CN108029278B - Method for improving germination rate of picea rasmae seeds by using gibberellin - Google Patents
Method for improving germination rate of picea rasmae seeds by using gibberellin Download PDFInfo
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- CN108029278B CN108029278B CN201711297858.9A CN201711297858A CN108029278B CN 108029278 B CN108029278 B CN 108029278B CN 201711297858 A CN201711297858 A CN 201711297858A CN 108029278 B CN108029278 B CN 108029278B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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Abstract
The invention provides a method for improving germination rate of picea rasmae seeds by using gibberellin, which comprises the following steps: 1) soaking sterile picea asperata seeds in 0.3-0.8 mg/L gibberellin solution for 20-28 h to obtain pretreated seeds; 2) mixing the pretreated seeds obtained in the step 1) with pretreated river sand according to a volume ratio of 1: and (2) mixing the components in proportion to obtain a germination accelerating system, and stacking the germination accelerating system at 10-15 ℃ for accelerating germination for 7-10 days to obtain the leucocyte seeds. The method can effectively break seed dormancy, improve the germination rate of the seeds, and ensure that the emergence rate of the spruce on sand is more than 90 percent.
Description
Technical Field
The invention belongs to the technical field of cultivation of picea rasmae, and particularly relates to a method for improving seed germination rate of picea rasmae by using gibberellin.
Background
The spruce in sand belongs to evergreen arbor, and has a height of 30m and a diameter at breast height of about 60 cm; the crown of the picea asperata is gray, blue-green, and the bark is scaly, is light-loving, and is relatively shade-tolerant, cold-tolerant, drought-tolerant and barren-tolerant. Spruce in sandy land is a rare and precious tree species, and is produced in eastern part of muddy dakesha in inner Mongolia of China. The side root system of the spruce in the sand is developed, the root length is 3 times of that of the trunk, the root system is spread and interwoven, the root is staggered, and the fine sand which is scattered can be gathered together, so that the spruce has special effects on wind prevention and sand fixation.
At present, the seed treatment of the spruce seedling in the sand field usually adopts a laminated germination method after the seed disinfection, and due to the seed dormancy, the emergence rate after the sowing is low, the emergence is not regular, and the seedling protection rate in the later seedling raising process is low.
Disclosure of Invention
In view of the above, the invention aims to provide a method for improving the germination rate of picea rasmae seeds by using gibberellin, so that the seedling protection rate of later-stage seedling raising can be improved.
In order to achieve the above object, the present invention provides the following technical solutions: a method for improving germination rate of picea asperata seeds by using gibberellin comprises the following steps: 1) soaking sterile picea asperata seeds for 20-28 h by using 0.3-0.8 mg/L gibberellin solution to obtain pretreated seeds; 2) mixing the pretreated seeds obtained in the step 1) with pretreated river sand according to a volume ratio of 1: and (2) mixing the materials according to the proportion to obtain a germination accelerating system, and stacking the germination accelerating system at 10-15 ℃ for accelerating germination for 7-10 days to obtain the white exposed seeds.
Preferably, the concentration of the gibberellin solution in the step 1) is 0.4-0.6 mg/L.
Preferably, the seed soaking time in the step 1) is 22-26 h.
Preferably, the river sand pretreated in the step 2) is river sand which is sequentially washed, disinfected by carbendazim, aired and soaked by gibberellin solution.
Preferably, the volume ratio of the pretreated seeds to the pretreated river sand is 1: (3-4).
Preferably, the mass concentration of the carbendazim is 0.45-0.55%.
Preferably, the concentration of the gibberellin solution soaked in the river sand is 0.4-0.6 mg/L.
Preferably, the sterile spruce sand seed in step 1) is prepared by KMnO of spruce sand seed4Soaking the solution for 1-3 h to obtain the product.
Preferably, the KMnO4The mass concentration of the solution is 0.4-0.6%.
Preferably, in the germination accelerating process in the step 2), the germination accelerating system is turned over once every 20-28 hours. The invention has the beneficial effects that: according to the method, the gibberellin solution is adopted to treat sterile picea asperata seeds, exogenous gibberellin is utilized to accelerate hydrolysis of starch in endosperm of the seeds, seed germination is promoted, seed dormancy can be effectively broken through, the germination rate of the seeds is improved, and meanwhile, germination is accelerated by a sand storage method, and seedlings emerge orderly.
Furthermore, the river sand soaked by the gibberellin solution and the pretreated seeds are mixed and stacked for accelerating germination, so that the germination rate of the spruce seeds in the sand is over 90 percent.
Detailed Description
The invention provides a method for improving germination rate of picea rasmae seeds by using gibberellin, which comprises the following steps: 1) soaking sterile picea asperata seeds for 20-28 h by using 0.3-0.8 mg/L gibberellin solution to obtain pretreated seeds; 2) mixing the pretreated seeds obtained in the step 1) with pretreated river sand according to a volume ratio of 1: and (2) mixing the materials according to the proportion to obtain a germination accelerating system, and stacking the germination accelerating system at 10-15 ℃ for accelerating germination for 7-10 days to obtain the white exposed seeds.
Before the sterile spruce sand field seed is pretreated, the cleaning of the spruce sand field seed is preferably carried out; the cleaning is preferably carried out by adopting a water floating method, specifically, standby picea asperata seeds are soaked in water for 15-25 min, blighted seeds floating on the water surface are removed, and the plump seeds are fished out for disinfection to obtain sterile picea asperata seeds. The sterile spruce sand seed in the present invention is preferably prepared with spruce sand seed through KMnO4Soaking the solution for 1-3 h to obtain the product; the KMnO4The mass concentration of the solution is preferably 0.4-0.6%, more preferably 0.5%; the soaking time is preferably 2 hours. The KMnO4After soaking in the solution, preferably draining to obtain sterile spruce seeds.
In the invention, sterile picea asperata seeds are soaked for 20-28 h by using 0.3-0.8 mg/L gibberellin solution to obtain pretreated seeds. The concentration of the gibberellin solution is preferably 0.4-0.6 mg/L, and more preferably 0.5 mg/L; the seed soaking time is preferably 22-26 hours, and more preferably 24 hours. In the invention, the gibberellin can stimulate Ca on the cell membrane of the seed through a series of biological effects+Transport protein activity, promoting cellular absorption of Ca by consuming ATP+And Ca+But also can induce the synthesis of alpha-amylase of the seeds, thereby accelerating the hydrolysis of starch in the endosperm of the seeds and promoting the germination of the seeds. Therefore, the gibberellin has the physiological effects of relieving seed dormancy and stimulating the growth of the embryo with the seed dormancy completed, and can obviously improve the germination rate and the germination vigor of the seeds.
After the seed soaking is finished, the seed soaked is preferably fished out and drained.
After the pretreatment seeds are obtained, the volume ratio of the pretreatment seeds to the pretreatment river sand is 1: and (2) mixing the components in the ratio of (2) to (5) to obtain a germination accelerating system.
The pretreated river sand is river sand which is soaked by a gibberellin solution and is washed by water, disinfected by carbendazim and dried in the air. In the invention, the mass concentration of the carbendazim is preferably 0.45-0.55%, and more preferably 0.5%; the method for sterilizing carbendazim in the present invention is not particularly limited, and may be a method that is conventional in the art. In the invention, the river sand is preferably dried after the carbendazim is disinfected; in the specific implementation process of soaking river sand with the gibberellin solution, the gibberellin solution is preferably sprayed into the aired river sand, and the concentration of the sprayed gibberellin solution is preferably 0.3-0.8 mg/L, more preferably 0.4-0.6 mg/L, and most preferably 0.5 mg/L. The spraying amount of the gibberellin solution is not specially limited, and the gibberellin solution can soak river sand; specifically, the pretreated river sand obtained after spraying the gibberellin solution in the examples is in a state of being held by hand to form a mass and being loosened after being loosened by one pine. The invention aims to provide a high-concentration exogenous gibberellin environment for spruce seeds in sand to improve the dormancy breaking rate of the seeds by adopting gibberellin to pretreat river sand.
The volume ratio of the pretreated seeds to the pretreated river sand is preferably 1 (3-4), and more preferably 1: 3.
After the germination accelerating system is obtained, the germination accelerating system is stacked at the temperature of 10-15 ℃ for accelerating germination for 7-10 days, and the white exposed seeds are obtained. In the invention, the germination accelerating temperature is preferably 9-14 ℃; the germination accelerating time is preferably 8-9 days; turning over the germination accelerating system in the germination accelerating process, wherein the turning time interval is preferably 20-28 h, and more preferably 22-26 h; most preferably 24 hours. The germination accelerating system is preferably filled into woven bags for stacking.
The germination accelerating method has the advantages of preserving heat and moisture, rapidly relieving seed dormancy, saving seeds, effectively improving the germination rate of seedlings, shortening the seedling emergence period, ensuring the uniformity of seedling emergence, enhancing the quality of seedlings and further reducing the seedling raising cost and the occurrence of diseases. Experimental results show that the spruce seeds processed by the method are sowed in sandy soil and covered by fine sand, the emergence time is concentrated in 8-14 days after sowing, the emergence rate is over 90 percent, the seedling height is about 2.3cm, and the variation coefficient is 3.8 percent. The germinated seeds obtained by the method disclosed by the invention preferably have the white exposure of more than 50%.
The method for improving germination rate of spruce sand seeds by using gibberellin provided by the present invention is described in detail with reference to the following examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Setting up an experimental group and a control group
Experimental groups were treated as follows:
(1) seed cleaning: soaking the stand-by Picea salsa seeds in water for 20min by using a water float method, removing blighted seeds floating on the water surface, and fishing out full seeds.
(2) Seed disinfection: soaking the seeds of (1) in 0.5% KMnO4Taking out the solution after 2h and draining.
(3) Soaking the seeds with gibberellin: soaking the seeds treated in the step (2) in 0.5mg/L gibberellin solution for 24h, taking out and draining water.
(4) Accelerating germination of seeds: the sand storage method is adopted. Firstly, cleaning river sand with water, disinfecting with 0.5% carbendazim, spraying 0.5mg/L gibberellin solution after drying, wherein the humidity is suitable for being held by hands to form clusters and being scattered by one pine, and then mixing the treated spruce seeds of sand with the river sand according to the volume ratio of 1:3, placing the mixture into a woven bag, stacking the woven bag at 10 ℃, turning the mixture once every 26 hours in the germination accelerating process, and sowing the mixture after more than 50 percent of the spruce seeds are exposed for 10 days. The rate of emergence of the seeds was calculated to be 91% after sowing.
The contrast group uses clear water to replace gibberellin solution to soak the spruce seeds, uses clear water to replace gibberellin solution to soak river sand, and has the same other steps as the experimental group, and the emergence rate of the contrast group is 64%.
The emergence rate of the experimental group seeds is improved by 42 percent compared with that of the control group seeds.
Example 2
Setting up an experimental group and a control group
Experimental groups were treated as follows:
(3) seed cleaning: soaking the stand-by Picea salsa seeds in water for 18min by using a water float method, removing blighted seeds floating on the water surface, and fishing out full seeds.
(4) Seed disinfection: soaking the seeds of (1) in 0.5% KMnO4Taking out the solution after 2h and draining.
(3) Soaking the seeds with gibberellin: soaking the seeds treated in the step (2) in 0.5mg/L gibberellin solution for 24h, taking out and draining water.
(4) Accelerating germination of seeds: the sand storage method is adopted. Firstly, cleaning river sand with water, disinfecting with 0.5% carbendazim, spraying 0.5mg/L gibberellin solution after drying, wherein the humidity is suitable for being held by hands to form clusters and being scattered by one pine, and then mixing the treated spruce seeds of sand with the river sand according to the volume ratio of 1:3, placing the mixture into a woven bag, stacking the woven bag at 15 ℃, turning the mixture once every 24 hours in the germination accelerating process, and sowing the mixture after more than 50 percent of the spruce seeds are exposed for 7 days. The rate of emergence of the seeds after sowing was calculated to be 90%.
The contrast group uses clear water to replace gibberellin solution to soak the spruce seeds, uses clear water to replace gibberellin solution to soak river sand, and the other steps are the same as those of the experimental group, and the emergence rate of the contrast group is 46%.
The seed emergence rate of the experimental group is improved by 44 percent compared with that of the control group.
Example 3
Setting up an experimental group and a control group
Experimental groups were treated as follows:
(1) seed cleaning: soaking the stand-by Picea salsa seeds in water for 20min by using a water float method, removing blighted seeds floating on the water surface, and fishing out full seeds.
(2) Seed disinfection: soaking the seeds of (1) in 0.5% KMnO4Taking out the solution after 2h and draining.
(3) Soaking the seeds with gibberellin: soaking the seeds treated in the step (2) in 0.5mg/L gibberellin solution for 24h, taking out and draining water.
(4) Accelerating germination of seeds: the sand storage method is adopted. Firstly, cleaning river sand with water, disinfecting with 0.5% carbendazim, spraying 0.5mg/L gibberellin solution after drying, wherein the humidity is suitable for being held by hands to form clusters and being scattered by one pine, and then mixing the treated spruce seeds of sand with the river sand according to the volume ratio of 1:3, filling the mixture into a woven bag, stacking the woven bag at 12 ℃, turning the woven bag once every 25 hours in the germination accelerating process, and sowing the spruce seeds after more than 50 percent of the spruce seeds are exposed for 8 days. The rate of emergence of the seeds after sowing was calculated to be 94%.
The contrast group uses clear water to replace gibberellin solution to soak the spruce seeds, uses clear water to replace gibberellin solution to soak river sand, and has the same other steps as the experimental group, and the emergence rate of the contrast group is 72%.
The seed emergence rate of the experimental group is improved by 30 percent compared with that of the control group.
Example 4
(1) Seed cleaning: soaking the stand-by Picea salsa seeds in water for 20min by using a water float method, removing blighted seeds floating on the water surface, and fishing out full seeds.
(2) Seed disinfection: soaking the seeds of (1) in 0.5% KMnO4Taking out the solution after 2h and draining.
(3) Soaking the seeds with gibberellin: soaking the seeds treated in the step (2) in 0.5mg/L gibberellin solution for 24h, taking out and draining water.
(4) Accelerating germination of seeds: the sand storage method is adopted. Firstly, cleaning river sand with water, disinfecting with 0.5% carbendazim, spraying clear water after drying, wherein the humidity is suitable for being held into a ball and being scattered by one pine, and then mixing the treated spruce seeds on the sand with the river sand according to the volume ratio of 1:3, placing the mixture into a woven bag, stacking the woven bag at 15 ℃, turning the mixture once every 24 hours in the germination accelerating process, and sowing the mixture after more than 50 percent of the spruce seeds are exposed for 8 days. The rate of emergence of the seeds after sowing was calculated to be 75%.
Example 5
(1) Seed cleaning: soaking the stand-by Picea salsa seeds in water for 20min by using a water float method, removing blighted seeds floating on the water surface, and fishing out full seeds.
(2) Seed disinfection: soaking the seeds of (1) in 0.5% KMnO4Taking out the solution after 2h and draining.
(3) Soaking the seeds with gibberellin: soaking the seeds treated in the step (2) in 0.5mg/L gibberellin solution for 24h, taking out and draining water.
(4) Accelerating germination of seeds: the sand storage method is adopted. Firstly, cleaning river sand with water, disinfecting with 0.5% carbendazim, spraying clear water after drying, wherein the humidity is suitable for being held into a ball and being scattered by one pine, and then mixing the treated spruce seeds on the sand with the river sand according to the volume ratio of 1:3, filling the mixture into a woven bag, stacking the woven bag at 12 ℃, turning the woven bag once every 25 hours in the germination accelerating process, and sowing the spruce seeds after more than 50 percent of the spruce seeds are exposed for 9 days. The rate of emergence of the seeds after sowing was calculated to be 59%.
Example 6
(1) Seed cleaning: soaking the stand-by Picea salsa seeds in water for 20min by using a water float method, removing blighted seeds floating on the water surface, and fishing out full seeds.
(2) Seed disinfection: soaking the seeds of (1) in 0.5% KMnO4Taking out the solution after 2h and draining.
(3) Soaking the seeds with gibberellin: soaking the seeds treated in the step (2) in 0.5mg/L gibberellin solution for 22h, taking out and draining water.
(4) Accelerating germination of seeds: the sand storage method is adopted. Firstly, cleaning river sand with water, disinfecting with 0.5% carbendazim, spraying clear water after drying, wherein the humidity is suitable for being held into a ball and being scattered by one pine, and then mixing the treated spruce seeds on the sand with the river sand according to the volume ratio of 1:3, placing the mixture into a woven bag, stacking the woven bag at 10 ℃, turning the mixture once every 26 hours in the germination accelerating process, and sowing the mixture after more than 50 percent of the spruce seeds are exposed for 10 days. The rate of emergence of the seeds after sowing was calculated to be 83%.
The embodiment shows that the method can promote the germination of the seeds, effectively break the dormancy of the seeds, improve the emergence rate of the seeds by over 90 percent, ensure consistent germination time and tidy emergence.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (1)
1. A method for improving germination rate of picea asperata seeds by using gibberellin comprises the following steps:
(1) seed cleaning: soaking the stand-by Picea salsa seeds in water for 20min by using a water float method, removing blighted seeds floating on the water surface, and fishing out full seeds;
(2) seed disinfection: soaking the seeds of (1) in 0.5% KMnO4Taking out the solution after 2h and draining;
(3) soaking the seeds with gibberellin: soaking the seeds treated in the step (2) in 0.5mg/L gibberellin solution for 24h, taking out and draining;
(4) accelerating germination of seeds: cleaning river sand with water, disinfecting with 0.5% carbendazim, air drying, spraying 0.5mg/L gibberellin solution, and spraying the solution with humidity suitable for being held by hand to form clusters and scattered by one pine, wherein the volume ratio of the treated spruce seeds to the river sand is 1:3, filling the mixture into a woven bag, stacking the woven bag at 12 ℃, turning the woven bag once every 25 hours in the germination accelerating process, and sowing the spruce seeds after more than 50 percent of the spruce seeds are exposed for 8 days.
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| CN109005738A (en) * | 2018-08-16 | 2018-12-18 | 贵州大学 | A method of promoting Chimonocalamus utilis seed germination rate |
| CN109005744B (en) * | 2018-09-15 | 2020-10-30 | 烟台市林业科学研究所 | Method for improving germination rate of callicarpa bodinieri seeds by using gibberellin |
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| CN106171798A (en) * | 2016-08-26 | 2016-12-07 | 甘肃省治沙研究所 | A kind of Picea Mongolica land for growing field crops, Arid Desert Area seed seedling-raising method |
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| 不同化学物质处理对沙地云杉种子活力的影响;高春智 等;《不同化学物质处理对沙地云杉种子活力的影响》;森林工程;20130131;第32卷(第1期);第10-15页 * |
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