CN101455140A

CN101455140A - Yam bulbil dormancy-breaking germination-accelerating method

Info

Publication number: CN101455140A
Application number: CNA2009100940228A
Authority: CN
Inventors: 郭华春; 龙雯虹; 王琼
Original assignee: Yunnan Agricultural University
Current assignee: Yunnan Agricultural University
Priority date: 2009-01-09
Filing date: 2009-01-09
Publication date: 2009-06-17
Anticipated expiration: 2029-01-09
Also published as: CN101455140B

Abstract

The present invention discloses a Chinese yam Dioscorea opposita dormancy braking and seed germination promoting method, comprising steps of soaking the Dioscorea opposita harvested in the current year in a 500 time carbendazim solution for 10 minutes, taking out the Dioscorea opposita, cleaning the Dioscorea opposita by water and drying the water on the surface of the Dioscorea opposita in air; making up a 1mg/L -100mg/L aqueous solution of 40% paclobutrazol wettable powder; putting disinfected Dioscorea opposita into a flat-bottom container to soak with a solution depth of about two thirds of the diameter of Dioscorea opposita, processing under a temperature of 25 DEG C, replacing paclobutrazol solution one time every week, washing the Dioscorea opposita by running water before replacing the solution, drying the water on the surface of the Dioscorea opposita in the air, and then putting the dried Dioscorea opposita in the flat-bottom container for soaking by the paclobutrazol to germination; Therefore, the germination percentage of the Dioscorea opposita of the harvest year can reach to 25%-50% by using the method, more than 90% Dioscorea opposita can be induced to be germinated and take root in the end of January of the second year, which greatly advances the germination period of Dioscorea opposita and solves the problems of long period of dormancy and difficult germination of Chinese yam Dioscorea opposita.

Description

The method of Yam bulbil dormancy-breaking germination-accelerating

Technical field:

The present invention relates to the method for agricultural planting biological technical field, particularly a kind of Yam bulbil dormancy-breaking germination-accelerating.

Technical background:

Chinese yam is the Dioscoreaceae yam, and its underground stem tuber that expands contains mineral elements such as abundant starch, vitamin, protamine and phosphorus, calcium, magnesium, iron, zinc, copper, potassium, and mouthfeel is good, is to suit the taste of both old and young, hundred eat the nutrient excellent product of not minding.China's tradition traditional Chinese medical science thinks that the flat flavor of Chinese yam property is sweet, go into lung, spleen, stomach, functions such as invigorating the spleen, kidney tonifying, beneficial essence are arranged, weakness of the spleen and the stomach to person in middle and old age, lose the appetite, cough that to breathe heavily phlegm many, and diabetes, constipation all have certain curative effect, are the main components of multiple Chinese patent drugs such as ginseng Siberian cocklebur bighead atractylodes rhizome ball, Yougui Wan, the kidney-Yang-Reinforcing Bolus, the Pill of Anemarrhena, Phellodendrom and Rehmannia, Liuwei Dihuang Wan.

Bulbil is a bulbil, and is born in the climing axil place of going up of Chinese yam stem, and is rounded or oval, is a species transformation stem, and after resting stage finished, can germinate formed new plant.The bulbil quantity that every strain Chinese yam is produced is many, and bulbil helps the rapid popularization of the good new varieties of Chinese yam as organ of multiplication reproduction coefficient height.

But bulbil resting stage is long, and gather in the crops general October to November, and after rudiment in March in next year to April, sowing can be sprouted, and resting stage reaches 4 months to 6 months.Bulbil is in the adapt circumstance condition in sleep procedure, it can only send adventive root, can not germinate and produce effective root.Bulbil resting stage length has influence in good time cultivation." the Changjiang river vegetables " o. 11th discloses " Chinese yam bulbil different disposal is to emerging and the influence of output ", and this research is to carry out in April in spring, and the bulbil with gibberellin treatment resting stage finishes promotes after planting early to emerge.Do not see open report as yet about breaking the method that the bulbil dormancy impels advance germination.

Summary of the invention:

The object of the present invention is to provide a kind of method of Yam bulbil dormancy-breaking germination-accelerating, to solve Chinese yam bulbil long, the difficult problem of germinateing of resting stage.Purpose of the present invention is achieved through the following technical solutions:

Handling with chemical reagent then that bulbil is gathered, make bulbil just reach 25%-50% at the germination rate then of results, in late January, second can be induced the bulbil germination more than 90% and be taken root, greatly done sth. in advance the germination period of bulbil, results provide strong technical guarantee for Chinese yam cultivation ahead of time reaches ahead of time.

Concrete steps are as follows:

1, bulbil sterilization: select unabroken bulbil, soaked bulbil 10 minutes, pull the back water out and rinse well, dry the moisture on bulbil surface with 500 times of carbendazim solutions.

2, the preparation of chemical reagent: the aqueous solution that 40% paclobutrazol wetting powder is made into 1mg/L-100mg/L.

3, chemical reagent is handled bulbil: the bulbil that will disinfect is in advance put into Flat bottom container, note not piling pressure between bulbil, inject paclobutrazol solution soaking bulbil in container, solution deep is about 2/3 of a bulbil diameter, places under 25 ℃ of temperature to handle.In the processing procedure, change paclobutrazol solution weekly once, change the preceding elder generation of solution and wash bulbil with flowing water, remove the oozy dope of bulbil, in case bulbil is gone rotten, cleaning finishes dries the bulbil surface moisture, puts into Flat bottom container again and continues to use the paclobutrazol solution soaking.

4, with the bulbil sowing in time of germinateing.

Advantage of the present invention or good effect are:

1), shortens resting stage, stratification.Traditional cultivation method is after annual 3, the bulbil rudiment in April, just to sow, after planting take more than 20 day in addition after 1 month bulbil just be unearthed formation seedling, so required time is long.Handle with method provided by the invention, effective root can be germinateed and produce to bulbil just then after gathering, can shift to an earlier date the planting season of Chinese yam.

2), be convenient to plant according to germination in good time, improve the plant strain growth regularity, be convenient to management.In the traditional cultivation method, the bulbil insemination and emergence is irregular, is not easy to cultivation management.Use the method, can plant in batches, improve the plant strain growth regularity, be convenient to management according to bulbil germination and the time of taking root.

Embodiment

Embodiment 1

1), on December 6th, 2006 selected results, unabroken bulbil then, soaked bulbils 10 minutes with 500 times of carbendazim solutions, pulled afterwards water out and rinsed well, dried the moisture on bulbil surface.

2), 40% paclobutrazol wetting powder is made into the aqueous solution of 1mg/L.

3), bulbil is put into the culture dish of diameter 10cm, inject the paclobutrazol solution of 1mg/L, solution deep is about 2/3 of a bulbil diameter, places incubator to handle under 25 ℃ of temperature.In the processing procedure, the paclobutrazol solution of changing 1mg/L weekly once, earlier wash bulbil before changing solution with flowing water, remove the oozy dope of bulbil, in case bulbil is gone rotten, cleaning finishes and dries the bulbil surface moisture, puts into the paclobutrazol solution soaking that Flat bottom container continues to use 1mg/L again.The processing of soaking bulbil with clear water is made as contrast.Each processing has 50 bulbils, establishes three repetitions.

4), observe the bulbil germination, the bulbil of germinateing is planted.On December 31st, 2006, the bulbil germination rate of the paclobutrazol solution-treated of 1mg/L reaches on January 21st, 30%, 2007, and 92% bulbil is germinateed, and the final germination rate of handling with clear water of contrast has only 8%.The bulbil kind of taking root, germinateing is put into 25 ℃ of incubators and is cultivated in plastic basin humous is housed, and 96% seedling survives normal growth after two weeks.

Embodiment 2

1), with the step 1 of embodiment 1.

2), 40% paclobutrazol wetting powder is made into the aqueous solution of 10mg/L.

3), except that employed paclobutrazol concentration is the 10mg/L, all the other steps are with the step 3) of embodiment 1.

4), observe the bulbil germination, the bulbil of germinateing is planted.On December 31st, 2006 reached on January 21st, 50%, 2007 with the bulbil germination rate of the paclobutrazol solution-treated of 10mg/L, and 96% bulbil is germinateed, and the final germination rate of handling with clear water of contrast has only 8%.The bulbil kind of germinateing is put into 25 ℃ of incubators and is cultivated in plastic basin humous is housed, and 97% seedling survives normal growth after two weeks.

Embodiment 3

1), with the step 1 of embodiment 1.

2), 40% paclobutrazol wetting powder is made into the concentration of 100mg/L.

3), except that employed paclobutrazol concentration is the 100mg/L, all the other steps are with the step 3) of embodiment 1.

4), observe the bulbil germination, the bulbil of germinateing is planted.On December 31st, 2006 reached on January 21st, 25%, 2007 with the bulbil germination rate of the paclobutrazol solution-treated of 100mg/L, and 90% bulbil is germinateed, and the contrast germination rate of handling with clear water has only 6%.The bulbil kind of taking root, germinateing is put into 25 ℃ of incubators and is cultivated in plastic basin humous is housed, and 95% seedling survives normal growth after two weeks.

Claims

1, a kind of method of Yam bulbil dormancy-breaking germination-accelerating is characterized in that step is as follows:

1), gather then in bulbil, soaked bulbils 10 minutes with 500 times of carbendazim solutions, pull the back water out and rinse well, the moisture that dries the bulbil surface is standby;

2), 40% paclobutrazol wetting powder is made into the aqueous solution of 1mg/L-100mg/L;

3), will put into Flat bottom container through the bulbil that step 1) disinfects, in container, inject the paclobutrazol solution soaking bulbil of 1mg/L, solution deep is 2/3 of a bulbil diameter, place under 25 ℃ of temperature and handle, change paclobutrazol solution weekly once, earlier wash bulbil with flowing water before the solution that renews, cleaning finishes dries the bulbil surface moisture, puts into Flat bottom container again and continues with the paclobutrazol solution soaking to germinateing.