CN104737670A - Seedling growing method for oil expression peony seeds - Google Patents
Seedling growing method for oil expression peony seeds Download PDFInfo
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Abstract
The invention belongs to the technical field of plant seedling culture, and discloses a seedling growing method for oil expression peony seeds. The method comprises measures of collection and processing of the oil expression peony seeds, seed storage, seedbed preparation, sowing and seedling raising, management after transplantation, and the like. Compared with the prior art, the method has the advantages that the seed processing time is short, the seed processing method is simple, the emergence of seedlings is tidy, the emergence rate is high, the seedling survival rate is high, the seedling raising cost is low, no season influence of transplantation after seedling survival exists and the like.
Description
Technical field
The invention belongs to botanical seedling culturing technical field, relate to a kind of seedling-cultivating method, more particularly, the present invention relates to for oil Paeonia suffruticosa seed seedling-cultivating method.
Background technology
In recent years along with the quick raising of economy and living standards of the people, the demand of China to edible oil raises year by year, and domestic edible oil supply is obviously not enough, according to relevant statistics, 60% of China's edible oil total amount depends on import, has exceeded international edible oil safe early warning line.At present, the main oil crop soybean of China, depends on import substantially.Meanwhile, the oil crop of China are mainly herbaceous plant, and land resources is limited, and the growth space of cultivated area and output is very little.In this context, the distinctive new good quality and high output oil crop of Devoting Major Efforts To Developing China are extremely urgent.Oil is the exclusive initial species plant of China with tree peony (Paeonia suffruticosa Andr.for oil), is the general designation of the kind of producing seed oil yield high (being more than or equal to 22%) in Paeoniaceae Paeonia sect. Moutan plant.The oil content of oil peony seeds can reach 24.12%-37.83%, the content of unsaturated fatty acid can reach 92.0%, the alpha-linolenic acid content being wherein called as " plant docosapentaenoic acid " is 41.86%, higher than camellia oil, walnut oil, olive oil, sunflower oil, peanut oil and sesame wet goods edible oil, it is more than 140 times of olive oil.In addition, peony seed oil, also containing the nutriment such as distinctive Paeonol, tree peony saponin, tree peony polysaccharide, fucosterol, squalene, has the effect such as angiocardiopathy preventing, reducing blood lipid, hypotensive, anti-inflammation and sterilization, activating cell, anti-ageing, cancer-resisting.On March 22nd, 2011, Ministry of Health of the People's Republic of China's No. 9 publication approval peony seed oil, as new resource food, indicates the high-grade edible vegetable oil that peony seed oil becomes " legal ", enters edible oil ranks.No matter oil as the woody oil crop of the distinctive ecology of China with tree peony, be from itself nutritive value or stand in the strategic concerns of the safe overall situation of national grain and oil, and it all has great Development volue and market prospects.At present, it is numerous that researcher generally adopts the method for seeding and seedling raising to carry out nursery, resource conservation and breeding expansion to oil with tree peony, therefore the Research on Oil new technology of tree peony in seeding and seedling raising and seed germination, has realistic meaning for the oil further investigation of tree peony and industrialization development.
The oil proliferation of tree peony, up to the present has no particular study report.In the cultivation course of oil with tree peony, modal propagation method is sowed exactly, but because oil is thicker harder by tree peony seed coat wax, sprouting is taken root very difficult, and up to the present prior art also proposes simple and effective processing method not easily not sprouting the problem of taking root for oil Paeonia suffruticosa seed.Meanwhile, oil Paeonia suffruticosa seed also also exists epicotyl or plumule dormancy, and seed germination is irregular, and some seed does not germinate in several years and is in resting state always.Oil is subject to country and local attention with tree peony industry development and supports energetically in recent years, the expansion that its research is just like a raging fire with industry development.However, the oil of China is still in the starting stage by tree peony industry, and the research of the aspects such as seedling growing process is still the problem needing solution badly.Current oil mainly takes sowing in the field seedling raising manners with tree peony; emergence rate, regularity and go out garden transplant all be affected; cause that production efficiency is low, the loss of labour and the means of production; this oil supply breed breeding of tree peony, the research of germ plasm resource and protection work, industrialization development etc. bring very large difficulty, and therefore in the urgent need to address is at present the method effectively broken the dormancy of oil Paeonia suffruticosa seed and improve breeding breeding efficiency, nursery regularity and planting percent.
In addition, oil belongs to meat root system with the root system of tree peony, and easily sustain damage, root system comes to harm and is directly connected to transplanting survival rate.In order to effectively improve the transplanting survival rate of oil with tree peony seedling, drop to minimum with regard to needing to adopt certain method that seedlings root is transplanted injured probability.Although the mode of traditional sowing in the field can avoid oil to cause root system injured with tree peony seedling because of transplanting, but traditional type of seeding is difficult to form standardization, standardization, intensive manufacture, also, emerge irregular, the problem such as emergence rate low long along with seedling-growing time simultaneously, is unfavorable for the industrialized development of oil with tree peony.
In recent years, owing to adopting sowing in the field technology more, oil with the transplanting work of tree peony seedling be all generally Second Year after planting nine, the October carries out, one side is easy to be damaged root system, planting of transplanted seedling is also easily subject to the restriction in season on the other hand, is unfavorable for the demand of industrialized development.
Find according to retrieval, " Shaanxi agricultural science " the 6th phase in 2011 discloses the research of Paeonia papaveracea (purple butterfly windward) seeding growing seedlings method, adopt the method that fresh seeds is live, during sowing, by thin for soil deep tillage rake, open the dark ditch of 10cm with hoe, seed is spread on bottom of trench, rake with rake, the cycle of emerging is 30 weeks, and emergence rate is 49%.The sowing in the field method that this kind of method adopts, the cycle of emerging is long, and emergence rate is low.
CN201010569561.5 (name is called " fast seedling-cultivating method of a kind of Paeonia suffruticosa seed container for plant growth and this container of employing ") discloses a kind of Paeonia suffruticosa seed container for plant growth and adopts the fast seedling-cultivating method of this container, comprises the following steps: 1) test material: Paeonia delavayi seed; 2) container for plant growth: described container for plant growth comprises sidepiece and bottom, described sidepiece and/or bottom adopt biological degradability non-woven fabrics to obtain, cylindrically, be highly 7cm to 12cm, the diameter of upper opening is 4cm to 8cm, and the ratio between the diameter of described upper opening and the diameter of bottom is 1: 0.8 to 1.2, the density of non-woven fabrics is 10g/m
2to 40g/m
2, the water binding capacity of non-woven fabrics is 140g/m
2to 200g/m
2; 3) processing method of Paeonia suffruticosa seed: first adopt the potassium permanganate of 0.1 % by weight to 0.2 % by weight to soak seed 2 little of 3 hours, rinse with clear water, employing temperature is that the hot-water soak seed of 55 DEG C to 80 DEG C carried out sterilizing to 15 minutes to seed in 10 minutes, then adopt normal-temperature water Paeonia suffruticosa seed to be carried out to the seed soaking of 3 days to 5 days, finally adopt the root-inducing powder aqueous solution of 40mg/ml to 60mg/ml to soak seed 12 little of 24 hours.4) seeding growing seedlings method: sterilizing and/or seed soaking are carried out to Paeonia suffruticosa seed, in the non-woven container that matrix is housed, sow Paeonia suffruticosa seed carry out culture of rootage in greenhouse, sow 3 for every bag, matrix is the vermiculite of volume ratio 2.5-3.5:1 and the mixture of the peat composed of rotten mosses, the water content of matrix remains on 30% weight to 80% weight, sowing depth is 2.0-2.5Cm, greenhouse temperature is 15-25 DEG C, incubation time is 8-12 week, 6-12 week breaking dormancy is processed under the condition of low temperature 2-6 DEG C, rooting rate is 91.11%-94.95%, emergence rate is 89.77%-96.89%, 5) emerge and cultivate and transplant: carry out 4 thoughtful 6 weeks emerge to cultivate and obtain tree seedling, and transplant after tree seedling first pair of true leaf launches completely, transplanting survival rate is 89.77%-96.89%.This seeding growing seedlings method is for being ornamental peonies kind Paeonia delavayi, and seed is directly sowed at container nursery, and operation is relatively loaded down with trivial details, the seed treatment time is long, and planting percent is relatively lower, and each non-woven bag sows 3 seeds, waste seed, add production cost, and employ Soaking in hot water, disinfecting time is long, and culture of rootage employs greenhouse, Dormancy breaking is also under indoor low temperature condition, and the time is relatively longer, and cost is also relatively high.
" science breeding " the 12nd phase in 2013 discloses " oil tree peony development and high-yield cultivation technique thereof ", concrete grammar is: between the Limit of Heat to White Dew, employing is broadcasted sowing and is sowed with drilling method, line-spacing 20cm, the degree of depth is no more than 3.5cm, earthing 2-3cm, mid or late October secondary soil-covering covering film, data explanation is not carried out to emergence rate and the cycle of emerging." gardens, town and country " the 5th phase in 2014, disclosed " oil tree peony seeding and seedling raising and culture technique pre-test " also refer to the process of oil Paeonia suffruticosa seed, with 50 DEG C of water seed soaking 3d before sowing, or with normal temperature cold water seed soaking 6d, make the softening expansion of seed coat, also useful chemical medicament 200ppm gibberellin seed soaking breaking dormancy; Only refer to operable method in literary composition, but do not test and data supporting, also data explanation is not carried out to emergence rate and the cycle of emerging.
Summary of the invention
In order to solve above-mentioned multiple technical problem, the object of the present invention is to provide a kind of oil seeding growing seedlings method of tree peony.The method have seed treatment time short, method for treating seeds simple, emerge neat, emergence rate is high, planting percent is high, seedling cost is low, transplant by advantages such as seasonal effect after seedling.
The present invention is achieved by the following technical solutions:
The oil seeding growing seedlings method of tree peony, comprising the collection of oil Paeonia suffruticosa seed with process, seed storage, seedbed prepare, seeding and seedling raising, move bag on bud, transplant and manage afterwards, concrete steps are as follows:
1) seed collection and process: to gather mellow fruit (planting the fruit that pod is buff) in mid or late August, shady and cool moist place is deposited in after gathering, every day is stirred 1-2 time, in order to avoid heating, allow seed physiological ripening in kind of pod, when the fruit of more than 85% is dry and cracked, seed is deviate from naturally, only plants before seed storage by wet concentration method;
2) seed storage: storage environment is in conventional booth, temperature range is 20 DEG C-28 DEG C; Before storage, pure seed is sterilized, rinse well with clear water, then with normal-temperature water seed soaking 10-12 hour, spreading shady and cool place receives dry to planting crust, seed place Min layers is placed in the wet sand after disinfecting and preserves, every layer covers husky 3-5cm, and wet husky water content is 10%-15% weight, and the husky Tibetan time is 10-15 days;
3) seedbed prepares: seedling bed adopts husky bed, is cut into lime brick, and filling sand is fresh river sand;
4) seeding and seedling raising: by step 2) after the seed of Sha Zanghou soaks with plant growth regulator, be sowed in the husky bed of sterile nursery, adopt the mode of drilling, broadcast rear thin straw screen or mat to cover, trickle keeps seedbed moistening, shelters from heat or light, until seed germination above seedbed with sunshade net;
5) bag on bud is moved: display for the first time when seed shows as cotyledon on husky bed, lifting moves bud, moves to and is equipped with in the non-woven bag of matrix, field planting, the sufficient normal root water of rear pouring of having planted;
6) management after transplanting: after bud transplantation of seedlings enters container, put neat, surrounding river sand encloses and blocks, and shelters from heat or light with sunshade net, trickle moisturizing, grows after 2-3 sheet true leaf, spray disinfectant liquid disinfectant until seedling.
It should be noted that: step 1) in wet concentration method only plant and refer to, be immersed in completely in water by seed, the bad seed of not plump kind and in spite of illness worm floats, and breeding sinks, and removes the seed of floating.Step 3) in nursery adopt husky bed is selected near nursery lot, physical features is smooth, build above excessively drained field, be built into lime brick, husky bedside 1.0-1.2m, length because determining chosen place piece, highly (from ground height) up about 20cm.Step 4) in adopt the mode of drilling to refer to ditch on husky bed, be evenly sowed in ditch every 2.0cm-2.5cm by seed, line-spacing 5cm-7cm, ditch depth 2-3cm, then covers Sha Gaiping.Step 5) in lifting refer to that bud seedling grows to 1.5cm-2.0cm, cotyledon does not launch, and plants shell when not coming off, plays bud seedling before 10 AM or at 3 in afternoon after, pulls up bud seedling wet towel or wet newspaper covering moisturizing, pack in time with bamboo let is auxiliary.Step 5) in field planting bud seedling refer to and insert container bag central authorities with a thin bamboo let, hold bud seedling and put into cave, from 1.5cm-2.0cm place, hole bamboo let with 45° angle to bud seedling by native, bud shoot root portion is tightly combined with soil.Each container field planting 1 strain tree peony bud seedling.
Preferably, the oil as above seeding growing seedlings method of tree peony, wherein step 2) in be 50% carbendazol wettable powder, 1000 times of liquid to pure seed disinfectant used of sterilizing before storage, disinfecting time is 30 minutes.
Preferably, the oil as above seeding growing seedlings method of tree peony, wherein step 2) in be 50% carbendazol wettable powder, 800 times of liquid to wet sand sterilization disinfectant used before storage, disinfecting time is 30 minutes to 60 minutes.
Preferably, the oil as above seeding growing seedlings method of tree peony, wherein step 3) in husky thick 16-18cm in the husky bed that adopts of nursery, seedbed spacing is 30-50cm, and the rugosity of river sand used is 0.2mm-0.5mm.
Preferably, the oil as above seeding growing seedlings method of tree peony, wherein step 4) in husky hide after seed be through the band root seed of the long 1.5cm-2.0cm of root after husky Tibetan.
Preferably, the oil as above seeding growing seedlings method of tree peony, wherein step 4) in plant growth regulator used be gibberellin (GA
3), concentration is 150mg/L, and seed soak time is 12 hours.
Preferably, the oil as above seeding growing seedlings method of tree peony, wherein step 5) in matrix used be the peat of volume ratio=6:1:1:2, perlite, vermiculite and chicken manure, through 50% carbendazol wettable powder, 800 times of liquid disinfectants, the water content of matrix remains on 30% weight to 60% weight.
Preferably, the oil as above seeding growing seedlings method of tree peony, wherein step 6) in the shading rate of sunshade net used be 70-80%, sprinkle water 1-2 time first 10 day every day after firm transplanting, keeps moistening, trickle 1-2 time weekly after 10 days, do not do and do not water, water, irrigate.
Preferably, the oil as above seeding growing seedlings method of tree peony, wherein step 6) in thimerosal used be 50% carbendazol wettable powder, 1000 times of liquid, within every 10 days, spray 1 time.
Further preferably, the oil as above seeding growing seedlings method of tree peony, wherein said oil Paeonia suffruticosa seed is the seed of Paeonia papaveracea PaeoniaRockii.
Compared with prior art, the oil that the present invention relates to seeding growing seedlings method tool of tree peony has the following advantages and progressive:
(1) shorten growing-seedling period, improve breeding efficiency.The fast seedling growing cycle of ornamental peonies ' Paeonia delavayi ' is 24 weeks, oil is generally 30 weeks with traditional seedling-cultivating method growing-seedling period of tree peony, and disinfecting time is only 30 minutes before seed sand Tibetan of the present invention, seed soaking normal-temperature water, seed soaking time is no more than 12 hours, oil is 15-16 week with the growing-seedling period of tree peony, and centre does not need indoor low temperature breaking dormancy.
(2) rooting rate is improved.The rooting rate of ornamental peonies ' Paeonia delavayi ' is 91.11%-94.95%, and the present invention's oil is more than 99% with the rooting rate of tree peony.
(3) emergence rate is improved.The emergence rate of ornamental peonies ' Paeonia delavayi ' is 89.77%-96.89%, and oil is only 49% with the emergence rate of the conventional field seedling raising technology of tree peony ' Paeonia papaveracea ', and emergence rate of the present invention is more than 99%.
(4) planting percent is improved.Ornamental peonies ' Paeonia delavayi ' transplanting survival rate is 89.77%-96.89%, and the present invention adopts bag on bud seedling to transplant, and survival rate is high, and transplanting survival rate reaches more than 99%.
(5) seedling cost is reduced.The present invention does not need Soaking in hot water, does not need Low-temperature culture, and upper bag after seed germination, each non-woven bag 1 seed, has saved seed, greatly reduced production cost.
(6) Phase ofsprouting is improved.Oil adopts sowing in the field method with tree peony, and not only emergence rate is low, and it is irregular to emerge, and reduces commercial seedling quality, the seedling-cultivating method that the present invention adopts, and emergence rate is high, and emerge neat, seedlings root is flourishing, substantially increases Quality of Seedlings.
(7) seedling-cultivating method innovation.On the bud seedling that the present invention adopts, bag is explored with belonging in tree peony seedling growing process first at oil, and adopt seedling-cultivating method of the present invention, seed germination rate and planting percent all reach more than 99%, and growing-seedling period shortens about 90 days traditional nursery.
(8) accelerate industry and promote paces, become transplantation of seedlings not to be subject to seasonal restrictions.On bud seedling of the present invention, bag grows up to finished product seedling, can plant at any time and be not subject to seasonal restrictions according to industry development demand.
In a word, the present invention passes through collection and the process of oil Paeonia suffruticosa seed, seed storage, seedbed prepares, seeding and seedling raising, move bag on bud, after transplanting, management waits measure, shorten seedling raise period, improve breeding efficiency, save seedling cost, improve Phase ofsprouting and emergence rate, substantially increase seedling quality, on bud seedling, bag can not be subject to seasonal restrictions plantation after surviving, be conducive to oil developing by tree peony industry, rooting rate and germination rate are up to more than 99%, emerge neat, the seedlings root obtained is flourishing, fibrous root is many, bud seedling is sturdy, there is wide market application foreground.
Embodiment
Following examples further describe implementation process and the beneficial effect of the inventive method, embodiment is only for the object of illustration, do not limit the scope of the invention, the apparent change that those of ordinary skill in the art make according to the present invention is simultaneously also contained within the scope of the invention.In addition, reagent of the present invention, wherein:
GA
3chemical composition be gibberellin, be configured to high concentration mother liquor during use, i.e. 0.5mg/mL.Concrete configuration method: take 50 milligrams of GA
3, first dissolve with a small amount of 95% alcohol, be then settled to 100 milliliters with distilled water, can use.
The chemical composition of carbendazim is 2-benzimidazole ylcarbamic acid methyl ester, and be the efficient systemic fungicide of a kind of broad spectrum type low toxicity, used in the present invention is 50% carbendazol wettable powder, is configured to high concentration mother liquor during use, is 200 times of liquid.Concrete compound method: take 1g carbendazim, be settled to 200ml with clear water, can use.
Embodiment 1: seed soaking
The Paeonia papaveracea seed that on September 30th, 2014 will plant only by wet concentration method, carbendazol wettable powder with 50% 1000 times of liquid disinfectants 30 minutes, rinse clean with clear water, then soak seed by normal-temperature water, seed soaking time arranges 3 process, be respectively 12 hours, 24 hours, 48 hours, often process 100 seeds, repeat for 3 times.Pick up after process and spread shady and cool place and receive dry to planting crust.Seed is divided and is laid in the husky bed crossed of carbendazim wettable envelope agent 800 times of liquid disinfectants of 50%, every layer covers husky 4cm, and husky water content is 10%-15%, and the humidity of sand is to hold agglomerating not water outlet as well, the place of storage is common booth, and temperature range is 23-25 DEG C.Stratification time is 15 days, and situation of the taking root statistics of seed is as follows:
The impact that the different seed soaking time of table 1 is taken root on oil Paeonia suffruticosa seed
| Seed soaking time/h | Rooting rate/% | Main root length/cm |
| 12 | 99.33±0.33a | 1.61±0.11a |
| 24 | 99.00±0.58a | 1.67±0.07a |
| 48 | 99.33±0.33a | 1.77±0.14a |
Adopt the seed-soaking method of embodiment 1, the rooting rate of lamination oil Paeonia suffruticosa seed after 15 days all reaches more than 99%, and main root length difference is not remarkable.
Embodiment 2: husky Tibetan Stratification time
The Paeonia papaveracea seed that on September 30th, 2014 will plant only by wet concentration method, 1000 times of liquid disinfectants of the carbendazol wettable powder with 50% 30 minutes, rinse clean with clear water, pick up to spread shady and cool place and receive dry to planting crust after then soaking seed 12 hours by normal-temperature water.Seed is divided and is laid in the husky bed crossed of carbendazim wettable envelope agent 800 times of liquid disinfectants of 50%, every layer covers husky 4cm, and husky water content is 10%-15%, and the humidity of sand is to hold agglomerating not water outlet as well, the place of storage is common booth, and temperature range is 23-25 DEG C.Stratification time arranges 3 process, is respectively 5 days, 10 days, 15 days, often processes 100 seeds, repeats for 3 times.Situation of the taking root statistics of seed is as follows:
The different husky impact of hiding Stratification time and oil Paeonia suffruticosa seed being taken root of table 2
| Husky Tibetan Stratification time/d | Rooting rate/% |
| 5 | 30.00±3.79a |
| 10 | 87.67±2.40b |
| 15 | 99.67±0.33c |
Adopt the sand of embodiment 2 to hide lamination method, the rooting rate of lamination oil Paeonia suffruticosa seed after 15 days all reaches more than 99%, and rooting rate hides 5 days and 10 days apparently higher than sand.
Embodiment 3: seeding and seedling raising
On October 13rd, 2014, hide the seed gibberellin of plant growth regulator (GA of taking root for 15 days by through husky
3) immersion treatment, process arranges concentration and is respectively 100ppm, 150ppm, 200ppm, processing time is set to 12 hours, 24 hours, 48 hours, be sowed at after process through 50% the husky bed of nursery crossed of carbendazim wettable envelope agent 800 times of liquid disinfectants (early September selects physical features smooth near nursery lot, husky bed is built above excessively drained field, husky bed lime brick is built into, husky bedside 1.0-1.2m, length is because determining in selected plot, from ground, height is up about 20cm, husky thick 16-18cm, seedbed spacing is 40cm, filling sand is fresh river sand, rugosity is 0.3mm.) in, adopt the mode of drilling, the seed (the long 1.5-2.0cm of root) of Sha Zanghou is seeded in husky bed, seed is sprinkled upon in ditch uniformly, line-spacing 6cm, ditch depth 2-3cm, then cover husky lid put down and cover with thin straw screen or mat, use watering can trickle, drench every day and ensure that seedbed is moistening, shelters from heat or light with the sunshade net of 60% above seedbed for 1-2 time.Seed started to sprout February 15, and reached February 22 and sprout peak, germination rate situation is as follows:
The impact that table 3 variable concentrations HORMONE TREATMENT different time is sprouted with Paeonia suffruticosa seed oil
Embodiment 3 variable concentrations is adopted to process the seedling-cultivating method of different time rapidly, oil is that 150ppm soaks seed more than 12 hours with tree peony Paeonia papaveracea seed in gibberellin concentration, germination rate can reach more than 90%, wherein gibberellin concentration is 150ppm process 12 hours and 24 hours, and the germination rate of oil Paeonia suffruticosa seed can reach 99%.
Embodiment 4: upper bag of transplanting seedlings
Upper bag of transplanting seedlings is provided with 3 periods, and period 1 grows to 1.5-2.0cm for bud seedling, shows as cotyledon and displays for the first time but do not launch, and plants shell and does not come off; Period 2 is about 2.5-3.0cm for bud seedling is long, and cotyledon just launches, and plants shell and comes off; Period 3 grows 2-3 sheet true leaf for bud seedling.Each process 30 bud seedlings, repeat for 3 times.Concrete grammar is: play bud seedling before 10 AM or after at 3 in afternoon, pulls up bud seedling wet towel or wet newspaper covering moisturizing, pack in time with bamboo let is auxiliary.Transplanting container used is biological degradability non-woven bag, and cylindrically, be highly 16cm, upper opening place bore is 12cm.Matrix used is V (peat): V (perlite): V (vermiculite): V (chicken manure)=6:1:1:2, matrix is through 50% carbendazol wettable powder, 800 times of liquid disinfectants, and the water content of matrix remains on 30% weight to 60% weight.Moving bud waters permeable by the container filling matrix before noon, and sufficient water also will be drenched in bud seedbed.Insert container bag central authorities with a thin bamboo let after moving bud, holding bud seedling and put into cave, leaning on soil with 45° angle to bud seedling from 1.5cm-2.0cm place, hole bamboo let, bud shoot root portion being tightly combined with soil.Each container field planting 1 strain tree peony bud seedling.Transplant rear timely pouring normal root water.After bud transplantation of seedlings enters container, put neat, surrounding river sand encloses and blocks, and shelters from heat or light with the sunshade net that shading rate is 75%, sprinkle water 1-2 time first 10 day every day after firm transplanting, keep moistening, trickle 1-2 time weekly after 10 days, does not do and does not water, water, irrigate, grow after 2-3 sheet true leaf until seedling, spray disinfectant liquid disinfectant, within every 10 days, spray 1 time.Transplant and check planting percent after 30 days, concrete condition statistics is as follows:
The different Different Transplanting Periods of table 4 is on the impact of oil with tree peony seedling planting percent
| Different Transplanting Periods | Planting percent |
| 1 | 99.67±0.33c |
| 2 | 96.33±0.88b |
| 3 | 89.00±1.15a |
Adopt the method for embodiment 4, point 3 periods move bud seedling and enter non-woven bag, wherein Different Transplanting Periods 1 and period 2 planting percent can reach more than 96%, transplant planting percent in period 1 the highest, can more than 99% be reached.
Embodiment 5: seedling bed is tested
According to the operation of example 1-4, wherein the clean seed of purple plague purpura with 50% carbendazol wettable powder 1000 times of liquid disinfectants 30 minutes, rinse only with clear water and soak seed 12 hours by normal-temperature water, point to be laid in the husky bed crossed of carbendazim wettable envelope agent 800 times of liquid disinfectants of 50%, every layer covers husky 4cm, husky water content is 10%-15%, the humidity of sand is to hold agglomerating not water outlet as well, the place of storage is common booth, temperature range is 23-25 DEG C, within 15 days, is the gibberellin of plant growth regulator (GA of 150ppm by earthing of seeds afterwards by concentration
3) soak 12 hours, be sowed at respectively through 50% the husky bed of nursery crossed of carbendazim wettable envelope agent 800 times of liquid disinfectants and native bed in, native bed is identical with the specification of husky bed, respectively broadcasts 100, repeats for 3 times.Situation of specifically emerging statistics is as follows:
The different seedling bed of table 5 is on the impact of oil seed germination
| Seedling bed | Start sprout time | Germination rate/% |
| Husky bed | On February 15th, 2015 | 99.33 |
| Soil bed | On February 23rd, 2015 | 68.67 |
Adopt husky bed and the nursery respectively of native bed of embodiment 5, the germination rate of husky bed seed is 99.33%, and native bed is only 68.67%, and husky bed seed germination will be sprouted prior to native bed for one week.
Claims (10)
1. the oil seeding growing seedlings method of tree peony, the method comprises the collection of oil Paeonia suffruticosa seed and process, seed storage, seedbed prepare, seeding and seedling raising, move bag on bud, transplant and manage afterwards, and concrete steps are as follows:
1) seed collection and process: to gather mellow fruit (planting the fruit that pod is buff) in mid or late August, shady and cool moist place is deposited in after gathering, every day is stirred 1-2 time, in order to avoid heating, allow seed physiological ripening in kind of pod, when the fruit of more than 85% is dry and cracked, seed is deviate from naturally, only plants before seed storage by wet concentration method;
2) seed storage: storage environment is in conventional booth, temperature range is 20 DEG C-28 DEG C; Before storage, pure seed is sterilized, rinse well with clear water, then with normal-temperature water seed soaking 10-12 hour, spreading shady and cool place receives dry to planting crust, seed place Min layers is placed in the wet sand after disinfecting and preserves, every layer covers husky 3-5cm, and wet husky water content is 10%-15% weight, and the husky Tibetan time is 10-15 days;
3) seedbed prepares: seedling bed adopts husky bed, is cut into lime brick, and filling sand is fresh river sand;
4) seeding and seedling raising: by step 2) after the seed of Sha Zanghou soaks with plant growth regulator, be sowed in the husky bed of sterile nursery, adopt the mode of drilling, broadcast rear thin straw screen or mat to cover, trickle keeps seedbed moistening, shelters from heat or light, until seed germination above seedbed with sunshade net;
5) bag on bud is moved: display for the first time when seed shows as cotyledon on husky bed, lifting moves bud, moves to and is equipped with in the non-woven bag of matrix, field planting, the sufficient normal root water of rear pouring of having planted;
6) management after transplanting: after bud transplantation of seedlings enters container, put neat, surrounding river sand encloses and blocks, and shelters from heat or light with sunshade net, trickle moisturizing, grows after 2-3 sheet true leaf, spray disinfectant liquid disinfectant until seedling.
2. the oil seeding growing seedlings method of tree peony according to claim 1, is characterized in that: step 2) in be 50% carbendazol wettable powder, 1000 times of liquid to pure seed disinfectant used of sterilizing before storage, disinfecting time is 30 minutes.
3. the oil seeding growing seedlings method of tree peony according to claim 1, is characterized in that: step 2) in be 50% carbendazol wettable powder, 800 times of liquid to wet sand sterilization disinfectant used before storage, disinfecting time is 30 minutes to 60 minutes.
4. the oil seeding growing seedlings method of tree peony according to claim 1, is characterized in that: step 3) in husky thick 16-18cm in the husky bed that adopts of nursery, seedbed spacing is 30-50cm, and the rugosity of river sand used is 0.2mm-0.5mm.
5. the oil seeding growing seedlings method of tree peony according to claim 1, wherein step 4) in husky hide after seed be through the band root seed of the long 1.5cm-2.0cm of root after husky Tibetan.
6. the oil seeding growing seedlings method of tree peony according to claim 1, is characterized in that: step 4) in plant growth regulator used be gibberellin (GA
3), concentration is 150mg/L, and seed soak time is 12 hours.
7. the oil according to claim 1 seeding growing seedlings method of tree peony, it is characterized in that: step 5) in matrix used be the peat of volume ratio=6:1:1:2, perlite, vermiculite and chicken manure, through 50% carbendazol wettable powder, 800 times of liquid disinfectants, the water content of matrix remains on 30% weight to 60% weight.
8. the oil according to claim 1 seeding growing seedlings method of tree peony, it is characterized in that: step 6) in the shading rate of sunshade net used be 70-80%, sprinkle water 1-2 time first 10 day every day after firm transplanting, keep moistening, trickle 1-2 time weekly after 10 days, do not do and do not water, water, irrigate.
9. the oil seeding growing seedlings method of tree peony according to claim 1, is characterized in that: step 6) in thimerosal used be 50% carbendazol wettable powder, 1000 times of liquid, within every 10 days, spray 1 time.
10., according to any one of the claim 1-9 oil seeding growing seedlings method of tree peony, it is characterized in that: described oil Paeonia suffruticosa seed is the seed of Paeonia papaveracea PaeoniaRockii.
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