CN108718594A - A kind of hormone treatment method for improving Panax notoginseng seeds germination rate and shortening latter stage of ripening - Google Patents
A kind of hormone treatment method for improving Panax notoginseng seeds germination rate and shortening latter stage of ripening Download PDFInfo
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- CN108718594A CN108718594A CN201810417580.2A CN201810417580A CN108718594A CN 108718594 A CN108718594 A CN 108718594A CN 201810417580 A CN201810417580 A CN 201810417580A CN 108718594 A CN108718594 A CN 108718594A
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- seed
- panax notoginseng
- latter stage
- germination rate
- shortening
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Abstract
A kind of hormone treatment method for improving Panax notoginseng seeds germination rate and shortening latter stage of ripening provided by the invention, includes the following steps：Clear water is cleaned after choosing triennial plant maturation, the artificial peeling of full Panax notoginseng seeds, uses 5%CuSO4Germicidal solution soaking disinfection, then wash twice, interior dries in the shade to obtain clean seed, the gibberellin (GA of a concentration of 500mg/L of use quality3) the clean seed of solution immersion, the seed and seed-soaking liquid press 1:For 24 hours, clear water rinses two and dries to obtain clean seed inferior to interior 3~4 ratio seed soakings；The clean seed and moistening river sand are mixed thoroughly, the sand bed product interior at 4~15 DEG C is placed in ventilative good full mesh basket and is protected from light preservation 50~60 days.Through 500mg/L GA3Have there is kind of a skin cracking in 40 days Some seeds of seed of seed soaking, start to sprout；60 days embryo rates reach 80.7 ± 7.6%, and embryo rate is apparently higher than the 63.6 ± 7.5% of the control group of clear water immersion treatment.The present invention is remarkably improved Panax notoginseng seeds germination rate, and breaking dormancy in advance, shortens latter stage of ripening.
Description
Technical field
The present invention relates to modern Chinese herbal medicine cultivation technology field, more particularly to a kind of raising Panax notoginseng seeds germination rate and shortening
The hormone treatment method of latter stage of ripening.
Background technology
Radix Notoginseng (Panax notoginseng (Burk.) F.H.Chen) be Araliaceae (Araliaceae) Panax for many years
Raw herbaceous plant, is a kind of rare Chinese medicine, and rhizome contains the active constituent there are many five note saponin(e of dammarane type Fourth Ring, tool
Have a hemostasis, the anti-heart, brain, hepatic ischemia treat coronary heart disease, anti-inflammatory, removing toxic substances, reduce blood pressure and blood lipoid the effect of.
Radix Notoginseng is most characteristic one of the traditional Chinese medicinal materials assortment in Yunnan Province, and Genuine producing area is in Wenshan Prefecture of Yunnan Province and its circumferentially
Area, Yunnan Province's notoginseng planting area and yield account for 95% or more of the whole nation.Currently, Radix Notoginseng is most widely used effective breeding
Mode is seminal propagation, however, Panax notoginseng seeds are a kind of typical Recalcitrant Seeds, and has Morphology And Physiology suspend mode, after-ripening
Phase is 45~60 days.It sprouts under field conditions (factors), is unfavorable for asking for agricultural production practice there are latter stage of ripening is long, germination rate is low etc.
Topic.
Invention content
To solve the problems, such as that Panax notoginseng seeds natural conditions lower latter stage of ripening is long, it is slow to sprout, the present invention provides a kind of raisings
Panax notoginseng seeds germination rate and the hormone treatment method for shortening latter stage of ripening.
The present invention above-mentioned technical purpose technical scheme is that：
A kind of hormone treatment method for improving Panax notoginseng seeds germination rate and shortening latter stage of ripening, includes the following steps：
S1, Seeds preprocess：Clear water is cleaned after seed peeling, with germicidal solution soaking disinfection, then is washed twice, then room
It inside dries in the shade to obtain clean seed；
S2, seed soaking：The clean seed obtained through S1 step process is the red mould of 500mg/L with mass concentration in container
Plain seed soaking solution carries out seed soaking for 24 hours, and the volume ratio of seed and seed soaking solution is 1:3~4；Then it is cleaned twice with clear water
Seed after seed soaking dries to obtain clean seed in interior；
S3, husky Stratificated treatment：Clean seed that S2 steps are obtained and moistening river sand by volume 1:5 mix thoroughly, are placed in
In the good full mesh basket of gas, sand bed product interior is protected from light preservation 50~60 days at 4~15 DEG C.
The further setting of the present invention is：The seed of the S1 steps is removed the peel, and is removed red fruit skin using artificial manually rub with the hands, is avoided use
Mechanical treatment, seed caused by avoiding damage.
The further setting of the present invention is：Germicidal solution in the S1 steps chooses 5%CuSO4Solution, bactericidal liquid leaching
Bubble disinfecting time is 30min.
The further setting of the present invention is：Seed sterilized in the S1 steps, after washing twice, on indoor filter paper
It dries in the shade, the moisture that dries in the shade gently wipes the surface of the seed no-watermark using filter paper as standard.
The further setting of the present invention is：Container in the S2 steps uses sealed membrane sealing treatment, and avoid light place
In the environment that temperature is 4-6 DEG C.
The further setting of the present invention is：River sand grain size in the S3 steps is in 1-3mm, using preceding through high-temperature sterilization；
The whole process river sand humidity of husky Stratificated treatment is 23-27%.
The further setting of the present invention is：A seed quickly was stirred every 15 days in S3, sand bed product processing procedure, is made
Seed breathes freely and obtains enough oxygen.
In conclusion the method for the present invention is easy to operate, is easy to grasp, Panax notoginseng seeds latter stage of ripening length can be solved very well, is sprouted
The problems such as rate is low not only improves the germination rate of Panax notoginseng seeds, also breaking dormancy in advance, shortens latter stage of ripening, substantially increase it
Germination efficiency.
Description of the drawings
Fig. 1 is the situation of change of embryo rate in Panax notoginseng seeds After-ripening in the embodiment of the present invention；
Panax notoginseng seeds complete the sub-cases of after-ripening and sprouting when Fig. 2 is lamination 50 days in the embodiment of the present invention；
Fig. 3 is Panax notoginseng seeds sprouting situation of the lamination after 50 days in the embodiment of the present invention.
Specific implementation mode
Below in conjunction with attached drawing, invention is further described in detail.
Specific embodiment is only explanation of the invention, is not limitation of the present invention, those skilled in the art
The modification that not creative contribution can be made to the present embodiment as needed after reading this specification, but as long as in this hair
It is all protected by Patent Law in bright right.
A kind of embodiment, hormone treatment method for improving Panax notoginseng seeds germination rate and shortening latter stage of ripening, includes the following steps：
(1) seed collection：It is picked up from the Radix Notoginseng proving ground of Wenshan Prefecture of Yunnan Province with Panax notoginseng seeds, chooses triennial plant
Ripe, full seed is spare.
(2) Seeds preprocess：After seed red fruit skin is removed in artificial stranding, 5%CuSO is used4Solution soaking disinfection 30min, with distillation
Water rinses seed well, is laid on filter paper and dries in the shade surface moisture in interior, the moisture that dries in the shade gently wiped with filter paper the surface of the seed without
Watermark is standard.
(3) HORMONE TREATMENT：CuSO will be passed through4The seed seed soaking solution of solution disinfection processing is 1 by volume:3.5 into
Row impregnates, and the container ParafilmTM of immersion is placed in 4-6 degrees Celsius of environment, keeps for 24 hours；The seed soaking solution quality
The Gibberellins solution of a concentration of 500mg/L.It impregnates and terminates then to clean seed twice with clear water, dry to obtain clean kind in interior
Son pays attention to dry seed too long, otherwise seed may be caused to lose vigor.
(4) husky Stratificated treatment：Suitable netted plastic basket with holes is chosen, one layer of wet river through high-temperature sterilization is spread at basket bottom
Sand, then will be 1 by volume:5 mixing are placed in plastic crate through step (3) treated Panax notoginseng seeds and wet river sand mixture
Interior, then mixture is filled up with wet sand again away from basket 3~4cm of mouth, and plastic crate is then placed on the shady and cool indoor progress being protected from light
Lamination preservation, at 4~15 DEG C, the time is 60 days for indoor temperature control, and it is primary quickly to stir seed every 15 days；Pay attention to entire
Keep the water content of wet sand always 25% or so in Stratification time.
Correlation test：
Experimental group：According to step process described in embodiment；
Control group (CK)：In addition to using clear water to replace the gibberellin (GA of 500mg/L3) other than solution impregnated, remaining
Step is identical as experimental group.
Embryo rate measures：Panax notoginseng seeds are longitudinal sectional along embryo, it is placed in electronic stereomicroscope (SteREODiscoveryV.12, moral
State's Zeiss) under observe plumule, the endosperm development situation in different after-ripening periods, take pictures and measure its length, the measurement of plumule, endosperm
Use ratio ruler calculating method.The measurement of embryo rate is independently repeated 20 times (n=20).
Embryo rate (Em/En)=embryo length/endosperm length × 100%
Germination rate measures：It chooses through 50 days husky Stratificated treatments, not yet sprout, Panax notoginseng seeds in the same size, health is full,
1% hypochlorite disinfectant is handled, and is placed in the culture dish containing wet sand (per 50, ware), is cultivated, cultivate under Indoor Natural illumination
20 DEG C or so of temperature.After planting next day records germination seed number, until the 9th day, and calculates germination rate every three days.
Germination rate=germination seed number/sowing number × 100%
Experimental record result as shown in attached drawing 1, attached drawing 2, attached drawing 3,
As shown in Figure 1, in gibberellin (GA3) concentration be respectively 500mg/L seed soakings in clear water control group, first 30 days
GA3500mg/L is similar to control group embryonic development trend, GA after 30 days3500mg/L processing group embryo rates significantly increase, at 60 days
GA3500mg/L processing group embryo rates are significantly higher than control group, GA3The embryo rate of 500mg/L processing groups reaches 80.7 ± 7.6%, right
63.6 ± 7.5% are only reached according to a group embryo rate.
When observing 35 days in experimentation, GA3There is kind of a skin cracking phenomena in 500mg/L processing groups, at 40 days
There is seed to start to sprout radicle, and there has been no kind of a skin cracking phenomenas for control group.Fig. 2 it is found that 50 days whens observe GA3
Have a large amount of seeds in 500mg/L processing groups to sprout, radicle length is apparently higher than control group.And Stratificated treatment is after 50 days, by Fig. 3
It is found that GA3500mg/L processing group germination rates are consistently higher than control group, shift to an earlier date the seed number of breaking dormancy and to be apparently higher than pair
According to group.
Claims (7)
1. a kind of hormone treatment method for improving Panax notoginseng seeds germination rate and shortening latter stage of ripening, includes the following steps：
S1, Seeds preprocess：Clear water is cleaned after seed peeling, with germicidal solution soaking disinfection, is washed with water twice, then indoor
It dries in the shade to obtain clean seed；
S2, seed soaking：The clean seed obtained through S1 step process, in container, the gibberellin for being 500mg/L with mass concentration soaks
Kind solution carries out seed soaking for 24 hours, and the volume ratio of seed and seed soaking solution is 1:3~4；Then it is cleaned with clear water and is soaked seed twice
Treated seed, dries to obtain clean seed in interior；
S3, husky Stratificated treatment：Clean seed that S2 steps are obtained and moistening river sand by volume 1:5 mix thoroughly, are placed in ventilative good
In good full mesh basket, sand bed product interior is protected from light preservation 50~60 days at 4~15 DEG C.
2. a kind of hormone treatment method for improving Panax notoginseng seeds germination rate and shortening latter stage of ripening according to claim 1,
It is characterized in that：The seeds of the S1 steps is removed the peel, and red fruit skin is removed using artificial manually rub with the hands, is avoided with mechanical treatment, caused by avoiding
Seed damages.
3. a kind of hormone treatment method for improving Panax notoginseng seeds germination rate and shortening latter stage of ripening according to claim 1,
It is characterized in that：Germicidal solution in the S1 steps chooses 5%CuSO4Solution, bactericidal liquid soaking disinfection time are 30min.
4. a kind of hormone treatment method for improving Panax notoginseng seeds germination rate and shortening latter stage of ripening according to claim 1,
It is characterized in that：Seed sterilized in the S1 steps, after washing twice, dries in the shade, the moisture that dries in the shade is with filter paper on indoor filter paper
Light the surface of the seed no-watermark of wiping is standard.
5. a kind of hormone treatment method for improving Panax notoginseng seeds germination rate and shortening latter stage of ripening according to claim 1,
It is characterized in that：Container in the S2 steps uses sealed membrane sealing treatment, and the environment that avoid light place is 4-6 DEG C in temperature
In.
6. a kind of hormone treatment method for improving Panax notoginseng seeds germination rate and shortening latter stage of ripening according to claim 1,
It is characterized in that：River sand grain size in the S3 steps is in 1-3mm, using preceding through high-temperature sterilization；The whole process of husky Stratificated treatment
River sand humidity is 23-27%.
7. the hormone treatment method of a kind of raising Panax notoginseng seeds germination rate and shortening latter stage of ripening according to claim 1-6,
It is characterized in that：A seed quickly was stirred every 15 days in S3, sand bed product processing procedure, so that seed is breathed freely and obtains enough
Oxygen.
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| CN201810417580.2A CN108718594A (en) | 2018-05-04 | 2018-05-04 | A kind of hormone treatment method for improving Panax notoginseng seeds germination rate and shortening latter stage of ripening |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110574521A (en) * | 2019-08-13 | 2019-12-17 | 云南农业大学 | Hormone treatment method for prolonging post-maturation period of pseudo-ginseng seeds |
| CN116267090A (en) * | 2023-05-22 | 2023-06-23 | 云南泽润三七种植有限公司 | Method for improving germination rate of pseudo-ginseng seeds |
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| US20150070492A1 (en) * | 2013-09-09 | 2015-03-12 | Jamco Corporation | Safety monitoring system of galley |
| CN107278412A (en) * | 2017-06-16 | 2017-10-24 | 云南中医学院 | A kind of breeding method for improving Panax stipuleanatus Tsai et Feng germination rate |
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- 2018-05-04 CN CN201810417580.2A patent/CN108718594A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150070492A1 (en) * | 2013-09-09 | 2015-03-12 | Jamco Corporation | Safety monitoring system of galley |
| CN107278412A (en) * | 2017-06-16 | 2017-10-24 | 云南中医学院 | A kind of breeding method for improving Panax stipuleanatus Tsai et Feng germination rate |
Non-Patent Citations (2)
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| 杨凯等: "三七种子后熟过程种胚发育和6种内源激素的动态变化", 《中药材》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110574521A (en) * | 2019-08-13 | 2019-12-17 | 云南农业大学 | Hormone treatment method for prolonging post-maturation period of pseudo-ginseng seeds |
| CN116267090A (en) * | 2023-05-22 | 2023-06-23 | 云南泽润三七种植有限公司 | Method for improving germination rate of pseudo-ginseng seeds |
| CN116267090B (en) * | 2023-05-22 | 2023-08-01 | 云南泽润三七种植有限公司 | Method for improving germination rate of pseudo-ginseng seeds |
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