CN104472062B - A kind of method promoting core fragrant plant seed germination - Google Patents
A kind of method promoting core fragrant plant seed germination Download PDFInfo
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- CN104472062B CN104472062B CN201410844940.9A CN201410844940A CN104472062B CN 104472062 B CN104472062 B CN 104472062B CN 201410844940 A CN201410844940 A CN 201410844940A CN 104472062 B CN104472062 B CN 104472062B
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- germination
- fragrant plant
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- plant seed
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- 238000000034 method Methods 0.000 title claims abstract description 26
- 230000007226 seed germination Effects 0.000 title claims abstract description 20
- 230000001737 promoting effect Effects 0.000 title claims abstract description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 9
- 238000011081 inoculation Methods 0.000 claims abstract description 5
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 239000003153 chemical reaction reagent Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000000645 desinfectant Substances 0.000 claims description 4
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 3
- 239000004576 sand Substances 0.000 claims description 3
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 3
- 230000035784 germination Effects 0.000 abstract description 23
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 230000006378 damage Effects 0.000 abstract description 2
- 238000002513 implantation Methods 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 36
- 239000003814 drug Substances 0.000 description 4
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- 244000296912 Ageratum conyzoides Species 0.000 description 1
- 235000004405 Ageratum conyzoides Nutrition 0.000 description 1
- 235000016535 Capraria biflora Nutrition 0.000 description 1
- 241000155170 Cymbaria mongolica Species 0.000 description 1
- 208000034507 Haematemesis Diseases 0.000 description 1
- 235000017309 Hypericum perforatum Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
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- 208000004880 Polyuria Diseases 0.000 description 1
- 206010053476 Traumatic haemorrhage Diseases 0.000 description 1
- 230000001139 anti-pruritic effect Effects 0.000 description 1
- 239000003908 antipruritic agent Substances 0.000 description 1
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- 239000008280 blood Substances 0.000 description 1
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- 238000007796 conventional method Methods 0.000 description 1
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- 239000012297 crystallization seed Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
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- 208000035861 hematochezia Diseases 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- KQPBSBAEBKRAAU-UHFFFAOYSA-N hypochlorous acid;sodium Chemical compound [Na].ClO KQPBSBAEBKRAAU-UHFFFAOYSA-N 0.000 description 1
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of method promoting core fragrant plant seed germination, belong to field of plant growing technology, the method relating to promoting plant germination by breeding and seedling technology, is a kind of method promoting core fragrant plant seed germination in particular.Core fragrant plant seed after the present invention will soak does destruction episperm and processes, and then carries out routine disinfection inoculation, constant temperature culture makes seed germination.Use method of the present invention can be effectively facilitated the sprouting of core fragrant plant seed, solve the problem that its germination is weak, germination percentage is low.The present invention also has feature simple to operate, that low cost, germination rate are high, easy to spread, and efficiently and effectively implantation in large scale, protection and sustainable utilization core fragrant plant resource are had the highest using value.
Description
Technical field
The invention belongs to field of plant growing technology, the method relating to promoting plant germination by breeding and seedling technology, more
A kind of specifically method promoting core fragrant plant seed germination.
Technical background
Mongolian medicine core fragrant plant, mongolian medicine name " my Tan-Ah giving ", is goatweed Da Wuli core fragrant plant Cymbariadahurica
L. with the dry herb of Radix Cymbariae mongolicae Cymbariamongolica Maxim., for Mongolian medicine's medicine dedicated.Originate in China Heilungkiang, interior
The hillslope that the provinces and regions such as Mongolia, Hebei are born between height above sea level 620-1100 rice with on gravel, grassland.Its mildly bitter flavor, cool in nature, have
Dry " Xieri Wusu Symptom ", heat clearing away, pain relieving, antipruritic, subside a swelling, the effect such as hemostasis." whole nation Chinese herbal medicine compilation " record core fragrant plant is dispeled the wind and removes
Wet, inducing diuresis to remove edema, effect of cooling blood for hemostasis, clinically be mainly used in treat arthromyodynia, double lower limbs edema, yellow fluid bleb, menorrhagia,
Haematemesis, epistaxis, have blood in stool, the disease such as traumatic hemorrhage.In recent years, due to the raising of core fragrant plant medical value, the market demand is continuously increased, and leads
Cause transition is excavated, and causes wild resource reserves increasingly to reduce, and resource is on the verge of exhaustion.In order to alleviate shortage of resources and exploitation
Between the contradiction that day by day increases, carrying out artificial growth is a kind of maximally effective approach.Preliminary breeding and seedling work finds,
Core fragrant plant exists in seeding process that germination percentage is low, the situation such as irregular of germinateing, and seriously governs standardized planting and the life of core fragrant plant
Produce.Therefore, extensive for core fragrant plant is cultivated and standardization production, and how research is shortened core fragrant plant seed germinating time, improved and plant
Sub-germination percentage becomes the technical problem that this area is urgently to be resolved hurrily.
Summary of the invention
It is an object of the invention to provide a kind of method that can promote core fragrant plant seed germination.
Technical solution of the present invention:
Core fragrant plant seed after the present invention will soak processes through destroying episperm, then carries out routine disinfection inoculation, constant temperature training
Support and make seed germination.
Further: core fragrant plant seed is avette for length, episperm yellowish-brown, and surface is more smooth and has gloss, and quality is softer, its
Mass of 1000 kernel is 865-885mg.
Further: the episperm of core fragrant plant seed is removed or at least cracks, but endotesta to be kept is the most impaired.
The temperature of constant temperature culture of the present invention is 25 DEG C, and humidity is 60.
Further: core fragrant plant seed episperm destruction processes and uses mechanical failure or chemical reagent to soak, and mechanical failure uses
Machine rolls or sand friction.
Because of habitat and the difference of kind, the Main Factors affecting seed germination also differs, and finds through on-the-spot investigation, impact
The dominant factor of seed germination is temperature, humidity, pericarp thickness, kernel maturing.Find through preliminary study, at core fragrant plant kind Ziye
In outer self-reproduction, pericarp thickness is the key factor of restriction core fragrant plant seed germination.Therefore core fragrant plant seed is destroyed by the present invention
Episperm processes.Find out the suitableeest method of core fragrant plant seed germination, it is provided that a kind of germination percentage is high, and germinating time is short, simple to operate
Core fragrant plant seed germination method, it is intended to provide necessary basic data for efficiently and effectively protection and sustainable utilization core fragrant plant resource.
What the method using promotion core fragrant plant seed germination of the present invention was had has the active effect that
(1) the effective time shortening core fragrant plant seed germination, solve that its germination is weak, the low problem of germination percentage；
(2) present invention also has feature simple to operate, that low cost, germination rate are high, easy to spread, to efficiently and effectively protecting
Protect and have the highest using value with sustainable utilization implantation in large scale core fragrant plant resource.
Detailed description of the invention
Experimental technique used in following embodiment if no special instructions, is conventional method.
Material used by following embodiment, reagent, if no special instructions, the most commercially reach.
Embodiment 1,
Core fragrant plant seed: pick up from Baotou periphery for planting experimentally son in the 6-7 month in 2014, be placed in paper envelopes
In, preserve under room temperature.
Seed selects: core fragrant plant seed is for long avette, and episperm yellowish-brown, surface is more smooth and has gloss, and quality is relatively
Soft.Its mass of 1000 kernel is 874.500 ± 4.186mg.We select full core fragrant plant seed that seed coat is complete as experiment material.
3. seed pre-treatment: be soaked in water 3-4 hour by the seed selected, after taking-up, covering of a seed keeps moistening, is divided into
Experimental group and matched group.
4. destroy episperm to process: use mechanical failure (machine rolls or sand friction) or chemical reagent to process (reagent
Soak) episperm of experimental group seed is removed, but endotesta to be kept is the most impaired.Matched group seed does not do this and processes.
5. sterilize and inoculate: before inoculation, Seed sterilization sterilization to be carried out, by the core fragrant plant seed of experimental group and matched group respectively
It is placed in the triangular flask after sterilizing, uses aseptic water washing seed.Add 20% hypochlorite disinfectant 7 ~ 8 min, discard hypochlorous acid
Sodium, adds 20% hypochlorite disinfectant 7 ~ 8 min.After sterilization, discard sodium hypochlorite, with rinsed with sterile water seed 3 times,
Each 5 min.
6. the investigation of seed germination germination percentage: spread one layer of absorbent cotton, upper cover one layer in the culture dish of a diameter of 9 cm
Filter paper, allows absorbent cotton and filter paper all suction moisture (sterilized water), is sprinkling upon uniformly on filter paper by the most each group seed, will plant as far as possible
Son is connected together but does not suffer.A metafiltration paper is covered again on seed.Each 50, every ware of process, is repeated 3 times, and is placed in 25 DEG C manually
Climate box is cultivated, keeps germinating bed to moisten.Every day observes, and every 24 h record 1 time.With plumule grow as seed length half time
Germination standard, 30 days is a cycle, and that adds up seed starts the natural law that germinates, germination percentage, germinating energy and germination index.
Germination percentage=(germination sum/for planting experimentally subnumber) × 100%；Germinating energy=(normal germination in germinating energy natural law
Subnumber is planted experimentally in grain number/confession) × 100%；Germination index (GI)=∑ (corresponding with Dt every day chitting piece number with germination number of days
Gt/ germination number of days Dt)
7. measurement result: through an experimental period (30 days), the seed of experimental group and matched group all has in various degree
Sprouting, experimental group seed started sprouting occur from the 4th day, and matched group seed starts sprouting occur from the 7th talent.One experiment
In cycle, the germination rate of experimental group seed is 93.63%, hence it is evident that higher than the 43.33% of matched group.
Core fragrant plant seed germination situation statistics (x=±s, (n=3))
Note: * represents compared with matched group, has pole significant difference in P=0.05 level.
Experiment conclusion:
(1) method for treating seeds used by the present invention can significantly shift to an earlier date the sprouting of core fragrant plant seed；
(2) germination percentage of the core fragrant plant seed after going episperm to process is apparently higher than the core fragrant plant percentage of seedgermination not processed
With germinating energy and germination index.
Claims (1)
1. the method promoting core fragrant plant seed germination, it is characterised in that core fragrant plant seed processes through destroying episperm, then carries out
Routine disinfection, inoculation, constant temperature culture make seed germination, and described episperm processes and uses mechanical failure or chemical reagent to soak, its
Middle mechanical failure uses machine to roll or sand friction, and the temperature of described constant temperature culture is 25 DEG C, and humidity is 60, before inoculation,
Seed sterilization sterilization to be carried out, adding mass concentration is 20% hypochlorite disinfectant 7 ~ 8 min, discards sodium hypochlorite, adds matter
Amount concentration is 20% hypochlorite disinfectant 7 ~ 8 min, after sterilization, discards sodium hypochlorite, rinses seed 3 times with water, each 5
min。
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| CN104472062B true CN104472062B (en) | 2016-11-23 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105165623B (en) * | 2015-10-12 | 2017-04-26 | 广西壮族自治区药用植物园 | Cymbaria mongolica maxim tissue culture breeding method |
| CN105145371B (en) * | 2015-10-12 | 2017-06-23 | 广西壮族自治区药用植物园 | A kind of Da Wuli cores fragrant plant tissue culture method for breeding |
| CN106489643A (en) * | 2016-10-19 | 2017-03-15 | 莱州市宏顺梅花种植科技有限公司 | A kind of Prunus mume var.bungo Makino breeding method |
| CN116870068A (en) * | 2023-08-07 | 2023-10-13 | 内蒙古科技大学包头医学院 | Application of shinyleaf yellowhorn leaf in preparation of medicine for preventing and treating hyperuricemia nephropathy |
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| CN101278650A (en) * | 2007-04-05 | 2008-10-08 | 上海市农业科学院园艺研究所 | Method for cultivating seedling and propagating tomato seeds |
| CN101451119A (en) * | 2008-12-26 | 2009-06-10 | 南京师范大学 | Phytolacca americana seed quick-speed germination method and its application in aseptic seedlings culture |
| CN101940158A (en) * | 2009-07-09 | 2011-01-12 | 正安县绿野科技产业有限责任公司 | Method for rapidly propagating plantlets by utilizing bletilla striata seeds |
| CN102487627B (en) * | 2011-12-09 | 2013-11-13 | 四川农业大学 | Method for effectively breaking dormancy of Paris polyphylla seeds |
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