CN107494230A - A kind of spring method for culturing seedlings of Acer palmatum seed - Google Patents

A kind of spring method for culturing seedlings of Acer palmatum seed Download PDF

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CN107494230A
CN107494230A CN201710874690.7A CN201710874690A CN107494230A CN 107494230 A CN107494230 A CN 107494230A CN 201710874690 A CN201710874690 A CN 201710874690A CN 107494230 A CN107494230 A CN 107494230A
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seed
acer palmatum
days
vernalization
germination
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王光晋
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Anhui Million Orchard Agricultural Science And Technology Development Co Ltd
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Anhui Million Orchard Agricultural Science And Technology Development Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10Animals; Substances produced thereby or obtained therefrom
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B1/00Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02Superphosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Agronomy & Crop Science (AREA)
  • Soil Sciences (AREA)
  • Cultivation Of Plants (AREA)
  • Fertilizers (AREA)

Abstract

The invention mainly relates to planting technology field, discloses a kind of spring method for culturing seedlings of Acer palmatum seed, including:Immersion, fermentation, freezing, vernalization, sowing, management;Method is simple, easy to operate, lamination when avoiding traditional wet sand long, germination time is shorten to 4 ~ 5 days, germination in after planting 7 ~ 9 days is unearthed, and germination percentage reaches 94.7%, hence it is evident that improves the spring breeding efficiency of Acer palmatum;Dry seed is placed in sodium hydroxide solution before sowing and soaked, accelerates to destroy seed coat structure, the nutrition and moisture for promoting the external world are immersed, and are suppressed dormancy species activity, are accelerated germination;Seed is placed in streptococcus acidi lactici fermented solution after immersion, by lactic acid bacteria it is long when ferment, regulation seed pH, a large amount of metabolites caused by fermentation are promoted to immerse kind of a skin, break seed dormancy, promote plumule growth, shorten the vernalization of seed and germination unearthed time after planting.

Description

A kind of spring method for culturing seedlings of Acer palmatum seed
Technical field
The invention mainly relates to planting technology field, more particularly to a kind of spring method for culturing seedlings of Acer palmatum seed.
Background technology
Acer palmatum, also known as Japanese maple, tree crown umbrella shape, posture Artline, trade can be made and view and admire tree plant, be preferably " four It is other " green tree species, the breeding stock generally use seeding growing seedlings method of Acer palmatum, it is necessary in wet sand after October seed harvest Lamination to 2 ~ March of next year could be sowed, and also inspect periodically and clean seed during lamination, and process is cumbersome, Er Qiebo It could be unearthed within 25 ~ 30 days after kind, germination percentage is 85% or so, and growing-seedling period is oversize, makes the breeding efficiency of Acer palmatum substantially reduce.
The content of the invention
The defects of in order to make up prior art, it is an object of the invention to provide a kind of spring nursery side of Acer palmatum seed Method.
A kind of spring method for culturing seedlings of Acer palmatum seed, comprises the following steps:
(1)Immersion:Before spring sowing, dry seed is placed in sodium hydroxide solution and soaked 4 ~ 5 hours, accelerate to destroy kind of a leather block Structure, the nutrition and moisture for promoting the external world are immersed, suppress dormancy species activity, accelerate germination, clean, obtain immersion seed;
(2)Fermentation:Immersion seed is placed in streptococcus acidi lactici fermented solution, fermented 25 ~ 30 hours in 41 ~ 43 DEG C, adjusts seed pH, is promoted Enter a large amount of metabolites caused by fermentation and immerse kind of a skin, break seed dormancy, promote plumule growth, shorten the vernalization of seed and broadcast Germination unearthed time after kind, filtering, obtains fermentation seed and zymotic fluid;
(3)Freezing:Fermentation seed is placed in into -34 ~ -32 DEG C to freeze 10 ~ 12 hours, takes out and is warming up to room temperature after 34 ~ 36 DEG C, make The moisture of kind skin attachment is changed into the crystallization water, the tissue voids of increase kind skin, promotes the immersion of external nourishment composition, stimulates plumule to give birth to It is long, strengthen plumule resistance, obtain freezing seed;
(4)Vernalization:Freezing seed is placed in germinating box, the temperature for adjusting germinating box is 24 ~ 26 DEG C, humidity is 82 ~ 84%, quiet Put vernalization 4 ~ 5 days, obtain vernalization seed;
(5)Sowing:Vernalization seed is seeded in seedling medium, upper strata covering 0.6 ~ 1.1cm of plant ash, uniformly sprays step (2)In zymotic fluid, nutrition is balanced, promotes neutron absorption, accelerates plumule growth, improves seed resistance and environmental suitability, Percentage of seedgermination is improved, obtains sowing seed;
(6)Management:Sprinkled water daily, the water content for keeping plant ash is 42 ~ 44%, accelerates germination and is unearthed, sprout is unearthed After cover sunshade net, 3 ~ 5 days after being unearthed, sodium selenite solution that sprinkling mass concentration is 113 ~ 115mg/L, continuous sprinkling 2 times, Interval time is 6 ~ 8 days, makes seedling sturdy, avoids seedling from tanning severely, and accelerates seedling early growth, carries out Routine Management afterwards, obtain chicken feet Maple seedling.
The step(1)Sodium hydroxide solution, mass concentration be 1.1 ~ 1.3%.
The step(2)Streptococcus acidi lactici fermented solution, will lactic acid bacteria access MRS fluid nutrient mediums in, in 40 ~ 42 DEG C ferment 20 ~ 24 hours, obtain streptococcus acidi lactici fermented solution.
Described lactic acid bacteria, it is one in Lactobacillus plantarum, lactobacillus acidophilus, streptococcus thermophilus by re-activation Kind or several any combination.
The step(5)Seedling medium, be made up of the raw material of following parts by weight:Corncob 46 ~ 48, culture medium of edible fungus 37 ~ 39, fallen leaves 33 ~ 35, sawdust 24 ~ 26, biogas residue 21 ~ 23, vinasse 17 ~ 19, plant ash 10 ~ 12, egg shell 7 ~ 9, EM bacterium 5 ~ 7, Vermiculite 17 ~ 19, perlite 13 ~ 15, slag ash 24 ~ 26, inorganic fertilizer 1.6 ~ 1.8, by corncob, culture medium of edible fungus, fallen leaves, Sawdust, biogas residue, vinasse, plant ash and egg shell co-grinding, EM bacterium are accessed, compost fermentation 6 ~ 7 days, add vermiculite, perlite And slag ash, it is well mixed, continues fermentation 3 ~ 4 days, add inorganic fertilizer, is well mixed, obtains seedling medium.
Described inorganic fertilizer, it is made up of the raw material of following parts by weight:Urea 27 ~ 29, calcium superphosphate 21 ~ 23, potassium chloride 22 ~ 24, manganese sulfate 6 ~ 8, borax 4.3 ~ 4.5, zinc sulfate 1.6 ~ 1.8.
It is an advantage of the invention that:The spring method for culturing seedlings of Acer palmatum seed provided by the invention, method is simple, operation side Just, lamination when avoiding traditional wet sand long, germination time is made to shorten to 4 ~ 5 days, germination in after planting 7 ~ 9 days is unearthed, and germination percentage reaches To 94.7%, hence it is evident that improve the spring breeding efficiency of Acer palmatum;Dry seed is placed in sodium hydroxide solution before sowing and soaked Bubble, accelerate to destroy seed coat structure, the nutrition and moisture for promoting the external world are immersed, and are suppressed dormancy species activity, are accelerated germination;Leaching Seed is placed in streptococcus acidi lactici fermented solution after bubble, by lactic acid bacteria it is long when ferment, adjust seed pH, promote big caused by fermentation Measure metabolite and immerse kind of a skin, break seed dormancy, promote plumule growth, the vernalization and germination after planting for shortening seed are unearthed Time;Seed is freezed in short-term after fermentation, kind of the moisture of skin attachment is changed into the crystallization water, increases the tissue voids of kind of skin, Promote external nourishment composition to immerse, stimulate plumule to grow, strengthen plumule resistance;Seed is placed in germinating box after freezing, adjusted Suitable temperature and humidity is saved, accelerates plumule growth, shortens the germination time of seed;Seed is seeded in seedling medium after vernalization In, plant ash is covered, and poured with zymotic fluid, for seedling medium rich in organic and inorganic nutrients composition, nutrition is balanced, promotes Neutron absorption, accelerate plumule growth, improve seed resistance and environmental suitability, improve percentage of seedgermination;After planting keep educating Seedling water content of substrate, accelerate germination and be unearthed, spray sodium selenite solution after being unearthed, and cover sunshade net, make seedling sturdy, Avoid seedling from tanning severely, accelerate seedling early growth.
Embodiment
Illustrate the present invention with specific embodiment below.
Embodiment 1
A kind of spring method for culturing seedlings of Acer palmatum seed, comprises the following steps:
(1)Immersion:Before spring sowing, dry seed is placed in sodium hydroxide solution and soaked 4 hours, mass concentration 1.1%, To accelerate to destroy seed coat structure, the nutrition and moisture for promoting the external world are immersed, and are suppressed dormancy species activity, are accelerated germination, clean, Seed must be soaked;
(2)Fermentation:Immersion seed is placed in streptococcus acidi lactici fermented solution, fermented 25 hours in 41 DEG C, adjusts seed pH, promotes fermentation Caused a large amount of metabolite immerses kind of a skin, breaks seed dormancy, promote plumule growth, shortens the vernalization and after planting of seed Germinate unearthed time, filtering, obtains fermentation seed and zymotic fluid;Described streptococcus acidi lactici fermented solution, lactic acid bacteria is accessed into the training of MRS liquid Support in base, fermented 20 hours in 40 DEG C, obtain streptococcus acidi lactici fermented solution;Described lactic acid bacteria, it is plant breast bar by re-activation One or more of any combination in bacterium, lactobacillus acidophilus, streptococcus thermophilus;
(3)Freezing:Fermentation seed is placed in into -34 DEG C to freeze 10 hours, takes out and is warming up to room temperature after 34 DEG C, makes kind of skin attachment Moisture is changed into the crystallization water, the tissue voids of increase kind skin, promotes external nourishment composition to immerse, stimulates plumule to grow, strengthen plumule Resistance, obtain freezing seed;
(4)Vernalization:Freezing seed is placed in germinating box, the temperature for adjusting germinating box is 24 DEG C, humidity 82%, stands vernalization 4 My god, obtain vernalization seed;
(5)Sowing:Vernalization seed is seeded in seedling medium, upper strata covering 0.6 ~ 1.1cm of plant ash, uniformly sprays step (2)In zymotic fluid, nutrition is balanced, promotes neutron absorption, accelerates plumule growth, improves seed resistance and environmental suitability, Percentage of seedgermination is improved, obtains sowing seed;Described seedling medium, it is made up of the raw material of following parts by weight:Corncob 46, eat Bacterium culture medium 37, fallen leaves 33, sawdust 24, biogas residue 21, vinasse 17, plant ash 10, egg shell 7, EM bacterium 5, vermiculite 17, perlite 13rd, slag ash 24, inorganic fertilizer 1.6, by corncob, culture medium of edible fungus, fallen leaves, sawdust, biogas residue, vinasse, plant ash and chicken Eggshell co-grinding, EM bacterium are accessed, compost fermentation 6 days, add vermiculite, perlite and slag ash, is well mixed, continues fermentation 3 My god, inorganic fertilizer is added, is well mixed, obtains seedling medium;Described inorganic fertilizer, it is made up of the raw material of following parts by weight:Urine Element 27, calcium superphosphate 21, potassium chloride 22, manganese sulfate 6, borax 4.3, zinc sulfate 1.6;
(6)Management:Sprinkled water daily, the water content for keeping plant ash is 42 ~ 44%, accelerates germination and is unearthed, sprout is unearthed After cover sunshade net, 3 days after being unearthed, sodium selenite solution that sprinkling mass concentration is 113mg/L, continuous sprinkling 2 times, during interval Between be 6 days, make seedling sturdy, avoid seedling from tanning severely, accelerate seedling early growth, carry out Routine Management afterwards, obtain Acer palmatum seedling.
Embodiment 2
A kind of spring method for culturing seedlings of Acer palmatum seed, comprises the following steps:
(1)Immersion:Before spring sowing, dry seed is placed in sodium hydroxide solution and soaked 4 hours, mass concentration 1.2%, To accelerate to destroy seed coat structure, the nutrition and moisture for promoting the external world are immersed, and are suppressed dormancy species activity, are accelerated germination, clean, Seed must be soaked;
(2)Fermentation:Immersion seed is placed in streptococcus acidi lactici fermented solution, fermented 28 hours in 42 DEG C, adjusts seed pH, promotes fermentation Caused a large amount of metabolite immerses kind of a skin, breaks seed dormancy, promote plumule growth, shortens the vernalization and after planting of seed Germinate unearthed time, filtering, obtains fermentation seed and zymotic fluid;Described streptococcus acidi lactici fermented solution, lactic acid bacteria is accessed into the training of MRS liquid Support in base, fermented 22 hours in 41 DEG C, obtain streptococcus acidi lactici fermented solution;Described lactic acid bacteria, it is plant breast bar by re-activation One or more of any combination in bacterium, lactobacillus acidophilus, streptococcus thermophilus;
(3)Freezing:Fermentation seed is placed in into -33 DEG C to freeze 11 hours, takes out and is warming up to room temperature after 35 DEG C, makes kind of skin attachment Moisture is changed into the crystallization water, the tissue voids of increase kind skin, promotes external nourishment composition to immerse, stimulates plumule to grow, strengthen plumule Resistance, obtain freezing seed;
(4)Vernalization:Freezing seed is placed in germinating box, the temperature for adjusting germinating box is 25 DEG C, humidity 83%, stands vernalization 4 My god, obtain vernalization seed;
(5)Sowing:Vernalization seed is seeded in seedling medium, upper strata covering 0.6 ~ 1.1cm of plant ash, uniformly sprays step (2)In zymotic fluid, nutrition is balanced, promotes neutron absorption, accelerates plumule growth, improves seed resistance and environmental suitability, Percentage of seedgermination is improved, obtains sowing seed;Described seedling medium, it is made up of the raw material of following parts by weight:Corncob 47, eat Bacterium culture medium 38, fallen leaves 34, sawdust 25, biogas residue 22, vinasse 18, plant ash 11, egg shell 8, EM bacterium 6, vermiculite 18, perlite 14th, slag ash 25, inorganic fertilizer 1.7, by corncob, culture medium of edible fungus, fallen leaves, sawdust, biogas residue, vinasse, plant ash and chicken Eggshell co-grinding, EM bacterium are accessed, compost fermentation 6 days, add vermiculite, perlite and slag ash, is well mixed, continues fermentation 3 My god, inorganic fertilizer is added, is well mixed, obtains seedling medium;Described inorganic fertilizer, it is made up of the raw material of following parts by weight:Urine Element 28, calcium superphosphate 22, potassium chloride 23, manganese sulfate 7, borax 4.4, zinc sulfate 1.7;
(6)Management:Sprinkled water daily, the water content for keeping plant ash is 42 ~ 44%, accelerates germination and is unearthed, sprout is unearthed After cover sunshade net, 4 days after being unearthed, sodium selenite solution that sprinkling mass concentration is 114mg/L, continuous sprinkling 2 times, during interval Between be 7 days, make seedling sturdy, avoid seedling from tanning severely, accelerate seedling early growth, carry out Routine Management afterwards, obtain Acer palmatum seedling.
Embodiment 3
A kind of spring method for culturing seedlings of Acer palmatum seed, comprises the following steps:
(1)Immersion:Before spring sowing, dry seed is placed in sodium hydroxide solution and soaked 5 hours, mass concentration 1.3%, To accelerate to destroy seed coat structure, the nutrition and moisture for promoting the external world are immersed, and are suppressed dormancy species activity, are accelerated germination, clean, Seed must be soaked;
(2)Fermentation:Immersion seed is placed in streptococcus acidi lactici fermented solution, fermented 30 hours in 43 DEG C, adjusts seed pH, promotes fermentation Caused a large amount of metabolite immerses kind of a skin, breaks seed dormancy, promote plumule growth, shortens the vernalization and after planting of seed Germinate unearthed time, filtering, obtains fermentation seed and zymotic fluid;Described streptococcus acidi lactici fermented solution, lactic acid bacteria is accessed into the training of MRS liquid Support in base, fermented 24 hours in 42 DEG C, obtain streptococcus acidi lactici fermented solution;Described lactic acid bacteria, it is plant breast bar by re-activation One or more of any combination in bacterium, lactobacillus acidophilus, streptococcus thermophilus;
(3)Freezing:Fermentation seed is placed in into -32 DEG C to freeze 12 hours, takes out and is warming up to room temperature after 36 DEG C, makes kind of skin attachment Moisture is changed into the crystallization water, the tissue voids of increase kind skin, promotes external nourishment composition to immerse, stimulates plumule to grow, strengthen plumule Resistance, obtain freezing seed;
(4)Vernalization:Freezing seed is placed in germinating box, the temperature for adjusting germinating box is 26 DEG C, humidity 84%, stands vernalization 5 My god, obtain vernalization seed;
(5)Sowing:Vernalization seed is seeded in seedling medium, upper strata covering 0.6 ~ 1.1cm of plant ash, uniformly sprays step (2)In zymotic fluid, nutrition is balanced, promotes neutron absorption, accelerates plumule growth, improves seed resistance and environmental suitability, Percentage of seedgermination is improved, obtains sowing seed;Described seedling medium, it is made up of the raw material of following parts by weight:Corncob 48, eat Bacterium culture medium 39, fallen leaves 35, sawdust 26, biogas residue 23, vinasse 19, plant ash 12, egg shell 9, EM bacterium 7, vermiculite 19, perlite 15th, slag ash 26, inorganic fertilizer 1.8, by corncob, culture medium of edible fungus, fallen leaves, sawdust, biogas residue, vinasse, plant ash and chicken Eggshell co-grinding, EM bacterium are accessed, compost fermentation 7 days, add vermiculite, perlite and slag ash, is well mixed, continues fermentation 4 My god, inorganic fertilizer is added, is well mixed, obtains seedling medium;Described inorganic fertilizer, it is made up of the raw material of following parts by weight:Urine Element 29, calcium superphosphate 23, potassium chloride 24, manganese sulfate 8, borax 4.5, zinc sulfate 1.8;
(6)Management:Sprinkled water daily, the water content for keeping plant ash is 42 ~ 44%, accelerates germination and is unearthed, sprout is unearthed After cover sunshade net, 5 days after being unearthed, sodium selenite solution that sprinkling mass concentration is 115mg/L, continuous sprinkling 2 times, during interval Between be 8 days, make seedling sturdy, avoid seedling from tanning severely, accelerate seedling early growth, carry out Routine Management afterwards, obtain Acer palmatum seedling.
Comparative example 1
Removal step(1), remaining method, with embodiment 1.
Comparative example 2
Removal step(2), remaining method, with embodiment 1.
Comparative example 3
Removal step(3), remaining method, with embodiment 1.
Comparative example 4
Step(4)In temperature keep 16 DEG C, remaining method, with embodiment 1.
Comparative example 5
Step(5)In seedling medium be changed to traditional matrices, remaining method, with embodiment 1.
Comparative example 6
Removal step(5)In covering plant ash, remaining method, with embodiment 1.
Comparative example 7
Removal step(5)In sprinkling step(2)In zymotic fluid, remaining method, with embodiment 1.
Comparative example 8
Removal step(6)In sodium selenite solution, remaining method, with embodiment 1.
Comparative example 9
Traditional spring method for culturing seedlings of existing Acer palmatum seed.
The nursery effect of embodiment and comparative example Acer palmatum seed seedling-raising method:
In the first area in Anhui provincial boundaries, 12000, the Acer palmatum seed of mature and plump is chosen, is randomly divided into 12 groups, every group 1000, The processing of Acer palmatum seed is carried out with the method for embodiment and comparative example respectively, is sowed in spring after processing, other management method phases Together, germination time, unearthed time and the germination percentage of each group Acer palmatum seed are recorded, is transplanted after 1 year, calculates transplanting survival The nursery effect of rate, embodiment and comparative example Acer palmatum seed seedling-raising method is shown in Table 1.
Table 1:The nursery effect of embodiment and comparative example Acer palmatum seed seedling-raising method
Project Germination time/(d) Unearthed time/(d) Germination percentage/(%) Transplanting success/(%)
Embodiment 1 4 8 93.9 98
Embodiment 2 4 9 94.7 100
Embodiment 3 5 7 94.2 99
Comparative example 1 9 11 87.3 95
Comparative example 2 14 16 86.2 92
Comparative example 3 10 12 87.7 93
Comparative example 4 6 9 90.5 96
Comparative example 5 8 10 87.8 97
Comparative example 6 7 11 88.3 96
Comparative example 7 7 12 91.5 98
Comparative example 8 5 8 94.1 92
Comparative example 9 146 22 83.5 91
Show from the result of table 1, the spring method for culturing seedlings of the Acer palmatum seed of embodiment, germination time and unearthed time substantially compared with Comparative example is short, and germination percentage and transplanting success are substantially high compared with comparative example, illustrate that the spring of Acer palmatum seed provided by the invention educates Seedling-growing method has good nursery effect.

Claims (6)

1. a kind of spring method for culturing seedlings of Acer palmatum seed, it is characterised in that comprise the following steps:
(1)Immersion:Before spring sowing, dry seed is placed in sodium hydroxide solution and soaked 4 ~ 5 hours, cleaned, kind must be soaked Son;
(2)Fermentation:Immersion seed is placed in streptococcus acidi lactici fermented solution, fermented 25 ~ 30 hours in 41 ~ 43 DEG C, filtering, must ferment kind Son and zymotic fluid;
(3)Freezing:Fermentation seed is placed in into -34 ~ -32 DEG C to freeze 10 ~ 12 hours, takes out and is warming up to room temperature after 34 ~ 36 DEG C, obtain Freeze seed;
(4)Vernalization:Freezing seed is placed in germinating box, the temperature for adjusting germinating box is 24 ~ 26 DEG C, humidity is 82 ~ 84%, quiet Put vernalization 4 ~ 5 days, obtain vernalization seed;
(5)Sowing:Vernalization seed is seeded in seedling medium, upper strata covering 0.6 ~ 1.1cm of plant ash, uniformly sprays step (2)In zymotic fluid, obtain sowing seed;
(6)Management:Sprinkled water daily, the water content for keeping plant ash is 42 ~ 44%, and sprout covers sunshade net after being unearthed, and is unearthed 3 ~ 5 days afterwards, the sodium selenite solution that sprinkling mass concentration is 113 ~ 115mg/L was continuous to spray 2 times, and interval time is 6 ~ 8 days, Routine Management is carried out afterwards, obtains Acer palmatum seedling.
2. the spring method for culturing seedlings of Acer palmatum seed according to claim 1, it is characterised in that the step(1)Hydrogen-oxygen Change sodium solution, mass concentration is 1.1 ~ 1.3%.
3. the spring method for culturing seedlings of Acer palmatum seed according to claim 1, it is characterised in that the step(2)Lactic acid Fermented liquid, lactic acid bacteria is accessed in MRS fluid nutrient mediums, fermented 20 ~ 24 hours in 40 ~ 42 DEG C, obtain streptococcus acidi lactici fermented solution.
4. the spring method for culturing seedlings of Acer palmatum seed according to claim 3, it is characterised in that described lactic acid bacteria, pass through Re-activation is crossed, is one or more of any combination in Lactobacillus plantarum, lactobacillus acidophilus, streptococcus thermophilus.
5. the spring method for culturing seedlings of Acer palmatum seed according to claim 1, it is characterised in that the step(5)Nursery Matrix, it is made up of the raw material of following parts by weight:Corncob 46 ~ 48, culture medium of edible fungus 37 ~ 39, fallen leaves 33 ~ 35, sawdust 24 ~ 26th, biogas residue 21 ~ 23, vinasse 17 ~ 19, plant ash 10 ~ 12, egg shell 7 ~ 9, EM bacterium 5 ~ 7, vermiculite 17 ~ 19, perlite 13 ~ 15, stove Slag ash 24 ~ 26, inorganic fertilizer 1.6 ~ 1.8, by corncob, culture medium of edible fungus, fallen leaves, sawdust, biogas residue, vinasse, plant ash and Egg shell co-grinding, EM bacterium are accessed, compost fermentation 6 ~ 7 days, add vermiculite, perlite and slag ash, is well mixed, continues to send out Ferment 3 ~ 4 days, inorganic fertilizer is added, be well mixed, obtain seedling medium.
6. the spring method for culturing seedlings of Acer palmatum seed according to claim 5, it is characterised in that described inorganic fertilizer, by The raw material composition of following parts by weight:Urea 27 ~ 29, calcium superphosphate 21 ~ 23, potassium chloride 22 ~ 24, manganese sulfate 6 ~ 8, borax 4.3 ~ 4.5th, zinc sulfate 1.6 ~ 1.8.
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CN109121965A (en) * 2018-08-23 2019-01-04 江苏绿苑园林建设有限公司 A kind of seeding growing seedlings method of Acer palmatum
CN112931009A (en) * 2021-02-19 2021-06-11 山东绿韵农林科技发展股份有限公司 Seedling raising method for pterocarpus santalinus seeds
CN113439761A (en) * 2020-03-25 2021-09-28 云南水木良田农业科技有限公司 Sprouting vegetable planting bacterium paste and application

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109121965A (en) * 2018-08-23 2019-01-04 江苏绿苑园林建设有限公司 A kind of seeding growing seedlings method of Acer palmatum
CN113439761A (en) * 2020-03-25 2021-09-28 云南水木良田农业科技有限公司 Sprouting vegetable planting bacterium paste and application
CN113439761B (en) * 2020-03-25 2023-01-10 云南水木良田农业科技有限公司 Sprouting vegetable planting bacterium paste and application
CN112931009A (en) * 2021-02-19 2021-06-11 山东绿韵农林科技发展股份有限公司 Seedling raising method for pterocarpus santalinus seeds
CN112931009B (en) * 2021-02-19 2022-04-05 山东绿韵农林科技发展股份有限公司 Seedling raising method for pterocarpus santalinus seeds

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