CN103782785B - A kind of plum blossom seeds cultivation method - Google Patents
A kind of plum blossom seeds cultivation method Download PDFInfo
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- CN103782785B CN103782785B CN201410061724.7A CN201410061724A CN103782785B CN 103782785 B CN103782785 B CN 103782785B CN 201410061724 A CN201410061724 A CN 201410061724A CN 103782785 B CN103782785 B CN 103782785B
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- plum blossom
- secundine
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Abstract
The invention discloses a kind of plum blossom seeds cultivation method, it comprises the steps such as seed pre-treatment, Dormancy breaking, cultivation germination, seedling replanting, and wherein the method for Dormancy breaking seed is divested secundine and the Gibberellins solution being placed in clear water or 100 ~ 400mg/L is soaked 1 day in 22 DEG C.Traditional plum blossom seeds cultivation method to need before cultivation Cord blood more than 2 months to break the resting stage of plum blossom seed, and the present invention can obtain plum blossom health seedling in 2 weeks, compared with prior art cultivated more rapidly and efficiently.The germination rate of plum blossom seed can also be brought up to more than 80% by the present invention, and relative to the 30-40% of existing cultivation method, germination rate improves nearly 1 times.
Description
Technical field
The invention belongs to ornamental plant technical field of cultivation, be specifically related to a kind of plum blossom seeds cultivation method.
Background technology
Plum blossom (Prunus mume Sieb.et Zucc) is one of Chinese ten great tradition famous flowers, and plum blossom seed has the characteristic of deep dormancy, usually needs more than 4 DEG C of low-temperature preservations, 2 first quarter moons with breaking dormancy before cultivation.This planting type is consuming time longer, in long-term preserving process, seed easy infection mould and rotten disorderly, cause planting percent low.Particularly, plum blossom seed germination rate after above-mentioned process is not high, as " snow plum " is only 39.1% with hybrid seed germination rate after above-mentioned process of " bean sheet jelly palace powder ".Plum blossom inter breed crossing workload is large, and ripening rate is low, and the acquisition of hybrid seed is hard-earned, and germination rate low be the bottleneck problem of restriction plum blossom crossbreeding.
Summary of the invention
The present invention is intended to set up a kind of new plum blossom seeds cultivation method, and the method can not only break the plum blossom seed dormancy phase fast, and can improve the germination rate of plum blossom seed.
Above-mentioned purpose is achieved through the following technical solutions:
A kind of plum blossom seeds cultivation method, comprises with following step:
1) seed pre-treatment: get plum blossom ripening fruits, removing pericarp and exosper, takes out seed and with 70% alcohol-pickled 30 seconds, sterile distilled water rinsed twice sterilization;
2) Dormancy breaking: divest secundine gently with the tweezers of having sterilized, do not damage plumule, the Gibberellins solution seed divesting secundine being placed in clear water or 100 ~ 400mg/L is soaked 1 day in 22 DEG C;
3) germination is cultivated: from clear water or Gibberellins solution, take out seed, rinse once with sterile distilled water, then be placed in and cultivate box, doubly adding damp sand and the damp sand making to cover above seed 0.5-2 centimetre to cultivating in box by the 3-8 of seed volume, being positioned in the environment of 22 DEG C and cultivating until grow seedling;
4) seedling replanting: treat that seedling grows tall to about 15cm, is with sand by seedling replanting in soil.
Preferably, the concentration of described Gibberellins solution is 150 ~ 250mg/L.
Preferably, in step 3), in cultivation box, add damp sand by 5 times of seed volume, make the damp sand covering 1 centimetre above seed.
Preferably, containing percentage by weight in described damp sand is the rice chaff of 0.5 ~ 5% and the carbendazim of 0.001 ~ 0.005%.
Further preferably, containing percentage by weight in described damp sand is the rice chaff of 1% and carbendazim (N-(2-benzimidazole the base)-methyl carbamate of 0.003%).
The invention has the beneficial effects as follows:
1) traditional plum blossom seeds cultivation method to need before cultivation Cord blood more than 2 months to break the resting stage of plum blossom seed, and the present invention can obtain plum blossom health seedling in 2 weeks, compared with prior art cultivated more rapidly and efficiently.
2) germination rate of plum blossom seed can be brought up to more than 80% by the present invention, and relative to the 30-40% of existing cultivation method, germination rate improves nearly 1 times.
3) the present invention can make plum blossom seed sprouting in very short time, considerably reduces the chance that seed is infected by damage by disease and insect, basic free of losses after seedling replanting.
4) the present invention is simple to operate, with low cost, is easy to promote.
Accompanying drawing explanation
Fig. 1 is the structural representation of plum blossom fruit.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1 secundine and soak are on the impact of plum blossom seed dormancy and germination
The ripening fruits of random selecting plum blossom " snow plum ", removing pericarp and exosper, take out seed and with 70% alcohol-pickled 30 seconds, sterile distilled water rinsed twice sterilization, then uses the tweezers of having sterilized to divest secundine gently.The plum blossom seed divesting secundine is soaked 1 day (22 DEG C) with clear water, plum blossom secundine leaching liquor respectively, take out seed, rinse once with sterile distilled water, then be placed in and cultivate box, in cultivation box, add damp sand by 5 times of seed volume, make the damp sand of kind of subcovering 1 centimetre, be positioned in the environment of 22 DEG C and cultivate, after 4 days, namely there is seed to start to sprout, when 12 days, add up germination rate (chitting piece number/confession test seed sum × 100%).
Contrast (not divesting secundine): get plum blossom ripening fruits, removing pericarp and exosper, soak with clear water after sterilization, cultivate and germinate, statistics germination rate.Each process seed number used is 30 above, repeats twice.
Plum blossom secundine leaching liquor used is prepared from by the following method: get 20 plum blossom seeds by the secundine divested, clay into power with liquid nitrogen, proceed to test tube, 95% ethanolic solution 4 DEG C adding 5ml soaks 24 hours, then centrifugal, Aspirate supernatant 4ml, evaporates 12 hours, is then settled to 100ml with sterile purified water.
Result of the test: the plum blossom percentage of seedgermination of each processed group is in table 1.
Table 1 secundine is on the impact of plum blossom seed dormancy and germination
| Processed group | Germination rate (mean+SD %) |
| Remove secundine+clear water | 84.5±3 |
| Remove secundine+leaching liquor | 39.0±1 |
| Do not remove secundine+clear water | 11.0±1 |
1) as shown in table 1: only to remove the plum blossom seed after endocarp and exosper, germination rate after clear water soaks is 11.0%, and remove the plum blossom seed after secundine, after soaking with clear water, germination rate is up to 84.5%, extremely remarkable with the germination rate difference of the plum blossom seed of band secundine.
2) plum blossom secundine leaching liquor significantly suppresses the germination of secundine plum blossom seed (germination rate is 39%), and the germination rate of secundine plum blossom seed that goes that clear water soaks is 84.5%, and both differences are extremely remarkable.
Above-mentioned test illustrates: may contain Germination Inhibitors in plum blossom seed secundine, and Germination Inhibitors causes the seed dormant key factor of plum blossom.
For verifying the above results further, then getting a variety of Chinese cabbage seed, using plum blossom secundine leaching liquor and aseptic distillation water soaking respectively, be then placed in the culture dish vernalization being placed with moistening filter paper, when 3 days, add up germination rate.Result is: plum blossom secundine leaching liquor significantly suppresses the sprouting of a variety of Chinese cabbage seed, and germination rate is 38.5%, and a variety of Chinese cabbage percentage of seedgermination of distilled water immersion is 51.0%.
Embodiment 2 gibberellin concentration is on the impact of removing secundine plum blossom percentage of seedgermination
According to the test method in embodiment 1, the plum blossom seed divesting secundine is soaked by the Gibberellins solution of clear water, variable concentrations respectively, and then cultivate germination, germination rate is added up when 12 days, and 1 month time, statistics planting percent (seedling strain number/confession test seed sum × 100%), the results are shown in Table 2.
Table 2 gibberellin concentration is on the impact of removing secundine plum blossom percentage of seedgermination and planting percent
Compared with contrasting with clear water, the Gibberellins solution of 250mg/L can improve the germination rate removing secundine plum blossom seed, when gibberellin concentration reaches 400mg/L, goes the germination rate of secundine plum blossom seed significantly to decline.The more important thing is, the seedling after the gibberellin process of 150mg/L and 250mg/L is more sturdy, and its planting percent all contrasts higher than clear water.
Embodiment 3
A kind of plum blossom seeds cultivation method, comprises with following step:
Get " snow plum " for maternal ripening fruits, removing pericarp and exosper, take out seed and with 70% alcohol-pickled 30 seconds, sterile distilled water rinsed twice sterilization; Then divest secundine (film quality seed coat) gently with the tweezers of having sterilized, do not damage plumule; Be placed in clear water by removing the seed of secundine to soak 24 hours in 22 DEG C; Seed is taken out from clear water, rinse once with sterile distilled water, then be placed in and cultivate box, in cultivation box, damp sand (be the rice chaff of 1% and the carbendazim of 0.003% containing accounting for damp sand percentage by weight) is added by 5 times of seed volume, make the damp sand covering 1 centimetre above seed, be positioned in the environment of 22 DEG C and cultivate until grow seedling; Treat that seedling grows tall to about 15cm, be with sand by seedling replanting in soil.Germination rate and planting percent reach more than 90%, and growth of seedling is healthy, and form is normal, and wherein, the cross hybrid seedling being transplanted to Hua Zhong Agriculture University's The Plum Garden is bloomed, and is playing its scientific research and ornamental value.
Claims (3)
1. a plum blossom seeds cultivation method, is characterized in that comprising with following step:
1) seed pre-treatment: get plum blossom ripening fruits, removing pericarp and exosper, takes out seed and with 70% alcohol-pickled 30 seconds, sterile distilled water rinsed twice sterilization;
2) Dormancy breaking: divest secundine gently with the tweezers of having sterilized, do not damage plumule, the Gibberellins solution seed divesting secundine being placed in 250mg/L is soaked 1 day in 22 DEG C;
3) germination is cultivated: from Gibberellins solution, take out seed, rinse once with sterile distilled water, then be placed in and cultivate box, doubly damp sand is added and the damp sand making to cover above seed 0.5-2 centimetre to cultivating in box by the 3-8 of seed volume, in described damp sand containing percentage by weight be 0.5 ~ 5% rice chaff and percentage by weight be the carbendazim of 0.001 ~ 0.005%, be positioned in the environment of 22 DEG C cultivate until grow seedling;
4) seedling replanting: treat that seedling grows tall to about 15cm, is with sand by seedling replanting in soil.
2. plum blossom seeds cultivation method according to claim 1, is characterized in that: step 3) in, in cultivation box, add damp sand by 5 times of seed volume, make the damp sand covering 1 centimetre above seed.
3. plum blossom seeds cultivation method according to claim 1, is characterized in that: containing percentage by weight in described damp sand is the rice chaff of 1% and the carbendazim of 0.003%.
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107278891B (en) * | 2017-06-22 | 2019-06-28 | 北京林业大学 | A kind of apricot plum quick breeding method for tissue culture |
| CN107258141B (en) * | 2017-07-05 | 2021-02-02 | 北京林业大学 | Plum blossom seed germination method |
| CN109006106A (en) * | 2018-06-29 | 2018-12-18 | 北京林业大学 | A kind of plum blossom seed seeding growing seedlings method |
| CN114793779B (en) * | 2022-05-27 | 2023-06-23 | 山西农业大学玉米研究所(山西省农业科学院玉米研究所) | Perilla seed high-yield planting and cultivating method |
| CN114982560B (en) * | 2022-06-17 | 2024-04-12 | 河北双星种业股份有限公司 | Method for raising seedlings by utilizing sunflower seeds |
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| CN102265747A (en) * | 2011-04-28 | 2011-12-07 | 梁文荣 | Two-stage seedling raising method using river sand as matrix |
| CN102308689A (en) * | 2011-06-27 | 2012-01-11 | 苏芹 | Technique for accelerating germination of flower seeds |
| CN103404268A (en) * | 2013-09-04 | 2013-11-27 | 华润三九医药股份有限公司 | Seedling culturing and planting method of holly root |
| CN103430760A (en) * | 2013-09-09 | 2013-12-11 | 北京林大林业科技股份有限公司 | Method for cultivating cold-proof flower and fruit dual-purpose plum |
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Patent Citations (4)
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|---|---|---|---|---|
| CN102265747A (en) * | 2011-04-28 | 2011-12-07 | 梁文荣 | Two-stage seedling raising method using river sand as matrix |
| CN102308689A (en) * | 2011-06-27 | 2012-01-11 | 苏芹 | Technique for accelerating germination of flower seeds |
| CN103404268A (en) * | 2013-09-04 | 2013-11-27 | 华润三九医药股份有限公司 | Seedling culturing and planting method of holly root |
| CN103430760A (en) * | 2013-09-09 | 2013-12-11 | 北京林大林业科技股份有限公司 | Method for cultivating cold-proof flower and fruit dual-purpose plum |
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| 不同处理对果梅种子萌发的影响;陈红等;《北方园艺》;20110915(第17期);第50页左栏第4段-第51页右栏第2段 * |
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