CN108370678A - The method for promoting David's-harp seed to sprout - Google Patents
The method for promoting David's-harp seed to sprout Download PDFInfo
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- CN108370678A CN108370678A CN201810013836.3A CN201810013836A CN108370678A CN 108370678 A CN108370678 A CN 108370678A CN 201810013836 A CN201810013836 A CN 201810013836A CN 108370678 A CN108370678 A CN 108370678A
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- seed
- david
- harp
- soaking
- seeds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Abstract
The invention discloses a kind of methods that promotion David's-harp seed is sprouted, and include the following steps successively：David's-harp seed is cleaned, to remove the impurity of the surface of the seed；Seed after cleaning is hoted water treatment of seeds successively, plant hormone seed soaking；The plant hormone is soaked seed：First by the seed after hoting water treatment of seeds in 40 ± 5 DEG C of KNO in mass concentration 0.5~1.5%324 ± 2h of solution seed soaking, then flowing water flushing place into 12 ± 1h of seed soaking in hormone liquid；The seed of gained is in moistening, 25 ± 1 DEG C of dark culturings after above-mentioned seed soaking, to make germination.This method can break the suspend mode of more chrysanthemum essence seeds, promote the sprouting of seed, shorten the growth year of sealwort, improve medicinal herb grower's income.
Description
Technical field
The present invention relates to a kind of methods that promotion David's-harp seed is sprouted.
Background technology
David's-harp (Polygonatum cyrtonema Hua.) is Liliaceae Polygonatum herbaceos perennial, collects medicine
With, edible, ornamental and cosmetic values.Sealwort is mild-natured, sweet in flavor, for nourish top grade, have nourishing Yin and moistening dryness, tonifying kidney and benefiting sperm,
Reducing blood lipid hypoglycemic, blood pressure lowering, improves the immunity of the human body, anti-aging and other effects, in terms of developing new drug and exploitation health products
With bright prospects.The characteristic in view of David's-harp with " integration of drinking and medicinal herbs ", with going deep into for health idea, market need in recent years
It asks and rises year by year.Currently, rely primarily on wild rhizome and seed in production to breed, due to the market demand is big, price year by year on
It rises, medicinal herb grower is unwilling that introduces a collection is selected to reserve seed for planting.Sealwort seed nature germination percentage is extremely low, to pass through very long rest period, the 1st year
Emergence rate is low, and 1 blade can be just grown after undergoing 2 winter dormancies, is sowed 2~3 years emerge using seed in production at present,
It is up to 5~6 years from harvesting is seeded into, effectively breaks the suspend mode of sealwort seed thus, it will help shorten the growth year of sealwort.
Correlative study is carried out to sealwort dormancy characteristics of different, the results showed that：The factors such as intercellular space is small, and water absorbing properties are poor
Restrict the normal sprouting of sealwort seed.It is such as de- that there are the high activity inhibiting substances of different content in sealwort seed, kind skin, endosperm
Fall acid, in sealwort seed maturity growth course, fruit and Interior Seed ABA contents change in " high-low-high ", seed at
Reach higher level after ripe again, so as to cause seed dormancy.Any of the above factor interaction regulates and controls sealwort seed jointly
Sprouting.Therefore it is very urgent to screen a kind of technology that promotion sealwort seed is sprouted, on the one hand can promote sealwort system industrialization
Propulsion, on the other hand the medical value of sealwort can be made adequately to be developed.
Invention content
The technical problem to be solved in the present invention is to provide a kind of method that promotion David's-harp seed is sprouted, this method can be beaten
The suspend mode for breaking more chrysanthemum essence seeds, promotes the sprouting of seed, shortens the growth year of sealwort, improves medicinal herb grower's income.
In order to solve the above technical problem, the present invention provides a kind of methods that promotion David's-harp seed is sprouted, including with
Lower step：
1), David's-harp seed is cleaned, to remove the impurity of the surface of the seed；
2), seed after the cleaning obtained by step 1) is hoted water treatment of seeds successively, plant hormone seed soaking；
It is described hot water treatment of seeds for：It seed will soak seed in 40 ± 5 DEG C of water 24 ± 2h after cleaning；
The plant hormone is soaked seed：By the seed after hoting water treatment of seeds in 40 ± 5 DEG C in mass concentration 0.5~1.5%
KNO3Soak seed 24 ± 2h in solution；
3) seed obtained by step 2), is made into germination in moistening, 25 ± 1 DEG C of dark culturings.
Incubation time is generally 45 ± 5 days.
The improvement of the method for promoting the sprouting of David's-harp seed as the present invention：
The plant hormone is soaked seed：First by the seed after hoting water treatment of seeds in 40 ± 5 DEG C in mass concentration 0.5~1.5%
KNO324 ± 2h of solution seed soaking, then flowing water flushing place into 12 ± 1h of seed soaking in hormone liquid；
The hormone liquid be it is following any one：The ethephon solution of 100~300mg/L (preferably 300mg/L), 150
Gibberellic acid (the GA of~250mg/L (preferably 200mg/L)3) solution, 100~500mg/L (preferably 300mg/L) sodium nitroprussiate
(SNP) solution.
Remarks explanation：To carry out at normal temperatures, the room temperature is generally 5~30 DEG C for seed soaking in hormone liquid.
The further improvements in methods for promoting the sprouting of David's-harp seed as the present invention：
The step 1) is：Get colors and in the same size, full seed, without the David's-harp seed of scar of going mouldy, in
Flowing water flushing is carried out under the conditions of rubbing, washing time is 25~35 minutes.
The further improvements in methods for promoting the sprouting of David's-harp seed as the present invention：
The step 3) is：Seed obtained by step 2) is placed in the culture dish for being covered with two layers of moistening filter paper, using 25
± 1 DEG C of constant incubator dark culturing keeps filter paper moistening in incubation.
Generally, for a diameter of 9cm culture dishes, about 15 seeds are placed in each culture dish.
The further improvements in methods for promoting the sprouting of David's-harp seed as the present invention：
The step 2) is：40 DEG C of seed elder generation lukewarm water is soaked seed for 24 hours after cleaning, then the 1%KNO at 40 DEG C3Solution seed soaking
For 24 hours, flowing water flushes three times, and places into the ethephon solution of 300mg/L the 12h that soaks seed.
The present invention promotion David's-harp seed germination method, can in the short time promote David's-harp seed sprouting,
And the purpose of Short-term Culture high quality seedling may be implemented by the tissue culture technology in laboratory.It in the method for the invention, will be more
The seed of flower sealwort can destroy the rigid shell of seed using lukewarm water, hormone seed soaking, and kind of skin is made to soften, and can quickly allow seed
It sprouts.Method using the present invention normally only needs to achieve the effect that within 60 days that a large amount of seeds are sprouted.So promotion of the invention
David's-harp seed germination method is a kind of method for not influenced by factors such as seasons, efficiently, quickly providing germination seed,
The cultivation period of sealwort can be shortened, improve planting rhizoma polygonati amount.
The inventive point of the present invention essentially consists in (but not limited to)：
1, the present invention promotes seed to sprout using the method for suitable temperature hoted water treatment of seeds, the results showed that hoting water treatment of seeds can make
Sealwort seed softens, and is more easy to absorb moisture and nutriment, while sterilizing seed, increases the resistance in later stage.
2, the present invention promotes the sprouting of sealwort seed, hormone seed soaking that can break stopping for sealwort seed using hormone seed soaking
It sleeps, the influence for avoiding temperature factor from sprouting seed.GA3As plant growth regulator, the generation of hydrolase is can induce, kind is made
Reserve substance in son is decomposed into small molecule from macromolecular, is easy to be utilized by embryo, to promote sprouting and the seedling of seed
Growth.
3, the present invention uses KNO3The method of solution seed soaking promotes seed to sprout, and potassium is the work of various important enzymes in plant
Agent promotes sugar transport, conversion and nitrogen metabolism, can activate seed dehydrogenase activity and increase respiratory intensity, adjust solution water
Gesture delays seed water absorption course, has time enough to carry out membranous system reparation and the activation of important enzyme system before so that seed is sprouted, improves
Intracellular environment and metabolism state, to promote seed to sprout.
4, the present invention promotes the sprouting of sealwort seed, the two to be jointly processed by, be more easy to using hormone seed soaking and water seed soaking
In the sprouting of more yellow sealwort seeds.
Specific implementation mode
With reference to specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in
This.
It in following embodiment, does not inform clearly, is in conventional progress at room temperature.
Embodiment 1-1, a kind of method for promoting David's-harp seed to sprout, follow the steps below successively：
1) it, gets colors and in the same size, full seed, the David's-harp seed without the scar that goes mouldy, under the conditions of rubbing
Carry out flowing water flushing, washing time 30min.
2), will hot water treatment of seeds for 24 hours in water of the seed prior to 40 DEG C after the cleaning obtained by step 1), then in 40 DEG C 1%
(quality %) KNO3It soaks seed in solution for 24 hours, flowing water flushes three times, and places into the ethephon solution of 300mg/L and soaks seed 12h (often
Temperature).
3), the seed of gained after step 2) processing is placed in the culture dish for the filter paper for being covered with two layers of moistening, is trained in constant temperature
25 DEG C of dark culturings of case are supported, filter paper moistening is kept.
Acquired results after culture 45 days：Germination percentage is 57.8%, germinating energy 46.7%, and foliation rate is 4.4%.
It is specific as follows：
Culture 14 days (2 weeks) is germinateed afterwards, and at 20 days, root long can reach 4cm, germination percentage 16.6%；At 23 days, grow
Lateral bud, at the 25th day, germination percentage 26.6% had root phenomenon of growing thickly, Some seeds to grow 2-3 radicle at the 29th day；The
At 33 days, more 5 lateral buds are grown to, have cotyledon to grow when cultivating 35 days (the 5th week), at the 45th day, lateral bud number is up to 10, root
Up to 7.5cm, fibrous root quantity is up to 5.
Embodiment 1-2, the concentration of the ethephon solution in embodiment 1-1 is made into 200mg/L by 300mg/L；Remaining etc.
It is same as embodiment 1-1.Acquired results are as described in Table 1 after culture 45 days.
Embodiment 1-3, the concentration of the ethephon solution in embodiment 1-1 is made into 100mg/L by 300mg/L；Remaining etc.
It is same as embodiment 1-1.Acquired results are as described in Table 1 after culture 45 days.
Embodiment 2, a kind of method for promoting David's-harp seed to sprout：
Cancel this link of 12h of soaking seed in the ethephon solution of 300mg/L in embodiment 1-1 steps 2)；
Remaining is equal to embodiment 1.
Acquired results after culture 45 days：Germination percentage is 46.67%, germinating energy 32.2%, and foliation rate is 3.3%.
It is specific as follows：
It shows money or valuables one carries unintentionally within 14th day, the 20th a length of 1.7cm of Tiangeng, the radicle of Some seeds is about 4cm or so at the 28th day, the 35th day
See cotyledon, the 45th day, for lateral bud number up to 5, root long 5.8cm, fibrous root quantity was up to 5.
Embodiment 3, a kind of method for promoting David's-harp seed to sprout：
Make the ethephon solution of 300mg/L in embodiment 1-1 steps 2) into 150mg/L, 200mg/L, 250mg/L respectively
Gibberellic acid (GA3) solution；Remaining is equal to embodiment 1.
Acquired results are as described in Table 1 after culture 45 days.
Embodiment 4, a kind of method for promoting David's-harp seed to sprout：
Make the ethephon solution of 300mg/L in embodiment 1-1 steps 2) into 100mg/L, 300mg/L, 500mg/L respectively
Sodium nitroprussiate (SNP) solution；Remaining is equal to embodiment 1.
Acquired results are as described in Table 1 after culture 45 days.
Embodiment 5-1, the step 2) in embodiment 1-1 is made into following change：KNO3Solution concentration is changed to 0.5%, remaining
It is equal to embodiment 1-1.
Acquired results after culture 45 days：Germination percentage is 44.4%, germinating energy 33.3%, and foliation rate is 2%.
Embodiment 5-2, the step 2) in embodiment 1-1 is made into following change：KNO3Solution concentration is changed to 1.5%, remaining
It is equal to embodiment 1-1.
Acquired results after culture 45 days：Germination percentage is 40%, germinating energy 35.8%, and foliation rate is 3.3%.
Comparative example 1, a kind of method for promoting David's-harp seed to sprout
Cancel 1%KNO in 1 step 2) of embodiment3Solution seed soaking this link for 24 hours：
Remaining is equal to embodiment 1.
Acquired results are：Acquired results after culture 45 days：Germination percentage is 46.67%, germinating energy 35.56%, foliation rate
It is 2.2%.
Comparative example 2, a kind of method for promoting David's-harp seed to sprout：
Cancel 1%KNO in 3 step 2) of embodiment3Solution seed soaking this link for 24 hours；Remaining is equal to embodiment 3.
Acquired results are as described in Table 1 after culture 45 days.
Comparative example 3, a kind of method for promoting David's-harp seed to sprout：
Cancel 1%KNO in 4 step 2) of embodiment3Solution seed soaking this link for 24 hours；Remaining is equal to embodiment 4.
Acquired results are as described in Table 1 after culture 45 days.
Comparative example 4, a kind of method for promoting David's-harp seed to sprout
Step 2) in embodiment 1-1 is changed to：Seed after cleaning obtained by step 1) is only subjected to 40 DEG C of seed soaking of lukewarm water
24h；
Remaining is equal to embodiment 1-1.
Cultivate 45 days acquired results：Germination percentage is 17.78%, germinating energy 8.89%, and foliation rate is 0.
Comparative example 5-1, the step 2) in embodiment 1-1 is made into following change：By KNO3Solution makes KCl solution, concentration into
It is constant；Remaining is equal to embodiment 1-1.
Acquired results after culture 45 days：Germination percentage is 16.7%, germinating energy 8.89%, and foliation rate is 0.
Comparative example 5-2, the step 2) in embodiment 1-1 is made into following change：By KNO3Solution makes KH into2PO4Solution is dense
It spends constant；Remaining is equal to embodiment 1-1.
Acquired results after culture 45 days：Germination percentage is 25.6%, germinating energy 12.11%, and foliation rate is 0.
Comparative example 6-1, by the step 2) KNO in embodiment 1-13The solution seed soaking time, remaining was equivalent by making 36h into for 24 hours
In
Embodiment 1-1.
Comparative example 6-2, by the step 2) KNO in embodiment 1-13The solution seed soaking time, remaining was equivalent by making 12h into for 24 hours
In
Embodiment 1-1.
Table 1
Finally, it should also be noted that it is listed above be only the present invention several specific embodiments.Obviously, this hair
Bright to be not limited to above example, acceptable there are many deformations.Those skilled in the art can be from present disclosure
All deformations for directly exporting or associating, are considered as protection scope of the present invention.
Claims (5)
1. the method for promoting David's-harp seed to sprout, including first cleans David's-harp seed, then by seed in moistening,
It is cultivated under 25 ± 1 DEG C of dark conditions；It is characterized in that：
Seed after cleaning is hoted water treatment of seeds successively, plant hormone seed soaking：
It is described hot water treatment of seeds for：It seed will soak seed in 40 ± 5 DEG C of water 24 ± 2h after cleaning；
The plant hormone is soaked seed：By the seed after hoting water treatment of seeds in 40 ± 5 DEG C of KNO in mass concentration 0.5~1.5%3It is molten
Soak seed 24 ± 2h in liquid；
The seed of gained after seed soaking is cultivated again.
2. the method according to claim 1 for promoting David's-harp seed to sprout, it is characterized in that：
The plant hormone is soaked seed：First by the seed after hoting water treatment of seeds in 40 ± 5 DEG C of KNO in mass concentration 0.5~1.5%3
24 ± 2h of solution seed soaking, then flowing water flushing place into 12 ± 1h of seed soaking in hormone liquid；
The hormone liquid be it is following any one：The ethephon solution of 100~300mg/L, the gibberellic acid of 150~250mg/L are molten
The sodium nitroprusside solution of liquid, 100~500mg/L.
3. the method according to claim 1 or 2 for promoting David's-harp seed to sprout, it is characterized in that：
Get colors and in the same size, full seed, without the David's-harp seed of scar of going mouldy, flowing water is carried out under the conditions of rubbing
It rinses, washing time is 25~35 minutes.
4. the method according to claim 1 or 2 for promoting David's-harp seed to sprout, it is characterized in that：
The culture is：The seed of gained after seed soaking will be placed in the culture dish for being covered with two layers of moistening filter paper, using 25 ± 1 DEG C
Constant incubator dark culturing keeps filter paper moistening in incubation.
5. the method according to claim 2 for promoting David's-harp seed to sprout, it is characterized in that：
40 DEG C of seed elder generation lukewarm water is soaked seed for 24 hours after cleaning, then the 1%KNO at 40 DEG C3For 24 hours, flowing water flushes three times solution seed soaking, then
The 12h that soaks seed is put into the ethephon solution of 300mg/L.
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| CN201810013836.3A CN108370678B (en) | 2018-01-08 | 2018-01-08 | Method for promoting germination of polygonatum cyrtonema seeds |
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| CN201810013836.3A CN108370678B (en) | 2018-01-08 | 2018-01-08 | Method for promoting germination of polygonatum cyrtonema seeds |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109496708A (en) * | 2018-12-19 | 2019-03-22 | 江西省鑫隆农业发展有限公司 | A kind of polygonatum cyrtonema seedling breeding method |
| CN110115131A (en) * | 2019-06-11 | 2019-08-13 | 丽水市农业科学研究院 | A kind of seedling method of polygonatum cyrtonema seed growing |
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| CN104838862A (en) * | 2015-05-23 | 2015-08-19 | 贵州大学 | Polygonatum multiflorum seed raising method |
| CN105230484A (en) * | 2015-09-25 | 2016-01-13 | 马龙县兴裕农业生物科技开发有限公司 | Rapid breeding method of rhizoma polygonati |
| CN106856723A (en) * | 2017-04-06 | 2017-06-20 | 凤冈县领益昕中药材种植有限公司 | The mating system of sealwort seed |
| CN107372591A (en) * | 2017-07-25 | 2017-11-24 | 王尧飞 | A kind of method of medicinal material Seed inducement germination |
-
2018
- 2018-01-08 CN CN201810013836.3A patent/CN108370678B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104838862A (en) * | 2015-05-23 | 2015-08-19 | 贵州大学 | Polygonatum multiflorum seed raising method |
| CN105230484A (en) * | 2015-09-25 | 2016-01-13 | 马龙县兴裕农业生物科技开发有限公司 | Rapid breeding method of rhizoma polygonati |
| CN106856723A (en) * | 2017-04-06 | 2017-06-20 | 凤冈县领益昕中药材种植有限公司 | The mating system of sealwort seed |
| CN107372591A (en) * | 2017-07-25 | 2017-11-24 | 王尧飞 | A kind of method of medicinal material Seed inducement germination |
Non-Patent Citations (1)
| Title |
|---|
| 张小玲 等: "《种子学基础》", 31 May 2016, 中国农业大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109496708A (en) * | 2018-12-19 | 2019-03-22 | 江西省鑫隆农业发展有限公司 | A kind of polygonatum cyrtonema seedling breeding method |
| CN110115131A (en) * | 2019-06-11 | 2019-08-13 | 丽水市农业科学研究院 | A kind of seedling method of polygonatum cyrtonema seed growing |
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