CN108738733A - A kind of method of Rice Germplasm Resources long-term preservation - Google Patents
A kind of method of Rice Germplasm Resources long-term preservation Download PDFInfo
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- CN108738733A CN108738733A CN201810635717.1A CN201810635717A CN108738733A CN 108738733 A CN108738733 A CN 108738733A CN 201810635717 A CN201810635717 A CN 201810635717A CN 108738733 A CN108738733 A CN 108738733A
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- Prior art keywords
- seed
- storage
- rice
- temperature
- seedling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01F—PROCESSING OF HARVESTED PRODUCE; HAY OR STRAW PRESSES; DEVICES FOR STORING AGRICULTURAL OR HORTICULTURAL PRODUCE
- A01F25/00—Storing agricultural or horticultural produce; Hanging-up harvested fruit
Abstract
The present invention relates to a kind of methods of Rice Germplasm Resources long-term preservation, and steps are as follows：A. it harvests drying；B. the storage of seeds；C. germination situation detects；D. growth of seedling situation is investigated；E. germ plasm resource at any time and timing replace：Germ plasm resource after planting, uses current year seed change to the aged seed of original preservation, or breeding and replacement germ plasm resource as needed.The method of the present invention rice after harvesting, be dried and low-temperature treatment, be conducive to the metabolism for inhibiting seed, it is reduced due to respiration and caused by nutrient consumption, the vigor of seed is maintained, while needing to set reserve temperature according to different, the consumption of the energy can be reduced；A small amount of Rice Cropping resource for the breeder for needing long-term preservation, the method that ultra low temperature freezer can be taken to store, inhibits the respiratory metabolism of seed, seed is made to lie substantially in dormant state, can greatly prolong the Storage of seed.
Description
Technical field
The invention belongs to agricultural product technical fields, are related to the storage of Rice Germplasm Resources, more particularly, to teaching and scientific research
The store method of unit Rice Germplasm Resources, especially a kind of method of Rice Germplasm Resources long-term preservation.
Background technology
Rice is mostly important one of the cereal crops in China, and the quality of rice paddy seed plays certainly the Rice Production in China
Qualitatively effect.With being growing more intense for rice paddy seed competitive market, rice paddy seed operating company to each other in breeding and system
Disordering in terms of kind, often results in the case where current year seed cannot be sold completely, these seeds need safe storage to arrive
Under next year sell.In addition, the rice breeding unit based on research, can often from introducing some germ plasm resources both at home and abroad, with
The continuous of germ plasm resource adds up, and the amount of germ plasm resource can be increasing, all to plant these resource materials every year, need to consume
A large amount of man power and material is practically impossible to realize, thus how long-term preservation these germ plasm resources, when needed can
Take out at any time, and the vigor of seed keep be preferably current the storage of seeds urgent need to resolve the problem of.Previous rice paddy seed storage
Research is often directed to seed production rather than the preservation of breeder's germ plasm resource, especially in accordance with different needs, carries out rice seed
The storage of son is a blank.
Contain a large amount of carbohydrate, that is, starch in rice paddy seed, seed is life entity living, the meeting in respiratory
Much starch is consumed, seed vitality is caused to decline.
Rice paddy seed storage is influenced by holding conditions such as seed moisture content, relative humidity, temperature, suitable storage environment
The vigor for being conducive to keep seed, extends its service life.
Currently, in order to maintain seed vitality, the method for reducing seed moisture content or reserve temperature is usually taken in storage,
But the too low vigor that can influence seed of seed moisture content, and cryopreservation can expend mass energy, increase the cost of storage, be store to seed
Tibetan department and research unit come with larger input, and the present invention specifies the relationship of seed moisture content and the storage of seeds temperature,
Cost can be reduced in actual production, it is simple to operation.
In recent years, more progress is achieved in rice storage research field, but by retrieval, not yet found special with the present invention
Profit applies for relevant patent publication us.
Invention content
Place that the purpose of the present invention is to overcome the deficiency in the prior art, provides a kind of side of Rice Germplasm Resources long-term preservation
Method, this method by reduce seed metabolism and respiration achieve the effect that extend rice paddy seed long-term preservation, solve by
In the reduction of seeds self nutrient caused by respiration causes seed vitality to decline the technical issues of, the method for the present invention is bright
The really relationship of seed moisture content and the storage of seeds temperature, can reduce cost in actual production, simple to operation.
The technical solution adopted by the present invention to solve the technical problems is：
A kind of method of Rice Germplasm Resources long-term preservation, steps are as follows：
A. it harvests drying：It harvests drying after rice maturation, screens full seed, by the moisture control of seed in 7-8%, kind
The proportion > 1 of son；
B. the storage of seeds：Seed full after drying is respectively charged into seed packet, and seed packet is numbered, records seed
Then reserve temperature is set as needed in storage information, specific as follows：
For production seed, store 1 year, temperature is controlled at 15 DEG C or less；For the germ plasm resource of breeder,
It stores 1~2 year, storage temperature-5-- 10 DEG C is set；It stores 5 years, storage temperature-20-- 25 DEG C is set；Storage 10 years
, storage temperature-30-- 35 DEG C is set；Store 10 years or more, -40 DEG C of storage temperature or more；
C. germination situation detects：Germ plasm resource takes out Some seeds and carries out germination feelings when needing to plant or breed
The identification of condition；
D. growth of seedling situation is investigated：Seedling is cultivated, and the growing state of rice seedling is investigated, and evaluates seedling quality；
E. germ plasm resource at any time and timing replace：Germ plasm resource after planting, with current year seed change to originally
The aged seed of preservation, or breeding and replacement germ plasm resource as needed.
Moreover, in the step a, when dry after the seed harvest of rice pest insects material, rate of drying cannot be too fast, uses
The method of natural drying or silica dehydrator.
Moreover, the storage information of seed is kind/be title, storage date in the step b.
Moreover, using low temperature seed bank or low temperature refrigerator when being stored in the step b.
Moreover, the identification of germination includes germination percentage, germinating energy, germination index and vitality index in the step c
Identification.
Moreover, seedling quality investigation includes the investigation of generous basal part of stem, height of seedling, leaf age in the step d.
The advantages of present invention obtains and good effect are：
1, the method for the present invention is after rice harves, is dried and low-temperature treatment, is conducive to the metabolism for inhibiting seed, reduces
Its nutrient consumption caused by due to respiration maintains the vigor of seed, while needing to set reserve temperature according to different, can
The consumption of the energy is reduced, this method reaches the effect for extending rice paddy seed long-term preservation by the metabolism and respiration that reduce seed
Fruit, the technical issues of solving the reduction of seeds self nutrient caused by respiration and seed vitality caused to decline,
Method is simple and practicable；A small amount of Rice Germplasm Resources for the breeder for needing long-term preservation, can take ultra low temperature freezer to store
Method, inhibit the respiratory metabolism of seed, seed made to lie substantially in dormant state, the storage year of seed can be greatly prolonged
Limit；The method of the present invention specifies the relationship of seed moisture content and the storage of seeds temperature, can reduce cost in actual production, letter
It is single easy to operate.
2, the convenience that the method for the present invention is searched for seed is indicated variety name on seed packet, and is carried out to seed packet
Label will carry out record on computer and minute book and put on record, be convenient to use.
3, the germ plasm resource of the method for the present invention after planting, the aged seed preserved originally is replaced with current year new seed, with
Just seed vitality is kept.
Description of the drawings
Fig. 1 is a kind of process flow chart of the method for the present invention.
Specific implementation mode
The embodiment of the present invention is described in detail below, it should be noted that the present embodiment is narrative, is not limited
, protection scope of the present invention cannot be limited with this.
Raw material used in the present invention is unless otherwise specified conventional commercial product；Used in the present invention
Method is unless otherwise specified the conventional method of this field.
Embodiment 1
A kind of method of Rice Germplasm Resources long-term preservation, steps are as follows：
A, it harvests drying, moisture control is in 7-8%, the proportion > 1 of seed after Rice Germplasm Resources drying.
B, the setting of reserve temperature is depending on the length in Germ-plasma resources protection period.It stores 1 year, low temperature seed can be used
Library, temperature are controlled at 15 DEG C or less.It for the germ plasm resource of breeder, stores 1~2 year ,-5-- 10 DEG C of setting is i.e.
It can；It stores 5 years, is arranged-20-- 25 DEG C；It stores 10 years, is arranged-30-- 35 DEG C；Storage 10 years or more, -40 DEG C with
On.
For the convenience that seed is searched, indicate variety name on seed packet, and to seed packet into line label, in computer and
Record will be carried out on minute book to put on record.
C, germ plasm resource takes out the identification that Some seeds carry out germination when needing to plant or breed, and measures germination
The projects such as rate, germinating energy and germination index.
D, seedling culture is carried out to chitting piece, growth of seedling situation is investigated, and the growing state of rice seedling is investigated, and is evaluated
Seedling quality.
E, Rice Germplasm Resources after planting, use current year seed change to the aged seed of original preservation, or according to need
Breed and replace germ plasm resource.
Embodiment 2
A kind of method of Rice Germplasm Resources long-term preservation, steps are as follows：
A. it harvests drying：It harvests drying after rice maturation, screens full seed, by the moisture control of seed in 7-8%, kind
The proportion > 1 of son；
B. the storage of seeds：Seed full after drying is respectively charged into seed packet, and seed packet is numbered, records seed
Then reserve temperature is set as needed in storage information, specific as follows：
For production seed, store 1 year, temperature is controlled at 15 DEG C or less；For the germ plasm resource of breeder,
It stores 1~2 year, storage temperature-5-- 10 DEG C is set；It stores 5 years, storage temperature-20-- 25 DEG C is set；Storage 10 years
, storage temperature-30-- 35 DEG C is set；Store 10 years or more, -40 DEG C of storage temperature or more；
C. germination situation detects：Germ plasm resource takes out Some seeds and carries out germination feelings when needing to plant or breed
The identification of condition；
D. growth of seedling situation is investigated：Seedling is cultivated, and the growing state of rice seedling is investigated, and evaluates seedling quality；
E. germ plasm resource at any time and timing replace：Germ plasm resource after planting, with current year seed change to originally
The aged seed of preservation, or breeding and replacement germ plasm resource as needed.
More preferably, in the step a, when dry after the seed harvest of rice pest insects material, rate of drying cannot be too fast, makes
With natural drying or the method for silica dehydrator.
More preferably, the storage information of seed is kind/be title, storage date in the step b.
More preferably, low temperature seed bank or low temperature refrigerator are used when being stored in the step b.
More preferably, the identification of germination includes that germination percentage, germinating energy, germination index and vigor refer in the step c
Several identifications.
More preferably, in the step d seedling quality investigate include generous basal part of stem, height of seedling, leaf age investigation.
Embodiment 3
As shown in Figure 1, a kind of method of Rice Germplasm Resources long-term preservation, steps are as follows：
A. it harvests drying, is dried to moisture 7~8% after rice maturation, select full rice paddy seed, the proportion > of seed
1；
B., full rice paddy seed after drying is put into -40 DEG C of low temperature refrigerator, storage time was up to 5 years or longer；
C. Germplasms need plantation when, take out Some seeds, carry out germination percentage, germinating energy, germination index and
The measurement of vitality index etc.；
D. after germination, carry out seedling culture, investigate seedling quality, including the basal part of stem scantling of rice shoot, height of seedling and
Leaf age etc.；
E. as needed, Rice Germplasm Resources are periodically or non-periodically replaced, seed vitality is kept.
The coherent detection of Rice Germplasm Resources long-term preservation method of the present invention：
Detect embodiment 1
The variety name of test material is " Tianjin river 1 ", " Tianjin original 45 ", " Tianjin rice 417 ", " rich 47 " of salt, " more light " are planted
Soil types is rice soil, and field management is pressed local cultivation and carried out.It is harvested after October 10 maturation, natural (or silica gel) is dry
So that moisture is down to 7~8%, carries out low-temperature treatment.
Rice Germplasm Resources store investigation result one：
The germination percentage of hundred seeds of data bit in upper table, wherein：
Processing I：Room temperature preservation, temperature change with the variation of outside air temperature, 1 year pot-life；
Processing II：Low temperature seed bank preserves, and temperature setting is 15 DEG C hereinafter, 1 year pot-life；
Processing III：- 5 DEG C of refrigerators preserve, 2 years pot-lives；
Processing IV：- 20 DEG C of refrigerators preserve, 5 years pot-lives；
Processing V：- 40 DEG C of refrigerators preserve, 10 years pot-lives.
Detect embodiment 2
Test material title and storage practice carry out seedling culture with embodiment 1 after germination, measure rice shoot dry weight.
Rice Germplasm Resources store investigation result two：
After upper table is germination, seedling total dry weight measured after cultivating 15 days (average value of unit g), wherein：
Processing I：Room temperature preservation, temperature change with the variation of outside air temperature, 1 year pot-life；
Processing II：Low temperature seed bank preserves, and temperature setting is 15 DEG C hereinafter, 1 year pot-life；
Processing III：- 5 DEG C of refrigerators preserve, 2 years pot-lives；
Processing IV：- 20 DEG C of refrigerators preserve, 5 years pot-lives；
Processing V：- 40 DEG C of refrigerators preserve, 10 years pot-lives.
Detect embodiment 3
Test material title and storage practice carry out seedling culture with embodiment 1 after germination, investigate the flat of each kind leaf age
Mean value.
Rice Germplasm Resources store investigation result three：
Upper table is the average value of investigated seedling leaf age after seedling is cultivated 15 days after germination, wherein：
Processing I：Room temperature preservation, temperature change with the variation of outside air temperature, 1 year pot-life；
Processing II：Low temperature seed bank preserves, and temperature setting is 15 DEG C hereinafter, 1 year pot-life；
Processing III：- 5 DEG C of refrigerators preserve, 2 years pot-lives；
Processing IV：- 20 DEG C of refrigerators preserve, 5 years pot-lives；
Processing V：- 40 DEG C of refrigerators preserve, 10 years pot-lives.
Detect embodiment 4
Test material title and storage practice carry out seedling culture with embodiment 1 after germination, measure single plant seedling root overall length
Average value (root total length/seedling number, the unit of degree：mm).
Rice Germplasm Resources store investigation result four：
Upper table is the average value of the measured total root long (unit mm) of single plant after seedling is cultivated 15 days after germination,
In：
Processing I：Room temperature preservation, temperature change with the variation of outside air temperature, 1 year pot-life；
Processing II：Low temperature seed bank preserves, and temperature setting is 15 DEG C hereinafter, 1 year pot-life；
Processing III：- 5 DEG C of refrigerators preserve, 2 years pot-lives；
Processing IV：- 20 DEG C of refrigerators preserve, 5 years pot-lives；
Processing V：- 40 DEG C of refrigerators preserve, 10 years pot-lives.
It detects in embodiment 1,2,3 and 4, processing I is control, i.e. room temperature is handled, and processing II~V is difference of the invention
The low-temperature treatment of temperature.As can be seen from the test results, although germination percentage, single plant of the different cultivars in different disposal are averagely dry
Weight, leaf age and total root long are different, but all in all, 5 kinds are under the conditions of low-temperature treatment, the germination percentage and rice shoot of seed
Quality is substantially better than room temperature storage and handles, and difference unobvious between the same kind or different cultivars of low-temperature treatment different years.
The part that embodiment 1,2,3 and 4 is this content of the test is detected, from germinating energy, germination index vitality index and seedling
Basal part of stem scantling, leaf age, leaf color all obtain from the point of view of the diameter of root, surface area, diameter, volume, tip of a root number etc. similar
As a result.So the low-temperature treatment time in the present invention complies fully with production seed sale and breeding research unit Germ-plasma resources protection
Index.
Although disclosing the embodiment of the present invention for the purpose of illustration, it will be appreciated by those skilled in the art that：Not
Be detached from the present invention and spirit and scope of the appended claims in, various substitutions, changes and modifications be all it is possible, therefore, this
The range of invention is not limited to embodiment and attached drawing disclosure of that.
Claims (6)
1. a kind of method of Rice Germplasm Resources long-term preservation, it is characterised in that：Steps are as follows：
A. it harvests drying：It harvests drying after rice maturation, screens full seed, the moisture of seed is controlled in 7-8%, seed
Proportion > 1；
B. the storage of seeds：Seed full after drying is respectively charged into seed packet, and seed packet is numbered, records the storage of seed
Then reserve temperature is set as needed in information, specific as follows：
For production seed, store 1 year, temperature is controlled at 15 DEG C or less；For the germ plasm resource of breeder, storage
1~2 year, storage temperature-5-- 10 DEG C is set；It stores 5 years, storage temperature-20-- 25 DEG C is set；It stores 10 years, if
Set storage temperature-30-- 35 DEG C；Store 10 years or more, -40 DEG C of storage temperature or more；
C. germination situation detects：Germ plasm resource takes out Some seeds and carries out germination when needing to plant or breed
Identification；
D. growth of seedling situation is investigated：Seedling is cultivated, and the growing state of rice seedling is investigated, and evaluates seedling quality；
E. germ plasm resource at any time and timing replace：Germ plasm resource after planting, uses current year seed change to original preservation
Aged seed, or as needed breeding and replace germ plasm resource.
2. requiring the effective ways of the Rice Germplasm Resources long-term preservation according to right 1, it is characterised in that：The step a
In, when dry after the seed harvest of rice pest insects material, rate of drying cannot be too fast, uses natural drying or the side of silica dehydrator
Method.
3. requiring the effective ways of the Rice Germplasm Resources long-term preservation according to right 1, it is characterised in that：The step b
The storage information of middle seed is kind/be title, storage date.
4. requiring the effective ways of the Rice Germplasm Resources long-term preservation according to right 1, it is characterised in that：The step b
Low temperature seed bank or low temperature refrigerator are used when middle storage.
5. requiring the effective ways of the Rice Germplasm Resources long-term preservation according to right 1, it is characterised in that：The step c
The identification of middle germination includes the identification of germination percentage, germinating energy, germination index and vitality index.
6. requiring the effective ways of the Rice Germplasm Resources long-term preservation according to right 1, it is characterised in that：The step d
Middle seedling quality investigation includes the investigation of generous basal part of stem, height of seedling, leaf age.
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| CN201810635717.1A CN108738733A (en) | 2018-06-20 | 2018-06-20 | A kind of method of Rice Germplasm Resources long-term preservation |
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| CN201810635717.1A CN108738733A (en) | 2018-06-20 | 2018-06-20 | A kind of method of Rice Germplasm Resources long-term preservation |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114128438A (en) * | 2021-11-26 | 2022-03-04 | 沈阳药科大学 | Method for preserving germplasm resources of shizhu ginseng |
| CN114128437A (en) * | 2021-11-26 | 2022-03-04 | 沈阳药科大学 | Method for preserving sophora flavescens germplasm resources |
| CN114128707A (en) * | 2021-11-26 | 2022-03-04 | 沈阳药科大学 | Schisandra chinensis germplasm resource preservation method |
| CN115363020A (en) * | 2022-10-24 | 2022-11-22 | 中国科学院昆明植物研究所 | Preservation method of bulbil germplasm resources |
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| CN101810071A (en) * | 2010-04-12 | 2010-08-25 | 云南省烟草农业科学研究院 | Storing and breeding method for preventing tobacco germplasm resource from being lost |
| CN101816230A (en) * | 2010-06-01 | 2010-09-01 | 福建农林大学 | Method for producing super-dry rice seeds |
| CN102523846A (en) * | 2012-01-11 | 2012-07-04 | 山东省农作物种质资源中心 | Safety drying method and drying device for long-term seed storage |
| CN102986334A (en) * | 2012-12-13 | 2013-03-27 | 遵义县虾子辣椒专业合作社 | Preservation method of corn seeds |
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2018
- 2018-06-20 CN CN201810635717.1A patent/CN108738733A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810071A (en) * | 2010-04-12 | 2010-08-25 | 云南省烟草农业科学研究院 | Storing and breeding method for preventing tobacco germplasm resource from being lost |
| CN101816230A (en) * | 2010-06-01 | 2010-09-01 | 福建农林大学 | Method for producing super-dry rice seeds |
| CN102523846A (en) * | 2012-01-11 | 2012-07-04 | 山东省农作物种质资源中心 | Safety drying method and drying device for long-term seed storage |
| CN102986334A (en) * | 2012-12-13 | 2013-03-27 | 遵义县虾子辣椒专业合作社 | Preservation method of corn seeds |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114128438A (en) * | 2021-11-26 | 2022-03-04 | 沈阳药科大学 | Method for preserving germplasm resources of shizhu ginseng |
| CN114128437A (en) * | 2021-11-26 | 2022-03-04 | 沈阳药科大学 | Method for preserving sophora flavescens germplasm resources |
| CN114128707A (en) * | 2021-11-26 | 2022-03-04 | 沈阳药科大学 | Schisandra chinensis germplasm resource preservation method |
| CN115363020A (en) * | 2022-10-24 | 2022-11-22 | 中国科学院昆明植物研究所 | Preservation method of bulbil germplasm resources |
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Application publication date: 20181106 |