CN109197458A - A kind of breeding method of rhizoma polygonati seedling - Google Patents
A kind of breeding method of rhizoma polygonati seedling Download PDFInfo
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- CN109197458A CN109197458A CN201811081455.5A CN201811081455A CN109197458A CN 109197458 A CN109197458 A CN 109197458A CN 201811081455 A CN201811081455 A CN 201811081455A CN 109197458 A CN109197458 A CN 109197458A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Pest Control & Pesticides (AREA)
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- Pretreatment Of Seeds And Plants (AREA)
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Abstract
The invention belongs to agricultural technology fields, especially a kind of breeding method of rhizoma polygonati seedling, specifically includes the following steps: (1) seed collection and storage, (2) seminal propagation, the preparation of (3) seedling medium, the cultivation of (4) seedling, (5) are transplanted.The present invention is handled during the storage of seeds using additive, and the deterioration of seed can be delayed, and seed is made to be able to maintain preferable quality；Using nutrient solution, ultraviolet lamp processing during seminal propagation, it can quickly break the suspend mode of seed, promote its germination.A kind of breeding method of rhizoma polygonati seedling provided by the invention, germination percentage, planting percent are high, and growth cycle is short.
Description
Technical field
The invention belongs to planting technology field, especially a kind of breeding method of rhizoma polygonati seedling.
Background technique
Rhizoma polygonati also known as polygonatum sibiricum Redoute, yellow chicken dish, pen tube dish, claw ginseng, tendrilleaf solomonseal rhizome, achickenclaw ginseng, are the more sward sheets of Liliaceae
Plant.The rhizome of rhizoma polygonati is edible medicinal position, containing ingredients such as steroid saponin, Siberian solomonseal rhizome polysaccharide, anthraquinone, lignanoid, microelements,
Have the effects that tonifying spleen, moistening lung and producing body fluid, reducing blood lipid, hypoglycemic, anti-oxidant, anti-aging, antiviral, antiatherosclerosis,
It is usually used in treating the symptoms such as pulmonary tuberculosis, ringworm, diabetes, hyperlipidemia, hyperglycemia.
For rhizoma polygonati in artificial cultivation based on Propagation of Rhizomes, this modes of reproduction not only results in the waste of medicinal material itself,
Germplasm degeneration is also resulted in, pest and disease damage increases, and has seriously affected the yield and quality of rhizoma polygonati.It is bred using rhizoma polygonati true seed,
Hybrid vigour can be not only played, disease resistance and breeding coefficient is improved, can also prevent germplasm from degenerating, is that solution rhizoma polygonati is virus-free
The effective way for the degeneration for breeding and preventing germplasm from degenerating；But rhizoma polygonati seed have deep-sleep characteristic, growth cycle compared with
Long, germination percentage and planting percent are lower.Currently, the research about rhizoma polygonati seed propagation seedling is less, these researchs are mostly with sand storage
Mode stores seed, improves its germination percentage with plant growth regulator treatment seed.Rhizoma polygonati seed in storage,
Unsaturated fatty acid in seed can aoxidize to form free radical, cause Lipid peroxidation, cause to damage to seed membranous system,
The deterioration for causing seed has seriously affected the germination rate of rhizoma polygonati seed.Such as the file that number of patent application is 201711315295.1
A kind of breeding method of disclosed rhizoma polygonati seedling, rhizoma polygonati seed is uniformly mixed with wet fine sand and is stored, and the patent is wet thin
Other materials maintenance rhizoma polygonati seed membranous system is not added in sand, seed is easy deterioration, causes its germination percentage lower.
Summary of the invention
In order to solve the above technical problems existing in the prior art, the present invention provides a kind of cultivation sides of rhizoma polygonati seedling
Method is realized particular by following technical scheme:
A kind of breeding method of rhizoma polygonati seedling, specifically includes the following steps:
(1) acquisition and storage of seed: in the rhizoma polygonati fruit of 10-11 month harvesting ripe, sunning to water content is lower than 3%,
Select full, no disease and pests harm, the rhizoma polygonati seed without fission；Wet fine sand is uniformly mixed with additive and is fermented 4-6 days in shady spot
Afterwards, then rhizoma polygonati seed mixing is added thereto to bury；
(2) seminal propagation: step (1) treated seed is taken out, 4%H is used2O2Solution disinfection is then placed in nutrition
3-4h is impregnated in liquid, the ultraviolet light irradiation of 30W is used in soaking process, and the distance between ultraviolet source and seed are 60-70cm；
(3) preparation of seedling medium: 10-15 parts of sandy loams, 4-7 parts of agricultural residues powders, 0.3-0.5 are weighed by weight
Part nutrient solution is uniformly mixed, and is fermented 2-5 days at 23-28 DEG C, up to seedling medium after sterilization treatment；
(4) by step (2) treated seed broadcasting into seedling medium, one layer the cultivation of seedling: is covered after broadcasting sowing again
The seedling medium of 3-4cm thickness, is cultivated in greenhouse, controlled at 23-27 DEG C in cultivating process, humidity 70-
80%, ventilation is kept, light application time is 4-6h/ days, and after germination, extending light application time is 12-15h/ days.
(5) it transplants: can be transplanted after seedling grows to 6-10cm.
Preferably, the additive by weight by 10-16 parts of rhizoma polygonati cauline leafs, 1-2 parts of microelements, 5-6 parts it is sweet
Grass, 3-5 parts of folium cortex eucommiaes are made.
Preferably, the microelement is made of sodium metasilicate, manganese chloride, zinc sulfate, copper sulphate, wherein sodium metasilicate, chlorine
Change manganese, zinc sulfate, copper sulphate mass ratio be 1-2:2-4:1-2:0.2-0.5.
Preferably, the additive the preparation method comprises the following steps: rhizoma polygonati cauline leaf, folium cortex eucommiae are crushed, 5-6 times of water is added and decocts
2-2.5h, is cooled to room temperature, then Radix Glycyrrhizae crushed and is added thereto uniformly mixed, mixture is obtained after standing 8-10h, by mixture
In 50-60 DEG C of drying to water content lower than 10% up to additive.
Preferably, the step (1), wet fine sand, additive, rhizoma polygonati seed mass ratio be 2.4-3.2:0.3-0.5:
1。
Preferably, the nutrient solution is by weight by 0.2-0.3 parts of gibberellin, 1-2 parts of calcium chloride, 0.5-1 parts of sulphur
Sour magnesium, 0.5-1 part cysteine, 1000-1500 parts of distilled water are mixed.
Preferably, the radiation modality of the ultraviolet lamp is primary every 1h irradiation, and each irradiation time is 30min.
The beneficial effects of the present invention are: be added additive during rhizoma polygonati the storage of seeds, additive by rhizoma polygonati cauline leaf,
Microelement, Radix Glycyrrhizae, folium cortex eucommiae are made, and can not only inhibit the pollution of storage bacterium, moreover it is possible to make the peroxide in rhizoma polygonati seed
Change the antioxidases such as hydrogen enzyme, superoxide dismutase, peroxidase and keep higher activity, reduces free radical in storage
Damage to seed membranous system has delayed the deterioration of seed；Use gibberellin, calcium chloride, magnesium sulfate, cysteine, distilled water
Manufactured nutrient solution impregnates seed, and gibberellin is adjustable the hormonal balance of Interior Seed, promotes germination, chlorination
Calcium, magnesium sulfate, cysteine can make the hydrolases such as the amylase of seed kind, cellulase keep higher activity, increase in seed
The soluble sugar content in portion promotes metabolic process, accelerates germination；Ultraviolet light irradiation is used in soaking process, promotes kind
Absorption of the son to nutrient solution, while the antioxidase of Interior Seed also being made to keep higher activity.A kind of Huang provided by the invention
The breeding method of smart seedling, germination percentage, planting percent are high, and germination percentage is up to 94% or more, and survival rate is up to 92% or more；Growth week
Phase is short, and germinating time only needs 16-18 days or so.
Specific embodiment
It is limited below with reference to specific embodiment technical solution of the present invention is further, but claimed
Range is not only limited to made description.
Embodiment 1
A kind of breeding method of rhizoma polygonati seedling, specifically includes the following steps:
(1) acquisition and storage of seed: in the rhizoma polygonati fruit of 10-11 month harvesting ripe, sunning to water content is lower than 3%,
Select full, no disease and pests harm, the rhizoma polygonati seed without fission；Wet fine sand is uniformly mixed after shady spot ferments 4 days with additive,
Rhizoma polygonati seed mixing is added thereto again to bury；
(2) seminal propagation: step (1) treated seed is taken out, 4%H is used2O2Solution disinfection is then placed in nutrition
3h is impregnated in liquid, the ultraviolet light irradiation of 30W is used in soaking process, and the distance between ultraviolet source and seed are 60cm；
(3) preparation of seedling medium: 10 parts of sandy loams, 4 parts of agricultural residues powders, 0.3 portion of nutrient solution are weighed by weight
It is uniformly mixed, ferments 2 days at 28 DEG C, up to seedling medium after sterilization treatment；
(4) by step (2) treated seed broadcasting into seedling medium, one layer the cultivation of seedling: is covered after broadcasting sowing again
The seedling medium of 3-4cm thickness, is cultivated in greenhouse, and controlled at 23 DEG C in cultivating process, humidity 70% is protected
Ventilation is held, light application time is 6h/ days, and after germination, extending light application time is 15h/ days.
(5) it transplants: can be transplanted after seedling grows to 6-10cm.
Preferably, the additive is by weight by 10 parts of rhizoma polygonati cauline leafs, 1 part of microelement, 5 portions of Radix Glycyrrhizaes, 3 parts of Du
Zhong Ye is made.
Preferably, the microelement is made of sodium metasilicate, manganese chloride, zinc sulfate, copper sulphate, wherein sodium metasilicate, chlorine
Change manganese, zinc sulfate, copper sulphate mass ratio be 1:2:1:0.2.
Preferably, the additive the preparation method comprises the following steps: rhizoma polygonati cauline leaf, folium cortex eucommiae are crushed, 5 times of water are added and decoct
2h, is cooled to room temperature, then Radix Glycyrrhizae crushed and is added thereto uniformly mixed, mixture is obtained after standing 8h, by mixture in 50-60
It is DEG C dry to water content lower than 10% up to additive.
Preferably, the step (1), wet fine sand, additive, rhizoma polygonati seed mass ratio be 2.4:0.3:1.
Preferably, the nutrient solution is by weight by 0.2 part of gibberellin, 1 part of calcium chloride, 0.5 part of magnesium sulfate, 0.5
Part cysteine, 1000 parts of distilled water are mixed.
Preferably, the radiation modality of the ultraviolet lamp is primary every 1h irradiation, and each irradiation time is 30min.
Embodiment 2
A kind of breeding method of rhizoma polygonati seedling, specifically includes the following steps:
(1) acquisition and storage of seed: in the rhizoma polygonati fruit of 10-11 month harvesting ripe, sunning to water content is lower than 3%,
Select full, no disease and pests harm, the rhizoma polygonati seed without fission；Wet fine sand is uniformly mixed after shady spot ferments 5 days with additive,
Rhizoma polygonati seed mixing is added thereto again to bury；
(2) seminal propagation: step (1) treated seed is taken out, 4%H is used2O2Solution disinfection is then placed in nutrition
3.5h is impregnated in liquid, the ultraviolet light irradiation of 30W is used in soaking process, and the distance between ultraviolet source and seed are 64cm；
(3) preparation of seedling medium: 12 parts of sandy loams, 6 parts of agricultural residues powders, 0.4 portion of nutrient solution are weighed by weight
It is uniformly mixed, ferments 4 days at 24 DEG C, up to seedling medium after sterilization treatment；
(4) by step (2) treated seed broadcasting into seedling medium, one layer the cultivation of seedling: is covered after broadcasting sowing again
The seedling medium of 3-4cm thickness, is cultivated in greenhouse, and controlled at 25 DEG C in cultivating process, humidity 76% is protected
Ventilation is held, light application time is 5h/ days, and after germination, extending light application time is 14h/ days.
(5) it transplants: can be transplanted after seedling grows to 6-10cm.
Preferably, the additive is by weight by 12 parts of rhizoma polygonati cauline leafs, 1.2 parts of microelements, 5.4 parts of Radix Glycyrrhizaes, 4
Part folium cortex eucommiae is made.
Preferably, the microelement is made of sodium metasilicate, manganese chloride, zinc sulfate, copper sulphate, wherein sodium metasilicate, chlorine
Change manganese, zinc sulfate, copper sulphate mass ratio be 1:3:1:0.4.
Preferably, the additive the preparation method comprises the following steps: rhizoma polygonati cauline leaf, folium cortex eucommiae are crushed, 5.2 times of water are added and decoct
2.3h, is cooled to room temperature, then Radix Glycyrrhizae crushed and is added thereto uniformly mixed, mixture is obtained after standing 9h, by mixture in 50-
60 DEG C of dryings are to water content lower than 10% up to additive.
Preferably, the step (1), wet fine sand, additive, rhizoma polygonati seed mass ratio be 3.0:0.4:1.
Preferably, the nutrient solution by weight by 0.25 part of gibberellin, 1.2 parts of calcium chloride, 0.6 part of magnesium sulfate,
0.6 part of cysteine, 1200 parts of distilled water are mixed.
Preferably, the radiation modality of the ultraviolet lamp is primary every 1h irradiation, and each irradiation time is 30min.
Embodiment 3
A kind of breeding method of rhizoma polygonati seedling, specifically includes the following steps:
(1) acquisition and storage of seed: in the rhizoma polygonati fruit of 10-11 month harvesting ripe, sunning to water content is lower than 3%,
Select full, no disease and pests harm, the rhizoma polygonati seed without fission；Wet fine sand is uniformly mixed after shady spot ferments 6 days with additive,
Rhizoma polygonati seed mixing is added thereto again to bury；
(2) seminal propagation: step (1) treated seed is taken out, 4%H is used2O2Solution disinfection is then placed in nutrition
4h is impregnated in liquid, the ultraviolet light irradiation of 30W is used in soaking process, and the distance between ultraviolet source and seed are 70cm；
(3) preparation of seedling medium: 15 parts of sandy loams, 7 parts of agricultural residues powders, 0.5 portion of nutrient solution are weighed by weight
It is uniformly mixed, ferments 5 days at 23 DEG C, up to seedling medium after sterilization treatment；
(4) by step (2) treated seed broadcasting into seedling medium, one layer the cultivation of seedling: is covered after broadcasting sowing again
The seedling medium of 3-4cm thickness, is cultivated in greenhouse, and controlled at 27 DEG C in cultivating process, humidity 80% is protected
Ventilation is held, light application time is 4h/ days, and after germination, extending light application time is 12h/ days.
(5) it transplants: can be transplanted after seedling grows to 6-10cm.
Preferably, the additive is by weight by 16 parts of rhizoma polygonati cauline leafs, 2 parts of microelements, 6 portions of Radix Glycyrrhizaes, 5 parts of Du
Zhong Ye is made.
Preferably, the microelement is made of sodium metasilicate, manganese chloride, zinc sulfate, copper sulphate, wherein sodium metasilicate, chlorine
Change manganese, zinc sulfate, copper sulphate mass ratio be 2:4:2:0.5.
Preferably, the additive the preparation method comprises the following steps: rhizoma polygonati cauline leaf, folium cortex eucommiae are crushed, 6 times of water are added and decoct
2.5h, is cooled to room temperature, then Radix Glycyrrhizae crushed and is added thereto uniformly mixed, obtains mixture after standing 10h, mixture is existed
50-60 DEG C of drying is to water content lower than 10% up to additive.
Preferably, the step (1), wet fine sand, additive, rhizoma polygonati seed mass ratio be 3.2:0.5:1.
Preferably, the nutrient solution is by weight by 0.3 part of gibberellin, 2 parts of calcium chloride, 1 part of magnesium sulfate, 1 part half
Cystine, 1500 parts of distilled water are mixed.
Preferably, the radiation modality of the ultraviolet lamp is primary every 1h irradiation, and each irradiation time is 30min.
Comparative example 1
Comparative example 1 is the difference from embodiment 1 is that without using additive in storage, other processes are identical.
Comparative example 2
Comparative example 2 is not impregnate using nutrient solution during seminal propagation with the difference for implementing 1, but uses pure water
Same time is impregnated, other processes are identical.
Comparative example 3
Comparative example 3 is the difference from embodiment 1 is that without using ultraviolet light irradiation, other process phases during seminal propagation
Together.
Comparative example 4
The difference of comparative example 4 and implementation 1 is that seminal propagation process does not use nutrient solution to impregnate, without using ultraviolet lamp
Irradiation, but pure water is used to impregnate same time, other processes are identical.
Test example
The developmental state of Statistics Implementation example 1-3 and comparative example 1-4 seed, the results are shown in Table 1
Table 1
As seen from the table, compared with comparative example 1, the germination percentage of embodiment 1-3, survival rate are higher, and what the present invention used adds
Add agent that seed can be made to keep higher germination rate；It is that the time is shorter needed for embodiment 1-3 germination compared with comparative example 2-4, germination
Rate, planting percent are higher, are handled using nutrient solution, ultraviolet lamp seed, seed can be promoted to sprout, and shorten seedling-growing time, mention
High seedling percent.
It is important to point out that, above embodiments and test example are only limitted to do further technical solution of the present invention herein
Elaboration and understanding, should not be understood as it is further to technical solution of the present invention limited, what those skilled in the art made
The innovation and creation of non-protruding essential characteristics and marked improvement still fall within protection category of the invention.
Claims (7)
1. a kind of breeding method of rhizoma polygonati seedling, specifically includes the following steps:
(1) acquisition and storage of seed: in the rhizoma polygonati fruit of 10-11 month harvesting ripe, sunning to water content is lower than 3%, selects
Full, no disease and pests harm, the rhizoma polygonati seed without fission；Wet fine sand is uniformly mixed after shady spot ferments 4-6 days with additive, then
Rhizoma polygonati seed is added thereto mixing to bury；
(2) seminal propagation: step (1) treated seed is taken out, 4%H is used2O2Solution disinfection is then placed in nutrient solution
3-4h is impregnated, the ultraviolet light irradiation of 30W is used in soaking process, the distance between ultraviolet source and seed are 60-70cm；
(3) preparation of seedling medium: 10-15 parts of sandy loams, 4-7 parts of agricultural residues powders, 0.3-0.5 parts of battalion are weighed by weight
Nutrient solution is uniformly mixed, and is fermented 2-5 days at 23-28 DEG C, up to seedling medium after sterilization treatment；
(4) cultivation of seedling: by step (2), treated that seed broadcasting covers one layer of 3-4cm again into seedling medium, after broadcasting sowing
Thick seedling medium, is cultivated in greenhouse, controlled at 23-27 DEG C, humidity 70-80% in cultivating process,
Ventilation is kept, light application time is 4-6h/ days, and after germination, extending light application time is 12-15h/ days.
(5) it transplants: can be transplanted after seedling grows to 6-10cm.
2. the breeding method of rhizoma polygonati seedling as described in claim 1, which is characterized in that the additive by weight by
10-16 parts of rhizoma polygonati cauline leafs, 1-2 parts of microelements, 5-6 portions of Radix Glycyrrhizaes, 3-5 parts of folium cortex eucommiaes are made.
3. the breeding method of rhizoma polygonati seedling as claimed in claim 2, which is characterized in that the microelement by sodium metasilicate,
Manganese chloride, zinc sulfate, copper sulphate composition, wherein sodium metasilicate, manganese chloride, zinc sulfate, copper sulphate mass ratio be 1-2:2-4:1-
2:0.2-0.5。
4. the breeding method of rhizoma polygonati seedling as claimed in claim 2, which is characterized in that the preparation method of the additive
Are as follows: rhizoma polygonati cauline leaf, folium cortex eucommiae are crushed, 5-6 times of water is added and decocts 2-2.5h, is cooled to room temperature, then Radix Glycyrrhizae is crushed, it is added
In be uniformly mixed, obtain mixture after standing 8-10h, mixture dry added to water content lower than 10% to obtain the final product at 50-60 DEG C
Add agent.
5. the breeding method of rhizoma polygonati seedling as described in claim 1, which is characterized in that the step (1), wet fine sand add
Add agent, the mass ratio of rhizoma polygonati seed is 2.4-3.2:0.3-0.5:1.
6. the breeding method of rhizoma polygonati seedling as described in claim 1, which is characterized in that the nutrient solution by weight by
0.2-0.3 parts of gibberellin, 1-2 parts of calcium chloride, 0.5-1 parts of magnesium sulfate, 0.5-1 parts of cysteines, 1000-1500 parts of distilled water are mixed
Conjunction is made.
7. the breeding method of rhizoma polygonati seedling as described in claim 1, which is characterized in that the radiation modality of the ultraviolet lamp is
Primary every 1h irradiation, each irradiation time is 30min.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110073956A (en) * | 2019-03-23 | 2019-08-02 | 河南联源生物科技有限公司 | Greenhouse-based sealwort seed seedling raising method |
| CN110226483A (en) * | 2019-07-24 | 2019-09-13 | 云南省林木种苗工作总站(云南省林木种苗花卉产品质量监督检验站) | A kind of polygonatum kingianurn method for culturing seedlings |
| CN110537467A (en) * | 2019-09-25 | 2019-12-06 | 贵州百灵企业集团制药股份有限公司 | polygonatum kingianum seed greenhouse seedling growing method |
| CN110810198A (en) * | 2019-12-05 | 2020-02-21 | 昆明英武农业科技有限公司 | Germination accelerating substrate for polygonatum kingianum seeds and germination accelerating method thereof |
| CN111316786A (en) * | 2020-04-26 | 2020-06-23 | 浙江省林业科学研究院 | Polygonatum cyrtonema seed germination accelerating and seedling cultivation method |
| CN111557219A (en) * | 2020-06-10 | 2020-08-21 | 湖南省农业环境生态研究所 | Polygonatum cyrtonema seedling growing method |
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