CN104737670B - A kind of method for culturing seedlings of oil seed of Paeonia suffruticosa - Google Patents

A kind of method for culturing seedlings of oil seed of Paeonia suffruticosa Download PDF

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CN104737670B
CN104737670B CN201510196728.0A CN201510196728A CN104737670B CN 104737670 B CN104737670 B CN 104737670B CN 201510196728 A CN201510196728 A CN 201510196728A CN 104737670 B CN104737670 B CN 104737670B
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seed
oil
paeonia suffruticosa
husky
seedling
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CN201510196728.0A
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CN104737670A (en
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戢小梅
陈法志
陈镇
童正富
杨阳
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武汉市林业果树科学研究所
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Abstract

The invention belongs to botanical seedling culturing technical field, disclose a kind of oil seeding growing seedlings method of Paeonia suffruticosa, the method includes on the collection of oil seed of Paeonia suffruticosa and process, the storage of seeds, seedbed preparation, seeding and seedling raising, shifting bud after bag, transplanting the measures such as management.Compared with prior art, the present invention has that the seed treatment time is short, method for treating seeds simple, it is neat to emerge, emergence rate is high, planting percent is high, seedling cost is low, transplants not by advantages such as seasonal effect after seedling.

Description

A kind of method for culturing seedlings of oil seed of Paeonia suffruticosa
Technical field
The invention belongs to botanical seedling culturing technical field, relate to a kind of method for culturing seedlings, more particularly, the present invention relate to Oil seed of Paeonia suffruticosa method for culturing seedlings.
Background technology
Recently as economy and the quick raising of living standards of the people, the demand of edible oil is raised by China year by year, The supply of domestic edible oil is the most not enough, and according to relevant statistics, the 60% of China's edible oil total amount depends on import, alreadys more than International edible oil safe early warning line.At present, the main oil crop Semen sojae atricolor of China, rely on substantially import.Meanwhile, China Oil crop are mainly herbaceous plant, and land resource is limited, and the growth space of cultivated area and yield is the least.In this background Under, the distinctive new good quality and high output oil crop of Devoting Major Efforts To Developing China are extremely urgent.Oil Paeonia suffruticosa (Paeonia Suffruticosa Andr.for oil) it is the exclusive initial species plant of China, it is Paeoniaceae Paeonia sect. Moutan plant produces The general designation of the kind of seed oil yield high (more than or equal to 22%).The oil content of oil seed of Flos Moutan can reach 24.12%-37.83%, The content of unsaturated fatty acid can reach 92.0%, and the alpha-linolenic acid content being wherein referred to as " plant NAOHUANGJIN " is 41.86%, Higher than edible oil such as Camellia oil, walnut oil, olive oil, Oleum Helianthi, Oleum Arachidis hypogaeae semen and Oleum sesami, it it is more than 140 times of olive oil.This Outward, peony seed oil is possibly together with the nutrient substance such as distinctive paeonol, Paeonia suffruticosa Saponin, Paeonia suffruticosa polysaccharide, 24-ethylidenecholest-5-en-3.beta.-ol., Squalene, tool There are the effects such as prevention cardiovascular disease, blood fat reducing, blood pressure lowering, anti-inflammation and sterilization, activating cell, defying age, cancer-resisting.2011 March 22, No. 9 publication approval peony seed oil of Ministry of Health of the People's Republic of China, as new resource food, indicates seed of Flos Moutan Oil has become as the high-grade edible vegetable oil of " legal ", enters edible oil ranks.Oil uses Paeonia suffruticosa as the distinctive ecological wooden of China These oil crop, either from the strategic concerns that the nutritive value of itself still stands in country's safe overall situation of grain and oil, it is all There is great Development volue and market prospect.At present, oil is entered by the method for the commonly used seeding and seedling raising of research worker with Paeonia suffruticosa Row nursery, resource conservation and breeding expanding propagation, therefore research oil Paeonia suffruticosa new technique in terms of seeding and seedling raising and seed germination, right In oil further investigation and the industrialization development of Paeonia suffruticosa, there is realistic meaning.
The oil proliferation of Paeonia suffruticosa, up to the present has no that particular study is reported.Go through in the oil cultivation of Paeonia suffruticosa Cheng Zhong, modal propagation method is sowed exactly, but owing to oil is thicker harder by Paeonia suffruticosa seed coat waxiness, sprouting is taken root the most tired Difficulty, up to the present prior art also is not difficult to sprout the simple and effective place of problem proposition taken root for oil seed of Paeonia suffruticosa Reason method.Meanwhile, oil seed of Paeonia suffruticosa there is also epicotyl or plumule dormancy, and seed germination is irregular, some seed The most do not germinate in several years and be constantly in resting state.Oil uses Paeonia suffruticosa industry development by national and local attention with big in recent years Power is supported, the expansion that its research is the most like a raging fire with industry development.While it is true, the oil of China by Paeonia suffruticosa industry still in starting In the stage, the research of the aspects such as seedling-raising technique is still the problem needing solution badly.Sowing in the field nursery mainly taked by oil with Paeonia suffruticosa at present Mode, emergence rate, regularity and go out garden and transplant and be all affected, cause that production efficiency is low, labour force and the loss of the means of production, This oil supply breed breeding of Paeonia suffruticosa, the research of germ plasm resource bring the biggest difficulty, therefore with protection work, industrialization development etc. The most in the urgent need to address is effectively to break oil seed of Paeonia suffruticosa dormancy and improve breeding breeding efficiency, nursery regularity and become The method of Seedling rate.
Additionally, the oil root system of Paeonia suffruticosa belongs to meat root system, being easily subject to damage, root system comes to harm and is directly connected to move Plant survival rate.In order to be effectively improved the oil transplanting survival rate of Paeonia suffruticosa seedling, it is necessary to use certain method seedlings root to be moved Plant injured probability to be preferably minimized.Although the mode of tradition sowing in the field can avoid oil to cause root because of transplanting with Paeonia suffruticosa seedling It is injured, but tradition type of seeding is hardly formed standardization, standardization, intensive manufacture, is accompanied by seedling-growing time simultaneously Long, irregular, the problem such as emergence rate is low of emerging, is unfavorable for the oil industrialized development of Paeonia suffruticosa.
In recent years, due to the sowing in the field technology that use, the oil transplanting work of Paeonia suffruticosa seedling is typically all after planting more The nine of Second Year, the October carry out, on the one hand easily root system is damaged, on the other hand planting of transplanted seedling is also easy to Limited by season, be unfavorable for the demand of industrialized development.
Finding according to retrieval, " Shaanxi agricultural sciences " the 6th phase in 2011 discloses Paeonia papaveracea (purple butterfly is windward) sowing and educates Seedling-growing method is studied, and uses the method that fresh seeds is live, during sowing, by thin for soil deep tillage rake, opens the deep ditch of 10cm with hoe, will Seed is spread on bottom of trench, rakes with rake, and the cycle of emerging is 30 weeks, and emergence rate is 49%.The land for growing field crops of this kind of method employing is straight Broadcasting method, the cycle of emerging is long, and emergence rate is low.
CN201010569561.5 (entitled " a kind of seed of Paeonia suffruticosa container for plant growth and the fast seedling growing side of this container of employing Method ") disclose a kind of seed of Paeonia suffruticosa container for plant growth and use the fast seedling-cultivating method of this container, comprise the following steps: 1) test Material: Paeonia delavayi seed;2) container for plant growth: it is raw that described container for plant growth includes that sidepiece and bottom, described sidepiece and/or bottom use Thing degradability non-woven fabrics prepares, and cylindrically, height is 7cm to 12cm, a diameter of 4cm to 8cm of upper opening, and described Ratio between diameter and the diameter of bottom of upper opening is 1: 0.8 to 1.2, and the density of non-woven fabrics is 10g/m2To 40g/m2, The retentiveness of non-woven fabrics is 140g/m2To 200g/m2;3) processing method of seed of Paeonia suffruticosa: initially with 0.1 weight % to 0.2 weight The potassium permanganate of amount % is soaked seed 2 hours to 3 hours, rinses with clear water, the hot-water soak seed using temperature to be 55 DEG C to 80 DEG C Within 10 minutes to 15 minutes, seed is carried out sterilizing, then uses the seed soaking that seed of Paeonia suffruticosa is carried out 3 days to 5 days by normal-temperature water, The root-inducing powder aqueous solution finally using 40mg/ml to 60mg/ml is soaked seed 12 hours to 24 hours.4) seeding growing seedlings method: to male Red seed carries out sterilizing and/or seed soaking, sows seed of Paeonia suffruticosa and carry out in greenhouse in equipped with the non-woven container of substrate Root culture, sows 3 for every bag, and substrate is Vermiculitum and the mixture of the peat composed of rotten mosses of volume ratio 2.5-3.5:1, and the water content of substrate is protected Holding in 30% weight to 80% weight, depth of planting is 2.0-2.5Cm, and greenhouse temperature is 15-25 DEG C, and incubation time is 8-12 In week, processing 6-12 week breaking dormancy under conditions of low temperature 2-6 DEG C, rooting rate is 91.11%-94.95%, and emergence rate is 89.77%-96.89%;5) emerge and cultivate and transplant: carry out 4 cultivations of emerging of thoughtful 6 weeks and obtain tree seedling, and at tree seedling Transplanting after first pair of true leaf is fully deployed, transplanting survival rate is 89.77%-96.89%.This seeding growing seedlings method is for being Ornamental peonies kind Paeonia delavayi, seed is directly sowed at container nursery, and operation is the most loaded down with trivial details, and the seed treatment time is long, becomes Seedling rate is more relatively low, each non-woven bag 3 seeds of sowing, wastes seed, adds production cost, and employ hot water Seed soaking, disinfecting time is long, and root culture employs greenhouse, and Dormancy breaking is also under the conditions of indoor low temperature, and the time is relatively long Some, cost is the most of a relatively high.
" science breeding " the 12nd phase in 2013 discloses " oil Paeonia suffruticosa development and high-yield cultivation technique thereof ", concrete grammar For: between the Limit of Heat to White Dew, use to broadcast sowing and sow with drilling method, line-spacing 20cm, the degree of depth is less than 3.5cm, earthing 2-3cm, Mid or late October secondary soil-covering covering film, do not carry out data explanation to emergence rate and the cycle of emerging." gardens, town and country " 2014 5th phase disclosed " oil Paeonia suffruticosa seeding and seedling raising and cultivation technique pre-test " also refer to oil seed of Paeonia suffruticosa and processes, and uses before sowing 50 DEG C of water seed soaking 3d, or with room temperature cold water seed soaking 6d, make seed coat soften and expand, it is also possible to chemical agent 200ppm gibberellins soaks Plant breaking dormancy;The method being only referred in literary composition to use, but not test and data supporting, the most to emergence rate with emerge Cycle carries out data explanation.
Summary of the invention
In order to solve above-mentioned multiple technical problem, it is an object of the invention to provide a kind of oil seeding and seedling raising side of Paeonia suffruticosa Method.The method has that the seed treatment time is short, method for treating seeds simple, it is neat to emerge, emergence rate is high, planting percent is high, nursery Transplant after low cost, seedling not by advantages such as seasonal effect.
The present invention is achieved by the following technical solutions:
A kind of oil seeding growing seedlings method of Paeonia suffruticosa, including collection and the process of oil seed of Paeonia suffruticosa, the storage of seeds, Manage after bag, transplanting on seedbed preparation, seeding and seedling raising, shifting bud, specifically comprise the following steps that
1) seed collection and process: gather mellow fruit (planting pod is the fruit of buff) in mid or late August, after gathering Depositing in shady and cool moist place, stir 1-2 time every day, in order to avoid generating heat, allowing seed physiological maturity in kind of pod, when more than 85% Fruit dry and cracked, seed is deviate from naturally, only plants by wet concentration method before the storage of seeds;
2) the storage of seeds: in storage environment is conventional booth, temperature range is 20 DEG C-28 DEG C;By pure seed before storage Sterilization, rinses well with clear water, then soaks seed 10-12 hour by normal-temperature water, spreads at cool place dry to planting crust receipts, is divided by seed Layer is laid in the wet sand after disinfecting storage, and every layer covers husky 3-5cm, and the water content of wet sand is 10%-15% weight, husky Tibetan Time is 10-15 days;
3) seedbed prepares: seedling bed uses husky bed, is cut into lime brick, and filling sand is fresh river sand;
4) seeding and seedling raising: by step 2) Sha Zanghou seed with plant growth regulator soak after, be sowed at sterilized educating In Seedling sand bed, using the mode of drilling, cover with thin straw screen or mat after broadcasting, trickle keeps seedbed to moisten, and hides with sunshade net above seedbed The moon, until seed germination;
5) bag on bud is moved: displaying for the first time when seed shows as cotyledon on husky bed, lifting moves bud, moves to the non-woven fabrics equipped with substrate In Dai, field planting, drench foot after having planted and determine root water;
6) managing after transplanting: after sprout is transplanted into container, put neat, surrounding river sand encloses and blocks, and shelters from heat or light with sunshade net, drenches Water moisturizing, after seedling grows 2-3 sheet true leaf, spray disinfectant liquid disinfectant.
It should be understood that step 1) in wet concentration method only plant and refer to, seed is totally submerged in water, not plump kind and in spite of illness The bad seed of worm floats, and breeding sinks, and removes the seed of floating.Step 3) in the husky bed that used of nursery select at nursery lot Near, physical features is smooth, build above excessively drained field, is built into lime brick, husky bedside 1.0-1.2m, length is because of chosen place Piece determine, highly (from ground height) up about 20cm.Step 4) in use the mode of drilling to refer on husky bed to ditch, Seed is uniformly sowed in ditch every 2.0cm-2.5cm, line-spacing 5cm-7cm, ditch depth 2-3cm, then covers Sha Gaiping.Step 5) in Lifting refer to sprout length to 1.5cm-2.0cm, cotyledon is not deployed, plants shell when not falling off, before 10 AM or afternoon three Play sprout after Dian, pull up sprout wet towel by bamboo let auxiliary or wet newspaper hides moisturizing, pack in time.Step 5) in fixed Plant sprout to refer to insert container bag central authorities with a thin bamboo let, hold sprout and put into cave, away from the 1.5cm-2.0cm of hole, using bamboo Sign and lean on soil with 45° angle to sprout, make sprout root tightly be combined with soil.Each container field planting 1 strain Paeonia suffruticosa sprout.
Preferably, the oil seeding growing seedlings method of Paeonia suffruticosa as above, wherein step 2) in before storage to pure seed Disinfectant used by sterilization is 1000 times of liquid of 50% carbendazol wettable powder, and disinfecting time is 30 minutes.
Preferably, the oil seeding growing seedlings method of Paeonia suffruticosa as above, wherein step 2) in before storage to wet husky sterilization Disinfectant used is 800 times of liquid of 50% carbendazol wettable powder, and disinfecting time is 30 minutes to 60 minutes.
Preferably, the oil seeding growing seedlings method of Paeonia suffruticosa as above, wherein step 3) in the husky bed that used of nursery Middle husky thick 16-18cm, seedbed spacing is 30-50cm, and the rugosity of river sand used is 0.2mm-0.5mm.
Preferably, the oil seeding growing seedlings method of Paeonia suffruticosa as above, wherein step 4) in husky hide after seed be through Cross the band root seed of root length 1.5cm-2.0cm after sand is hidden.
Preferably, the oil seeding growing seedlings method of Paeonia suffruticosa as above, wherein step 4) in plant growing used adjust Joint agent is gibberellins (GA3), concentration is 150mg/L, and seed soak time is 12 hours.
Preferably, the oil seeding growing seedlings method of Paeonia suffruticosa as above, wherein step 5) in substrate used be volume The peat of ratio=6:1:1:2, perlite, Vermiculitum and chicken manure, through 800 times of liquid disinfectants of 50% carbendazol wettable powder, substrate Water content be maintained at 30% weight to 60% weight.
Preferably, the oil seeding growing seedlings method of Paeonia suffruticosa as above, wherein step 6) in the screening of sunshade net used Light rate is 70-80%, first 10 day every day of sprinkle water 1-2 time after just transplanting, and keeps moistening, trickle 1-2 time weekly afterwards in 10 days, Do not do and do not water, water, irrigate.
Preferably, the oil seeding growing seedlings method of Paeonia suffruticosa as above, wherein step 6) in disinfectant solution used be 50% carbendazol wettable powder 1000 times liquid, sprays 1 time for every 10 days.
It is further preferred that the oil seeding growing seedlings method of Paeonia suffruticosa as above, wherein said oil seed of Paeonia suffruticosa Seed for Paeonia papaveracea PaeoniaRockii.
Compared with prior art, the oil that the present invention relates to has the advantage that with the seeding growing seedlings method of Paeonia suffruticosa and improves Property:
(1) shorten growing-seedling period, improve breeding efficiency.The fast seedling growing cycle of ornamental peonies ' Paeonia delavayi ' is 24 weeks, oil It is generally 30 weeks with traditional method for culturing seedlings growing-seedling period of Paeonia suffruticosa, and before seed sand of the present invention is hidden, disinfecting time is only 30 minutes, Seed soaking normal-temperature water, seed soaking time is less than 12 hours, and oil is 15-16 week with the growing-seedling period of Paeonia suffruticosa, and centre need not Indoor low temperature breaking dormancy.
(2) rooting rate is improved.The rooting rate of ornamental peonies ' Paeonia delavayi ' is 91.11%-94.95%, and oil of the present invention is used The rooting rate of Paeonia suffruticosa is more than 99%.
(3) emergence rate is improved.The emergence rate of ornamental peonies ' Paeonia delavayi ' is 89.77%-96.89%, and Paeonia suffruticosa ' purpura used by oil Paeonia suffruticosa ' emergence rate of conventional field seedling raising technology is only 49%, and the emergence rate of the present invention is more than 99%.
(4) planting percent is improved.Ornamental peonies ' Paeonia delavayi ' transplanting survival rate is 89.77%-96.89%, and the present invention uses In sprout, bag is transplanted, and survival rate is high, and transplanting survival rate reaches more than 99%.
(5) seedling cost is reduced.The present invention need not Soaking in hot water, it is not necessary to Low-temperature culture, upper bag after seed germination, often 1 seed of individual non-woven bag, has saved seed, has greatly reduced production cost.
(6) Phase ofsprouting is improved.Oil uses sowing in the field method with Paeonia suffruticosa, and not only emergence rate is low, and it is the most whole to emerge Together, reducing commercial seedling quality, the method for culturing seedlings that the present invention uses, emergence rate is high, emerges neat, and seedlings root is flourishing, significantly Improve Quality of Seedlings.
(7) method for culturing seedlings innovation.In the sprout that the present invention uses, bag is explored with belonging in Paeonia suffruticosa seedling-raising technique first at oil, Using the method for culturing seedlings of the present invention, seed germination rate and planting percent all reach more than 99%, and growing-seedling period is tradition nursery contracting Short about 90 days.
(8) accelerate industry and promote paces, become transplantation of seedlings not to be subject to seasonal restrictions.In sprout of the present invention, bag grows up to finished product Seedling, root Can plant at any time according to industry development demand and not be subject to seasonal restrictions.
In a word, the present invention is by the collection of oil seed of Paeonia suffruticosa and process, the storage of seeds, seedbed preparation, seeding and seedling raising, shifting Bag on bud, transplant after the measure such as management, shorten seedling raise period, improve breeding efficiency, saved seedling cost, improve out Seedling regularity and emergence rate, substantially increase seedling quality, plantation after bag survives in sprout, can not be subject to seasonal restrictions, be conducive to oil Being up to more than 99% with developing of Paeonia suffruticosa industry, rooting rate and germination rate, emerge neat, the seedlings root obtained is flourishing, Fibrous root is many, and sprout is sturdy, has wide market application foreground.
Detailed description of the invention
Following example further describe implementation process and the beneficial effect of the inventive method, and embodiment is only used for illustration Purpose, does not limits the scope of the invention, the obvious change that those of ordinary skill in the art are made according to the present invention simultaneously Within being also contained in the scope of the invention.It addition, reagent of the present invention, wherein:
GA3Chemical composition be gibberellins, be configured to high concentration mother solution, i.e. 0.5mg/mL during use.Concrete configuration method: Weigh 50 milligrams of GA3, first dissolve with a small amount of 95% ethanol, be then settled to 100 milliliters with distilled water, can use.
The chemical composition of carbendazim is 2-benzimidazole ylcarbamic acid methyl ester, is the efficiently interior absorption of a kind of broad spectrum type low toxicity Antibacterial, used in the present invention is 50% carbendazol wettable powder, is configured to high concentration mother solution during use, is 200 times Liquid.Concrete compound method: weigh 1g carbendazim, be settled to 200ml with clear water, can use.
Embodiment 1: seed soaking
The Paeonia papaveracea seed that JIUYUE in 2014 will be planted by wet concentration method only on the 30th, with the carbendazol wettable powder of 50% 1000 times of liquid disinfectants 30 minutes, rinse clean with clear water, then soak seed by normal-temperature water, and seed soaking time arranges 3 process, and respectively 12 Hour, 24 hours, 48 hours, often process 100 seeds, 3 times repeat.Pick up after process to spread to receive to kind crust at cool place and do. Seed divides the carbendazim wettability being laid on through 50% seal in the husky bed that 800 times of liquid disinfectants of agent are crossed, and every layer covers husky 4cm, sand Water content is 10%-15%, and the humidity of sand is for hold preferably agglomerating not water outlet, and the place of storage is common booth, temperature Scope is 23-25 DEG C.Stratification time is 15 days, and situation of the taking root statistical result of seed is as follows:
The impact that oil seed of Paeonia suffruticosa is taken root by the different seed soaking time of table 1
Seed soaking time/h Rooting rate/% Main root length/cm
12 99.33±0.33a 1.61±0.11a
24 99.00±0.58a 1.67±0.07a
48 99.33±0.33a 1.77±0.14a
Use the seed-soaking method of embodiment 1, lamination after 15 days the rooting rate of oil seed of Paeonia suffruticosa all reach more than 99%, and Main root length difference is the most notable.
Embodiment 2: husky Tibetan Stratification time
The Paeonia papaveracea seed that JIUYUE in 2014 will be planted by wet concentration method only on the 30th, with the carbendazol wettable powder of 50% 1000 times of liquid disinfectants 30 minutes, rinse clean with clear water, pick up and spread extremely outside kind in the cool place at after then soaking seed 12 hours by normal-temperature water Skin is received dry.Seed divides the carbendazim wettability being laid on through 50% seal in the husky bed that 800 times of liquid disinfectants of agent are crossed, and every layer covers sand 4cm, husky water content is 10%-15%, and the humidity of sand is for hold preferably agglomerating not water outlet, and the place of storage is common big Canopy, temperature range is 23-25 DEG C.Stratification time arranges 3 process, respectively 5 days, 10 days, 15 days, often processes 100 seeds, Repeat for 3 times.Situation of the taking root statistical result of seed is as follows:
The impact that oil seed of Paeonia suffruticosa is taken root by table 2 different husky Tibetan Stratification time
Husky Tibetan Stratification time/d Rooting rate/%
5 30.00±3.79a
10 87.67±2.40b
15 99.67±0.33c
Use the husky of embodiment 2 to hide lamination method, lamination after 15 days the rooting rate of oil seed of Paeonia suffruticosa all reach 99% with On, rooting rate hides 5 days and 10 days apparently higher than sand.
Embodiment 3: seeding and seedling raising
On October 13rd, 2014, the seed gibberellin of plant growth regulator (GA taken root for 15 days will be hidden through sand3) leaching Bubble processes, and processes and arranges concentration and be respectively 100ppm, 150ppm, 200ppm, process set of time be 12 hours, 24 hours, 48 Hour, it is sowed at the carbendazim wettability through 50% after process and seals the nursery sand bed crossed of 800 times of liquid disinfectants of agent (early September is at Seedling Ground, garden physical features selected around is smooth, build husky bed above excessively drained field, and husky bed lime brick is built into, husky bedside 1.0- 1.2m, length determines because of selected plot, the height about 20cm up from ground, and husky thick 16-18cm, seedbed spacing is 40cm, fills out Filling husky is fresh river sand, and rugosity is 0.3mm.In), use the mode of drilling, the seed (root length 1.5-2.0cm) of Sha Zanghou is broadcast Kind in husky bed, seed is sprinkled upon in ditch uniformly, line-spacing 6cm, ditch depth 2-3cm, then covers husky lid flat and with the covering of thin straw screen or mat, Use watering can trickle, drench every day 1-2 time and ensure that seedbed moistens, shelter from heat or light with the sunshade net of 60% above seedbed.Seed was opened February 15 Beginning to sprout, reached February 22 to sprout peak, germination rate situation is as follows:
The impact that oil is sprouted by table 3 variable concentrations HORMONE TREATMENT different time with seed of Paeonia suffruticosa
Using embodiment 3 variable concentrations to process the method for culturing seedlings of different time rapidly, oil exists with Paeonia suffruticosa Paeonia papaveracea seed Gibberellins concentration is that 150ppm soaks seed more than 12 hours, and germination rate can reach more than 90%, and wherein gibberellins concentration is 150ppm Processing 12 hours and 24 hours, the germination rate of oil seed of Paeonia suffruticosa can reach 99%.
Embodiment 4: upper bag of transplanting seedlings
Upper bag of transplanting seedlings is provided with 3 periods, period 1 be sprout length to 1.5-2.0cm, show as cotyledon and display for the first time but do not open up Open, plant shell and do not fall off;Period 2 is to launch at the beginning of a length of about the 2.5-3.0cm of sprout, cotyledon, plants shell and comes off;Period 3 is that sprout is long Go out 2-3 sheet true leaf.30 sprouts of each process, repeat for 3 times.Method particularly includes: before 10 AM or at 3 in afternoon it Rear sprout, pulls up sprout wet towel by bamboo let auxiliary or wet newspaper hides moisturizing, pack in time.Used by transplanting appearance Device is biological degradability non-woven bag, and cylindrically, height is 16cm, and at upper opening, bore is 12cm.Substrate used is V (peat): V (perlite): V (Vermiculitum): V (chicken manure)=6:1:1:2, substrate is through 800 times of liquid of 50% carbendazol wettable powder Sterilization, the water content of substrate is maintained at 30% weight to 60% weight.Move bud and before noon the container filling substrate is watered permeable, bud Sufficient water is also drenched in seedbed.Insert container bag central authorities with a thin bamboo let after moving bud, hold sprout and put into cave, from hole 1.5cm- Lean on soil with 45° angle to sprout with bamboo let at 2.0cm, make sprout root tightly be combined with soil.Each container field planting 1 strain Paeonia suffruticosa bud Seedling.After having transplanted, root water is determined in pouring in time.After sprout is transplanted into container, putting neat, surrounding river sand encloses and blocks, and with shading rate is The sunshade net of 75% shelters from heat or light, first 10 day every day of sprinkle water 1-2 time after just transplanting, and keeps moistening, 10 days trickle 1-2 the most weekly Secondary, do not do and do not water, water, irrigate, after seedling grows 2-3 sheet true leaf, spray disinfectant liquid disinfectant, sprays 1 time for every 10 days.Transplant Checking planting percent after 30 days, concrete condition statistics is as follows:
The different Different Transplanting Periods of table 4 is on the oil impact of Paeonia suffruticosa seedling planting percent
Different Transplanting Periods Planting percent
1 99.67±0.33c
2 96.33±0.88b
3 89.00±1.15a
The method using embodiment 4, moves sprout and enters non-woven bag point 3 periods, wherein Different Transplanting Periods 1 and seedling in period 2 Rate can reach more than 96%, transplants planting percent in period 1 the highest, can reach more than 99%.
Embodiment 5: seedling bed is tested
According to the operation of example 1-4, wherein the clean seed of purpura with 50% 1000 times of liquid disinfectants of carbendazol wettable powder 30 minutes, soak seed 12 hours by normal-temperature water after rinsing only with clear water, divide the carbendazim wettability being laid on through 50% to seal agent 800 times In the husky bed that liquid disinfectant is crossed, every layer covers husky 4cm, and husky water content is 10%-15%, and the humidity of sand is not for agglomerating going out to hold Preferably, the place of storage is common booth to water, and temperature range is 23-25 DEG C, will be 150ppm by concentration after earthing of seeds 15 days Gibberellin of plant growth regulator (GA3) soak 12 hours, it is sowed at the carbendazim wettability through 50% respectively and seals agent 800 times In the nursery sand bed that liquid disinfectant is crossed and soil bed, soil bed is identical with the specification of husky bed, respectively broadcasts 100, repeats for 3 times.Specifically go out the growth of cereal crop seedlings Condition statistics is as follows:
The different seedling bed impact on oil seed germination of table 5
Seedling bed Start sprout time Germination rate/%
Husky bed On February 15th, 2015 99.33
Soil bed On February 23rd, 2015 68.67
Using husky bed and the soil bed nursery respectively of embodiment 5, the germination rate of husky bed seed is 99.33%, and soil bed is only 68.67%, and husky bed seed germination will be prior to the sprouting in a week of soil bed.

Claims (10)

1. the oil seeding growing seedlings method of Paeonia suffruticosa, the method include the collection of oil seed of Paeonia suffruticosa and process, the storage of seeds, Manage after bag, transplanting on seedbed preparation, seeding and seedling raising, shifting bud, specifically comprise the following steps that
1) seed collection and process: gather mellow fruit in mid or late August, i.e. plants the fruit that pod is buff, deposits after gathering Being put in shady and cool moist place, stir 1-2 time every day, in order to avoid generating heat, allowing seed physiological maturity in kind of pod, when more than 85% Fruit is dry and cracked, and seed is deviate from naturally, only plants by wet concentration method before the storage of seeds;
2) the storage of seeds: in storage environment is conventional booth, temperature range is 20 DEG C-28 DEG C;Before storage, pure seed is sterilized, Rinse well with clear water, then soak seed 10-12 hour by normal-temperature water, spread at cool place dry, by seed place Min layers to planting crust receipts Being placed in the wet sand after disinfecting storage, every layer covers husky 3-5cm, and the water content of wet sand is 10%-15% weight, and the husky Tibetan time is 10-15 days;
3) seedbed prepares: seedling bed uses husky bed, is cut into lime brick, and filling sand is fresh river sand;
4) seeding and seedling raising: by step 2) after the seed of Sha Zanghou soaks with plant growth regulator, it is sowed at sterilized nursery husky In Chuan, using the mode of drilling, cover with thin straw screen or mat after broadcasting, trickle keeps seedbed to moisten, and shelters from heat or light with sunshade net, directly above seedbed To seed germination;
5) bag on bud is moved: displaying for the first time when seed shows as cotyledon on husky bed, lifting moves bud, moves to the non-woven bag equipped with substrate In, field planting, drench foot after having planted and determine root water;
6) managing after transplanting: after sprout is transplanted into container, put neat, surrounding river sand encloses and blocks, and shelters from heat or light with sunshade net, and trickle is protected Wet, after seedling grows 2-3 sheet true leaf, spray disinfectant liquid disinfectant.
The oil seeding growing seedlings method of Paeonia suffruticosa the most according to claim 1, it is characterised in that: step 2) in before storage to pure Clean disinfectant used by seed disinfection is 1000 times of liquid of 50% carbendazol wettable powder, and disinfecting time is 30 minutes.
The oil seeding growing seedlings method of Paeonia suffruticosa the most according to claim 1, it is characterised in that: step 2) in before storage to wet Disinfectant used by husky sterilization is 800 times of liquid of 50% carbendazol wettable powder, and disinfecting time is 30 minutes to 60 minutes.
The oil seeding growing seedlings method of Paeonia suffruticosa the most according to claim 1, it is characterised in that: in step 3), nursery is used Husky bed in husky thick 16-18cm, seedbed spacing is 30-50cm, and the rugosity of river sand used is 0.2mm-0.5mm.
The oil seeding growing seedlings method of Paeonia suffruticosa the most according to claim 1, wherein in step 4) husky hide after seed be through Cross the band root seed of root length 1.5cm-2.0cm after sand is hidden.
The oil seeding growing seedlings method of Paeonia suffruticosa the most according to claim 1, it is characterised in that: plant used in step 4) Growth regulator is gibberellins GA3, concentration is 150mg/L, and seed soak time is 12 hours.
The oil seeding growing seedlings method of Paeonia suffruticosa the most according to claim 1, it is characterised in that: substrate used in step 5) For peat, perlite, Vermiculitum and the chicken manure of volume ratio=6:1:1:2, through 800 times of liquid disinfectants of 50% carbendazol wettable powder, The water content of substrate is maintained at 30% weight to 60% weight.
The oil seeding growing seedlings method of Paeonia suffruticosa the most according to claim 1, it is characterised in that: sunshade used in step 6) The shading rate of net is 70-80%, first 10 day every day of sprinkle water 1-2 time after just transplanting, and keeps moistening, 10 days trickles the most weekly 1-2 time, do not do and do not water, water, irrigate.
The oil seeding growing seedlings method of Paeonia suffruticosa the most according to claim 1, it is characterised in that: sterilization used in step 6) Liquid is 1000 times of liquid of 50% carbendazol wettable powder, within every 10 days, sprays 1 time.
10. according to the claim 1-9 any one oil seeding growing seedlings method of Paeonia suffruticosa, it is characterised in that: Paeonia suffruticosa used by described oil Seed is the seed of Paeonia papaveracea PaeoniaRockii.
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