CN108207167A - The method of Tomato Seeds Germination rate and observation device under a kind of raising drought stress - Google Patents
The method of Tomato Seeds Germination rate and observation device under a kind of raising drought stress Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The present invention provides a kind of methods for improving Tomato Seeds Germination rate under drought stress, handle tomato seeds using 8 Br cGMP of suitable concentration, improve germination rate of the tomato seeds under drought stress.In addition, the present invention also provides a kind of seeds for simulating natural light inductive condition to sprout observation device, comparison and its morphologic observation in Tomato Seeds Germination experiment are realized.8 Br cGMP used in this experimental method have membrane permeability, and utilization rate is high, and 8 Br cGMP of low concentration can reach the significant germination rate for improving tomato seeds under drought stress.The experimental rig that this experiment is taken is simple in structure, easy to operate and repeated strong, can be observed different seed sprouting situations in real time, in addition, device setting processing and repetition are more flexible.
Description
Technical field
The present invention relates to plant physiology research field, Tomato Seeds Germination under specifically a kind of raising drought stress
The method of rate and mating observation device.
Background technology
In recent years, bio-hormone and signaling molecule to plant the seed under stress sprout and growth and development in terms of research get over
Paid attention to get over, especially in signal transduction and the degeneration-resistant aspect of biology.Six generally acknowledged big bio-hormones：Auxin（IAA）, gibberellin
（GA）, the basic element of cell division（CTK）, abscisic acid（ABA）, ethylene（ETH）With rape element sterol（BR）And signaling molecule salicylic acid
（SA）, nitric oxide（NO）, carbon monoxide（CO）Deng by successively applied to the degeneration-resistant research of plant.Abundant experimental results show
Micro plant hormone and small molecule semiochemicals is the key factor for adjusting seed dormancy, sprouting, and in the degeneration-resistant life of plant
Positive effect is played in long development.As research shows that, suitable concentration H2O2Seed is handled as a kind of adverse circumstance signaling molecule, it can
Promote the germination rate of eggplant, soybean low temperature is promoted to sprout, induce activities of antioxidant enzymes enhancing in plant, alleviate unfavorable conditions and draw
The oxidative stress risen；H2S pre-processes wheat seed as a kind of active Redox molecules, NaHS as donor, delays
Solve the inhibition of salt and Acid-Al stress to Seed Germination hydrolase, it will be apparent that induced activation is so as to ensure that effectively sprouting for seed
Hair；The horizontal GA of various concentration3Paeonia papaveracea seed is handled, there can be significant facilitation to taking root for seed, show as low rush
The dependence effect of height suppression；Ma Tianyuan et al. study SA under drought stress Seeds Germination of Coronilla Varia the result shows that, addition it is suitable
The SA of concentration can promote seed to sprout, shoot growth, and slow down injury of the drought stress to plant cell membrane.Except this, SA soaks
Kind is sprouted by Nyssa yunnanensis seed under drought stress and early stage growth of seedling has significant facilitation.
Cherry and tomato（Lycopersicon esculentum Mill）It is to belong to the annual of Solanaceae tomato genus or for many years
Raw herbaceous plant also known as cherry tomato, pearl fruit, little tomato.Because it contains a variety of lifes such as abundant vitamin and lycopene
Active substances, have promote the production of body fluid to quench thirst, appetite-stimulating and indigestion-relieving, hypertension and hyperlipemia, unique oxidation resistance and anti-cancer and prevention of cardiovascular
Disease and other effects, and favored deeply by masses.In recent years, with the raising of people's quality of life and the change of dietary structure, tomato
It is all good rather fashionable in the international market with its quality and flavor, it is the primary raw material of cold vegetable dish in sauce and postprandial high-grade fruit, therefore
Its demand is also equally more and more with melon and fruit sugar vegetable, is also to increase year by year along with cultivated area.However, as environmental pollution
The multi-panels factor such as the not science of unreasonable irrigation and chemical fertilizer application, leads to covered soil in aggravation and agricultural production
Dehydration is in aggravation trend, so as to bring huge problem directly to Tomato Seeds Germination and nursery and its planting.Therefore to release
Or the dehydration alleviated caused by drought stress wanes with Ion toxicity by interfering physiology course Tomato Seeds Germination and life
Long development is by serious inhibition, and to avoid cost of idleness, underproduction decrement, seed is sprouted into primary research center of gravity, so dress
It puts and comes into being.This seed sprouts observation device with production is not subject to seasonal restrictions, the period is short, simple to operation, at low cost, easy
Confirmatory test phenomenon is stayed in observing and can take pictures, more expensive chemical reagent can be more saved and reduce cost, can also be trained by screening
Required seedling is educated, the advantages that reproduction speed is fast, and transplanting survival rate is high.Using the method for presoaking and germinating, stringent control condition is being planted
Son is sprouted in slot through the Solution culture method of each processing, is sprouted and is grown to its seed, and observation experiment easily and effectively is existing
As, and the phenotypic difference analyzed between each processing that energy is considerable, for the seed sprouting good and the bad under effectively alleviation or relief of drought stress not
Together, the low problem of germination rate provides excellent testing equipment, and can the Resistence research of growth and development provides under adverse circumstance for plant
Certain guarantee and basis.
Problem of the existing technology：
Plant under abiotic stress has differences in ontogenetic different phase, and Their Seed Germinating Period is very quick to drought stress
Sense, the characteristic in this period determine whether the plant can successfully build seedling in a certain area.Therefore, can seed keep living
Can power, normally sprout and seedling growth is precondition that can plant survive under height drought environment.Because of cherry
One layer of tomato seeds surface epidermal hair, and its kind of skin is thicker, it is to arid sensitive crop, the side of body that seed, which sprouts slow tomato seeds,
Compel caused dehydration to wane with Ion toxicity by the way that its Seed Germination and growth of physiology course is interfered to develop by serious
Inhibition, so as to cost of idleness, the underproduction be reduced.Processing has improved report that cherry and tomato seed is sprouted also under existing adverse circumstance environment
Much, but it can only mitigate the inhibition sprouted under stress to seed, and recovery extent is not very superior, and is ground under drought stress
Study carefully also seldom.Therefore using cherry and tomato as material in the present invention, it is intended to which external source applies 8- bromine cyclic guanylic acids under arid situation（8-Br-
cGMP）Seed is handled, probes into the more excellent breeding apparatus and its method that can improve cherry and tomato seed germination rate.
Although external source adds hormone and signaling molecule and other substances has numerous studies at the degeneration-resistant aspect of plant,
In Tomato Seeds Germination, particularly under Abiotic stress conditions（Especially drought stress）Research it is very few.And research shows that,
The hormone or signaling molecule and the lower tomato seeds of other substances processing stress of external source addition suitable concentration can promote its seed to sprout
And its growth and development, but its recovery extent is limited.Tomato Seeds Germination often suffers from arid inhibition, and root system of plant is experienced earliest
Environment stress signal, and relevant physiological reaction is generated, then influence aerial growth.In prior art research, as a result table
The bright root system to tomato under drought stress is established, and the growth of root hair and its induction of lateral root development have not been reported.
Existing seed sprouting device is more lengthy and jumbled, and simple but workload is larger, and device poor air permeability causes the mold of seed, increases
Big experiment error, and the growing space after the sprouting of limitation seed, and effectively cannot intuitively observe seed and sprout to a certain extent
The form of hair.For this purpose, the present invention also provides a kind of seeds for simulating natural light inductive condition to sprout observation device, which can be with
More excellent growing environment is provided for seed sprouting, more suits current conditions.Also it can be saved for experimenter unnecessary, cumbersome
Repetitive operation is saved the time, observes effectively and intuitively experimental phenomena, and provide thinking for next step scientific research.
Invention content
In view of the deficiencies in the prior art, the present invention provides a kind of sides for improving Tomato Seeds Germination rate under drought stress
Method handles tomato seeds using the 8-Br-cGMP of suitable concentration, improves germination rate of the tomato seeds under drought stress.In addition,
The present invention also provides a kind of seeds for simulating natural light inductive condition to sprout observation device, realizes in Tomato Seeds Germination experiment
Comparison and its morphologic observation.
In order to achieve the above object, present invention employs following technical solutions：
The method of Tomato Seeds Germination rate, includes the following steps under a kind of raising drought stress：
（1）The arid concentration screening of tolerance
PEG-6000 Drought stress simulations, setting concentration is respectively 0 (CK), and 5%, 10%, 15%, 20%, tomato seeds are handled respectively,
Observe its germinating, the results showed that, 5%PEG processing cherry and tomato seeds show as the slight facilitation of low arid, and
Increase depression effect with PEG concentration to aggravate, 15%PEG is half lethal concentration, sprouts after processing cherry and tomato seed and is seriously pressed down
System, and 20% almost inhibits sprouting.Therefore, the Tomato Seeds Germination of 10% PEG processing is more considerable；In follow-up test
10% PEG is chosen as experiment stress concentration.
（2）8-Br-cGMP concentration is set
If a concentration of 0 (CK), the 8-Br-cGMP processing cherry and tomato seeds of 20 μm of ol/L and 40 μm of ol/L see its sprouting.Knot
Fruit shows that external source addition 8-Br-cGMP can improve seed germination rate.20 μm of ol/L 8-Br-cGMP and 40 μm of ol/L 8-Br-
CGMP treatment effects have Tomato Seeds Germination and growth slight facilitation, 20 and 40 μm of ol/L 8-Br- compared with CK
It is compared between cGMP, then more preferably, main root is grown faster resultant effect, more healthy and strong after 20 μm of ol/L 8-Br-cGMP processing, and
Well developed root system, lateral root is more, shows as low concentration and promotes effect.To study under drought stress conditions, 20 μm of ol/L and 40 μ
It is as under normal circumstances or low whether influences of the mol/L 8-Br-cGMP to Tomato Seeds Germination serves
Concentration has any similarities and differences again with tomato seeds, the effect between sprouting under higher concentration drug processing drought stress, therefore follow-up
Experiment is chosen 20 μm of ol/L 8-Br-cGMP and 40 μm of ol/L 8-Br-cGMP and is mutually dealt with for drug concentration for the treatment of and arid
Tomato species.
（3）8-Br-cGMP processing
20 μm of ol/L 8-Br-cGMP of setting processing, 40 μm of ol/L 8-Br-cGMP, 10% PEG, 10% PEG+20 μm of ol/L
8-Br-cGMP, 10% PEG+40 μm of ol/L 8-Br-cGMP, to distill water process as control（CK）.Select it is healthy and strong, full,
Consistent cherry and tomato seed uniform in size is impregnated for 24 hours with each treatment fluid 20ml is dark at normal temperatures after sterilized, then distilled
Water washing blots, its kind is sprouted in seed in device, is separately added into a concentration of CK, 20 μm of ol/L 8-Br-cGMP, 40 μ
mol/L 8-Br-cGMP、10% PEG、10% PEG+20μmol/L 8-Br-cGMP、10% PEG+40 μmol/L 8-Br-
The treatment fluid of cGMP places it in 26 DEG C ± 1 of illumination box light dark（16h/8h）Culture.Rudiment is dashed forward with radicle and broken in the seed coat
Length be seed size in itself 2 times be mark, daily count chitting piece number, 7d terminate sprout experiment, calculate germinating energy, hair
Bud rate, germination index and vitality index and its radicle length, plumular axis length and single-strain fresh weight amount, and analysis gained is handled with corresponding software
Data.
（4）Sprout effect assessment
It is evaluated using following index of sprouting：
Germinating energy（%）=（After planting 4d germinative numbers/seed sum）×100%；
Germination percentage（%）=（After planting 7d chitting pieces number/seed sum）×100%；
Germination index=Σ（Day by day germinative number germination test number of days）；
Plumular axis length（cm）；
Radicle length（cm）；
Single-strain fresh weight（mg）；
Vitality index=single plant Fresh Yuxincao × germination index；
Measuring instrument includes used in These parameters：Vernier caliper, assay balance.
The present invention also provides a kind of Tomato Seeds Germinations to observe device, and structure includes：Seed sprouts slot, and seed sprouts slot
Sieve is placed in inside, and seed sprouts slot underside and is connected with hole graded tube, has and filter paper is placed in the graded tube of hole；There is hole graded tube
It is placed on hose rack to fix, there is hole graded tube to be placed in glass guide channel；Partition board is placed in glass guide channel；Nut and standing screw are logical
Rubber sleeve is crossed to assemble seed sprouting observation device.
Preferably, it is funnel-form that seed, which sprouts groove shape, is for larger area ingress of air, ensures the ventilative of device
Property.
Preferably, filter paper is rolled into arc-shaped and has been placed in the graded tube of hole, occupy hole graded tube perimeter 2/3rds
Point, expose scale.
Preferably, glass guide channel and plastic septum are all made of the excellent material of light transmission.
Preferably, the graded tube with holes is equipped with scale and transparent, can quickly read the length of root after seed is sprouted
And growthform.
Preferably, the scale bore with holes is 3-6mm, and high 15-20cm is fixed on hose rack in glass guide channel；Hold
Product is big, can hold enough culture solutions, and avoid the unnecessary waste of resource.
Preferably, seed sprout slot on enclose diameter 1-2cm, under enclose diameter 2.1-5.2mm；Sieve diameter 2.5-5.5mm and
Aperture on hose is all that good air circulation provides safeguard；Plastic dam, can existence or non-existence as needed, if
Processing is more, repeats less, partition board septal area can be plugged into multiple culture solution containing tanks, with preferably for research effect.
Preferably, having on the scale tube wall of hole has aperture, diameter 1mm or so, by a definite date good air permeability, and places filter paper rainbow
Inhale it is ineffective lead to drying, influence seed sprouting.
Advantageous effect：
This test method effectively relieves the inhibition that cherry and tomato seed is sprouted under drought stress, not only shortens it and sprouts week
Phase, it is low more to solve germination rate, sprouts very different, and the various disadvantages such as slow-growing greatly enhance cherry and tomato
Germination percentage, germinating energy, germination index and the vitality index of seed, and high quality, the strong sprout of high quantity can be cultivated for building seedling
Plantation.8-Br-cGMP used in this experimental method has membrane permeability, and utilization rate is high, and low concentration 8-Br-cGMP can reach significant
Improve the germination rate of tomato seeds under drought stress.
It is more superior that the experimental rig that this experiment is taken relatively has seed sprouting observation device.It is embodied in：First,
The apparatus structure is simple, and easy to operate and repeated strong, addition treatment fluid is convenient and avoids unnecessary waste, device gas permeability
Well, growing space is enough, is sprouted for seed and to grow the environmental condition that provides more reasonable；Secondly, which can carry out reality
When observe, see the variation similarities and differences between its processing and its between repeating, and it is capable of taking pictures stay card, it is direct strong to illustrate experimental phenomena and effect
Fruit；Finally, which, which can apply, observes various seed sprouting situations, and setting processing and repetition are more flexible, can be according to implementation
Depending on person's demand.Multifactor impact is avoided, the interdisciplinary science research being more affixed under identical conditions between different disposal.Moreover, place
Reason observation seed is sprouted other than this characteristic, is applied also for being difficult to seedling, expensive plant nursery a little, be cultivated strong
Seedling is to be more advantageous to building seedling and breeding seed.
Seed Germination Test is observed in the method for presoaking and germinating under the conditions of present invention simulation natural light, utilizes controllable environment
Condition is to observe in Seed Germination Test, and being dashed forward using radical bud when breaking in the seed coat growth more than seed twice, length is mark, and observation is everywhere
The formation and growth of root between reason provide convenient and reliable observation device for plant seed germination experiment, assist and given birth in plant adverse circumstance
The Resistence research of reason；Meanwhile can screen and cultivate a small amount of special or expensive and be difficult to the plant seedlings survived, it is bred for planting strong sprout
Seed provides approach.So on the one hand device can be used for scientific research, actual production on the one hand can also be used for.Its advantage exists
In, for Seed Germination Test research provide a kind of new mode, can solve directly observe root in Seed Germination
The problems such as limited space of long and seminal root gravitropism growth；And setting considers the gas permeability in germination process, very great Cheng
Seed is avoided on degree, and immersion mouldy the problem of festering, accuracy for experiment due to poor air permeability provides certain for a long time
Ensure.This programme can realize the comparative observation that seed is sprouted between different disposal, more concentrate during scientific research is carried out,
Multifactor impact is avoided, the interdisciplinary science research being more affixed under identical conditions between different disposal.
Description of the drawings
Fig. 1 is that the method for the present invention seed dynamics sprout number result displaying figure.
Fig. 2 is each index result displaying figure of the method for the present invention Their Seed Germinating Period.
Fig. 3 is the front view that seed of the present invention sprouts device.
Fig. 4 is the right view that seed of the present invention sprouts device.
Fig. 5 is the partial structural diagram that seed of the present invention sprouts device.
Wherein, 1- seeds, which sprout slot, 2- sieves, 3- filter paper, 4-, free graded tube, 5- glass guide channels, 6- hose racks, 7- plastics
Partition board, 8- nuts, 9- standing screws, 10- rubber sleeves, 11- seeds.
Specific embodiment
Purpose, technical scheme and advantage to make invention are clearer, below in conjunction with the accompanying drawings to the specific implementation of the present invention
Mode is described in detail.The example of these preferred embodiments is illustrated in the accompanying drawings.Shown in attached drawing and according to attached
The embodiments of the present invention of figure description are only exemplary, and the present invention is not limited to these embodiments.
Here, it should also be noted that, in order to avoid because having obscured technical scheme of the present invention during unnecessary details,
It illustrate only the structure and/or processing step closely related with scheme according to the present invention in attached drawing, and relationship be omitted not
Big other details.
Embodiment 1
The present embodiment provides a kind of methods for improving Tomato Seeds Germination rate under drought stress, include the following steps：
（1）The arid concentration screening of tolerance
Using PEG-6000 Drought stress simulations.Experiment setting processing liquid concentration is respectively 0 (CK), 5%, 10%, 15%, 20%
PEG-6000.It cleans and assembles seed sprouting observation device and be dried for standby.Full, uniform cherry and tomato seed is selected,
With each treatment fluid 20ml, dark seed soaking for 24 hours, is then distilled water washing three times and is blotted at normal temperatures after sterilized, and radicle sprouts position
It puts and is positioned over screen position two in seed sprouting observation device seed sprouting slot downward, placement 40 is so repeated, in seed
The treatment fluid 1 that its volume half is injected in observation device glass guide channel is sprouted, i.e. distilled water is control（CK）；Seed sprouts observation
Device second row is similarly put into the cherry and tomato seed of 40 selects, with its volume two in liquid-transfering gun implantation glass slot/
One treatment fluid 2, i.e., 5% PEG solution；Other three kinds processing are ibid described in processing 1,2.After handling tomato seeds respectively, place
Regulating and controlling light intensity for 12000Lx, light application time 16/8h illumination/dark, 26 ± 1 DEG C of temperature, the illumination cultivation of relative humidity 80%
It is cultivated in case, added in respective handling liquid every three days, observe its germinating daily, taken pictures and see that its Root Morphology changes, and directly
To there is scale on the graded tube of hole to read its root long degree.Dashed forward using radicle break in the seed coat length as seed itself size 2 be mark again, daily
Statistics germination number, germinating energy, germination percentage, germination index are calculated with Excel2010 according to respective formula；And terminate in experiment
Last day weighs single-strain fresh weight, calculates vitality index with above-mentioned same method, as a result concludes table with three line tables and block diagram
Show, and analyze difference between its processing.The result shows that 5%PEG processing cherry and tomato seeds show as the slight promotion of low arid
Effect, and increase depression effect with PEG concentration and aggravate, 15%PEG is half lethal concentration, and sprouting is tight after handling cherry and tomato seed
It is suppressed again, and 20% almost inhibits sprouting.Therefore, the Tomato Seeds Germination of 10%PEG processing is more considerable, subsequently
10%PEG is chosen in experiment as experiment stress concentration.
（2）8-Br-cGMP concentration is set
If a concentration of 0 (CK), 20 μm of ol/L, the 8-Br-cGMP processing cherry and tomato seeds of 40 μm of ol/L are seen under normal condition
Effect is whether there is to seed sprouting, how is effect degree, which aspect is in particular in, to making ratio under follow-up drought stress
Compared with method is same（1）It is described.The result shows that external source addition 8-Br-cGMP can improve seed germination rate, and its seedling is compared with CK more
It is healthy and strong.Compared between 8-Br-cGMP processing, 20 μm of ol/L 8-Br-cGMP and 40 μm of ol/L 8-Br-cGMP treatment effects compared with
CK has Tomato Seeds Germination and growth slight facilitation, phase between 20 μm of ol/L and 40 μm of ol/L 8-Br-cGMP
Than, then more preferably, main root is grown faster resultant effect, more healthy and strong, and well developed root system after 20 μm of ol/L 8-Br-cGMP processing,
Lateral root is more, shows as low concentration and promotes effect.To study under drought stress conditions, 20 μm of ol/L and 40 μm of ol/L 8-
Influences of the Br-cGMP to Tomato Seeds Germination whether serve as under normal circumstances or low concentration with compared with
Tomato seeds under high concentration drug processing drought stress, the effect between sprouting have any similarities and differences, are chosen in subsequent experimental again
20 μm of ol/L and 40 μm of ol/L 8-Br-cGMP mutually deal with tomato seeds for drug concentration for the treatment of and arid, observe its sprouting
Effect.
（3）8-Br-cGMP processing
20 μm of ol/L 8-Br-cGMP of setting processing, 20 μm of ol/L and 40 μm of ol/L 8-Br-cGMP, 10% PEG, 10%
PEG+20 μm of ol/L 8-Br-cGMP, 10% PEG+40 μm of ol/L 8-Br-cGMP, to distill water process as control（CK）.
Select healthy and strong, full, uniform in size each 40 of consistent cherry and tomato seed, it is sterilized after with each treatment fluid 20ml at normal temperatures
Dark seed soaking for 24 hours, is then distilled water washing and is blotted, as（1）The method is placed on seed and sprouts in device, in illumination
It is cultivated in incubator.Equally dashed forward using radicle break in the seed coat length as seed itself size 2 again be sprout mark, daily statistics hair
Bud seed number, and its Root Morphology is photographed to record, read root long.7d terminates to sprout experiment, calculates germinating energy, germination percentage, hair
Bud index and vitality index and single-strain fresh weight amount, as the above method handles data and is analyzed.
（4）Sprout effect assessment
It is evaluated using following index of sprouting：
Germinating energy（%）=（After planting 4d germinative numbers/seed sum）×100%；
Germination percentage（%）=（After planting chitting piece number/seed sum in 7d）×100%；
Germination index=Σ（Day by day germinative number germination test number of days）；
Vitality index=single plant Fresh Yuxincao × germination index；
Radicle is long：There are hole graded tube rod reading, unit（cm）；
Plumular axis is long：Vernier caliper measurement, unit（cm）；
Single-strain fresh weight：Assay balance, unit（mg）
The results are shown in Table 1, and 8-Br-cGMP significantly improves the processing method of cherry and tomato seed germination rate, and advantage is：10%
PEG+20 μm of ol/L 8-Br-cGMP solution treatment cherry and tomato seed significantly promotes its sprouting, and germinating energy reaches
58.3%, improve 51.46% than 10%PEG processing；Germination percentage reaches 85.8%, and 60.14% is improved than 10% PEG；Germination index
Reach 28.14,46.52% is improved than 10% PEG, compared with compareing germination index 29.88, it is right almost to relieve arid
Its inhibition is restored under normal condition.Vitality index reaches 324.53, improves 62.58% than 10% PEG, has surmounted control
The vitality index 281.39 of group.Nontoxic under 20 μm of ol/L 8-Br-cGMP low concentrations, dosage is few when handling seed, economical, the time
It is short, it is quick；Seed is injury-free, and method is simple, is not influenced by factors such as season, weather, natural calamities.
From attached drawing 1 it is found that germination period 2d, the sprouting of 1d cherry and tomato seeds of 10% PEG obvious postpones, and 10%
PEG+20 μm of ol/L 8-Br-cGMP and 10% PEG+40 μm of ol/L 8-Br-cGMP significantly relieve this inhibiting effect,
The lower interaction of stress significantly induces the sprouting for accelerating cherry and tomato seed, shortens sprouting number of days；It is accumulated with number of days is sprouted, and
Sprouting is effectively promoted, the lower seed of 10% PEG stress is sprouted slowly, and Some seeds is inhibited to sprout, ultimately fails to reach
It sprouts completely.And 10% PEG+20 μm of ol/L 8-Br-cGMP processing be obviously promoted seed sprout number, to 5d almost with
CK, 20 μm of ol/L and 40 μm of ol/L 8-Br-cGMP processing equally sprout seed almost all；And 10% PEG+20 μm of ol/
L 8-Br-cGMP are compared between 10% PEG+40 μm of ol/L 8-Br-cGMP processing, 10% PEG+20 μm of ol/L 8-Br-
For cGMP treatment effects more better than 10% PEG+40 μm of ol/L 8-Br-cGMP, show as low concentration is obviously promoted effect.
From attached drawing 2 it is found that 10% PEG significantly inhibit seed sprout after root in the growth of plumular axis, and 10% PEG+20 μ
The growth of main root and plumular axis is significantly promoted after mol/L 8-Br-cGMP processing, after being sprouted so as to side light to seed
Growth and development has significant positive effect；Single-strain fresh weight is as it can be seen that the stress of 10% PEG significantly reduces Seedling Biomass, shadow
The accumulation of its substance and growth and development are rung, and after 10% PEG+20 μm of ol/L 8-Br-cGMP processing, it will be apparent that it is fresh to promote seedling
Weight, improves seedling quality.It can solve the problems, such as that cherry and tomato seed germination rate is low with this method, be vegetables industrial crops
Biological study and cherry and tomato nursery production provide materials and methods device for the good germ plasm resource of germplasm, will also push
The production of other such as cherry and tomatos, human nutrition, root system development and growth and development of plants hormone regulating and controlling and signal transduction
The research of aspect pushes cherry and tomato nursery and its economic development of fruit.
1 8-Br-cGMP of table is to the influence to Tomato Seeds Germination index under drought stress
| Processing | Germinating energy | Germination percentage | Germination index | Vitality index |
| Ck | 0.687 | 0.875 | 29.882 | 281.386 |
| 20μmol/L | 0.767 | 0.9 | 31.454 | 589.762 |
| 40μmol/L | 0.733 | 0.875 | 31.017 | 563.484 |
| 10%PEG | 0.283 | 0.342 | 15.056 | 121.448 |
| 10%PEG+20μmol/L | 0.583 | 0.858 | 28.138 | 324.526 |
| 10%PEG+40μmol/L | 0.483 | 0.683 | 24.383 | 332.415 |
Embodiment 2
Tomato Seeds Germination observation device is present embodiments provided, as shown in attached drawing 3-4, structure includes：Seed sprouts slot 1,
Seed sprouts 1 inside of slot and places sieve 2, and seed sprouts 1 underside of slot and is connected with hole graded tube 4, has in hole graded tube 4 and place
Filter paper 3；There is hole graded tube 4 to be placed on hose rack 6 to fix, there is hole graded tube 4 to be placed in glass guide channel 5；Partition board 7 is placed on glass guide channel
In 5；Nut 8 and standing screw 9 are that seed is sprouted observation device by rubber sleeve 10 to assemble.
Further, it is funnel-form that seed, which sprouts 1 shape of slot, is for larger area ingress of air, ensures the saturating of device
Gas.
Further, filter paper 3 is rolled into arc-shaped and has been placed in hole graded tube 4, occupy 4 perimeter of hole graded tube three/
Scale is exposed in two parts.
Further, glass guide channel 5 and plastic septum 7 are all made of the excellent material of light transmission.
Further, the graded tube with holes 4 is equipped with scale and transparent, can quickly read the length of root after seed is sprouted
Degree and growthform.
Further, the scale bore with holes is 3-6mm, and high 15-20cm is fixed on hose rack in glass guide channel；
Volume is big, can hold enough culture solutions, and avoids the unnecessary waste of resource.
Further, seed sprout slot 1 on enclose diameter 1-2cm, under enclose diameter 2.1-5.2mm；Sieve diameter 2.5-
Aperture on 5.5mm and hose, is all that good air circulation provides safeguard；Plastic dam 7, can exist or not as needed
In the presence of if processing is more, repetition is few, partition board septal area can be plugged into multiple culture solution containing tanks, with preferably for research effect.
Further, having on 4 tube wall of hole graded tube has aperture, diameter 1mm or so, by a definite date good air permeability, and places filter
Paper siphon is ineffective to lead to drying, influences seed sprouting.
Embodiment 3
The present embodiment provides a kind of observation methods of Tomato Seeds Germination, as shown in figures 3-5, include the following steps：
（1）It cleans seed and sprouts slot 1, sieve 2 has hole graded tube 4, glass guide channel 5, hose rack 6 and glass partition 7, dries for use.
（2）According to specification summary and its attached drawing, all parts are assembled.Filter paper 3 is first rolled into arc-shaped and has been placed on hole
In graded tube 4, occupy 2/3rds parts of 4 perimeter of hole graded tube, expose scale.Sieve 2 has been layered on hole graded tube 4 to push up
Then seed sprouting slot 1 has been fixed on 4 top of hole graded tube by end；Then this assembling is positioned on hose rack 6, and hose
6 plug flat of frame enters 5 upside of glass guide channel and fixes, and glass guide channel 5 is put into rubber sleeve 10, finally will be multiple above-mentioned with nut 8 and standing screw 9
Structure assembling is fixed into a system（It is depended on the circumstances according to test process）.
（3）It selects after size is uniform, seed 11 of full appearance is washed and sterilized, by 11 plumule growth position of seed downward
It is positioned on sieve 2.
（4）Then it is needed culture solution adding in glass guide channel 5 according to experiment, adds in nutrient solution volume and about arrive 5 volume of glass guide channel
1/2 at；If experiment process is more, then needs to reduce repeating, glass partition 7 is inserted in glass guide channel 5 as needed, septal area shape
Into two slots, to reach experiment demand.
（5）Its whole device is finally positioned over temperature, humidity and its light application time and regulates and controls suitable illumination with luminous intensity
It is cultivated on incubator or illumination cultivation frame, appropriate time addition nutrient solution, the length that breaks in the seed coat of being dashed forward with radicle reaches 2 times of seed size
Indicate to sprout, observe and photograph to record experimental phenomena.
In conclusion the embodiment of the present invention 1 provides a kind of method for improving Tomato Seeds Germination rate under drought stress, have
Effect relieves the inhibition that cherry and tomato seed is sprouted under drought stress, not only shortens it and sprouts the period, more solves germination rate
It is low, very different, the various disadvantages such as slow-growing are sprouted, greatly enhance germination percentage, the germination of cherry and tomato seed
Gesture, germination index and vitality index, and high quality, the strong sprout of high quantity can be cultivated and planted for building seed.
2-3 of the embodiment of the present invention provides a kind of simulate and carries out seed under natural light controlled condition in the method for presoaking and germinating
The device and observation method of observation experiment are sprouted, intuitively can effectively observing seed, to sprout root leaf into and carrying out identical conditions
Under comparison between a variety of processing, provide foundation for research from phenotype.It can be tested using controllable photoinduction condition and improve kind
The degeneration-resistant border of son is sprouted, and Screening Treatment scheme cultivates a small amount of seedling, and improves its yield and quality, the strong sprout cultivated can planting with
Other researchs of phase or application.The present invention can be used for the various plant seed germination seedling of different sizes and root growth observation
Experimental provision or a small amount of nursery production equipment.
The above is only the specific embodiment of the application, it is noted that for the ordinary skill people of the art
For member, under the premise of the application principle is not departed from, several improvements and modifications can also be made, these improvements and modifications also should
It is considered as the protection domain of the application.
Claims (10)
- A kind of 1. method for improving Tomato Seeds Germination rate under drought stress, which is characterized in that it includes the following steps：（1）It is resistance to By arid concentration screening；（2）8-Br-cGMP concentration is set；（3）8-Br-cGMP processing；（4）Sprout effect assessment.
- A kind of 2. method for improving Tomato Seeds Germination rate under drought stress according to claim 1, which is characterized in that institute State step（1）The arid concentration screening of tolerance includes：PEG-6000 Drought stress simulations, setting concentration is respectively 0 (CK), and 5%, 10%, 15%, 20%, tomato seeds are handled respectively, observe its germinating, the results showed that, 5%PEG processing cherry and tomato kind sublists The now slight facilitation for low arid, and increase depression effect with PEG concentration and aggravate, 15%PEG is half lethal concentration, place It sprouts after reason cherry and tomato seed and is seriously suppressed, and 20% almost inhibits sprouting；Therefore, kind of 10% PEG processing Eggplant seed is sprouted more considerable；10% PEG is chosen in follow-up test as experiment stress concentration.
- A kind of 3. method for improving Tomato Seeds Germination rate under drought stress according to claim 1, which is characterized in that institute State step（2）Drug concentration setting includes：If a concentration of 0 (CK), the 8-Br-cGMP processing cherries of 20 μm of ol/L and 40 μm of ol/L Peach tomato seeds see its sprouting；The result shows that external source addition 8-Br-cGMP can improve seed germination rate, and its seedling is compared with CK more It is healthy and strong；It is compared between 8-Br-cGMP processing, 20 μm of ol/L 8-Br-cGMP treatment effects are better, show as low concentration rush Into effect；Therefore subsequent experimental chooses 20 μm of ol/L 8-Br-cGMP and is and arid interaction concentration for the treatment of.
- A kind of 4. method for improving Tomato Seeds Germination rate under drought stress according to claim 1, which is characterized in that institute State step（3）Drug-treated includes：20 μm of ol/L 8-Br-cGMP of setting processing, 10% PEG, 10% PEG+20 μm of ol/L 8- Br-cGMP, to distill water process as control（CK）；Healthy and strong, full, uniform in size consistent cherry and tomato seed is selected, is passed through It is impregnated for 24 hours after disinfection with each treatment fluid 20ml is dark at normal temperatures, then distills water washing and blot, its kind in seed is sprouted and is filled In putting, it is separately added into a concentration of CK, 20 μm of ol/L 8-Br-cGMP, 10% PEG, 10% PEG+20 μm of ol/L 8-Br-cGMP Treatment fluid, place it in 26 DEG C of illumination box light dark（16h/8h）Culture；Rudiment is dashed forward using radicle breaks in the seed coat length as kind Sub- size in itself 2 times for mark, daily count chitting piece number, 7d terminate sprout experiment, calculate germinating energy, germination percentage, hair Bud index and vitality index and its root long and single-strain fresh weight amount, and analysis the data obtained is handled with corresponding software.
- 5. a kind of Tomato Seeds Germination observes device, which is characterized in that its structure includes：Seed sprouts slot, and seed is sprouted in slot Sieve is placed in portion, and seed sprouts slot underside and is connected with hole graded tube, has and filter paper is placed in the graded tube of hole；There is hole graded tube to put It is fixed in hose rack, there is hole graded tube to be placed in glass guide channel；Partition board is placed in glass guide channel；Nut and standing screw are to pass through Seed is sprouted observation device and assembled by rubber sleeve.
- 6. a kind of Tomato Seeds Germination observation device according to claim 5, which is characterized in that seed sprouts groove shape and is Funnel-form is the gas permeability for ensureing device for larger area ingress of air；Filter paper is rolled into arc-shaped and has been placed on hole graded tube It is interior, occupy 2/3rds parts of hole graded tube perimeter, expose scale.
- 7. a kind of Tomato Seeds Germination observation device according to claim 5, which is characterized in that glass guide channel and plastic septum All it is made of the excellent material of light transmission；The graded tube with holes is equipped with scale and transparent, can quickly read seed The length and growthform of root after sprouting.
- 8. a kind of Tomato Seeds Germination observation device according to claim 5, which is characterized in that in the graded tube with holes Diameter is 3-6mm, and high 15-20cm is fixed on hose rack in glass guide channel；Volume is big, can hold enough culture solutions, and avoid The unnecessary waste of resource；Seed sprout slot on enclose diameter 1-2cm, under enclose diameter 2.1-5.2mm；Sieve diameter 2.5-5.5mm, And the aperture on hose, it is all that good air circulation provides safeguard；Having on the scale tube wall of hole has aperture, diameter 1mm or so, Good air permeability by a definite date, and place that filter paper siphon is ineffective leads to drying, influence seed sprouting.
- 9.8-Br-cGMP improves the application in plant seed germination rate under drought stress.
- 10. 8-Br-cGMP according to claim 9 improves the application in plant seed germination rate under drought stress, It is characterized in that, the vegetable seeds is tomato seeds.
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