kind of method promoting cherry seed rapidly and efficiently Germination And Seedling, relate to seed treatment field, it is possible to improve the germination percentage of cherry seed, shorten Fructus Pruni pseudocerasi breeding or seedling raise period.Described method includes: after Fructus Pruni pseudocerasi full maturity, and collecting fruit removes surface sarcocarp, dries pit and obtains clean pit, be placed in 4 DEG C of refrigerators standby after washing by normal-temperature water；Remove the shell of clean pit, or remove after shell peels off the seed coat being coated plumular axis again, uniformly it is placed in the container being covered with two-layer wet towel, the wet towel of the same humidity of lid two-layer herein above again, it is placed under the environment of 0 DEG C-4 DEG C and induces 5-7 days, taking out seed and be seeded on kind of bed by the seeding row spacing of 2 × 3cm, cultivating 3-6 days at normal temperatures, sprouting is emerged；When seedling true leaf expansion height of seedling reaches 3-5cm, spray the potassium dihydrogen phosphate of a time 0.2% every 7 days blade faces, until seedling is colonizated in field after growing to more than 15cm.
Description
Technical field
The present invention relates to seed treatment field, particularly relate to a kind of method promoting cherry seed rapidly and efficiently Germination And Seedling.
Background technology
Cherry is listing in spring fruit the earliest, is regulating fresh fruit market supply in dull season, and meeting people's living needs aspect has special effect.Its fruit colour is bright-coloured, sparkling and crystal-clear beauty, nutritious, and outward appearance and interior quality are all good, is described as " in fruit treasure ", have the good reputation of " spring fruit first ".
A longer rest period is had after kernel approaches seed collection, only could germination and growth after seed breaking dormancy.Cherry is short due to fruit development period, about 40 days are only needed from blooming to fruit maturation, easily occur that embryo is not grown fully and caused percentage of seedgermination relatively low, and seed dormancy is chronic, these biological characteristicses for hybrid Seedling sprout ahead of time and the cultivation of excellent nursery stock is always up a serious bottleneck, and seriously limit people to a certain extent to the research of these excellent species and utilization.For this, the dormancy mechanism of Fructus Pruni pseudocerasi seed has been done more exploratory development by forefathers, jointly think that Fructus Pruni pseudocerasi seed only could breaking dormancy after the cold stratification of long period, Germination And Seedling, specifically, cherry at least needs 100-180d, the gean of nearly edge, Fructus Pruni Tomentosae and sour cherry etc. then take longer for, be respectively necessary for 150d, 180-240d and 200-300d, even and if carry out embryo culture, it is necessary to the K cryogenic treatment through at least 40d could produce normal test tube Seedling, and cost and technology require higher.
Traditional cherry seed dormancy of breaking promotes that the method for rudiment mainly has conventional Stratificated treatment and uses gibberellin treatment.Wherein conventional layer area method is that the cherry under just adopting is removed sarcocarp, carries out wet Tibetan immediately in the wet husky ratio of 3 parts of 1 part of seed after cleaning.Taking out seed before frozen ground, lamination is located in overhead about 20cm, and Yu Yichun sows in accelerating germination.The method be most widely used general be also most efficient method.But seed treatment program is complicated, time-consuming take a lot of work, and wet hide during on the occasion of hot and humid summer, the easy moldy metamorphism of seed, also longer for the cycle cross-breeding.And gibberellin treatment method is the method adopting certain density gibberellin treatment seed to promote to sprout with breaking dormancy for different Fructus Pruni pseudocerasi types, but the method needs to explore different concentration for different Fructus Pruni pseudocerasi types, and rudiment and planting percent are relatively low, cost is also higher.
Therefore, although forefathers have been achieved for certain progress in breaking Fructus Pruni pseudocerasi seed dormancy, but these technology are all relatively time-consuming, effort, it is difficult to produce a desired effect, it is often more important that germination percentage is relatively low.
Summary of the invention
The invention provides and a kind of promote that cherry seed is quick, economical, the method for efficient Germination And Seedling, improving its germination percentage simultaneously, substantially reduce breeding (nursery) time.
The technical scheme that a kind of method promoting cherry seed rapidly and efficiently Germination And Seedling is concrete is as follows:
A., after cherry full maturity, gather cherry fruit and remove its surface sarcocarp acquisition pit, dry pit surface moisture after washing by normal-temperature water and obtain clean pit, be placed in 4 DEG C of refrigerators standby；
B. clean pit shell standby in step A is cracked, remove shell, or peel off, after removal shell, the seed coat being coated plumular axis again；Seed after shelling or removing the peel uniformly is placed in the container being covered with two-layer wet towel, then the wet towel of the same humidity of lid two-layer herein above, it is placed under the environment of 0 DEG C-4 DEG C and induces 5-7 days, sprout to most of seed base-root.
C. taking out the seed after bringing out and be seeded in by the seeding row spacing of 2 × 3cm on the kind bed completed, cultivating 3-6 days under normal temperature condition, sprouting is emerged；Described room temperature is 23-28 DEG C；
D., when seedling true leaf expansion height of seedling reaches 3-5cm, the potassium dihydrogen phosphate of a time 0.2% is sprayed every 7 days blade faces, until seedling is colonizated in field after growing to more than 15cm.
Optionally, the clean pit collected in step A for step B, was then placed on 4 DEG C of refrigerators standby in 2-3 days；If more than 3 days for step B, then the pit collected and the damp sand of water content 30%-40% or Vermiculitum are mixed in the ratio of 1: 3 to be placed on 4 DEG C of refrigerators standby.
Optionally, seed can not be injured when step B removes pit shell；Only need to peel off the seed coat of one end that plumular axis is sprouted when peelling off seed coat, preventing and treating injures embryo.
Optionally, wet towel described in step B is before use through autoclaving 25min at the temperature of 121 DEG C, the water content of described wet towel is 35%-45% (being preferably 40%), should check towel humidity every day, and change towel in time during step B.
Optionally, the kind bed described in step C is laid by Vermiculitum and is formed, if the Vermiculitum planted on bed is new Vermiculitum, without sterilization；Otherwise Vermiculitum carries out disinfection with 800 times of liquid of carbendazim and 1000 times of liquid of thiophanate methyl before use, and on described kind bed, Vermiculitum thickness is about 10cm；Should strictly control the humidity of kind of bed during this period so that it is humidity is maintained at the water content of 40%-50%.
Optionally, the standard of the Seed germination and emergence described in step C is that hypocotyl growth length is more than 3mm.
The method promoting cherry seed rapidly and efficiently Germination And Seedling that technique scheme provides, according to the method for the present invention, seed is carried out skin breaking, after 0 DEG C of-4 DEG C of low temperature induction 7 days, germination percentage after cultivating 3 days on room temperature kind bed is up to 75%, and when 9 days, major part seedling true leaf launches.Only need 30-40 days to get final product seedling from seed collection, height of seedling reaches more than 15cm, and planting percent is up to more than 80%, and traditional method needs the time of 10 months from seed collection to seedling, and planting percent is relatively low.Therefore the method adopting the present invention can be greatly saved Fructus Pruni pseudocerasi nursery or breeding time.And method provided by the invention is without special instruments and equipment and other plant growth regulators, simple to operate, cost is low, and germination percentage and planting percent are all higher.
It addition, according to method provided by the invention, can directly process after Fructus Pruni pseudocerasi seed collection and carry out Germination And Seedling, it is not necessary to the sand through the long period hides Stratificated treatment, thus less Some seeds rotting and rotten in level processing procedure, improve seed utilization rate.
Accompanying drawing explanation
Fig. 1 is seed low temperature induction situation diagram of shelling；
Fig. 2 is the sprouting situation diagram that cultivation cherry seed low temperature induction is sowed after 7 days 3 days；
Fig. 3 is the sprouting situation diagram that wild Chinese Fructus Pruni pseudocerasi seed low temperature induction is sowed after 7 days 6 days；
Fig. 4 is the sprouting situation diagram that wild Chinese Fructus Pruni pseudocerasi seed low temperature induction is sowed after 7 days 9 days；
Fig. 5 is situation of the emerging diagram that hybridization cherry seed low temperature induction is sowed after 7 days 18 days.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art obtain under not making creative work premise, broadly fall into the scope of protection of the invention.
Embodiments providing a kind of method promoting Fructus Pruni pseudocerasi seed rapidly and efficiently Germination And Seedling, its detailed step is described below is:
A. after Fructus Pruni pseudocerasi full maturity, gather cultivation cherry (Yaan red cherry) seed, wild Chinese Fructus Pruni pseudocerasi (Yaan Zhou Gongshan) seed and each 1800 of cherry hybrid seed (No. 2 × Luding of small stream, wild osmanthus red cherry) respectively, remove surface sarcocarp, dry pit surface moisture after washing by normal-temperature water, be placed in 4 DEG C of refrigerators standby.
B. in 2-3 days, ready clean pit is divided into 3 equal portions, is 3 process, 200 every part, point 3 repetitions.Wherein first part is comparison, not decapsidate, pit shell is cracked by second part with hammer or pliers, note being sure not to injure seed, peel off, after 3rd part of removal shell (endocarp), the seed coat being coated plumular axis again, then all seeds are uniformly placed on and are covered with two-layer in the iron pan of autoclaved wet towel, the towel of the same humidity of lid two-layer herein above again, towel humid control is in the water content of 35%-45% (being preferably 40%), it is subsequently placed under the environment of 0 DEG C-4 DEG C and induces 7 days, the humidity of towel should be strictly controlled during noting this, until major part seed base-root is sprouted.
C. taking out Germinating Seeds and be seeded on the kind bed that prior sterilized Vermiculitum is completed the nutritive cube of 16 × 14 (this with) by 2 × 3cm seeding row spacing, Vermiculitum thickness is 8-12cm, it is preferred that for 10cm.Under normal temperature condition, (23-28 DEG C) is cultivated 9 days, adds up the germinative number of every day, and calculates germination percentage.Should strictly control the humidity of kind of bed during this period so that it is humidity is maintained at the water content of 40%-50%.
D., when seedling true leaf expansion height of seedling reaches 3-5cm, spray the potassium dihydrogen phosphate of a time 0.2% every 7 days blade faces, until seedling is colonizated in field after growing to more than 15cm, add up planting percent.
Key data assembles opinion:
Table 1 gives and adopts in step B the sprouting situation of different cherry seeds after different disposal method.Visible collection seed after cherry fruit fully maturation, through skin breaking and broken shell and go induction under the environment that the seed that seed coat processes is placed in 0 DEG C-4 DEG C to be seeded on the kind bed completed with sterilized Vermiculitum in advance after 7 days, under normal temperature condition (23-28 DEG C) cultivate 9 days after germination percentage and final planting percent all pole be significantly higher than the seed of not skin breaking.And the dissimilar interseminal germination rate of cherry and planting percent difference are little, specifically as shown in table 1.
With regard to sprouting time, the seed of not broken shell is not also sprouted after low temperature induction, and the seed of skin breaking is the highest at the germination percentage of after planting 4 days through low temperature induction 7 days, and the highest at the germination percentage of after planting 3 days of seed coat is gone in broken shell.And germinate and focus mostly on 2-5 days after planting, specifically as shown in table 2.
Germination percentage under different disposal of table 1, different cherry seed and planting percent statistics
Table 2, different cherry seed germination process under different disposal
For the seed without skin breaking after 0 DEG C of-4 DEG C of low temperature induction 7 days, the germination percentage after cultivating 9 days on room temperature kind bed is 0；And according to the method for the present invention, seed is carried out skin breaking, after 0 DEG C of-4 DEG C of low temperature induction 7 days, the germination percentage after cultivating 3 days on room temperature kind bed is up to 75%, and when 9 days, major part seedling true leaf launches.Only need 30-40 days to get final product seedling from seed collection, height of seedling reaches more than 15cm, and planting percent is up to more than 80%.And traditional method needs the time of 10 months from seed collection to seedling in prior art, and planting percent is relatively low.Therefore the method adopting the present invention can improve planting percent, and is greatly saved Fructus Pruni pseudocerasi nursery or breeding time.
The above; being only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, any those familiar with the art is in the technical scope that the invention discloses; the change that can readily occur in or replacement, all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should described be as the criterion with scope of the claims.
Claims (6)
1. the method promoting cherry seed rapidly and efficiently Germination And Seedling, it is characterised in that including:
A., after cherry full maturity, gather cherry fruit and remove its surface sarcocarp acquisition pit, dry pit surface moisture after washing by normal-temperature water and obtain clean pit, be placed in 4 DEG C of refrigerators standby；
B. clean pit shell standby in step A is cracked, remove shell, or peel off, after removal shell, the seed coat being coated plumular axis again；Seed after shelling or removing the peel uniformly is placed in the container being covered with two-layer wet towel, then the wet towel of the same humidity of lid two-layer herein above, it is placed under the environment of 0 DEG C-4 DEG C and induces 5-7 days；
C. taking out the seed after bringing out and be seeded in by the seeding row spacing of 2 × 3cm on the kind bed completed, cultivating 3-6 days under normal temperature condition, sprouting is emerged；Described room temperature is 23-28 DEG C；
D., when seedling true leaf expansion height of seedling reaches 3-5cm, the potassium dihydrogen phosphate of a time 0.2% is sprayed every 7 days blade faces, until seedling is colonizated in field after growing to more than 15cm.
2. method according to claim 1, it is characterised in that the clean pit collected in step A for step B, was then placed on 4 DEG C of refrigerators standby in 2-3 days；If more than 3 days for step B, then the pit collected and the damp sand of water content 30%-40% or Vermiculitum are mixed in the ratio of 1: 3 to be placed on 4 DEG C of refrigerators standby.
3. method according to claim 1, it is characterised in that seed can not be injured when removing pit shell in step B；The seed coat of one end that plumular axis is sprouted only need to be peelled off, it is prevented that injure embryo when peelling off seed coat.
4. method according to claim 1, it is characterized in that, the wet towel described in step B is before use through autoclaving 25min at the temperature of 121 DEG C, and the water content of described wet towel is 35%-45%, towel humidity should be checked during step B every day, and change towel in time.
5. method according to claim 1, it is characterised in that the kind bed described in step C is laid by Vermiculitum and formed, if the Vermiculitum planted on bed is new Vermiculitum, without sterilization；Otherwise Vermiculitum carries out disinfection with 800 times of liquid of carbendazim and 1000 times of liquid of thiophanate methyl before use, and on described kind bed, Vermiculitum thickness is 8-12cm；Should strictly control the humidity of kind of bed during this period so that it is humidity is maintained at the water content of 40%-50%.
6. method according to claim 1, it is characterised in that the standard of the Seed germination and emergence described in step C is that hypocotyl growth length is more than 3mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410360527.5A CN104170557B (en) | 2014-07-24 | 2014-07-24 | A kind of method promoting cherry seed rapidly and efficiently Germination And Seedling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410360527.5A CN104170557B (en) | 2014-07-24 | 2014-07-24 | A kind of method promoting cherry seed rapidly and efficiently Germination And Seedling |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104170557A CN104170557A (en) | 2014-12-03 |
| CN104170557B true CN104170557B (en) | 2016-06-29 |
Family
ID=51952134
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410360527.5A Expired - Fee Related CN104170557B (en) | 2014-07-24 | 2014-07-24 | A kind of method promoting cherry seed rapidly and efficiently Germination And Seedling |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104170557B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105027962B (en) * | 2015-09-08 | 2017-05-03 | 洱源县印象林果庄园有限公司 | Root and crown controlling cultivation method for large cherry trees |
| CN105284234B (en) * | 2015-09-26 | 2017-10-03 | 大连市农业科学研究院 | A kind of method for improving sweet cherry percentage of seedgermination |
| CN107079634A (en) * | 2017-04-05 | 2017-08-22 | 周兴虎 | A kind of flowering cherry seed poison-removing method |
| CN107258141B (en) * | 2017-07-05 | 2021-02-02 | 北京林业大学 | Plum blossom seed germination method |
| CN107820777B (en) * | 2017-11-14 | 2020-11-06 | 湖北省农业科学院果树茶叶研究所 | Method for improving germination rate of peach seeds |
| CN108901575A (en) * | 2018-09-05 | 2018-11-30 | 四川农业大学 | A kind of three step cultivating methods improving cherry hybrid planting percent and precocity fruiting |
| CN111602550A (en) * | 2020-03-25 | 2020-09-01 | 普洱大华林业科技有限公司 | Artificial seedling raising method using taxus chinensis seeds |
| CN114982560B (en) * | 2022-06-17 | 2024-04-12 | 河北双星种业股份有限公司 | Method for raising seedlings by utilizing sunflower seeds |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080214443A1 (en) * | 2004-04-09 | 2008-09-04 | Baum James A | Compositions and Methods for Control of Insect Infestations in Plants |
| CN103348799A (en) * | 2013-07-19 | 2013-10-16 | 镇江瑞繁农艺有限公司 | Cultivation method for repeated seed collection of verbena hybrida |
| CN103430807A (en) * | 2013-08-16 | 2013-12-11 | 天水市果树研究所 | Method for cultivating cherry seedlings |
-
2014
- 2014-07-24 CN CN201410360527.5A patent/CN104170557B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080214443A1 (en) * | 2004-04-09 | 2008-09-04 | Baum James A | Compositions and Methods for Control of Insect Infestations in Plants |
| CN103348799A (en) * | 2013-07-19 | 2013-10-16 | 镇江瑞繁农艺有限公司 | Cultivation method for repeated seed collection of verbena hybrida |
| CN103430807A (en) * | 2013-08-16 | 2013-12-11 | 天水市果树研究所 | Method for cultivating cherry seedlings |
Non-Patent Citations (4)
| Title |
|---|
| 不同层积方法对核果类种子萌发率的影响;杜纪红 等;《中国南方果树》;20140520;第43卷(第3期);第107-109页 * |
| 樱桃和杏种子发芽育苗试验;辛力 等;《中国果树》;19850501(第02期);第16-18页 * |
| 樱桃种子的休眠与萌发;刘用生 等;《烟台果树》;19950215;第30-31页 * |
| 樱桃种子的发芽初探;徐本美;《种子》;19880425(第02期);第35-37页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104170557A (en) | 2014-12-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104170557B (en) | A kind of method promoting cherry seed rapidly and efficiently Germination And Seedling | |
| CN101711493B (en) | Propagation of lily seedball by directly peeling scale | |
| CN104186190B (en) | Fruit tree hybrid seed sprouting and rooting method | |
| CN103875529A (en) | Blueberry tissue culture propagation and ex-vitro rooting method | |
| CN101116424B (en) | Highly effective lily bulblet inducement culture method | |
| CN104642000B (en) | Manglietia lucida seed germinating and seedling method | |
| CN106172011B (en) | A kind of dwarf banana high temperature light culture tissue culture and rapid propagation method | |
| CN110476764B (en) | Industrial paris polyphylla seedling raising method | |
| CN109526298B (en) | Passion fruit seed treatment method and sowing and breeding method thereof | |
| CN107494230A (en) | A kind of spring method for culturing seedlings of Acer palmatum seed | |
| CN105900564B (en) | A kind of method that rare or endangered species beet seeds is promoted efficiently to sprout | |
| CN107852886A (en) | A kind of method for promoting swordleaf dragon tree seed to sprout | |
| CN108966741B (en) | Method for improving germination rate and accelerating germination of Japanese Rhus succedanea seeds | |
| CN109511534B (en) | Berry seed sowing and breeding method | |
| CN105075860B (en) | A kind of fleabane flower tissue culture method | |
| CN106613833A (en) | Fiddlehead spore seedling culture method | |
| CN104585040A (en) | Method for quickly propagating germchit of giantleaf ardisia rhizome through bud plumular axis | |
| CN104025762B (en) | A kind of method promoting purple striae lip post lettuce tongue seed germination | |
| CN103069948A (en) | Seeding method of broad beans | |
| CN106817951A (en) | A kind of method of pawpaw seed biogas slurry breeding vernalization | |
| CN103843545A (en) | Method for domesticating and cultivating high-quality rootstock seedlings by utilizing wild eggplant seeds | |
| CN105265061A (en) | Method for improving germination rate of Lithocarpus hancei seeds | |
| CN105941161A (en) | Tissue culture method of phalaenopsis | |
| CN105325096A (en) | Method for promoting germination of lithocarpus amygdalifolius seeds | |
| CN105519278A (en) | Application of vegetable amino acid fertilizer in seed germination of Magnolia zenii |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160629 Termination date: 20170724 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |