CN107182521A - Magnificent Paris polyphylla high efficiency seedling cultivating method - Google Patents

Magnificent Paris polyphylla high efficiency seedling cultivating method Download PDF

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Publication number
CN107182521A
CN107182521A CN201710496075.7A CN201710496075A CN107182521A CN 107182521 A CN107182521 A CN 107182521A CN 201710496075 A CN201710496075 A CN 201710496075A CN 107182521 A CN107182521 A CN 107182521A
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paris polyphylla
seed
conditions
under
magnificent
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CN201710496075.7A
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皮剑波
皮爱国
向勇祥
伍耀先
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Changsha Wisdom-Sun Bioisystech Co Ltd
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Changsha Wisdom-Sun Bioisystech Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting

Abstract

The invention discloses a kind of method of magnificent Paris polyphylla high efficiency seedling cultivating, this method includes：The step such as seed selection, pretreatment, first time low-temperature preservation, first time ferment treatment, second low-temperature preservation, second ferment treatment, Sha Zang, sowing and nursery maintenance.By the method for culturing seedlings, the cycle time of emerging of magnificent Paris polyphylla was to 220 ~ 270 days, and emergence rate reaches more than 85%, substantially reduces the growing-seedling period of Paris polyphylla, improves the emergence rate of Paris polyphylla, can be that the fast breeding of magnificent Paris polyphylla seedling and large-scale production provide basis.

Description

Magnificent Paris polyphylla high efficiency seedling cultivating method
Technical field
The present invention relates to botanical seedling culturing technical field, and in particular to a kind of magnificent Paris polyphylla high efficiency seedling cultivating method.
Background technology
Magnificent Paris polyphylla (scientific name：Paris polyphylla), alias paris polyphylla, belong to Liliaceae (Liliaceae) Paris, is herbaceos perennial, and with clearing heat and detoxicating, swelling and pain relieving, effect of cool liver arresting convulsion is clinically usually used in malignant boil and swollen The treatment for diseases such as carbuncle swells, abscess of throat, venomous snake bite, the pain of injury caused by falling and tumbling, convulsion.
Paris plant medical value is high, and artificial excavation is serious, and natural resources faces exhaustion.Meanwhile, Paris polyphylla seed dormancy Time is long, need to could be sprouted through the summer in two winter one, and natural germination rate is low, seed needs 5~10 years or so from sprouting to being used as medicine, Resource regeneration is difficult.Therefore, the fast breeding for how realizing Paris polyphylla is a problem of current urgent need to resolve.
Current breeding mainly for Paris polyphylla has Propagation of Rhizomes, 3 kinds of modes of tissue cultures and seed growing.Tissue on Paris polyphylla Culture, researcher has done substantial amounts of work, but major part remains in the callus induction stage, is still used for big rule The nursery of mould.It is also successfully a kind of most widely used mode that Propagation of Rhizomes, which is presently the most, but still there is shortcoming and not Foot.First, rhizome is economic organ, can increase cost after cutting, and sowing quantity is big；Second, it need to largely reserve seed for planting, easily occur rotten It is rotten, and it is subject to plague of rats invasion and attack；3rd, though breeding coefficient is high, the cycle is long, it is impossible to meet production needs.
Seed growing is big due to breeding coefficient, and seed source is convenient, therefore as another in addition to Propagation of Rhizomes Important modes of reproduction, but Paris polyphylla seed is under field conditions (factors), need to can just be emerged by 2 winters, and natural germination rate is low, Therefore, break Paris polyphylla seed dormancy, improve seed germination rate, be that key in the urgent need to address during Paris polyphylla seed growing is asked Topic.
The content of the invention
To solve in the prior art, Paris polyphylla emergence rate is low, growing-seedling period length deficiency, and it is an object of the invention to provide one The method using magnificent Paris polyphylla seed high efficiency seedling cultivating is planted, basis is provided for the fast breeding and large-scale production of magnificent Paris polyphylla seedling.
The present invention is achieved through the following technical solutions, and is comprised the following steps：
（1）Seed selection, pretreatment：Selection is healthy and strong, the magnificent Paris polyphylla seed of no disease and pests harm, removes aril；
（2）First time deepfreeze：By the step（1）The middle Paris polyphylla seed for removing aril stores 30 under the conditions of 0 ~ 4 DEG C ~ 35 days；.
（3）First time ferment treatment：The step will be passed through（2）Paris polyphylla seed after processing under the conditions of 35 DEG C ~ 37 DEG C, in Handled 0.5 ~ 2.0 hour in mixed solution containing glusulase and D-sorbite；
（4）Second of deepfreeze：The step will be passed through（3）Paris polyphylla seed after processing stores 35 ~ 40 under the conditions of 0 ~ 4 DEG C My god；
（5）Second of ferment treatment：The step will be passed through（4）Paris polyphylla seed after processing is under the conditions of 35 DEG C ~ 37 DEG C, in 200 Handled 36 ~ 48 hours in the mg/L of mg/L ~ 350 Gibberellins solution；
（6）Sand is hidden：The step will be passed through（5）Paris polyphylla seed damp sand preservation under the conditions of 18 DEG C ~ 25 DEG C after processing, described in Paris polyphylla seed breach rate reaches 50% ~ 70%, waits to sow；
（7）Sowing：By the step（6）In Paris polyphylla seed to be sowed sow on seedbed, cover matrix, temperature control is 15 DEG C ~ 25 DEG C under the conditions of, insulation is passed the winter；
（8）Nursery is conserved：In humidity 75% ~ 85%, shade density under the conditions of 65% ~ 85%, treat that it is emerged；After emerging, timely weeding, Except pest and disease damage, plant growth regulator solution is sprayed.
In one embodiment, the step（3）In mixed solution in glusulase content be 60mg/L ~ 100mg/L, The content of D-sorbite is 10mg/L ~ 30mg/L.
In one embodiment, the step（3）It is the sorb that Paris polyphylla seed is placed in containing 10 mg/L to ~ 30 mg/L In the mixed solution of the mg/L glusulases of alcohol and 60 mg/L ~ 100, constant temperature bath 1 hour under the conditions of 37 DEG C is taken out and dries weight Building seed.
In one embodiment, the step（5）It is the gibberellin that Paris polyphylla seed is placed in the mg/L of 200 mg/L ~ 350 In solution, constant temperature is handled 36 ~ 48 hours under the conditions of 35 DEG C, then outwells Gibberellins solution, and the Paris polyphylla seed after processing is used Water is rinsed well.
In one embodiment, the step（7）In Paris polyphylla seed thickness of sowing be 700 ~ 1000/m2, and cover The cm of the cm of lid 1 ~ 3 or so matrix.
In one embodiment, the matrix includes fertile soil, the farm manure of fermentation maturity and thick river sand.
In one embodiment, percent by volume, fertile soil are pressed in the matrix：The farm manure of fermentation maturity：Thick river sand= 3~4:0.5~1:1 ~ 2, the matrix is handled by sterilization.
In one embodiment, the step（8）In plant growth regulator solution in include gibberellin, heteroauxin And brassin lactones.
The magnificent Paris polyphylla high efficiency seedling cultivating method of the present invention, imitates the process that Paris polyphylla seed is sprouted naturally, by pretreatment, twice Low-temperature treatment and twice ferment treatment, complete and are developed after magnificent Paris polyphylla seed, and effectively break the dormancy of Paris polyphylla seed, make Paris polyphylla Cycle time of emerging to 220 ~ 270 days, emergence rate reaches more than 85%, and seedling quality better, substantially reduces growing-seedling period, Improve emergence rate simultaneously.
Embodiment
Below in conjunction with the specific embodiment of the invention, clear, complete description is carried out to technical scheme.When So, described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based in the present invention Design and embodiment, the every other implementation that those of ordinary skill in the art are obtained under the premise of creative work is not made Example, belongs to protection scope of the present invention.
The magnificent Paris polyphylla high efficiency seedling cultivating method of the present embodiment, comprises the following steps：
（1）Seed selection, pretreatment：Selection is healthy and strong, the magnificent Paris polyphylla seed of no disease and pests harm, removes aril.
Specifically, taking the seed of the magnificent Paris polyphylla of growth more than 5 years, the red aril of Paris polyphylla seed is removed, breast is obtained The seed of white, and it is dried naturally.
（2）First time deepfreeze：By step（1）The middle Paris polyphylla seed for removing aril stores 30 under the conditions of 0 ~ 4 DEG C ~ 35 days.
（3）First time ferment treatment：Step will be passed through（2）Paris polyphylla seed after processing under the conditions of 35 DEG C ~ 37 DEG C, in containing Handled 0.5 ~ 2.0 hour in the mixed solution of glusulase and D-sorbite.Wherein, glusulase can be further by Paris polyphylla seed cell Wall it is broken, and sorbierite can provide active environment for glusulase, so as to be beneficial to the sprouting of Paris polyphylla seed.
Specifically, being by through step（2）The Paris polyphylla seed of processing be placed in containing 10 mg/L ~ 30 mg/L sorbierite and In the mixed solution of the mg/L glusulases of 60 mg/L ~ 100, constant temperature bath 1 hour under the conditions of 37 DEG C is taken out and dries Paris polyphylla kind Son.
Further, in mixed solution, the concentration of sorbierite is 20mg/L, and the content of glusulase is 80mg/L.
（4）Second of deepfreeze：Step will be passed through（3）Paris polyphylla seed after processing stores 35 ~ 40 under the conditions of 0 ~ 4 DEG C My god.
（5）Second of ferment treatment：Step will be passed through（4）Paris polyphylla seed after processing is under the conditions of 35 DEG C ~ 37 DEG C, in 200 Handled 36 ~ 48 hours in mg/L ~ 350mg/L Gibberellins solution.
Specifically, being the gibberellin that the Paris polyphylla seed by second of chilling treatment is placed in 200 mg/L ~ 350mg/L In solution, constant temperature is handled 36 ~ 48 hours under the conditions of 35 DEG C, then outwells Gibberellins solution, and the Paris polyphylla seed after processing is used Water is rinsed well.
（6）Sand is hidden：Step will be passed through（5）Paris polyphylla seed damp sand preservation under the conditions of 18 DEG C ~ 25 DEG C after processing, to Paris polyphylla Seed breach rate reaches 50% ~ 70%, waits to sow.
Further, the damp sand of sand Tibetan is by disinfecting, by the damp sand after disinfecting and Paris polyphylla kind Son by a certain percentage be well mixed after, the preservation under the conditions of 18 ~ 25 DEG C, to Paris polyphylla seed breach rate reach 60% and more than, treat Sowing is optimal.
（7）Sowing：By step（6）In Paris polyphylla seed to be sowed sow on seedbed, cover matrix, temperature control is 15 DEG C ~ 25 DEG C under the conditions of, insulation is passed the winter.
Further, the thickness of sowing of Paris polyphylla seed is 700 ~ 1000/m2, after planting cover cm of 1 cm ~ 3 or so Matrix.
Specifically, the matrix main component of covering Paris polyphylla seed is fertile soil, the farm manure of fermentation maturity and thick river sand.
Further, by volume percentage, fertile soil in matrix：The farm manure of fermentation maturity：Thick river sand=3 ~ 4:0.5~ 1:1 ~ 2, matrix need to be by sterilization processing.
（8）Nursery is conserved：In humidity 75% ~ 85%, shade density under the conditions of 65% ~ 85%, treat that it is emerged；After emerging, in time Weeding, except pest and disease damage, spray plant growth regulator solution.
Further, step（8）In except pest and disease damage method be sprinkling carbendazim solution 2 ~ 3 times, while use 1 ~ 2 time it is white stiff Bacterium is to reduce ground worm, and respectively at spraying before emerging and twice plant growth regulator solution after emerging.It is appreciated that for removing The medicine of pest and disease damage can also be that other can be used for the medicine of plant pest management, not limit herein.
Further, for the active ingredient in the plant growth regulator solution of nursery curing step comprising red mould Element, heteroauxin and brassin lactones etc..
Preferably, by mass percentage, gibberellin in plant growth regulator：Heteroauxin：Brassin lactones=100 ~ 300:1~2:1~1.5。
It is specific embodiment below
Embodiment 1
The present embodiment China Paris polyphylla seed seedling-raising method, including step in detailed below：
（1）Seed selection, pretreatment：More than 5 years wild magnificent Paris polyphylla imitating wild planting bases are selected in advance, in mid or late October then Picking fruit, is contained in closed container after removing shell, is placed in shady place storage.Treat that kind of skin occurs rotting taking out seed stranding Red aril, exposes milky seed, dries naturally.
（2）First time deepfreeze：With 0 ~ 4 DEG C of condition, the magnificent Paris polyphylla seed of storage 30 days.
（3）First time ferment treatment：The magnificent Paris polyphylla seed handled by first time deepfreeze is placed in containing 20 mg/L's 37 DEG C of constant temperature 1 hour in sorbitol solution and 80 mg/L snail enzyme solutions；Taking-up is dried.
（4）Second of deepfreeze：By step（3）Magnificent Paris polyphylla seed after drying is stored 35 days under the conditions of being placed in 0 ~ 4 DEG C.
（5）Second of ferment treatment：The seed after second of refrigeration is taken out, in 200 mg/L Gibberellins solution, 35 Constant temperature is handled 36 hours under the conditions of DEG C, outwells Gibberellins solution, and divide 3 times by magnificent Paris polyphylla with the clear water of 10 times of soak volumes Seed is rinsed well.
（6）Sand is hidden：With the thick river sand and Paris polyphylla seed that are exposed to the sun and sterilize according to 2:1 ratio uniform mixing, at 18 ~ 25 DEG C Damp sand under the conditions of reach 60% to seed breach rate, wait to sow.
（7）Sowing：There to be breach seed to be sowed according to 700 ~ 1000/m2Density, uniform broadcasting on seedbed, and Cover 2cm or so matrix.Ground canopy insulation is done with black mocromembrane to pass the winter.
（8）Nursery is conserved：
Late Febuary in next year, spray concentration is the green shield solution of 50ppm；Roof insulating is removed at the beginning of 3 months, its reform of nature condition is allowed, holding Humidity 75% ~ 85%, shade density treats that it is emerged more than 65%.
Green shield solution is that gained, wherein active ingredient gibberellin are bought in market：Heteroauxin：Brassin lactones=1350：5： 3, it is a kind of plant growth regulator of synthesis, early stage is conducive to induction to sprout, and the later stage can reach the effect of shield bud strengthening root.
April will emerge successively, and now spray concentration is 30ppm green shield solution；Late May, which emerges, to be basically completed；Go out Accomplish to see that grass is just removed between seedling stage, and sprayed 2 times using carbendazim solution, while to reduce the worm using 1 muscardine.
In this example, the magnificent Paris polyphylla seed sprouting cycle, emergence rate was up to 90% at 220 days or so.
Embodiment 2
The present embodiment China Paris polyphylla seed seedling-raising method, including step in detailed below：
（1）Seed selection, pretreatment：More than 5 years wild magnificent Paris polyphylla imitating wild planting bases are selected in advance, are adopted in early November then Fruit is received, is contained in after removing shell in closed container, shady place storage is placed in.Plant skin and occur rotting being to take out seed stranding to go red Aril, exposes milky seed, dries naturally.
（2）First time deepfreeze：With 0 ~ 4 DEG C of condition, the magnificent Paris polyphylla seed of storage 35 days.
（3）First time ferment treatment：Seed is placed in 37 DEG C of constant temperature 1 in 10mg/L sorbitol solution, 60ppm snail enzyme solutions small When；Taking-up is dried.
（4）Second of deepfreeze：By step（3）Magnificent Paris polyphylla seed after drying is stored 40 days under the conditions of being placed in 0 ~ 4 DEG C.
（5）Second of ferment treatment：The seed after second of refrigeration is taken out, in 350mg/L Gibberellins solution, at 35 DEG C Under the conditions of, constant temperature is handled 48 hours, outwells Gibberellins solution, and clear water rinses Paris polyphylla seed well.
（6）Sand is hidden：With the thick river sand and Paris polyphylla seed that are exposed to the sun and sterilize according to 2:1 ratio uniform mixing, at 18 ~ 25 DEG C Damp sand under the conditions of reach more than 50% to seed breach rate, wait to sow.
（7）Sowing：There to be breach and the seed of white silk occur according to 700 ~ 1000/m2Density, uniform broadcasting is in seedbed On, and cover 2cm or so matrix.Ground canopy insulation is done with black mocromembrane to pass the winter.
（8）Nursery is conserved：
Late Febuary in next year, spray concentration is gibberellin in 50ppm plant growth regulator solution, plant growth regulator solution： Heteroauxin：Brassin lactones=100 ~ 300: 1~2 : 1~1.5；Roof insulating is removed at the beginning of 3 months, its reform of nature condition is allowed, Humidity 75% ~ 85% is kept, shade density treats that it is emerged more than 65%.
April emerges successively, and now spray concentration is 30 ~ 50ppm plant growth regulator solution, plant growth regulating Gibberellin in agent solution：Heteroauxin：Brassin lactones=100 ~ 300: 1~2 : 1~1.5；Late May emerges substantially complete Into；Accomplish during emerging to see that grass is just removed, and sprayed 2 ~ 3 times using carbendazim solution, while using 1 ~ 2 muscardine to reduce ground Worm.
The present embodiment, the magnificent Paris polyphylla seed sprouting cycle, emergence rate reached more than 85% at 270 days or so.
Embodiment 3
The seed high efficiency seedling cultivating method of this implementation China Paris polyphylla, is comprised the following steps that：
（1）Seed selection and pretreatment：More than 5 years wild magnificent Paris polyphylla imitating wild planting bases are selected in advance, in mid or late October then Picking fruit, is contained in closed container after removing shell, is placed in shady place storage.Kind of skin occur rotting taking out seed rub with the hands go it is red Color aril, exposes milky seed, dries naturally.
（2）First time deepfreeze：With 0 ~ 4 DEG C of condition, the magnificent Paris polyphylla seed of storage 32 days.
（3）First time ferment treatment：Seed is placed in 30 mg/L sorbitol solution, 100 ppm snail enzyme solutions 37 DEG C After constant temperature 1 hour, taking-up is dried.
（4）Second of deepfreeze：By step（3）Magnificent Paris polyphylla seed after drying stores day under the conditions of being placed in 0 ~ 4 DEG C.
（5）Second of ferment treatment：The seed after second of refrigeration is taken out, in 285 mg/L Gibberellins solution, 35 Under the conditions of DEG C, constant temperature is handled 42 hours, outwells Gibberellins solution, and planted with 4 cleanings of clear water point of 10 times of soak volumes Son.
（6）Sand is hidden：With the thick river sand and Paris polyphylla seed that are exposed to the sun and sterilize according to 2:1 ratio uniform mixing, at 18 ~ 25 DEG C Damp sand under the conditions of reach more than 60% to seed breach rate, wait to sow.
（7）Sowing：By fatiscent seed according to 700 ~ 1000/m2Density, uniform broadcasting is covered on seedbed 2cm or so matrix.Ground canopy insulation is done with black mocromembrane to pass the winter.
（8）Nursery is conserved：
Late Febuary in next year, spray concentration is the green shield solution of 50ppm；Roof insulating is removed at the beginning of 3 months, its reform of nature condition is allowed, holding Humidity 75% ~ 85%, shade density treats that it is emerged more than 65%.
April emerges successively, and now spray concentration is 40ppm green shield solution；Late May, which emerges, to be basically completed；Emerge Period accomplishes to see that grass is just removed, and is sprayed 2 ~ 3 times using carbendazim solution, while to reduce the worm using 1 ~ 2 muscardine.
The present embodiment, the magnificent Paris polyphylla seed sprouting cycle, emergence rate was up to more than 87% at 240 days or so.
All of above embodiment, prepares seedling medium, matrix formulations are as follows in harvesting seed simultaneously：More than 700 meters of height above sea level Mountain forest fertile soil（15cm endosexines soil, is sterilized with 800 times of 45% carbendazim）, fully ferment ripe farm manure, sunlight exposure cross Thick river sand, by volume 4:1:2, while adding muscardine powder（It is 20g/m with reference to ratio3）Sterilization, after being well mixed It is stand-by.
Embodiment described above is only presently preferred embodiments of the present invention, is not intended to limit the invention, all in this hair Any modification, equivalent substitution and improvements made within bright spirit and principle etc., should be included in protection scope of the present invention it It is interior.

Claims (8)

1. a kind of method of magnificent Paris polyphylla high efficiency seedling cultivating, it is characterised in that comprise the following steps：
（1）Seed selection, pretreatment：The Paris polyphylla seed of maturation is taken, aril is removed；
（2）First time low-temperature preservation：By the step（1）The middle Paris polyphylla seed for removing aril stores 30 under the conditions of 0 ~ 4 DEG C ~ 35 days；
（3）First time ferment treatment：The step will be passed through（2）Paris polyphylla seed after processing under the conditions of 35 DEG C ~ 37 DEG C, in containing Handled 0.5 ~ 2.0 hour in the mixed solution of glusulase and D-sorbite；
（4）Second of low-temperature preservation：The step will be passed through（3）Paris polyphylla seed after processing stores 35 ~ 40 under the conditions of 0 ~ 4 DEG C My god；
（5）Second of ferment treatment：The step will be passed through（4）Paris polyphylla seed after processing is under the conditions of 35 DEG C ~ 37 DEG C, in 200 Handled 36 ~ 48 hours in mg/L ~ 350mg/L Gibberellins solution；
（6）Sand is hidden：The step will be passed through（5）Paris polyphylla seed damp sand preservation under the conditions of 18 ~ 25 DEG C after processing, it is extremely described heavy Building seed breach rate reaches 50% ~ 70%, waits to sow；
（7）Sowing：By the step（6）In Paris polyphylla seed to be sowed sow on seedbed, cover matrix, temperature control is 15 DEG C ~ 25 DEG C under the conditions of, insulation is passed the winter；
（8）Nursery is conserved：In humidity 75% ~ 85%, shade density under the conditions of 65% ~ 85%, treat that it is emerged；After emerging, timely weeding, Except pest and disease damage, plant growth regulator solution is sprayed.
2. the method for magnificent Paris polyphylla high efficiency seedling cultivating according to claim 1, it is characterised in that the step（3）In mixing The content of glusulase is the mg/L of 60 mg/L ~ 100 in solution, and the content of D-sorbite is the mg/L of 10 mg/L ~ 30.
3. the method for magnificent Paris polyphylla high efficiency seedling cultivating according to claim 2, it is characterised in that the step（3）It is by Paris polyphylla Seed is placed in containing 10 mg/L in the mixed solution of the mg/L glusulases of ~ 30 mg/L sorbierite and 60 mg/L ~ 100, Constant temperature bath 1 hour under the conditions of 37 DEG C, takes out and dries Paris polyphylla seed.
4. the method for magnificent Paris polyphylla high efficiency seedling cultivating according to claim 1, it is characterised in that the step（5）It is by Paris polyphylla Seed is placed in the mg/L of 200 mg/L ~ 350 Gibberellins solution, and constant temperature is handled 36 ~ 48 hours under the conditions of 35 DEG C, then is fallen Fall Gibberellins solution, and the Paris polyphylla seed after processing is rinsed well with water.
5. the method for magnificent Paris polyphylla high efficiency seedling cultivating according to claim 1, it is characterised in that the step（7）In Paris polyphylla The thickness of sowing of seed is 700 ~ 1000/m2, and cover the thick matrix of the cm of 1 cm ~ 3.
6. the method for magnificent Paris polyphylla high efficiency seedling cultivating according to claim 5, it is characterised in that the matrix include fertile soil, The farm manure of fermentation maturity and thick river sand.
7. the method for magnificent Paris polyphylla high efficiency seedling cultivating according to claim 6, it is characterised in that volume basis is pressed in the matrix Than fertile soil：The farm manure of fermentation maturity：Thick river sand=3 ~ 4:0.5~1:1 ~ 2, the matrix is handled by sterilization.
8. the method for magnificent Paris polyphylla high efficiency seedling cultivating according to claim 1, it is characterised in that the step（8）In plant Gibberellin, heteroauxin and brassin lactones are included in growth regulating agent solution.
CN201710496075.7A 2017-06-26 2017-06-26 Magnificent Paris polyphylla high efficiency seedling cultivating method Pending CN107182521A (en)

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CN113994790A (en) * 2021-11-09 2022-02-01 广西壮族自治区中国科学院广西植物研究所 Method for promoting rapid germination and seedling emergence of paris polyphylla rhizome buds

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Application publication date: 20170922