CN105940893B - The fast breeding method of fruit of Rangoon creeper's seedling - Google Patents
The fast breeding method of fruit of Rangoon creeper's seedling Download PDFInfo
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- CN105940893B CN105940893B CN201610259481.7A CN201610259481A CN105940893B CN 105940893 B CN105940893 B CN 105940893B CN 201610259481 A CN201610259481 A CN 201610259481A CN 105940893 B CN105940893 B CN 105940893B
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- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 48
- 238000009395 breeding Methods 0.000 title claims abstract description 30
- 244000044425 Quisqualis indica Species 0.000 title claims abstract 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 72
- 239000003337 fertilizer Substances 0.000 claims abstract description 33
- 239000010455 vermiculite Substances 0.000 claims abstract description 24
- 235000019354 vermiculite Nutrition 0.000 claims abstract description 24
- 229910052902 vermiculite Inorganic materials 0.000 claims abstract description 24
- 239000010451 perlite Substances 0.000 claims abstract description 23
- 235000019362 perlite Nutrition 0.000 claims abstract description 23
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000005286 illumination Methods 0.000 claims abstract description 11
- 239000004576 sand Substances 0.000 claims abstract description 11
- 230000004345 fruit ripening Effects 0.000 claims abstract description 10
- 238000009331 sowing Methods 0.000 claims abstract description 10
- 241000894007 species Species 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 7
- 241000209094 Oryza Species 0.000 claims description 25
- 235000007164 Oryza sativa Nutrition 0.000 claims description 25
- 235000009566 rice Nutrition 0.000 claims description 25
- 238000005406 washing Methods 0.000 claims description 25
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 24
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims description 24
- 241000894006 Bacteria Species 0.000 claims description 16
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 claims description 14
- 229940033663 thimerosal Drugs 0.000 claims description 14
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 12
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 12
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 12
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 12
- 235000011151 potassium sulphates Nutrition 0.000 claims description 12
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 claims description 11
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 claims description 11
- 235000019837 monoammonium phosphate Nutrition 0.000 claims description 11
- 238000002791 soaking Methods 0.000 claims description 11
- 230000000813 microbial effect Effects 0.000 claims description 10
- 239000007921 spray Substances 0.000 claims description 8
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 7
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 7
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 7
- 241000233866 Fungi Species 0.000 claims description 6
- 230000010355 oscillation Effects 0.000 claims description 6
- 239000012286 potassium permanganate Substances 0.000 claims description 6
- 241000194108 Bacillus licheniformis Species 0.000 claims description 5
- 244000063299 Bacillus subtilis Species 0.000 claims description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 5
- 239000005416 organic matter Substances 0.000 claims description 5
- 230000000243 photosynthetic effect Effects 0.000 claims description 5
- 239000011159 matrix material Substances 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 4
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 3
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 239000002686 phosphate fertilizer Substances 0.000 claims description 2
- 239000002435 venom Substances 0.000 claims description 2
- 210000001048 venom Anatomy 0.000 claims description 2
- 231100000611 venom Toxicity 0.000 claims description 2
- 230000001488 breeding effect Effects 0.000 abstract description 11
- 238000000034 method Methods 0.000 abstract description 6
- 230000007226 seed germination Effects 0.000 abstract description 6
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 4
- 244000108662 Combretum indicum Species 0.000 description 32
- 230000035784 germination Effects 0.000 description 6
- 238000010899 nucleation Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002386 leaching Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000545744 Hirudinea Species 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000011435 rock Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000221032 Combretaceae Species 0.000 description 1
- 241000721047 Danaus plexippus Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 201000009361 ascariasis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 201000001883 cholelithiasis Diseases 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 230000024241 parasitism Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 230000035040 seed growth Effects 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The invention discloses a kind of fast breeding methods of fruit of Rangoon creeper's seedling, and step is: acquisition current year receives novel species after autumn end fruit maturation, removes shell, takes out seed；1:3 is mixed by volume with wet basis matter after the surface of the seed disinfection, is placed in constant temperature vernalization 2-3 days in 30-35 DEG C of illumination box；The wet basis matter obtained after vernalization and seed are directly broadcasted sowing on Light media seedbed, not doing any covering, nursery temperature is 30-35 DEG C, keep seedbed surface wettability, it is primary that water soluble phosphorous fertilizer is sprayed after culture two weeks, nursery 30-45 days, can be out of the garden when height of seedling reaches 10-30cm；Wherein, wet basis matter is one of sand, perlite, vermiculite；Light media is the mixture of one or both of thick vermiculite, thick perlite with turf.The method can significantly improve percentage of seedgermination, planting percent, the fast breeding of fruit of Rangoon creeper's seedling is realized, while also having very high practical application value to the work of fruit of Rangoon creeper's breeding of new variety.
Description
Technical field
The present invention relates to nursery stock production raising technology field, specifically a kind of fast breeding side of fruit of Rangoon creeper's seedling
Method.
Background technique
Fruit of Rangoon creeper's crude drug source belongs to the dry mature fruit of the fruit of Rangoon creeper in the combretaceae fruit of Rangoon creeper, the function with killing insect and eliminating accumulation
Effect, is usually used in paediatrics parasitism ascariasis.Fruit of Rangoon creeper original plant is to climb up by holding on to shape shrub, with luxuriant foliage and spreading branches in leafy profusion, pattern is bright-coloured, is south China
The common sight leaf decorative flower in area.The fruit of Rangoon creeper is a kind of with higher medicinal and ornamental value plant, and cultivation prospect is non-
It is often wide.
Fruit of Rangoon creeper's seminal propagation can both improve the breed, and be used for breeding of new variety, and seedling quality and breeding can be improved
Speed is produced for scale breeding.But since fruit of Rangoon creeper's seed is wrapped up by hard shell, the permeable breathable difference of shell and
To the mechanical restriction effect that embryonic development has, difficulty water suction, difficult broken shell growth, germination percentage are low when seed being caused to be sprouted, and kind shell is system
The about serious problems of seminal propagation.The artificial cultivation of the upper fruit of Rangoon creeper is produced at present based on cutting propagation, and cuttage seeding is able to maintain mother
This character can be such that the time blossomed and beared fruit does sth. in advance, but the root system of cuttage seeding is weaker, hat phenomenon partially easily occur, and the service life is shorter than real
Raw seedling, seedling quality are poor.And conventional seminal propagation bud ratio is extremely low, seedling is very little, is unable to reach the requirements of breeding.Therefore,
The nursery field of medicinal plant farming and afforestation industry be large-scale cultivation fruit of Rangoon creeper strong sprout, scientific research field be select it is more
Plant type, leaf, pattern, florescence different fruit of Rangoon creeper's new varieties, need that a kind of low cost, reproduction speed is fast, planting percent is high, nursery stock
Seedling mating system quality, easy to operate.
Summary of the invention
It is excellent it is an object of the invention to solve at least the above problems and/or defect, and provide at least to will be described later
Point.
It is also an object of the present invention to provide a kind of fast breeding methods of fruit of Rangoon creeper's seedling.It is made by abolishing
Gentleman's seed hull takes kind, vernalization processing and nursery culture, significantly improves percentage of seedgermination, planting percent, realizes that the fruit of Rangoon creeper is grown directly from seeds
The fast breeding of seedling, while being also of very high actual application value in scientific research to the work of fruit of Rangoon creeper's breeding of new variety.
In order to realize these purposes and other advantages according to the present invention, the fast breeding side of fruit of Rangoon creeper's seedling is provided
Method:
S1, seed selection take kind: acquisition current year receives novel species after autumn end fruit maturation, removes shell, takes out seed；
S2, vernalization processing: 1:3 is mixed by volume with wet basis matter after the surface of the seed is sterilized, and is placed in 30-35 DEG C of illumination
Constant temperature vernalization 2-3 days in incubator；
S3, nursery culture: wet basis matter and seed that vernalization is handled directly are broadcasted sowing on Light media seedbed, do not appointed
What is covered, and nursery temperature is 30-35 DEG C, is periodically sprinkled water primary daily, is kept seedbed surface wettability, spray water after cultivating two weeks
Dissolubility phosphate fertilizer is primary, after nursery 30-45 days, can be out of the garden when height of seedling reaches 10-30cm；
Wherein, wet basis matter is one of sand of the relative water content not less than 80%, perlite, vermiculite；
Light media is the mixture with turf of thick vermiculite, one or both of thick perlite that granularity is 3-5mm.
Preferably, the specific steps that the surface of the seed sterilizes in S2 are as follows: select mass fraction 0.05%-0.15% permanganic acid
Potassium or mass fraction 0.5%-2% sodium hypochlorite are thimerosal, and by thimerosal and seed 3:1 mixing by volume, soak seed 10-30
Minute, temperature control at 20-30 DEG C, after clear water rinse seed 3-5 times.
Preferably, it is needed before the surface of the seed disinfection by rice washing water seed soaking in S2: first by rice washing water in 40KHz frequency
Lower sonic oscillation 3-5 minutes, then 2:1 is mixed by volume with seed, and 4-5h is impregnated under the conditions of 30-40 DEG C of temperature, takes out kind
Son, rice washing water are continued to employ.
Preferably, wet basis matter passes through pretreatment in S2: calcium nitrate, potassium sulfate, phosphoric acid being added in the rice washing water continued to employ
Ammonium dihydrogen, magnesium sulfate formation calcium nitrate mass concentration are 354mg/L, potassium sulfate mass concentration is 607mg/L, ammonium dihydrogen phosphate matter
The solution that amount concentration is 96mg/L, magnesium sulfate mass concentration is 185mg/L impregnates wet basis matter for 24 hours, soak as soak
With wet matrix volume ratio 3:1.
Preferably, Light media is the thick vermiculite of volume ratio: turf=3:7, thick perlite: turf=3:7, thick leech in S3
Stone: thick perlite: any one in turf=1:1:1.
Preferably, it also added microbial-bacterial fertilizer in Light media, Light media and microbial-bacterial fertilizer volume ratio are 10:1, micro-
Microbial bacteria and quality of organic matter ratio are 1:84 in bio-bacterial manure, and microbial bacteria includes the photosynthetic bacteria of mass ratio 2:1:1:2, mycorhiza
Fungi, bacillus licheniformis and bacillus subtilis.
Preferably, the long 80cm in seedbed in S3, wide 35cm, deep 10cm are sown into 200, seed.
Preferably, it is that relative water content reaches 80% or more that seedbed surface wettability is kept in S3.
Preferably, in S3 water soluble phosphorous fertilizer be mass fraction 1% calcium superphosphate leachate.
The present invention is include at least the following beneficial effects:
The first, based on cutting propagation, the present invention acquires fruit of Rangoon creeper's artificial cultivation after selecting autumn end fruit of Rangoon creeper fruit maturation
Current year receive novel species breeding, since seed is wrapped up by hard shell, the permeable breathable of shell is poor, directly sows germination percentage pole
It is low, therefore shell is removed, it takes out seed and releases the obstruction that shell sprouts seed, significantly improve percentage of seedgermination, then by urging
Bud and nursery, which finally obtain, grows vigorous, well developed root system, the seedling lasted a long time；
The second, the present invention selects damp sand, wet perlite, any in wet vermiculite according to the germination characteristic of fruit of Rangoon creeper's seed
A kind of to be used as matrix, 1:3 is mixed by volume with wet basis matter after the surface of the seed is sterilized, and is placed in 30-35 DEG C of illumination box
It interior constant temperature vernalization 2-3 days, is sowed again after so that seed is showed money or valuables one carries unintentionally, significantly improves germination speed and germination regularity；
Third, the seedling characteristic of the invention according to fruit of Rangoon creeper's seed, the wet basis matter and seed that vernalization is handled are direct
It broadcasts sowing on Light media seedbed, not doing any covering, selected matrix is all Light media, and moisture and nutrition can seep mutually
Thoroughly, it is quickly unearthed so not covering and being more advantageous to seed；
4th, the present invention select rice washing water seed soaking, by rice washing water sonic oscillation 3-5 minutes under 40KHz frequency, then with
2:1 is mixed seed by volume, and 4-5h is impregnated under the conditions of 30-40 DEG C of temperature, and rice washing water is rich in starch, and starch can more after ultrasound
It is evenly soluble in water, the kind skin of fruit of Rangoon creeper's seed can be softened for soaking seed, reduce the resistance of germination, effect is better than conventional seed soaking side
Method；
5th, the present invention forms leaching for calcium nitrate, potassium sulfate, ammonium dihydrogen phosphate, magnesium sulfate are added in the rice washing water continued to employ
Liquid is steeped, impregnates wet basis matter for 24 hours, it is attached in wet basis matter that rice washing water can increase calcium nitrate, potassium sulfate, ammonium dihydrogen phosphate, magnesium sulfate
Put forth effort, while its own can also be used as fertilizer rich in various microelements, these fertilizer are stored in stromal surface and gap, are
Seed growth provides the nutrition for being easy to absorb, and has the function of mutually cooperateing with, dramatically speeds up the speed of growth of seedling；
6th, the present invention also added microbial-bacterial fertilizer in Light media, and microbial-bacterial fertilizer not only provides organic fertilizer, and
And the microbial bacteria contained by it can also inhibit fungi and seedling root disease and pest in soil, enhance seedling disease resistance, improve
The supply situation of nutrient, aeration status and porousness in soil promote seedling root growth.
Further advantage, target and feature of the invention will be partially reflected by the following instructions, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
Elaborate below with reference to embodiment to the present invention, with enable those of ordinary skill in the art refering to this specification after
It can implement accordingly.
<embodiment 1>
The present invention provides the fast breeding methods of fruit of Rangoon creeper's seedling, comprising the following steps:
Step 1: seed selection takes kind: acquisition current year receives novel species after autumn end fruit maturation, removes shell, takes out seed；
Step 2: vernalization is handled: selection 0.05% potassium permanganate of mass fraction is thimerosal, and thimerosal and seed are pressed body
Product than 3:1 mix, soak seed 30 minutes, 20 DEG C of temperature, after clear water rinse seed 3 times, after the surface of the seed is sterilized with damp sand
1:3 is mixed by volume, and damp sand relative water content is not less than 80%, is placed in constant temperature vernalization 2 days in 30 DEG C of illumination box；
Step 3: nursery culture: damp sand and seed that vernalization is handled directly being broadcasted sowing in the thick vermiculite of volume ratio: turf
On the Light media seedbed of=3:7, any covering is not done, and thick vermiculite granularity is 3mm, the long 80cm in seedbed, wide 35cm, and deep 10cm is broadcast
Enter 200, seed.Nursery temperature is 30 DEG C, and timing daily is sprinkled water once, and seedbed surface wettability is kept, and relative water content reaches
80% or more, spray that water soluble phosphorous fertilizer is primary after cultivating two weeks, water soluble phosphorous fertilizer is that the calcium superphosphate of mass fraction 1% leaches
Liquid after nursery 30 days, can be out of the garden when height of seedling reaches 10cm.
<embodiment 2>
The present invention provides the fast breeding methods of fruit of Rangoon creeper's seedling, comprising the following steps:
Step 1: seed selection takes kind: acquisition current year receives novel species after autumn end fruit maturation, removes shell, takes out seed；
Step 2: vernalization is handled: selection 0.15% potassium permanganate of mass fraction is thimerosal, and thimerosal and seed are pressed body
Product than 3:1 mix, soak seed 10 minutes, 30 DEG C of temperature, after clear water rinse seed 5 times, after the surface of the seed is sterilized with wet treasure
1:3 is mixed Zhu Yan by volume, and wet perlite relative water content is not less than 80%, is placed in constant temperature in 35 DEG C of illumination box and urges
Bud 3 days；
Step 3: nursery culture: wet perlite and seed that vernalization is handled directly are broadcasted sowing in the thick pearl of volume ratio
Rock: on turf=3:7 Light media seedbed, not doing any covering, and thick perlite granularity is 5mm, the long 80cm in seedbed, wide 35cm,
Deep 10cm is sown into 200, seed, and nursery temperature is 35 DEG C, and timing daily is sprinkled water once, keeps seedbed surface wettability, opposite to contain
Water reaches 80% or more, cultivate and spray that water soluble phosphorous fertilizer is primary after two weeks, and water soluble phosphorous fertilizer is that mass fraction 1% crosses phosphorus
Sour calcium leachate after nursery 45 days, can be out of the garden when height of seedling reaches 25cm.
<embodiment 3>
The present invention provides the fast breeding methods of fruit of Rangoon creeper's seedling, comprising the following steps:
Step 1: seed selection takes kind: acquisition current year receives novel species after autumn end fruit maturation, removes shell, takes out seed；
Step 2: vernalization is handled: selection 0.10% potassium permanganate of mass fraction is thimerosal, and thimerosal and seed are pressed body
Product than 3:1 mix, soak seed 20 minutes, 25 DEG C of temperature, after clear water rinse seed 4 times, after the surface of the seed is sterilized with wet leech
1:3 is mixed stone by volume, and wet vermiculite relative water content is not less than 80%, is placed in constant temperature vernalization in 33 DEG C of illumination box
2.5 it；
Step 3: nursery culture: wet vermiculite and seed that vernalization is handled directly being broadcasted sowing in the thick vermiculite of volume ratio: thick
Perlite: on turf=1:1:1 Light media seedbed, any covering is not done, thick vermiculite, thick perlite granularity are 4mm, seedbed
Long 80cm, wide 35cm, deep 10cm are sown into 200, seed, and nursery temperature is 33 DEG C, and timing daily is sprinkled water once, keep seedbed table
Face is wet, and relative water content reaches 80% or more, cultivates and sprays that water soluble phosphorous fertilizer is primary, and water soluble phosphorous fertilizer is quality after two weeks
The calcium superphosphate leachate of score 1% after nursery 40 days, can be out of the garden when height of seedling reaches 20cm.
<embodiment 4>
The present invention provides the fast breeding methods of fruit of Rangoon creeper's seedling, comprising the following steps:
Step 1: seed selection takes kind: acquisition current year receives novel species after autumn end fruit maturation, removes shell, takes out seed；
Step 2: vernalization handle: rice washing water seed soaking, by rice washing water under 40KHz frequency sonic oscillation 3 minutes, so
2:1 is mixed by volume with seed afterwards, and 5h is impregnated under the conditions of 30 DEG C of temperature, takes out seed, rice washing water is continued to employ, in the rice washing continued to employ
It is 354mg/L, potassium sulfate quality that calcium nitrate, potassium sulfate, ammonium dihydrogen phosphate, magnesium sulfate are added in water and forms calcium nitrate mass concentration
The solution that concentration is 607mg/L, ammonium dihydrogen phosphate mass concentration is 96mg/L, magnesium sulfate mass concentration is 185mg/L is as leaching
Liquid is steeped, damp sand is impregnated for 24 hours, soak and damp sand volume ratio 3:1, selection 0.05% potassium permanganate of mass fraction is thimerosal,
By thimerosal and seed by volume 3:1 mix, soak seed 30 minutes, 20 DEG C of temperature, after clear water rinse seed 3 times, will plant
1:3 is mixed by volume with damp sand after sub- surface sterilization, is placed in constant temperature vernalization 2 days in 30 DEG C of illumination box；
Step 3: nursery culture: damp sand and seed that vernalization is handled directly being broadcasted sowing in the thick vermiculite of volume ratio: turf
On the Light media seedbed of=3:7, any covering is not done, thick vermiculite granularity is 3mm, it also added microbial-bacterial fertilizer in Light media,
Light media and microbial-bacterial fertilizer volume ratio are 10:1, and microbial bacteria and quality of organic matter ratio are 1:84, micro- life in microbial-bacterial fertilizer
Object bacterium bag includes photosynthetic bacteria, mycorrhizal fungi, bacillus licheniformis and the bacillus subtilis of mass ratio 2:1:1:2, the long 80cm in seedbed,
Wide 35cm, deep 10cm are sown into 200, seed, and nursery temperature is 30 DEG C, and timing daily is sprinkled water once, keep seedbed surface wettability,
Relative water content reaches 80% or more, cultivates and sprays that water soluble phosphorous fertilizer is primary, and water soluble phosphorous fertilizer is mass fraction 1% after two weeks
Calcium superphosphate leachate, after nursery 30 days, when height of seedling reach 10cm when can be out of the garden.
<embodiment 5>
The present invention provides the fast breeding methods of fruit of Rangoon creeper's seedling, comprising the following steps:
Step 1: seed selection takes kind: acquisition current year receives novel species after autumn end fruit maturation, removes shell, takes out seed；
Step 2: vernalization handle: rice washing water seed soaking, by rice washing water under 40KHz frequency sonic oscillation 4 minutes, so
2:1 is mixed by volume with seed afterwards, and 4h is impregnated under the conditions of 40 DEG C of temperature, takes out seed, rice washing water is continued to employ, in the rice washing continued to employ
It is 354mg/L, potassium sulfate quality that calcium nitrate, potassium sulfate, ammonium dihydrogen phosphate, magnesium sulfate are added in water and forms calcium nitrate mass concentration
The solution that concentration is 607mg/L, ammonium dihydrogen phosphate mass concentration is 96mg/L, magnesium sulfate mass concentration is 185mg/L is as leaching
Liquid is steeped, wet perlite is impregnated for 24 hours, soak and wet perlite volume ratio 3:1, selection 0.15% potassium permanganate of mass fraction is
Thimerosal, by thimerosal and seed, 3:1 is mixed by volume, is soaked seed 10 minutes, 30 DEG C of temperature, after clear water rinse seed 5
Secondary, 1:3 is mixed by volume with wet perlite after the surface of the seed is sterilized, and is placed in constant temperature vernalization 3 in 35 DEG C of illumination box
It；
Step 3: nursery culture: wet perlite and seed that vernalization is handled directly are broadcasted sowing in the thick pearl of volume ratio
Rock: on turf=3:7 Light media seedbed, not doing any covering, and thick perlite granularity is 5mm, be also added in Light media micro-
Bio-bacterial manure, Light media and microbial-bacterial fertilizer volume ratio are 10:1, and microbial bacteria is with quality of organic matter ratio in microbial-bacterial fertilizer
1:84, microbial bacteria include photosynthetic bacteria, mycorrhizal fungi, bacillus licheniformis and the bacillus subtilis of mass ratio 2:1:1:2, seedling
Bed long 80cm, wide 35cm, deep 10cm are sown into 200, seed, and nursery temperature is 35 DEG C, and timing watering daily is primary, keep seedbed
Surface wettability, relative water content reach 80% or more, cultivate and spray that water soluble phosphorous fertilizer is primary, and water soluble phosphorous fertilizer is matter after two weeks
The calcium superphosphate leachate for measuring score 1% after nursery 45 days, can be out of the garden when height of seedling reaches 25cm.
<embodiment 6>
The present invention provides the fast breeding methods of fruit of Rangoon creeper's seedling, comprising the following steps:
Step 1: seed selection takes kind: acquisition current year receives novel species after autumn end fruit maturation, removes shell, takes out seed；
Step 2: vernalization handle: rice washing water seed soaking, by rice washing water under 40KHz frequency sonic oscillation 5 minutes, so
2:1 is mixed by volume with seed afterwards, and 4.5h is impregnated under the conditions of 35 DEG C of temperature, takes out seed, and rice washing water is continued to employ, and is washed in a pan what is continued to employ
It is 354mg/L, potassium sulfate matter that meter Shui Zhong, which is added calcium nitrate, potassium sulfate, ammonium dihydrogen phosphate, magnesium sulfate and forms calcium nitrate mass concentration,
Measure concentration be 607mg/L, the solution conduct that ammonium dihydrogen phosphate mass concentration is 96mg/L, magnesium sulfate mass concentration is 185mg/L
Soak impregnates wet vermiculite for 24 hours, soak and wet vermiculite volume ratio 3:1, and selecting 0.10% sodium hypochlorite of mass fraction is to disappear
Venom, by thimerosal and seed, 3:1 is mixed by volume, is soaked seed 20 minutes, 25 DEG C of temperature, after clear water rinse seed 4 times,
1:3 is mixed by volume with wet vermiculite after the surface of the seed is sterilized, and is placed in constant temperature vernalization 2.5 days in 33 DEG C of illumination box；
Step 3: nursery culture: wet vermiculite and seed that vernalization is handled directly being broadcasted sowing in the thick vermiculite of volume ratio: thick
Perlite: on turf=1:1:1 Light media seedbed, any covering is not done, thick vermiculite, thick perlite granularity are 4mm, light base
Also added microbial-bacterial fertilizer in matter, Light media and microbial-bacterial fertilizer volume ratio are 10:1, in microbial-bacterial fertilizer microbial bacteria with
Quality of organic matter ratio is 1:84, microbial bacteria include the photosynthetic bacteria of mass ratio 2:1:1:2, mycorrhizal fungi, bacillus licheniformis and
Bacillus subtilis, the long 80cm in seedbed, wide 35cm, deep 10cm are sown into 200, seed, and nursery temperature is 35 DEG C, is periodically spilt daily
Water is primary, keeps seedbed surface wettability, and relative water content reaches 80% or more, sprays water soluble phosphorous fertilizer one after cultivating two weeks
Secondary, water soluble phosphorous fertilizer is the calcium superphosphate leachate of mass fraction 1%, after nursery 40 days, can be out of the garden when height of seedling reaches 20cm.
<comparative example 1>
By the fruit of Rangoon creeper's seed routine seed-soaking method clear water wrapped up by shell seed soaking 4-5h, after conventional method vernalization 2-3 days,
Directly it is seeded in nursery culture 45 days on generic media seedbed.
Influence of each embodiment of table 1 to fruit of Rangoon creeper's seed germination rate and planting percent
| Comparative example l | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 | |
| Bud ratio (%) | 3.1 | 79.3 | 79.8 | 80.4 | 87.3 | 87.1 | 87.7 |
| Planting percent (%) | 2.4 | 77.6 | 77.9 | 78.2 | 85.9 | 85.7 | 86.3 |
As can be seen from Table 1, fruit of Rangoon creeper's fruit takes kind by broken shell, with wet basis matter in illumination box constant temperature after seed disinfection
Vernalization carries out nursery culture on Light media seedbed, can significantly improve seed germination rate and planting percent；Also, seed is used
Rice washing water seed soaking, soak impregnate wet basis matter, and after adding microbial-bacterial fertilizer in Light media seedbed, can further increase makes monarch
Sub- percentage of seedgermination and planting percent.
<comparative example 2>
By fruit of Rangoon creeper's seedling of cutting survival, fruit of Rangoon creeper's seedling from seed compares in embodiment 1-6, seedling planting percent
For 77%-86%, cuttage seeding planting percent is 75%-88%, and seedling planting percent can compare favourably with cuttage seeding；Seedling root/shoot ratio
For 1:(3-5) well developed root system, and cuttage seeding root/shoot ratio is 1:(8-12) root system is less weaker；Also, seedling growing way is vigorous, raw
Long speed is significantly faster than that cuttage seeding.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and embodiment shown and described herein.
Claims (9)
1. a kind of fast breeding method of fruit of Rangoon creeper's seedling, characterized in that it comprises the following steps:
S1, seed selection take kind: acquisition current year receives novel species after autumn end fruit maturation, removes shell, takes out seed；
S2, vernalization processing: 1:3 is mixed by volume with wet basis matter after the surface of the seed is sterilized, and is placed in 30-35 DEG C of illumination cultivation
Constant temperature vernalization 2-3 days in case；
S3, nursery culture: wet basis matter and seed that vernalization is handled directly are broadcasted sowing on Light media seedbed, do not do any cover
Lid, nursery temperature are 30-35 DEG C, and timing watering daily is primary, keeps seedbed surface wettability, spray water solubility after cultivating two weeks
Phosphate fertilizer is primary, after nursery 30-45 days, can be out of the garden when height of seedling reaches 10-30cm；
Wherein, wet basis matter is one of sand of the relative water content not less than 80%, perlite, vermiculite；
Light media is the mixture with turf of thick vermiculite, one or both of thick perlite that granularity is 3-5mm.
2. the fast breeding method of fruit of Rangoon creeper's seedling as described in claim 1, which is characterized in that the surface of the seed sterilizes in S2
Specific steps are as follows: selecting mass fraction 0.05%-0.15% potassium permanganate or mass fraction 0.5%-2% sodium hypochlorite is to disappear
Venom, by thimerosal and seed, 3:1 is mixed by volume, is soaked seed 10-30 minute, temperature control at 20-30 DEG C, after clear water
It rinses seed 3-5 times.
3. the fast breeding method of fruit of Rangoon creeper's seedling as described in claim 1, which is characterized in that the surface of the seed sterilizes in S2
Before need to pass through rice washing water seed soaking, first then rice washing water is pressed into body with seed sonic oscillation 3-5 minutes under 40KHz frequency
Product is mixed than 2:1, and 4-5h is impregnated under the conditions of 30-40 DEG C of temperature, takes out seed, and rice washing water is continued to employ.
4. the fast breeding method of fruit of Rangoon creeper's seedling as claimed in claim 3, which is characterized in that wet basis matter is by pre- in S2
Processing: calcium nitrate, potassium sulfate, ammonium dihydrogen phosphate, magnesium sulfate formation calcium nitrate mass concentration are added in the rice washing water continued to employ is
354mg/L, potassium sulfate mass concentration are 607mg/L, ammonium dihydrogen phosphate mass concentration is 96mg/L, magnesium sulfate mass concentration is
The solution of 185mg/L impregnates wet basis matter for 24 hours, soak and wet matrix volume ratio 3:1 as soak.
5. the fast breeding method of fruit of Rangoon creeper's seedling as described in claim 1, which is characterized in that Light media is volume in S3
Than thick vermiculite: turf=3:7, thick perlite: turf=3:7, thick vermiculite: thick perlite: any one in turf=1:1:1
Kind.
6. the fast breeding method of fruit of Rangoon creeper's seedling as claimed in claim 5, which is characterized in that also added in Light media
Microbial-bacterial fertilizer, Light media and microbial-bacterial fertilizer volume ratio are 10:1, microbial bacteria and quality of organic matter ratio in microbial-bacterial fertilizer
For 1:84, microbial bacteria includes photosynthetic bacteria, mycorrhizal fungi, bacillus licheniformis and the bacillus subtilis of mass ratio 2:1:1:2.
7. the fast breeding method of fruit of Rangoon creeper's seedling as described in claim 1, which is characterized in that the long 80cm in seedbed, it is wide
35cm, deep 10cm are sown into 200, seed.
8. the fast breeding method of fruit of Rangoon creeper's seedling as described in claim 1, which is characterized in that keep seedbed surface in S3
It moistens and reaches 80% or more for relative water content.
9. the fast breeding method of fruit of Rangoon creeper's seedling as described in claim 1, which is characterized in that water soluble phosphorous fertilizer is in S3
The calcium superphosphate leachate of mass fraction 1%.
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| CN113016474B (en) * | 2021-03-03 | 2023-09-08 | 广西壮族自治区药用植物园 | Seedling raising method for radix zanthoxyli seedlings |
| CN113207580A (en) * | 2021-06-11 | 2021-08-06 | 成都市天地载生态农业有限公司 | Rapid seedling raising method for holboellia latifolia seeds |
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