A kind of seed dormant method of blueberry of removing
Technical field
The present invention relates to a kind of plant propagation technical method, specifically relate to a kind of seed dormant method of blueberry of removing.
Background technology
Blueberry (Vaccinium Bracteatum Thunb.) has another name called south candle, dyes bean etc., is Ericaceae genus vaccinium evergreen shrubs or dungarunga, is distributed widely in south China various places; Blueberry is to integrate medicinal, the rare tree being worth such as keep healthy, view and admire, and exploitation prospect is wide.Blueberry has long applicating history in China, no matter is as food, or as medicine, all shows unique characteristics, and in annual April, has the custom of adopting its leaf and be cooked into black meal on the south of the River and other places; Although the distribution of blueberry is very wide; but due to the impact of human factor or bird pest insect pest; lack for a long time the effective protection to resource; blueberry resource is suffered extremely to destroy; gene resource is lost serious, and the original wild community in some areas even disappears, and therefore protects blueberry germ plasm resource; carry out the research of blueberry breeding and culturing, extremely urgent.Blueberry fruit maturation is atropurpureus berry, very easily suffers the harm of birds, insect etc., need to gather in time, and in its pulp, seed is tiny, and seed coat is hard, and seed has hibernation feature; Domestic existing scholar's research has been developed the germinating method of blueberry seed, and this method is different with method from the former mode, and rapid through the blueberry seed sprouting of this method processing, germination vigor is high, and germination rate can reach more than 95.3%; This method is thoroughly broken blueberry seed dormancy, has improved blueberry seminal propagation efficiency, has solved the bottleneck of blueberry seed Fast-propagation, to protection and the wild blueberry plant resources of exploitation, realizes blueberry artificial domesticating cultivation, significant.
Summary of the invention
Goal of the invention: in order to promote blueberry sowing to germinate rapidly, emerge neat, the object of the present invention is to provide a kind of simple to operate, with low cost, seed dormant method of releasing blueberry that germination rate is high.
Technical scheme: the present invention is achieved through the following technical solutions:
A kind of seed dormant method of blueberry of removing, is characterized in that, comprises the following steps:
(1) autumn by the end of October~early November, in the time that blueberry fruit is atropurpureus, gather in time; The fruit of gathering is put into water and is soaked and dry in the shade to hand-laundering out white seed after pulp deliquescing for 2~3 days, for subsequent use;
(2) add clean fine sand to rub after 10~20min in the seed of step (1), by seed and sand seed by volume: wet husky=mix at 1: 2, wet husky humidity is that hand is pinched not water outlet and is advisable, with husky Tibetan after 800~1000 times of medicine liquid sprays sterilizations of 50% carbendazol wettable powder dilution;
(3) at the beginning of by the end of February to 3 month, step (2) seed is sieved early spring in next year after by HORMONE TREATMENT, be placed in 25 ℃ of temperature, vernalization in the greenhouse of humidity 80%, radicle on the 5th~7 is broken through seed coat and is germinateed, and now can be seeded in seedling bed.
2, a kind of seed dormant method of blueberry of removing according to claim 1, it is characterized in that: the described sand Tibetan method of step (2) is, first mixed husky seed is placed in to 30 ℃ of temperature, in the greenhouse of humidity 80% after 5 days, then is placed in the environment 50~70 days of 0~5 ℃ of temperature.
3, a kind of seed dormant method of blueberry of removing according to claim 1, is characterized in that: the HORMONE TREATMENT of step (3), is that seed is immersed in to 4~8h in concentration 50~100mg/L heteroauxin solution, then rinses well with clear water.
Beneficial effect: the seed dormant method of releasing blueberry of the present invention, treated blueberry percentage of seedgermination reaches more than 95%, and germination required time is short, the planting seed neat and consistent of emerging behind seedbed, growth of seedling stalwartness; This method is easy to operation, has that cost is low, technical requirement is simple, the advantage such as easily applies, and on blueberry seedling breeding is produced, has important application and promotional value.
Embodiment
Embodiment 1
(1) autumn by the end of October~early November, in the time that blueberry fruit is atropurpureus, gather in time; The fruit of gathering is put into water and is soaked and dry in the shade to hand-laundering out white seed after pulp deliquescing for 2~3 days, for subsequent use;
(2) add clean fine sand to rub after 20min in the seed modulating, by seed and sand seed by volume: wet husky=mix at 1: 2, wet husky humidity is that hand is pinched not water outlet and is advisable, with husky Tibetan after 800 times of medicine liquid spray sterilizations of 50% carbendazol wettable powder dilution, first mixed husky seed is placed in to 30 ℃ of temperature, in the greenhouse of humidity 80% after 5 days, then be placed in the environment 50 days of 0~5 ℃ of temperature;
(3) after husky a surname's seed being sieved at the beginning of by the end of February to 3 month in early spring in next year, seed is put into concentration 50mg/L heteroauxin solution and soak 8h, rinse well with clear water again, be placed in 25 ℃ of temperature, vernalization in the greenhouse of humidity 80%, radicle on the 5th~7 is broken through seed coat and is germinateed, and now can be seeded in seedling bed, and germination rate reaches 95.3%.
Embodiment 2
(1) autumn by the end of October~early November, in the time that blueberry fruit is atropurpureus, gather in time; The fruit of gathering is put into water and is soaked and dry in the shade to hand-laundering out white seed after pulp deliquescing for 2~3 days, for subsequent use;
(2) add clean fine sand to rub after 10min in the seed modulating, by seed and sand seed by volume: wet husky=mix at 1: 2, wet husky humidity is that hand is pinched not water outlet and is advisable, with husky a surname after 1000 times of medicine liquid spray sterilizations of 50% carbendazol wettable powder dilution, first mixed husky seed is placed in to 30 ℃ of temperature, in the greenhouse of humidity 80% after 5 days, then be placed in the environment 70 days of 0~5 ℃ of temperature;
(3) after the seed of at the beginning of by the end of February to 3 month in early spring in next year, sand being hidden sieves, seed is put into concentration 100mg/L heteroauxin solution and soak 4h, rinse well with clear water again, be placed in 25 ℃ of temperature, vernalization in the greenhouse of humidity 80%, radicle on the 5th~7 is broken through seed coat and is germinateed, and now can be seeded in seedling bed, and germination rate reaches 98.4%.
Wherein take embodiment 2 as preferred embodiment.
Below disclose the present invention with preferred embodiment, so it is not intended to limiting the invention, and all employings are equal to replaces or the technical scheme that obtains of equivalent transformation mode, within all dropping on protection scope of the present invention.