CN110447338A - A method of the Gentianaceae seed after promoting storage is sprouted - Google Patents
A method of the Gentianaceae seed after promoting storage is sprouted Download PDFInfo
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- CN110447338A CN110447338A CN201910846281.5A CN201910846281A CN110447338A CN 110447338 A CN110447338 A CN 110447338A CN 201910846281 A CN201910846281 A CN 201910846281A CN 110447338 A CN110447338 A CN 110447338A
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- seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Botany (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention provides a kind of methods for promoting the Gentianaceae seed after storing to sprout, belong to vegetable seeds storage and Germination Technique field, include: by the Gentianaceae seed after storage environment temperature be 1 DEG C of 91~119d of culture, the seed after culture is subjected to sprouting culture.Gentianaceae seed is the germination rate that 1 DEG C of processing 91d~119d improves seed in temperature by the present invention.
Description
Technical field
The invention belongs to the Gentianaceae kinds behind vegetable seeds storage and Germination Technique field more particularly to a kind of promotion storage
The method that son is sprouted.
Background technique
(- 18 DEG C to -20 DEG C) of low temperature storages after orthodox seed drying can greatly be extended into its service life, this is in the world
On built consensus, and multiple crops and Seeds of Wild Plants library are established based on this theory, are stored in low temperature (- 18
DEG C to -20 DEG C) seed in library needs periodically to carry out viability examination, and mainly take seed to sprout the method for experiment；But it is some
Germination characteristics lack research after the especially Seeds of Wild Plants refrigeration of the seed of plant, therefore cannot accurately reflect seed low
Vigour changes situation in warm (- 18 DEG C to -20 DEG C) library.Gentianaceae seed has hibernation feature, without the seed of any processing
Germination rate is very low.
Summary of the invention
In view of this, being adopted the purpose of the present invention is to provide a kind of method for promoting the Gentianaceae seed after storing to sprout
The germination rate of Gentianaceae seed is improved with method provided by the invention.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical schemes:
The present invention provides a kind of methods for promoting the Gentianaceae seed after storing to sprout, comprising: by the rough gentian after storage
Section's seed is 1 DEG C of 90~119d of culture in environment temperature, and the seed after culture is carried out sprouting culture.
Preferably, the Gentianaceae seed includes gentianopsis paludosaMa seed, great bell flower seed, Swertia erythrosticta Maxim seed, the Western Regions
Rough gentian seed, yellowish-white Gentianopsis barbata seed, convolution one or more of Gentianopsis barbata seed and ovum leaf Gentianopsis barbata seed.
Preferably, the temperature of the storage is -18~-20 DEG C.
Preferably, the Gentianaceae seed includes: by the Gentianaceae seed before culture from -18~-20 DEG C of storage items
Under part take out after environment temperature be 15 DEG C, relative humidity be 15% processing for 24 hours.
Preferably, the culture further include: the intensity of illumination of the culture is 1000~5000 luxs, the culture
Photoperiod is 12h illumination 12h dark.
Preferably, the cultivation temperature of the illumination is 1 DEG C, and the cultivation temperature of the dark is 1 DEG C.
Preferably, it is 1000~5000 Le gram that the condition for sprouting culture, which includes: the intensity of illumination for sprouting culture,
This, the photoperiod for sprouting culture is 12h illumination 12h dark.
Preferably, the cultivation temperature of the illumination is 25 ± 2 DEG C, and the cultivation temperature of the dark is 15 ± 2 DEG C.
Preferably, the water content of the Gentianaceae seed is lower than 10%.
The present invention provides a kind of methods for promoting the Gentianaceae seed after storing to sprout, comprising: by the rough gentian after storage
Section's seed is 1 DEG C of 91~119d of culture in environment temperature, and the seed after culture is carried out sprouting culture.The present invention is by Gentianaceae kind
Son is in the germination rate that temperature is that 1 DEG C of processing 91d~119d improves seed.
Specific embodiment
The present invention provides a kind of methods for promoting the Gentianaceae seed after storing to sprout, comprising: by the rough gentian after storage
Section's seed is 1 DEG C of 91~119d of culture in environment temperature, and the seed after culture is carried out sprouting culture.
In the present invention, the Gentianaceae seed preferably includes gentianopsis paludosaMa seed, great bell flower seed, Swertia erythrosticta Maxim kind
Son, Western Regions rough gentian seed, yellowish-white Gentianopsis barbata seed, convolution one or more of Gentianopsis barbata seed and ovum leaf Gentianopsis barbata seed.In this hair
In bright, the temperature of the storage is preferably -18 DEG C~-20 DEG C.It is 15 that the Gentianaceae seed is preferably placed in temperature by the present invention
DEG C, in the environment of relative humidity 15% until the equilibrium relative humidity (ERH) of seed reaches 15%, seed moisture content is lower than 10% at this time,
Then seed is put into closed container again, is stored in -18 DEG C to -20 DEG C.The present invention does not have the source of the Gentianaceae seed
There is particular determination.
In the present invention, the Gentianaceae seed includes: to store up the Gentianaceae seed from -18~-20 DEG C before culture
Under the conditions of hiding take out after environment temperature be 15 DEG C, relative humidity be 15% processing for 24 hours.The culture further preferably includes: described
The intensity of illumination of culture is preferably 1000~5000 luxs, and the photoperiod of the culture is preferably 12h illumination 12h dark；Institute
The cultivation temperature for stating illumination is preferably 1 DEG C, and the cultivation temperature of the dark is preferably 1 DEG C.
In the present invention, the condition for sprouting culture preferably includes: the intensity of illumination for sprouting culture is preferably
1000~5000 luxs, the photoperiod for sprouting culture are preferably 12h illumination 12h dark；The cultivation temperature of the illumination
Preferably 25 ± 2 DEG C, the cultivation temperature of the dark is preferably 15 ± 2 DEG C.The present invention preferably carries out sprouting training in the incubator
It supports, the present invention is not particularly limited the specification etc. of the incubator, using the specification of routine culture case.
Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.
Embodiment 1
Acquire mature fresh gentianopsis paludosaMa (Gentianopsis paludosa (Munro ex J.D.Hooker) Ma)
Seed transports Kunming Institute of Zoology Southwest China wildlife germplasm resource bank germplasm collection back after acquisition, amount to 18 parts.
Table 1 acquires information table
Rising again after dry refrigeration and refrigeration: collected seed is placed in 15 DEG C, in the drying room of 15% relative humidity
It is dry.Same company is periodically combined with the Hygrolab C1 unit relative humidity analyzer of Rotronic Ltd. company production
The Rotronic HC 2-AW probe of production is measured seed relative humidity, and ready to balance relative humidity is packed into after reaching 15%
In closed container.Seed-bearing closed container will be filled and be put into -18 DEG C to -20 DEG C refrigerations.It will be equipped with kind after stored for some time
The closed container of son takes out.15 DEG C are put into, is opened after rising again within 24 hours in the drying room of 15% relative humidity and fills seed-bearing appearance
Device, random take out test required seed.
By seed sowing on 1% agar medium, being placed on temperature is 1 DEG C, and intensity of illumination is 1000 luxs, light week
Phase is that seed is moved to 25/15 DEG C together with 1% agar medium in the dark incubator in 12 hours illumination/12 hour after 91 days,
1000 luxs, photoperiod are to sprout in the dark incubator in 12 hours illumination/12 hour.
Each processing method sows 20 seeds, checks primary sprouting at interval of 7 days, radicle extends over 2mm and thinks
Seed is sprouted, and germination seed is taken out, and record sprouting quantity.
Germination rate %=germination seed number/seed sum * 100
It is analyzed using SPSS16.0 software data are sprouted, data variance is analyzed using Chi-square.As a result
It is shown in Table 2.
Embodiment 2
By seed sowing on 1% agar medium, being placed on temperature is 1 DEG C, and intensity of illumination is 1000 luxs, light week
Phase is that seed is moved to 25/15 together with 1% agar medium in the dark incubator in 12 hours illumination/12 hour after 119 days
DEG C, intensity of illumination is 1000 luxs, and the photoperiod is to sprout in the dark incubator in 12 hours illumination/12 hour, be the results are shown in Table
2.Other conditions and seed source are the same as embodiment 1.
Comparative example 1
Seed is seeded on 1% agar medium containing 200 milligrams per liter of gibberellin, is placed in 15 DEG C of temperature, light
It is 1000 luxs according to intensity, the photoperiod is to sprout in the dark incubator in 12 hours illumination/12 hour, the results are shown in Table 2.It is other
Condition and seed source are the same as embodiment 1.
Comparative example 2
By seed sowing on 1% agar medium, being placed on temperature is 5 DEG C, and intensity of illumination is 1000 luxs, light week
Phase is that seed is moved to 25/15 DEG C together with 1% agar medium in the dark incubator in 12 hours illumination/12 hour after 91 days,
Intensity of illumination is 1000 luxs, and the photoperiod is to sprout in the dark incubator in 12 hours illumination/12 hour, the results are shown in Table 2.Its
Its condition and seed source are the same as embodiment 1.
Comparative example 3
By seed sowing on 1% agar medium, being placed on temperature is 5 DEG C, and intensity of illumination is 1000 luxs, light week
Phase is that seed is moved to 25/15 together with 1% agar medium in the dark incubator in 12 hours illumination/12 hour after 119 days
DEG C, intensity of illumination is 1000 luxs, and the photoperiod is to sprout in the dark incubator in 12 hours illumination/12 hour, be the results are shown in Table
2.Other conditions and seed source are the same as embodiment 1.
Gentianopsis paludosaMa seed germination rate (%) after table 2 refrigerates
Different letters indicate that the germination rate between different disposal has significant difference
Show that the seed after refrigerating at -20 DEG C takes comparative example 1, comparative example 2 and comparative example by Chi-Square inspection
3 germination rate is lower, and the germination rate of embodiment 1 and embodiment 2 is significantly increased.Illustrate 200 milligrams per liter of gibberellin and 5
Seed dormancy cannot be broken for 91 days and 119 days by DEG C placing, and seed is promoted to sprout.The germination rate of embodiment 1 and embodiment 2 and its
Its method has extremely significant difference, but therebetween without significant difference, illustrating to place 91 days and 119 days for 1 DEG C can be significantly improved
Seed germination rate, operation in 91 days are more simple and easy to do.Embodiment 1 and embodiment 2 are for picking up from different regions and height above sea level (2323
- 4525 meters of rice), the gentianopsis paludosaMa seed breaking dormancy of Different hypothermia Storage Period of Time (- 7.29 years 3.33) promotes sprouting to have
There is remarkable result, illustrates that this method use scope is wider.
Embodiment 3
Mature Gentianaceae fresh seeds are acquired, transport Kunming Institute of Zoology Southwest China wildlife germplasm after acquisition back
Resources bank germplasm collection amounts to 7 parts of 6 species, is shown in Table 3.
Table 3 acquires information table
Rising again after dry refrigeration and refrigeration: collected seed is placed in 15 DEG C, in the drying room of 15% relative humidity
It is dry.Same company is periodically combined with the Hygrolab C1 unit relative humidity analyzer of Rotronic Ltd. company production
The Rotronic HC 2-AW probe of production is measured seed relative humidity, and ready to balance relative humidity is packed into after reaching 15%
In closed container.Seed-bearing closed container will be filled and be put into -18 DEG C to -20 DEG C refrigerations.It will be equipped with kind after stored for some time
The closed container of son takes out.15 DEG C are put into, is opened after rising again within 24 hours in the drying room of 15% relative humidity and fills seed-bearing appearance
Device, random take out test required seed.
By seed sowing on 1% agar medium, being placed on temperature is 1 DEG C, and intensity of illumination is 1000 luxs, light week
Phase is that seed is moved to 25/15 DEG C together with 1% agar medium in the dark incubator in 12 hours illumination/12 hour after 91 days,
Intensity of illumination is 1000 luxs, and the photoperiod is to sprout in the dark incubator in 12 hours illumination/12 hour.
Each processing method sows 20 seeds, checks primary sprouting at interval of 7 days, radicle extends over 2mm and thinks
Seed is sprouted, and germination seed is taken out, and record sprouting quantity.
Germination rate %=germination seed number/seed sum * 100
It is analyzed using SPSS16.0 software data are sprouted, data variance is analyzed using Chi-square.As a result
It is shown in Table 4.
Embodiment 4
By seed sowing on 1% agar medium, be placed on temperature be 1 DEG C, intensity of illumination be 1000 grams this, the photoperiod
For after 119 days, seed is moved to 25/15 DEG C together with 1% agar medium in the dark incubator in 12 hours illumination/12 hour,
Intensity of illumination is 1000 luxs, and the photoperiod is to sprout in the dark incubator in 12 hours illumination/12 hour, the results are shown in Table 4.Its
Its condition and seed source are the same as embodiment 3.
Comparative example 4
Seed is seeded on 1% agar medium containing 200 milligrams per liter of gibberellin, is placed in 15 DEG C of temperature, light
It is 1000 luxs according to intensity, the photoperiod is to sprout in the dark incubator in 12 hours illumination/12 hour, the results are shown in Table 4.It is other
Condition and seed source are the same as embodiment 3.
Comparative example 5
By seed sowing on 1% agar medium, being placed on temperature is 5 DEG C, and intensity of illumination is 1000 luxs, light week
Phase is that seed is moved to 25/15 DEG C together with 1% agar medium in the dark incubator in 12 hours illumination/12 hour after 91 days,
Intensity of illumination is 1000 luxs, and the photoperiod is to sprout in the dark incubator in 12 hours illumination/12 hour, the results are shown in Table 4.Its
Its condition and seed source are the same as embodiment 3.
Comparative example 6
By seed sowing on 1% agar medium, being placed on temperature is 5 DEG C, and intensity of illumination is 1000 luxs, light week
Phase is that seed is moved to 25/15 together with 1% agar medium in the dark incubator in 12 hours illumination/12 hour after 119 days
DEG C, intensity of illumination is 1000 luxs, and the photoperiod is to sprout in the dark incubator in 12 hours illumination/12 hour, be the results are shown in Table
4.Other conditions and seed source are the same as embodiment 3.
Seed germination rate (%) after table 4 refrigerates
Different letters indicate that the germination rate between different disposal has significant difference
Show that the seed after refrigerating at -20 DEG C takes comparative example 4, comparative example 5 and comparative example by Chi-Square inspection
6 germination rate is lower, and the germination rate of embodiment 3 and embodiment 4 is significantly increased.Illustrate 200 milligrams per liter of gibberellin and 5
Seed dormancy cannot be broken for 91 days and 119 days by DEG C placing, and seed is promoted to sprout.The germination rate of embodiment 3 and embodiment 4 and its
His method has extremely significant difference, but therebetween without significant difference, illustrating to place 91 days and 119 days for 1 DEG C can be significantly improved
Seed germination rate, operation in 91 days are more simple and easy to do.Embodiment 3 and embodiment 4 all have remarkable result for 6 species, say
Bright this method use scope is wider.
By above embodiments it can be concluded that, the Gentianaceae seed after cryopreservation is improved using method provided by the invention
Germination rate.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of method for promoting the Gentianaceae seed sprouting after storage characterized by comprising by the Gentianaceae kind after storage
Son is 1 DEG C of 91~119d of culture in environment temperature, and the seed after culture is carried out sprouting culture.
2. the method according to claim 1, wherein the Gentianaceae seed includes gentianopsis paludosaMa seed, great bell
In flower seed, Swertia erythrosticta Maxim seed, Western Regions rough gentian seed, yellowish-white Gentianopsis barbata seed, convolution Gentianopsis barbata seed and ovum leaf Gentianopsis barbata seed
One or more.
3. the method according to claim 1, wherein the temperature of the storage is -18~-20 DEG C.
4. the method according to claim 1, wherein the Gentianaceae seed includes: by the dragon before culture
Gallbladder section seed taken out under -18~-20 DEG C of storage conditions after environment temperature be 15 DEG C, relative humidity be 15% processing for 24 hours.
5. the method according to claim 1, wherein the culture further include: the intensity of illumination of the culture is
1000~5000 luxs, the photoperiod of the culture are 12h illumination 12h dark.
6. according to the method described in claim 5, it is characterized in that, the cultivation temperature of the illumination be 1 DEG C, the training of the dark
Supporting temperature is 1 DEG C.
7. the method according to claim 1, wherein the condition for sprouting culture includes: the sprouting culture
Intensity of illumination be 1000~5000 luxs, the photoperiod for sprouting culture is that 12h illumination 12h is dark.
8. the method according to the description of claim 7 is characterized in that the cultivation temperature of the illumination be 25 ± 2 DEG C, the dark
Cultivation temperature be 15 ± 2 DEG C.
9. the method according to claim 1, wherein the water content of the Gentianaceae seed is lower than 10%.
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