CN107027372A - A kind of method for promoting red gentian seed to sprout and its seedling - Google Patents
A kind of method for promoting red gentian seed to sprout and its seedling Download PDFInfo
- Publication number
- CN107027372A CN107027372A CN201611009376.4A CN201611009376A CN107027372A CN 107027372 A CN107027372 A CN 107027372A CN 201611009376 A CN201611009376 A CN 201611009376A CN 107027372 A CN107027372 A CN 107027372A
- Authority
- CN
- China
- Prior art keywords
- seed
- red gentian
- sprout
- gentian
- red
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C5/00—Fertilisers containing other nitrates
- C05C5/02—Fertilisers containing other nitrates containing sodium or potassium nitrate
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a kind of method for promoting red gentian seed to sprout and its seedling, it is related to vegetable seeds processing technology field.What the present invention was provided promotes the method that red gentian seed is sprouted, including preparing the step such as seed soaking and sprouting budding before seed treatment liquid, seed are sprouted, rarely have people to be related to for the research in terms of current red gentian seed sprouting, situation more without large area artificial growth red gentian, there is provided the sprouting germination method of a set of original creation, provided the foundation for the artificial growth of red gentian, and then promote the sustainable use of red gentian resource.
Description
Technical field
The present invention relates to vegetable seeds process field, in particular to a kind of side for promoting red gentian seed to sprout
Method and its seedling.
Background technology
Red gentian Gentiana rhodantha, also known as felwort, Tu Bailian, flower in September, star show flower, cold wind are blown or avenged
In plum, be Gentianaceae agueweed, be grown on alpine scrub, meadow and the sylvan life of 570~1750 meters of height above sea level, be《Middle Chinese
People republic pharmacopeia》Version newly records kind within 2015.Red gentian is with root and all herbal medicine, and bitter is cold in nature, Return liver, gallbladder channel, tool
There is the effect of clearing heat and promoting diuresis, inflammation-diminishing and cough-controlling, removing toxic substances.Tcm clinical practice is usually used in acute icteric hepatitis, dysentery, infantile pneumonia, branch
Carbuncle furuncle sore is controlled in tracheitis, bronchial astehma, pulmonary tuberculosis, scrofula, difficult urination, eye conjunctivitis and external application, is burnt and is scalded
The illnesss such as wound.
Red gentian is typical acrophyta, likes Cold and cool climate, there is stronger cold resistance；Will to temperature in growth course
Ask not strict, but during seed sprouting, it is necessary to there are suitable temperature and certain illumination condition, otherwise seed germination rate is very low,
Through seriously limiting research and utilization of the people to this good resource.
Rarely have people to be related to the research in terms of red gentian seed sprouting at present, more without large area artificial growth safflower dragon
The precedent of courage.Therefore, pushing away for the sustainable use of promotion red gentian resource, improving quality and red gentian improved seeds
Extensively, the research in terms of progress red gentian sprouting seems very necessary.
In view of this, it is special to propose the present invention.
The content of the invention
The first object of the present invention is to provide a kind of method for promoting red gentian seed to sprout, the red gentian kind
Sub- germination method by using seed treatment liquid before sprouting to seed carry out seed soaking, can be rapidly and efficiently abolish red gentian
Seed dormancy simultaneously makes its germination rate in 10~20 days be higher by 10~30%, and germination than distilled water seed soaking sprouting processing
Neatly, the time of red gentian seed breeding is saved.
The second object of the present invention is to provide a kind of red gentian seedling, and the red gentian seedling is red by above-mentioned promotion
The method that flower rough gentian seed is sprouted is made, and its emergence rate is high, and Phase ofsprouting is uniform.
The method that a kind of promotion red gentian seed that the present invention is provided is sprouted, methods described comprises the following steps：
(1) seed treatment liquid is prepared：Prepare potassium nitrate, calcium chloride or Gibberellins solution and be used as seed treatment liquid；
(2) seed soaking before seed is sprouted：By red gentian seed gauze wrapped, step (1) seed treatment liquid is placed in
In, soak seed 20~30 hours, soaked seed 10~15 hours in the environment of being subsequently placed into 3~5 DEG C, then in the environment of 18~22 DEG C
Seed-soaking liquid is abandoned, wash seeds are clean, complete seed soaking；
(3) budding is sprouted：Seed after step (2) seed soaking is put into and is covered with the culture dish of moistening filter paper, then
It is put into incubator and cultivates 10~20 days, and keeps filter paper to moisten, the red gentian seed after being germinateed.
Further, step (4) sprouting is optionally carried out to emerge：By the red gentian seed after step (2) seed soaking
Mixed thoroughly with plant ash, be uniformly seeded in progress sprouting on seedbed and emerge 90~120 days, obtain red gentian seedling.
Further, potassium nitrate solution mass percentage concentration is 1%~5% in above-mentioned steps (1)；In above-mentioned steps (1)
Calcium chloride solution mass percentage concentration is 0.25%~2%；Gibberellins solution mass-volume concentration is in above-mentioned steps (1)
50mg/L~800mg/L.
Further, the method for above-mentioned steps (2) wash seeds is to use distilled water flushing 4~5 times, every time 3~8 minutes.
Further, incubator is illumination box in above-mentioned steps (3), and the cultivation temperature of above-mentioned illumination box is 23
~28 DEG C, intensity of illumination is 2~4 grades, 12 hours dark alternatings of every illumination in 12 hours.
Further, after above-mentioned steps (2) seed soaking red gentian seed and the mass ratio of plant ash is 1:500~
1000。
Further, red gentian seed after above-mentioned steps (2) seed soaking is sowed first 2~4 days, with 0.05~
0.1% liquor potassic permanganate is uniformly splashed in seedbed, and soil is carried out disinfection, and is irrigated prior to seeding with clear water within 2~3 hours
Seedbed.
Further, the environmental requirement that above-mentioned sprouting is emerged be soil moisture content 45~65%, earth's surface air humidity 60~
70%, 20~25 DEG C of temperature.
Further, the method that above-mentioned promotion red gentian seed is sprouted, methods described specifically includes following steps：
(1) seed treatment liquid is prepared：Prepare potassium nitrate, calcium chloride or Gibberellins solution and be used as seed treatment liquid；
The potassium nitrate solution mass percentage concentration is 1%, 2%, 3%, 4% and 5%；
The calcium chloride solution mass percentage concentration is 0.25%, 0.5%, 1%, 1.5% and 2%；
The Gibberellins solution mass-volume concentration is 50mg/L, 100mg/L, 200mg/L, 400mg/L and 800mg/L；
(2) seed soaking before seed is sprouted：By red gentian seed with 2~3 layers of gauze wrapped, step (1) seed is placed in
In treatment fluid, soaked seed 20~30 hours in the environment of 18~22 DEG C, seed soaking 10~15 is small in the environment of being subsequently placed into 3~5 DEG C
When, seed-soaking liquid is then abandoned, by seed distilled water flushing 4~5 times, 3~8 minutes every time, seed soaking is completed；
(3) budding is sprouted：Seed after step (2) seed soaking is put into the culture dish for being covered with 2~3 layers of moistening filter paper
On, it is subsequently placed into illumination box, is 23~28 DEG C in temperature, intensity of illumination is 2~4 grades, every illumination in 12 hours 12 hours
Dark alternate culture 10~20 days, and keep filter paper to moisten, the red gentian seed after being germinateed；
Optionally carry out,
(4) sprout and emerge：By the red gentian seed after step (2) seed soaking and plant ash with 1:500~1000
Mass ratio is mixed thoroughly, is uniformly seeded in progress sprouting on seedbed and is emerged 90~120 days, it is soil water-containing to sprout the environmental requirement emerged
Amount 45~65%, earth's surface air humidity 60~70%, 20~25 DEG C of temperature obtains red gentian seedling.
A kind of red gentian seedling that the present invention is provided, the red gentian seedling is by above-mentioned promotion red gentian seed
The method of sprouting is made.
Compared with prior art, beneficial effects of the present invention are：
(1) method that red gentian seed is sprouted that what the present invention was provided promote, above-mentioned seed germination method is by by nitric acid
Potassium, calcium chloride or Gibberellins solution carry out seed soaking, first at 18~22 DEG C as seed treatment liquid before seed is sprouted
Soak seed 20~30 hours, soaked seed 10~15 hours in the environment of being subsequently placed into 3~5 DEG C under environment, then carried out in incubator
12 hours dark of every illumination in 12 hours are alternate incubated, and the germination percentage of obtained red gentian seed is very good, seed
Germination percentage be higher by 20~30% than the soak seed method sprouted of application distilled water, solve red gentian and planted as high mountain
Thing, when its seed is sprouted, it is impossible to rapidly and efficiently abolish red gentian seed dormancy, and the environment in incubation is sprouted
Temperature, the condition such as illumination is difficult to control, and causes the germination percentage of seed very low, the problems such as breeding time is long, and finally red to promote
The sustainable use of flower rough gentian resource provides the foundation.
(2) the red gentian seedling that the present invention is provided, the red gentian seedling is sprouted by above-mentioned promotion red gentian seed
The method of hair is made, the emergence rate for the seed that its emergence rate is emerged apparently higher than after being sprouted with distilled water, and seed sprouting
Neatly.
Embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the present invention.It is unreceipted specific in embodiment
Condition person, the condition advised according to normal condition or manufacturer is carried out.Agents useful for same or the unreceipted production firm person of instrument, be
The conventional products that can be obtained by commercially available purchase.
The method for promoting red gentian seed to sprout a kind of according to an aspect of the present invention, methods described includes following step
Suddenly：
(1) seed treatment liquid is prepared：Prepare potassium nitrate, calcium chloride or Gibberellins solution and be used as seed treatment liquid；
(2) seed soaking before seed is sprouted：By red gentian seed with 2~3 layers of gauze wrapped, step (1) seed is placed in
In treatment fluid, soaked seed 20~30 hours in the environment of 18~22 DEG C, seed soaking 10~15 is small in the environment of being subsequently placed into 3~5 DEG C
When, seed-soaking liquid is then abandoned, wash seeds are clean, complete seed soaking；
(3) culture is sprouted：Seed after step (2) seed soaking is put into and is covered with the culture dish of moistening filter paper, then
It is put into incubator and cultivates 10~20 days, and keeps filter paper to moisten, the red gentian seed after being germinateed.
In the present invention, above-mentioned potassium nitrate belongs to binary compound fertilizer.Its nutrient for plants potassium, the total content of nitrogen are up to 50% left side
The right side, with good physicochemical properties.The potassium nitrate of certain concentration is applied in red gentian Seed Germination of the present invention
Solution carries out processing to seed has breaking dormancy, improves seed vitality, improves the effect of emergence rate.K in potassium nitrate+As
The activator of a variety of enzymes (such as NAD kinases, ATP enzyme), can improve or promote the activity of a variety of enzymes to a certain extent, also make
Cell membrane obtains part reparation, may also participate in the biosynthesis for promoting sex hormone in induction plant and its vigor regulation and control, so that
Red gentian seed vigor is improved, stratification.
In the present invention, above-mentioned calcium chloride participates in and maintained the metabolic activity of organism as the constituent of cell, maintains
Acid-base balance in organism, maintains the osmotic pressure of cell, is played an important role particularly with plant absorption nutrient.In calcium chloride
Calcium ion is both a kind of nutrient, but also is to be coupled extracellular signal and the second messenger of intracellular biochemical reactions, to planting
Growing for thing has important regulating and controlling effect.
In the present invention, above-mentioned gibberellin is applied to the sprouting of red gentian seed, gibberellin as a Plant Hormone
The content of auxin in plant can be improved, and then accelerates the elongation of cell, also has facilitation to the division of cell, it can
To promote the expansion of cell, in addition, gibberellin also has the dormancy effect of suppression lateral bud.
In the present invention, above-mentioned red gentian seed is complete, the undamaged filbert mature seed by screening, above-mentioned
Red gentian seed is to choose long 2.275~1.550mm, wide 1.900~1.125mm, seed length-width ratio 1.444~1.000,
The seed that every thousand seed weights are 0.1296~0.1248g.
In the preferred embodiment of the present invention, step (4) sprouting is optionally carried out to emerge：By step (2) seed soaking
Red gentian seed after processing is mixed thoroughly with plant ash, is uniformly seeded in progress sprouting on seedbed and is emerged 90~120 days, obtains red
Flower rough gentian seedling.
In the preferred embodiment of the present invention, potassium nitrate solution mass percentage concentration is 1% in above-mentioned steps (1)
~5%；Calcium chloride solution mass percentage concentration is 0.25%~2% in above-mentioned steps (1)；Gibberellin is molten in above-mentioned steps (1)
Liquid mass-volume concentration is 50mg/L~800mg/L.
In the present invention, by the further adjustment of the concentration ratio to above-mentioned potassium nitrate, calcium chloride and Gibberellins solution and
Optimization, so as to further optimize the effect that red gentian seed of the present invention is sprouted.
In the preferred embodiment of the present invention, the method for above-mentioned steps (2) wash seeds is to use distilled water flushing 4
~5 times, every time 3~8 minutes.
In the preferred embodiment of the present invention, incubator is illumination box, illumination cultivation in above-mentioned steps (3)
The cultivation temperature of case is 23~28 DEG C, and intensity of illumination is 2~4 grades, 12 hours dark alternatings of every illumination in 12 hours.
In the preferred embodiment of the present invention, the red gentian seed after above-mentioned steps (2) seed soaking and grass
The mass ratio of wood ash is 1:500~1000.
In the preferred embodiment of the present invention, the red gentian seed sowing after above-mentioned steps (2) seed soaking
First 2~4 days, uniformly splashed in seedbed with 0.05~0.1% liquor potassic permanganate, soil is carried out disinfection, and prior to seeding 2
Seedbed is irrigated with clear water within~3 hours.
It is above-mentioned that seedbed is carried out disinfection with liquor potassic permanganate before sprouting is emerged, it can effectively kill the disease in soil
Bacterium, makes red gentian preferably emerge growth, it is to avoid the generation of seedling stage diseases and pests.
In the preferred embodiment of the present invention, the environmental requirement that above-mentioned sprouting is emerged be soil moisture content 45~
65%, earth's surface air humidity 60~70%, 20~25 DEG C of temperature.
The present invention is sprouted in the incubation emerged, and seedbed is watered in good time according to soil moisture, temperature, weather condition,
Ground moistening is kept, general fine day once a day, 2~3 days cloudy days, once do not watered by the rainy day, periodically removes weeds, insect prevention, fixed
When observation, record.
In above-mentioned preferred embodiment, build a sunshade net on seedbed, the degree of shading of sunshade net for 60%~
70%.
In the preferred embodiment of the present invention, methods described specifically includes following steps：
(1) seed treatment liquid is prepared：Prepare potassium nitrate, calcium chloride or Gibberellins solution and be used as seed treatment liquid；
The potassium nitrate solution mass percentage concentration is 1%, 2%, 3%, 4% and 5%；
The calcium chloride solution mass percentage concentration is 0.25%, 0.5%, 1%, 1.5% and 2%；
The Gibberellins solution mass-volume concentration is 50mg/L, 100mg/L, 200mg/L, 400mg/L and 800mg/L；
(2) seed soaking before seed is sprouted：By red gentian seed with 2~3 layers of gauze wrapped, step (1) seed is placed in
In treatment fluid, soaked seed 20~30 hours in the environment of 18~22 DEG C, seed soaking 10~15 is small in the environment of being subsequently placed into 3~5 DEG C
When, seed-soaking liquid is then abandoned, by seed distilled water flushing 4~5 times, 3~8 minutes every time, seed soaking is completed；
(3) culture is sprouted：Seed after step (2) seed soaking is put into the culture dish for being covered with 2~3 layers of moistening filter paper
On, it is subsequently placed into illumination box, is 23~28 DEG C in temperature, intensity of illumination is 2~4 grades, every illumination in 12 hours 12 hours
Dark alternate culture 10~20 days, and keep filter paper to moisten, the red gentian seed after being germinateed；
Optionally carry out,
(4) sprout and emerge：By the red gentian seed after step (2) seed soaking and plant ash with 1:500~1000
Mass ratio is mixed thoroughly, is uniformly seeded in progress sprouting on seedbed and is emerged 90~120 days, it is soil water-containing to sprout the environmental requirement emerged
Amount 45~65%, earth's surface air humidity 60~70%, 20~25 DEG C of temperature obtains red gentian seedling.
In above-mentioned preferred embodiment, it is preferable that methods described specifically includes following steps：
(1) seed treatment liquid is prepared：Prepare potassium nitrate, calcium chloride or Gibberellins solution and be used as seed treatment liquid；
The potassium nitrate solution mass percentage concentration is 1%, 2%, 3%, 4%, 5%；
The calcium chloride solution mass percentage concentration is 0.25%, 0.5%, 1%, 1.5% and 2%；
The Gibberellins solution mass-volume concentration is 50mg/L, 100mg/L, 200mg/L, 400mg/L and 800mg/L；
(2) seed soaking before seed is sprouted：By red gentian seed with 2 layers of gauze wrapped, step (1) seed treatment is placed in
In liquid, soaked seed 24 hours in the environment of 20 DEG C, soaked seed 12 hours in the environment of being subsequently placed into 4 DEG C, then abandon seed-soaking liquid, will plant
Son uses distilled water flushing 4 times, 5 minutes every time, completes seed soaking；
(3) culture is sprouted：Seed after step (2) seed soaking is put into and is covered with the culture dish of 2 layers of moistening filter paper, with
After be put into illumination box, be 25 DEG C in temperature, intensity of illumination is 3 grades, the dark alternate culture of every illumination in 12 hours 12 hours
18 days, and keep filter paper to moisten, the red gentian seed after being germinateed；
Optionally carry out,
(4) sprout and emerge：By the red gentian seed after step (2) seed soaking and plant ash with 1:500~1000
Mass ratio is mixed thoroughly, is uniformly seeded in progress sprouting on seedbed and is emerged 90~120 days, it is soil water-containing to sprout the environmental requirement emerged
Amount 45~65%, earth's surface air humidity 60~70%, 20~25 DEG C of temperature obtains red gentian seedling.
According to an aspect of the present invention, a kind of red gentian seedling, the red gentian seedling is red by above-mentioned promotion
The method that flower rough gentian seed is sprouted is made.
Embodiment 1
A kind of method for promoting red gentian seed to sprout, methods described comprises the following steps：
(1) 450 red gentian seeds are chosen, are divided into three groups, every group of 150 red gentian seeds are then each with 2 layers of yarn
Cloth is wrapped up, and is respectively placed in the potassium nitrate seed treatment liquid that concentration is 1%, 2% and 3%, wherein,
First group is placed in 1% potassium nitrate seed treatment liquid；
Second group is placed in 2% potassium nitrate seed treatment liquid；
3rd group is placed in 3% potassium nitrate seed treatment liquid；
Then three groups of seeds are soaked seed 24 hours in the environment of 20 DEG C, soaked seed 12 hours in the environment of being subsequently placed into 4 DEG C,
Abandon seed-soaking liquid；
(2) three groups of seed distilled water flushings 4 times after seed-soaking liquid will be abandoned, 5 minutes every time, seed soaking are completed；
(3) seed after above-mentioned three groups of steps (2) seed soaking is respectively put into the culture dish for being covered with 2 layers of moistening filter paper
On, it is subsequently placed into incubator and cultivates 20 days, and keep filter paper to moisten, the red gentian seed after being germinateed；
(4) in above-mentioned steps (3) incubator incubation, to Continuous Observation in the seed germination phase, and respectively to three groups
The germinative number of seed is counted, and calculates red gentian percentage of seedgermination, germinating energy and germination index.
Embodiment 2
A kind of method for promoting red gentian seed to sprout, methods described comprises the following steps：
(1) 750 red gentian seeds are chosen, are divided into five groups, every group of 150 red gentian seeds are then each with 2 layers of yarn
Cloth is wrapped up, and is respectively placed in the calcium chloride seed treatment liquid that concentration is 0.25%, 0.5%, 1%, 1.5% and 2%, wherein,
First group is placed in 0.25% calcium chloride seed treatment liquid；
Second group is placed in 0.5% calcium chloride seed treatment liquid；
3rd group is placed in 1% calcium chloride seed treatment liquid；
4th group is placed in 1.5% calcium chloride seed treatment liquid；
5th group is placed in 2% calcium chloride seed treatment liquid,
Then five groups of seeds are soaked seed 24 hours under 20 DEG C of environment, soaked seed 12 hours in the environment of being subsequently placed into 4 DEG C, so
After abandon seed-soaking liquid；
(2) five groups of seed distilled water flushings 4 times after seed-soaking liquid will be abandoned, 5 minutes every time, seed soaking are completed；
(3) seed after above-mentioned five groups of steps (2) seed soaking is respectively put into the culture dish for being covered with 2 layers of moistening filter paper
On, it is subsequently placed into incubator and cultivates 20 days, and keep filter paper to moisten, the red gentian seed after being germinateed；
(4) in above-mentioned steps (3) incubator incubation, to Continuous Observation in the seed germination phase, and respectively to five groups
The germinative number of seed is counted, and calculates red gentian percentage of seedgermination, germinating energy and germination index.
Embodiment 3
A kind of method for promoting red gentian seed to sprout, methods described comprises the following steps：
(1) 750 red gentian seeds are chosen, are divided into five groups, every group of 150 red gentian seeds are then each with 2 layers of yarn
Cloth is wrapped up, and is respectively placed in the gibberellin seed treatment that concentration is 50mg/L, 100mg/L, 200mg/L, 400mg/L and 800mg/L
In liquid, wherein,
First group be placed in 50mg/L gibberellin seed treatment liquid；
Second group is placed in 100mg/L gibberellin seed treatment liquid；
3rd group is placed in 200mg/L gibberellin seed treatment liquid；
4th group is placed in 400mg/L gibberellin seed treatment liquid；
5th group is placed in 800mg/L gibberellin seed treatment liquid,
Then five groups of seeds are soaked seed 24 hours under 20 DEG C of environment, soaked seed 12 hours in the environment of being subsequently placed into 4 DEG C, so
After abandon seed-soaking liquid；
(2) five groups of seed distilled water flushings 4 times after seed-soaking liquid will be abandoned, 5 minutes every time, seed soaking are completed；
(3) seed after above-mentioned five groups of steps (2) seed soaking is respectively put into the culture dish for being covered with 2 layers of moistening filter paper
On, it is subsequently placed into incubator and cultivates 20 days, and keep filter paper to moisten, the red gentian seed after being germinateed；
(4) in above-mentioned steps (3) incubator incubation, to Continuous Observation in the seed germination phase, and respectively to five groups
The germinative number of seed is counted, and calculates red gentian percentage of seedgermination, germinating energy and germination index.
Comparative example 1
A kind of method for promoting red gentian seed to sprout, methods described comprises the following steps：
(1) 150 red gentian seeds are taken, then with 2 layers of gauze wrapped, are placed in distilled water, are soaked under 20 DEG C of environment
Plant 24 hours, soaked seed 12 hours in the environment of being subsequently placed into 4 DEG C, then abandon seed-soaking liquid；
(2) distilled water flushing 4 times of the seed after seed-soaking liquid will be abandoned, 5 minutes every time, seed soaking is completed；
(3) seed after above-mentioned steps (2) seed soaking is put into and be covered with the culture dish of 2 layers of moistening filter paper, then put
Enter in incubator and cultivate 20 days, and keep filter paper to moisten, the red gentian seed after being germinateed；
(4) in above-mentioned steps (3) incubator incubation, to Continuous Observation in the seed germination phase, and germinative number is entered
Row statistics, calculates red gentian percentage of seedgermination, germinating energy and germination index.
Effect example 1
For show method that the present invention promotes red gentian seed to sprout have rapidly and efficiently abolish red gentian seed
Dormancy, improves percentage of seedgermination, shortens the technique effect of seed breeding time.
Special selection example 1~3 and comparative example 1, germination percentage, germinating energy, germination index, hair after being sprouted for its seed
Bud time lag and germination last carries out sprouting comparative evaluation to the embodiment soaked seed using different reagent concentrations, and result of the test is as follows
Shown in table 1：
Table 1：The influence that different reagent concentration seed soaking are sprouted to red gentian seed
Effect assessment：
From above-mentioned table 1, by sprouting treated its germination percentage of seed of preceding seed treatment liquid apparently higher than comparative example 1
In the seed without the processing of seed treatment liquid, wherein, the percentage of seedgermination after the processing of potassium nitrate solution that concentration is 1% is
72.67%, 20.67% is higher by than comparative example 1, the percentage of seedgermination after the gibberellin leaching solution processing that concentration is 100mg/L is
82%, improve 30% than comparative example 1.
Embodiment 4
One day mid or late April is selected, seed sowing is carried out on the soil of fertile soft, gas permeability and good water-retaining property, is broadcast
Kinds first 3 days, by soil deep tillage 25cm, open railway carriage or compartment ditching, the long 10m in railway carriage or compartment, wide 1m, ditch depth 20cm, and it is equal with 0.1% liquor potassic permanganate
Even to splash in nursery bed disinfection soil, sowing irrigates seedbed in first 2 hours with clear water；
1 is pressed using three groups of red gentian seeds after the method seed soaking of embodiment 1 with plant ash respectively:500 it is mixed
Composition and division in a proportion example is mixed thoroughly, is uniformly sowed on seedbed, seed application rate is 50g/ mus.It is soil moisture content to sprout the environmental requirement emerged
For 50%, earth's surface air humidity is 60%, and temperature is 20 DEG C, and builds sunshade net, and the degree of shading of sunshade net is 70%.According to soil
Earth humidity, temperature, weather condition are watered in good time, keep ground moistening, carry out the experiment of emerging of 100 days by a definite date.Periodically remove miscellaneous
Grass, insect prevention, timing observation, record.
Embodiment 5
One day mid or late April is selected, seed sowing is carried out on the soil of fertile soft, gas permeability and good water-retaining property, is broadcast
Kinds first 3 days, by soil deep tillage 25cm, open railway carriage or compartment ditching, the long 10m in railway carriage or compartment, wide 1m, ditch depth 20cm, and it is equal with 0.1% liquor potassic permanganate
Even to splash in nursery bed disinfection soil, sowing irrigates seedbed in first 2 hours with clear water；
Using second group after the method seed soaking of embodiment 3, the 3rd group and the 4th group of red gentian seed respectively with
Plant ash presses 1:500 mixed proportion is mixed thoroughly, is uniformly sowed on seedbed, seed application rate is 50g/ mus.Sprout the ring emerged
It is that soil moisture content is 50% that border, which is required, and earth's surface air humidity is 60%, and temperature is 20 DEG C, and builds sunshade net, sunshade net
Degree of shading is 70%.Watered in good time according to soil moisture, temperature, weather condition, keep ground moistening, carried out 100 days by a definite date
Emerge experiment.Periodically remove weeds, insect prevention, timing observation, record.
Comparative example 2
One day mid or late April is selected, seed sowing is carried out on the soil of fertile soft, gas permeability and good water-retaining property, is broadcast
Kinds first 3 days, by soil deep tillage 25cm, open railway carriage or compartment ditching, the long 10m in railway carriage or compartment, wide 1m, ditch depth 20cm, and it is equal with 0.1% liquor potassic permanganate
Even to splash in nursery bed disinfection soil, sowing irrigates seedbed in first 2 hours with clear water；
1 is pressed using the red gentian seed after the method seed soaking of comparative example 1 and plant ash:500 mixed proportion is mixed
It is even, uniformly sow on seedbed, seed application rate is 50g/ mus.It is that soil moisture content is 50% to sprout the environmental requirement emerged,
Earth's surface air humidity is 60%, and temperature is 20 DEG C, and builds sunshade net, and the degree of shading of sunshade net is 70%.According to soil moisture,
Temperature, weather condition are watered in good time, keep ground moistening, carry out the experiment of emerging of 100 days by a definite date.Weeds, insect prevention are periodically removed,
Timing observation, record.
Effect example 2
For show method that the present invention promotes red gentian seed to sprout have rapidly and efficiently abolish red gentian seed
Dormancy, improves percentage of seedgermination, it is possible to improve the technique effect of chitting piece emergence rate.
The germination method of special selection example 4 and embodiment 5 and the germination method of comparative example 2, emerge experiment for ground
Number of emerging and emergence rate emerge comparative evaluation, result of the test is as shown in table 2 below：
Table 2：Ground emergence rate experimental result
Effect assessment：
Seed after the germination obtained from above-mentioned table 2, the method for promoting red gentian seed to sprout using the present invention,
Its emergence rate apparently higher than seed not treated by the present method in comparative example 2, wherein, concentration for 1% potassium nitrate solution handle
Seed sprouting number afterwards is 183, than 73 plants of comparative example 2, is higher by 110 plants, after concentration is 100mg/L Gibberellins solution processing
Seeding ratio be 136 plants, than 73 plants of comparative example 2, be higher by 63 plants.From the above results, promoted using the present invention red
Seed after the germination that the method that flower rough gentian seed is sprouted is obtained, its emergence rate is apparently higher than the kind handled without the inventive method
Son.
Although illustrate and describing the present invention with specific embodiment, but it will be appreciated that without departing substantially from the present invention's
Many other changes and modification can be made in the case of spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (10)
1. a kind of method for promoting red gentian seed to sprout, it is characterised in that methods described comprises the following steps：
(1) seed treatment liquid is prepared：Prepare potassium nitrate, calcium chloride or Gibberellins solution and be used as seed treatment liquid；
(2) seed soaking before seed is sprouted：Red gentian seed gauze wrapped is placed in step (1) seed treatment liquid,
Soaked seed 20~30 hours in the environment of 18~22 DEG C, soaked seed 10~15 hours in the environment of being subsequently placed into 3~5 DEG C, then abandon leaching
Liquid is planted, wash seeds are clean, complete seed soaking；
(3) budding is sprouted：Seed after step (2) seed soaking is put into and is covered with the culture dish of moistening filter paper, is subsequently placed into
Cultivated 10~20 days in incubator, and keep filter paper to moisten, the red gentian seed after being germinateed.
2. the method for promoting red gentian seed to sprout as claimed in claim 1, it is characterised in that：Optionally carry out step
(4) sprout and emerge：Red gentian seed after step (2) seed soaking is mixed thoroughly with plant ash, seedbed is uniformly seeded in enterprising
Row sprouting is emerged 90~120 days, obtains red gentian seedling.
3. the method for promoting red gentian seed to sprout as claimed in claim 1, it is characterised in that potassium nitrate in step (1)
Solution quality percentage concentration is 1%~5%；
Preferably, calcium chloride solution mass percentage concentration is 0.25%~2% in step (1)；
Preferably, Gibberellins solution mass-volume concentration is 50mg/L~800mg/L in step (1).
4. the method for promoting red gentian seed to sprout as claimed in claim 1, it is characterised in that step (2) wash seeds
Method be with distilled water flushing 4~5 times, every time 3~8 minutes.
5. the method for promoting red gentian seed to sprout as claimed in claim 1, it is characterised in that step (3) incubator is
Illumination box, the cultivation temperature of the illumination box is 23~28 DEG C, and intensity of illumination is 2~4 grades, every illumination 12 in 12 hours
Hour is dark alternately.
6. the method for promoting red gentian seed to sprout as claimed in claim 2, it is characterised in that step (2) seed soaking
Red gentian seed and the mass ratio of plant ash afterwards is 1:500~1000.
7. the method for promoting red gentian seed to sprout as claimed in claim 2, it is characterised in that step (2) seed soaking
Red gentian seed afterwards is sowed first 2~4 days, is uniformly splashed in seedbed with 0.05~0.1% liquor potassic permanganate, to soil
Carry out disinfection, and irrigate seedbed with clear water within 2~3 hours prior to seeding.
8. the method for promoting red gentian seed to sprout as claimed in claim 2, it is characterised in that sprouting the environment emerged will
Ask as soil moisture content 45~65%, earth's surface air humidity 60~70%, 20~25 DEG C of temperature.
9. the method for promoting red gentian seed to sprout as claimed in claim 1, it is characterised in that methods described is specifically included
Following steps：
(1) seed treatment liquid is prepared：Prepare potassium nitrate, calcium chloride or Gibberellins solution and be used as seed treatment liquid；
The potassium nitrate solution mass percentage concentration is 1%, 2%, 3%, 4% and 5%；
The calcium chloride solution mass percentage concentration is 0.25%, 0.5%, 1%, 1.5% and 2%；
The Gibberellins solution mass-volume concentration is 50mg/L, 100mg/L, 200mg/L, 400mg/L and 800mg/L；
(2) seed soaking before seed is sprouted：By red gentian seed with 2~3 layers of gauze wrapped, step (1) seed treatment is placed in
In liquid, soak seed 20~30 hours, soaked seed 10~15 hours in the environment of being subsequently placed into 3~5 DEG C, so in the environment of 18~22 DEG C
After abandon seed-soaking liquid, by seed distilled water flushing 4~5 times, 3~8 minutes every time, complete seed soaking；
(3) budding is sprouted：Seed after step (2) seed soaking is put into and is covered with the culture dish of 2~3 layers of moistening filter paper, with
After be put into illumination box, be 23~28 DEG C in temperature, intensity of illumination is 2~4 grades, every illumination in 12 hours 12 hours is dark to hand over
For culture 10~20 days, and filter paper is kept to moisten, the red gentian seed after being germinateed；
Optionally carry out,
(4) sprout and emerge：By the red gentian seed after step (2) seed soaking and plant ash with 1:500~1000 quality
Than mixing thoroughly, uniformly it is seeded in progress sprouting on seedbed and emerges 90~120 days, it is soil moisture content 45 to sprout the environmental requirement emerged
~65%, earth's surface air humidity 60~70%, 20~25 DEG C of temperature obtains red gentian seedling.
10. a kind of red gentian seedling, it is characterised in that the red gentian seedling is as described in any one of claim 1~9
The method for promoting red gentian seed to sprout is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611009376.4A CN107027372A (en) | 2016-11-16 | 2016-11-16 | A kind of method for promoting red gentian seed to sprout and its seedling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611009376.4A CN107027372A (en) | 2016-11-16 | 2016-11-16 | A kind of method for promoting red gentian seed to sprout and its seedling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107027372A true CN107027372A (en) | 2017-08-11 |
Family
ID=59531002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611009376.4A Pending CN107027372A (en) | 2016-11-16 | 2016-11-16 | A kind of method for promoting red gentian seed to sprout and its seedling |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107027372A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106717260A (en) * | 2016-11-29 | 2017-05-31 | 青岛松良基因科技有限公司 | The method for culturing seedlings of flower of Radix Gentianae and the implantation methods of flower of Radix Gentianae |
| CN108934806A (en) * | 2018-05-31 | 2018-12-07 | 界首市嘉涛农业发展有限公司 | A kind of sowing implantation methods improving rough gentian medical value |
| CN110447338A (en) * | 2019-09-09 | 2019-11-15 | 中国科学院昆明植物研究所 | A method of the Gentianaceae seed after promoting storage is sprouted |
| CN110679455A (en) * | 2019-09-26 | 2020-01-14 | 江西省林业科学院 | Water planting method for solanum nigrum |
| CN113288933A (en) * | 2020-02-22 | 2021-08-24 | 邓学峰 | Preparation formula and preparation method of honey-fried safflower gentian decoction pieces |
| CN114097543A (en) * | 2021-11-26 | 2022-03-01 | 锡林郭勒盟蒙医医院(锡林郭勒盟蒙医研究所、锡林浩特市人民医院) | Introduction and domestication cultivation method and application of gentianella acuta |
-
2016
- 2016-11-16 CN CN201611009376.4A patent/CN107027372A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106717260A (en) * | 2016-11-29 | 2017-05-31 | 青岛松良基因科技有限公司 | The method for culturing seedlings of flower of Radix Gentianae and the implantation methods of flower of Radix Gentianae |
| CN108934806A (en) * | 2018-05-31 | 2018-12-07 | 界首市嘉涛农业发展有限公司 | A kind of sowing implantation methods improving rough gentian medical value |
| CN110447338A (en) * | 2019-09-09 | 2019-11-15 | 中国科学院昆明植物研究所 | A method of the Gentianaceae seed after promoting storage is sprouted |
| CN110679455A (en) * | 2019-09-26 | 2020-01-14 | 江西省林业科学院 | Water planting method for solanum nigrum |
| CN113288933A (en) * | 2020-02-22 | 2021-08-24 | 邓学峰 | Preparation formula and preparation method of honey-fried safflower gentian decoction pieces |
| CN114097543A (en) * | 2021-11-26 | 2022-03-01 | 锡林郭勒盟蒙医医院(锡林郭勒盟蒙医研究所、锡林浩特市人民医院) | Introduction and domestication cultivation method and application of gentianella acuta |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107027372A (en) | A kind of method for promoting red gentian seed to sprout and its seedling | |
| CN105794354B (en) | A kind of sweet photo seed float seedling method | |
| CN104429581B (en) | A kind of Organic tomato health cultural method | |
| CN103477858A (en) | Cherry tomato planting method | |
| CN106106004A (en) | A kind of rapid cuttage propagation method of Lagerstroemia indica L. individual plant kind matter | |
| CN102919035A (en) | Industrialized cultivation method of sweet-scented osmanthus nursery stock | |
| CN101822165A (en) | Method for increasing seedling rate of hybrid pineapple seeds | |
| CN107926715A (en) | A kind of eggplant or/and the engrafting and cultivating method of capsicum or/and tomato | |
| CN104620921A (en) | Rapid propagation method for jujubes | |
| CN103039366B (en) | Industrial production method of mycorrhizal seedlings of Changbai Mountain rhododendron plants | |
| CN109566244A (en) | A kind of oil tea implantation methods | |
| CN108575626A (en) | The implantation methods of sealwort | |
| CN107821074A (en) | A kind of implantation methods for shortening leek breeding time | |
| CN107258267A (en) | A kind of implantation methods of plastic tent cucumber | |
| CN107306641A (en) | A kind of implantation methods of balsam pear | |
| CN106900326A (en) | A kind of fast breeding, the implantation methods of high yield strawberry | |
| CN106888948A (en) | A kind of seed propagation method of first hat rattan | |
| CN104871778A (en) | Cape gooseberry seedling cultivation method | |
| CN106431656A (en) | Sowing and seedling culture method of pittosporum tobira | |
| CN107996325B (en) | Method for preventing and controlling sorghum head smut by floating plate seedling culture | |
| CN110199698A (en) | A kind of method of greenhouse balsam pear graft seedling growth | |
| CN110214614A (en) | A kind of implantation methods of cinnamomum camphora tree | |
| CN107006313A (en) | A kind of rice seedlings cultivating method | |
| CN106561367A (en) | Paddy rice mixed sowing and mechanical transplanting cultivation method | |
| CN112889613A (en) | Planting method for improving cold resistance of cowpea in seedling stage |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170811 |
|
| RJ01 | Rejection of invention patent application after publication |