CN105660237A - Method for shortening juvenile period of directive breeding hybrid seedlings of Pyrus bretschneideri Rehd. - Google Patents
Method for shortening juvenile period of directive breeding hybrid seedlings of Pyrus bretschneideri Rehd. Download PDFInfo
- Publication number
- CN105660237A CN105660237A CN201610061391.7A CN201610061391A CN105660237A CN 105660237 A CN105660237 A CN 105660237A CN 201610061391 A CN201610061391 A CN 201610061391A CN 105660237 A CN105660237 A CN 105660237A
- Authority
- CN
- China
- Prior art keywords
- cross hybrid
- hybrid seedling
- kuerle delicious
- delicious pear
- cross
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for shortening the juvenile period of directive breeding hybrid seedlings of Pyrus bretschneideri Rehd. The method adopts sowing and greenhouse seedling raising in the same year, and comprises the following steps: removing seed coat, carrying out gibberellin treatment, spraying GA3 in the growing period of the hybrid seedlings, conducting inversed ring-bark grafting on the hybrid seedlings sprayed with GA3, carrying out hybrid seedling terminal bud grafting, dwarfing stock, spraying chlormequat chloride (CCC), and various other technical measures, so as to shorten the juvenile period of the directive breeding hybrid seedlings of Pyrus bretschneideri Rehd. According to the method, the Pyrus bretschneideri Rehd. hybrid seedlings can bloom and fruit in the third year after being planted, and the fruiting plant rate can be 30 percent, while the conventional Pyrus bretschneideri Rehd. hybrid seedlings bloom and fruit 7 to 8 years after being planted. Being treated with the six measures, the Pyrus bretschneideri Rehd. directive breeding hybrid seedlings can fruit in the third year after being planted, that is, the juvenile period is shortened by 4 to 5 years. Therefore, the Pyrus bretschneideri Rehd. directive breeding efficiency is greatly improved; the Pyrus bretschneideri Rehd. directive breeding period is shortened; a new way and a new method are provided for breeding of new Pyrus bretschneideri Rehd. directive breeding varieties; the application value is high.
Description
Invention field
The present invention relates to the technical field of plant breeding. Specifically, the present invention relates to the technical field of Kuerle delicious pear directive breeding hybridization.
Background technology
Kuerle delicious pear (PyrusbretschneideriRehd) belongs to the Rosaceae (Rosaceae), pears subfamily (Pomoideae), pears belong to white pear system in (Pyrus), Uighur name is that sago is carried or is Xi Puti, by name the opening of Mongolian steps on wood, originate in Kurle region, Xinjiang, the cultivation history of existing more than 1400 year in Xinjiang, its fruit aromatic flavour, the thin meat of skin is crisp, fresh and sweet tasty and refreshing, delicate succulence, keeping quality is strong, it is one of China four your name's pears, end 2014, the cultivation total area of Xinjiang Kuerle delicious pear reaches 98.87 ten thousand mu, ultimate production reaches 104.48 ten thousand tons. Kuerle delicious pear industry has become the leading industry of Kurle region, Kuerle delicious pear is the longest " Pear varieties that domestic pears selling price is the highest of China's supermarket occupation rate the highest " sales range is the widest " selling time at home, Kuerle delicious pear has the good reputation of " China's honey pears ", " king in fruit ", " in pears prince ", " after pears " in the international market. product finds a good sale in multiple countries and regions such as Thailand, Japan, Canada, Singapore, Malaysia, dark liking by domestic and international human consumer, cultivation through over a long time is bred, the continuous change of weather condition, the generation of the weather of extreme low temperature and high temperature, the quality of Kuerle delicious pear is constantly degenerated, and place calyx fruit (calyx fruit in place i.e. public pears, de-calyx fruit i.e. female pears), prominent calyx fruit and tertia fruit are on the increase, core becomes big, stone cell increases, and canker and freeze injury be the problem such as generation often, as: 2010/2011 year winter the fragrant disaster-stricken total area 10241hm of pears freeze injury of Korla City2, wherein endure cold heavy orchard area 4350.5hm2, the orchard area 2703hm enduring cold medium2, the orchard area 3186.8hm enduring cold lighter2, the underproduction about 75%, direct economic loss 7.18 hundred million yuan.
The current Problems existing of Kuerle delicious pear, seriously have impact on the quality of Kuerle delicious pear, output and sale, greatly reduces the economic benefit of Kuerle delicious pear and the income of orchard worker. Therefore, need badly and Kuerle delicious pear is carried out directive breeding, select the Kuerle delicious pear new variety of applicable current climatope condition, could fundamentally solve the current Problems existing of Kuerle delicious pear, increase the fragrant economic benefit of pears and the income of orchard worker, simultaneously to driving the sustainable and stable development of fragrant pears industry, promote superior resources to transform, promote that the economic prosperity of minority area has fairly obvious realistic meaning.
During pear tree breeding, the problems such as the Tong Qichang of cross hybrid seedling, breeding cycle length, difficulty are big, cost height, add that geographical position, Xinjiang, weather condition are more special, time in winter is long, the existence of the problems such as soil salinity and alkalinity height, causes that the virgin phase of Kuerle delicious pear directive breeding cross hybrid seedling is longer, breeding cycle is longer, cost is more high. The cross hybrid seedling of traditional Kuerle delicious pear directive breeding, generally needs 8 to 10 years results, more than 10 year ability result under Special Circumstances, as: new No. 1, pears, hybridization pollination in 1974, within 1993, by variety certification, breeding time reaches 19 years; No. 6, new pears, nineteen eighty-two hybridization pollination, within 1997, breeding time reaches 15 years by variety certification; New type deposit, nineteen eighty-three hybridization pollination, in September, 2001 by variety certification, No. 8, new pears, breeding time reaches 18 years; Hybridization pollination in 1984, in December, 2010, breeding time reached 16 years by variety certification; These reach the breeding time of nearly 20 years, and the time having 1/2 causes by the virgin phase length of cross hybrid seedling. These longer breeding time bring very big difficulty to the directed cross-breeding of Xinjiang Kuerle delicious pear, hit enthusiasm and enthusiasm that scientific research personnel is engaged in Kuerle delicious pear directive breeding, seriously hinder the development of the health problem of the fragrant pears industry of development and Xinjiang of Kuerle delicious pear directive breeding scientific research. Therefore the virgin phase of research shortening Kuerle delicious pear directive breeding cross hybrid seedling has the meaning of reality.
Summary of the invention
For the Tong Qichang of cross hybrid seedling during at present cross-breeding directed for Kuerle delicious pear, breeding cycle length, the state of the art that cost is high, the present invention is intended to provide the method for the virgin phase shortening Kuerle delicious pear directive breeding cross hybrid seedling, and the inventive method make Kuerle delicious pear directive breeding cross hybrid seedling the virgin phase shorten 4 to 5 years, greatly improve Kuerle delicious pear directive breeding efficiency, shorten the Kuerle delicious pear directive breeding cycle, it is with a wide range of applications.
The present invention is achieved through the following technical solutions:
The present invention sows then in conjunction with cross-fertilize seed and nurses young plants in hothouses, and carries out removing seed coat process and sprays GA vegetative period with gibberellin treatment, cross hybrid seedling3, cross hybrid seedling ring-type loses money instead of making money skin, cross hybrid seedling terminal bud grafting, dwarfing rootstock, sprays choline dichloride the multiple technologies measure integrated virgin phases shortening Kuerle delicious pear directive breeding cross hybrid seedling such as (CCC). The virgin phase of the cross hybrid seedling of Kuerle delicious pear directive breeding can be made 4 to 5 years are shortened by technical measures comprehensive above process, greatly improve Kuerle delicious pear directive breeding efficiency, shorten the Kuerle delicious pear directive breeding cycle, a large amount of manpowers, financial resources and material resources are saved, for the cultivation of Kuerle delicious pear directive breeding new variety opens new way, it provides novel method.
The present invention specifically provides the method shortening the Kuerle delicious pear directive breeding cross hybrid seedling virgin phase, is sowed the method nursed young plants in hothouses with hybrid breeding cultivation comprehensive integration then by employing Kuerle delicious pear cross-fertilize seed, and concrete method steps is as follows:
(1) harvesting of Kuerle delicious pear allocarpy: pluck ripe Kuerle delicious pear allocarpy between 6:00 AM 8, to be handled with care simultaneously, avoid mechanical injury.
(2) stripping of Kuerle delicious pear cross-fertilize seed and cleaning: Kuerle delicious pear allocarpy step (1) gathered refrigerates 3 days in freezer, and wherein the temperature of freezer is 4 DEG C; After refrigeration terminates, take out Kuerle delicious pear allocarpy, remove pulp and take out cenospecies, clear water rinsing cenospecies 58 minutes, and rub with emery cloth and frustrate, wash the viscous substance on cenospecies surface and the pulp of residual off, prepare Kuerle delicious pear cross-fertilize seed.
(3) remove the seed coat of Kuerle delicious pear cross-fertilize seed: Kuerle delicious pear cross-fertilize seed step (2) prepared is the clear water of 40 DEG C in temperature, invade bubble after 24 hours, with the seed coat of tweezers removal Kuerle delicious pear cross-fertilize seed gently.
(4) gibberellin treatment: the Kuerle delicious pear cross-fertilize seed of removal seed coat step (3) prepared invades bubble 12 hours in the Gibberellins solution of 0.35mg/L.
(5) Sand culture: the Kuerle delicious pear cross-fertilize seed taking out the process of step (4) Gibberellins solution, weight according to Kuerle delicious pear cross-fertilize seed: wet husky weight is the ratio of 1:5, Kuerle delicious pear cross-fertilize seed and wet sand are mixed, and it is encased in gauze, bundle is imbedded in the husky Tibetan container that wet sawdust is housed simultaneously, bundle covers the wet sawdust of 5cm again, then covers lid, sand is hidden container and puts into 4 DEG C of refrigerators, 30 days, husky Tibetan; Husky hide during once to be checked every 7 days, open bundle and stir 56 times, wet husky and seed is mixed, adds water, keep wet husky water content to be 55% 60%.
(6) sow: the Kuerle delicious pear cross-fertilize seed after step (5) being processed is seeded in the cave dish with substrate block.
(7) transplant: when the cross hybrid seedling in matrix nutrition alms bowl grows into 8 true leaves, being transplanted in greenhouse, the seeding row spacing of transplanting is 30cm × 50cm, meanwhile, in order to conveniently pour water and stop raw grass, adopt ridging Plastic-film corn.
(8) (patent can not adopt the non-technical of " management " printed words to belong to the elaboration of artificial interference)
Before transplanting cross hybrid seedling, in conjunction with arable land, the degree of depth in arable land is 40cm, applies base manure: fertilizer 2.5t/666.7cm2, chemical fertilizer: urea 20.0kg/666.7cm2, within 20 days, to pour water once, each irrigation quantity is 30m3/666.7cm2. Mode of pouring water easily adopts side to pour water, and namely pours water in side, ridge, avoids tree body backbone directly to contact bright water; For ensureing hybridization young plant fast-growth, transplant latter one month at cross hybrid seedling, in conjunction with pouring water, urea 5.0kg/666.7cm will be imposed2, every 20 days once; Transplant latter 6 months at cross hybrid seedling, within every 10 days, pour water once, quantitative change of pouring water: 60m3/666.7cm2, turn in conjunction with irrigation volume: urea 15.0kg/666.7cm2; Easily there is samping off in the early stage of cross hybrid seedling, the medicaments such as available derosal, m-tetrachlorophthalodinitrile, thiophanate methyl are alternately sprayed control, within every 15 days, spray once; The middle and later periods of cross hybrid seedling easily occurs pears aphid to cause harm, and affects the increment of cross hybrid seedling and the growth of the tender tip of cross hybrid seedling, it is possible to Avrmectin+pyridine worm miaow control, within every two weeks, spray once.
(9) regulation and control of greenhouse temperature: in order to ensure that cross hybrid seedling can carry out normal growth in winter, the temperature in greenhouse to be remained on more than 23 DEG C.
(10) field planting pear tree dwarfing rootstock: be graft hybridization seedling, the pear tree dwarfing rootstock in land in advance field planting life in 2 years: in short No. 1, the seeding row spacing of field planting is 100cm × 200cm.
(11) cross hybrid seedling sprays GA3: mid-May, cross hybrid seedling sprays GA3Promote cross hybrid seedling fast-growth, GA3The concentration of solution is 500ppm.
(12) cross hybrid seedling ring stripping: when cross hybrid seedling grows into 200cm, the method taking ring-type to lose money instead of making money skin at overhead 30cm place carries out ring stripping process, the width of ring stripping is the 1/10 of ring stripping place diameter.
(13) cross hybrid seedling terminal bud grafting: the terminal bud getting the cross hybrid seedling highly reaching more than 220cm, wherein the length of terminal bud is the bud in the 10cm of top, and grafting is on dwarfing rootstock.
(14) cross hybrid seedling spray on plants hormone choline dichloride (CCC) after terminal bud grafting, before mid-June, sprays choline dichloride (CCC) when the new tip of the terminal bud after to be grafted grows into 120cm, concentration is 1500ppm.
In the present invention, the water content that wet sawdust preferentially adopts is 50%, and the thickness that wet sawdust is hidden in container at sand is 5cm, it is intended that in order to keep the water content of sand and seed in the process of husky Tibetan.
In the present invention, described husky Tibetan container is preferentially adopted as wooden case container, and wooden case container is self-control wooden case with cover, and the length, width and height of wooden case are respectively 35cm, 25cm, 40cm. Wooden case good permeability, is beneficial to the breathing of cross-fertilize seed in the process of husky Tibetan, facilitates cross-fertilize seed to sprout.
The object of the present invention's ability substrate block is the seedling rate for improving Kuerle delicious pear cross-fertilize seed and transplanting survival rate.
In the present invention, the mode that ridging Plastic-film corn can adopt this area common, the ridging Plastic-film corn that the present invention preferentially adopts is: ridge height 15cm, wide 60cm, and the film covered is 70cm sealed polyethylene plastic.
In the present invention, the medicament method that alternately spraying control and Avrmectin+pyridine worm miaow control can adopt this area common and medicament, the present invention preferentially adopts the derosal to be: 50% carbendazol wettable powder 600 times, m-tetrachlorophthalodinitrile: 50% m-tetrachlorophthalodinitrile wettable powder 800 times; Thiophanate methyl is, 70% thiophanate methyl wettable powder 1000 times, and Avrmectin is, 2% abamectin emulsifiable concentrate 1500 times, and pyridine worm miaow is: 20% 1000 times, pyridine worm miaow pulvis.
The method of shortening Kuerle delicious pear directive breeding cross hybrid seedling provided by the invention virgin phase, the effect that Kuerle delicious pear cross hybrid seedling growth regulation can be yielded positive results for 3 years can be realized, result strain rate can reach 30%, and the cross hybrid seedling of traditional Kuerle delicious pear, need 7-8, the method of shortening Kuerle delicious pear directive breeding cross hybrid seedling provided by the invention virgin phase can realize within 3 years, seeing fruit, 4 years to 5 years are shortened than the virgin phase of traditional Kuerle delicious pear cross hybrid seedling, substantially reduce the virgin phase of cross hybrid seedling, shorten breeding process, shorten the result time, improve breeding efficiency, a large amount of field management costs and resource input can be saved simultaneously.
By implementing the concrete summary of the invention of the present invention, it is possible to reach following useful effect:
(1) method of the virgin phase of shortening Kuerle delicious pear directive breeding cross hybrid seedling provided by the invention, Kuerle delicious pear cross hybrid seedling growth regulation can be realized within 3 years, can yield positive results, result strain rate can reach 30%, and the cross hybrid seedling of traditional Kuerle delicious pear, need 7-8, by the 6 step measure process of the present invention, it is achieved within 3 years, see fruit, shorten 4 years to 5 years than the virgin phase of traditional Kuerle delicious pear cross hybrid seedling.
(2) method of the virgin phase of shortening Kuerle delicious pear directive breeding cross hybrid seedling provided by the invention, Kuerle delicious pear directive breeding cross hybrid seedling has been carried out 6 kinds of measure process, substantially reduce the virgin phase of Kuerle delicious pear directive breeding cross hybrid seedling, shorten breeding process, improve breeding efficiency, a large amount of field management costs and resource input can be saved simultaneously.
Embodiment
Below, lift example and the present invention is described, but, the present invention is not limited to following embodiment.
All raw and auxiliary materials, reagent and instrument, the equipment selected in the present invention are all selected for it is well known that, but do not limit the enforcement of the present invention, other reagent more well known in the art and all applicable enforcement implementing mode below the present invention of equipment, the % related in the present invention is weight percentage, except unless otherwise indicated.
Embodiment one: the method shortening the virgin phase of Kuerle delicious pear directive breeding cross hybrid seedling
The method of the virgin phase shortening Kuerle delicious pear directive breeding cross hybrid seedling carries out nursery and cultivation by the following method, and concrete method steps is as follows:
(1) Kuerle delicious pear cross-fertilize seed is sowed then and nurses young plants in hothouses
(1) harvesting of Kuerle delicious pear allocarpy: pluck the Kuerle delicious pear allocarpy that ripening degree is suitable between 6:00 AM 8, to be handled with care simultaneously, avoid mechanical injury.
(2) stripping of Kuerle delicious pear cross-fertilize seed and cleaning: Kuerle delicious pear allocarpy step (1) gathered refrigerates 3 days in freezer, and wherein the temperature of freezer is 4 DEG C; After refrigeration terminates, take out Kuerle delicious pear allocarpy, remove pulp and take out cenospecies, clear water rinsing cenospecies 58 minutes, and rub with emery cloth and frustrate, wash the viscous substance on cenospecies surface and the pulp of residual off, prepare Kuerle delicious pear cross-fertilize seed.
(3) remove the seed coat of Kuerle delicious pear cross-fertilize seed: Kuerle delicious pear cross-fertilize seed step (2) prepared is in the clear water of 40 DEG C in temperature, invade bubble 24 hours, then with the seed coat of tweezers removal Kuerle delicious pear cross-fertilize seed gently.
(4) gibberellin treatment: the Kuerle delicious pear cross-fertilize seed of removal seed coat step (3) prepared invades bubble 12 hours in the Gibberellins solution of 0.35mg/L.
(5) Sand culture: the Kuerle delicious pear cross-fertilize seed taking out the process of step (4) Gibberellins solution, weight according to Kuerle delicious pear cross-fertilize seed: wet husky weight is the ratio of 1:5, Kuerle delicious pear cross-fertilize seed and wet sand are mixed, and it is encased in gauze, bundle is imbedded in the husky Tibetan container that wet sawdust is housed simultaneously, bundle covers the wet sawdust of 5cm again, then covers lid, sand is hidden container and puts into 4 DEG C of refrigerators, 30 days, husky Tibetan.
(6) sow: the Kuerle delicious pear cross-fertilize seed after step (5) being processed is seeded in the cave dish with substrate block, it is the seedling rate for improving Kuerle delicious pear cross-fertilize seed and transplanting survival rate by the object of substrate block.
(7) transplant: when the cross hybrid seedling in matrix nutrition alms bowl grows into 8 true leaves, being transplanted in greenhouse, the seeding row spacing of transplanting is 30cm × 50cm, meanwhile, in order to conveniently pour water and stop raw grass, adopt ridging Plastic-film corn.
(2) management of Kuerle delicious pear cross hybrid seedling
(1) fertilizer of Kuerle delicious pear cross hybrid seedling, water and pest management:
Within 20 days, pouring water once, each irrigation quantity is 30m3/666.7cm2. Mode of pouring water easily adopts side to pour water, and namely pours water in side, ridge, avoids tree body backbone directly to contact bright water. For ensureing hybridization young plant fast-growth, transplant latter one month at cross hybrid seedling, in conjunction with pouring water, urea 5.0kg/666.7cm will be imposed2, every 20 days once; Transplant latter 6 months at cross hybrid seedling, within every 10 days, pour water once, quantitative change of pouring water: 60m3/666.7cm2, turn in conjunction with irrigation volume: urea 15.0kg/666.7cm2. Easily there is samping off in the early stage of cross hybrid seedling, the medicaments such as available derosal, m-tetrachlorophthalodinitrile, thiophanate methyl are alternately sprayed control, within every 15 days, spray once; The middle and later periods of cross hybrid seedling easily occurs pears aphid to cause harm, and affects the increment of cross hybrid seedling and the growth of the tender tip of cross hybrid seedling, it is possible to Avrmectin+pyridine worm miaow control, within every two weeks, spray once.
(2) regulation and control of greenhouse temperature: in order to ensure that cross hybrid seedling can carry out normal growth in winter, the temperature in greenhouse to be remained on more than 23 DEG C.
(3) field planting pear tree dwarfing rootstock: be graft hybridization seedling, the pear tree dwarfing rootstock in land in advance field planting life in 2 years: in short No. 1, the seeding row spacing of field planting is 100cm × 200cm.
(4) cross hybrid seedling sprays GA3: mid-May, cross hybrid seedling sprays GA3Promote cross hybrid seedling fast-growth, GA3The concentration of solution is 500ppm.
(5) cross hybrid seedling ring stripping: when cross hybrid seedling grows into 200cm, the method taking ring-type to lose money instead of making money skin at overhead 30cm place carries out ring stripping process, the width of ring stripping is one of 1/10 of ring stripping place diameter.
(6) cross hybrid seedling terminal bud grafting: the terminal bud getting the cross hybrid seedling highly reaching more than 220cm, wherein the length of terminal bud is the bud in the 10cm of top, and grafting is on dwarfing rootstock.
(7) cross hybrid seedling spray on plants hormone choline dichloride (CCC) after terminal bud grafting: before mid-June, spraying choline dichloride (CCC) when the new tip of the terminal bud after to be grafted grows into 120cm, concentration is 1500ppm.
In the present invention, the water content that wet sawdust preferentially adopts is 50%, and the thickness that wet sawdust is hidden in container at sand is 5cm, it is intended that in order to keep the water content of sand and seed in the process of husky Tibetan.
In the present invention, described husky Tibetan container is preferentially adopted as wooden case container, and wooden case container is self-control wooden case with cover, and the length, width and height of wooden case are respectively 35cm, 25cm, 40cm. Wooden case good permeability, is beneficial to the breathing of cross-fertilize seed in the process of husky Tibetan, facilitates cross-fertilize seed to sprout.
The object of the present invention's ability substrate block is the seedling rate for improving Kuerle delicious pear cross-fertilize seed and transplanting survival rate.
In the present invention, the mode that ridging Plastic-film corn can adopt this area common, the ridging Plastic-film corn that the present invention preferentially adopts is: ridge height 15cm, wide 60cm, and the film covered is 70cm sealed polyethylene plastic.
In the present invention, the medicament method that alternately spraying control and Avrmectin+pyridine worm miaow control can adopt this area common and medicament, the present invention preferentially adopts the derosal to be: 50% carbendazol wettable powder 600 times, m-tetrachlorophthalodinitrile: 50% m-tetrachlorophthalodinitrile wettable powder 800 times; Thiophanate methyl is, 70% thiophanate methyl wettable powder 1000 times, and Avrmectin is, 2% abamectin emulsifiable concentrate 1500 times, and pyridine worm miaow is: 20% 1000 times, pyridine worm miaow pulvis.
The method of shortening Kuerle delicious pear directive breeding cross hybrid seedling provided by the invention virgin phase, the effect that Kuerle delicious pear cross hybrid seedling growth regulation can be yielded positive results for 3 years can be realized, result strain rate can reach 30%, and the cross hybrid seedling of traditional Kuerle delicious pear, need 7-8, the method of the virgin phase of shortening Kuerle delicious pear directive breeding cross hybrid seedling provided by the invention can realize within 3 years, seeing fruit, 4 years to 5 years are shortened than the virgin phase of traditional Kuerle delicious pear cross hybrid seedling, substantially reduce the virgin phase of cross hybrid seedling, shorten breeding process, shorten the result time, improve breeding efficiency, a large amount of field management costs and resource input can be saved simultaneously.
Embodiment two: the method shortening the virgin phase nursery of Kuerle delicious pear directive breeding cross hybrid seedling
Ripe Kuerle delicious pear allocarpy is plucked between 6:00 AM 8, freezer refrigerates 3 days, after refrigeration terminates, take out cenospecies, clear water rinsing cenospecies 58 minutes, wash the viscous substance on cenospecies surface and the pulp of residual off, obtain Kuerle delicious pear cross-fertilize seed, clean water is invaded bubble 24 hours, with the seed coat of tweezers removal Kuerle delicious pear cross-fertilize seed gently, the Kuerle delicious pear cross-fertilize seed removing seed coat invades bubble 12 hours in the Gibberellins solution of 0.35mg/L, weight according to Kuerle delicious pear cross-fertilize seed: wet husky weight is the ratio of 1:5, Kuerle delicious pear cross-fertilize seed and wet sand are mixed, and it is encased in gauze, bundle is imbedded simultaneously and it is equipped with in wet husky husky Tibetan container, sand is hidden container and puts into 4 DEG C of refrigerators, 30 days, husky Tibetan.Being seeded into by Kuerle delicious pear cross-fertilize seed in the dish of substrate block cave, when the cross hybrid seedling of substrate block grows into 8 true leaves, be transplanted in greenhouse, the seeding row spacing of transplanting is 30cm × 50cm, meanwhile, in order to conveniently pour water and stop raw grass, adopts ridging Plastic-film corn.
Embodiment three: the method shortening the virgin period management of Kuerle delicious pear directive breeding cross hybrid seedling
The rich water quality management of Kuerle delicious pear cross hybrid seedling: pour water once in 20 days, each irrigation quantity is 30m3/666.7cm2. Mode of pouring water easily adopts side to pour water, and namely pours water in side, ridge, avoids tree body backbone directly to contact bright water. For ensureing hybridization young plant fast-growth, transplant latter one month at cross hybrid seedling, in conjunction with pouring water, urea 5.0kg/666.7cm will be imposed2, every 20 days once; Transplant latter 6 months at cross hybrid seedling, within every 10 days, pour water once, quantitative change of pouring water: 60m3/666.7cm2, turn in conjunction with irrigation volume: urea 15.0kg/666.7cm2. Easily there is samping off in the early stage of cross hybrid seedling, the medicaments such as available derosal, m-tetrachlorophthalodinitrile, thiophanate methyl are alternately sprayed control, within every 15 days, spray once; The middle and later periods of cross hybrid seedling easily occurs pears aphid to cause harm, and affects the increment of cross hybrid seedling and the growth of the tender tip of cross hybrid seedling, it is possible to Avrmectin+pyridine worm miaow control, within every two weeks, spray once. Employing derosal is: 50% carbendazol wettable powder 600 times, m-tetrachlorophthalodinitrile: 50% m-tetrachlorophthalodinitrile wettable powder 800 times, thiophanate methyl: 70% thiophanate methyl wettable powder 1000 times. Avrmectin: 2% abamectin emulsifiable concentrate 1500 times, pyridine worm miaow is: 20% 1000 times, pyridine worm miaow pulvis. The temperature in greenhouse to be remained on more than 23 DEG C, is graft hybridization seedling, the pear tree dwarfing rootstock in land in advance field planting life in 2 years: in short No. 1, the seeding row spacing of field planting is 100cm × 200cm, mid-May, sprays GA on cross hybrid seedling3Promote cross hybrid seedling fast-growth, GA3The concentration of solution is 500ppm, when cross hybrid seedling grows into 200cm, the method taking ring-type to lose money instead of making money skin at overhead 30cm place carries out ring stripping process, the width of ring stripping is one of 1/10 of ring stripping place diameter, gets the terminal bud of the cross hybrid seedling highly reaching more than 220cm, and wherein the length of terminal bud is the bud in the 10cm of top, grafting is on dwarfing rootstock, before mid-June, spraying choline dichloride (CCC) when the new tip of the terminal bud after to be grafted grows into 120cm, concentration is 1500ppm.
The present invention adopt the cross-fertilize seed of above-mentioned offer sow then nurse young plants in hothouses, cross hybrid seedling spray GA vegetative period3, cross hybrid seedling ring-type lose money instead of making money skin, dwarfing rootstock, cross hybrid seedling terminal bud grafting, spray 6 kinds of measures such as ethrel, reach and shorten the virgin phase, cost-saving, it is to increase the object of breeding efficiency.
Embodiment four: the Measures compare of the present invention and tradition Kuerle delicious pear directive breeding
The method of tradition Kuerle delicious pear directive breeding, after hybridization pollination, cross hybrid seedling is cultivated in sowing in the 2nd year, allocarpy is placed on shady and cool place after gathering, until fruit by ripe rotten time take out cenospecies, cross-fertilize seed is dried in the shade after taking out, it is placed on shady and cool place and stores 3 months, when soaking 24 with clear water after 3 months hour, do not remove seed coat, direct sand is hidden, during husky Tibetan, the ratio of sand and seed is 3:1, the husky Tibetan time is 2 to 3 months, Sha Zanghou is directly seeded into the big Tanaka in breeding garden, until cross hybrid seedling is yielded positive results, sowing time is early April, the mode of sowing is that trench digging is live, not ridging during sowing, the seeding row spacing of sowing is 50cm*400cm, timely weeding and pouring water after emerging, weeding 3 times/month, pour water based on broad irrigation, pour water 2 time/month, fertilising is based on base manure, topdress 1 time/month, after cross hybrid seedling grows into 4 years, in time the branch of below trunk 120cm is all removed, and the branch of more than trunk 120cm is carried out short section and the shaping pruning modes such as remove, treat that cross hybrid seedling grows into the 6th year, start the branch to cross hybrid seedling and carry out pinching, turn round slightly, draw the process such as branch, combine control water control fertilizer simultaneously and wait process, promote the bud differentiation of cross hybrid seedling and become flower, after process, cross hybrid seedling can be yielded positive results after growing into the 7th year like this, specifically in table 1..
Table 1: with the contrast table of tradition Kuerle delicious pear directive breeding condition
The method of table 2 the present invention and the contrast of traditional method
Illustrate that the present invention shortens the method for the virgin phase of Kuerle delicious pear directive breeding cross hybrid seedling by table 2 different from the method for the virgin phase of traditional method shortening Kuerle delicious pear directive breeding cross hybrid seedling, adopt the method for shortening Kuerle delicious pear directive breeding cross hybrid seedling provided by the invention virgin phase, achieve the effect that Kuerle delicious pear cross hybrid seedling growth regulation can be yielded positive results for 3 years, result strain rate can reach 30%, and the cross hybrid seedling of traditional Kuerle delicious pear, need 7-8, the method of shortening Kuerle delicious pear directive breeding cross hybrid seedling provided by the invention virgin phase can realize within 3 years, seeing fruit, 4 years to 5 years are shortened than the virgin phase of traditional Kuerle delicious pear cross hybrid seedling, substantially reduce the virgin phase of cross hybrid seedling, shorten breeding process, shorten the result time, improve breeding efficiency, a large amount of field management costs and resource input can be saved simultaneously.
As mentioned above; the present invention can be realized preferably; the above embodiments are only the preferred embodiment of the present invention be described; not the scope of the present invention is limited; under the prerequisite not departing from inventive design spirit; the various distortion that the technical scheme of the present invention is made by those of ordinary skill in the art and improvement, all should fall in the protection domain that the present invention determines.
Claims (9)
1. shortening the method for Kuerle delicious pear directive breeding cross hybrid seedling virgin phase, sowed the method nursed young plants in hothouses with hybrid breeding cultivation comprehensive integration by employing Kuerle delicious pear cross-fertilize seed then, concrete method steps is as follows:
(1) harvesting of Kuerle delicious pear allocarpy: pluck ripe Kuerle delicious pear allocarpy between 6:00 AM 8, to be handled with care simultaneously, avoid mechanical injury;
(2) stripping of Kuerle delicious pear cross-fertilize seed and cleaning: Kuerle delicious pear allocarpy step (1) gathered refrigerates 3 days in freezer, and wherein the temperature of freezer is 4 DEG C; After refrigeration terminates, take out Kuerle delicious pear allocarpy, remove pulp and take out cenospecies, clear water rinsing cenospecies 58 minutes, and rub with emery cloth and frustrate, wash the viscous substance on cenospecies surface and the pulp of residual off, prepare Kuerle delicious pear cross-fertilize seed;
(3) remove the seed coat of Kuerle delicious pear cross-fertilize seed: Kuerle delicious pear cross-fertilize seed step (2) prepared is the clear water of 40 DEG C in temperature, invade bubble after 24 hours, with the seed coat of tweezers removal Kuerle delicious pear cross-fertilize seed gently;
(4) gibberellin treatment: the Kuerle delicious pear cross-fertilize seed of removal seed coat step (3) prepared invades bubble 12 hours in the Gibberellins solution of 0.35mg/L;
(5) Sand culture: the Kuerle delicious pear cross-fertilize seed taking out the process of step (4) Gibberellins solution, weight according to Kuerle delicious pear cross-fertilize seed: wet husky weight is the ratio of 1:5, Kuerle delicious pear cross-fertilize seed and wet sand are mixed, and it is encased in gauze, bundle is imbedded in the husky Tibetan container that wet sawdust is housed simultaneously, bundle covers the wet sawdust of 5cm again, then covers lid, sand is hidden container and puts into 4 DEG C of refrigerators, 30 days, husky Tibetan; Husky hide during once to be checked every 7 days, open bundle and stir 56 times, wet husky and seed is mixed, adds water, keep wet husky water content to be 55% 60%;
(6) sow: the Kuerle delicious pear cross-fertilize seed after step (5) being processed is seeded in the cave dish with substrate block;
(7) transplant: when the cross hybrid seedling in matrix nutrition alms bowl grows into 8 true leaves, being transplanted in greenhouse, the seeding row spacing of transplanting is 30cm × 50cm, adopt ridging Plastic-film corn;
(8) before transplanting cross hybrid seedling, in conjunction with arable land, the degree of depth in arable land is 40cm, applies base manure: fertilizer 2.5t/666.7cm2, chemical fertilizer: urea 20.0kg/666.7cm2, within 20 days, to pour water once, each irrigation quantity is 30m3/666.7cm2; Mode of pouring water easily adopts side to pour water, and namely pours water in side, ridge, avoids tree body backbone directly to contact bright water; For ensureing hybridization young plant fast-growth, transplant latter one month at cross hybrid seedling, in conjunction with pouring water, urea 5.0kg/666.7cm will be imposed2, every 20 days once; Transplant latter 6 months at cross hybrid seedling, within every 10 days, pour water once, quantitative change of pouring water: 60m3/666.7cm2, turn in conjunction with irrigation volume: urea 15.0kg/666.7cm2; Easily there is samping off in the early stage of cross hybrid seedling, adopts the medicament such as derosal, m-tetrachlorophthalodinitrile, thiophanate methyl alternately to spray control, within every 15 days, spray once; The middle and later periods of cross hybrid seedling easily occurs pears aphid to cause harm, and affects the increment of cross hybrid seedling and the growth of the tender tip of cross hybrid seedling, it is possible to Avrmectin+pyridine worm miaow control, within every two weeks, spray once;
(9) regulation and control of greenhouse temperature: in order to ensure that cross hybrid seedling can carry out normal growth in winter, the temperature in greenhouse to be remained on more than 23 DEG C;
(10) field planting pear tree dwarfing rootstock: be graft hybridization seedling, the pear tree dwarfing rootstock in land in advance field planting life in 2 years: in short No. 1, the seeding row spacing of field planting is 100cm × 200cm;
(11) cross hybrid seedling sprays GA3: mid-May, cross hybrid seedling sprays GA3Promote cross hybrid seedling fast-growth, GA3The concentration of solution is 500ppm;
(12) cross hybrid seedling ring stripping: when cross hybrid seedling grows into 200cm, the method taking ring-type to lose money instead of making money skin at overhead 30cm place carries out ring stripping process, the width of ring stripping is the 1/10 of ring stripping place diameter;
(13) cross hybrid seedling terminal bud grafting: the terminal bud getting the cross hybrid seedling highly reaching more than 220cm, wherein the length of terminal bud is the bud in the 10cm of top, and grafting is on dwarfing rootstock;
(14) cross hybrid seedling spray on plants hormone choline dichloride (CCC) after terminal bud grafting, before mid-June, sprays choline dichloride (CCC) when the new tip of the terminal bud after to be grafted grows into 120cm, concentration is 1500ppm.
2. the method shortening the Kuerle delicious pear directive breeding cross hybrid seedling virgin phase as claimed in claim 1, it is characterised in that, the water content that described wet sawdust adopts is 50%, and the thickness that wet sawdust is hidden in container at sand is 5cm.
3. the method shortening the Kuerle delicious pear directive breeding cross hybrid seedling virgin phase as claimed in claim 1, it is characterised in that, described husky Tibetan container is adopted as wooden case container, and wooden case container is self-control wooden case with cover, and the length, width and height of wooden case are respectively 35cm, 25cm, 40cm.
4. the method shortening the Kuerle delicious pear directive breeding cross hybrid seedling virgin phase as claimed in claim 1, it is characterized in that, the ridging Plastic-film corn that described ridging Plastic-film corn adopts is: ridge height 15cm, wide 60cm, the film covered is 70cm sealed polyethylene plastic.
5. the method shortening the Kuerle delicious pear directive breeding cross hybrid seedling virgin phase as claimed in claim 1, it is characterised in that, described employing derosal is: 50% carbendazol wettable powder 600 times.
6. the method shortening the Kuerle delicious pear directive breeding cross hybrid seedling virgin phase as claimed in claim 1, it is characterised in that, described employing m-tetrachlorophthalodinitrile: 50% m-tetrachlorophthalodinitrile wettable powder 800 times.
7. the method shortening the Kuerle delicious pear directive breeding cross hybrid seedling virgin phase as claimed in claim 1, it is characterised in that, described employing thiophanate methyl is, 70% thiophanate methyl wettable powder 1000 times.
8. the method shortening the Kuerle delicious pear directive breeding cross hybrid seedling virgin phase as claimed in claim 1, it is characterised in that, described employing Avrmectin is, 2% abamectin emulsifiable concentrate 1500 times.
9. the method shortening the Kuerle delicious pear directive breeding cross hybrid seedling virgin phase as claimed in claim 1, it is characterised in that, described employing pyridine worm miaow is: 20% 1000 times, pyridine worm miaow pulvis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610061391.7A CN105660237B (en) | 2016-01-29 | 2016-01-29 | A method of shortening Kuerle delicious pear directive breeding cross hybrid seedling juvenile phase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610061391.7A CN105660237B (en) | 2016-01-29 | 2016-01-29 | A method of shortening Kuerle delicious pear directive breeding cross hybrid seedling juvenile phase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105660237A true CN105660237A (en) | 2016-06-15 |
| CN105660237B CN105660237B (en) | 2018-08-17 |
Family
ID=56303019
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610061391.7A Expired - Fee Related CN105660237B (en) | 2016-01-29 | 2016-01-29 | A method of shortening Kuerle delicious pear directive breeding cross hybrid seedling juvenile phase |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105660237B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106665207A (en) * | 2016-12-22 | 2017-05-17 | 宣威市龙津生物科技有限责任公司 | Method for preventing damping-off in seedling stage of herba erigerontis float breeding |
| CN107258435A (en) * | 2017-06-27 | 2017-10-20 | 江西省农业科学院园艺研究所 | It is a kind of to promote citrus to do sth. in advance the method yielded positive results |
| CN107466766A (en) * | 2017-08-18 | 2017-12-15 | 新疆生产建设兵团第二师农业科学研究所 | The mating system of Kuerle delicious pear crossbreeding nursery stock |
| CN108308008A (en) * | 2018-02-11 | 2018-07-24 | 沈阳农业大学 | A kind of Ussurian pear seedlings mating system |
| CN109156359A (en) * | 2018-10-24 | 2019-01-08 | 安徽农业大学 | A kind of construction method of the direct organogenesis approach of Dangshan pear |
| CN110214616A (en) * | 2019-06-03 | 2019-09-10 | 贵阳市农业试验中心 | A kind of breeding method that loquat hybrid new breed is trained fastly |
| CN111492883A (en) * | 2020-05-07 | 2020-08-07 | 山西省农业科学院果树研究所 | Efficient apple crossbreeding method |
| CN115024143A (en) * | 2021-10-29 | 2022-09-09 | 浙江省农业科学院 | Method for promoting loquat hybrid seeds to sprout and grow seedlings quickly |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0998671A (en) * | 1995-10-04 | 1997-04-15 | Dainippon Printing Co Ltd | Biodegradable raising seedling pot |
| CN101507383A (en) * | 2009-04-03 | 2009-08-19 | 湖北省农业科学院果树茶叶研究所 | Germinating breeding method of Chinese pear cross-breed |
| CN101884294A (en) * | 2010-06-25 | 2010-11-17 | 西南大学柑桔研究所 | Method for reducing juvenile period of hybrid tangerine |
| CN101982057A (en) * | 2010-08-18 | 2011-03-02 | 南京农业大学 | Method for boosting pear hybrid seedlings to form flowers ahead of time |
| CN102217484A (en) * | 2011-05-19 | 2011-10-19 | 湖北省农业科学院果树茶叶研究所 | Easy and fast seedling method for hybrid pear seeds |
| CN102783413A (en) * | 2012-08-30 | 2012-11-21 | 江苏省农业科学院 | Fast crossbreeding method of pear tree |
| CN103392550A (en) * | 2013-07-25 | 2013-11-20 | 湖北省农业科学院果树茶叶研究所 | Method of increasing seedling rate and seedling quality for sand pear hybrid seeds |
| CN104365214A (en) * | 2014-11-03 | 2015-02-25 | 安徽省农业科学院园艺研究所 | Simple method for improving emergence rate of pear hybrid seeds |
| CN104938286A (en) * | 2015-07-07 | 2015-09-30 | 江苏省农业科学院 | Hybridizing, sowing and seedling raising method of pears |
-
2016
- 2016-01-29 CN CN201610061391.7A patent/CN105660237B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0998671A (en) * | 1995-10-04 | 1997-04-15 | Dainippon Printing Co Ltd | Biodegradable raising seedling pot |
| CN101507383A (en) * | 2009-04-03 | 2009-08-19 | 湖北省农业科学院果树茶叶研究所 | Germinating breeding method of Chinese pear cross-breed |
| CN101884294A (en) * | 2010-06-25 | 2010-11-17 | 西南大学柑桔研究所 | Method for reducing juvenile period of hybrid tangerine |
| CN101982057A (en) * | 2010-08-18 | 2011-03-02 | 南京农业大学 | Method for boosting pear hybrid seedlings to form flowers ahead of time |
| CN102217484A (en) * | 2011-05-19 | 2011-10-19 | 湖北省农业科学院果树茶叶研究所 | Easy and fast seedling method for hybrid pear seeds |
| CN102783413A (en) * | 2012-08-30 | 2012-11-21 | 江苏省农业科学院 | Fast crossbreeding method of pear tree |
| CN103392550A (en) * | 2013-07-25 | 2013-11-20 | 湖北省农业科学院果树茶叶研究所 | Method of increasing seedling rate and seedling quality for sand pear hybrid seeds |
| CN104365214A (en) * | 2014-11-03 | 2015-02-25 | 安徽省农业科学院园艺研究所 | Simple method for improving emergence rate of pear hybrid seeds |
| CN104938286A (en) * | 2015-07-07 | 2015-09-30 | 江苏省农业科学院 | Hybridizing, sowing and seedling raising method of pears |
Non-Patent Citations (2)
| Title |
|---|
| 凌天亚等: "主干环割对梨杂种后代童期、童程及相关指标的影响", 《中国果树》 * |
| 崔艳波等: "《梨杂交后代童期和童程的研究》", 《中国农学通报》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106665207A (en) * | 2016-12-22 | 2017-05-17 | 宣威市龙津生物科技有限责任公司 | Method for preventing damping-off in seedling stage of herba erigerontis float breeding |
| CN106665207B (en) * | 2016-12-22 | 2019-12-27 | 宣威市龙津生物科技有限责任公司 | Method for preventing occurrence of cataplexy of fleabane flower in floating seedling culture seedling stage |
| CN107258435A (en) * | 2017-06-27 | 2017-10-20 | 江西省农业科学院园艺研究所 | It is a kind of to promote citrus to do sth. in advance the method yielded positive results |
| CN107466766A (en) * | 2017-08-18 | 2017-12-15 | 新疆生产建设兵团第二师农业科学研究所 | The mating system of Kuerle delicious pear crossbreeding nursery stock |
| CN108308008A (en) * | 2018-02-11 | 2018-07-24 | 沈阳农业大学 | A kind of Ussurian pear seedlings mating system |
| CN109156359A (en) * | 2018-10-24 | 2019-01-08 | 安徽农业大学 | A kind of construction method of the direct organogenesis approach of Dangshan pear |
| CN110214616A (en) * | 2019-06-03 | 2019-09-10 | 贵阳市农业试验中心 | A kind of breeding method that loquat hybrid new breed is trained fastly |
| CN110214616B (en) * | 2019-06-03 | 2021-07-27 | 贵阳市农业试验中心 | Rapid cultivation breeding method for new loquat hybrid variety |
| CN111492883A (en) * | 2020-05-07 | 2020-08-07 | 山西省农业科学院果树研究所 | Efficient apple crossbreeding method |
| CN115024143A (en) * | 2021-10-29 | 2022-09-09 | 浙江省农业科学院 | Method for promoting loquat hybrid seeds to sprout and grow seedlings quickly |
| CN115024143B (en) * | 2021-10-29 | 2024-03-26 | 浙江省农业科学院 | Method for promoting loquat hybrid seeds to sprout and quickly grow seedlings |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105660237B (en) | 2018-08-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105660237B (en) | A method of shortening Kuerle delicious pear directive breeding cross hybrid seedling juvenile phase | |
| CN105028110B (en) | A kind of large cherry control root downgrades dense planting cultural method high | |
| CN101637092B (en) | Method for culturing large eucalyptus seedlings | |
| CN108476843A (en) | A kind of cultural method that August is fried | |
| CN104620828B (en) | A kind of wild Fructus Choerospondiatis becomes the method that artificial culture becomes Landscape Trees | |
| CN106069458B (en) | A kind of method for culturing seedlings of shaddock | |
| CN104186171A (en) | Pepper dwarf thick planting pick-free high-yield planting method | |
| CN108668731A (en) | A kind of breeding method of oriental cherry grafting | |
| CN106258431A (en) | A kind of black Fructus Lycii field seeding method for culturing seedlings being suitable for arid area | |
| CN107896863A (en) | A kind of breeding method of capsicum | |
| CN109168885A (en) | A kind of navel orange cultivating superior high-yield method | |
| CN101773046B (en) | Groundcherry seedling propagation method | |
| CN1918971B (en) | Seeds production method for few-seed purple eggplant first-filial generation | |
| CN103798054A (en) | High-yield high-efficiency cultivation mode for interplanting good mulberry with konjac | |
| CN106034876A (en) | High-yield plantation method for paddy rice | |
| CN106613659B (en) | Grafting cultivation method of euonymus plant golden beetle | |
| CN105766302B (en) | A kind of organic brassica campestris var purpurea exempts from pesticide insect prevention implantation methods | |
| CN109526432B (en) | Sweet cherry seedling raising and garden building method in high-altitude cold areas | |
| CN107409684A (en) | A kind of greenhouse sponge gourd implantation methods for improving yield | |
| CN105075644B (en) | A kind of breeding method of spring flower cabbage, summer Lettuce and autumn youth-and-old-age | |
| CN109496655B (en) | Seed germination and seedling raising method for camellia azalea | |
| CN106106192A (en) | A kind of method for building up of Garbo fruit tissue culturing system | |
| CN106561385A (en) | Morinda citrifolia propagation method applicable to inland hot zone | |
| CN105325251B (en) | A kind of live quick-growing cultivation method of Jatropha curcas | |
| CN108901575A (en) | A kind of three step cultivating methods improving cherry hybrid planting percent and precocity fruiting |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180817 Termination date: 20220129 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |