CN104833776A - Cotton flooding tolerance evaluation method - Google Patents
Cotton flooding tolerance evaluation method Download PDFInfo
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- CN104833776A CN104833776A CN201510233613.4A CN201510233613A CN104833776A CN 104833776 A CN104833776 A CN 104833776A CN 201510233613 A CN201510233613 A CN 201510233613A CN 104833776 A CN104833776 A CN 104833776A
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Abstract
The invention discloses a cotton flooding tolerance evaluation method comprising the steps of irrigating after seeding selected cotton seeds, and completely immersing the cotton seeds into water to carry out water flooding treatment until water is about 1cm higher than a sand surface; and maintaining water flooding treatment for 6 days, surveying the germination rate of the cotton seeds after seeding for 15 days, and evaluating according to the relative germination rate. A rapid, simple and convenient cotton flooding tolerance evaluation method is created on the basis that the germination rate of the cotton seeds is changed after water flooding, and the flooding tolerance appraisals on 83 parts of materials prove that the method can be used for obtaining a relatively reliable result, objectively reflecting the real situation of cotton flooding, rapidly determining the flooding tolerance of the cotton from the view of the relative germination rate of cotton and sieving varieties/resources with strong flooding tolerance.
Description
Technical field
The present invention relates to cotton culture and breeding field, is exactly one to grow cotton resistance to overhead flooding injury evaluation method.
Background technology
Cotton is one of most important industrial crops of China, cultivated area about 5,000,000 hectares, is distributed in the Huanghe valley, the Yangtze river basin and Northwest inland area; Its growth cycle is long, is the warm dry crop of a kind of happiness.
Each period of growing of cotton all needs enough moisture, but soil moisture is too much, soil air can be caused rare, easy generation noxious material, thus affect growth rate of cotton plant growth and normal physiological Mechanism function, show as rotten, dead seedling, emergence rate be low, leaf chlorosis turns yellow and comes off; Finally cause production declining, quality reduces.Under unusual weather conditions condition, the scope that cotton disaster occurs is wide, frequency is high, harm is heavy, cause regional or national output heavy losses or quality decline to a great extent (the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute. " Cotton cultivation ". Shanghai: science tech publishing house, 2013).The ground such as Anhui, Jiangsu, Hubei about have the cotton in 3/4ths times because of waterlogged disaster Severe Reduction, damage or crop failure caused by waterlogging becomes one of main limiting factor of these regional cotton yield stable yields.Cause the present situation of rainfall amount increase and spatial and temporal distributions exception thereof in the face of climate change, the impact of reduction waterlogged disaster on cotton becomes the important subject that agricultural production at present and in the future faces.
The approach of current minimizing damage or crop failure caused by waterlogging is mainly and carrys out lowering of watertable by the construction of water conservancy projects, reaches water drainage and falls stain; The production practices of each cotton region prove, improve cotton field irrigation and drainage system with functions, strengthen cotton field drainage works, and to alleviating cotton field waterlogged disaster, improving output of cotton has significant effect.
Seed selection resistance to overhead flooding injury kind solves the most basic effective method of waterlogged disaster.The waterlogging capacity variance of different genotype cotton variety is comparatively large, by setting up suitable waterlogging evaluation method, and can more effective select target genotypic variety.The qualification of cotton resistance to overhead flooding injury is then the basis of carrying out the waterlogging breeding of cotton.Carry out quantity research greatly in the form that many scholars occur cotton after different times suffers water logging stress, physiological Mechanism change etc., but report has been rarely had to the resistance to overhead flooding injury research of each kind.Adopt mode of appearance index, set up one simple, objectively respond cotton damage or crop failure caused by waterlogging truth, and the method for rapid, high volume resistance to overhead flooding injury evaluation can be carried out to Resources of Cotton, waterlogging Screening germplasm efficiency can be improved, accelerate the waterlogging breeding process of cotton, for the waterlogging breeding of cotton lays the foundation.
Summary of the invention
The object of this invention is to provide one to grow cotton waterlogging authenticate technology, be intended to set up a kind of result reliable and stable, simple to operate, be suitable for the resistance to overhead flooding injury brief appraisal method of rapid screening cotton waterlogging-tolerant variety in enormous quantities.
The present invention adopts following technical scheme
One grows cotton resistance to overhead flooding injury evaluation method, it is characterized in that, comprises the following steps:
(1) select stalwartness, the cotton seed that full, consistance is good as test material;
(2) by selected cotton seed, several flat little plastic cups, gauze about 10% disinfectant with hydrogen peroxide 15-20min, distilled water rinsing about 4 times, loads the fine sand of equivalent in flat little plastic cup;
(3) set up phytotron: its temperature is about 28 DEG C, intensity of illumination is about 1250lx, illumination every day about 8h;
(4) what selected cotton seed is seeded in step (2) is equipped with in the flat little plastic cup of fine sand, is after planting placed in flat large plastic tub and pours water, cover Shamian Island about 1cm be as the criterion with water; Continuously submerging 6d, with the cotton seed of planting under normal water management for contrast; After planting 15d investigates germination rate of cotton seed, carries out resistance to overhead flooding injury evaluation according to relative germination rate.
Described one grows cotton resistance to overhead flooding injury evaluation method, it is characterized in that:
In process of the test, submerging treatment needs change water every day, to prevent rotten seed on nomospermous impact; Keep the skin wet in time to correlating.
Described one grows cotton resistance to overhead flooding injury evaluation method, it is characterized in that:
Described flat little plastic cup is common flat water glass, opens several aperture, repave gauze bottom it, and keeping leaks does not leak sand, is convenient to change water simultaneously.
Described one grows cotton resistance to overhead flooding injury evaluation method, it is characterized in that:
Described submerging treatment method is " two basin (cup) method ", spread gauze by bottom opening, flat little plastic cup after planting neatly puts in flat large plastic tub, pour water in the flat large plastic tub of submerging treatment again, cover Shamian Island about 1cm to water.
Described one grows cotton resistance to overhead flooding injury evaluation method, it is characterized in that:
Described cotton seeds to be measured is unified is light seed, can avoid the experimental error caused because of the difference between hair seed and light seed.
Described one grows cotton resistance to overhead flooding injury evaluation method, it is characterized in that, the concrete evaluation method in step (4) for: the submerging treatment germination percentage a surveyed is waterflooding 6d, the after planting germination percentage of 15d cotton seed; The contrast germination percentage b surveyed is normal water management, the after planting germination percentage of 15d cotton seed;
Described waterlogging index x is relative germination rate, and namely waterlogging index x=submerging treatment germination percentage a/ contrasts germination percentage b × 100%;
If waterlogging index x >=50, cotton variety to be measured is highly waterlogging type cotton variety;
If waterlogging index 30≤x < 50, cotton variety to be measured is waterlogging type cotton variety;
If waterlogging index 10≤x < 30, cotton variety to be measured is intolerant to waterlogging type cotton variety;
If waterlogging index x < 10, cotton variety to be measured is pole intolerant to waterlogging type cotton variety.
Beneficial effect of the present invention is as follows:
(1) after the present invention is based on waterflooding, germination rate of cotton seed changes, set up a resistance to overhead flooding injury evaluation method growing cotton quick, easy, and by identifying the resistance to overhead flooding injury of 83 parts of materials, prove that this technical method can obtain result comparatively reliably, substantially cotton damage or crop failure caused by waterlogging truth can be reflected objectively, its resistance to overhead flooding injury can be determined fast from cotton relative germination rate, filter out kind/resource that resistance to overhead flooding injury is strong.
(2) the present invention's waterflooding and the germination percentage contrasted, calculating relative germination rate can carry out resistance to overhead flooding injury qualification to each kind of cotton, sets up evaluation method; It is simple to operation, only need observe waterflooding can draw resistance to overhead flooding injury evaluation result fast with the germination contrasted, and through repeatedly repeated authentication result is consistent, illustrates that the method result is reliable and stable, and simple to operate, be suitable for rapid screening cotton resistance to overhead flooding injury kind in enormous quantities; The method material therefor cost is low, simultaneously saving of work and time.
Embodiment
example 1
1, Shamian Island 1cm is crossed in the waterflooding of indoor waterflooding identification method, continuously submerging 6d.The 83 parts of kind/resources selecting the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute's germplasm resource bank and each cotton breeding unit to provide are test material.Each materials control and waterflooding germination percentage after investigation sowing 15d are that index is evaluated with relative germination rate, and the deciding grade and level scope of agriculture products.
2, each kind of selected cotton seed selects the healthy and strong full seed of seed as design test material; Reject damaged, injured seed, avoid because seed individual difference affects qualification result; The cotton seed chosen is placed in 10%H
2o
2middle sterilization, rinsed clean.
3, Sha Peifa cultivation sand used is undertaken by described technical scheme, and flat little plastics cup sand amount is the 1/2--2/3 of its volume.
4, sow every glass of evenly sowing 10 cotton seeds, each materials control and waterflooding are all established and are repeated for 10 times.Waterflooding method is " two basin (cup) method ", spreads gauze, neat the putting in flat large plastic tub of flat little plastic cup after planting, then pours water in the flat large plastic tub of submerging treatment, cover Shamian Island 1cm to water by bottom opening.
5, environment conditioning is set to 28 DEG C by cotton germination standard GB/T3543.4 temperature, and humidity is 80%, and intensity of illumination is 1250lx, illumination every day 8h.After planting, control treatment waters sufficient moisture to each material, and keeps the skin wet in time; Submerging treatment starts to pour water, 1cm to Shamian Island, and changes water every day, prevents rotten cotton seed on the impact of other seed.After waterflooding 6d, drain moisture, afterwards by normal germination test requirement management.
6, the waterlogging grade of cotton really normal root according to the germination percentage phenotype of cotton seeds after waterflooding, select it to compare with the corresponding germination percentage contrasted, be that waterlogging identification of indicator carries out the indoor resistance to overhead flooding injury evaluation of cotton variety with relative germination rate.Assessment grade is in table 1.
Table 1 cotton resistance to overhead flooding injury evaluation of programme and rank name
7,
cotton variety, resource resistance to overhead flooding injury qualification result
Table 2 83 parts of waterlogging indexes of cotton variety/resource and waterlogging grade
Example 2
According to the result of example 1, choose the different kind/resource of resistance to overhead flooding injury totally 6 parts multiple batches of checking is carried out to this authentication method.Result according to table 3 can be found out, each kind, resource are consistent with example 1 opinion rating, illustrates that this resistance to overhead flooding injury authentication method is reliable and stable.
The waterlogging index of table 3 cotton variety/resource and waterlogging grade
Claims (6)
1. one to grow cotton resistance to overhead flooding injury evaluation method, it is characterized in that, comprise the following steps:
(1) select stalwartness, the cotton seed that full, consistance is good as test material;
(2) by selected cotton seed, several flat little plastic cups, gauze about 10% disinfectant with hydrogen peroxide 15-20 min, distilled water rinsing about 4 times, loads the fine sand of equivalent in flat little plastic cup;
(3) set up phytotron: its temperature is about 28 DEG C, intensity of illumination is about 1250 lx, illumination every day 8 about h;
(4) what selected cotton seed is seeded in step (2) is equipped with in the flat little plastic cup of fine sand, is after planting placed in flat large plastic tub and pours water, cover Shamian Island about 1cm be as the criterion with water; Continuously submerging 6 d, with the cotton seed of planting under normal water management for contrast; After planting 15 d investigate germination rate of cotton seed, carry out resistance to overhead flooding injury evaluation according to relative germination rate.
2. according to claim 1 one to grow cotton resistance to overhead flooding injury evaluation method, it is characterized in that:
In process of the test, submerging treatment needs change water every day, to prevent rotten seed on nomospermous impact; Keep the skin wet in time to correlating.
3. according to claim 1 one to grow cotton resistance to overhead flooding injury evaluation method, it is characterized in that:
Described flat little plastic cup is common flat water glass, opens several aperture, repave gauze bottom it, and keeping leaks does not leak sand, is convenient to change water simultaneously.
4. according to claim 1 one to grow cotton resistance to overhead flooding injury evaluation method, it is characterized in that:
Described submerging treatment method is " two basin (cup) method ", spreads gauze, flat little plastic cup after planting neatly puts in flat large plastic tub, then pour water in the flat large plastic tub of submerging treatment, cover Shamian Island about 1cm to water by bottom opening.
5. according to claim 1 one to grow cotton resistance to overhead flooding injury evaluation method, it is characterized in that:
Described cotton seeds to be measured is unified is light seed.
6. according to claim 1 one to grow cotton resistance to overhead flooding injury evaluation method, it is characterized in that, the concrete evaluation method in step (4) is:
The submerging treatment germination percentage a surveyed is waterflooding 6 d, after planting the germination percentage of 15 d cotton seeds; The contrast germination percentage b surveyed is normal water management, after planting the germination percentage of 15 d cotton seeds;
Described waterlogging index x is relative germination rate, and namely waterlogging index x=submerging treatment germination percentage a/ contrasts germination percentage b × 100%;
If waterlogging index x >=50, cotton variety to be measured is highly waterlogging type cotton variety;
If waterlogging index 30≤x < 50, cotton variety to be measured is waterlogging type cotton variety;
If waterlogging index 10≤x < 30, cotton variety to be measured is intolerant to waterlogging type cotton variety;
If waterlogging index x < 10, cotton variety to be measured is pole intolerant to waterlogging type cotton variety.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105660335A (en) * | 2016-02-16 | 2016-06-15 | 扬州大学 | Method for identifying moisture-resistant barley variety and system |
| CN107046852A (en) * | 2017-04-20 | 2017-08-18 | 湖南省蔬菜研究所 | A kind of method by seed testing capsicum variety resistance to overhead flooding injury |
| CN107258309A (en) * | 2017-07-21 | 2017-10-20 | 邯郸市农业科学院 | A kind of cotton variety annidation, the breeding method of yielding stability |
| CN107750509A (en) * | 2017-09-04 | 2018-03-06 | 江苏沿海地区农业科学研究所 | Barley moisture-proof simple Identification method |
| CN107870226A (en) * | 2017-11-08 | 2018-04-03 | 上海应用技术大学 | Plant resistance to overhead flooding injury evaluation method |
| CN109792929A (en) * | 2019-03-06 | 2019-05-24 | 山东棉花研究中心 | A kind of cotton indoor cultivation method for Ganoderma and its application |
| CN111727862A (en) * | 2020-03-30 | 2020-10-02 | 湖南省棉花科学研究所 | Method for identifying waterlogging tolerance of cotton by using suspension culture technology |
| CN113340820A (en) * | 2021-06-11 | 2021-09-03 | 上海辰山植物园 | Identification method for evaluating waterlogging tolerance of magnolia stelleriana by utilizing photosynthetic property and leaf color |
| CN113412741A (en) * | 2021-06-21 | 2021-09-21 | 中国农业科学院棉花研究所 | Method for identifying resistance of cotton to flooding without top |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105660335A (en) * | 2016-02-16 | 2016-06-15 | 扬州大学 | Method for identifying moisture-resistant barley variety and system |
| CN107046852A (en) * | 2017-04-20 | 2017-08-18 | 湖南省蔬菜研究所 | A kind of method by seed testing capsicum variety resistance to overhead flooding injury |
| CN107258309A (en) * | 2017-07-21 | 2017-10-20 | 邯郸市农业科学院 | A kind of cotton variety annidation, the breeding method of yielding stability |
| CN107750509A (en) * | 2017-09-04 | 2018-03-06 | 江苏沿海地区农业科学研究所 | Barley moisture-proof simple Identification method |
| CN107870226A (en) * | 2017-11-08 | 2018-04-03 | 上海应用技术大学 | Plant resistance to overhead flooding injury evaluation method |
| CN109792929A (en) * | 2019-03-06 | 2019-05-24 | 山东棉花研究中心 | A kind of cotton indoor cultivation method for Ganoderma and its application |
| CN111727862A (en) * | 2020-03-30 | 2020-10-02 | 湖南省棉花科学研究所 | Method for identifying waterlogging tolerance of cotton by using suspension culture technology |
| CN113340820A (en) * | 2021-06-11 | 2021-09-03 | 上海辰山植物园 | Identification method for evaluating waterlogging tolerance of magnolia stelleriana by utilizing photosynthetic property and leaf color |
| CN113340820B (en) * | 2021-06-11 | 2023-01-24 | 上海辰山植物园 | Identification method for evaluating waterlogging tolerance of magnolia stelleriana by utilizing photosynthetic property and leaf color |
| CN113412741A (en) * | 2021-06-21 | 2021-09-21 | 中国农业科学院棉花研究所 | Method for identifying resistance of cotton to flooding without top |
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Application publication date: 20150812 |
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