CN109005735A - A kind of trollflower container seedling culture method - Google Patents

A kind of trollflower container seedling culture method Download PDF

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Publication number
CN109005735A
CN109005735A CN201810827787.7A CN201810827787A CN109005735A CN 109005735 A CN109005735 A CN 109005735A CN 201810827787 A CN201810827787 A CN 201810827787A CN 109005735 A CN109005735 A CN 109005735A
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CN
China
Prior art keywords
rush
seed
container
seedling
culture method
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Pending
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CN201810827787.7A
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Chinese (zh)
Inventor
陈虞超
郭生虎
宋玉霞
关雅静
朱永兴
李明
巩檑
张丽
甘晓燕
聂峰杰
刘璇
安钰
刘华
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Agricultural Biotechnology Research Center Of Ningxia Academy Of Agriculture And Forestry Sciences (ningxia Key Laboratory Of Agricultural Biotechnology)
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Agricultural Biotechnology Research Center Of Ningxia Academy Of Agriculture And Forestry Sciences (ningxia Key Laboratory Of Agricultural Biotechnology)
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Priority to CN201810827787.7A priority Critical patent/CN109005735A/en
Publication of CN109005735A publication Critical patent/CN109005735A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

Abstract

The present invention provides a kind of container seedling culture methods of trollflower, are related to field of plant growing technology.After the method for the invention is the following steps are included: lily feet flower seed is impregnated in water, then liquid is sprouted with rush and is handled, obtain rush and sprout seed;Seedling medium is filled up in container for plant growth, and implantation hole is set in seedling medium;The rush is sprouted seed to be seeded in the implantation hole, the seedling medium of 0.1~0.3cm is after planting covered in the implantation hole, is smoothed out, is poured primary permeable;After the rush sprouts seed sprouting, a water was poured in shading every 2~3 days;After seedling grows the 1st true leaf completely, sprayed every 10 days foliar fertilizer 1 time;The 5th~6 true leaf is grown to seedling, and after plant height is more than 15cm, be out of the garden transplanting.The present invention carries out container nursery to lily feet flower seed, trollflower seed germination rate can be made to reach 98% or more, planting percent reaches 96% or more, and transplanting survival rate reaches 98% or more.

Description

A kind of trollflower container seedling culture method
Technical field
The invention belongs to field of plant growing technology, and in particular to a kind of trollflower container seedling culture method.
Background technique
Trollflower (Trollius chinensis Bunge. scientific name China trollflower) is Ranunculaceae (Ranunculaceae) Trollius (TrolliusL.) perennial herb plant, is used as medicine with its dry flower, is had and is controlled mouth Sore, larynx be swollen, superficial heat pingival atrophy, ear pain, mesh pain, improving eyesight, solution haze miasma, furunculosis, big malicious all wind and other effects, treat respiratory tract infection, Pharyngitis, tonsillitis, bronchitis etc. tool have a better effect, and are one of clinical most common Chinese medicines.Produce lily feet Flower has very high economic benefit.
Trollflower market demand cumulative year after year, but its raw material relies primarily on excavation wild resource, and yield increase is extremely It is limited, cause trollflower production to need contradiction epitonos, market price Continued.Trollflower artificial growth remarkable in economical benefits, Show great potential.Trollflower relies primarily on seed and is bred, but there are physiological dormancy phenomenon, natural items for its seed Seed germination rate and planting percent are lower under part.It would therefore be highly desirable to which artificial technology's means is taken to carry out nursery, lily feet flower seed is improved Emergence rate and planting percent, promote its plantation and exploitation.
Currently, trollflower artificial breeding mostly uses crop field or greenhouse to carry out level bed nursery, and that there are breeding efficiencies is low, is out of the garden The problems such as operation difficulty is big, transplanting survival rate is low.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of method of trollflower container nursery, the method for the invention It can effectively solve the problem that trollflower seed germination rate and the problem that planting percent is low, transplanting survival rate is low, be suitable for trollflower scale Seedling breeding during artificial growth.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of container seedling culture methods of trollflower, comprising the following steps:
1) after impregnating lily feet flower seed in water, then liquid is sprouted with rush and is handled, obtain rush and sprout seed;It is to include that the rush, which sprouts liquid, GA3And NaHCO3Aqueous solution;
2) seedling medium is filled up in container for plant growth, and implantation hole is set in the seedling medium;The seedling medium packet Include peat and vermiculite;
3) rush for obtaining step 1) is sprouted seed and is seeded in the step 2) implantation hole, after planting in the plantation The seedling medium that 0.1~0.3cm thickness is covered on hole, smoothes out, sprinkles profoundly water;
4) after the rush sprouts seed sprouting, a water was poured in shading every 2~3 days;
5) it after seedling grows the 1st true leaf completely, was sprayed every 10 days foliar fertilizer 1 time;
6) it grows the 5th after seedling to obtain after 6 true leaves and plant height are more than 15cm, be out of the garden transplanting;
There is no temporal sequences to limit for step 1) and step 2).
Preferably, the time impregnated in the step 1) water is 12~36h.
Preferably, the step 1) rush sprouts GA in liquid3Concentration be 5~15g/L, NaHCO3Concentration be 25~75g/L.
Preferably, the step 1) rush sprouts liquid processing to sprout 48~120h of immersion in liquid in the rush.
Preferably, the step 2) container for plant growth includes hole tray.
Preferably, the volume ratio of the step 2) peat and vermiculite is (1~4): 1.
Preferably, the step 3) sowing is that 3~5 rush of sowing sprout seed in each implantation hole.
Preferably, described after planting in step 3), before sprouting seed sprouting to rush described in step 4), every watering 1 in 3~5 days It is secondary.
Preferably, the shading rate of the step 4) shading is 50~75%.
The present invention provides a kind of methods of trollflower container nursery, carry out suspend mode to lily feet flower seed and abolish processing, promote It is sprouted into lily feet flower seed, improves germination rate, suspend mode is abolished after processing 4 days, and trollflower seed germination rate reaches 98% or more; After culture 75 days, planting percent reaches 96% or more;Culture 90~105 days after, can be transplanted, transplanting survival rate reach 98% with On.
Detailed description of the invention
Fig. 1 is that 1 trollflower of the embodiment of the present invention sows the seedling growth shape after 70d.
Specific embodiment
The present invention provides a kind of container seedling culture methods of trollflower, comprising the following steps:
1) after impregnating lily feet flower seed in water, then liquid is sprouted with rush and is handled, obtain rush and sprout seed;It is to include that the rush, which sprouts liquid, GA3And NaHCO3Aqueous solution;
2) seedling medium is filled up in container for plant growth, and implantation hole is set in the seedling medium;The seedling medium packet Include peat and vermiculite;
3) rush for obtaining step 1) is sprouted seed and is seeded in the step 2) implantation hole, after planting in the plantation The seedling medium that 0.1~0.3cm thickness is covered on hole, smoothes out, sprinkles profoundly water;
4) after the rush sprouts seed sprouting, a water was poured in shading every 2~3 days;
5) it after seedling grows the 1st true leaf completely, was sprayed every 10 days foliar fertilizer 1 time;
6) after seedling grows the 5th or 6 true leaf and plant height is more than 15cm, be out of the garden transplanting;
There is no temporal sequences to limit for step 1) and step 2).
In trollflower container seedling culture method of the invention, after lily feet flower seed is impregnated in water, then with promote sprout at liquid Reason, obtains rush and sprouts seed;It includes GA that the rush, which sprouts liquid to be,3And NaHCO3Aqueous solution.Lily feet flower seed of the present invention preferably at Ripe full lily feet flower seed, more preferably diameter be 0.5mm sieve pore screen for seeds after the seed that leaves.The present invention is to described There is no particular determinations in the source of seed.Immersion of the present invention is impregnated preferably by tap water, and the time of the immersion is preferred For 12~36h, more preferably 16~30h, most preferably 20~for 24 hours.After the present invention pulls the seed impregnated with tap water out, use Rush sprouts liquid processing.It includes GA that rush of the present invention, which sprouts liquid to be,3And NaHCO3Aqueous solution, the concentration of the GA3 is preferably 5~ 15g/L, more preferably 7~12g/L, most preferably 9~10g/L.Rush of the present invention sprouts NaHCO in liquid3Concentration be preferably 25~75g/L, more preferably 35~60g/L, most preferably 45~50g/L.The processing time of the present invention for promoting to sprout liquid is preferred For 48~120h, more preferably 72~100h, most preferably 96h.After the present invention pulls the seed through promoting to sprout liquid processing out, preferably It further include tap water flushing and dry process, the number that the tap water rinses is preferably 2~3 times.The present invention is to the punching There is no particular determinations for the water consumption washed, and are rinsed using the conventional method of this field.Temperature of the present invention to the drying There is no particular determinations, utilize air drying.Rush of the present invention is sprouted in liquid, and bicarbonate soda can soften kind of a skin, and alkali Property is low, relatively small to Seed Germination;GA3Seed dormancy is abolished, promotes to sprout.GA of the present invention3And NaHCO3It is dense Degree can fully ensure that seed germination rate, and also having reduces cost, reduces the influence to seedling, such as the gibberellin and carbonic acid of high concentration Hydrogen sodium, the growth effect of seedling is larger after sprouting, sprouting to seed;Gibberellin excessive concentration is easy to cause the generation of lopsided seedling. Method for culturing seedlings of the present invention preferably carries out in the conventional agriculture or horticultural production facility with certain heat and moisture preserving function, more Preferably heliogreenhouse or arched shed, temperature and humidity needed for guaranteeing the sprouting of trollflower seed, growth of seedling.
The present invention fills up seedling medium in container for plant growth, and implantation hole is arranged in the seedling medium;The nursery base Matter includes peat and vermiculite.Container for plant growth of the present invention preferably includes hole tray, source and material of the present invention to the hole tray There is no particular determination, preferably 50 hole hole trays of PS material, the thickness of the hole tray be preferably 1mm, hole tray size be 540* 285mm, hole tray hole depth 90mm.The volume ratio of peat and vermiculite of the present invention is (1~4): 1, more preferably (1.5~3): 1, Most preferably 2:1.In the present invention, peat can guarantee that matrix contains sufficient nutritional ingredient, and vermiculite can guarantee that matrix has There is sufficient breathable and permeable function.Implantation hole is arranged in the present invention in the seedling medium, and the implantation hole is preferably placed at hole tray The central location in hole.The diameter of implantation hole of the present invention is preferably 0.5~1cm.The depth of implantation hole of the present invention is preferred For 0.5~1cm.
It obtains after promoting to sprout seed, the rush is sprouted seed and is seeded in the step 2) implantation hole by the present invention, after planting exists The seedling medium that 0.1~0.3cm thickness is covered in the implantation hole, smoothes out, sprinkles profoundly water.Preferably each kind of sowing of the present invention It plants 3~5 rush of sowing in hole and sprouts seed.There is no particular determinations for mode of the present invention to the watering, preferably pass through micro- spray Mode.The present invention preferably watered 1 time every 3~5 days after the completion of the sowing, and the time watered every time is preferably 30~ 60min。
For the present invention after the rush sprouts seed sprouting, a water was poured in shading every 2~3 days.Emergence of the present invention is preferred Refer to has the lily feet flower seed in 5~10 implantation holes to sprout and starts to grow from matrix in hole tray.Shading of the present invention is excellent Choosing is shaded using shading net, and the shading rate of the shading net is 50~75%.Shading rate of the present invention can both guarantee Trollflower is not burnt by strong light, and required sunlight can be grown with guarantee fund's lotus flower.The present invention is after the emergence, every 2~3 It pours a water, and the time watered every time is preferably 10~20min.
The present invention sprayed foliar fertilizer 1 time after seedling grows the 1st true leaf completely every 10 days.It is of the present invention to grow the 1st Preferably refer to that 50% or more trollflower seedling starts the 2nd true leaf occur after piece true leaf.Kind of the present invention to the foliar fertilizer And source, there is no particular determination, preferably leaf power (production of Guangzhou Piao Lv afforestation Co., Ltd), the foliar fertilizer is applied The diluted concentration of fertilizer is preferably 500~1000 times, more preferably 800~1000 times, most preferably 1000 times.Spray of the present invention It applies the foliar fertilizer time and water spray interval carries out for 2~3 days, after spraying foliar fertilizer 2 times, remove sunshade net.
After the present invention waits for that seedling grows the 5th or 6 true leaf, and plant height is more than 15cm, be out of the garden transplanting.It is of the present invention to grow 5 or 6 true leaves preferably refer to that 80% or more trollflower seedling starts the 6th true leaf occur.Plant height of the present invention is more than 15cm Preferably length of the trollflower seedling topmost true leaf of 90% or more finger apart from base portion is greater than 15cm afterwards.
Trollflower container seedling culture method provided by the invention is described in detail below with reference to embodiment, but cannot They are interpreted as limiting the scope of the present invention.
Embodiment 1
5~August in 2016 is being located at Guyuan branch of Ningxia Academy of Agri-Forestry Sciences of the village Lin Gou of the Ningxia Longde County township Guan Zhuan experiment base Ground carries out, comprising the following steps:
(1) in Experimental Base, nursery arched shed, arched shed area 100m were built May 92
(2) May 10~11 days are sufficiently mixed preparation seedling medium with 3000L peat, 1500L vermiculite, matrix are filled into In hole tray, total hole tray 400.
(3) hole tray is placed in arched shed, the stromal surface in hole tray hole center lays the kind of diameter 0.5cm, depth 0.5cm Plant hole.
(4) the lily feet flower seed of mature and plump is first impregnated 12h with tap water by May 12, seed is pulled out, then use 10g/ L GA3With 50g/L NaHCO3Mixed solution soaking 48h, then seed is pulled out, with tap water rinse 2 times, air drying.5 Lily feet flower seed is sowed in implantation hole by the moon on the 16th, 3, every hole seed;Matrix 0.2cm is after planting covered in cavities, is smoothed out, is adopted With micro- spray, sprays permeable 1 time, then sprayed water 1 time every 5 days, every time spray 30 minutes;
(5) May 28 days have the emergence of 7 cave trollflowers in every hole tray, and the shading net using shading rate 50% shades, It using micro- spray, sprayed water 1 time every 3 days, every time spray 10 minutes;
(6) June 12 days, lily feet flower seedling grow the 1st true leaf completely, and foliar fertilizer (trade name: Ye Li) 1 was sprayed every 10 days Secondary, fertilising diluted concentration is 500 times, sprays the foliar fertilizer time and water spray interval carries out for 3 days, after spraying foliar fertilizer 2 times, remove screening Screened postive;
(7) August 15 days, lily feet flower seedling grow the 6th true leaf, and the plant height of 95% plant has been more than 15cm, starts the shifting that is out of the garden It plants.
Test statistics analysis: after lily feet flower seed sows 70d, as shown in Figure 1, trollflower seed germination rate is 98.3%, Planting percent is 96.3%, transplanting survival rate 98.6%.
Embodiment 2
4~July in 2017 is being located at the progress of Institute of Horticultural, Ningxia Academy of Agriculture and Forestry Sciences's Experimental Base, comprising the following steps:
(1) the complete heliogreenhouse of selection facility.
(2) April 10~11 days are sufficiently mixed preparation seedling medium with 9000L peat, 4500L vermiculite, matrix are filled into In hole tray, total hole tray 1200.
(3) hole tray is placed in heliogreenhouse, the stromal surface in hole tray hole center lays diameter 0.5cm, depth 1cm Implantation hole.
(4) April 12 first impregnated the lily feet flower seed of mature and plump for 24 hours with tap water, seed was pulled out, then use 5g/L GA3And 25g/LNaHCO3Mixed solution soaking 96h, then seed is pulled out, with tap water rinse 3 times, air drying.April Lily feet flower seed was sowed in implantation hole in 20,5, every hole seed;Matrix 0.3cm is after planting covered in cavities, is smoothed out, and is used Micro- spray is sprayed permeable 1 time, was then sprayed water 1 time every 3 days, every time spray 60 minutes.
(5) April 28 days have the emergence of 7 cave trollflowers in every hole tray, and the shading net using shading rate 75% shades, It using micro- spray, sprayed water 1 time every 2 days, every time spray 20 minutes.
(6) May 11 days, lily feet flower seedling grow the 1st true leaf completely, and foliar fertilizer (trade name: Ye Li) 1 was sprayed every 10 days Secondary, fertilising diluted concentration is 1000 times, sprays the foliar fertilizer time and water spray interval carries out for 3 days, after spraying foliar fertilizer 2 times, remove Sunshade net.
(7) July 18 days, lily feet flower seedling grow the 6th true leaf, and the plant height of 95% plant has been more than 15cm, starts the shifting that is out of the garden It plants.
Test statistics analysis: trollflower seed germination rate is 99.7%, planting percent 97.8%, and transplanting survival rate is 99.6%.
The present invention provides a kind of container seedling culture methods of trollflower, carry out container nursery to lily feet flower seed, can make gold Lotus flower seed germination rate reaches 98% or more, and planting percent reaches 96% or more, and transplanting survival rate reaches 98% or more.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered It is considered as protection scope of the present invention.

Claims (9)

1. a kind of container seedling culture method of trollflower, comprising the following steps:
1) after impregnating lily feet flower seed in water, then liquid is sprouted with rush and is handled, obtain rush and sprout seed;It includes GA that the rush, which sprouts liquid to be,3With NaHCO3Aqueous solution;
2) seedling medium is filled up in container for plant growth, and implantation hole is set in the seedling medium;The seedling medium includes mud Charcoal and vermiculite;
3) rush for obtaining step 1) is sprouted seed and is seeded in the step 2) implantation hole, after planting in the implantation hole The seedling medium of 0.1~0.3cm thickness is covered, smoothes out, sprinkles profoundly water;
4) after the rush sprouts seed sprouting, a water was poured in shading every 2~3 days;
5) it after seedling grows the 1st true leaf completely, was sprayed every 10 days foliar fertilizer 1 time;
6) after seedling grows the 5th or 6 true leaf and plant height is more than 15cm, be out of the garden transplanting;
There is no temporal sequences to limit for step 1) and step 2).
2. container seedling culture method according to claim 1, which is characterized in that the time impregnated in the step 1) water is 12 ~36h.
3. container seedling culture method according to claim 1, which is characterized in that the step 1) rush sprouts GA in liquid3Concentration be 5~15g/L, NaHCO3Concentration be 25~75g/L.
4. container seedling culture method according to claim 1, which is characterized in that the step 1) rush sprouts liquid processing for described Rush sprouts 48~120h of immersion in liquid.
5. container seedling culture method according to claim 1, which is characterized in that the step 2) container for plant growth includes hole tray.
6. container seedling culture method according to claim 1, which is characterized in that the volume ratio of step 2) peat and vermiculite For (1~4): 1.
7. container seedling culture method according to claim 1, which is characterized in that the step 3) sowing is in each implantation hole 3~5 rush of sowing sprout seed.
8. container seedling culture method according to claim 1, which is characterized in that it is described after planting in step 3), arrive step 4) Before the rush sprouts seed sprouting, watered 1 time every 3~5 days.
9. container seedling culture method according to claim 1, which is characterized in that the shading rate of the step 4) shading be 50~ 75%.
CN201810827787.7A 2018-07-25 2018-07-25 A kind of trollflower container seedling culture method Pending CN109005735A (en)

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Application publication date: 20181218