CN104380942B - A kind of processing method making long of Rhizoma Paridis seed four months, coming into leaves for eight months - Google Patents
A kind of processing method making long of Rhizoma Paridis seed four months, coming into leaves for eight months Download PDFInfo
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- CN104380942B CN104380942B CN201410502503.9A CN201410502503A CN104380942B CN 104380942 B CN104380942 B CN 104380942B CN 201410502503 A CN201410502503 A CN 201410502503A CN 104380942 B CN104380942 B CN 104380942B
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- rhizoma paridis
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
Abstract
The present invention relates to a kind of processing method making long of Rhizoma Paridis seed four months, coming into leaves for eight months, comprise the following steps: the first step, in selecting the Rhizoma Paridis seed capsules of maturation at the beginning of 9 months, make thinner and dry in the shade 30 days;Second step, under the Rhizoma Paridis seed cryogenic conditions that will dry in the shade at the beginning of 10 months place within 15 20 days, carry out K cryogenic treatment;3rd step, Rhizoma Paridis seed after completing K cryogenic treatment mid-October peel off capsule shell, by the Rhizoma Paridis seed broadcasting of band Folium Styracis Suberifoliae in ready Seedling dish, carry out seed germination;4th step, the Seedling dish of the 3rd step is placed in freezer, K cryogenic treatment 15 20 days;5th step, mid-January by Rhizoma Paridis seed broadcasting later for the 4th step K cryogenic treatment in the big Tanaka of height above sea level 2800 meters;6th step, carry out artificial weeder make, begin with Rhizoma Paridis seedling mid-April and come into leaves, have to mid-June the Rhizoma Paridis seedling of more than 85% to grow leaf, the method for the present invention makes the bud ratio of Rhizoma Paridis seed and planting percent reach more than 85%.
Description
Background technology
The present invention relates to a kind of plant cultivation method, especially one make long of Rhizoma Paridis seed four months, eight
The processing method come into leaves for individual month.
Background technology
Rhizoma Paridis plant is subordinate to Liliaceae Paris Linnaeus(Paris L.), is that multiple Chinese patent medicine such as YUNNAN BAIYAO, GONGXUENING, pyretic toxicity are clear
Deng primary raw material.Along with constantly widening of its purposes, the demand of Rhizoma Paridis resource also increases sharply.According to money
Material statistics display, at present, available Rhizoma Paridis resource nearly 80% has been developed that totally, remaining 20% resource
According to current international and domestic demand analysis, may maintain 3~5 years.Therefore, Rhizoma Paridis is carried out on a large scale medicinal
The plantation of plant has very important meaning.
The subject matter that the plantation of restriction Rhizoma Paridis plant scale at present produces is the shortage of Rhizoma Paridis seedling;Due to weight
Building plant is the embryo of seed still hypoplasia when fruit maturation, plumule, plumular axis, radicle and cotyledon in its embryo
The most do not grow formation, and endosperm is hard, form after-ripening and the physiological dormancy such as endotesta top layer keratinization
Characteristic so that Rhizoma Paridis seed typically requires two summers in winter one of experience after conventional field sowing and could sprout and emerge,
Time required for seed germination is longer, is typical " biennial seed ";And in its natural state, even if
Its emergence rate of Rhizoma Paridis seed that experienced by long-time dormancy is relatively low.
Have people at present and just break Rhizoma Paridis seed dormant art applications patent, as number of patent application is
The patent application document of 98118955.5 discloses one " Rhizoma Paridis germination and quick breeding technology thereof ", should
Technology is first to be soaked by the gibberellins of Rhizoma Paridis seed 20-80ppm, uses 4 DEG C to hand over 22 DEG C of alternating temperatures the most again
For cultivating, add hormone and Rhizoma Paridis oligosaccharide the most in the medium, so that seed the earliest can be at 2 months
Sprout, although this technology can effectively break the rest period of Rhizoma Paridis seed, shorten the seed germination time, but alternating temperature
Operating process is complicated, and finally need to access in the culture medium containing hormone and Rhizoma Paridis oligosaccharide, production cost
High.
It was all to make the long root of Rhizoma Paridis seed in 1 year that other breaks the method reality of Rhizoma Paridis kind dormancy germination,
But after germination, Second Year just can come into leaves and become seedling, the purpose come into leaves within not reaching a year.
Summary of the invention
The invention provides a kind of processing method making long of Rhizoma Paridis seed four months, coming into leaves for eight months, solve
Determine the problems such as existing Rhizoma Paridis seedling raise period length, bud ratio be low.
For solving above-mentioned technical problem, the technical solution used in the present invention is:
A kind of processing method making long of Rhizoma Paridis seed four months, coming into leaves for eight months, comprises the following steps:
The first step, in select at the beginning of 9 months maturation Rhizoma Paridis seed capsules, standard be 85% capsule peel cracking,
Make thinner and dry in the shade 30 days, dry out wilting to red seed coat;
Second step, under the Rhizoma Paridis seed cryogenic conditions that will dry in the shade at the beginning of 10 months place within 15-20 days, carry out low temperature
Process;
3rd step, Rhizoma Paridis seed capsules after completing K cryogenic treatment mid-October peel off shell, carry red
Skin is sown in ready Seedling dish, carries out seed germination, temperature: 18-20 DEG C, the time: 85-95 days, has
More than 95% Rhizoma Paridis seminal root grows to 1-2 centimeter length;
4th step, the Seedling dish of the 3rd step is placed in freezer, K cryogenic treatment 15-20 days;
5th step, mid-January by Rhizoma Paridis seed broadcasting later for the 4th step K cryogenic treatment in height above sea level 2800
The big Tanaka of rice;
6th step, carry out artificial weeder make, begin with Rhizoma Paridis seedling mid-April and come into leaves, to mid-June
The Rhizoma Paridis seedling having more than 85% grows leaf.
Further, a preferred embodiment of the present invention is: the temperature of the described K cryogenic treatment described in the first step
Degree is for 2-4 DEG C, and the time is: 15 days.
Further, a preferred embodiment of the present invention is: the temperature of the described K cryogenic treatment described in the 4th step
Degree is for 2-4 DEG C, and the time is: 15 days.
Further, a preferred embodiment of the present invention is: the 3rd described step seed germination is particularly as follows: in 10
Rhizoma Paridis seed capsules after completing K cryogenic treatment the middle of the month peels off shell, and band Folium Styracis Suberifoliae is sown in ready
In Seedling dish, Seedling dish is first put thin nylon gauze, then put sterilized, the fertile soil of parasite killing, gross thickness 5 lis
Rice, earthing 1.5 centimetres on Rhizoma Paridis seed, density is that every 2 square centimeters of Rhizoma Paridis seeds are advisable;Root
A water within every 5-7 days, is watered according to fertile soil dry and wet situation.
Further, a preferred embodiment of the present invention is: the 5th described step is particularly as follows: incite somebody to action mid-January
Thin nylon gauze in Seedling dish is mentioned, by with Rhizoma Paridis seed broadcasting humous in the land for growing field crops of height above sea level 2800 meters
In, density is 100,000 every mu, the loam after seed covers 2 centimetres, covers the pine of 2 cm thicks the most again
Pin;According to soil moisture degree, within 10-15 days, water once.
Beneficial effects of the present invention:
1. dry in the shade by making thinner, allow Rhizoma Paridis seed fully absorb the nutrition of episperm, in promoting Rhizoma Paridis embryo
The morphogenesis of each organ and reaching maturity, promotes that in Rhizoma Paridis seed, reserve substance endosperm converts the most further
For availability status, the division of supply seed germination initial stage blastocyte and the needs of orga-nogenesis, complete in time
The form of Rhizoma Paridis seed and physiology after-ripening.Existing correlation process method patent is all by the red crust of Rhizoma Paridis seed
Remove, have impact on the nutrition of Yunnan the most always neutron absorption redness seed coat, cause Rhizoma Paridis percentage of seedgermination low,
Germinating energy is weak, germination is slow.
2. simulation nature winter low temperature environment (K cryogenic treatment for the first time) so that Rhizoma Paridis seed is beaten effectively
Broken dormancy, makes Rhizoma Paridis seed enter the long root stage from resting state.
3., by carrying out the research that Rhizoma Paridis root growth speed is affected by temperature, keep its fastest speed of growth pair
The temperature answered, makes Rhizoma Paridis root keep the fastest speed of growth by i.e. 18-20 degree Celsius.
The most again the Rhizoma Paridis seed sent out roots is positioned under low temperature, i.e. simulates through the second winter, again beat
Broken dormancy.The Rhizoma Paridis method for treating seeds major part of present patented mandate does not carry out second time low temperature mould
Intend the process of the second winter, and the second time K cryogenic treatment carried out after the long root of Rhizoma Paridis seed decides Rhizoma Paridis seed
Can realize 1 year coming into leaves.And emerging of Rhizoma Paridis seed refer to Rhizoma Paridis seed and send out roots and leaf,
Other correlational studyes are thought Rhizoma Paridis to send out roots i.e. to emerge, and make the long root of Rhizoma Paridis seed in unrealized 1 year
The purpose come into leaves.
5. place thin nylon gauze in Seedling dish and can realize quickly taking out from Seedling dish the purpose of Rhizoma Paridis seedling,
And avoid Rhizoma Paridis seedling root growth long, the injury taking little transplantation of seedlings when, root caused.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to enforcement
In example or description of the prior art, the required accompanying drawing used is briefly described, it should be apparent that, describe below
In accompanying drawing be only some embodiments of the present invention, for those of ordinary skill in the art, do not paying
On the premise of going out creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is that Rhizoma Paridis seed is taken root figure;
Fig. 2 is that Rhizoma Paridis seed comes into leaves seedling figure.
Detailed description of the invention
Technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that retouched
The embodiment stated is only a part of embodiment of the present invention rather than whole embodiments.Based in the present invention
Embodiment, those of ordinary skill in the art obtained under not making creative work premise all its
His embodiment, broadly falls into the scope of protection of the invention.
Embodiment 1
A kind of processing method making long of Rhizoma Paridis seed four months, coming into leaves for eight months, comprises the following steps:
The first step, in select at the beginning of 9 months maturation Rhizoma Paridis seed, standard be 85% capsule peel cracking, stand
Thin dry in the shade 30 days, dry out wilting to red seed coat;
Second step, place under the Rhizoma Paridis seed cryogenic conditions that will dry in the shade at the beginning of 10 months and carry out at low temperature for 15 days
Reason, cryogenic temperature is: 2-4 DEG C;
3rd step, the 3rd described step seed germination are particularly as follows: after completing K cryogenic treatment mid-October
Rhizoma Paridis seed capsules peels off shell, the Rhizoma Paridis seed broadcasting of band Folium Styracis Suberifoliae in ready Seedling dish, Seedling dish
Middle first put thin nylon gauze, then put sterilized, the fertile soil of parasite killing, gross thickness 5 centimetres, Rhizoma Paridis kind
Earthing 1.5 centimetres on son, density is that every 2 square centimeters of Rhizoma Paridis seeds are advisable;Temperature: 18-20 DEG C,
Time: 90 days, more than 85% Rhizoma Paridis seminal root is had to grow to 1-2 centimeter length;According to fertile soil dry and wet feelings
Condition waters a water for every 5-7 days;
4th step, being placed in freezer by the Seedling dish of the 3rd step, K cryogenic treatment 15 days, cryogenic temperature is: 2-4 DEG C;
5th step, mid-January, the thin nylon gauze in Seedling dish is mentioned, Rhizoma Paridis kind humous will be carried
Son broadcasts sowing in the big Tanaka of height above sea level 2800 meters, and density is 100,000 every mu, after seed covers 2 centimetres
Loam, covers the Folium Pini of 2 cm thicks the most again;According to soil moisture degree, within 10-15 days, water once.
6th step, carry out artificial weeder make, begin with Rhizoma Paridis seedling mid-April and come into leaves, to mid-June
The Rhizoma Paridis seedling having more than 85% grows leaf.
Embodiment 2
Substantially the same manner as Example 1, difference is: the K cryogenic treatment time in second step is 20 days, the
Time in three steps is 85 days, and the K cryogenic treatment in the 4th step is 18 days.
Embodiment 3
Substantially the same manner as Example 1, difference is: the K cryogenic treatment time in second step is 15 days, the
Time in three steps is 95 days, and the K cryogenic treatment in the 4th step is 20 days.
Embodiment 4
Substantially the same manner as Example 1, difference is: the K cryogenic treatment time in second step is 18 days, the
Time in three steps is 90 days, and the K cryogenic treatment in the 4th step is 15 days.
Embodiment 5
Substantially the same manner as Example 1, difference is: in selecting ripe Rhizoma Paridis seed at the beginning of 9 months, remove
Remove red seed coat.
Embodiment 6
Substantially the same manner as Example 1, difference is: do not carry out the 4th step, directly by the Yunnan weight of the 3rd step
Building planting seed.
Embodiment 7
Substantially the same manner as Example 1, difference is: the cryogenic temperature in second step is: 0 DEG C.
Embodiment 8
Substantially the same manner as Example 7, difference is: the cryogenic temperature in the 4th step is: 0 DEG C.
Embodiment 9
Substantially the same manner as Example 1, difference is: in the 3rd step need not carefully nylon gauze, directly by Yunnan
Rhizoma Paridis seed is placed on fertile soil.
The germination percentage of Rhizoma Paridis seed and the situation of planting percent of embodiment 1-9 are shown in Table following table.
Germination percentage refers to number and the percentage ratio of total number seeds that Rhizoma Paridis seed germination sends out roots;
Planting percent refers to that Rhizoma Paridis seed sends out roots and the number of leaf and the percentage ratio of total number seeds.
The germination percentage of the different embodiment Rhizoma Paridis seed of table 1 and emergence rate
Germination percentage (%) | Emergence rate (%) | |
Embodiment 1 | 95 | 93 |
Embodiment 2 | 90 | 87 |
Embodiment 3 | 93 | 90 |
Embodiment 4 | 95 | 91 |
Embodiment 5 | 75 | 60 |
Embodiment 6 | 90 | 20 |
Embodiment 7 | 80 | 70 |
Embodiment 8 | 75 | 60 |
Embodiment 9 | 92 | 85 |
As shown in Table 1, use the germination percentage of the method Rhizoma Paridis seed of the present invention and emergence rate the highest, reach
More than 85%.From Fig. 1 and 2, Rhizoma Paridis seed is taken root and the seedling that comes into leaves has essential difference, especially
Be Rhizoma Paridis seed take root to the seedling that comes into leaves need experience long time.
From embodiment 5, drying in the shade of Rhizoma Paridis seed processes the germination percentage to Rhizoma Paridis seed and the shadow of emergence rate
Ring very big, dry in the shade by making thinner, allow Rhizoma Paridis seed fully absorb the nutrition of red episperm, promote Yunnan weight
The morphogenesis of each organ and reaching maturity in building embryo, promotes reserve substance in Rhizoma Paridis seed the most further
Endosperm is converted into availability status, in time the division of supply seed germination initial stage blastocyte and the need of orga-nogenesis
Want, complete sprouting and the leave of form and the physiology after-ripening of Rhizoma Paridis seed, beneficially Rhizoma Paridis seed.
From embodiment 6, without twice K cryogenic treatment, only with a K cryogenic treatment, Rhizoma Paridis seed
Germination percentage the highest, but emergence rate is the lowest, it is impossible to solve 2 years seedlings of Rhizoma Paridis seed problem.
From embodiment 7 and 8, the temperature of K cryogenic treatment is for the germination percentage of Rhizoma Paridis seed and emergence rate
Substantially, too low temperature and long-time K cryogenic treatment can affect the sprouting of Rhizoma Paridis seed in impact.
From embodiment 9, do not use thin nylon gauze to protect Rhizoma Paridis seed, the Rhizoma Paridis seed taken root can be made
In seeding process, occur that root is damaged, reduce the emergence rate of Rhizoma Paridis seed.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all the present invention's
Any amendment of being made in protection domain, equivalent etc., should be included within the scope of the present invention.
Claims (3)
1. the processing method making long of Rhizoma Paridis seed four months, coming into leaves for eight months, it is characterised in that include following step
Rapid:
The first step, in select at the beginning of 9 months maturation Rhizoma Paridis seed, standard be 85% capsule peel cracking, make thinner and dry in the shade 30 days, extremely
Red seed coat dries out wilting;
Second step, carry out K cryogenic treatment in being placed under the conditions of 2-4 DEG C 15 days by the Rhizoma Paridis seed dried in the shade at the beginning of 10 months;
3rd step, Rhizoma Paridis seed after completing K cryogenic treatment mid-October peel off capsule shell, and seed coat broadcasts sowing redly
In ready Seedling dish, carry out seed germination, temperature: 18-20 DEG C, the time: 85-95 days, have more than 95% Rhizoma Paridis seminal root
Grow to 1-2 centimeter length;
4th step, the Seedling dish of the 3rd step is placed in freezer, K cryogenic treatment 15 days under the conditions of 2-4 DEG C;
5th step, mid-January by Rhizoma Paridis seed broadcasting later for the 4th step K cryogenic treatment in the big Tanaka of height above sea level 2800 meters;
6th step, carry out artificial weeder make, begin with Rhizoma Paridis seedling mid-April and come into leaves, have more than 85% to mid-June
Rhizoma Paridis seedling grows leaf.
A kind of processing method making long of Rhizoma Paridis seed four months, coming into leaves for eight months the most according to claim 1, it is special
Levy and be: the 3rd described step seed germination is particularly as follows: the Rhizoma Paridis seed after completing K cryogenic treatment mid-October is peelled off
Capsule shell, during seed coat is sown in ready Seedling dish redly, first puts thin nylon gauze in Seedling dish, then put sterilized, kill
The fertile soil of worm, gross thickness 5 centimetres, earthing 1.5 centimetres on Rhizoma Paridis seed, density is every 2 square centimeters of Rhizoma Paridis kinds
Son;A water within every 5-7 days, is watered according to fertile soil dry and wet situation.
A kind of processing method making long of Rhizoma Paridis seed four months, coming into leaves for eight months the most according to claim 2, it is special
Levy and be: the 5th described step, particularly as follows: mentioned by the thin nylon gauze in Seedling dish mid-January, will carry Yunnan humous weight
Building seed broadcasting is in the big Tanaka of height above sea level 2800 meters, and density is 100,000 every mu, the loam after seed covers 2 centimetres, then
Cover the Folium Pini of 2 cm thicks again;According to soil moisture degree, within 10-15 days, water once.
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CN1258966C (en) * | 2003-08-02 | 2006-06-14 | 腾冲县生物技术开发有限责任公司 | Artificial Rhizoma paridis seedling growing process |
CN1258964C (en) * | 2004-11-01 | 2006-06-14 | 成都中康光大药业有限公司 | Wild Paris chinensis Franch artificial domestication grow seedling and cultivation method |
CN101189943A (en) * | 2006-11-18 | 2008-06-04 | 怀化医学高等专科学校 | Seed propagating fast breeding method for paris polyphylla |
CN101822157B (en) * | 2010-04-21 | 2011-07-27 | 云南省农业科学院药用植物研究所 | Paris polyphylla var. yunnanensis scale seedling culturing method |
CN201976509U (en) * | 2010-09-07 | 2011-09-21 | 长江大学 | Crop seedling plate with water retention and bare-root resistant functions |
CN102835207B (en) * | 2012-09-19 | 2014-01-29 | 贵州三泓药业股份有限公司 | Method for reducing sleeping cycle of Paris polyphylla seeds |
CN103609223B (en) * | 2013-08-06 | 2015-09-02 | 中南民族大学 | Gibberellin and wet sand bed amass the method that paris polyphylla seed quick-speed germination is bred |
CN103548514A (en) * | 2013-10-22 | 2014-02-05 | 香格里拉县富盛达生物科技有限责任公司 | Selective breeding method of Yunnan manyleaf paris rhizome high-altitude cultivated species |
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