CN104067726B - The seed seedling-raising method of a kind of birch-leaf pear - Google Patents
The seed seedling-raising method of a kind of birch-leaf pear Download PDFInfo
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- 244000185180 Pyrus betulifolia Species 0.000 title claims abstract description 54
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- 230000008569 process Effects 0.000 claims abstract description 21
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- 239000004576 sand Substances 0.000 claims description 12
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- 238000009423 ventilation Methods 0.000 claims description 8
- 238000009835 boiling Methods 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 6
- 230000004345 fruit ripening Effects 0.000 claims description 6
- 230000008774 maternal effect Effects 0.000 claims description 6
- 229960002523 mercuric chloride Drugs 0.000 claims description 6
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
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- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 5
- 239000002689 soil Substances 0.000 claims description 5
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
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Abstract
The present invention relates to the seed seedling-raising method of a kind of birch-leaf pear, comprise collection seed, soak, and many matrix Stratificated treatment, growth cabinet carry out the steps such as germination cultivation. Adopt the birch-leaf pear seed of the present invention's process, while improving percentage of germination, in turn save the processing costs of seed, solve that seed germination is slow, irregular deficiency of emerging simultaneously, the seeds of nursery difficulty originally are made to breed the simple, quick of change, more easily promoting in production, percentage of germination reaches more than 95%.
Description
Technical field
The present invention relates to agriculture field, what be specifically related to is the seed seedling-raising method of a kind of birch-leaf pear.
Background technology
Birch-leaf pear (PyrusbetulifoliaBunge) Rosaceae pears belong to deciduous tree, and branch often has thorn. Strain is high 10 meters, and branch tool stings. Leaf water chestnut shape ovum type is to oval type, and umbrella shape raceme, spends 10��15, petal white, style 2��3. Fruit is closely spherical, brown, April at florescence, really 8��September of phase. The anti-arid of birch-leaf pear, resistance to cold and cool, usually make the stock of various cultivation pears, the result phase, early the life-span was very long. Timber densification can make various implements. Bark, containing tannin, can be obtained through refining tannin extract and be used as medicine.
In pear tree cultivation and breeding, substantially adopting graft seedling growth, also just says, first sowing stock seed, cultivates into the rootstock seedling that grows directly from seeds, then grafting cultivation kind thereon. The Cultivar of the kinds such as birch-leaf pear and white pear, Chinese pear, Ussurian pear all has very strong affinity, and has cold-resistant, drought resisting, the characteristic such as saline and alkaline waterlogging, resistance to, is therefore that China uses maximum pear tree stocks at present. But, birch-leaf pear seed is directly sowed and can not be germinateed, in production, generally before sowing, seed is carried out Stratificated treatment, so-called Stratificated treatment mix by seed with the wet matters such as wet sand or wet peat or when layering is placed on low temperature, moistening, ventilation after storage 40��60 days breaking dormancies, sows ability rudiment. Seed often occurs to go rotten because moisture or aeration condition hold not good during sand is hidden, or hold the length of not good cool stratification time, thus affect the normal breaking dormancy of seed, cause sowing rear seed can not germinate or percentage of germination low, make ready field cannot normally nursery and missed nursery, cause produce season affect adversely and financial loss, for by hybridization regain cenospecies, if seed Stratificated treatment can not germinate well, breeding work plan will be affected.
Summary of the invention
The present invention is directed to the seed seedling-raising method that existing deficiency provides a kind of birch-leaf pear, present method can improve the survival rate of seedling of birch-leaf pear seed.
In order to realize above-mentioned task, the present invention takes following technical solution:
A seed seedling-raising method for birch-leaf pear, comprises the following steps:
(1) in birch-leaf pear fruit maturation season, good from result performance, contain without the result of disease and pest and phase maternal plant gathers fruit is big, look gorgeous, seed is full, shine 3-4d without the fruit of disease and pest, after skin softness, peel off skin and flesh and be used as to plant;
(2) sterilize, soak seed: the seed of collection is spread at the shady and cool place of ventilation, it is that 0.1��0.2% mercuric chloride solution soaks 5-7min sterilization by the seed mass volume ratio gathered after shady dry 3d, then seed is rinsed with clear water, after seed is poured in 100 DEG C of boiling water, constantly stir, until water temperature is down to less than 50 DEG C, then add lid, water soaks 24h, naturally cooling;
(3) cheat Tibetan and macerate kind: first the seed that step (2) obtains is placed on clear water resume and soaks 4-6 days, then after admixing the plant ash containing plant-growth regulator in above-mentioned seed, digging that half meter of dell is vexed to be macerated, the bottom in hole spreads one layer, and to macerate ripe pig excrement 5��10cm thick, then it is thick to spread one layer of sand 1��3cm, then one layer of seed is spread, Using such method one layer of pig excrement one layer of sand, one layer of seed, stacks 5-7 layer, then covers the thick sandy soil of 20cm, keep moistening, bury 10��15 days;
(4) then the birch-leaf pear seed through above-mentioned process is cultivated 5��8 days on germinating bed, temperature is 5��10 DEG C, and then move into growth cabinet carry out germinate cultivate, every day adds appropriate amounts of sterilized water, to keep, filter paper is moistening is as the criterion for add-on, water filling wants evenly consistent, makes Seed imbibes good, carries out altogether 20��30 days; From the 8th day, observe whether seed has breach, within every 2 days, observe once, when 2/3 in seed has breach, for sowing.
Described plant-growth regulator is: 6-BA110-130ppm, naphthylacetic acid 70-80ppm, surplus are water.
Germinating bed described in step (4) is the clean culture dish of diameter 30cm, is encased inside double-layer circular filter paper and soaks into the potassium dihydrogen phosphate of 0.5% in described culture dish.
The illumination condition that described growth cabinet carries out germination cultivation is set as that illumination period every day, dark period hocket: light application time 12��16 hours, temperature 28 DEG C, and light intensity is 3000��5000LX; Dark 8��12 hours time, temperature is 18 DEG C, and light intensity is 150��800LX.
According to the present invention method process after birch-leaf pear seed, its germinating energy and percentage of germination are measured, its method respectively:
1) germinating energy be carry out rate of emergence detection the initial stage carry out, the birch-leaf pear seed of the present invention started at 5th��12 days to sprout, and accounts for for the per-cent planting experimentally son with the 8th day normal seed number germinateed of numeration for the first time, represents with %, being accurate to 0.1%, calculation formula is as follows:
GE=N1/ N �� 100%
In formula: GE represents germinating energy, %; N represents for planting experimentally subnumber; N1Represent the seed number all normally germinateed in regulation number of days;
2) detection of percentage of germination calculates the seed number germinateed in the number of days of regulation under seed is placed on optimum germination condition, and calculates the percentage accounted for for planting experimentally son, represents with %, is accurate to 0.1%; Calculation formula is as follows:
Percentage of germination=regulation percentage of germination measures the normal kind seed number/confession germinateed in number of days and plants experimentally seed number * 100%
After first numeration, every numeration in 1��2 day once, record meets the normal seedling of Seed Inspection norm standard, obviously dead or rotten seed is taken out and counts; 30th heaven-made last numeration, records all normal seedlings, abnormal seedling, hardseed, fresh non-chitting piece and blind seed number respectively, and composite grain seed units produces the normal seedling of a more than strain, only records a strain seedling;
Carrying out the method for birch-leaf pear seed seedling-raising according to the method for the present invention, birch-leaf pear seed, average germination percentage can reach more than 95%, and seedling raise period shortens greatly.
The present invention has the following advantages:
1, invention comprehensive utilization immersion, growth regulator adjustment and many matrix Stratificated treatment etc. break the technology of seed dormancy, the technology of particularly boiling water immersion, clear water immersion and many matrix Stratificated treatment, comprise and have employed multilayer matrix so that utilize the seedling from seed well developed root system of the birch-leaf pear that the present invention obtains;
2, the birch-leaf pear seed of the present invention's process is adopted, improving while percentage of germination, in turn save the processing costs of seed, solve that seed germination is slow, irregular deficiency of emerging simultaneously, make the seeds of nursery difficulty originally breed the simple, quick of change, more easily promote in production;
3, the lamination matrix that the present invention prepares, adds the ventilation property of matrix, and then is conducive to the breathing of seed, therefore, accelerates the broken dormancy process of birch-leaf pear seed;
4, the present invention adopts and cultivates in conjunction with the mode that growth cabinet is cultivated on germinating bed, and artificial its culture condition of manipulation, percentage of germination is further enhanced.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, and the present invention, only for illustration of the present invention, is not limited by following each embodiment.
Embodiment 1
A seed seedling-raising method for birch-leaf pear, comprises the following steps:
(1) in birch-leaf pear fruit maturation season, good from result performance, contain without the result of disease and pest and phase maternal plant gathers fruit is big, look gorgeous, seed is full, slightly shine 3d without the fruit of disease and pest, after skin softness, peel off skin and flesh and be used as to plant;
(2) sterilize, soak seed: the seed of collection is spread at the shady and cool place of ventilation, it is that 0.1% mercuric chloride solution soaks 7min sterilization by the seed mass volume ratio gathered after shady dry 3d, then seed is rinsed with clear water, after seed is poured in 100 DEG C of boiling water, constantly stir, until water temperature is down to less than 50 DEG C, then add lid, water soaks 24h, naturally cooling;
(3) cheat Tibetan and macerate kind: first the seed that step (2) obtains is placed on clear water resume and soaks 4 days, then after admixing the plant ash containing plant-growth regulator (6-BA110ppm, naphthylacetic acid 80ppm, surplus are water) in above-mentioned seed, dig that half meter of dell is vexed to be macerated, bottom in hole spreads one layer, and to macerate ripe pig excrement 5cm thick, spread one layer of sand 3cm thicker, then one layer of seed is spread, Using such method one layer of pig excrement one layer of sand, one layer of seed, stack 5 layers, cover the sandy soil that 20cm is thick again, keep moistening, bury 10 days;
(4) then the birch-leaf pear seed through above-mentioned process is cultivated 5 days on germinating bed, temperature is 10 DEG C, and then move into growth cabinet carry out germinate cultivate, every day adds appropriate amounts of sterilized water, to keep, filter paper is moistening is as the criterion for add-on, water filling wants evenly consistent, makes Seed imbibes good, carries out altogether 30 days; From the 8th day, observe whether seed has breach, within every 2 days, observe once, when 2/3 in seed has breach, for sowing.
Germinating bed described in step (4) is the clean culture dish of diameter 30cm, is encased inside double-layer circular filter paper and soaks into the potassium dihydrogen phosphate of 0.5% in described culture dish.
The illumination condition that described growth cabinet carries out germination cultivation is set as that illumination period every day, dark period hocket: light application time 12 hours, temperature 28 DEG C, and light intensity is 5000LX; The dark 12 hours time, temperature is 18 DEG C, and light intensity is 150LX.
The birch-leaf pear seed processed according to the method described above, measures its germinating energy and percentage of germination, its method respectively:
1) germinating energy be carry out rate of emergence detection the initial stage carry out, the birch-leaf pear seed of the present invention started at 5th��12 days to sprout, and accounts for for the per-cent planting experimentally son with the 8th day normal seed number germinateed of numeration for the first time, represents with %, being accurate to 0.1%, calculation formula is as follows:
GE=N1/ N �� 100%
In formula: GE represents germinating energy, %; N represents for planting experimentally subnumber; N1Represent the seed number all normally germinateed in regulation number of days;
2) detection of percentage of germination calculates the seed number germinateed in the number of days of regulation under seed is placed on optimum germination condition, and calculates the percentage accounted for for planting experimentally son, represents with %, is accurate to 0.1%; Calculation formula is as follows:
Percentage of germination=regulation percentage of germination measures the normal kind seed number/confession germinateed in number of days and plants experimentally seed number * 100%
After first numeration, every numeration in 1��2 day once, record meets the normal seedling of Seed Inspection norm standard, obviously dead or rotten seed is taken out and counts; 30th heaven-made last numeration, records all normal seedlings, abnormal seedling, hardseed, fresh non-chitting piece and blind seed number respectively, and composite grain seed units produces the normal seedling of a more than strain, only records a strain seedling.
Its detection being carried out 4 times repeat, the seed number every time chosen is 100, then calculates the mean number repeated for 4 times, and in table 1, the birch-leaf pear rate of emergence showing that this test draws by calculating mean number is 95%.
The measurement result of table 1 birch-leaf pear rate of emergence
| Repeat | First record | First counting chitting piece number | Last record | Last counting chitting piece number | Percentage of germination |
| 1 | 10th day | 12 | 30th day | 95 | 95% |
| 2 | 12nd day | 15 | 30th day | 93 | 93% |
| 3 | 14th day | 30 | 30th day | 95 | 95% |
| 4 | 16th day | 42 | 30th day | 97 | 97% |
Comparative example 1
The seed of birch-leaf pear is through following process:
(1) in birch-leaf pear fruit maturation season, good from result performance, contain without the result of disease and pest and phase maternal plant gathers fruit is big, look gorgeous, seed is full, slightly shine 3d without the fruit of disease and pest, after skin softness, peel off skin and flesh and be used as to plant;
(2) sterilize, soak seed: the seed of collection is spread at the shady and cool place of ventilation, it is that 0.1% mercuric chloride solution soaks 7min sterilization by the seed mass volume ratio gathered after shady dry 3d, then seed is rinsed with clear water, after seed is poured in 100 DEG C of boiling water, constantly stir, until water temperature is down to less than 50 DEG C, then add lid, water soaks 24h, naturally cooling.
Without the process of the step (3) in embodiment 1.
Then being cultivated on germinating bed 5 days by the birch-leaf pear seed through above-mentioned process, temperature is 10 DEG C, and then move into growth cabinet carry out germinate cultivate, every day adds appropriate amounts of sterilized water, to keep, filter paper is moistening is as the criterion for add-on, and water filling wants evenly consistent, make Seed imbibes good.
Germinating bed described in step (4) is the clean culture dish of diameter 30cm, is encased inside double-layer circular filter paper and soaks into the potassium dihydrogen phosphate of 0.5% in described culture dish.
The illumination condition that described growth cabinet carries out germination cultivation is set as that illumination period every day, dark period hocket: light application time 12 hours, temperature 28 DEG C, and light intensity is 5000LX; The dark 12 hours time, temperature is 18 DEG C, and light intensity is 150LX.
Being detected by the percentage of germination of its seed, arrange 4 repetitions equally, each repeats to select 100 seeds, and the percentage of germination of its seed is in table 2.
Table 2 is hidden without hole and is macerated kind of a birch-leaf pear rate of emergence for process
| Repeat | First record | First counting chitting piece number | Last record | Last counting chitting piece number | Percentage of germination |
| 1 | 10th day | 0 | 30th day | 0 | 0 |
| 2 | 12nd day | 0 | 30th day | 0 | 0 |
| 3 | 14th day | 0 | 30th day | 0 | 0 |
| 4 | 16th day | 0 | 30th day | 0 | 0 |
Visible, the seed newly gathered in contrast 2 does not process through the broken dormancy of seed, and seed is also in resting stage, can not germinate after sowing.
Comparative example 2
The seed of birch-leaf pear is through following process:
Step (1) is with the step (1) in embodiment 1.
(2) nursery is carried out after naturally being placed 3 months by seed.
Step (3), (4) are with the step (3) in embodiment 1, (4).
The naturally broken dormancy of table 3 birch-leaf pear rate of emergence after 3 months
| Repeat | First record | First counting chitting piece number | Last record | Last counting chitting piece number | Percentage of germination |
| 1 | 10th day | 0 | 30th day | 9 | 9% |
| 2 | 12nd day | 0 | 30th day | 8 | 8% |
| 3 | 14th day | 2 | 30th day | 10 | 10% |
| 4 | 16th day | 5 | 30th day | 11 | 11% |
Naturally broken dormancy after 3 months birch-leaf pear seed average germination percentage be 9.5%, this is because seed does not also have complete breaking dormancy.
Comparative example 3
The seed of birch-leaf pear is through following process:
Step (1) is with the step (1) in embodiment 1.
(2) nursery is carried out after naturally being placed 1 year by seed.
Step (3), (4) are with the step (3) in embodiment 1, (4).
The naturally broken dormancy of table 4 birch-leaf pear rate of emergence after a year
| Repeat | First record | First counting chitting piece number | Last record | Last counting chitting piece number | Percentage of germination |
| 1 | 10th day | 10 | 30th day | 78 | 78% |
| 2 | 12nd day | 18 | 30th day | 73 | 73% |
| 3 | 14th day | 29 | 30th day | 71 | 71% |
| 4 | 16th day | 39 | 30th day | 80 | 80% |
Learn, after 1 year birch-leaf pear seed substantially all completed the broken dormancy of nature after 1 year birch-leaf pear seed be substantially completed the broken dormancy of nature, its average germination percentage is 76.5%.
Can learning from the contrast of embodiment 1 with comparative example 1,2,3, the present invention is after the broken dormancy of seed processes, and the time shifts to an earlier date greatly, and percentage of germination increases greatly.
Embodiment 2
The seed of birch-leaf pear is through following process:
(1) in birch-leaf pear fruit maturation season, good from result performance, contain without the result of disease and pest and phase maternal plant gathers fruit is big, look gorgeous, seed is full, slightly shine 4d without the fruit of disease and pest, after skin softness, peel off skin and flesh and be used as to plant;
(2) sterilize, soak seed: the seed of collection is spread at the shady and cool place of ventilation, it is that 0.2% mercuric chloride solution soaks 5min sterilization by the seed mass volume ratio gathered after shady dry 3d, then seed is rinsed with clear water, after seed is poured in 100 DEG C of boiling water, constantly stir, until water temperature is down to less than 50 DEG C, then add lid, water soaks 24h, naturally cooling;
(3) cheat Tibetan and macerate kind: first the seed that step (2) obtains is placed on clear water resume and soaks 6 days, then after admixing the plant ash containing plant-growth regulator in above-mentioned seed, digging that half meter of dell is vexed to be macerated, the bottom in hole spreads one layer, and to macerate ripe pig excrement 10cm thick, then it is thick to spread one layer of sand 2cm, then one layer of seed is spread, Using such method one layer of pig excrement one layer of sand, one layer of seed, stacks 7 layers, then covers the thick sandy soil of 20cm, keep moistening, bury 15 days;
(4) then the birch-leaf pear seed through above-mentioned process is cultivated 8 days on germinating bed, temperature is 5 DEG C, and then move into growth cabinet carry out germinate cultivate, every day adds appropriate amounts of sterilized water, to keep, filter paper is moistening is as the criterion for add-on, water filling wants evenly consistent, makes Seed imbibes good, carries out altogether 30 days; From the 8th day, observe whether seed has breach, within every 2 days, observe once, when 2/3 in seed has breach, for sowing.
Described plant-growth regulator is: 6-BA130ppm, naphthylacetic acid 70ppm, surplus are water.
Germinating bed described in step (4) is the clean culture dish of diameter 30cm, is encased inside double-layer circular filter paper and soaks into the potassium dihydrogen phosphate of 0.5% in described culture dish.
The illumination condition that described growth cabinet carries out germination cultivation is set as that illumination period every day, dark period hocket: light application time 16 hours, temperature 28 DEG C, and light intensity is 3000LX; The dark 8 hours time, temperature is 18 DEG C, and light intensity is 800LX.
Its detection being carried out 4 times repeat, the seed number every time chosen is 100, then calculates the mean number repeated for 4 times, and the birch-leaf pear rate of emergence showing that this test draws by calculating mean number is 96%, specifically in table 5.
The measurement result of table 5 birch-leaf pear rate of emergence
| Repeat | First record | First counting chitting piece number | Last record | Last counting chitting piece number | Percentage of germination |
| 1 | 10th day | 15 | 30th day | 96 | 96% |
| 2 | 12nd day | 20 | 30th day | 94 | 94% |
| 3 | 14th day | 34 | 30th day | 96 | 96% |
| 4 | 16th day | 48 | 30th day | 98 | 98% |
Comparative example 4
The seed of birch-leaf pear is through following process:
(1) in birch-leaf pear fruit maturation season, good from result performance, contain without the result of disease and pest and phase maternal plant gathers fruit is big, look gorgeous, seed is full, slightly shine 4d without the fruit of disease and pest, after skin softness, peel off skin and flesh and be used as to plant;
(2) sterilize, soak seed: the seed of collection is spread at the shady and cool place of ventilation, it is that 0.2% mercuric chloride solution soaks 5min sterilization by the seed mass volume ratio gathered after shady dry 3d, then seed is rinsed with clear water, after seed is poured in 100 DEG C of boiling water, constantly stir, until water temperature is down to less than 50 DEG C, then add lid, water soaks 24h, naturally cooling;
(3) cheat Tibetan and macerate kind: first the seed that step (2) obtains is placed on clear water resume and soaks 6 days, then after admixing the plant ash containing plant-growth regulator in above-mentioned seed, digging that half meter of dell is vexed to be macerated, the bottom in hole spreads one layer, and to macerate ripe pig excrement 10cm thick, then it is thick to spread one layer of sand 2cm, then one layer of seed is spread, Using such method one layer of pig excrement one layer of sand, one layer of seed, stacks 7 layers, then covers the thick sandy soil of 20cm, keep moistening, bury 15 days;
(4) then being cultivated on germinating bed 8 days by the birch-leaf pear seed through above-mentioned process, temperature is 5 DEG C, then cultivates 30 days on germinating bed always, from the 8th day, observe whether seed has breach, within every 2 days, observe once, when 2/3 in seed has breach, for sowing.
Described plant-growth regulator is: 6-BA130ppm, naphthylacetic acid 70ppm, surplus are water.
Germinating bed described in step (4) is the clean culture dish of diameter 30cm, is encased inside double-layer circular filter paper and soaks into the potassium dihydrogen phosphate of 0.5% in described culture dish.
It is do not transfer to growth cabinet to carry out cultivation of germinateing with the difference of embodiment 1.
Being detected by the percentage of germination of its seed, arrange 4 repetitions equally, each repeats to select 100 seeds, and the percentage of germination of its seed is in table 6, and the average germination percentage calculated is 81.5%.
Table 6 carries out the birch-leaf pear rate of emergence of germination cultivation process without growth cabinet
| Repeat | First record | First counting chitting piece number | Last record | Last counting chitting piece number | Percentage of germination |
| 1 | 10th day | 8 | 30th day | 78 | 78% |
| 2 | 12nd day | 12 | 30th day | 82 | 82% |
| 3 | 14th day | 18 | 30th day | 86 | 86% |
| 4 | 16th day | 20 | 30th day | 80 | 80% |
From the contrast of embodiment 2 and comparative example 6 it may be seen that carry out germination cultivation process growth cabinet, it is possible to improve its percentage of germination.
To sum up, being macerated kind by Tibetan, hole, carry out the aggregate measures such as germination cultivation process in growth cabinet, the percentage of germination of birch-leaf pear seed improves greatly.
Embodiment recited above is only the preferred embodiment of the present invention be described, and not the spirit and scope of the present invention is limited. The content of request of the present invention protection is all recorded in detail in the claims.
Claims (2)
1. the seed seedling-raising method of a birch-leaf pear, it is characterised in that, comprise the following steps:
(1) in birch-leaf pear fruit maturation season, good from result performance, contain without the result of disease and pest and phase maternal plant gathers fruit is big, look gorgeous, seed is full, shine 3-4d without the fruit of disease and pest, after skin softness, peel off skin and flesh and be used as to plant;
(2) sterilize, soak seed: the seed of collection is spread at the shady and cool place of ventilation, it is that 0.1��0.2% mercuric chloride solution soaks 5-7min sterilization by the seed mass volume ratio gathered after shady dry 3d, then seed is rinsed with clear water, after seed is poured in 100 DEG C of boiling water, constantly stir, until water temperature is down to less than 50 DEG C, then add lid, water soaks 24h, naturally cooling;
(3) cheat Tibetan and macerate kind: first the seed that step (2) obtains is placed on clear water resume and soaks 4-6 days, then after admixing the plant ash containing plant-growth regulator in above-mentioned seed, digging that half meter of dell is vexed to be macerated, the bottom in hole spreads one layer, and to macerate ripe pig excrement 5��10cm thick, then it is thick to spread one layer of sand 1��3cm, then one layer of seed is spread, Using such method one layer of pig excrement one layer of sand, one layer of seed, stacks 5-7 layer, then covers the thick sandy soil of 20cm, keep moistening, bury 10��15 days; Described plant-growth regulator is: 6-BA110-130ppm, naphthylacetic acid 70-80ppm, surplus are water;
(4) then the birch-leaf pear seed through above-mentioned process is cultivated 5��8 days on germinating bed, temperature is 5��10 DEG C, and then move into growth cabinet carry out germinate cultivate, every day adds appropriate amounts of sterilized water, to keep, filter paper is moistening is as the criterion for add-on, water filling wants evenly consistent, makes Seed imbibes good, carries out altogether 20��30 days; From the 8th day, observe whether seed has breach, within every 2 days, observe once, when 2/3 in seed has breach, for sowing; The illumination condition that described growth cabinet carries out germination cultivation is set as that illumination period every day, dark period hocket: light application time 12��16 hours, temperature 28 DEG C, and light intensity is 3000��5000LX; Dark 8��12 hours time, temperature is 18 DEG C, and light intensity is 150��800LX.
2. the seed seedling-raising method of a kind of birch-leaf pear as claimed in claim 1, it is characterized in that, germinating bed described in step (4) is the clean culture dish of diameter 30cm, is encased inside double-layer circular filter paper and soaks into the potassium dihydrogen phosphate of 0.5% in described culture dish.
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| CN105830583A (en) * | 2015-04-13 | 2016-08-10 | 王小兵 | Method for rapid breaking of Pyrus betulifolia Bunge seed dormancy and promotion of germination |
| CN106068778A (en) * | 2016-06-14 | 2016-11-09 | 中国农业科学院果树研究所 | A kind of hupehensis Rehd and Mains micromalus seed freezer lamination method |
| CN106879276A (en) * | 2017-03-17 | 2017-06-23 | 界首市伟盛家庭农场 | A kind of Seed Treatment method for culturing seedlings for strengthening pear tree preventing from heavy metal characteristic |
| CN107223381A (en) * | 2017-05-17 | 2017-10-03 | 何子顺 | A kind of quick method for producing birch-leaf pear seed |
| CN107548849A (en) * | 2017-09-26 | 2018-01-09 | 安徽徽大农业有限公司 | A kind of seed seedling-raising method of Cuiguan pear |
| CN107466790A (en) * | 2017-10-13 | 2017-12-15 | 太湖县香茗山生态果品科技园有限公司 | Pear tree stock seed fast breeding method |
| CN111053033A (en) * | 2019-12-10 | 2020-04-24 | 江苏省农业科学院 | Annual breeding method of pear salt-tolerant rootstocks |
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| CN101563969A (en) * | 2009-06-05 | 2009-10-28 | 南京农业大学 | Method for quickly breaking dormancy of birch-leaf pear seeds with hydroxylamine chloride |
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| CN103430656A (en) * | 2013-08-08 | 2013-12-11 | 河南科技大学 | Seedling culture method capable of relieving pear seeds from dormancy |
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| CN101563969A (en) * | 2009-06-05 | 2009-10-28 | 南京农业大学 | Method for quickly breaking dormancy of birch-leaf pear seeds with hydroxylamine chloride |
| CN101904236A (en) * | 2010-06-02 | 2010-12-08 | 南京农业大学 | The Method of Accelerating Germination to Quickly Release the Dormancy of Duli Seeds by Using Isothioguanidinium |
| CN103430656A (en) * | 2013-08-08 | 2013-12-11 | 河南科技大学 | Seedling culture method capable of relieving pear seeds from dormancy |
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