CN109379921A - A method of accelerating rhizoma polygonati seed and sprouts - Google Patents
A method of accelerating rhizoma polygonati seed and sprouts Download PDFInfo
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- CN109379921A CN109379921A CN201811116426.8A CN201811116426A CN109379921A CN 109379921 A CN109379921 A CN 109379921A CN 201811116426 A CN201811116426 A CN 201811116426A CN 109379921 A CN109379921 A CN 109379921A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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Abstract
The present invention relates to agricultural planting production fields, specifically refer to a kind of method that quickening rhizoma polygonati seed is sprouted, before rhizoma polygonati seed is sprouted and is cultivated, first rhizoma polygonati seed is put into 40 DEG C of warm water and impregnates 16-18h, water level is 1/4 to 1/3 place of seed, it is put into later and carries out processing 1-2d with soak, processing is immediately placed in refrigerating chamber after completing and handles 0.5-1h, storage time, that is, dormancy time of the rhizoma polygonati seed can artificial adjustment, prevent external environment from changing, cause seed germinating time elongated or shorten, misses best plantation opportunity.
Description
Technical field
The present invention relates to agricultural planting production field, in particular to a kind of methods accelerating rhizoma polygonati seed and sprouting.
Background technique
Base plant one of of the rhizoma polygonati as Chinese medicine rhizoma polygonati in pharmacopeia has immune anti-aging, enhancing, study and memory
Reproduction, anti-inflammatory, antibacterial, antiviral and antitumor, hypoglycemic, reducing blood lipid effect.Rhizoma polygonati main product is in Hebei, the Inner Mongol, Shan
The provinces and regions such as Xi Sheng, polygonatum kingianurn main product mainly originate in Guizhou, Hunan, Yunnan etc. in the provinces and regions such as Guizhou, Yunnan, Guangxi, polygonatum cyrtonema
Provinces and regions.In recent years, the gradually understanding of rhizoma polygonati medicinal and health value, the market demand of rhizoma polygonati are increased with people,
Wild rhizoma polygonati is destroyed by a large amount of exploitations, growing environment, and manual inseminating is imperative, the breeding of rhizoma polygonati include sexual propagation and
Vegetative propagation and rhizoma polygonati mainly uses vegetative manner to produce for a long time, cause its offspring to degenerate and its serious, direct shadow
The yield and quality of rhizoma polygonati medicinal material is rung.Using the breeding of rhizoma polygonati true seed, hybrid vigour can be not only played, improves disease resistance
And breeding coefficient, kind of a quality degradation can also be prevented, is the effective way for solving the breeding of rhizoma polygonati clonal virus and preventing kind of sexual involution
Diameter, but it is also fewer for the research of seed sprouting at present.
Application No. is CN201810355357.X to disclose a kind of seed tissue culture mating system of rhizoma polygonati, passes through mature Huang
Smart seed carries out being put into culture in culture medium after ethylene processing, gibberellin processing, sterilization treatment, finally carries out management of sprouting,
Solve the problems, such as that rhizoma polygonati germination percentage is low, planting scale is small, low output, but in terms of for rhizoma polygonati the storage of seeds there is also,
The problems such as storage life is short, mouldy.
Summary of the invention
The present invention is in order to solve the above technical problems, provide a kind of method that quickening rhizoma polygonati seed is sprouted.
It is realized particular by following technical scheme: a method of accelerating rhizoma polygonati seed and sprout, in rhizoma polygonati seed
It sprouts before cultivating, first rhizoma polygonati seed is put into 40 DEG C of warm water and impregnates 16-18h, water level is 1/4 to 1/3 place of seed, later
It is put into and carries out processing 1-2d with soak, processing is immediately placed in refrigerating chamber after completing and handles 0.5-1h, in the soak
The concentration of kinetin and konjaku slurries is respectively 3-18mg/L, and 30-90mg/L, the sprouting major influence factors of rhizoma polygonati seed have outer
Boundary's environment, illumination condition, the in itself absorption to nutriment, before this carried out disinfection to rhizoma polygonati seed, remove damage, mouldy portion
Position, prevents mouldy position to having an impact elsewhere, is then placed in warm water and impregnates, the seed being positive between being in hibernation
The environment of mild and moist is provided, seed cell is promoted to be divided, is germinateed, seed embryo in germination process can absorb nutrients
Matter accelerates the metabolism of seed, however rhizoma polygonati kind skin has the cell of one layer of lignifying, not only will affect seed and carries out respiration,
It also can be reduced the absorption of nutriment, contain water-soluble polysaccharide in konjaku slurries, nutriment can be provided for seed, secondly low
Under temperature refrigeration, konjaku slurries, which can be attached on seed, forms a tunic, which has loose more hollow structures, moreover it is possible to adsorb rubber fruit enzyme
The small-molecule substances such as protein, starch after solution utilize convenient for seed, and furthermore the film also has the function of heat preservation, moisturizing, kind
The extension of sub- dormant period is also related with intraseminal endogenous inhibitor matter, in the presence of endogenous inhibitor matter, can generate inhibition embryo
The hormone of laticiferous cell division reduces germination percentage so as to cause seed dormancy, and the alternating irradiation of infrared light and ultraviolet light can control kind
The generation of endogenous inhibitor matter in son controls neutron absorption nutriment in turn, when carbon dioxide mass fraction is greater than 5%,
Under the action of ultraviolet light, aerobic respiration and anaerobic respiration are carried out, also, anaerobic exhales and is greater than aerobic respiration, seed is in suspend mode
Phase, when carbon dioxide weight fraction is less than 0.34, under the action of infrared light, seed only carries out aerobic respiration, germination, but
It is to cause germination slower since endogenous inhibitor matter content is also increase accordingly at this time, and be in carbon dioxide mass fraction
0.34-5%, under the action of infrared light, seed carries out aerobic respiration and anaerobic respiration, at this time carbon dioxide can stimulate seed into
Row aerobic respiration, also, the content of intraseminal endogenous inhibitor matter is minimum, aerobic respiration are greater than anaerobic respiration, thin in seed
Born of the same parents' division is vigorous, and germination percentage is high.
Further, the concentration of kinetin is 6mg/L in the soak, and the concentration of konjaku slurries is 70mg/L.
Further, it before rhizoma polygonati seed being carried out immersion treatment, first carries out disinfection processing to rhizoma polygonati seed, step is such as
Under: the rhizoma polygonati seed after first selecting first, which is placed in the tri-sodium phosphate aqueous solution that mass fraction is 5%, impregnates 10-30min, clearly
It washes 3-4 times, places into middle immersion 10-20min in the aqueous slkali that mass fraction is 0.45%.Further, rhizoma polygonati seed is sprouted
Culture specifically refers to, and rhizoma polygonati seed is put into the transparent glass culture dish with filter paper, is placed in constant incubator and is trained
It supports, is subsequently moved in chest, wherein constant incubator temperature is 10-22 DEG C, and incubator humidity is 75-85%, and incubation time is
20-45d。
Further, the chest appearance opens to have hole, and the outer surrounding of case has light source to be shining into, and light source is infrared light and ultraviolet
The time that light, infrared light and ultraviolet light individually irradiate is respectively 6h, 8h.
Further, it after being handled in refrigerating chamber, is put into seed soaking agent and impregnates, contain 20g/L's in the seed soaking agent
The rubber fruit extracting solution of polyethylene glycol and 3g/L, after the rubber fruit extracting solution impregnates 12h in warm water by rubber fruit, filtering, powder
It is broken, enzyme is added, then takes 2 parts of crushing rubber fruit to mix extraction 6h with 35 parts of ethyl alcohol and is soaked seed then in 35 DEG C of isothermal holdings
Rhizoma polygonati seed is finally put into seed soaking agent and handles by agent.
Further, the processing specifically includes following steps, and rhizoma polygonati seed and soak are dispensed by weight 1:15
Into retort pouch, in 15 DEG C of immersion 18h, oxygen, the carbon dioxide of 2 times of retort pouch volumes are filled with into retort pouch, wherein dioxy
The mass fraction for changing carbon is 0.34-5%, seals, then retort pouch is placed in super-pressure tank and handles 5min under 120MPa, takes
Out, 4h is impregnated at 25 DEG C, then retort pouch is placed in super-pressure tank and handles 5min under 100MPa, is taken out, is soaked at 35 DEG C
Steep 2h.
Further, described select specifically refers to that stage of ripeness rhizoma polygonati seed will be harvested, and cleaning is dried, and is therefrom chosen lossless
Wound, the seed for not carrying disease pest ovum are spare.
In conclusion the beneficial effects of the present invention are: the first, the germination percentage of the rhizoma polygonati is up to 89%, and yield is high, no public affairs
Harmful, adaptable, the loose porous film of the rear surface of plantation is hardly damaged, and is easy to absorb the element in soil, is reduced chemical fertilizer
Application, secondly, can also prevent insect in soil from destroying plantation epidermis；The second, storage time, that is, suspend mode of the rhizoma polygonati seed
Time can artificial adjustment, prevent external environment from changing, cause seed germinating time elongated or shorten, when missing best plantation
Machine；Third, rhizoma polygonati seed disease resistance are high, compare and vegetative propagation, improve 20%, breeding coefficient is high, is suitable for extensive
Plantation.
Specific embodiment
Specific embodiments of the present invention will be described in further detail below, but the invention is not limited to these realities
Mode is applied, it is claimed to still fall within the claims in the present invention for any improvement or replacement on the present embodiment essence spirit
Range.
Embodiment 1
Take out refrigerating chamber in rhizoma polygonati seed, be put into seed soaking agent and impregnate, the polyethylene glycol in seed soaking agent containing 20g/L and
The rubber fruit extracting solution of 3g/L after rubber fruit extracting solution impregnates 12h in warm water by rubber fruit, is filtered, is crushed, enzyme is added, then takes 2
The crushing rubber fruit of part mixes extraction 6h with 35 parts of ethyl alcohol and obtains seed soaking agent then in 35 DEG C of isothermal holdings, finally by rhizoma polygonati seed
It is put into seed soaking agent and handles, by rhizoma polygonati seed seed soaking agent immersion treatment specifically includes the following steps: by rhizoma polygonati seed and seed soaking
Agent is dispensed by weight 1:15 into retort pouch, in 15 DEG C of immersion 18h, be filled with into retort pouch 2 times of retort pouch volumes oxygen,
Carbon dioxide, wherein the mass fraction of carbon dioxide be 0.34-5%, sealing, then by retort pouch be placed in super-pressure tank in
5min is handled under 120MPa, is taken out, 4h is impregnated at 25 DEG C, then retort pouch is placed in super-pressure tank under 100MPa and is handled
5min takes out, impregnates 2h at 35 DEG C；
Embodiment 2
Take out refrigerating chamber in rhizoma polygonati seed, be put into seed soaking agent and impregnate, the polyethylene glycol in seed soaking agent containing 20g/L and
The rubber fruit extracting solution of 3g/L after rubber fruit extracting solution impregnates 12h in warm water by rubber fruit, is filtered, is crushed, enzyme is added, then takes 2
The crushing rubber fruit of part mixes extraction 6h with 35 parts of ethyl alcohol and obtains seed soaking agent then in 35 DEG C of isothermal holdings, finally by rhizoma polygonati seed
It is put into seed soaking agent and handles, by rhizoma polygonati seed seed soaking agent immersion treatment specifically includes the following steps: by rhizoma polygonati seed and seed soaking
Agent is dispensed by weight 1:15 into retort pouch, in 15 DEG C of immersion 18h, be filled with into retort pouch 2 times of retort pouch volumes oxygen,
Carbon dioxide, wherein the mass fraction of carbon dioxide is 0.30%, sealing, then retort pouch is placed in super-pressure tank in 120MPa
Lower processing 5min takes out, 4h is impregnated at 25 DEG C, then retort pouch is placed in super-pressure tank and handles 5min under 100MPa, taken
Out, 2h is impregnated at 35 DEG C；
Embodiment 3
Take out refrigerating chamber in rhizoma polygonati seed, be put into seed soaking agent and impregnate, the polyethylene glycol in seed soaking agent containing 20g/L and
The rubber fruit extracting solution of 3g/L after rubber fruit extracting solution impregnates 12h in warm water by rubber fruit, is filtered, is crushed, enzyme is added, then takes 2
The crushing rubber fruit of part mixes extraction 6h with 35 parts of ethyl alcohol and obtains seed soaking agent then in 35 DEG C of isothermal holdings, finally by rhizoma polygonati seed
It is put into seed soaking agent and handles, by rhizoma polygonati seed seed soaking agent immersion treatment specifically includes the following steps: by rhizoma polygonati seed and seed soaking
Agent is dispensed by weight 1:15 into retort pouch, in 15 DEG C of immersion 18h, be filled with into retort pouch 2 times of retort pouch volumes oxygen,
Carbon dioxide, wherein the mass fraction of carbon dioxide is 4.2%, sealing, then retort pouch is placed in super-pressure tank in 120MPa
Lower processing 5min takes out, 4h is impregnated at 25 DEG C, then retort pouch is placed in super-pressure tank and handles 5min under 100MPa, taken
Out, 2h is impregnated at 35 DEG C；
Embodiment 4
Take out refrigerating chamber in rhizoma polygonati seed, be put into seed soaking agent and impregnate, the polyethylene glycol in seed soaking agent containing 20g/L and
The rubber fruit extracting solution of 3g/L after rubber fruit extracting solution impregnates 12h in warm water by rubber fruit, is filtered, is crushed, enzyme is added, then takes 2
The crushing rubber fruit of part mixes extraction 6h with 35 parts of ethyl alcohol and obtains seed soaking agent then in 35 DEG C of isothermal holdings, finally by rhizoma polygonati seed
It is put into seed soaking agent and handles, by rhizoma polygonati seed seed soaking agent immersion treatment specifically includes the following steps: by rhizoma polygonati seed and seed soaking
Agent is dispensed by weight 1:15 into retort pouch, in 15 DEG C of immersion 18h, be filled with into retort pouch 2 times of retort pouch volumes oxygen,
Carbon dioxide, wherein the mass fraction of carbon dioxide is 12%, sealing, then retort pouch is placed in super-pressure tank under 120MPa
5min is handled, takes out, 4h is impregnated at 25 DEG C, then retort pouch is placed in super-pressure tank and handles 5min under 100MPa, is taken out,
2h is impregnated at 35 DEG C；
Embodiment 5
Rhizoma polygonati seed is put into seed soaking agent and is impregnated, the rubber fruit of polyethylene glycol and 3g/L in seed soaking agent containing 20g/L mentions
It after taking liquid, rubber fruit extracting solution to impregnate 12h in warm water by rubber fruit, filters, crushes, enzyme is added, then takes 2 parts of crushing rubber fruit
Extraction 6h is mixed with 35 parts of ethyl alcohol and obtains seed soaking agent then in 35 DEG C of isothermal holdings, and finally rhizoma polygonati seed is put into seed soaking agent
Processing, by rhizoma polygonati seed seed soaking agent immersion treatment specifically includes the following steps: by rhizoma polygonati seed and seed soaking agent by weight 1:
15 packing, in 15 DEG C of immersion 18h, oxygen, the carbon dioxide of 2 times of retort pouch volumes are filled with into retort pouch into retort pouch,
The mass fraction of middle carbon dioxide is 0.30%, sealing, then retort pouch is placed in super-pressure tank under 120MPa and is handled
5min takes out, 4h is impregnated at 25 DEG C, then retort pouch is placed in super-pressure tank and handles 5min under 100MPa, takes out, in 35
2h is impregnated at DEG C；
Embodiment 6
The rhizoma polygonati seed in refrigerating chamber is taken out, the rubber fruit extracting solution of the polyethylene glycol containing 20g/L and 3g/L in seed soaking agent,
It after rubber fruit extracting solution impregnates 12h in warm water by rubber fruit, filters, crushes, enzyme is added, then takes 2 parts of crushing rubber fruit and 35
Part ethyl alcohol mixing extraction 6h obtains seed soaking agent then in 35 DEG C of isothermal holdings, and finally rhizoma polygonati seed is put into seed soaking agent
Reason, by rhizoma polygonati seed seed soaking agent immersion treatment specifically includes the following steps: by rhizoma polygonati seed and seed soaking agent by weight 1:15
Packing, in 15 DEG C of immersion 18h, air, the carbon dioxide of 2 times of retort pouch volumes is filled with into retort pouch into retort pouch, wherein
The mass fraction of carbon dioxide is 0.30%, sealing, then retort pouch is placed in super-pressure tank and handles 5min under 120MPa, is taken
Out, 4h is impregnated at 25 DEG C, then retort pouch is placed in super-pressure tank and handles 5min under 100MPa, is taken out, is soaked at 35 DEG C
Steep 2h；
Embodiment 7
The rhizoma polygonati seed in refrigerating chamber is taken out, the rubber fruit extracting solution of the polyethylene glycol containing 20g/L and 3g/L in seed soaking agent,
It after rubber fruit extracting solution impregnates 12h in warm water by rubber fruit, filters, crushes, then take 2 parts of crushing rubber fruit mixed with 35 parts of ethyl alcohol
It closes extraction 6h and obtains seed soaking agent then in 35 DEG C of isothermal holdings, finally rhizoma polygonati seed is put into seed soaking agent and is handled, by rhizoma polygonati
Seed seed soaking agent immersion treatment by weight 1:15 specifically includes the following steps: dispensing rhizoma polygonati seed and seed soaking agent to boiling
In bag, in 15 DEG C of immersion 18h, oxygen, the carbon dioxide of 2 times of retort pouch volumes are filled with into retort pouch, wherein carbon dioxide
Mass fraction is 0.30%, sealing, then retort pouch is placed in super-pressure tank and handles 5min under 120MPa, is taken out, in 25 DEG C
Lower immersion 4h, then retort pouch is placed in super-pressure tank and handles 5min under 100MPa, it takes out, impregnates 2h at 35 DEG C.
Claims (8)
1. a kind of method accelerating rhizoma polygonati seed and sprouting, which is characterized in that before rhizoma polygonati seed is sprouted and cultivated, first by rhizoma polygonati kind
Son, which is put into 40 DEG C of warm water, impregnates 16-18h, and water level is 1/4 to 1/3 place of seed, is put into carries out processing 1-2d with soak later,
Processing is immediately placed in refrigerating chamber after completing and handles 0.5-1h, and the concentration of kinetin and konjaku slurries is distinguished in the soak
For 3-18mg/L, 30-90mg/L.
2. accelerating the method that rhizoma polygonati seed is sprouted as described in claim 1, which is characterized in that kinetin in the soak
Concentration is 6mg/L, and the concentration of konjaku slurries is 70mg/L.
3. accelerating the method that rhizoma polygonati seed is sprouted as described in claim 1, which is characterized in that carry out rhizoma polygonati seed at immersion
Before reason, first carry out disinfection processing to rhizoma polygonati seed, steps are as follows: the rhizoma polygonati seed after first selecting first is placed in mass fraction
To impregnate 10-30min in 5% tri-sodium phosphate aqueous solution, cleans 3-4 times, place into the aqueous slkali that mass fraction is 0.45%
In middle immersion 10-20min.
4. accelerating the method that rhizoma polygonati seed is sprouted as described in claim 1, which is characterized in that it is specific that rhizoma polygonati seed sprouts culture
Refer to, rhizoma polygonati seed is put into the transparent glass culture dish with filter paper, is placed in constant incubator and is cultivated, with moving back
Enter in chest, wherein constant incubator temperature is 10-22 DEG C, and incubator humidity is 75-85%, incubation time 20-45d.
5. chest as claimed in claim 4, which is characterized in that the chest appearance opens to have hole, and the outer surrounding of case has light source photograph
Enter, light source is infrared light and ultraviolet light, and the time that infrared light and ultraviolet light individually irradiate is respectively 6h, 8h.
6. accelerating the method that rhizoma polygonati seed is sprouted as described in claim 1, which is characterized in that after being handled in refrigerating chamber,
It is put into seed soaking agent and impregnates, the rubber fruit extracting solution of the polyethylene glycol and 3g/L in the seed soaking agent containing 20g/L, the rubber fruit mentions
It after taking liquid to impregnate 12h in warm water by rubber fruit, filters, crushes, enzyme is added, then take 2 parts of crushing rubber fruit and 35 parts of ethyl alcohol
Mixing extraction 6h obtains seed soaking agent then in 35 DEG C of isothermal holdings, and finally rhizoma polygonati seed is put into seed soaking agent and is handled.
7. being handled as claimed in claim 6 with seed soaking agent rhizoma polygonati seed, which is characterized in that it is described processing specifically include with
Lower step dispenses rhizoma polygonati seed and seed soaking agent into retort pouch by weight 1:15, in 15 DEG C of immersion 18h, into retort pouch
It is filled with oxygen, the carbon dioxide of 2 times of retort pouch volumes, wherein the mass fraction of carbon dioxide is 0.34-5%, is sealed, then will steam
It boils bag and is placed in super-pressure tank and handle 5min under 120MPa, take out, 4h is impregnated at 25 DEG C, then retort pouch is placed in super-pressure
5min is handled under 100MPa in tank, takes out, impregnates 2h at 35 DEG C.
8. the method for promoting rhizoma polygonati seed to sprout as claimed in claim 3, which is characterized in that described select specifically refers to adopt
Stage of ripeness rhizoma polygonati seed is received, cleaning is dried, and it is spare therefrom to choose seed that is not damaged, not carrying disease pest ovum.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110521325A (en) * | 2019-09-04 | 2019-12-03 | 昆明坤地农业开发有限公司 | A kind of method of polygonatum kingianurn seed fast seedling growing |
| CN110734891A (en) * | 2019-12-07 | 2020-01-31 | 顾霆 | Process flow of cell continuous culture tank |
| CN114946497A (en) * | 2022-04-27 | 2022-08-30 | 江西普正制药股份有限公司 | Seedling raising method for improving germination rate and regularity of callicarpa nudiflora |
| RU2817568C1 (en) * | 2023-11-10 | 2024-04-16 | Федеральное государственное бюджетное учреждение науки Пермский федеральный исследовательский центр Уральского отделения Российской академии наук (ПФИЦ УрО РАН) | Method for pre-sowing treatment of seeds of grain crops by laser radiation |
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| RU2817568C1 (en) * | 2023-11-10 | 2024-04-16 | Федеральное государственное бюджетное учреждение науки Пермский федеральный исследовательский центр Уральского отделения Российской академии наук (ПФИЦ УрО РАН) | Method for pre-sowing treatment of seeds of grain crops by laser radiation |
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