CN109511313A - A kind of presprouting of seeds agent of polygonatum cyrtonema and its germination accelerating method - Google Patents
A kind of presprouting of seeds agent of polygonatum cyrtonema and its germination accelerating method Download PDFInfo
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- CN109511313A CN109511313A CN201910034248.2A CN201910034248A CN109511313A CN 109511313 A CN109511313 A CN 109511313A CN 201910034248 A CN201910034248 A CN 201910034248A CN 109511313 A CN109511313 A CN 109511313A
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- seed
- polygonatum cyrtonema
- presprouting
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- seeds agent
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Abstract
Presprouting of seeds agent and its germination accelerating method the invention discloses a kind of polygonatum cyrtonema, the composition of each raw material of the germinator by weight percentage are as follows: lignoenzyme 1%-3%, cellulase 0.5%-1%, carbendazim 1%-10%, remaining is distilled water.The present invention breaks polygonatum cyrtonema seed dormancy by the presprouting of seeds reagent prepared, and can greatly shorten the polygonatum cyrtonema seed seedling-raising period, reduces production cost, can solve the problems, such as that polygonatum cyrtonema seedling is insufficient.
Description
Technical field
The present invention relates to a kind of Liliaceae HUANGJING ZANYU CAPSULE, the presprouting of seeds agent of polygonatum cyrtonema and its germination accelerating method.
Background technique
Polygonatum cyrtonema is Liliaceae Polygonatum perennial root herbaceous plant, and it is of equal value to collect medicinal, edible, ornamental and beauty
Value is.Polygonatum cyrtonema meat is fine and smooth, and rhizomes has the function such as strengthening spleen and invigorating qi, moistening lung kidney-nourishing, nourishing Yin and promoting production of body fluid similar to ginger
Effect, is considered as rare Chinese medicine by middle medical circles since ancient times, there is the good reputation of " king of vim and vigour giving young employees remedial-courses in general knowledge and vocational skills ".States Pharmacopoeia specifications are as medicinal
HUANGJING ZANYU CAPSULE be mainly distributed across the rhizoma polygonati of the northern area of China, be distributed in it is more on the south the polygonatum kingianurn and the Changjiang river in the Yunnan-Guizhou Plateau
Three kinds of rhizoma polygonati of flower.
Currently, the polygonatum cyrtonema to circulate in the market is all the wild resource of excavation mostly, farmer's transplanting mostly uses wild
Rhizoma polygonati carries out stem tuber breeding, and breeding coefficient is low, quality is very different, while causing the destruction of native germplasm；Using seed
Nursery then can solve problem above, but due to polygonatum cyrtonema seed there are suspend mode the phenomenon that, use self seed to need 3 years
Ability germinating to seedling, and germinating to seedling rate is low.
Chinese patent CN103858769A discloses " a kind of quick breeding technology method of rhizoma polygonati ", and this method passes through hormone
Repeatedly processing, which breaks rhizoma polygonati seed dormancy and makees material using seed, carries out in-vitro inducing culture callus, trains by differentiation
In the stages such as feeding and root induction, obtain vegetative propagation rhizoma polygonati seedling.This method vernalization just needs to handle in two times using two kinds of hormones,
Used hormone is expensive, higher cost, complex steps；From Seed inducement callus to squamous subculture, differentiation culture and
Root induction, seedling time need to extend again three to four months, and higher cost is unsuitable for industrialization promotion.
Chinese patent CN201710416032.3 is disclosed " a kind of mating system of polygonatum cyrtonema seed ", uses ground
The sand storages polygonatum cyrtonema seed such as cellar, straw, while using indoor change temperature-accelerated germination.But the facilities such as cellar do not have generality, and
Be not suitable for a wide range of promote.And this method was also needed by the more very long alternating temperature stage, growing-seedling period is long, is unsuitable for intensive
Using.
Chinese patent CN201710220080.5 discloses " mating system of rhizoma polygonati seed ", impregnated using refrigeration, warm water,
Brassinosteroid and the method for gibberellin integrated treatment handle rhizoma polygonati seed, though germination percentage is preferably, hormone, refrigeration cost compared with
Height, process is excessively complicated, is not suitable for large-scale application.
Chinese patent CN201510817849.2 discloses " a kind of method of polygonatum cyrtonema seed seedling-raising ", raw using ABT
The mixed liquor of root powder, indolebutyric acid and carbendazim aqueous solution mixes sand storage polygonatum cyrtonema seed in wet fine sand, the seed after sand storage
It sows with fine sand in the substrate soil mixed in vinyl house by sawdust, the dregs of a decoction, pine bark, waters sooner or later daily together.
But its period is up to 5~7 months, is also unable to reach the purpose of fast and convenient Pregermination and seedling breeding.
With the increase of the market demand, breaks polygonatum cyrtonema seed dormancy, the technical barrier for overcoming seed seedling-raising, provides surely
The problem of fixed seedling is supplied, and current polygonatum cyrtonema industry development urgent need to resolve is become.
Seed anatomy to polygonatum cyrtonema the result shows that: embryo point of visible cotyledon and plumule former base in fruit maturation
Change, but radicle and plumular axis differentiation are unobvious；For kind skin by the cell composition of one layer of part of wall lignifying, endosperm structure is fine and close, influences
The rate of water absorption of seed.Therefore, the present invention focuses on the germination percentage that polygonatum cyrtonema seed is promoted in terms of these, utilizes preparation
Vernalization reagent can play the role of promoting seed rate of water absorption, reduce germination inhicbitor, and then improve germination percentage in a short time.
Summary of the invention
To avoid the problems of above-mentioned prior art, the invention proposes a kind of presprouting of seeds agent of polygonatum cyrtonema and
Its germination accelerating method, it is intended to solve the problems, such as that the germination percentage encountered during polygonatum cyrtonema seminal propagation is too low, realize and improve breeding
Coefficient, the purpose for shortening growing-seedling period and save the cost.
To realize goal of the invention, the present invention adopts the following technical scheme:
The present invention discloses a kind of presprouting of seeds agent of polygonatum cyrtonema, the composition of each raw material by weight percentage first
Are as follows: lignoenzyme 1%-3%, cellulase 0.5%-1%, carbendazim 1%-10%, remaining is distilled water.
The present invention further discloses the methods for carrying out vernalization to polygonatum cyrtonema seed using above-mentioned presprouting of seeds agent, special
Point is: by polygonatum cyrtonema fruit in valve bag after fermentation process, removing pericarp and pulp, takes out polygonatum cyrtonema seed；It will
Polygonatum cyrtonema seed is impregnated to break seed dormancy, so by liquor potassic permanganate soaking disinfection, then through the presprouting of seeds agent
Seed and sterilizing river sand are mixed afterwards, carry out sand storage so that seed germination, obtains seed bud.Specifically comprise the following steps:
(1) pre-treatment of seed
Polygonatum cyrtonema fruit is fitted into valve bag and is sealed, room temperature heap fermentation 10-15 days is washed by rubbing with the hands and removes pericarp and fruit
Meat cleans seed, dries well sifted, obtain clean, full polygonatum cyrtonema seed；Processing can accelerate to send out pulp sufficiently in this way
Ferment is more convenient for separating seed and pulp, relatively it is naturally soft it is rotten about shift to an earlier date half a month, raising seed shatter treatment effeciency.
By polygonatum cyrtonema seed mass concentration be 0.1%-0.3% liquor potassic permanganate in soaking disinfection 20-
During which 40min is kept stirring, then take out and rinsed 2~3 times with clear water；Stirring can be such that seed and liquor potassic permanganate fills
Tap touching achievees the effect that sufficiently to sterilize, and the processing of potassium permanganate can make later period presprouting of seeds agent more preferably play a role.
(2) preparation of presprouting of seeds agent
According to the weight percent of each raw material of presprouting of seeds agent, each raw material is mixed, matches to obtain presprouting of seeds agent；The seed
Germinator is ready-to-use, can quickly and easily break seed dormancy by the processing of presprouting of seeds agent.
(3) vernalization condition is controlled, seed dormancy is broken
It will be immersed in presprouting of seeds agent through step (1) treated polygonatum cyrtonema seed, controlled at 35-45 DEG C, magnetic
Power stirs 3-5 hours, to break seed dormancy；Presprouting of seeds agent of the invention promotes kind using lignoenzyme and cellulase
Shell shake solution, sand storage of the collaboration carbendazim in the seed afterripening stage act on, accelerate seed water swelling, greatly promote germination.
(4) Sand culture is with stratification
It is 1:3~5 by seed sand volume ratio, is mixed using the sterilizing river sand and polygonatum cyrtonema seed that humidity is 20-40%,
Sand storage 28 ± 2 days under the conditions of 25 ± 2 DEG C can make Seed sprouting, obtain seed bud.Suitable humidity and ventilation environment may insure
Moisture needed for germination and air environment, while avoiding seed decay and mildewing.
The beneficial effects of the present invention are embodied in:
Presprouting of seeds agent of the invention, the conventional reagent being easy to get using simplicity, can effectively break the seed of polygonatum cyrtonema
Suspend mode shortened to the germinating time of polygonatum cyrtonema seed one month from 1 year, and percentage of seedgermination reaches 92.7%-96.0%,
Reduce production cost, solves the problems, such as that polygonatum cyrtonema seedling is insufficient.
Specific embodiment
The preferred embodiment of the present invention is described in detail with reference to embodiments.It should be appreciated that being preferably implemented
Example is only for illustrating the present invention, rather than limiting the scope of protection of the present invention.
Polygonatum cyrtonema fruit used in following embodiments was acquired from Jinzhai County, Anhui Province, late September to late October, to more
It can be harvested after flower rhizoma polygonati fruit maturation.
Embodiment 1
(1) pre-treatment of seed
Polygonatum cyrtonema fruit is fitted into valve bag, it is room temperature heap fermentation 15 days, soft rotten to most fruits, it can rub with the hands
Wash away pericarp and pulp, flowing water rinses seed until surface is without pulp, dries well sifted, obtains clean, full spending more Huang
Smart seed is put into the container sterilized in advance；Mature qualified polygonatum cyrtonema thousand grain weigth should be greater than 40g.
Polygonatum cyrtonema seed is slowly poured into the liquor potassic permanganate that mass concentration is 0.1%, to reach adequate disinfection
Effect, the half of liquor potassic permanganate volume is not to be exceeded in seed volume, during which soaking disinfection 40min does not stop using glass
Stick stirring, comes into full contact with seed and liquor potassic permanganate and achievees the effect that sufficiently to sterilize.It then takes out and is rinsed with clear water.
(2) preparation of presprouting of seeds agent
According to lignoenzyme 1%, cellulase 0.5%, carbendazim 5%, the weight percent that remaining is distilled water, match
Presprouting of seeds agent, it is ready-to-use.
(3) vernalization condition is controlled, seed dormancy is broken
It will be immersed in presprouting of seeds agent through step (1) treated polygonatum cyrtonema seed, and controlled at 40 DEG C, use magnetic
Power blender does not stop to stir at low speed, and is kept for 3 hours, to break seed dormancy；
(4) Sand culture is with stratification
The thin river sand for taking about seed three times volume is sterilized 3 hours using 130 DEG C of oven, completion subject to sterilization, sand
When temperature is down to room temperature, the polygonatum cyrtonema seed for completing sterilizing and vernalization processing is added, adds appropriate amounts of sterilized water and stirs evenly,
Sand humidity is set to reach 30%, wet sand sand storage one month under the conditions of 25 DEG C, seed is sprouted, and seed bud is formed, random to take out
100 seeds, count germination percentage in triplicate.
Embodiment 2
It is handled by the identical method of embodiment 1, difference is only that the weight percent of each raw material of presprouting of seeds agent are as follows:
Lignoenzyme 1%, cellulase 1%, carbendazim 5%, remaining be distilled water.
Embodiment 3
It is handled by the identical method of embodiment 1, difference is only that the weight percent of each raw material of presprouting of seeds agent are as follows:
Lignoenzyme 3%, cellulase 0.5%, carbendazim 5%, remaining be distilled water.
Embodiment 4
It is handled by the identical method of embodiment 1, difference is only that the weight percent of each raw material of presprouting of seeds agent are as follows:
Lignoenzyme 3%, cellulase 1%, carbendazim 5%, remaining be distilled water.
Embodiment 4
It is handled by the identical method of embodiment 1, difference is only that in step (2), presprouting of seeds agent is changed to identical
The water of volume, as a comparison.
Through counting, from embodiment 1 to embodiment 5, percentage of seedgermination is respectively 92.7%, 94.3%, 96.0%, 93.3%
With 14.7%.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (3)
1. a kind of presprouting of seeds agent of polygonatum cyrtonema, which is characterized in that the composition of each raw material by weight percentage are as follows:
Lignoenzyme 1%-3%, cellulase 0.5%-1%, carbendazim 1%-10%, remaining is distilled water.
2. a kind of method for carrying out vernalization to polygonatum cyrtonema seed using presprouting of seeds agent described in claim 1, feature exist
In:
By polygonatum cyrtonema fruit in valve bag after fermentation process, pericarp and pulp are removed, takes out polygonatum cyrtonema seed；It will spend more
Rhizoma polygonati seed is impregnated by liquor potassic permanganate soaking disinfection, then through the presprouting of seeds agent to break seed dormancy, then will
Seed and sterilizing river sand mix, and carry out sand storage so that seed germination, obtains seed bud.
3. according to the method described in claim 2, it is characterized in that, specifically comprising the following steps:
(1) pre-treatment of seed
Polygonatum cyrtonema fruit is fitted into valve bag and is sealed, room temperature heap fermentation 10-15 days is washed by rubbing with the hands and removes pericarp and pulp, washes
Net seed, dries well sifted, obtains clean, full polygonatum cyrtonema seed；
By polygonatum cyrtonema seed mass concentration be 0.1%-0.3% liquor potassic permanganate in soaking disinfection 20-40min, the phase
Between keep stirring, then take out and with clear water rinse 2~3 times；
(2) preparation of presprouting of seeds agent
According to the weight percent of each raw material of presprouting of seeds agent, each raw material is mixed, matches to obtain presprouting of seeds agent；
(3) vernalization condition is controlled, seed dormancy is broken
It will be immersed in presprouting of seeds agent through step (1) treated polygonatum cyrtonema seed, controlled at 35-45 DEG C, magnetic force is stirred
It mixes 3-5 hours, to break seed dormancy；
(4) Sand culture is with stratification
It is 1:3-5 by seed sand volume ratio, is mixed using the sterilizing river sand and polygonatum cyrtonema seed that humidity is 20-40%, 25 ± 2
Sand storage 28 ± 2 days under the conditions of DEG C can make Seed sprouting, obtain seed bud.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910034248.2A CN109511313A (en) | 2019-01-15 | 2019-01-15 | A kind of presprouting of seeds agent of polygonatum cyrtonema and its germination accelerating method |
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| CN201910034248.2A CN109511313A (en) | 2019-01-15 | 2019-01-15 | A kind of presprouting of seeds agent of polygonatum cyrtonema and its germination accelerating method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110521325A (en) * | 2019-09-04 | 2019-12-03 | 昆明坤地农业开发有限公司 | A kind of method of polygonatum kingianurn seed fast seedling growing |
| CN110537467A (en) * | 2019-09-25 | 2019-12-06 | 贵州百灵企业集团制药股份有限公司 | polygonatum kingianum seed greenhouse seedling growing method |
| CN111316786A (en) * | 2020-04-26 | 2020-06-23 | 浙江省林业科学研究院 | Polygonatum cyrtonema seed germination accelerating and seedling cultivation method |
| CN111837501A (en) * | 2020-06-19 | 2020-10-30 | 重庆市药物种植研究所 | Method for promoting germination of polygonatum cyrtonema seeds |
| CN114830867A (en) * | 2022-04-26 | 2022-08-02 | 云南省农业科学院药用植物研究所 | Method for improving germination rate of polygonatum kingianum seeds |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110521325A (en) * | 2019-09-04 | 2019-12-03 | 昆明坤地农业开发有限公司 | A kind of method of polygonatum kingianurn seed fast seedling growing |
| CN110537467A (en) * | 2019-09-25 | 2019-12-06 | 贵州百灵企业集团制药股份有限公司 | polygonatum kingianum seed greenhouse seedling growing method |
| CN111316786A (en) * | 2020-04-26 | 2020-06-23 | 浙江省林业科学研究院 | Polygonatum cyrtonema seed germination accelerating and seedling cultivation method |
| CN111837501A (en) * | 2020-06-19 | 2020-10-30 | 重庆市药物种植研究所 | Method for promoting germination of polygonatum cyrtonema seeds |
| CN111837501B (en) * | 2020-06-19 | 2022-04-22 | 重庆市药物种植研究所 | Method for promoting germination of polygonatum cyrtonema seeds |
| CN114830867A (en) * | 2022-04-26 | 2022-08-02 | 云南省农业科学院药用植物研究所 | Method for improving germination rate of polygonatum kingianum seeds |
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