CN213719230U

CN213719230U - Device for screening soybean seedling-stage root drought-resistant waterlogging-resistant varieties

Info

Publication number: CN213719230U
Application number: CN202022254597.6U
Authority: CN
Inventors: 宋丽; 潘贞志; 戴毅; 陈林
Original assignee: Yangzhou University
Current assignee: Yangzhou University
Priority date: 2020-10-12
Filing date: 2020-10-12
Publication date: 2021-07-20
Anticipated expiration: 2030-10-12

Abstract

The invention relates to the technical field of seedling screening test equipment, in particular to a device for screening drought-resistant and waterlogging-resistant varieties of soybean seedling root systems, which comprises a support and a plant culture device, wherein a plurality of insertion holes are formed in the bottom of the support, the plant culture device is inserted into the insertion holes, and a bottom cover is arranged at the bottom of the plant culture device; the bottom of the plant culture device is provided with a first large-diameter section, the bottom of the first large-diameter section is provided with a floor drain, a second large-diameter section is arranged above the plant culture device, the bottom of the second large-diameter section is provided with a seeding pore plate, and a plurality of seedling holes are formed in the seeding pore plate; and plant culture soil or nutrient solution is filled between the floor drain and the seeding pore plate. The device can realize the accurate control of the watering amount, has high accuracy of test results, can quickly and comprehensively evaluate the drought and waterlogging stress tolerance and the recovery capability of soybean varieties, and is suitable for screening a large number of varieties with strong drought and waterlogging tolerance because of easy operation and small volume.

Description

Device for screening soybean seedling-stage root drought-resistant waterlogging-resistant varieties

Technical Field

The invention belongs to the technical field of seedling screening test equipment, and relates to a device for screening soybean seedling-stage root drought-resistant waterlogging-resistant varieties.

Background

Drought and flood are one of the common natural disasters in crop production, and severely limit the production and distribution of many important crops in the world. When the soil is lack of water to a certain degree, the root system of the plant firstly senses a signal of water shortage stress, so that the development of the root is inhibited, the water content in plant cells is reduced, the cell volume is reduced, the turgor pressure of the cells is reduced, and finally the growth of the plant is inhibited and even dies. And when the soil moisture is too much, flood disasters are easily caused, and the flood disasters are mainly divided into two types: damp damage and waterlogging damage. The wet damage refers to excessive moisture in soil, mainly refers to that gaps among soil particles are filled with moisture, so that oxygen is deficient, and is caused by continuous rainy days and cloudy days. Waterlogging is caused when a part or all of the plants are submerged by ponding, and is often caused after flooding or rainstorm in low-lying and swamp areas. The moisture damage and waterlogging damage can cause oxygen deficiency in soil, the respiration of roots is weakened, the absorption of roots to nutrient substances is hindered, the aerobic respiration is replaced by the anaerobic respiration, final products of the anaerobic respiration, such as alcohol and lactic acid, and toxic substances generated by anaerobic bacteria, such as hydrogen sulfide, ammonia and the like, can generate toxic action on the roots of crops, the growth cannot be normally carried out, and the crops are dead.

The soybean is a main grain and oil dual-purpose crop in China and also an important source of high-quality protein and edible oil for human beings, but the soybean has a less developed root system and a large water demand in the growing period. When drought or flood stress is received, the plant root system firstly reacts and quickly sends a signal, so that the whole plant reacts to the drought or flood stress, meanwhile, the morphological structure and the material accumulation of the root system also correspondingly change, the growth and development of the overground part are influenced, the plant is helped to reduce the damage caused by the environmental stress, the differences of the drought and flood resistance of different soybean varieties are obvious, and how to quickly and massively identify and screen the variety with strong drought and flood resistance has important significance for production and breeding. At present, a device suitable for evaluating drought resistance and waterlogging tolerance of a large number of soybean varieties and stress relief of plant restoration capacity is not found, the device for drought treatment is mainly a flowerpot at present, the method is large in occupied area and labor-consuming to carry, and due to the fact that the height of the flowerpot is not high, the method is not beneficial to longitudinal growth of roots and is not beneficial to evaluating the drought resistance and waterlogging tolerance of the varieties from root phenotype. The drought and waterlogging resistance of soybean varieties needs to be comprehensively evaluated, the root system form after drought and waterlogging stress treatment needs to be observed, and the recovery capability of plants needs to be evaluated after stress is relieved, so that the drought and waterlogging resistance of soybean seedlings cannot be comprehensively and accurately evaluated by the conventional device at present.

Disclosure of Invention

In order to solve the problems, the application provides a device for screening drought-resistant and waterlogging-tolerant varieties of soybean seedling roots, which can carry out drought-waterlogging treatment and evaluation of plant recovery capability after drought-waterlogging stress is relieved to different degrees. The experimental device realizes the functions of normal culture, drought and waterlogging treatment and stress relief of soybean seedlings by accurately controlling the watering amount, can completely take out the whole root system for subsequent observation of the root system shape, has high accuracy of test results, can quickly and comprehensively evaluate drought and waterlogging stress tolerance and recovery capability of soybean varieties, and is suitable for screening a large number of varieties with strong drought resistance and waterlogging resistance because of easy operation and small volume.

In order to realize the purpose of the invention, the technical scheme adopted by the invention is as follows:

a device for screening drought-resistant and waterlogging-resistant varieties of soybean seedling-stage root systems comprises a support and a plant culture device, wherein a plurality of insertion holes are formed in the bottom of the support, the plant culture device is inserted into the insertion holes, and a bottom cover is arranged at the bottom of the plant culture device; the bottom of the plant culture device is provided with a first large-diameter section, the bottom of the first large-diameter section is provided with a floor drain, a second large-diameter section is arranged above the plant culture device, the bottom of the second large-diameter section is provided with a seeding pore plate, the seeding pore plate is provided with a plurality of seedling holes, and the seedling holes are provided with a sponge prick for fixing seedlings; and plant culture soil or nutrient solution is filled between the floor drain and the seeding pore plate.

Furthermore, the device for screening the drought-resistant and waterlogging-resistant soybean variety for the root system in the seedling stage further comprises a water tank, and the water tank is placed at the bottom of the plant culture device.

Furthermore, the water tank is made of plastic.

Further, the jack is circular with the diameter of 80 mm.

Furthermore, a plurality of floor drain holes are formed in the floor drain, and the number of the floor drain holes is 10-50; the floor drain is made of stainless steel.

Furthermore, the size of the floor drain is 70mm in outer diameter, 47mm in inner diameter and 8mm in height.

Further, the number of the floor drain holes is 37.

Furthermore, the plant culture device is tubular and is made of PVC.

Further, a handle is arranged above the device.

Further, the plant culture soil is diatomite.

Further, the support is 1m long and 0.5m wide.

Further, pulleys are arranged at four corners of the bottom of the support and are universal wheels.

Further, be provided with a plurality of seedling holes on the seeding orifice plate, the quantity in seedling hole is 6.

Furthermore, the sponge is rolled into a sponge strip with the length of 5cm, the width of 1cm and the height of 0.2 cm.

Compared with the prior art, the application has the following beneficial effects:

this device uses the PVC pipe, compares with traditional cultivated in a pot, and is whole light, easily removes, and is small, saves space, repeatedly usable. Simple operation and suitability for large-batch variety screening. Can water different volumes as required, the degree of accurate control drought and waterlogging, and traditional cultivated in a pot can only realize the drought of a degree at the same time, this device reduces the experimental error greatly in comparison, and can adjust the degree of coerceing, the experimental period has been shortened greatly, after the waterlogging is handled, the accessible takes off bottom cover, twist the lid after draining the water and normally water and still can evaluate the resilience after the removal of the waterlogging stress of different soybean varieties, relative before can only evaluate the anti-stress ability under the waterlogging stress of drought, use the device can more comprehensive aassessment the tolerance to coerceing of different soybean varieties. Meanwhile, diatomite is used for filling, and the diatomite is easy to pour out after treatment, so that a complete root system can be obtained, the root system form after stress can be conveniently observed subsequently, and the physiological indexes of the total root length, the total root surface area, the root volume, the root diameter, the root system elongation capacity, the root system growth rate and the like which can reflect the drought and waterlogging resistance capacity can be measured.

Drawings

FIG. 1 is a schematic view of a stent;

FIG. 2 is a schematic view of a plant cultivation apparatus;

figure 3 is a schematic view of a floor drain;

FIG. 4 is a schematic view of a seeding aperture plate;

FIG. 5 is a schematic view of a sink.

1. A support; 2. a pulley; 3. a handle; 4. a jack; 5. a plant cultivation device; 6. a bottom cover; 7. a first major diameter section; 8. a floor drain; 9. a second major diameter section; 10. a seeding pore plate; 11. seedling holes are formed; 12. a water tank.

Detailed Description

Example 1

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention.

As shown in the figure, the utility model provides a device for screening soybean seedling stage root system drought-resistant waterlogging-resistant varieties, which comprises a bracket 1 and a plant culture device 5, wherein the bracket 1 is 1m long and 0.5m wide; the upper part of the bracket is in a grid shape, corresponds to the bottom jacks and is used for fixing the plant culture device so that the plant culture device cannot topple over; the support bottom four corners department is provided with pulley 2, the pulley is the universal wheel, conveniently removes. The handles 3 are arranged above the device, and the number of the handles is 1 or 2, so that the support can be conveniently moved; the bottom of the bracket is provided with a plurality of jacks 4, and the jacks are circular with the diameter of 80 mm;

a plant culture device 5 is inserted into the insertion hole 4, and is tubular and made of PVC; the diameter is 75mm, and the length is 40 cm; the bottom of the plant culture device 5 is provided with an inspection opening, the inspection opening is provided with a bottom cover 6, and when the bottom cover is fastened, a good water retention function can be realized. The bottom of the plant cultivation device is provided with a first large-diameter section 7, and the outer diameter of the first large-diameter section 7 is larger than 75 mm; preferably, the outer diameter of the first large-diameter section 7 is 80 mm; the first large-diameter section and the pipe wall of the plant culture device form an annular step; placing a plant culture device in a jack of a support through an annular step of a first large-diameter section, wherein a floor drain 8 is arranged at the bottom of the first large-diameter section, and the floor drain is made of stainless steel; the size of the floor drain is 70mm in outer diameter, 47mm in inner diameter and 8mm in height. The floor drain is provided with a plurality of floor drain holes, and the number of the floor drain holes is 10-50, preferably 37.

A second large-diameter section 9 is arranged above the plant culture device, and the outer diameter of the second large-diameter section 9 is larger than 75 mm; the second large-diameter section 9 and the pipe wall of the plant culture device form an annular step; a seeding pore plate 10 is arranged at the bottom of the second large-diameter section 9, a plurality of seedling pores 11 are arranged on the seeding pore plate 10, and each seedling pore is provided with a sponge prick for fixing seedlings; the sponge is rolled into a sponge strip with the length of 5cm, the width of 1cm and the height of 0.2 cm. The number of the seedling holes is 4-10, preferably 6. Plant culture soil or nutrient solution is filled between the floor drain 8 and the seeding pore plate 10, and the height of the plant culture soil is less than 3cm of the pipe orifice of the plant culture device. The plant culture soil is diatomite with the grain size of 3-5mm, has good air permeability and is convenient for taking out a complete root system for observation.

The device for screening the drought-resistant and waterlogging-resistant soybean variety for the root system in the seedling stage further comprises a water tank 12, wherein the water tank is 0.95m long, 0.45m wide and 5cm high, is placed at the bottom of the plant culture device and is used for receiving water. The water tank is made of plastic.

When the device is used for carrying out normal culture on soybeans, diatomite can be used for culture or water culture, when the diatomite is used for culture, the device is utilized to fill the diatomite into the tubes until the positions of the tube openings are 3cm, 3-5 seeds can be sown in each tube, and the device is watered until the diatomite just submerges; when water culture is carried out, bean seedlings are cultured in a seedling culture pot until cotyledons are slightly stretched, the seedlings are wrapped by a sponge binding tape and are placed into a seeding hole plate for fixation, culture solution is poured into a PVC pipe until the pipe opening, and the PVC pipe is placed in a greenhouse for culture, wherein the temperature is 28 ℃, the illumination intensity is 10000 lux, the illumination time is 16 hours, the illumination time is 8 hours, the darkness is 8 hours, and the humidity is 40%. When drought treatment is carried out, taking out the seeding pore plate, putting in diatomite, normally watering until the third or fourth leaf stage, removing the bottom cover, suspending the device, completely flowing out water, covering the bottom cover, then watering different amounts of water to realize drought effects of different degrees, removing the bottom cover to realize maximum drought, and then screwing the bottom cover up to carry out rehydration treatment; when carrying out the flood processing, cultivate bean seedling to cotyledon micro-expanse in the basin of growing seedlings, move bean seedling to the device afterwards, water planting to bean seedling growth to suitable period, with intraductal water of irritating to the mouth of pipe in order to realize the flood effect, remove the messenger water outflow with the bottom lid after the processing, later twist the bottom lid and normally water and can make the flood coerce and relieve. In conclusion, the device realizes the functions of normal soybean culture, drought and waterlogging treatment and rehydration. And after the treatment is finished, pouring out the diatomite or the culture solution from the upper part to obtain a complete root system for subsequent morphological observation and measurement of the root system.

Taking off the bottom cover, stopping watering for about 5 days, making the plant begin to wilt, and making the plant have more wilting after about 7 days, then screwing on the bottom cover, watering for about 50ml, and after 24 hours, making the plant recover normal growth. The device is proved to be suitable for the growth of soybean in seedling stage, has good drought stress treatment and rehydration functions, and can be better used for screening soybean drought-resistant varieties and researching drought stress molecular mechanisms.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A device for screening drought-resistant and waterlogging-resistant varieties of soybean seedling-stage root systems is characterized by comprising a support and a plant culture device, wherein a plurality of insertion holes are formed in the bottom of the support, the plant culture device is inserted into the insertion holes, and a bottom cover is arranged at the bottom of the plant culture device; the bottom of the plant culture device is provided with a first large-diameter section, the bottom of the first large-diameter section is provided with a floor drain, a second large-diameter section is arranged above the plant culture device, the bottom of the second large-diameter section is provided with a seeding pore plate, the seeding pore plate is provided with a plurality of seedling holes, and each seedling hole is provided with a sponge prick for fixing seedlings; and plant culture soil or nutrient solution is filled between the floor drain and the seeding pore plate.

2. The device for screening the drought-resistant and waterlogging-resistant varieties of the soybean seedling-stage root systems, according to claim 1, is characterized by further comprising a water tank, wherein the water tank is placed at the bottom of the plant cultivation device.

3. The device for screening soybean seedling-stage root system drought-resistant waterlogging-resistant varieties as claimed in claim 2, wherein the water tank is made of plastic.

4. The device for screening soybean seedling-stage root drought-resistant waterlogging-resistant varieties is characterized in that the insertion holes are circular and 80mm in diameter.

5. The device for screening the drought-resistant waterlogging-resistant soybean seedling-stage root system varieties is characterized in that a plurality of floor drains are arranged on the floor drain, the number of the floor drains is 10-50, and the floor drain is made of stainless steel.

6. The device for screening the drought-resistant waterlogging-resistant soybean variety for the root system in the seedling stage as claimed in claim 1, wherein the plant cultivation device is tubular and is made of PVC.

7. The device for screening soybean seedling-stage root system drought-resistant waterlogging-resistant varieties is characterized in that a handle is arranged above the device.

8. The device for screening soybean seedling-stage root drought-resistant waterlogging-resistant varieties is characterized in that the plant culture soil is diatomite.

9. The device for screening soybean seedling-stage root drought-resistant waterlogging-resistant varieties is characterized in that the length of the bracket is 1m, and the width of the bracket is 0.5 m.

10. The device for screening the drought-resistant and waterlogging-resistant soybean variety for the root system in the seedling stage is characterized in that pulleys are arranged at four corners of the bottom of the support and are universal wheels.