CN105519278A - Application of vegetable amino acid fertilizer in seed germination of Magnolia zenii - Google Patents
Application of vegetable amino acid fertilizer in seed germination of Magnolia zenii Download PDFInfo
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- CN105519278A CN105519278A CN201410522240.8A CN201410522240A CN105519278A CN 105519278 A CN105519278 A CN 105519278A CN 201410522240 A CN201410522240 A CN 201410522240A CN 105519278 A CN105519278 A CN 105519278A
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Abstract
The invention discloses an application of a vegetable amino acid fertilizer in seed germination of Magnolia zenii. The application comprises the following steps: (1) removing aril from seeds of Magnolia zenii by adopting a seed retting method; (2) disinfecting the seeds for 15 minutes by adopting a 0.5% potassium permanganate solution; and cleaning the seeds, then, putting the cleaned seeds in a refrigerator with the temperature of 4 DEG C, and carrying out stacked stratification for 90 days; (3) preparing a 5-100mg/L GGR solution by taking distilled water as a solvent; and submerging the seeds in the GGR solution for 24 hours; and (4) carrying out seed germination on the drug-treated seeds by adopting a sand culture method under the constant condition of 15 DEG C. According to the application, the action of promoting the germination of the Magnolia zenii seeds is obvious by using GGR solutions with the concentration of 20mg/L and 10mg/L, the percentage of seed germination separately reaches 74% and 73%, and the germination energy separately reaches 27% and 52%, so that the germinating characteristic of the seeds are remarkably improved.
Description
Technical field
The present invention relates to a kind of method promoting seed sprouting, relate to the germinating method of precious magnificent yulan (MagnoliazeniiCheng) seed specifically, belong to agricultural technology field.
Background technology
Precious magnificent yulan (MagnoliazeniiCheng) is the endemic plant of China, first-grade state protection plant.Be distributed in the ground such as the Jiangsu of China's Mainland, be grown on the area of height above sea level 220m, be grown on hilly ground more, not yet by artificial introducing and planting.This kind of leaf shape of falling ovum Long Circle, tepal cochlear, the features such as the following purple of outer Middle face and sibling species yulan magnolia have obvious difference.This kind of flower diameter 12cm, fragrance is gorgeous, is the front yard circle ornamental tree species of grace.
This kind originates in industry's Baohua Mountain of Jurong, southern Jiangsu, and 18 strains are only deposited in the place of production, and urgency in imminent danger has reached the edge of extinction.The precious Huashan divides nature reserve.Distinguish obviously with its sibling species class for this kind, the categorizing system for research Magnolia has certain meaning.
Precious magnificent yulan is Magnoliaceae Magnolia deciduous tree, and up to 11m, the diameter of a cross-section of a tree trunk 1.3 meters above the ground reaches 30cm, bark canescence, smoothly.Spray is green, and without hair, old branch purple, dredges rawhide hole; Bud is narrow avette, and top is slightly curved, by long thin,tough silk hair.Leaf film quality, the shape of falling ovum Long Circle or Long Circle, long 7-16cm, wide 3-7cm, the wide round tool acumen of tip, the wealthy wedge shape of base portion or circle blunt, above green, without hair, below light green, middle arteries and veins and lateral vein have long curved hairs, lateral vein every limit 8-10 bar; The long 0.6-1.8cm of petiole, first villosity, the long 1/5-1/2 long for petiole of stipule trace.Bud is avette, and Hua Xianye is open, and have fragrance, diameter is about 12cm; The long 2-4mm of bennet, is closely become mildewed; Tepal 9, nearly cochlear, tip circle or slightly point, long 6.8-7.8cm, wide 2.7-3.8cm, inside take turns narrower and small, white, following lilac red in the middle part of the back side, top white; Stamen is about 11mm, and flower pesticide is about 7mm, and separately, interior side direction ftractures two coyote holes, and connective stretches out into short point, and filigree purple, is about 4mm; Gynoecium is cylindrical, is about 2cm, and carpel is about 4mm, and style is about 1mm.Aggregate fruit is cylindrical, long 5-7cm; Cheng Shu Follicle radish subcircular, has wart point-like protruding, the blunt circle in top.The florescence 3-4 month, the fruit phase 8-9 month.(Chinese Academy of Sciences's " Chinese Plants will " editorial board. Chinese Plants will [M]. Beijing: Science Press, 1996.30 (1))
The seed of Magnoliacea plant has after-ripening hibernation feature, cause emerge during seeding and seedling raising irregular.(.2014 such as Shen Chao, Wang Ying, Zhang Qianyun, the dynamic change [J] of endosperm storage material and enzymic activity in two Qiao Yulan Seed Germinations. forestry science and technology develops .28 (2): 55-58)
Kindred plant seed structure is different, and complicated physiological change occurs during germination, and its Germination Physiology characteristic of different plant also different (Pan Ruichi, Dong Yu obtain. plant physiology [M], Higher Education Publishing House, 1998).
The magnificent yulan seminal propagation of treasured be have not been reported.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of comprehensive utilization earthing of seeds sand Tibetan, the seed soaking of plant amino acid fertilizer, the temperature controlled technical measures of seed sprouting, improves the method for precious magnificent yulan seed sprouting.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is: the application of a kind of plant amino acid fertilizer in the magnificent yulan seed sprouting of treasured.
Described plant amino acid fertilizer is two gills.
Above-mentioned application comprises the following steps:
1) kind of the precious magnificent yulan arillus of method removal is macerated in employing;
2) potassium permanganate (KMnO of 0.5% is adopted
4) solution disinfection seed 15min; Put into 4 DEG C of refrigerators after cleaning, lamination sand hides 90d;
3) take distilled water as solvent, be mixed with two gill (GGR) solution of 5-100mg/L; Seed is immersed 24h in two gill (GGR) solution;
4) seed chemicals treatment crossed adopts sandy culture (S), under 15 DEG C of constant temperatures, carry out seed sprouting.
In the present invention, the concentration of described pair of gill (GGR) solution is 5mg/L to 100mg/L.
Optium concentration is 20mg/L and 10mg/L.
The beneficial effect that the present invention reaches: the present invention is the germination characteristic being improved precious magnificent yulan seed by the mutual synergy between two gill seed soaking, Stratification time, germination temperature.Under the comprehensive function of above measure, concentration is that two gills (GGR) solution of 20mg/L and 10mg/L promotes that the sprouting effect of precious magnificent yulan seed is obvious, percentage of seedgermination reaches 74% and 73% respectively, and germination vigor reaches 27% and 52% respectively, significantly improves the germination characteristic of seed.
Accompanying drawing explanation
Fig. 1 is precious magnificent yulan seed sprouting tendency chart after employing the present invention.
Embodiment
Magnoliaceae magnolia original seed generally adopts the method for seminal propagation.Two gill (GGR) the multiple concentration of plant amino acid fertilizer is used to process the magnificent yulan seed of treasured in the present embodiment, the suitable concentration being intended to explore this plant amino acid fertilizer on the impact of the magnificent yulan Germination Characteristics of treasured, to for the magnificent yulan plant germplasm resource of wild treasured preservation, introduce a fine variety and to cultivate and the breeding research of magnolia provides foundation.
One, for examination material
Precious magnificent yulan seed picks up from industry's Baohua Mountain of Jurong, southern Jiangsu, and seed collecting maternal plant is the magnificent yulan adult plants of the wild treasured in the precious Huashan.Thousand grain weigth is 136.24g.
Two, medicament
Two gill (GGR)
Two gill-GGR: common name (amino acid fertilizer)
Registration card number: fertile No. 1139, the accurate word of agriculture
Operative norm: Q/CPABT002-2010
Active ingredient: amino acid >=20.0%, trace element >=2.0%
Beijing Ai Bidi research and development centre
The preparation of reagent
Be solvent with distilled water, be mixed with two gills (GGR) solution that concentration is 5mg/L, 10mg/L, 20mg/L, 50mg/L, 100mg/L.
Three, seed treatment
The treasured China yulan seed of gathering fully ripe, adopts and macerates kind of a method removing arillus; Wet cleaning process is adopted to remove flat grain; Employing mass concentration is the potassium permanganate (KMnO of 0.5%
4) solution disinfection seed 15min, put into 4 DEG C of refrigerators after cleaning, lamination sand hides 90d;
Four, test method
Treated seed being immersed 24h in two gills (GGR) solution of concentration 5mg/L, 10mg/L, 20mg/L, 50mg/L, 100mg/L, take clear water as contrast; Seed chemicals treatment crossed adopts sandy culture (S), carries out seed germination experiment, daily iron supplement moisture under 15 DEG C of constant temperatures, keeps water content of substrate at 60-80%, observes seed sprouting situation.
Test often processes repetition 3 times, often repeats 50 seeds.
After planting, growing to seed length for germinateing with radicle, to terminate for germinateing without germinateing in seed sprouting to 7 day, every day entry chitting piece number.
Germination rate (%)=total grain number that germinates/test total grain number × 100%
Germination vigor (%)=alloted days (1/3 germination number of days) interior chitting piece number/test total grain number × 100%
Test data adopts SPSS statistical analysis software to carry out the one-factor analysis of variance.With least significant difference method, significance test is carried out to germination rate and germination vigor data.
Five, interpretation of result
1. pair gill (GGR) solution seed soaking is on the impact of the magnificent yulan percentage of seedgermination of treasured
Note: in same column data, letter (small letter) different person represents significant difference (P<0.05); In same column data, letter (capitalization) different person represents that difference extremely significantly (P<0.01);
Show from the two the results of analysis of variance of gill (GGR) solution seed soaking to treasured magnificent yulan percentage of seedgermination of variable concentrations: concentration is that two gills (GGR) solution of 20mg/L and 10mg/L promotes that the effect of Goebel China yulan seed germination is obvious, percentage of seedgermination reaches 74% and 73% respectively, be significantly higher than clear water contrast (CK) germination rate 57%, and reach pole significance level.
2. pair gill (GGR) solution seed soaking is on the impact of the magnificent yulan potentiality of seed of treasured
(1) germination vigor variance analysis
Note: in same column data, letter (small letter) different person represents significant difference (P<0.05); In same column data, letter (capitalization) different person represents that difference extremely significantly (P<0.01);
Show from the two the results of analysis of variance of gill (GGR) solution seed soaking to treasured magnificent yulan potentiality of seed of variable concentrations: concentration is that two gills (GGR) solution of 10mg/L, 5mg/L, 100mg/L, 20mg/L promotes that the effect of Goebel China yulan potentiality of seed is obvious, potentiality of seed reaches 52%, 50%, 36%, 27% respectively, be significantly higher than clear water contrast (CK) germination vigor 18%, and reach pole significance level.
(2) germination rate (3 days) trend analysis
As shown in Figure 1, adopt the treasured China yulan seed of two gill (GGR) solution seed soaking of variable concentrations after planting, initially to terminate for germinateing to the 40th day for germinateing with the 16th day, calculating germination rate with every 3 days chitting piece numbers, being depicted as precious magnificent yulan seed sprouting tendency chart.
As can be seen from tendency chart: concentration is that two gills (GGR) the solution seed soaking process of 10mg/L, 5mg/L, 100mg/L promotes the successful that precious magnificent yulan seed sprouting peak shifts to an earlier date.
Six, conclusion
The germination test result of the precious magnificent yulan seed of two gill (GGR) solution seed soaking of comprehensive variable concentrations can draw to draw a conclusion: two gills (GGR) solution utilizing concentration to be 20mg/L and 10mg/L promotes that the sprouting effect of precious magnificent yulan seed is obvious, percentage of seedgermination reaches 74% and 73% respectively, germination vigor reaches 27% and 52% respectively, significantly carries the germination characteristic of seed.Above effect is realized by the mutual synergy between two gill concentration, Stratification time, germination temperature.
Conclusions illustrates that the reasonable concentration of the two gill (GGR) of plant amino acid fertilizer significantly can improve the germination characteristic of precious magnificent yulan seed.
Claims (5)
1. the application of plant amino acid fertilizer in the magnificent yulan seed sprouting of treasured.
2. application according to claim 1, is characterized in that, described plant amino acid fertilizer is two gills.
3. application according to claim 2, is characterized in that, comprises the following steps:
1) kind of the precious magnificent yulan arillus of method removal is macerated in employing;
2) employing mass concentration is the disinfecting solution of potassium permanganate seed 15min of 0.5%; Put into 4 DEG C of refrigerators after cleaning, lamination sand hides 90d;
3) take distilled water as solvent, be mixed with the two gill solution of 5-100mg/L; Seed is immersed 24h in two gill (GGR) solution;
4) seed chemicals treatment crossed adopts sandy culture (S), under 15 DEG C of constant temperatures, carry out seed sprouting.
4. application according to claim 3, is characterized in that: the concentration of described pair of gill (GGR) solution is 5mg/L to 100mg/L.
5. application according to claim 4, is characterized in that: the concentration of described pair of gill (GGR) solution is 20mg/L and 10mg/L.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106962094A (en) * | 2017-02-27 | 2017-07-21 | 焦杰彪 | A kind of Magnolia wufengensis seeding growing seedlings method |
| CN111448988A (en) * | 2020-05-18 | 2020-07-28 | 江苏农林职业技术学院 | Culture method and culture medium for inhibiting endophyte pollution in primary culture of magnolia zenii |
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| CN103430713A (en) * | 2013-06-25 | 2013-12-11 | 句容市仑山花木场 | Efficient magnolia grandiflora culture method |
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2014
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20120086947A (en) * | 2011-01-27 | 2012-08-06 | 주식회사 함소아제약 | Oriental medicine composition for curing rhinitis in children and manufacturing method thereof |
| CN103430713A (en) * | 2013-06-25 | 2013-12-11 | 句容市仑山花木场 | Efficient magnolia grandiflora culture method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106962094A (en) * | 2017-02-27 | 2017-07-21 | 焦杰彪 | A kind of Magnolia wufengensis seeding growing seedlings method |
| CN111448988A (en) * | 2020-05-18 | 2020-07-28 | 江苏农林职业技术学院 | Culture method and culture medium for inhibiting endophyte pollution in primary culture of magnolia zenii |
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