CN115005034B - Device and method for identifying stress resistance of exogenous biological stimulus to rice seedling stage - Google Patents
Device and method for identifying stress resistance of exogenous biological stimulus to rice seedling stage Download PDFInfo
- Publication number
- CN115005034B CN115005034B CN202210665116.1A CN202210665116A CN115005034B CN 115005034 B CN115005034 B CN 115005034B CN 202210665116 A CN202210665116 A CN 202210665116A CN 115005034 B CN115005034 B CN 115005034B
- Authority
- CN
- China
- Prior art keywords
- rice
- stress
- box body
- biological
- root
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 61
- 235000009566 rice Nutrition 0.000 title claims abstract description 61
- 238000000034 method Methods 0.000 title claims abstract description 17
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 61
- 229940088597 hormone Drugs 0.000 claims abstract description 46
- 239000005556 hormone Substances 0.000 claims abstract description 46
- 230000000638 stimulation Effects 0.000 claims abstract description 31
- 229920000742 Cotton Polymers 0.000 claims abstract description 23
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 16
- 229920001223 polyethylene glycol Polymers 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000012530 fluid Substances 0.000 claims abstract description 12
- 230000000149 penetrating effect Effects 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 239000002202 Polyethylene glycol Substances 0.000 claims abstract description 6
- 230000035882 stress Effects 0.000 claims description 41
- 230000015784 hyperosmotic salinity response Effects 0.000 claims description 18
- 150000003839 salts Chemical class 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 12
- 241000196324 Embryophyta Species 0.000 claims description 10
- 239000004705 High-molecular-weight polyethylene Substances 0.000 claims description 10
- 230000024346 drought recovery Effects 0.000 claims description 10
- 210000004209 hair Anatomy 0.000 claims description 8
- 230000008641 drought stress Effects 0.000 claims description 7
- 238000011156 evaluation Methods 0.000 claims description 7
- 235000015097 nutrients Nutrition 0.000 claims description 7
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 6
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 claims description 6
- 229940093429 polyethylene glycol 6000 Drugs 0.000 claims description 6
- 239000012266 salt solution Substances 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 238000011161 development Methods 0.000 claims description 5
- 230000018109 developmental process Effects 0.000 claims description 5
- 239000012466 permeate Substances 0.000 claims description 5
- 230000004936 stimulating effect Effects 0.000 claims description 4
- 230000035784 germination Effects 0.000 claims description 3
- 239000000819 hypertonic solution Substances 0.000 claims description 3
- 229940021223 hypertonic solution Drugs 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 230000001502 supplementing effect Effects 0.000 claims description 3
- 210000002257 embryonic structure Anatomy 0.000 claims description 2
- 238000001704 evaporation Methods 0.000 claims description 2
- 238000009331 sowing Methods 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims 1
- 230000012010 growth Effects 0.000 abstract description 8
- 239000003513 alkali Substances 0.000 abstract description 4
- 230000002411 adverse Effects 0.000 abstract description 3
- 230000000877 morphologic effect Effects 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 description 8
- 230000008635 plant growth Effects 0.000 description 4
- 230000007547 defect Effects 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 239000007952 growth promoter Substances 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000001034 Frostbite Diseases 0.000 description 1
- 108010020346 Polyglutamic Acid Proteins 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- NCYVXEGFNDZQCU-UHFFFAOYSA-N nikethamide Chemical compound CCN(CC)C(=O)C1=CC=CN=C1 NCYVXEGFNDZQCU-UHFFFAOYSA-N 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000008654 plant damage Effects 0.000 description 1
- 229920002643 polyglutamic acid Polymers 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000010496 root system development Effects 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
- A01G13/02—Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
- A01G13/0243—Protective shelters for young plants, e.g. tubular sleeves
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G27/00—Self-acting watering devices, e.g. for flower-pots
- A01G27/04—Self-acting watering devices, e.g. for flower-pots using wicks or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/021—Pots formed in one piece; Materials used therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/027—Pots connected in horizontal rows
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Ecology (AREA)
- Engineering & Computer Science (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Forests & Forestry (AREA)
- Water Supply & Treatment (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Wood Science & Technology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention relates to the technical field of rice biological hormone identification, in particular to a method and a device for rapidly identifying exogenous biological hormone with stress resistance in a rice seedling stage; the transparent barrel is internally provided with a plurality of box bodies with equal volume, the bottom of each box body is provided with a water absorbing cotton layer, the top of each box body is provided with a black light-shading plate with a plurality of through holes and a plurality of opening field planting cotton, and the side wall of the transparent barrel at the bottom is provided with scale marks; the invention completes the seedling treatment under the same device, ensures the consistent experimental conditions, and avoids the error caused by the uneven concentration of the exogenous biological stimulation hormone liquid in the operation; the saline solution and polyethylene glycol penetrating fluid simulate the saline-alkali and drought stimulation under outdoor conditions, so that whether exogenous biostimulant has adverse resistance can be rapidly judged; the natural growth condition of the rice root system can be directly observed through the scale marks on the transparent box, and the morphological indexes of the overground part and the underground part are compared and observed.
Description
Technical Field
The invention relates to the technical field of rice biological hormone identification, in particular to a method and a device for rapidly identifying exogenous biological hormone with stress resistance in a rice seedling stage.
Background
The biological hormone is not pesticide, and is not traditional fertilizer. The target of the biological hormone is the crop itself, which can improve the physiological and biochemical states of plants, the pesticide effect and the fertilizer utilization rate, the low stress resistance level of the crop, and the final yield and the agricultural product quality of the crop. The natural biological hormone can improve the physiological and biochemical processes of crops, and stimulate and regulate the growth of crops. Experiments show that after one week of biostimulation, the leaves become green and thick, and the leaves grow to have texture and stand upright. Root hairs can be raised to 15% within 15 days, and the average yield of crops is increased by 10% -25%. The yield of the rhizome crops is increased by more than 60 percent; the plant growth promoter also has natural promoting effect on the root of crops, and can stimulate the regeneration of root hairs and the growth of root systems, thereby improving the nutrient absorption capacity of the root of crops, especially when the cold of the spring comes, the plant growth promoter can improve the activity of proline or antioxidant enzyme in plants and reduce the occurrence of frostbite by influencing the signal of calcium.
The area of the seawater rice in the coastal saline-alkali area in North Jiangsu is increased year by year, and the drought climate and the land salinization are already the main factors affecting the yield of the rice. Therefore, rapid screening and identification of biological hormones to enhance stress resistance of rice plants and breeding rice varieties with strong drought resistance and salt resistance have become important problems in the agricultural field.
The prior art has the following defects: 1. in the development of indoor experiments, the biological stimulation hormone is mostly added in the forms of foliar spraying, seed dressing, root irrigation and the like, the process of absorbing nutrition by plant roots is neglected, quantitative stimulation cannot be applied to the growth of plant seedlings, and visual observation is not facilitated; 2. most of the existing experiments are used for simulating stress to identify rice varieties, and screening of biological stimulation hormone is carried out under the condition of lacking stress; 3. the existing rice water planting device is mainly tested by adopting 96 pore plates, and the pore plates are small in size, so that the germination rate is low, and the growth is inhibited; the pore plate cannot be reused, so that resource waste is caused; 4. in the statistics of the results, the operation is troublesome, and the measurement needs to be taken out one by one. Removal of the plant damages the root system, resulting in inhibited or even stopped development. Therefore, we propose a rapid identification method for the growth promotion of exogenous biological stimulus A under rice seedling stage adversity.
Disclosure of Invention
The purpose of the invention is that: overcomes the defects in the prior art and provides a device for identifying the stress resistance of exogenous biological stimulation hormone to rice seedling stage.
The technical scheme adopted in the invention is as follows:
the device comprises a transparent barrel, a plurality of box bodies with equal volume are arranged in the transparent barrel, a water-absorbing cotton layer is arranged at the bottom of the box body, a black light-shading plate with a plurality of through holes and a plurality of opening field planting cotton are arranged at the top of the box body, and scale marks are arranged on the side wall of the transparent barrel at the bottom.
Further, the transparent barrel is a hard plastic barrel.
Another object of the invention is: overcomes the defects in the prior art and provides a method for identifying the stress resistance of exogenous biological stimulation hormone to rice seedling stage.
In order to achieve the above object, the present invention adopts the following technical scheme:
a method for identifying exogenous biostimulant for rice seedling stress resistance, said method comprising the steps of:
1) Uniformly sowing the sterilized rice seeds after being sterilized by hydrogen peroxide on absorbent cotton which fully absorbs water, taking a plurality of rice seedlings with basically uniform germination, fixing the rice seedlings on open field cotton (d=20mm) when the rice sprouts to 1-2cm, and placing seed embryos downwards; fixing the open field cotton on a porous black light-shading layer, so that a plurality of germinated seedlings are arranged in each hole of the light-shading layer for standby; taking out the absorbent cotton at the bottom of the box, adding water to fix the volume to the marked positions of the graduation marks of the transparent barrel; after the operation is finished, the box body is clamped on the transparent barrel according to the mark;
2) Preparing exogenous biological hormone, adding the biological hormone into the box body after the seedlings grow to a leaf and a core in the step 1), treating for a period of time, taking down the seedlings and the black light-resistant laminate after the stimulation is finished, and transferring the seedlings and the black light-resistant laminate into an empty box body;
3) Preparing and identifying salt solution and high-concentration high-molecular-weight polyethylene glycol penetrating fluid, and respectively adding the salt solution and the high-concentration high-molecular-weight polyethylene glycol penetrating fluid into a box body where seedlings are positioned, wherein the salt solution and the high-concentration high-molecular-weight polyethylene glycol penetrating fluid are used for simulating salt stress treatment and drought stress treatment;
4) And (3) identifying and evaluating salt tolerance and drought tolerance of the rice in the seedling stage.
Further, the concentration of the exogenous biological stimulus in the step 2) is 100-250mg/L, the treatment concentration is set to 3-5, and the obvious development difference trend of plant root systems and plant heights compared with ck is observed obviously after 5 days of treatment.
Further, the step 3) of the simulated salt stress treatment specifically comprises the following steps: preparing sodium chloride solution with mass concentration of 2-5%and respectively injecting into a box body where rice seeds treated by exogenous biological stimulation are located for 5 days, wherein a control group is equal amount of clear water, liquid in the box is gradually evaporated, hogland nutrient solution is supplemented every three days during stress until 14 days are finished, and seedlings are placed into a phytotron for culture.
Further, the simulated drought stress treatment in the step 3) specifically comprises the following steps: preparing 67, 100 and 200g/L high-concentration high-molecular-weight polyethylene glycol penetrating fluid, respectively injecting the high-concentration high-molecular-weight polyethylene glycol penetrating fluid into a box body where rice seeds treated by exogenous biological stimulation are located for 5 days, gradually evaporating the liquid in the box body by using equal amount of clear water as a control group, supplementing Hogland nutrient fluid once every three days during stress until the treatment is completed, and placing seedlings into a climatic incubator for culture.
Further, the polyethylene glycol permeate in the step 3) is polyethylene glycol-6000 hypertonic solution.
Further, the temperature in the artificial climate incubator is 25 ℃, the relative humidity is 80% +/-2%, and the light intensity is 1600lx.
Further, the step 4) specifically includes the following steps:
a. the rice seedlings are taken out after being stressed in a climatic chamber at 25-30 ℃ for 14 days, the root length of the lower part of the box body is investigated and counted, and the transparent box body is marked with numbers 1-10, so that the index of the root system can be directly read out;
b. taking root bud index, root hair number, salt tolerance coefficient and drought tolerance coefficient as indexes for evaluating the excellent biological stimulation; counting and investigating the area of the upper yellow leaf and the number of root hairs by using a root system scanner; based on the measured values of the indices, an average value is calculated, and the individual items are calculated according to the following formula:
root bud index = rice seedling height/root length
Salt tolerance coefficient = relative Huang Shebi (number of yellow leaves per total number of leaves of treatment group) ×sodium chloride solution concentration×1000
Salt tolerance coefficient = relative Huang Shebi (number of yellow leaves per total number of leaves of treatment group) ×polyethylene glycol-6000 mass concentration/100
c. The evaluation was made according to root bud index on the following 3 scale:
(1) high stress-resistant biological hormone root bud coefficient >1.2
(2) 1.0< medium stress resistance organism stimulating hormone root bud coefficient <1.2
(3) Stress-free biological hormone root bud coefficient is less than 1.0
d. Evaluation was made according to the salt (drought) tolerance coefficient as registered below:
(1) high stress-resistant biological hormone salt tolerance (drought) coefficient <1
(2) The stress-free biological stimulation hormone salt tolerance (drought) coefficient is more than 1.
The technical scheme of the invention has the beneficial effects that:
the invention completes the seedling treatment under the same device, ensures the consistent experimental conditions, and avoids the error caused by the uneven concentration of the exogenous biological stimulation hormone liquid in the operation; the saline solution and polyethylene glycol penetrating fluid simulate the saline-alkali and drought stimulation under outdoor conditions, so that whether exogenous biostimulant has adverse resistance can be rapidly judged; finally, the natural growth condition of the rice root system can be directly observed through scale marks on the transparent box, the morphological indexes of the overground part and the underground part are compared and observed, the stress resistance of the biological stimulation hormone to salt and drought stress in the seedling stage of the rice is evaluated through the percentage of the yellow leaf number and the ratio of the length of the root system before and after exogenous biological stimulation, and the salt and drought resistance degree of different rice varieties can be screened out by the method. The method has the advantages of strong reliability of the identification result, simple operation, time and labor saving, improved investigation efficiency of later-stage root system data, convenient observation and popularization and application in agricultural scientific research institutions and seed enterprises.
Drawings
FIG. 1 is a schematic perspective view of a device for identifying exogenous biostimulant resistance to rice seedling stage in the invention.
FIG. 2 is a top view of the apparatus for identifying exogenous biostimulant for rice seedling stress resistance according to the present invention.
Fig. 3 is a schematic view showing the up and down movement of the black light-shielding laminate in the present invention.
Fig. 4 is a schematic view of the black light-shielding laminate of the present invention moving left and right.
In the figure: 1 transparent barrel, 2 box body, 3 black light-proof board, 4 absorbent cotton, 5 absorbent cotton, 6 open field planting cotton.
Detailed Description
The invention is further illustrated by means of the following examples, which are not intended to limit the scope of the invention. The experimental methods, in which specific conditions are not noted in the following examples, were selected according to conventional methods and conditions, or according to the commercial specifications. Unless otherwise stated, the temperature is usually at room temperature, and in the present invention, the room temperature is 10 to 30 ℃.
The experimental methods used in the following examples are conventional methods unless otherwise specified.
Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
As shown in figures 1-4, the device for identifying the stress resistance of exogenous biological stimulation hormone to rice seedling stage comprises a transparent barrel (62 chi 29 chi 20 cm) at the bottom, four equal-volume black light-resistant layers of box bodies I, II, III and IV and open field planting cotton; the bottom of the box body I-IV contains absorbent cotton, the rice seeds after being disinfected by hydrogen peroxide are uniformly sown on absorbent cotton after full water absorption, when the rice sprouts for 4-6 days reach 1-2cm, 3-4 rice seedlings with basically uniform sprouting are taken out and fixed on open field cotton (d=20mm), and the seed embryo is downwards placed. Fixing the open field cotton on a 12-hole black light-shielding layer, wherein 4 germinated seedlings are arranged in each hole of the light-shielding layer for standby; taking out the absorbent cotton at the bottom of the box, adding water to fix the volume to the position of the transparent barrel scale mark 9; after the operation is finished, the box bodies I-IV are clamped on the transparent barrel according to the marks, and the box bodies which are not used in the experiment can be taken out according to the experiment needs.
The transparent barrel is made of plastic hard, numbers 1-10 are carved on the outside of the transparent barrel, root length can be directly observed conveniently, root system indexes can be read out, and root system development conditions can be continuously shot and recorded in the growth process of seedlings.
The method for identifying the stress resistance of the exogenous biological stimulus to the seedling stage of the rice by using the equipment comprises the following steps:
1) The preparation of exogenous biological stimulation hormone for experiments stimulates the root systems of rice seedlings to fully absorb:
the exogenous biostimulant in the invention is polyglutamic acid.
After the rice seedlings grow to one leaf and one heart, adding 100-250mg/L of biological hormone into a treatment group box body, wherein the treatment concentration can be set to 3-5, after 5 days of treatment, obvious development difference trend of plant root systems and plant heights compared with ck can be observed, the plant growth can be promoted, at the moment, the plant is young, in order to prevent the seedlings from being burnt and green algae from breeding, no additional nutrient solution is needed in the period, and after the stimulation is finished, the seedlings and the black light-resistant laminate are taken down together and transferred into an empty box body;
2) Preparation of the identification saline solution for simulating salt stress treatment:
the method comprises the steps of stimulating rice seeds with a certain concentration of stimulin until roots fully absorb nutrition, injecting sodium chloride solution with a mass concentration of 2-5 permillage into a treatment group box body after 5d, and supplementing Hogland nutrient solution once every three days until 14d treatment is completed, wherein a control group is equal amount of clear water, and liquid in the box can evaporate along with the control group over time. The seedlings are placed in an artificial climate incubator with the temperature of 25 ℃ and the relative humidity of 80% +/-2% and the light intensity of 1600 lx;
3) The preparation of the polyethylene glycol permeate with high concentration and high molecular weight is identified and used for simulating drought stress treatment conditions:
the root of the seed is stimulated by a stimulus with a certain concentration to fully absorb nutrition, polyethylene glycol-6000 hypertonic solution with the mass concentration of 67, 100 and 200g/L is injected into the box body of the treatment group after 5 days, the control group is equal amount of clear water, the liquid in the box can be evaporated along with the control group along with the time, and Hogland nutrient solution is supplemented every three days until the treatment is completed during the stress period. The seedlings are placed in an artificial climate incubator with the temperature of 25 ℃, the relative humidity of 80% and the light intensity of 1600 lx;
4) Identification and evaluation of salt tolerance and drought tolerance of rice in seedling stage
a. The rice seedlings are taken out after being stressed in a climatic chamber at 25-30 ℃ for 14 days, the root length of the lower part of the box body is investigated and counted, and the transparent box body is marked with numbers 1-10, so that the index of the root system can be directly read out;
b. the root bud index, the root hair number, the salt tolerance coefficient and the drought tolerance coefficient are used as indexes for evaluating the excellent biological stimulation. A root system scanner (WinRHIZO, lin Yuan technology Co., ltd.) is used for counting and investigating the area of the upper yellow leaves and the quantity of root hairs; based on the measured values of the indices, an average value is calculated, and the individual items are calculated according to the following formula:
root bud index = rice seedling height/root length
Salt tolerance coefficient = relative Huang Shebi (number of yellow leaves per total number of leaves of treatment group) ×sodium chloride solution concentration×1000
Salt tolerance coefficient = relative Huang Shebi (number of yellow leaves per total number of leaves of treatment group) ×polyethylene glycol-6000 mass concentration/100
c. The evaluation was made according to root bud index on the following 3 scale:
(1) high stress-resistant biological hormone root bud coefficient >1.2
(2) 1.0< medium stress resistance organism stimulating hormone root bud coefficient <1.2
(3) Stress-free biological hormone root bud coefficient is less than 1.0
d. Evaluation was made according to the salt (drought) tolerance coefficient as registered below:
(1) high stress-resistant biological hormone salt tolerance (drought) coefficient <1
(2) The stress-free biological stimulation hormone salt tolerance (drought) coefficient is more than 1.
The invention completes the seedling treatment under the same device, ensures the consistent experimental conditions, and avoids the error caused by the uneven concentration of the exogenous biological stimulation hormone liquid in the operation; the saline solution and polyethylene glycol penetrating fluid simulate the saline-alkali and drought stimulation under outdoor conditions, so that whether exogenous biostimulant has adverse resistance can be rapidly judged; finally, the natural growth condition of the rice root system can be directly observed through scale marks on the transparent box, the morphological indexes of the overground part and the underground part are compared and observed, the stress resistance of the biological stimulation hormone to salt and drought stress in the seedling stage of the rice is evaluated through the percentage of the yellow leaf number and the ratio of the length of the root system before and after exogenous biological stimulation, and the salt and drought resistance degree of different rice varieties can be screened out by the method. The method has the advantages of strong reliability of the identification result, simple operation, time and labor saving, improved investigation efficiency of later-stage root system data, convenient observation and popularization and application in agricultural scientific research institutions and seed enterprises.
The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of the invention should be assessed as that of the appended claims.
Claims (7)
1. A device for identifying exogenous biological stimulation hormone on rice seedling stress resistance is characterized in that: the device comprises a transparent barrel, wherein a plurality of box bodies with the same volume are arranged in the transparent barrel, a water absorbing cotton layer is arranged at the bottom of the box body, a black light-shading plate with a plurality of through holes and a plurality of opening field planting cotton are arranged at the top of the box body, and scale marks are arranged on the side wall of the transparent barrel;
the method for identifying the stress resistance of the exogenous biological stimulus to the seedling stage of the rice adopts the device and specifically comprises the following steps:
1) Uniformly sowing the sterilized rice seeds after being sterilized by hydrogen peroxide on absorbent cotton which fully absorbs water, taking a plurality of rice seedlings with basically uniform germination, fixing the rice seedlings on open field cotton (d=20mm) when the rice sprouts to 1-2cm, and placing seed embryos downwards; fixing the open field cotton on a porous black light-shading layer, so that a plurality of germinated seedlings are arranged in each hole of the light-shading layer for standby; taking out the absorbent cotton at the bottom of the box, adding water to fix the volume to the marked positions of the graduation marks of the transparent barrel; after the operation is finished, the box body is clamped on the transparent barrel according to the mark;
2) Preparing exogenous biological hormone, adding the biological hormone into the box body after the seedlings grow to a leaf and a core in the step 1), treating for a period of time, taking down the seedlings and the black light-resistant laminate after the stimulation is finished, and transferring the seedlings and the black light-resistant laminate into an empty box body;
3) Preparing and identifying salt solution and high-concentration high-molecular-weight polyethylene glycol penetrating fluid, and respectively adding the salt solution and the high-concentration high-molecular-weight polyethylene glycol penetrating fluid into a box body where seedlings are positioned, wherein the salt solution and the high-concentration high-molecular-weight polyethylene glycol penetrating fluid are used for simulating salt stress treatment and drought stress treatment;
4) Identifying and evaluating salt tolerance and drought tolerance of rice in seedling stage;
the step 4) specifically comprises the following steps:
a. the rice seedlings are taken out after being stressed in a climatic chamber at 25-30 ℃ for 14 days, the root length of the lower part of the box body is investigated and counted, and the transparent box body is marked with numbers 1-10, so that the index of the root system can be directly read out;
b. taking root bud index, root hair number, salt tolerance coefficient and drought tolerance coefficient as indexes for evaluating the excellent biological stimulation; counting and investigating the area of the upper yellow leaf and the number of root hairs by using a root system scanner; based on the measured values of the indices, an average value is calculated, and the individual items are calculated according to the following formula:
root bud index = rice seedling height/root length
Salt tolerance coefficient = relative Huang Shebi sodium chloride solution concentration x 1000, relative Huang Shebi = treatment group yellow leaf number/total leaf number;
drought tolerance coefficient = relative Huang Shebi x polyethylene glycol-6000 mass concentration/100, relative Huang Shebi = treatment group yellow leaf number/total leaf number;
c. the evaluation was made according to root bud index on the following 3 scale:
(1) high stress-resistant biological hormone root bud coefficient >1.2
(2) 1.0< medium stress resistance organism stimulating hormone root bud coefficient <1.2
(3) Stress-free biological hormone root bud coefficient is less than 1.0
d. Evaluation was made according to the following registration according to the salt tolerance or drought tolerance coefficient:
(1) high stress-resistant biological hormone salt tolerance or drought tolerance coefficient <1
(2) The stress-free biological stimulation hormone salt tolerance or drought tolerance coefficient is more than 1.
2. The device for identifying stress resistance of exogenous biological hormones to rice seedlings according to claim 1, wherein: the transparent barrel is a hard plastic barrel.
3. The device for identifying stress resistance of exogenous biological hormones to rice seedlings according to claim 1, wherein: the concentration of the exogenous biological hormone in the step 2) is 100-250mg/L, the treatment concentration is set to 3-5, and the obvious development difference trend of plant root systems and plant heights compared with ck is observed obviously after 5 days of treatment.
4. The device for identifying stress resistance of exogenous biological hormones to rice seedlings according to claim 1, wherein: the step 3) of simulated salt stress treatment specifically comprises the following steps: preparing sodium chloride solution with mass concentration of 2-5%and respectively injecting into a box body where rice seeds treated by exogenous biological stimulation are located for 5 days, wherein a control group is equal amount of clear water, liquid in the box is gradually evaporated, hogland nutrient solution is supplemented every three days during stress until 14 days are finished, and seedlings are placed into a phytotron for culture.
5. The device for identifying stress resistance of exogenous biological hormones to rice seedlings according to claim 1, wherein: the simulated drought stress treatment in the step 3) specifically comprises the following steps: preparing 67, 100 and 200g/L high-concentration high-molecular-weight polyethylene glycol permeate, respectively injecting the high-concentration high-molecular-weight polyethylene glycol permeate into a box body where rice seeds treated by exogenous biological stimulation are located for 5 days, gradually evaporating the liquid in the box body by using equal amount of clear water as a control group, supplementing Hogland nutrient solution once every three days during stress until the treatment is completed, and culturing seedlings in an artificial climate incubator.
6. The device for identifying stress resistance of exogenous biological stimulus to rice seedlings according to claim 5, wherein: the polyethylene glycol permeate in the step 3) is polyethylene glycol-6000 hypertonic solution.
7. The device for identifying stress resistance of exogenous biological hormones to rice seedlings according to any of claims 4-5, wherein the device is characterized by: the temperature in the artificial climate incubator is 25 ℃, the relative humidity is 80% +/-2%, and the light intensity is 1600lx.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210665116.1A CN115005034B (en) | 2022-06-14 | 2022-06-14 | Device and method for identifying stress resistance of exogenous biological stimulus to rice seedling stage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210665116.1A CN115005034B (en) | 2022-06-14 | 2022-06-14 | Device and method for identifying stress resistance of exogenous biological stimulus to rice seedling stage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115005034A CN115005034A (en) | 2022-09-06 |
| CN115005034B true CN115005034B (en) | 2023-12-08 |
Family
ID=83075170
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210665116.1A Active CN115005034B (en) | 2022-06-14 | 2022-06-14 | Device and method for identifying stress resistance of exogenous biological stimulus to rice seedling stage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115005034B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116267466B (en) * | 2023-01-16 | 2024-04-16 | 湖南农业大学 | Method for cultivating strong seedlings and improving cold resistance of seedlings by light control |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203801370U (en) * | 2014-05-09 | 2014-09-03 | 中国科学院烟台海岸带研究所 | Test device for simulating plant water logging stress |
| CN104830899A (en) * | 2015-05-21 | 2015-08-12 | 兰州理工大学 | Cultivation method of strong salt-tolerant and drought-resistant sugarbeet |
| CN109819714A (en) * | 2019-04-03 | 2019-05-31 | 扬州大学 | A kind of method that can largely screen rice seedling Salinity tolerance germplasm |
| CN209749267U (en) * | 2019-04-16 | 2019-12-10 | 山东农业工程学院 | Detachable multifunctional plant root observation barrel |
| CN111492917A (en) * | 2020-04-14 | 2020-08-07 | 山东省水稻研究所 | Method for efficiently identifying salt tolerance of rice seedlings |
| CN111492919A (en) * | 2020-04-16 | 2020-08-07 | 江苏沿海地区农业科学研究所 | High-salt-tolerance rice germplasm identification and screening method |
| CN112262637A (en) * | 2020-10-30 | 2021-01-26 | 广东海洋大学 | Salt tolerance identification and evaluation method for hybrid rice variety |
| CN114451282A (en) * | 2022-01-14 | 2022-05-10 | 乌兰察布市农林科学研究所 | Indoor rapid identification and screening method for salt and alkali-resistant oats |
| CN114568276A (en) * | 2022-02-08 | 2022-06-03 | 广东省农业科学院水稻研究所 | Salt-tolerant identification method for rice and device used by method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9434957B2 (en) * | 2012-06-28 | 2016-09-06 | The Penn State Research Foundation | Methods of improving drought tolerance and seed production in rice |
-
2022
- 2022-06-14 CN CN202210665116.1A patent/CN115005034B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203801370U (en) * | 2014-05-09 | 2014-09-03 | 中国科学院烟台海岸带研究所 | Test device for simulating plant water logging stress |
| CN104830899A (en) * | 2015-05-21 | 2015-08-12 | 兰州理工大学 | Cultivation method of strong salt-tolerant and drought-resistant sugarbeet |
| CN109819714A (en) * | 2019-04-03 | 2019-05-31 | 扬州大学 | A kind of method that can largely screen rice seedling Salinity tolerance germplasm |
| CN209749267U (en) * | 2019-04-16 | 2019-12-10 | 山东农业工程学院 | Detachable multifunctional plant root observation barrel |
| CN111492917A (en) * | 2020-04-14 | 2020-08-07 | 山东省水稻研究所 | Method for efficiently identifying salt tolerance of rice seedlings |
| CN111492919A (en) * | 2020-04-16 | 2020-08-07 | 江苏沿海地区农业科学研究所 | High-salt-tolerance rice germplasm identification and screening method |
| CN112262637A (en) * | 2020-10-30 | 2021-01-26 | 广东海洋大学 | Salt tolerance identification and evaluation method for hybrid rice variety |
| CN114451282A (en) * | 2022-01-14 | 2022-05-10 | 乌兰察布市农林科学研究所 | Indoor rapid identification and screening method for salt and alkali-resistant oats |
| CN114568276A (en) * | 2022-02-08 | 2022-06-03 | 广东省农业科学院水稻研究所 | Salt-tolerant identification method for rice and device used by method |
Non-Patent Citations (2)
| Title |
|---|
| 宛氏拟青霉提取物对盐胁迫下水稻幼苗的生理适应性;王晓琪;姚媛媛;刘之广;陈宝成;张民;马金昭;王庆彬;;农业资源与环境学报(01);98-105 * |
| 植物对盐胁迫的响应及耐盐调控的研究进展;王康君;樊继伟;陈凤;李强;孙中伟;郭明明;张广旭;郑国良;;江西农业学报(12);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115005034A (en) | 2022-09-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Demir et al. | Effect of salt priming on germination and seedling growth at low temperatures in watermelon seeds during development | |
| CN102123581B (en) | Method for growing plants | |
| CN103931475B (en) | Salt sward seedling cultural method | |
| CN104429971B (en) | Tissue culture seedling-raising method of millettia fordii dunn | |
| CN108207167B (en) | Method for improving germination rate of tomato seeds under drought stress and observation device | |
| CN115005034B (en) | Device and method for identifying stress resistance of exogenous biological stimulus to rice seedling stage | |
| CN108521932B (en) | Method for promoting germination of Chinese angelica propagation substrate | |
| CN100407897C (en) | Roxburgh anoectochilus cultivating device and technology | |
| CN102172147B (en) | Method for improving winter resistance of turf by adopting garbage compost filtrate | |
| CN104651474B (en) | A kind of rapid identification method of muskmelon powdery mildew biological strain | |
| CN114128611A (en) | Indoor annual sowing and seedling raising method for alpine rhododendrons | |
| CN109511534B (en) | Berry seed sowing and breeding method | |
| CN110839522A (en) | Method for controlling growth and development of rice | |
| CN114711132B (en) | Water culture method for rapidly verifying fertilizer efficiency and application thereof | |
| CN110199854A (en) | A kind of identification method of Seedling Stage Salt Tolerance of Barley | |
| CN113331013B (en) | Method for sowing and forcing culture of lilium pinosum | |
| CN107018707A (en) | A kind of method for identifying rape seed Germination And Seedling low temperature tolerance characteristicses | |
| CN112616591A (en) | Screening method of corn varieties with low enrichment of cadmium | |
| CN109496704B (en) | Method for quickly growing and cultivating arabidopsis thaliana | |
| CN105265477B (en) | Application of the ethephon (CEPHA),2-(chloroethyl) phosphonic acid in English grass germinating energy and drought tolerance is improved | |
| CN113336595B (en) | Gene editing tobacco black nutrient solution formula, water culture device and water culture method | |
| CN110810173A (en) | Method for identifying cold resistance of cabbage type winter rape by root system characters | |
| CN108668748A (en) | A kind of implantation methods of peach | |
| CN213095102U (en) | Indoor experimental plant seed germination or seedling raising device | |
| CN213719230U (en) | Device for screening soybean seedling-stage root drought-resistant waterlogging-resistant varieties |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |