CN107278412A - A kind of breeding method for improving Panax stipuleanatus Tsai et Feng germination rate - Google Patents
A kind of breeding method for improving Panax stipuleanatus Tsai et Feng germination rate Download PDFInfo
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- CN107278412A CN107278412A CN201710458344.0A CN201710458344A CN107278412A CN 107278412 A CN107278412 A CN 107278412A CN 201710458344 A CN201710458344 A CN 201710458344A CN 107278412 A CN107278412 A CN 107278412A
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- panax stipuleanatus
- stipuleanatus tsai
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Abstract
The invention discloses a kind of breeding method for improving Panax stipuleanatus Tsai et Feng germination rate, comprise the following steps：Panax stipuleanatus Tsai et Feng seed full, of the same size is selected, Stratificated treatment is carried out with damp sand, treats that seed, by after-ripening, is cleaned with clear water, blots the surface of the seed moisture；Soaked seed with salicylic acid, distilled water flushing is used 2~3 times after seed immersion；Seed after bigcatkin willow acid treatment is put in the flowerpot for filling soil, each 50 seeds of flowerpot are repeated 3 times, are placed in light culture in constant incubator, divulge information 5min daily.The present invention can improve the germination percentage and germinating energy of Panax stipuleanatus Tsai et Feng, can be with large scale cultivating Panax stipuleanatus Tsai et Feng seedling.All there is huge potential value in terms of recurrence, Emulational culture in rare and endangered medicinal plant Panax stipuleanatus Tsai et Feng.
Description
Technical field
The invention belongs to technical field of plant cultivation, specifically, it is related to a kind of cultivation for improving Panax stipuleanatus Tsai et Feng germination rate
Method.
Background technology
Panax stipuleanatus Tsai et Feng (Panax stipuleanatus H.T.Tsai et K.M.Feng) is araliaceae ginseng plant,
Originate in southeastern Yunnan Honghe screen side, Wenshan Prefecture Maguan, Malipo, 1100~1700m of height above sea level mountain valleys humidity woods, be country
Second class protection plant.The old street of North Vietnam is also distributed, and in recent years, Shuicheng, Guizhou Province is also reported and is distributed.
Panax stipuleanatus Tsai et Feng is used as medicine with root, also known as wild pseudo-ginseng (Maguan, Malipo), perfume thorn (screen side), Sedum uizoon (Maguan), ring
7th, Herba Rhodiolae Henryi.Its root has the effects such as scattered stasis pain, hemostasis of curing the wound, nourishing, and rhizome is rich in saponin.Traumatic injury, wind can be treated
Wet pain, hemoptysis, traumatism and bleeding, haematemesis, bleeding from five sense organs or subcutaneous tissue, have blood in stool, uterine bleeding and eak after being ill, anaemia and pulmonary tuberculosis etc., Panax stipuleanatus Tsai et Feng is also
With stronger active anticancer.
The distribution relatively narrower of Panax stipuleanatus Tsai et Feng, stock number is less, dissipates the stasis of blood, analgesic therapy while Panax stipuleanatus Tsai et Feng has, cures the wound, only
Blood and the effect of nourishing.In order to meet medical needs, Panax stipuleanatus Tsai et Feng commerial growing is foreseeable.Under Panax stipuleanatus Tsai et Feng natural conditions
Seed germination rate is low, improves the germination rate of seed, will solve most basic link for the cultivation of Panax stipuleanatus Tsai et Feng.
The content of the invention
In view of this, there is provided a kind of breeding method for improving Panax stipuleanatus Tsai et Feng germination rate for the problem of present invention is directed to above-mentioned.
In order to solve the above-mentioned technical problem, the invention discloses a kind of breeding method for improving Panax stipuleanatus Tsai et Feng germination rate, bag
Include following steps：
Step 1, Panax stipuleanatus Tsai et Feng seed full, of the same size is selected, Stratificated treatment is carried out with damp sand, after seed after
It is ripe, cleaned with clear water, blot the surface of the seed moisture；
Step 2, soaked seed with salicylic acid, with distilled water flushing 2~3 times after seed immersion；
Step 3, the seed after bigcatkin willow acid treatment is put in the flowerpot for filling soil, each 50 seeds of flowerpot, repeats 3
It is secondary, light culture in constant incubator is placed in, divulge information 5min daily.
Further, lamination treatment temperature is 20 DEG C in step 1, and the water content of damp sand is 20%.
Further, salicylic concentration is 1-300mg/L in step 2, and seed soaking time is 24h.
Further, the cultivation temperature of constant incubator is 10~30 DEG C, the daily illumination 8h of light culture, light intensity in step 3
For 1250lx.
Further, soil conservation humidity 25%~30% in step 3, the humidity of constant incubator keeps 70%~
85%.
Compared with prior art, the present invention can be obtained including following technique effect：
1) with the salicylic acid SA processing Panax stipuleanatus Tsai et Feng seeds of various concentrations, compared with untreated seed, germination percentage and hair
Bud gesture all increases, and most suitable mass concentration is 100mg/LSA, is conducive to cultivating Panax stipuleanatus Tsai et Feng strong sprout.
2) method of the invention is simple to operate, and required reagent is common, can reduce the production in Panax stipuleanatus Tsai et Feng cultivation
Cost.
3) present invention can improve the germination percentage and germinating energy of Panax stipuleanatus Tsai et Feng, can be with large scale cultivating Panax stipuleanatus Tsai et Feng seedling.
All there is huge potential value in terms of the recurrence of rare and endangered medicinal plant Panax stipuleanatus Tsai et Feng, Emulational culture.
Certainly, any product for implementing the present invention it is not absolutely required to while reaching all the above technique effect.
Embodiment
Describe embodiments of the present invention in detail below in conjunction with embodiment, thereby to the present invention how application technology hand
Section can fully understand and implement according to this to solve technical problem and reach the implementation process of technology effect.
The invention discloses a kind of breeding method for improving Panax stipuleanatus Tsai et Feng germination rate, comprise the following steps：
Step 1, Panax stipuleanatus Tsai et Feng seed full, of the same size is selected, be under the conditions of 20 DEG C, to be with water content in temperature
20% damp sand carries out Stratificated treatment, treats that seed, by after-ripening, is cleaned with clear water, blots the surface of the seed moisture；
Step 2, the salicylic acid for being 1-300mg/L with concentration seed soaking 24h, distilled water flushing is used 2~3 times after seed immersion；
Step 3, the seed after bigcatkin willow acid treatment is put in the flowerpot for filling soil respectively, each 50 seeds of flowerpot,
It is repeated 3 times, is placed in light culture in 10~30 DEG C of constant incubators, daily illumination 8h, light intensity is that the soil in 1250lx, flowerpot is protected
Humidity 25%~30% is held, the humidity of incubator keeps 70%~85%, and divulge information 5min daily.Every morning, observation in afternoon note
Carry germination.Germination calculates germination percentage, Germination energy index after terminating, and the stem for determining seedling is thick, plant height, radicle length, root/shoot ratio
Etc. index.
The optimal germination temperature of Panax stipuleanatus Tsai et Feng seed is 20 DEG C；Soil optimum humidity need to remain 30%；Air optimal wet
Spend for 85%.Soil moisture is less than 20%, germination is influenceed, higher than 40%, it may occur that root rot and death.
Embodiment 1
A kind of breeding method for improving Panax stipuleanatus Tsai et Feng germination rate, comprises the following steps：
Step 1, Panax stipuleanatus Tsai et Feng seed full, of the same size is selected, be under the conditions of 20 DEG C, to be with water content in temperature
20% damp sand carries out Stratificated treatment, treats that seed, by after-ripening, is cleaned with clear water, blots the surface of the seed moisture；
Step 2, with concentration be respectively 0,50,100,200,300mg/L salicylic acid seed soaking 24h, with steaming after seed immersion
Distilled water is rinsed 2~3 times；
Step 3, the seed after bigcatkin willow acid treatment is put in the flowerpot for filling soil respectively, each 50 seeds of flowerpot,
It is repeated 3 times, is placed in light culture in 20 DEG C of constant incubators, daily illumination 8h, light intensity is that the soil conservation in 1250lx, flowerpot is wet
Degree 30%, the humidity of incubator keeps 85%, and divulge information 5min daily.Germination is recorded in every morning, observation in afternoon.Germination knot
Germination percentage, Germination energy index are calculated after beam, the indexs such as stem thick, plant height, radicle length, the root/shoot ratio of seedling are determined.
Embodiment 2
A kind of breeding method for improving Panax stipuleanatus Tsai et Feng germination rate, comprises the following steps：
Step 1, Panax stipuleanatus Tsai et Feng seed full, of the same size is selected, be under the conditions of 20 DEG C, to be with water content in temperature
20% damp sand carries out Stratificated treatment, treats that seed, by after-ripening, is cleaned with clear water, blots the surface of the seed moisture；
Step 2, be 0 with concentration, 50,100,200,300mg/L salicylic acid soak seed 24h, use distilled water after seed immersion
Rinse 2~3 times；
Step 3, the seed after bigcatkin willow acid treatment is put in the flowerpot for filling soil respectively, each 50 seeds of flowerpot,
It is repeated 3 times, is placed in light culture in 10 DEG C of constant incubators, daily illumination 8h, light intensity is that the soil conservation in 1250lx, flowerpot is wet
Degree 25%, the humidity of incubator keeps 70%, and divulge information 5min daily.Germination is recorded in every morning, observation in afternoon.Germination knot
Germination percentage, Germination energy index are calculated after beam, the indexs such as stem thick, plant height, radicle length, the root/shoot ratio of seedling are determined.
Embodiment 3
A kind of breeding method for improving Panax stipuleanatus Tsai et Feng germination rate, comprises the following steps：
Step 1, Panax stipuleanatus Tsai et Feng seed full, of the same size is selected, be under the conditions of 20 DEG C, to be with water content in temperature
20% damp sand carries out Stratificated treatment, treats that seed, by after-ripening, is cleaned with clear water, blots the surface of the seed moisture；
Step 2, with concentration be respectively 0,50,100,200,300mg/L salicylic acid seed soaking 24h, with steaming after seed immersion
Distilled water is rinsed 2~3 times；
Step 3, the seed after bigcatkin willow acid treatment is put in the flowerpot for filling soil respectively, each 50 seeds of flowerpot,
It is repeated 3 times, is placed in light culture in 30 DEG C of constant incubators, daily illumination 8h, light intensity is that the soil conservation in 1250lx, flowerpot is wet
Degree 25%~30%, the humidity of incubator keeps 70%~85%, and divulge information 5min daily.Hair is recorded in every morning, observation in afternoon
Bud situation.Germination calculates germination percentage, Germination energy index after terminating, and determines stem thick, plant height, radicle length, root/shoot ratio of seedling etc. and refers to
Mark.
Embodiment 4
A kind of breeding method for improving Panax stipuleanatus Tsai et Feng germination rate, comprises the following steps：
Step 1, Panax stipuleanatus Tsai et Feng seed full, of the same size is selected, be under the conditions of 20 DEG C, to be with water content in temperature
20% damp sand carries out Stratificated treatment, treats that seed, by after-ripening, is cleaned with clear water, blots the surface of the seed moisture；
Step 2, with concentration be respectively 1,50,100,200,300mg/L salicylic acid seed soaking 24h, with steaming after seed immersion
Distilled water is rinsed 2~3 times；
Step 3, the seed after bigcatkin willow acid treatment is put in the flowerpot for filling soil respectively, each 50 seeds of flowerpot,
It is repeated 3 times, is placed in light culture in 15 DEG C of constant incubators, daily illumination 8h, light intensity is that the soil conservation in 1250lx, flowerpot is wet
Degree 28%, the humidity of incubator keeps 80%, and divulge information 5min daily.Germination is recorded in every morning, observation in afternoon.Germination knot
Germination percentage, Germination energy index are calculated after beam, the indexs such as stem thick, plant height, radicle length, the root/shoot ratio of seedling are determined.
Comparative example 1
Tested with the conventional hormones gibberellins GA for breaking plant dormancy, equally select screen full, of the same size
Side Panax notoginseng seeds, carry out Stratificated treatment, treat that seed, by after-ripening, is cleaned with clear water, blots the surface of the seed moisture, then use gibberellin
GA seed soaking 24h.Gibberellin GA sets 0,50,100,200,300mg/L5 mass concentration processing.Rushed after seed immersion with distilled water
Wash 2~3 times, be individually placed to be covered with the germination dish of 2 metafiltration paper, per ware 50 seeds, be repeated 3 times, be placed in 20 DEG C it is incubated
Light culture in case, daily illumination 8h, light intensity is 1250lx, and culture dish keeps occurring without moisture film around moistening and seed, logical daily
Wind 5min.Germination is recorded in every morning, observation in afternoon.Germination calculates germination percentage, Germination energy index after terminating, and determines seedling
The index such as stem thick, plant height, radicle length, root/shoot ratio, experimental result is shown in Table 1.
The different quality concentration salicylic acid of table 1 and gibberellin seed soaking are sprouted to Panax stipuleanatus Tsai et Feng seed, the influence of seedling state
As it can be seen from table 1 being above with Panax stipuleanatus Tsai et Feng percentage of seedgermination, the Germination energy index of salicylic acid SA seed soakings
Compare, but its raising to germination rate becomes apparent compared with vigor, illustrates that with SA seed soaking the germination rate of seed can be significantly improved.
Panax stipuleanatus Tsai et Feng seed treated SA is in germination process, and the radicle growth of seed is more vigorous, shows as radicle elongation speed
Comparatively fast, root hair is more.Within the specific limits, with the rise of SA concentration, radicle growth speed is accelerated, and root hair increases.Compare suitable
Suitable seed soaking concentration is 100mg/LSA, and its vigor can improve 20.3%, and germination rate can improve 26.9%.SA seed soaking is conducive to
Stem thickening, root increases, and improves root/shoot ratio, is significantly higher than control after SA (100mg/LSA) processing through certain mass concentration, and high
The Panax stipuleanatus Tsai et Feng seed seedling state of mass concentration SA processing decreases.It can be seen that appropriate mass concentration 100mg/LSA seed soaking has
Beneficial to cultivation Panax stipuleanatus Tsai et Feng strong sprout.
Vigor can be significantly improved with the gibberellin GA Panax stipuleanatus Tsai et Feng seeds handled, maximum is compareed with blank group to be carried
It is high by 30.9%, but the raising of germination rate is seemed not substantially, seedling state illustrates only be improved with GA seed soaking without obvious change
The vigor of Panax stipuleanatus Tsai et Feng seed, wherein convenient processing is 200mg/L processing.
From the foregoing, it will be observed that handling Panax stipuleanatus Tsai et Feng seed with the salicylic acid SA of various concentrations, compared with untreated seed, germinateed
Rate and germinating energy all increase, and most suitable mass concentration is 100mg/LSA, are conducive to cultivating Panax stipuleanatus Tsai et Feng strong sprout.And day
The conventional gibberellin GA of breaking dormancy, can only improve the vigor of Panax stipuleanatus Tsai et Feng seed in normal agricultural production.On screen side three
In seven cultivation, the plant hormone of seed seedling-raising strong sprout optimal selection is salicylic acid SA, and optimum concentration is 100mg/L.
Some preferred embodiments of invention have shown and described in described above, but as previously described, it should be understood that invention is not
Form disclosed herein is confined to, the exclusion to other embodiment is not to be taken as, and available for various other combinations, modification
And environment, and can be carried out in invention contemplated scope described herein by the technology or knowledge of above-mentioned teaching or association area
Change., then all should be in the appended power of invention and the change and change that those skilled in the art are carried out do not depart from the spirit and scope of invention
In the protection domain that profit is required.
Claims (5)
1. a kind of breeding method for improving Panax stipuleanatus Tsai et Feng germination rate, it is characterised in that comprise the following steps：
Step 1, Panax stipuleanatus Tsai et Feng seed full, of the same size is selected, carries out Stratificated treatment with damp sand, treat seed by after-ripening,
Cleaned with clear water, blot the surface of the seed moisture；
Step 2, soaked seed with salicylic acid, with distilled water flushing 2~3 times after seed immersion；
Step 3, the seed after bigcatkin willow acid treatment is put in the flowerpot for filling soil, each 50 seeds of flowerpot are repeated 3 times,
Light culture in constant incubator is placed in, divulge information 5min daily.
2. the breeding method according to claim 1 for improving Panax stipuleanatus Tsai et Feng germination rate, it is characterised in that lamination in step 1
Treatment temperature is 20 DEG C, and the water content of damp sand is 20%.
3. the breeding method according to claim 1 for improving Panax stipuleanatus Tsai et Feng germination rate, it is characterised in that bigcatkin willow in step 2
The concentration of acid is 1-300mg/L, and seed soaking time is 24h.
4. the breeding method according to claim 1 for improving Panax stipuleanatus Tsai et Feng germination rate, it is characterised in that constant temperature in step 3
The cultivation temperature of incubator is 10~30 DEG C, the daily illumination 8h of light culture, and light intensity is 1250lx.
5. the breeding method according to claim 1 for improving Panax stipuleanatus Tsai et Feng germination rate, it is characterised in that soil in step 3
Humidity 25%~30% is kept, the humidity of constant incubator keeps 70%~85%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108718594A (en) * | 2018-05-04 | 2018-11-02 | 云南农业大学 | A kind of hormone treatment method for improving Panax notoginseng seeds germination rate and shortening latter stage of ripening |
-
2017
- 2017-06-16 CN CN201710458344.0A patent/CN107278412A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108718594A (en) * | 2018-05-04 | 2018-11-02 | 云南农业大学 | A kind of hormone treatment method for improving Panax notoginseng seeds germination rate and shortening latter stage of ripening |
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Application publication date: 20171024 |