CN107372591A - A kind of method of medicinal material Seed inducement germination - Google Patents

A kind of method of medicinal material Seed inducement germination Download PDF

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Publication number
CN107372591A
CN107372591A CN201710613915.3A CN201710613915A CN107372591A CN 107372591 A CN107372591 A CN 107372591A CN 201710613915 A CN201710613915 A CN 201710613915A CN 107372591 A CN107372591 A CN 107372591A
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seed
agent
medicinal material
facilitates
soaked
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王尧飞
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N33/00Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
    • A01N33/16Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
    • A01N33/18Nitro compounds
    • A01N33/20Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group
    • A01N33/22Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group having at least one oxygen or sulfur atom and at least one nitro group directly attached to the same aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/12Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/22Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Toxicology (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Soil Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of method of medicinal material Seed inducement germination, by the way that first medicinal material seed kind skin is removed, its mesosperm produces first by ultraviolet irradiation in syrup and facilitates agent, seed is soaked in second be made up of brassin lactones, compound sodium nitrophenolate, amine fresh fat, ammonium nitrate, potassium nitrate, 6 benayl aminopurines and facilitated in agent first, described first is soaked in again facilitates process in agent, the medicinal material seed germination cycle can significantly be shortened, promote germination, artificial growth medical material quanlity is significantly improved, reduces medicinal material pest and disease damage after rudiment.

Description

A kind of method of medicinal material Seed inducement germination
Technical field
The present invention relates to the technical field of the technical field of medicinal material plantation, the more particularly to germination of medicinal material Seed inducement.
Background technology
The most of Chinese medicine used at present can be obtained by artificial quick plantation, but still have part medicinal material because of germination The reason such as difficulty, rudiment cycle length, growth conditions harshness, it is impossible to obtained by the method for artificial quick cultivation, thus cause this Part price of medicinal material is remained high, while its wild resource is constantly mined, and both destroys ecological environment, also can substantially cause it wild Raw resource exhaustion.
Wherein more representative medicinal material such as Paris polyphylla and sealwort, Paris polyphylla are liliaceous plant, and medicinal part is Yunnan The effect of Paris polyphylla or the dry rhizome of paris polyphylla, there is clearing heat and detoxicating a, swelling and pain relieving, and cool liver is calmed the frightened, available for treating Furunculosis carbuncle swells, abscess of throat, snake bite and insect sting, the pain of injury caused by falling and tumbling, convulsion etc., in some common medicines, as Yunnan Baiyao aerosol, Red antivenom tablet, SHENNONG PAIN RELIEVING PATCH, Diamond blunt, Gongxuening capsule etc. defended is important component, but its rudiment cycle is extremely long, Growth rate is slower after rudiment, and active component accumulation is slower, causes that its price is high, and wild resource is constantly reduced.
Sealwort also known as even polygonatum sibiricum Redoute, yellow chicken dish, claw ginseng, pipe dish etc. are tired weary available for treatment deficiency of spleen-QI and stomach-QI, body The symptoms such as power, deficiency of stomach-Yin, dry deficiency of food, deficiency syndrome of the lung cough caused by dryness, asthenia of essence and blood, poliosis etc., Nei Rexiao be thirsty, modern medicine study It was found that it has anti-aging, decompression antibacterial, strengthen immunity and other effects.Sealwort can use root by root-like stock or seminal propagation During shape stem breeding, its is slow-growing, and bud ratio is low, and loss is big, during using seminal propagation, rudiment cycle length, if taking tissue The modes such as culture, planting cost and plantation difficulty increase can be caused again so that drug price is higher.
The content of the invention
It is an object of the invention to propose that one kind can effectively shorten the medicinal material seed germination cycle, promote germination, significantly Artificial growth medical material quanlity is improved, the method for the medicinal material Seed inducement germination of medicinal material pest and disease damage after reduction rudiment.
Technical scheme is as follows:
A kind of method of medicinal material Seed inducement germination, comprises the following steps:
(1)The medicinal material seed of no disease and pests harm, removing kind skin are selected, and the seed after the kind skin of removing and decortication is collected respectively;
(2)The kind skin of gained is soaked in syrup, and irradiates 24 ~ 72h under ultraviolet light, thereafter by the kind shelling after immersion It is broken, make the 6 ~ 12h that is soaked in water, obtained liquid facilitates agent for first;
(3)Seed after decortication is soaked in second and facilitates 3 ~ 7d in agent, is soaked seed, described second facilitates agent as in brassin Ester, amine fresh fat, compound sodium nitrophenolate, ammonium nitrate, potassium nitrate, the mixed solution of 6-benzyl aminopurine;
(4)The seed soaking is facilitated in agent from second and taken out, then is soaked in first and facilitates 1 ~ 3d in agent, obtains pre-seeding;
(5)It will be sowed after the pre-seeding low temperature drying.
First facilitates agent and facilitates agent with second and be respectively provided with the effect of promoting medicinal material germination in the above method, and seed exists Order during immersion is quite crucial, and it must first be soaked in second and facilitate in agent, then is soaked in first and facilitates agent, if both Order is switched, then the effect of not having sprout-induction, the step(2)In ultraviolet irradiation can not only effectively facilitate in kind of skin The conversion of material, while the disease resistance of medicinal material after seed germination can be strengthened so that the medicinal material planted by the method for the present invention can It is not required to carry out other reagent desinsections again.
Preferably:The step(2)Described in the concentration of syrup be 0.1 ~ 0.7wt%.
It is also preferred that:The step(2)Described in the wavelength of ultraviolet be 280 ~ 430nm, intensity is 0.3 ~ 1.2W/ m²。
It is also preferred that:The step(2)In the dosage that is used to soak the water of the kind skin ground be per 100g broken kind of skin Add 500 ~ 2000g water.
It is also preferred that:Described second concentration for facilitating brassin lactones in agent is 100 ~ 300mg/L, and described second urges The concentration for sending out compound sodium nitrophenolate in agent is 10 ~ 20mg/L.
It is also preferred that:Described second facilitates 30 ~ 70mg/L of concentration of amine fresh fat in agent.
It is also preferred that:Described second concentration for facilitating ammonium nitrate in agent is 10 ~ 15mg/L, the concentration of potassium nitrate be 1 ~ 10mg/L。
It is also preferred that:Described second concentration for facilitating 6-benzyl aminopurine in agent is 1 ~ 10mg/L.
The above-mentioned preferred embodiment that each component concentration in agent is facilitated on second can further shorten sprouting for medicinal material seed The bud cycle.
It is also preferred that:The step(5)Described in drying temperature be 10 ~ 20 DEG C.
Blowing apparatus can be increased in the drying course to shorten drying time.
It is also preferred that:The medicinal material seed is selected from any of Paris polyphylla or sealwort seed.
The present invention possesses following beneficial effect:
(1)The rudiment cycle of medicinal material seed is substantially reduced, when 50 ~ 75% rudiment on the basis of the former rudiment cycle, can be reduced Between;
(2)The resistance against diseases of medicinal material after rudiment is significantly improved, the prevention and control of plant diseases, pest control can be carried out without pass through any other reagent again;
(3)The content of active component in medicinal material after rudiment can be improved;
(4)Process of the present invention is simple, easily controllable, can carry out large-scale application;
(5)The present invention carries out medicinal material by seed and bred, and has great economic benefit.
Embodiment
The present invention is described in further detail by the following examples, but this should not be interpreted as into the scope of the present invention It is only limitted to following example.In the case where not departing from above method thought of the present invention, according to ordinary skill knowledge and The various replacements or change that customary means is made, should be included in the scope of the present invention.
Embodiment 1
(1)The Paris polyphylla seed of no disease and pests harm, removing kind skin are selected, and the seed after the kind skin of removing and decortication is collected respectively;
(2)The kind skin of gained is soaked in the syrup that concentration is 0.1wt%;It is 280nm in wavelength, intensity is 0.3W/m2 purple 24h is irradiated under outside line, kind of a skin is leached and is milled, carries out immersion 6h using water thereafter, the dosage of water is to add per broken kind of skin of 100g Enter 500g water, obtain first and facilitate agent;
(3)Seed after decortication is soaked in second and facilitates 3d in agent, is soaked seed, described second facilitate agent for solvent be water, Solute and solute concentration are brassin lactones 200mg/L, amine fresh fat 30mg/L, compound sodium nitrophenolate 10mg/L, ammonium nitrate 10mg/L, nitre Sour potassium 1mg/L, 6-benzyl aminopurine 1mg/L solution;
(4)The seed soaking is facilitated in agent from second and taken out, then is soaked in first and facilitates 1d in agent, obtains pre-seeding;
(5)Sowed after the pre-seeding is dried at 10 DEG C;
It is during sowing that undressed basic condition is close(Individual Quality is close, harvest time is identical, condition of storage is identical, same Sample no disease and pests harm)Paris polyphylla seed, and only carry out second facilitate agent immersion Paris polyphylla seed respectively as the first control sample with Second control sample is sowed simultaneously, in identical soil, and under conditions of illumination, temperature, humidity are essentially identical, test As a result understand, the average sprouting time of the first control sample is 30 months, and the average sprouting time of the second control sample is 20 Month, and be 9 months by the average sprouting time of Paris polyphylla seed made from above-mentioned steps, after the seed germination after the present embodiment processing The growth rate of plant is also significantly faster than that the first control sample and the second control sample, does not occur disease pest in whole growth cycle Harmful, the content of active material is higher than the content 1.2% of active material in undressed rhizoma paris rhizome in its rhizome.
Embodiment 2
(1)The Paris polyphylla seed of no disease and pests harm, removing kind skin are selected, and the seed after the kind skin of removing and decortication is collected respectively;
(2)The kind skin of gained is soaked in the syrup that concentration is 0.7wt%;It is 430nm in wavelength, intensity is 0.3W/m2 purple 72h is irradiated under outside line, kind of a skin is leached and is milled, carries out immersion 12h using water thereafter, the dosage of water is to add per broken kind of skin of 100g Enter 2000g water, obtain first and facilitate agent;
(3)Seed after decortication is soaked in second and facilitates 7d in agent, is soaked seed, described second facilitate agent for solvent be water, Solute and solute concentration are brassin lactones 300mg/L, amine fresh fat 70mg/L, compound sodium nitrophenolate 20mg/L, ammonium nitrate 15mg/L, nitre Sour potassium 10mg/L, 6-benzyl aminopurine 10mg/L solution;
(4)The seed soaking is facilitated in agent from second and taken out, then is soaked in first and facilitates 3d in agent, obtains pre-seeding;
(5)Sowed after the pre-seeding is dried at 30 DEG C;
It is during sowing that undressed basic condition is close(Individual Quality is close, harvest time is identical, condition of storage is identical, same Sample no disease and pests harm)Paris polyphylla seed, and only carry out second facilitate agent immersion Paris polyphylla seed respectively as the first control sample with Second control sample is sowed simultaneously, in identical soil, and under conditions of illumination, temperature, humidity are essentially identical, test As a result understand, the average sprouting time of the first control sample is 24 months, and the average sprouting time of the second control sample is 17 Month, and be 6 months by the average sprouting time of Paris polyphylla seed made from above-mentioned steps, after the seed germination after the present embodiment processing The growth rate of plant is also significantly faster than that the first control sample and the second control sample, does not occur disease pest in whole growth cycle Harmful, the content of active material is higher than the content 2% of active material in undressed rhizoma paris rhizome in its rhizome.
Embodiment 3
(1)The Paris polyphylla seed of no disease and pests harm, removing kind skin are selected, and the seed after the kind skin of removing and decortication is collected respectively;
(2)The kind skin of gained is soaked in the syrup that concentration is 0.5wt%;It is 350nm in wavelength, intensity is 1.0W/m2 purple 48h is irradiated under outside line, kind of a skin is leached and is milled, carries out immersion 8h using water thereafter, the dosage of water is to add per broken kind of skin of 100g Enter 1000g water, obtain first and facilitate agent;
(3)Seed after decortication is soaked in second and facilitates 5d in agent, is soaked seed, described second facilitate agent for solvent be water, Solute and solute concentration are brassin lactones 100mg/L, amine fresh fat 50mg/L, compound sodium nitrophenolate 15mg/L, ammonium nitrate 12mg/L, nitre Sour potassium 7mg/L, 6-benzyl aminopurine 5mg/L solution;
(4)The seed soaking is facilitated in agent from second and taken out, then is soaked in first and facilitates 2d in agent, obtains pre-seeding;
(5)Sowed after the pre-seeding is dried at 25 DEG C;
It is during sowing that undressed basic condition is close(Individual Quality is close, harvest time is identical, condition of storage is identical, same Sample no disease and pests harm)Paris polyphylla seed, and only carry out second facilitate agent immersion Paris polyphylla seed respectively as the first control sample with Second control sample is sowed simultaneously, in identical soil, and under conditions of illumination, temperature, humidity are essentially identical, test As a result understand, the average sprouting time of the first control sample is 28 months, and the average sprouting time of the second control sample is 18 Month, and be 6 months by the average sprouting time of Paris polyphylla seed made from above-mentioned steps, after the seed germination after the present embodiment processing The growth rate of plant is also significantly faster than that the first control sample and the second control sample, does not occur disease pest in whole growth cycle Harmful, the content of active material is higher than the content 1.5% of active material in undressed rhizoma paris rhizome in its rhizome.
Embodiment 4
(1)The sealwort seed of no disease and pests harm, removing kind skin are selected, and the seed after the kind skin of removing and decortication is collected respectively;
(2)The kind skin of gained is soaked in the syrup that concentration is 0.1wt%;It is 280nm in wavelength, intensity is 0.3W/m2 purple 24h is irradiated under outside line, kind of a skin is leached and is milled, carries out immersion 6h using water thereafter, the dosage of water is to add per broken kind of skin of 100g Enter 500g water, obtain first and facilitate agent;
(3)Seed after decortication is soaked in second and facilitates 3d in agent, is soaked seed, described second facilitate agent for solvent be water, Solute and solute concentration are brassin lactones 200mg/L, amine fresh fat 30mg/L, compound sodium nitrophenolate 10mg/L, ammonium nitrate 10mg/L, nitre Sour potassium 1mg/L, 6-benzyl aminopurine 1mg/L solution;
(4)The seed soaking is facilitated in agent from second and taken out, then is soaked in first and facilitates 1d in agent, obtains pre-seeding;
(5)Sowed after the pre-seeding is dried at 10 DEG C;
It is during sowing that undressed basic condition is close(Individual Quality is close, harvest time is identical, condition of storage is identical, same Sample no disease and pests harm)Sealwort seed, and only carry out second facilitate agent immersion sealwort seed respectively as the first control sample with Second control sample is sowed simultaneously, in identical soil, and under conditions of illumination, temperature, humidity are essentially identical, test As a result understand, the average sprouting time of the first control sample is 18 months, and the average sprouting time of the second control sample is 16 Month, and be 4 months by the average sprouting time of sealwort seed made from above-mentioned steps, after the seed germination after the present embodiment processing The growth rate of plant is also significantly faster than that the first control sample and the second control sample, does not occur disease pest in whole growth cycle Harmful, the content of active material is higher than the content 1.6% of active material in undressed sealwort rhizome in its rhizome.
Embodiment 5
(1)The sealwort seed of no disease and pests harm, removing kind skin are selected, and the seed after the kind skin of removing and decortication is collected respectively;
(2)The kind skin of gained is soaked in the syrup that concentration is 0.7wt%;It is 430nm in wavelength, intensity is 1.2W/m2 purple 72h is irradiated under outside line, kind of a skin is leached and is milled, carries out immersion 12h using water thereafter, the dosage of water is to add per broken kind of skin of 100g Enter 2000g water, obtain first and facilitate agent;
(3)Seed after decortication is soaked in second and facilitates 7d in agent, is soaked seed, described second facilitate agent for solvent be water, Solute and solute concentration are brassin lactones 150mg/L, amine fresh fat 70mg/L, compound sodium nitrophenolate 20mg/L, ammonium nitrate 15mg/L, nitre Sour potassium 10mg/L, 6-benzyl aminopurine 10mg/L solution;
(4)The seed soaking is facilitated in agent from second and taken out, then is soaked in first and facilitates 3d in agent, obtains pre-seeding;
(5)Sowed after the pre-seeding is dried at 30 DEG C;
It is during sowing that undressed basic condition is close(Individual Quality is close, harvest time is identical, condition of storage is identical, same Sample no disease and pests harm)Sealwort seed, and only carry out second facilitate agent immersion sealwort seed respectively as the first control sample with Second control sample is sowed simultaneously, in identical soil, the illumination of each sample, temperature, humidity in sowing and growth course It is essentially identical etc. environmental condition, and under conditions of illumination, temperature, humidity are essentially identical, the average rudiment of the first control sample Time is 15 months, and the average sprouting time of the second control sample is 11 months, and passes through sealwort seed made from above-mentioned steps Average sprouting time is 3 months, and the growth rate of plant is also significantly faster than that first pair after the seed germination after the present embodiment processing Product and the second control sample in the same old way, do not occur pest and disease damage in whole growth cycle, the content of active material is not higher than in its rhizome The content 2.1% of active material in sealwort rhizome through processing.
Embodiment 6
(1)The sealwort seed of no disease and pests harm, removing kind skin are selected, and the seed after the kind skin of removing and decortication is collected respectively;
(2)The kind skin of gained is soaked in the syrup that concentration is 0.5wt%;It is 380nm in wavelength, intensity is 1.0W/m2 purple 48h is irradiated under outside line, kind of a skin is leached and is milled, carries out immersion 10h using water thereafter, the dosage of water is to add per broken kind of skin of 100g Enter 1500g water, obtain first and facilitate agent;
(3)Seed after decortication is soaked in second and facilitates 6d in agent, is soaked seed, described second facilitate agent for solvent be water, Solute and solute concentration are brassin lactones 250mg/L, amine fresh fat 30mg/L, compound sodium nitrophenolate 10mg/L, ammonium nitrate 10mg/L, nitre Sour potassium 7mg/L, 6-benzyl aminopurine 6mg/L solution;
(4)The seed soaking is facilitated in agent from second and taken out, then is soaked in first and facilitates 2d in agent, obtains pre-seeding;
(5)Sowed after the pre-seeding is dried at 25 DEG C;
It is during sowing that undressed basic condition is close(Individual Quality is close, harvest time is identical, condition of storage is identical, same Sample no disease and pests harm)Sealwort seed, and only carry out second facilitate agent immersion sealwort seed respectively as the first control sample with Second control sample is sowed simultaneously, in identical soil, the illumination of each sample, temperature, humidity in sowing and growth course It is essentially identical etc. environmental condition, and under conditions of illumination, temperature, humidity are essentially identical, the average rudiment of the first control sample Time is 12 months, and the average sprouting time of the second control sample is 8 months, and is put down by sealwort seed made from above-mentioned steps Equal sprouting time is 3 months, and the growth rate of plant is also significantly faster than that the first control after the seed germination after the present embodiment processing Sample and the second control sample, do not occur pest and disease damage in whole growth cycle, in its rhizome the content of active material be higher than without The content 3% of active material in the sealwort rhizome of processing.
Although reference be made herein to invention has been described for explanatory embodiment of the invention, and above-described embodiment is only this hair Bright preferable embodiment, embodiments of the present invention are simultaneously not restricted to the described embodiments, it should be appreciated that people in the art Member can be designed that a lot of other modifications and embodiment, and these modifications and embodiment will fall in principle disclosed in the present application Within scope and spirit.

Claims (10)

  1. A kind of 1. method of medicinal material Seed inducement germination, it is characterised in that:Comprise the following steps:
    (1)The medicinal material seed of no disease and pests harm, removing kind skin are selected, and the seed after the kind skin of removing and decortication is collected respectively;
    (2)The kind skin of gained is soaked in syrup, and irradiates 24 ~ 72h under ultraviolet light, thereafter by the kind shelling after immersion It is broken, make the 6 ~ 12h that is soaked in water, obtained liquid facilitates agent for first;
    (3)Seed after decortication is soaked in second and facilitates 3 ~ 7d in agent, is soaked seed, described second facilitates agent as in brassin Ester, amine fresh fat, compound sodium nitrophenolate, ammonium nitrate, potassium nitrate, the mixed solution of 6-benzyl aminopurine;
    (4)The seed soaking is facilitated in agent from second and taken out, then is soaked in first and facilitates 1 ~ 3d in agent, obtains pre-seeding;
    (5)It will be sowed after the pre-seeding low temperature drying.
  2. 2. the method for medicinal material Seed inducement germination according to claim 1, it is characterised in that:The step(2)Described in The concentration of syrup is 0.1 ~ 0.7wt%.
  3. 3. the method for medicinal material Seed inducement germination according to claim 1, it is characterised in that:The step(2)Described in The wavelength of ultraviolet is 280 ~ 430nm, and intensity is 0.3 ~ 1.2W/m2.
  4. 4. the method for medicinal material Seed inducement germination according to claim 1, it is characterised in that:The step(2)In be used for The dosage for soaking the water of the kind skin ground is to add 500 ~ 2000g water per broken kind of skin of 100g.
  5. 5. the method for medicinal material Seed inducement germination according to claim 1, it is characterised in that:Described second facilitates rue in agent The concentration of tongue element lactone is 100 ~ 300mg/L, and the described second concentration for facilitating compound sodium nitrophenolate in agent is 10 ~ 20mg/L.
  6. 6. the method for medicinal material Seed inducement germination according to claim 1, it is characterised in that:Described second facilitates amine in agent 30 ~ 70mg/L of concentration of fresh fat.
  7. 7. the method for medicinal material Seed inducement germination according to claim 1, it is characterised in that:Described second facilitates nitre in agent The concentration of sour ammonium is 10 ~ 15mg/L, the concentration of potassium nitrate is 1 ~ 10mg/L.
  8. 8. the method for medicinal material Seed inducement germination according to claim 1, it is characterised in that:Described second facilitates 6- in agent The concentration of benayl aminopurine is 1 ~ 10mg/L.
  9. 9. the method for medicinal material Seed inducement germination according to claim 1, it is characterised in that:The step(5)Described in Drying temperature is 10 ~ 20 DEG C.
  10. 10. the method for medicinal material Seed inducement germination according to claim 1, it is characterised in that:The medicinal material seed is choosing From any of Paris polyphylla or sealwort seed.
CN201710613915.3A 2017-07-25 2017-07-25 A kind of method of medicinal material Seed inducement germination Pending CN107372591A (en)

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CN108370678A (en) * 2018-01-08 2018-08-07 浙江大学 The method for promoting David's-harp seed to sprout
CN111657068A (en) * 2020-06-17 2020-09-15 劲牌有限公司 Method for breeding epimedium seedlings

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Publication number Priority date Publication date Assignee Title
CN108370678A (en) * 2018-01-08 2018-08-07 浙江大学 The method for promoting David's-harp seed to sprout
CN108370678B (en) * 2018-01-08 2021-01-01 浙江大学 Method for promoting germination of polygonatum cyrtonema seeds
CN111657068A (en) * 2020-06-17 2020-09-15 劲牌有限公司 Method for breeding epimedium seedlings

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Application publication date: 20171124