CN106973581A - A kind of method for releasing pears seed dormancy - Google Patents
A kind of method for releasing pears seed dormancy Download PDFInfo
- Publication number
- CN106973581A CN106973581A CN201710224699.3A CN201710224699A CN106973581A CN 106973581 A CN106973581 A CN 106973581A CN 201710224699 A CN201710224699 A CN 201710224699A CN 106973581 A CN106973581 A CN 106973581A
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- seed
- water
- absorbent cotton
- dormancy
- culture dish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of method for releasing seed dormancy.It is related to Seed imbibition, shows money or valuables one carries unintentionally and rudiment, belongs to plant physiology and production cultivation field.The present invention soaks seed by early stage, is then wrapped in absorbent cotton on kind of skin, using absorbent cotton performance of keeping humidity is good and the characteristics of good permeability, then thoroughly breaks by cool stratification pears seed dormancy.This method has the advantages that easily operated, step is simple, is easy to observation, and the effect of breaking dormancy is good, and is wrapped up with absorbent cotton and convenient add chemical agent and hormone to study them to releasing effects of seed dormancy.
Description
Technical field
The present invention relates to a kind of method for releasing pears seed dormancy, it is related to Seed imbibition, shows money or valuables one carries unintentionally and rudiment, belongs to plant life
Of science and production cultivation field.
Background technology
Pear rootstock seed has hibernation feature, in production, often seed is carried out the cold stratification of some months to beat
Broken dormancy.But the temperature and time of Stratificated treatment needs that sowing time has been determined in advance, and otherwise can not broken because of premature germination or
Optimal seedtime is missed except dormancy, this brings difficulty to the upper large area plantation of production and nursery.Therefore seed dormancy is broken
To shortening breeding cycle, improving nursery rate, improve seedling quality and reduce production cost significant.
The stock of pears seedling and the seed of filial generation are because by pericarp obstacle and inhibiting substances, plant embryo dormancy and embryo
The influence of the reasons such as after-ripening, it is impossible to as other seeds, such as：Cucumber, wheat, paddy rice etc. are equally sprouted, it is necessary to using breaking dormancy
Method handled and just can normally be sprouted.In conventional breeding and cultivation practices, have to carry out low temperature after collection seed
Stratificated treatment, Second Year sowing is that is, time-consuming to take a lot of work, and time length of the seed because of cold stratification during sand storage is held
Or often occur to rot during lamination, the normal breaking dormancy of seed can be influenceed, causes that after planting seed can not be sprouted or germination percentage is low,
Cause damage.
The content of the invention
It is an object of the invention to provide a kind of method for releasing seed dormancy, it can be easy to understand and grasp pears seed dormancy rule
And observe the change of seed during breaking dormancy.
The present invention carries out control and holding that earthing of seeds difficult point is humidity in culture dish, and absorbent cotton has good guarantor
It is wet effect and aeration is good.The use of absorbent cotton is the innovative point in this lamination method in this discovery.
Realizing the technical solution of foregoing invention purpose is：
A kind of method for releasing seed dormancy, comprises the following steps：
1) seed early stage is handled
Seed is selected with wet cleaning process first, full grains, seed of the same size are selected.(specific method is that hand grabs one
Seed is put into the beaker for filling water, selects full grains, seed of the same size) secondly with 2%NaClO to seed disinfection,
Bacterial invasion is prevented, the seed after sterilization is cleaned 1-3 times with water.Finally seed is put into the beaker for fill water and soaks 24h, allowed
Seed fully absorbs water.
2) lamination seed
Seed is wrapped up with absorbent cotton, absorbent cotton is torn into sheet, it is as far as possible thin, it is wrapped in seed coat surface.The kind wrapped
Son is put between culture dish, seed and leaves certain distance, with liquid-transfering gun water suction moistening absorbent cotton.Then culture dish is put into box
Or carry out shading treatment with masking foil parcel.Eventually it is put into 4 DEG C of refrigerator laminations.Showed money or valuables one carries unintentionally with seed and terminate lamination for signal.
3) humidity is controlled
During earthing of seeds, the control of humidity is particularly important.A water was poured every 3-4 days.Only needed when watering by
Absorbent cotton is soaked, and ponding can not be had by having poured in the water around water liquid-transfering gun absorption seed, culture dish, prevent seed from sending out
It is mould.
4) rudiment is tested
Bud bed is first carried out during earthing of seeds, two layers of filter paper need to be only padded in culture dish and is moistened.After lamination terminates
The absorbent cotton of seed coat surface is removed, seed is cleaned and is put into ready bud bed.25 DEG C of constant temperature are put into masking foil parcel to do
In dry case, rudiment test is carried out in dark conditions.A germination percentage is counted daily and moisturizing of watering (is changed once for 2-3 days
Filter paper).
Absorbent cotton has the ability and aeration of moisturizing good, can solve and how to keep during earthing of seeds
The difficult point of seed overly moist or overdrying.The innovative point of the present invention is that intrinsic effect absorbent cotton and molecule experiments are combined
Together, the problem of this not only solves humidity during earthing of seeds and for the processing of follow-up experiment reagent, observes, grinds
Study carefully physiological change of the seed during breaking dormancy significant.
Pears seed by the reason such as pericarp obstacle and inhibiting substances, kind embryo dormancy and kind embryo after-ripening because influenceed, it is impossible to
As other seeds, such as：Cucumber, wheat, paddy rice etc. equally sprout, it is necessary to can normally be sprouted by the method for breaking dormancy.Planting
A series of physiological change can occur during sub- breaking dormancy, in order to which the physiological change studied during this will necessarily be right
Seed during this carries out treatment research, and the seed wrapped up in culture dish using absorbent cotton easily carries out reagent to it
Processing, such as：It can solve after different time sections carry out HORMONE TREATMENT to seed, seed RNA change etc.；Degreasing in the present invention
It is huge that cotton acts on during earthing of seeds, is that follow-up study lays the foundation, therefore this invention is significant.
Beneficial effects of the present invention:
1) break the method for pears seed dormancy using culture dish Stratificated treatment, whole can observe kind during lamination
The change that son occurs, preferably studies its Dormancy Characteristics and the method for breaking dormancy；
2) breaking seed dormancy has many methods：Mechanical treatment, cool stratification and Chemical treatment, and use culture dish
Culture bed lamination effect but also conveniently addition chemical agent and the hormone of can not only carrying out is done to study them to releasing seed to stop
The effect of dormancy；
3) present invention has solid theoretical foundation, and several conditions (low temperature, ventilation and humidity) of cool stratification vernalization are all
Possess, method is easier, it is easy to operation and practical application.
Brief description of the drawings
Fig. 1 is the method for the invention culture dish lamination flow chart；
Fig. 2 is culture dish lamination pictorial diagram of the present invention；
Fig. 3 is seed germination pictorial diagram of the present invention.
Embodiment
Below in conjunction with the accompanying drawings and with specific embodiment, the present invention is furture elucidated.It should be understood that these embodiments are only used for
The bright present invention rather than limitation the scope of the present invention, after the present invention has been read, those skilled in the art are to the present invention's
The modification of the various equivalent form of values falls within the application appended claims limited range.
Fruit Tree Seeds dormancy is very common phenomenon, and complicated the reason for dormancy.Often due to Fruit Tree Seeds in production
Dormancy fails timely releasing, and the phenomenon for causing germination percentage low or even next year germinateing has had a strong impact on normal nursery work.Therefore,
The method for exploring releasing seed dormancy is significant in production.The method of our the application present invention, can be easy, effective
Seed dormancy is released, is that research seed dormancy rule and releasing seed dormancy lay the foundation.
Following examples, are only best understood from the present invention by way of example, are not limiting the scope of the invention.
Embodiment 1
This example is using bean pear seed as experiment material, and process is as follows：
1st, seed wet cleaning process is selected.Hand is grabbed a seed and is put into the beaker for filling water, and it is shrivelled that removing is kept afloat
Seed, selects full grains, and then unabroken seed is placed on filter paper and spontaneously dries.
2nd, seed disinfection prevents bacterial invasion with 2%NaClO to seed disinfection, and the seed after sterilization cleans 1-3 with water
It is secondary.
3rd, the seed after sterilization is put into the beaker for fill water by immersion seed soaks 24h, allows seed to suction moisture.
4th, wrap up the good absorbent cotton of seed preparation, by absorbent cotton tear it is as far as possible thinner, blocked up seed is easily mouldy, take off
Fat cotton is wrapped in seed coat surface, is put into culture dish.
5th, the water suction of absorbent cotton liquid-transfering gun is soaked to soak after absorbent cotton, absorbent cotton moistening with liquid-transfering gun washout absorbent cotton week
Enclose moisture.There can not be ponding in culture dish, prevent the mold of seed.
6th, Stratification condition lamination need to be carried out in 4 DEG C of refrigerators, and must carry out shading treatment to culture dish, will can be trained
Foster ware is put into box or superscribed masking foil.A water was poured every 3-4 days, absorbent cotton need to only be moistened.
7th, the different Pear varieties seed dormancy degree of Stratification time are different, showed money or valuables one carries unintentionally with seed and terminate lamination for signal, about
Need 40-50 days.
8th, culture dish of the bud bed from 9cm or 12cm is made, the inside pad two layers of filter paper is simultaneously moistened.
9th, the good seed of lamination is removed absorbent cotton and cleaned by rudiment test, is put into bud bed.By culture dish masking foil
Parcel is put into 25 DEG C of constant incubators, and rudiment test is carried out in dark conditions.Keeping filter paper constant moisture, (2-3 days more
Change a filter paper) and daily count a germination percentage.
In experimental study, according to research contents can carry out different disposal to seed and the sand lamination of routine it is inconvenient to
Seed addition medicine processing；Therefore it is found that and is carried out in the medium with absorbent cotton parcel seed at lamination from experience of life
Reason, this lamination method is conducive to carrying out seed different agent treatments, and for observation, research seed in breaking dormancy mistake
Physiological change in journey is significant.
Claims (4)
1. a kind of method for releasing seed dormancy, it is characterised in that comprise the following steps：
1) seed early stage is handled
Seed is selected with wet cleaning process first, full grains, seed of the same size are selected；Secondly seed is carried out disinfection, prevented and treated
Bacterial invasion, the seed after sterilization is cleaned with water, and seed is finally put into immersion in the beaker for fill water and allows seed fully to absorb water；
2) lamination seed
Seed is wrapped up with absorbent cotton, absorbent cotton is torn into sheet, it is as far as possible thin, it is wrapped in seed coat surface；The seed wrapped is put
Enter and leave certain distance between culture dish, seed, with liquid-transfering gun water suction moistening absorbent cotton；Then culture dish is put into box or use
Masking foil parcel carries out shading treatment；4 DEG C of refrigerator laminations are eventually put into, is showed money or valuables one carries unintentionally with seed and terminates lamination for signal；
3) humidity is controlled
During earthing of seeds, the control of humidity is particularly important, and a water was poured every 3-4 days, only need to be by degreasing when watering
Cotton is soaked, and ponding can not be had by having poured in the water around water liquid-transfering gun absorption seed, culture dish, prevent the mold of seed；
4) rudiment is tested
Bud bed is first carried out during earthing of seeds, two layers of filter paper need to be only padded in culture dish and is moistened, lamination is removed after terminating
The absorbent cotton of seed coat surface, seed is cleaned and is put into ready bud bed, 25 DEG C of thermostatic drying chambers are put into masking foil parcel
It is interior, rudiment test is carried out in dark conditions, daily germination percentage of statistics and moisturizing of watering.
It is specially that hand grabs one that 2. wet cleaning process, which selects seed, according to the method described in claim 1, it is characterised in that step 1)
Seed is put into the beaker for filling water, selects full grains, seed of the same size.
3. with 2%NaClO to seed disinfection according to the method described in claim 1, it is characterised in that step 1), prevent thin
Bacterium is infected, and the seed after sterilization is cleaned 1-3 times with water；Finally seed is put into the beaker for fill water and soaks 24h.
4. change a filter paper within 2-3 days in rudiment test according to the method described in claim 1, it is characterised in that step 4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710224699.3A CN106973581A (en) | 2017-04-07 | 2017-04-07 | A kind of method for releasing pears seed dormancy |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710224699.3A CN106973581A (en) | 2017-04-07 | 2017-04-07 | A kind of method for releasing pears seed dormancy |
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| CN106973581A true CN106973581A (en) | 2017-07-25 |
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| CN201710224699.3A Pending CN106973581A (en) | 2017-04-07 | 2017-04-07 | A kind of method for releasing pears seed dormancy |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646237A (en) * | 2017-10-26 | 2018-02-02 | 铜仁市万山区吊井文祺生态养殖有限公司 | A kind of method for culturing seedlings for breaking pears seed dormancy |
| CN110447338A (en) * | 2019-09-09 | 2019-11-15 | 中国科学院昆明植物研究所 | A method of the Gentianaceae seed after promoting storage is sprouted |
| CN110915512A (en) * | 2019-10-28 | 2020-03-27 | 江西环境工程职业学院 | Seedling raising method for controlling roots of castanea mollissima tannin extracts through efficient bacteriostasis |
| CN111742640A (en) * | 2020-06-08 | 2020-10-09 | 山东省林业科学研究院 | Germination accelerating method for birch pear seeds |
| CN112921069A (en) * | 2021-03-26 | 2021-06-08 | 扬州大学 | Primer group for identifying dormant state of pear seeds, capture probe, identification method and application of primer group |
| CN113748776A (en) * | 2021-08-16 | 2021-12-07 | 河北工程大学 | Method for rapidly inhibiting expression of pear seed NAC transcription factor |
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| CN101563969A (en) * | 2009-06-05 | 2009-10-28 | 南京农业大学 | Method for quickly breaking dormancy of birch-leaf pear seeds with hydroxylamine chloride |
| CN101904236A (en) * | 2010-06-02 | 2010-12-08 | 南京农业大学 | Sprouting method of rapidly removing pyrus betulaefolia seeds from dormancy by using 2-thiopseudourea |
| CN102668769A (en) * | 2012-06-08 | 2012-09-19 | 贵阳中医学院 | Methods for rapidly breaking dormant state of seeds of heterophylly falsestarwort root and performing germination test |
| CN105830583A (en) * | 2015-04-13 | 2016-08-10 | 王小兵 | Method for rapid breaking of Pyrus betulifolia Bunge seed dormancy and promotion of germination |
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| CN101563969A (en) * | 2009-06-05 | 2009-10-28 | 南京农业大学 | Method for quickly breaking dormancy of birch-leaf pear seeds with hydroxylamine chloride |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646237A (en) * | 2017-10-26 | 2018-02-02 | 铜仁市万山区吊井文祺生态养殖有限公司 | A kind of method for culturing seedlings for breaking pears seed dormancy |
| CN110447338A (en) * | 2019-09-09 | 2019-11-15 | 中国科学院昆明植物研究所 | A method of the Gentianaceae seed after promoting storage is sprouted |
| CN110915512A (en) * | 2019-10-28 | 2020-03-27 | 江西环境工程职业学院 | Seedling raising method for controlling roots of castanea mollissima tannin extracts through efficient bacteriostasis |
| CN111742640A (en) * | 2020-06-08 | 2020-10-09 | 山东省林业科学研究院 | Germination accelerating method for birch pear seeds |
| CN112921069A (en) * | 2021-03-26 | 2021-06-08 | 扬州大学 | Primer group for identifying dormant state of pear seeds, capture probe, identification method and application of primer group |
| CN112921069B (en) * | 2021-03-26 | 2023-12-08 | 扬州大学 | Primer group, capture probe and identification method for pear seed dormancy state identification and application of primer group and capture probe |
| CN113748776A (en) * | 2021-08-16 | 2021-12-07 | 河北工程大学 | Method for rapidly inhibiting expression of pear seed NAC transcription factor |
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