CN101904236A - Sprouting method of rapidly removing pyrus betulaefolia seeds from dormancy by using 2-thiopseudourea - Google Patents
Sprouting method of rapidly removing pyrus betulaefolia seeds from dormancy by using 2-thiopseudourea Download PDFInfo
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- CN101904236A CN101904236A CN2010101897552A CN201010189755A CN101904236A CN 101904236 A CN101904236 A CN 101904236A CN 2010101897552 A CN2010101897552 A CN 2010101897552A CN 201010189755 A CN201010189755 A CN 201010189755A CN 101904236 A CN101904236 A CN 101904236A
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Abstract
The invention provides a method for rapidly removing pyrus betulaefolia seeds from dormancy to promote sprouting thereof, and relates to a method for removing seed dormancy and a seed sprouting technology, belonging to the technical field of plant production. The invention mainly draws on a characteristic that a chemical agent 2-thiopseudourea can break up seed embryo dormancy to remove pear seeds from seed embryo dormancy so as to promote the pear seeds to germinate, improve sprouting rate and tidy the sprouting. The sprouting method by using the 2-thiopseudourea comprises the following three steps: (1) peeling seedcases of ripe fruits, washing by hands and removing pulps, using NaClO with the mass volume ratio of 20% to soak and treat the seeds to remove the pulps adhered on the seedcases; (2) soaking for 8 min by using a mercury bichoride solution with the mass volume ratio of 0.1% for sterilization, taking the clean pyrus betulaefolia seeds to be respectively placed in a 2-thiopseudourea solution with the mass volume ratio of 0.8% to soak for 24h at room temperature; and (3) washing the soaked pyrus betulaefolia seeds for five-six times by fresh water, and then seeding.
Description
Technical field
The present invention is with the germination accelerating method of different sulphur guanidine urea quickly breaking dormancy of birch-leaf pear seeds, belongs to botany, seed science and field of ecology.
Background technology
The pears seed dormancy is meant allly have that vigor is in again under the suitable sprouting condition and the residing state of ablastous pears seed.With regard to the pears seed itself, it is an important vital movement process, is again a kind of useful biological property, is a kind of adaptation to various factorss such as ecotope, climatic variation and temp. and humidity differences that pears obtain through long-term evolution.The pears seed dormancy is being represented a kind of important protected mode, is to regulate the Best Times of pears seed germination and the effective ways in space, guarantees the pears seedling survival, and this is also beneficial to the mankind.Pears need could sprout through one period resting stage after gathering, cause certain difficulty often for production and scientific research, the pears seed of dormancy might make the shortage of growing seedlings if shortage is handled in advance or time, the method for processing are improper, the afforestation predetermined scheme postpones, and brings heavy losses.In real work, generally adopt the lamination method to remove the pears seed dormancy.So-called lamination handle be seed is mixed with wet matters such as wet sand or wet peat or layering be placed on store 40～60 days breaking dormancies under the condition of low temperature, moistening, ventilation after, sow the ability rudiment.Seed often because of holding bad moisture, aeration condition or lamination time, easily influences the normal breaking dormancy of seed during sand is hidden, cause after planting seed not germinate or germination rate low.For addressing the above problem, on the basis of verifying pears seed dormancy reason and sprouting condition, search out a kind of germination accelerating method fast and effectively---different sulphur guanidine urea method.
Summary of the invention
Technical problem the purpose of this invention is to provide the germination accelerating method of birch-leaf pear seed, and raising potentiality of seed, germination rate impel early seedling, strong sprout, Qi Miao, and producing upward with solution, the stock seed must carry out the problem that long-time low temperature lamination processing could be sprouted.
Technical scheme the invention provides a kind of new birch-leaf pear seed quick pregermination method, and its seed does not need the lamination processing just can sprout.Concrete steps are as follows:
1) collection of seed: the pericarp of ripening fruits is peeled off, wash rejecting pulp by rubbing with the hands, stay the seed that contains endocarp, remove the inferior strain of keeping afloat with wet cleaning process, select full, unabroken seed, then with 20%NaCl immersion treatment seed to remove the pulp that adheres on kind of the skin, take out the back and brush away kind of the impurity on skin surface (but can not damage the surface texture that forms naturally in the seed development) gently, seed is shakeout place indoor natural seasoning with writing brush.
2) seed suction: the dry seed that will dry is put into water and is soaked, and seed soaking 1h fully absorbs water seed and reaches capacity.
3) chemical agent is handled: the birch-leaf pear seed is soaked the 8min sterilization with 0.1% mercuric chloride solution, after flushing with clean water 5～6 times, seed is placed the 0.8% different sulphur guanidine urea solution 24h that soaks seed respectively, then with running water seed is washed 5～6 times, be seeded in the soil.
4) sowing: the every 667m2 sowing of birch-leaf pear seed 1.5～2kg, with double rows line seeding (the narrow row line-spacing is 30～50cm, wide every trade apart from be 60～70cm) or the single file drilling (line-spacing 50～60cm) is opened the shallow ridges of dark 2～3cm sowing time earlier, seed evenly is sprinkled into, and earthing gets final product then.
5) broadcast back management: do not water before emerging, with the immersion of wide row trench digging or on seed furrow, spray water, emerge and carry out intertill and clean tillage after showing row, carry out final singling during the straight leaf of seedling length 5～7cm as soil drought.About spacing in the rows 10cm, the place of being short of seedling in time benefit plants and waters immediately.
Beneficial effect
Good effect of the present invention:
1) the present invention finds first that the birch-leaf pear seed is handled without the low temperature lamination and just can remove its seed dormancy.Therefore Dormancy Mechanism research not only there is theoretic meaning, and important practical value is arranged.Application the present invention can study seed and be broken later metabolic activity with dormancy in the breaking dormancy process.
2) use method of the present invention, promoted the birch-leaf pear seed germination, for physiological change processes such as the hydrolysis conversion of the imbibition in the further investigation birch-leaf pear seed germination process, internal substance and cell division regeneration are laid a good foundation.
3) the present invention is based on the working foundation to characteristic research such as the physiological and biochemical procedure of pears seed dormancy and sprouting and rules thereof.People have grasped the pears dormancy characteristics of different, use this research control and utilize the pears seed dormancy, serve production of agricultural gardening and plant introduction domestication.
4) in the seed testing process, must adopt proper method to abolish the pears seed dormancy, use vigor, germinating capacity that the present invention can measure dormancy pears seed rapidly and accurately, so that guiding agricultural production correctly.
5) the sowing quality of seed is first factor of decision seedling quality.Use this research, the reparation before quickening to germinate, sowing in time, then the pears potentiality of seed is good, the germination rate height, seedling early, strong sprout is many, and seedling is neat, help seedling to the competitions of weeds with to the utilization of daylight, soil, moisture and nutritional condition, and reduce the loss that causes owing to being short of seedling.
Description of drawings
Fig. 1 birch-leaf pear seed collection is to the flow chart of sowing
Fig. 2 birch-leaf pear seed sprouting situation in incubator
Fig. 3 birch-leaf pear seed is sprouted situation big Tanaka
Embodiment
Removing seed dormancy, is research seed dormancy reason and one of Mechanism of Dormancy, sprouting condition and the physiological prerequisite of sprouting.
By vernalization, can break Seed Dormancy, strengthen seed internal physiology activity (respiration of the activity of enzyme, the conversion of nutrient, embryo etc.), promote the sprouting of seed, improve percentage of seedgermination, make to come up neatly, shorten the seeding stage, and improve seedling quality and qualified seedling output, for the nursery stock good quality and high output lays the foundation.The processing of low temperature lamination is the breaking birchleaf pear seed dormancy, impels the effective means of seed germination, but moisture during the lamination and oxygen are difficult for grasping; In addition, the lamination overlong time needs 40～60 days.Use method of the present invention, solution concentration and seed soaking time are easily grasped, just showed money or valuables one carries unintentionally in after planting the 3rd day, and be the most simple, fast a kind of vernalization technology, its specific operation process is as follows:
The collection of 1 seed: the pericarp of ripening fruits is peeled off, wash rejecting pulp by rubbing with the hands, stay the seed that contains endocarp, remove the inferior strain of keeping afloat with wet cleaning process, select fullly, unabroken seed uses 20% clorox (NaClO) bubble to handle seed to remove the pulp that adheres on kind of the skin then, take out the back and brush away kind of the impurity on skin surface (but can not damage the surface texture that forms naturally in the seed development) gently, place indoor natural seasoning with writing brush.
The seed that 2 go bail for deposits, the 1h of clear water seed soaking at room temperature soaks the 8min sterilization with 0.1% mercuric chloride solution then, then uses the flushing with clean water seed.
3 place the solution of the 0.8% different sulphur guanidine urea 24h that soaks seed respectively with seed,, be seeded in culture dish or the soil seed flushing 5～6 times with running water, and after the seed soaking 3 days, seed begins to show money or valuables one carries unintentionally, and a week has sprouted, and germination rate reaches 75%, and emergence rate is 66%.(Fig. 2 and 3).
Claims (1)
1. with the germination accelerating method of different sulphur guanidine urea quickly breaking dormancy of birch-leaf pear seeds, comprise the following step:
1) collection of birch-leaf pear seed:
The pericarp of ripening fruits is peeled off, wash rejecting pulp by rubbing with the hands, stay the seed that contains endocarp, remove the inferior strain of keeping afloat with wet cleaning process, select full, unabroken seed is planted the pulp that adheres on the skin with mass volume ratio 20% clorox NaClO immersion treatment seed to remove then, seed is shakeout place indoor natural seasoning;
2) clear water seed soaking and seed disinfection:
(1) seed of going bail for and depositing, the 1h of clear water seed soaking at room temperature;
(2) soak the 8min sterilization with mass volume ratio 0.1% mercuric chloride solution, then use the flushing with clean water seed;
3) chemical agent seed soaking and seed clean:
(1) chemical agent seed soaking: get the birch-leaf pear seed that cleans up, at ambient temperature, put into the mass volume ratio 0.8% different sulphur guanidine urea 24h that soaks seed respectively;
(2) flushing with clean water 5～6 times of the birch-leaf pear seed after the seed soaking;
4) sowing:
Seed is placed on culture dish or is seeded into the land for growing field crops after cleaning 5～6 times.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101897552A CN101904236A (en) | 2010-06-02 | 2010-06-02 | Sprouting method of rapidly removing pyrus betulaefolia seeds from dormancy by using 2-thiopseudourea |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010101897552A CN101904236A (en) | 2010-06-02 | 2010-06-02 | Sprouting method of rapidly removing pyrus betulaefolia seeds from dormancy by using 2-thiopseudourea |
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| CN101904236A true CN101904236A (en) | 2010-12-08 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104067726A (en) * | 2014-07-11 | 2014-10-01 | 任晓萍 | Seed growing method for pyrus betulaefolia |
| CN106973581A (en) * | 2017-04-07 | 2017-07-25 | 扬州大学 | A kind of method for releasing pears seed dormancy |
| CN107223381A (en) * | 2017-05-17 | 2017-10-03 | 何子顺 | A kind of quick method for producing birch-leaf pear seed |
| CN111280009A (en) * | 2020-03-20 | 2020-06-16 | 四川省农业科学院经济作物育种栽培研究所 | Peanut seedling early sowing method |
| CN111742640A (en) * | 2020-06-08 | 2020-10-09 | 山东省林业科学研究院 | Germination accelerating method for birch pear seeds |
-
2010
- 2010-06-02 CN CN2010101897552A patent/CN101904236A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104067726A (en) * | 2014-07-11 | 2014-10-01 | 任晓萍 | Seed growing method for pyrus betulaefolia |
| CN104067726B (en) * | 2014-07-11 | 2016-06-01 | 任晓萍 | The seed seedling-raising method of a kind of birch-leaf pear |
| CN106973581A (en) * | 2017-04-07 | 2017-07-25 | 扬州大学 | A kind of method for releasing pears seed dormancy |
| CN107223381A (en) * | 2017-05-17 | 2017-10-03 | 何子顺 | A kind of quick method for producing birch-leaf pear seed |
| CN111280009A (en) * | 2020-03-20 | 2020-06-16 | 四川省农业科学院经济作物育种栽培研究所 | Peanut seedling early sowing method |
| CN111742640A (en) * | 2020-06-08 | 2020-10-09 | 山东省林业科学研究院 | Germination accelerating method for birch pear seeds |
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Application publication date: 20101208 |