CN103168589A - Dormancy breaking method for Chinese yam bulbels - Google Patents

Abstract

The invention discloses a dormancy breaking method for Chinese yam bulbels and belongs to the technical field of biology. In the year when the bulbels are harvested, the bulbels in the same size are selected to be aired under sunlight for about two days. The bulbel are fumigated for 3 days to 5 days by fumigation liquid made of ethylene chlorohydrin, dichloroethane and carbon tetrachloride in a volume ratio of 7:3:1. The middle portion of each bulbel is punctured by a needle in the depth of about 0.3cm and the bulbels are soaked in 1% of thiourea solution for 120 minutes, taken out and cleaned by water. The water on the surface of each bulbel is aired, the bulbels are placed in a culture dish with filter paper laid inside and cultured in darkness under the environment with the temperature at about 25 DEG C. Before culture, the filter paper is soaked with water and moisture on the filter paper is maintained through spraying water by a wash bottle every 3 days. The bulbels are cultured for 28 days according to the method, 85% of the bulbels can be led to germinate and take roots, germination period of the bulbels is brought forward and the problems that the Chinese yam bulbels are long in dormancy period and difficult to germinate are solved.

Description

A kind of Chinese yam bulbil is abolished dormancy method

Technical field

The present invention's " method of the broken dormancy of a kind of Chinese yam bulbil " is to be passed through the modes such as chemical reagent is stifling, immersion to make its sprouting by the Chinese yam bulbil, belongs to biological technical field.

Background technology

Chinese yam (Dioscorea Opposita Thunb.) is the perennial herbaceous stem prehensile of Dioscoreaceae Dioscorea liane, and all there is plantation various places, China north and south.Because it has higher nutritive value and medical value, be medicinal material and the traditional export commodity in China tunnel, the states such as its product situation of selling well Southeast Asia and Japan.Chinese yam adopts stem tuber to breed usually, and reproduction coefficient is low, and production cost is high, and utilizes for a long time the stem tuber breeding to make the continuous accumulation of virus cause kind of a sexual involution, has had a strong impact on the raising of Quality and yield.And the bulbil quantity of Chinese yam stem cissoid armpit place's growth is many, and bulbil is made propagating materials, can not only reduce the Chinese yam production cost, accelerates the popularization of breeding, and is beneficial to the purification and rejuvenation of kind, solves the problem of kind of sexual involution.But bulbil resting stage is long, has limited and has begun sowing in good time in producing.Therefore, carry out very necessary that the broken dormant technology of Chinese yam bulbil shows.

Summary of the invention

Technical problem

The objective of the invention is both to find a kind of bulbil method of beginning sowing in good time, can reduce again the method for potato seed reproductive-cost and maintenance kind good characteristic, the method has that reproductive-cost is low, reproduction speed is fast, band virus less, easily keeps the advantages such as variety stability, can make up bulbil resting stage long, existing potato piece reproductive-cost is high, plants the problems such as sexual involution.

Technical scheme

The method of the broken dormancy of a kind of Chinese yam bulbil comprises the following steps:

(1) processing after bulbil is gathered: gather then, select bulbil of the same size under daylight airing 1-3 days;

(2) bulbil is stifling; Use dichlroroethanol: dichloroethane: the carbon tetrachloride volume ratio is stifling 3-5 days of 7:3:1 fumigation liquid;

(3) immersion of bulbil: with acupuncture bulbil middle part degree of depth 0.3cm, use the thiourea solution of mass ratio 1% to soak 120 minutes, pull rear water out and rinse well, dry the moisture on bulbil surface;

(4) cultivation of bulbil: the bulbil after soaking is placed in the culture dish that is covered with filter paper, in temperature is secretly to cultivate under the environment of 25 ℃, before cultivating, filter paper is soaked, later on every 3d with water of wash bottle sprinkling to keep moistening.

Use dichlroroethanol: dichloroethane: the carbon tetrachloride volume ratio is the stifling bulbil of 7:3:1 fumigation liquid, and its consumption fumigation liquid ml: bulbil g is 1:1000.

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Beneficial effect

The present invention can solve bulbil long problem of resting stage;

The present invention is conducive to solve the kind sexual involution that the long-term nutrition breeding causes, and is conducive to the purification and rejuvenation of kind;

The present invention has that reproductive-cost is low, reproduction speed is fast, band virus less, easily keeps the advantages such as variety stability.

Description of drawings

Accompanying drawing 1 contrast

The dark 28d that cultivates of accompanying drawing 2

Embodiment

Further illustrate the present invention below by the method based on the broken dormancy of a kind of Chinese yam bulbil.

1 materials and methods

1.1 material: day raw white Chinese yam bulbil

1.2 test method

After the bulbil of results is dried then, selecting the in the same size bulbil to adopt the thiocarbamide of stifling and 1% to soak two kinds of Combination of Methods processes, stifling mixture formula is dichlroroethanol: dichloroethane: carbon tetrachloride (the Chengdu Long Huagongshijichang of section) is the 7:3:1(volume ratio), fumigation liquid ml: bulbil g is 1:1000, will be with about acupuncture bulbil middle part degree of depth 0.3cm before thiocarbamide soaks, the rear bulbil that is disposed is placed in the culture dish that is covered with filter paper, vernalization under the dark condition of 25 ℃.10 bulbils of each culture dish, twice repetition.

1.2.1 test is processed:

Process 1: the stifling 1d of stifling mixed liquor processes 2: the stifling 3d of stifling mixed liquor

Process 3: the stifling 5d of stifling mixed liquor processes 4: the stifling 7d of stifling mixed liquor

Process 5: stifling mixed liquor is stifling+thiocarbamide processes, first with after the stifling 1d of stifling mixed liquor, then will fumigate process after bulbil be placed in 1% thiourea solution and soaked 120 minutes;

Process 6: stifling mixed liquor is stifling+thiocarbamide processes, first with after the stifling 3d of stifling mixed liquor, then will fumigate process after bulbil be placed in 1% thiourea solution and soaked 120 minutes;

Process 7: stifling mixed liquor is stifling+thiocarbamide processes, first with after the stifling 5d of stifling mixed liquor, then will fumigate process after bulbil be placed in 1% thiourea solution and soaked 120 minutes;

Process 8: stifling mixed liquor is stifling+thiocarbamide processes, first with after the stifling 7d of stifling mixed liquor, then will fumigate process after bulbil be placed in 1% thiourea solution and soaked 120 minutes;

Process 9:CK.

1.2.3 pilot survey:

After being disposed, every germination rate of 7d investigation, calculate two mean values that repeat germination rate.

2 results and analysis

As can be seen from Table 1, processing 6 effects are best, begin to germinate after cultivating 14d, and after 28d, germination rate reaches 85%, process 7 cultivation 28d germination rates and reach 75%, and processing 8 begins to germinate after cultivating 21d, reaches 30% after 28d.Therefore it is best that method stifling and that thiocarbamide soaks is adopted in the broken dormancy of bulbil, but the fumigation liquid fumigation time is unsuitable long, and stifling 3-5d is comparatively suitable.

The germination rate of table 1 different disposal bulbil

Time	Process 1	Process 2	Process 3	Process 4	Process 5	Process 6	Process 7	Process 8	Process 9
										7d	0	0	0	0	0	0	0	0	0
14d	0	0	0	0	0	10%	10%	0	0
										21d	0	0	0	0	0	10%	10%	10%	0
28d	0	0	0	0	0	85%	75%	30%	0
										35d	0	0	0	0	0	85%	75%	30%	0

Claims (2)

1. a Chinese yam bulbil is abolished dormancy method, comprises the following steps:

(1) processing after bulbil is gathered: gather then, select bulbil of the same size under daylight airing 1-3 days;

(2) bulbil is stifling; Use dichlroroethanol: dichloroethane: the carbon tetrachloride volume ratio is stifling 3-5 days of 7:3:1 fumigation liquid;

(3) immersion of bulbil: with acupuncture bulbil middle part degree of depth 0.3cm, use the thiourea solution of mass ratio 1% to soak 120 minutes, pull rear water out and rinse well, dry the moisture on bulbil surface;

(4) cultivation of bulbil: the bulbil after soaking is placed in the culture dish that is covered with filter paper, in temperature is secretly to cultivate under the environment of 25 ℃, before cultivating, filter paper is soaked, later on every 3d with water of wash bottle sprinkling to keep moistening.

2. a kind of Chinese yam bulbil is abolished dormancy method according to claim 1, it is characterized in that: use dichlroroethanol: dichloroethane: the carbon tetrachloride volume ratio is the stifling bulbil of 7:3:1 fumigation liquid, and consumption fumigation liquid ml: bulbil g is 1:1000.

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