CN109601061A - A kind of oil tree peony scale breeding seed accelerating germination method - Google Patents

A kind of oil tree peony scale breeding seed accelerating germination method Download PDF

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Publication number
CN109601061A
CN109601061A CN201910081890.6A CN201910081890A CN109601061A CN 109601061 A CN109601061 A CN 109601061A CN 201910081890 A CN201910081890 A CN 201910081890A CN 109601061 A CN109601061 A CN 109601061A
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seed
tree peony
fruit
oil tree
scale breeding
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Chinese (zh)
Inventor
黄治民
张娜
刘冰
邵清良
付晓
袁新征
崔丽雅
李雯
赵璐
韩宁静
韩志强
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Luoyang Peony Development Center
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Luoyang Peony Development Center
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Priority to CN201910081890.6A priority Critical patent/CN109601061A/en
Publication of CN109601061A publication Critical patent/CN109601061A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/02Germinating apparatus; Determining germination capacity of seeds or the like
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08Immunising seed

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  • Life Sciences & Earth Sciences (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The present invention relates to industry of planting forest or fruit tress technical field, specially a kind of oil tree peony scale breeding seed accelerating germination method, steps are as follows: seed collection: Cai Shou Follicle radish shell is in isabelline or brown, seed milk yellow or rufous Follicle radish fruit;After the harvesting of seed treatment: Follicle radish fruit, it spreads in the interior of shady and cool ventilation, allows its after-ripening, be sure not exposure, it was stirred every 1-2 days primary, by 10-15 days, fruit pod gradually became brown or black, and most of fruit pods voluntarily crack, produce seed, minority can be stripped manually, then remove fruit pod, sort out seed;It broadcasts preceding vernalization: presoaking and germinating processing being carried out to seed with clear water prior to seeding, make kind of soft and soggyization, water swelling is easy to rudiment;Seed: being transported to the nursery put in order by sowing, with dedicated tree peony planting with sowing machine, 80-double centner of application rate per acre.Breeding coefficient can be effectively improved using method of the invention, increase nursery amount, provide high quality seedling for oil tree peony industry great development.

Description

A kind of oil tree peony scale breeding seed accelerating germination method
Technical field
The present invention relates to industry of planting forest or fruit tress technical field, specifically a kind of oil tree peony scale breeding seed accelerating germination method.
Background technique
Industry of planting forest or fruit tress (including the woods, gardens, fruit tree, flowers) is the important component of agricultural structure, and is realized The industry that ecological benefits and economic benefit are laid equal stress on.In modern society, it is plentiful, national that flourishing industry of planting forest or fruit tress is often considered as country by people Flourishing and social civilization one of mark, oil uses tree peony frequently with seeding and seedling raising method, currently, oil enters extensive development with tree peony Phase, cultivated area quickly increase, and high-quality strong sprout demand is big.In order to meet the market demand, scale breeding, which becomes, solves seedling One effective way of problem.Scale breeding needs seed amount big, and the quality height that Accelerating germination before sowing link carries out determines seedling Output of seedling and later period growth and development situation.
Being badly in need of one kind now can be improved breeding coefficient, increase nursery amount, provide for oil tree peony industry great development high-quality The oil of seedling tree peony scale breeding seed accelerating germination method.
Summary of the invention
The purpose of the present invention is to provide a kind of oil tree peony scale breeding seed accelerating germination methods, to solve above-mentioned background The problem of being proposed in technology.
To achieve the above object, the invention provides the following technical scheme:
A kind of oil tree peony scale breeding seed accelerating germination method, steps are as follows: (1), seed collection: Cai Shou Follicle radish shell is in brown Yellow or brown, seed milk yellow or rufous Follicle radish fruit;(2), it after the harvesting of seed treatment: Follicle radish fruit, spreads logical in cool place The interior of wind, allows its after-ripening, is sure not exposure, stirred every 1-2 days it is primary, by 10-15 days, fruit pod gradually become brown or Black, most of fruit pods voluntarily crack, and produce seed, and minority can be stripped manually, then remove fruit pod, sort out seed;(3), It broadcasts preceding vernalization: presoaking and germinating processing being carried out to seed with clear water prior to seeding, make kind of soft and soggyization, water swelling is easy to rudiment; (4), it sows: seed is transported to the nursery put in order, with dedicated tree peony planting with sowing machine, 80-double centner of application rate per acre.
As a further solution of the present invention: to eliminate influence of the shrivelled seed to germination percentage in step (2), can will sort out Seed be put into clear water, impregnate 1-2 hour after, remove the sundries to float on the surface and shrivelled seed, sink in water intaking Full seed is to sow.
As a further solution of the present invention: the seed sorted out in step (2) is placed in the sun in the time prior to seeding Light is unable to the place of direct projection, spreads preservation out in the cool, and seed spreads thickness out lower than 10cm.
As a further solution of the present invention: specific germination accelerating method is as follows in the step (3): 1. building reservoir: using A brick high 80cm out, each 5 meters of length and width of the interim pond of square, is laid on bottom of pond portion with colour bar cloth, while to surround four The brick wall in week after completing, puts clear water depth 50cm to prevent pond leak;2. seed impurity removing: will be in seed with small-sized winnowing machine Impurity further removes, and makes seed without pericarp and dust as far as possible;3. Steeping and budding: the seed after removal of impurities being poured into pond, is used Dedicated plastics, which lift, shakeouts seed, primary every stirring in 2 hours, after 24 hours, by the impurity floated on above and is done with strainer Flat seed is pulled out, and clear water is then changed, and repetition in the 2nd and the 3rd day operates on the 1st day.
As a further solution of the present invention: after Steeping and budding the 3rd day is put into clear water, admixing 1000 grams of carbendazim 50% Wettable powder carries out sterilizing to seed.
As a further solution of the present invention: the 4th clear water is changed within Steeping and budding the 5th day, while carbendazim is added again The gibberellin GA of 200 grams of 200PM is added.
As a further solution of the present invention: taking out several seeds at random within Steeping and budding the 7th day, kernel is snow-white crisp after incision Tender, close with kind of bark graft touching, plumule expands, and vernalization is completed, and then pulls soaked seed out, In Shade to dry table Wait sow after the severe edema due to hypofunction of the spleen point.
Compared with prior art, the beneficial effects of the present invention are: breeding coefficient can be effectively improved using method of the invention, Increase nursery amount, provides high quality seedling for oil tree peony industry great development.
Specific embodiment
It should be noted that in the absence of conflict, the feature in embodiment and embodiment in the present invention can phase Mutually combination.
Below in conjunction with embodiment, the present invention will be described in detail.
Embodiment one
A kind of oil tree peony scale breeding seed accelerating germination method, steps are as follows:
1, seed collection
The fruit Wei Follicle radish fruit of tree peony, a fruit generally have 4-5 fruit pods, and 6-10 seeds are contained in each fruit pod. The harvesting of Paeonia suffruticosa seed will harvest in batches in due course, can not too early or too late.Too early seed is immature, and quality is tender and aqueous more, holds It easily goes rotten or shrivelled;Seed blackening too late, matter is hard, plants skin depth, and the maturity of seed influences very big, general Follicle of Danging to germination percentage Radish shell is in isabelline or brown, and when seed milk yellow or rufous will harvest.The mass of 1000 kernel of Paeonia suffruticosa seed is about 150g, institute With the breeding coefficient of seed propagation is larger, can obtain a large amount of high-quality tree peony seedlings in a short time.
2, seed treatment
After the harvesting of Follicle radish fruit, it should spread in the interior of shady and cool ventilation, allow its after-ripening.It is sure not exposure, otherwise seed is once drying, just Germinating capacity, the unsuitable overdrying in the place of Tan Fang Follicle radish fruit or humidity can be lost.Too dry, one layer of juice in shell can be very It is fast to dry out, seed cannot abundant after-ripening, or even cause to be dehydrated, seed is shrivelled;It is excessively moist, it will cause fever or go rotten.It should be every It stirred every 1-2 days once, goes rotten to prevent inner heat.By 10 days, fruit pod gradually became brown or black, most of fruit pods It voluntarily cracks, produces seed, minority can be stripped manually, then remove fruit pod, sort out seed.To eliminate shrivelled seed to germination The seed sorted out can be put into clear water by the influence of rate, after impregnating 1 hour, remove the sundries to float on the surface and shrivelled kind Son, the full seed to sink in water intaking is to sow.Oil is sowed with tree peony and is generally carried out in mid-September (White Dew solar term), is passed through The full maturated oil Paeonia suffruticosa seed screened in time prior to seeding, should be placed in the place that sunlight is unable to direct projection, Shady place spreads preservation out, and seed spreads thickness out no more than 10cm.
3, preceding vernalization is broadcast
Oil screens fuller mature seed per kilogram up to 2600~3000 with tree peony, and 1 kilogram of dry kind of leaching 24 is small When can absorb water 1.2 kilograms.The permeable and gas permeability of tree peony kind skin is to influence an important factor for seed is sprouted, oily Paeonia suffruticosa seed kind Skin is not only black but also hard, it is difficult to which permeable, scale breeding carries out presoaking and germinating processing to seed with clear water prior to seeding, keeps kind soft and soggy Change, water swelling is easy to rudiment.Specific germination accelerating method is as follows:
1. building reservoir.With a brick high 80cm out, the interim pond of each 5 meters of length and width of square (can handle 3000 kilograms How much seed increases length and width according to seed), it is laid on bottom of pond portion with colour bar cloth, while to surround the brick wall of surrounding, to prevent pond Leak.After completing, clear water depth 50cm is put.
2. seed impurity removing.The impurity in seed is further removed with small-sized winnowing machine, makes seed without pericarp and dirt as far as possible Soil.
3. Steeping and budding.Seed after removal of impurities is poured into pond, is lifted with dedicated plastics and shakeouts seed.It is small every 2 When stirring it is primary.After 24 hours, impurity above will be floated on strainer and shrivelled seed is pulled out, then changes clear water.2nd and Repetition in 3 days operates on the 1st day, after the 3rd day is put into clear water, admixes 1000 grams of 50% wettable powder of carbendazim, sterilizes to seed Disinfection.The 4th clear water is changed within 5th day, GA200 grams of gibberellin of 200PM is added while carbendazim is added again, the 7th day random Several seeds are taken out, kernel is snow-white tender and crisp after incision, and close with kind of bark graft touching, plumule expands, and vernalization is completed.It will then impregnate Good seed is pulled out and (should not first discharge water fish out seed afterwards at this time), In Shade to dry after transepidermal water wait sow.
4, it sows
Seed is transported to the nursery put in order, with dedicated tree peony planting with sowing machine, 80 kilograms of application rate per acre, output of seedling per acre Up to 200,000 plants.
Embodiment two
A kind of oil tree peony scale breeding seed accelerating germination method, steps are as follows:
1, seed collection
The fruit Wei Follicle radish fruit of tree peony, a fruit generally have 4-5 fruit pods, and 6-10 seeds are contained in each fruit pod. The harvesting of Paeonia suffruticosa seed will harvest in batches in due course, can not too early or too late.Too early seed is immature, and quality is tender and aqueous more, holds It easily goes rotten or shrivelled;Seed blackening too late, matter is hard, plants skin depth, and the maturity of seed influences very big, general Follicle of Danging to germination percentage Radish shell is in isabelline or brown, and when seed milk yellow or rufous will harvest.The mass of 1000 kernel of Paeonia suffruticosa seed is about 180g, institute With the breeding coefficient of seed propagation is larger, can obtain a large amount of high-quality tree peony seedlings in a short time.
2, seed treatment
After the harvesting of Follicle radish fruit, it should spread in the interior of shady and cool ventilation, allow its after-ripening.It is sure not exposure, otherwise seed is once drying, just Germinating capacity, the unsuitable overdrying in the place of Tan Fang Follicle radish fruit or humidity can be lost.Too dry, one layer of juice in shell can be very It is fast to dry out, seed cannot abundant after-ripening, or even cause to be dehydrated, seed is shrivelled;It is excessively moist, it will cause fever or go rotten.It should be every It stirred every 1-2 days once, goes rotten to prevent inner heat.By 15 days, fruit pod gradually became brown or black, most of fruit pods It voluntarily cracks, produces seed, minority can be stripped manually, then remove fruit pod, sort out seed.To eliminate shrivelled seed to germination The seed sorted out can be put into clear water by the influence of rate, after impregnating 2 hours, remove the sundries to float on the surface and shrivelled kind Son, the full seed to sink in water intaking is to sow.Oil is sowed with tree peony and is generally carried out in mid-September (White Dew solar term), is passed through The full maturated oil Paeonia suffruticosa seed screened in time prior to seeding, should be placed in the place that sunlight is unable to direct projection, Shady place spreads preservation out, and seed spreads thickness out no more than 10cm.
3, preceding vernalization is broadcast
Oil screens fuller mature seed per kilogram up to 2600~3000 with tree peony, and 1 kilogram of dry kind of leaching 24 is small When can absorb water 1.2 kilograms.The permeable and gas permeability of tree peony kind skin is to influence an important factor for seed is sprouted, oily Paeonia suffruticosa seed kind Skin is not only black but also hard, it is difficult to which permeable, scale breeding carries out presoaking and germinating processing to seed with clear water prior to seeding, keeps kind soft and soggy Change, water swelling is easy to rudiment.Specific germination accelerating method is as follows:
1. building reservoir.With a brick high 80cm out, the interim pond of each 5 meters of length and width of square (can handle 3000 kilograms How much seed increases length and width according to seed), it is laid on bottom of pond portion with colour bar cloth, while to surround the brick wall of surrounding, to prevent pond Leak.After completing, clear water depth 50cm is put.
2. seed impurity removing.The impurity in seed is further removed with small-sized winnowing machine, makes seed without pericarp and dirt as far as possible Soil.
3. Steeping and budding.Seed after removal of impurities is poured into pond, is lifted with dedicated plastics and shakeouts seed.It is small every 2 When stirring it is primary.After 24 hours, impurity above will be floated on strainer and shrivelled seed is pulled out, then changes clear water.2nd and Repetition in 3 days operates on the 1st day, after the 3rd day is put into clear water, admixes 1000 grams of 50% wettable powder of carbendazim, sterilizes to seed Disinfection.The 4th clear water is changed within 5th day, GA200 grams of gibberellin of 200PM is added while carbendazim is added again, the 7th day random Several seeds are taken out, kernel is snow-white tender and crisp after incision, and close with kind of bark graft touching, plumule expands, and vernalization is completed.It will then impregnate Good seed is pulled out and (should not first discharge water fish out seed afterwards at this time), In Shade to dry after transepidermal water wait sow.
4, it sows
Seed is transported to the nursery put in order, with dedicated tree peony planting with sowing machine, 90 kilograms of application rate per acre, output of seedling per acre Up to 200,000 plants.
Embodiment three
A kind of oil tree peony scale breeding seed accelerating germination method, steps are as follows:
1, seed collection
The fruit Wei Follicle radish fruit of tree peony, a fruit generally have 4-5 fruit pods, and 6-10 seeds are contained in each fruit pod. The harvesting of Paeonia suffruticosa seed will harvest in batches in due course, can not too early or too late.Too early seed is immature, and quality is tender and aqueous more, holds It easily goes rotten or shrivelled;Seed blackening too late, matter is hard, plants skin depth, and the maturity of seed influences very big, general Follicle of Danging to germination percentage Radish shell is in isabelline or brown, and when seed milk yellow or rufous will harvest.The mass of 1000 kernel of Paeonia suffruticosa seed is about 165g, institute With the breeding coefficient of seed propagation is larger, can obtain a large amount of high-quality tree peony seedlings in a short time.
2, seed treatment
After the harvesting of Follicle radish fruit, it should spread in the interior of shady and cool ventilation, allow its after-ripening.It is sure not exposure, otherwise seed is once drying, just Germinating capacity, the unsuitable overdrying in the place of Tan Fang Follicle radish fruit or humidity can be lost.Too dry, one layer of juice in shell can be very It is fast to dry out, seed cannot abundant after-ripening, or even cause to be dehydrated, seed is shrivelled;It is excessively moist, it will cause fever or go rotten.It should be every It stirred every 1-2 days once, goes rotten to prevent inner heat.By 12 days, fruit pod gradually became brown or black, most of fruit pods It voluntarily cracks, produces seed, minority can be stripped manually, then remove fruit pod, sort out seed.To eliminate shrivelled seed to germination The seed sorted out can be put into clear water by the influence of rate, after impregnating 1.5 hours, remove the sundries to float on the surface and shrivelled Seed, the full seed to sink in water intaking is to sow.Oil is sowed with tree peony and is generally carried out in mid-September (White Dew solar term), is passed through The full maturated oil Paeonia suffruticosa seed screened is crossed, in the time prior to seeding, the place that sunlight is unable to direct projection should be placed in, Spread preservation out in the cool, seed spreads thickness out no more than 10cm.
3, preceding vernalization is broadcast
Oil screens fuller mature seed per kilogram up to 2600~3000 with tree peony, and 1 kilogram of dry kind of leaching 24 is small When can absorb water 1.2 kilograms.The permeable and gas permeability of tree peony kind skin is to influence an important factor for seed is sprouted, oily Paeonia suffruticosa seed kind Skin is not only black but also hard, it is difficult to which permeable, scale breeding carries out presoaking and germinating processing to seed with clear water prior to seeding, keeps kind soft and soggy Change, water swelling is easy to rudiment.Specific germination accelerating method is as follows:
1. building reservoir.With a brick high 80cm out, the interim pond of each 5 meters of length and width of square (can handle 3000 kilograms How much seed increases length and width according to seed), it is laid on bottom of pond portion with colour bar cloth, while to surround the brick wall of surrounding, to prevent pond Leak.After completing, clear water depth 50cm is put.
2. seed impurity removing.The impurity in seed is further removed with small-sized winnowing machine, makes seed without pericarp and dirt as far as possible Soil.
3. Steeping and budding.Seed after removal of impurities is poured into pond, is lifted with dedicated plastics and shakeouts seed.It is small every 2 When stirring it is primary.After 24 hours, impurity above will be floated on strainer and shrivelled seed is pulled out, then changes clear water.2nd and Repetition in 3 days operates on the 1st day, after the 3rd day is put into clear water, admixes 1000 grams of 50% wettable powder of carbendazim, sterilizes to seed Disinfection.The 4th clear water is changed within 5th day, GA200 grams of gibberellin of 200PM is added while carbendazim is added again, the 7th day random Several seeds are taken out, kernel is snow-white tender and crisp after incision, and close with kind of bark graft touching, plumule expands, and vernalization is completed.It will then impregnate Good seed is pulled out and (should not first discharge water fish out seed afterwards at this time), In Shade to dry after transepidermal water wait sow.
4, it sows
Seed is transported to the nursery put in order, with dedicated tree peony planting with sowing machine, 80-double centner of application rate per acre, per acre Output of seedling is up to 200,000 plants.
Breeding coefficient can be effectively improved using method of the invention, increase nursery amount, mentioned for oil tree peony industry great development For high quality seedling.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.

Claims (7)

1. a kind of oil tree peony scale breeding seed accelerating germination method, which is characterized in that steps are as follows:
(1), seed collection: Cai Shou Follicle radish shell is in isabelline or brown, seed milk yellow or rufous Follicle radish fruit;
(2), it seed treatment: after the harvesting of Follicle radish fruit, spreads in the interior of shady and cool ventilation, allows its after-ripening, be sure not exposure, every 1-2 It is stirred once, and by 10-15 days, fruit pod gradually became brown or black, and most of fruit pods voluntarily crack, and produces seed, few Number can be stripped manually, then remove fruit pod, sort out seed;
(3), it broadcasts preceding vernalization: presoaking and germinating processing being carried out to seed with clear water prior to seeding, make kind of soft and soggyization, water swelling is easy In rudiment;
(4), it sows: seed is transported to the nursery put in order, with dedicated tree peony planting with sowing machine, application rate 80-100 per acre Kilogram.
2. a kind of oil tree peony scale breeding presprouting of seeds side according to claim 1, which is characterized in that step (2) In to eliminate the influence of shrivelled seed to germination percentage, the seed sorted out can be put into clear water, after impregnating 1-2 hour, be removed The sundries and shrivelled seed to float on the surface, the full seed to sink in water intaking is to sow.
3. a kind of oil tree peony scale breeding presprouting of seeds side according to claim 1 or 2, which is characterized in that step (2) seed sorted out in the time prior to seeding, is placed in the place that sunlight is unable to direct projection, spreads preservation out in the cool, kind Son spreads thickness out lower than 10cm.
4. a kind of oil tree peony scale breeding presprouting of seeds side according to claim 3, which is characterized in that the step (3) specific germination accelerating method is as follows in: 1. building reservoir: with a brick high 80cm out, each 5 meters of length and width of the interim water of square Pond is laid on bottom of pond portion with colour bar cloth, while to surround the brick wall of surrounding, to prevent pond leak, after completing, puts clear water depth 50cm;2. seed impurity removing: further removing the impurity in seed with small-sized winnowing machine, make seed without pericarp and dust as far as possible; 3. Steeping and budding: the seed after removal of impurities being poured into pond, is lifted with dedicated plastics and shakeouts seed, stirred one every 2 hours It is secondary, after 24 hours, impurity above will be floated on strainer and shrivelled seed is pulled out, then change clear water, repetition in the 2nd and the 3rd day It operates within 1st day.
5. a kind of oil tree peony scale breeding presprouting of seeds side according to claim 4, which is characterized in that Steeping and budding After 3rd day is put into clear water, 1000 grams of 50% wettable powder of carbendazim is admixed, sterilizing is carried out to seed.
6. a kind of oil tree peony scale breeding presprouting of seeds side according to claim 5, which is characterized in that Steeping and budding The 4th clear water is changed within 5th day, the gibberellin GA of 200 grams of 200PM is added while carbendazim is added again.
7. a kind of oil tree peony scale breeding presprouting of seeds side according to claim 6, which is characterized in that Steeping and budding Several seeds are taken out at random within 7th day, kernel is snow-white tender and crisp after incision, and close with kind of bark graft touching, plumule expands, and vernalization is completed, Then soaked seed is pulled out, it is In Shade to dry after transepidermal water wait sow.
CN201910081890.6A 2019-01-28 2019-01-28 A kind of oil tree peony scale breeding seed accelerating germination method Pending CN109601061A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110214622A (en) * 2019-07-05 2019-09-10 武威市嘉农科技有限责任公司 A kind of method for culturing seedlings improving diversiform-leaved poplar survival rate
CN112136412A (en) * 2020-10-08 2020-12-29 菏泽市国喜牡丹苗木有限公司 Breeding method of peony seeds

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104920049A (en) * 2015-06-30 2015-09-23 铜陵凤威农业科技有限公司 Method for cultivating seedlings of oil peonies
CN105309263A (en) * 2014-07-31 2016-02-10 江苏省农业科学院 Carya illinoensis container seedling water content management method
WO2019006466A1 (en) * 2017-06-30 2019-01-03 Booshoot Llc Compositions and methods for large-scale in vitro plant bioculture

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105309263A (en) * 2014-07-31 2016-02-10 江苏省农业科学院 Carya illinoensis container seedling water content management method
CN104920049A (en) * 2015-06-30 2015-09-23 铜陵凤威农业科技有限公司 Method for cultivating seedlings of oil peonies
WO2019006466A1 (en) * 2017-06-30 2019-01-03 Booshoot Llc Compositions and methods for large-scale in vitro plant bioculture

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110214622A (en) * 2019-07-05 2019-09-10 武威市嘉农科技有限责任公司 A kind of method for culturing seedlings improving diversiform-leaved poplar survival rate
CN112136412A (en) * 2020-10-08 2020-12-29 菏泽市国喜牡丹苗木有限公司 Breeding method of peony seeds

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Application publication date: 20190412