CN103988608A - Method for promoting sprouting of rosa multibracteata seeds - Google Patents
Method for promoting sprouting of rosa multibracteata seeds Download PDFInfo
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- CN103988608A CN103988608A CN201410161284.2A CN201410161284A CN103988608A CN 103988608 A CN103988608 A CN 103988608A CN 201410161284 A CN201410161284 A CN 201410161284A CN 103988608 A CN103988608 A CN 103988608A
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Abstract
The invention discloses a method for promoting rosa multibracteata seeds to sprout. The method comprises the following steps including (1) rosa multibracteata fruit fermentation treatment; (2) seed drying in the air; (3) warm lamination: sphagna is sterilized in an autoclave sterilizer for 30 minutes, seeds are soaked for 24 hours in warm water and are mixed with the sterilized sphagna, meanwhile, sterile water is added, the sphagna mixed with the seeds is squeezed to a dry state by hand, the sphagna and the seeds are put into a reclosable bag, and the reclosable bag is placed into a 25 DEG C incubator for 8 to 16 weeks; (4) low-temperature lamination: the reclosable bag containing the sphagna and the seeds and subjected to warm lamination is placed into a 5 DEG C refrigerator, and the standing time is 4 to 16 weeks; and (5) seed sprouting. According to the method, measures of fruit fermentation, seed variable-temperature lamination and the like are adopted, the problems of low sprouting rate, long sprouting time and the like of the rosa multibracteata seeds are effectively solved, and the method has the advantages of simple process, low cost and the like. Through the implementation of the method, the sprouting rate is improved from 0 percent to 70 percents to 81.4 percents.
Description
Technical field
The invention belongs to breeding plant field, particularly a kind of method that promotes that how luxuriant Seeds of Rosa Spp germinates.
Background technology
Many luxuriant roses (Rosa multibracteata Hemsl. & E.H.Wilson) are rose family chamiso, are distributed in the provinces such as China Sichuan, Yunnan, height above sea level distribution 1200-2600 rice.Plant is up to 2.5 meters, and pollen is red, and 2-3 or several, flower becomes corymb, the florescence 5-7 month; Arnotto look, the fruit phase 7-10 month.Many luxuriant roses are a kind of important resource plants, active component (the old envelope political affairs that its plant contains energy Cardiovarscular, Zhao Weiquan, He Yonghua, Ding Lisheng, Wang Mingkui. the research of Rosa soulieana and how luxuriant rose chemical composition. application and environmental organism journal, 2000,6 (4): 334-336); Fruit contains the multiple nutrient component (He Yonghua useful to human body, Cao Yaling, Li Chaoluan, Pi Lidong. 22 kinds of Wild Rosa multiflora fruit Major Economics of China and important vitamin content. gardening journal, 1994,21 (2): 158-164), there is higher medical value and economic worth.This plant is also a kind of recovery species of important degraded ecosystem simultaneously, there is the significantly ecologic adaptation feature of drought-enduring and impoverishment tolerant, being distributed widely in Arid Valleys In Hengduan Mountainous Region, is important Indigenous Shrub Species (Zhou Zhiqiong, the Bao Weikai of dry valley revegetation, Wu Fuzhong, Pang Xueyong, He Xiao, Wu Ning. the how luxuriant rose growth and reproduction of arid valley of Minjiang River feature. Chinese Journal of Applied Ecology, 2007,18 (7): 1407-1413).
Different from cultivar, how luxuriant Wild Rosa multiflora is as very low in survival rate after the cuttages such as rose and Rosa soulieana, between 10%-20%; In addition, the scale of these vegetative propagation modes implements to be also subject to the restriction of cuttage or graft material.Therefore, Seeds of Rosa Spp breeding is all indispensable for aspects such as the breeding of new varieties, the recovery of Native species and the large-scale productions of cynarrhodion.But because Seeds of Rosa Spp has dormancy in various degree, general germination rate is low, particularly for the darker rose of part dormancy degree, usually time-consuming, weak effect of conventional low temperature lamination.Many luxuriant Seeds of Rosa Spps have compared with deep dormancy, and after traditional sulfuric acid corrosion, germination rate germination rate below 10%, after low temperature lamination, lower than 20%, survives the winter that it is low to bury rear germination rate, and sprouts irregular.Although carry out after sulfuric acid corrosion that cryosphere is long-pending can obtain higher germination rate (Zhou, Z.Q., Bao, W.K., & Wu, N. (2009) .Dormancy and germination of Rosa multibracteata.Scientia Horticulturae, 2009,119,434-441), but apply in production practices, not only cost is high, seedling quality is not good and easily cause environmental pollution.Up to the present, there is not yet by Patents or the reported in literature of the how luxuriant Seeds of Rosa Spp germination rate of non-chemically mass treatment raising.
Summary of the invention
The object of the invention: the germination rate that exists for how luxuriant Seeds of Rosa Spp after solving existing method and processing is low, sprout irregular problem, and a kind of method of the how luxuriant Seeds of Rosa Spp germination of promotion of economic environmental protection is provided.
Technical scheme: in order to realize foregoing invention object, a kind of method that promotes that how luxuriant Seeds of Rosa Spp germinates of the present invention is to carry out according to the following steps:
(1) rosaceous fruit fermentation process: be sprinkled into white wine (52 °) in the ripe rosaceous fruit of newly gathering, the amount of white wine is per kilogram fruit 10-15ml, in 20-60 DEG C of environment, cover after fermentation 5-10 days with plastic film, pericarp rotted, then with clear water by clean rosaceous fruit rinsing.
(2) seed is air-dry: by the Seeds of Rosa Spp after rinsing at temperature 10-25 DEG C, the 5-10 days that dries in the shade in air humidity 25-65% environment, then room temperature preservation 7-15 days.
(3) warm layer is long-pending: by for subsequent use after 30 minutes sphagna sterilizing in high-pressure sterilizing pot (108-121MP).Seed is soaked 24 hours in warm water (30-45 DEG C), mix with the sphagna after sterilizing, mixed proportion, sphagna: seed is 4:1(volume ratio).Add sterile water simultaneously, will mix seed-bearing sphagna hand and extract, until expressed water packs in valve bag again, be positioned over 25 DEG C of incubators, unglazed photograph, be 8-16 week standing time.Within every two weeks, open valve bag, ensure enough air, add 8-12ml sterile water simultaneously, keep sphagna and seed moistening.
(4) low temperature lamination: put into 5 DEG C of refrigerators by completing the long-pending valve bag that sphagna and seed are housed of warm layer, be 4-16 week standing time.Within every two weeks, open valve bag, ensure enough air, add the sterile water of 8-12ml simultaneously, keep sphagna and seed moistening.
(5) seed germination: the seed of breaking dormancy is separated from sphagna, put into the culture dish that two-layer moistening filter paper is housed, sprout in 20 DEG C of incubators.
The abundant rinsing of the clear water rose pulp that rots for seed in described step (1), and soak seed 8-12 hour with clear water.
In the long-pending and low temperature lamination process of described step (3) and the warm layer of (4), within every two weeks, open valve bag 3-5 minute, ensure enough air, add the sterile water of 8-12ml simultaneously, keep sphagna and seed moistening.
Embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is described in further detail.Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
Embodiment mono-
On October 3rd, 2007 gathers ripe rosaceous fruit 1kg in Jing Zhou village, Mao County, splashes into white wine (52 °) 10-15ml, in 20-60 DEG C of environment, covers after fermentation 5-10 days with plastic film, and pericarp is rotted, then with clear water by clean rosaceous fruit rinsing.
1) by the Seeds of Rosa Spp after rinsing at temperature 10-25 DEG C, the 5-10 days that dries in the shade in air humidity 25-65% environment, room temperature preservation 7-15 days.
2) by for subsequent use after 30 minutes sphagna sterilizing in high-pressure sterilizing pot (108-121MP).Seed is soaked 24 hours in warm water (30-45 DEG C), mix with the sphagna after sterilizing, mixed proportion, sphagna: seed is 4:1(volume ratio).Add sterile water simultaneously, will mix seed-bearing sphagna hand and extract, until expressed water comes again, then pack in valve bag, be positioned over 25 DEG C of incubators, unglazed photograph, be 8 weeks standing time.Within every two weeks, open valve bag, ensure enough air, add 8-12ml sterile water simultaneously, keep sphagna and seed moistening.
3) put into 5 DEG C of refrigerators by completing the long-pending valve bag that sphagna and seed are housed of warm layer, be 4 weeks standing time.Within every two weeks, open valve bag, ensure enough air, add 8-12ml sterile water simultaneously, keep sphagna and seed moistening.
4) culture dish and filter paper are shifted to an earlier date high-pressure sterilizing pot (108-121MP) sterilizing 30 minutes, spread double-deck filter paper in culture dish bottom, wetting with 8-10ml sterile water.Seed after treatment is separated from sphagna, and 10% liquor natrii hypochloritis sterilizes 10 minutes, then selects 50 seed intervals to arrange uniformly on culture dish filter paper, and 3 repetitions, water and have ponding to surface but immobilising state.Then culture dish is put in incubator to 20 DEG C of temperature, 16h illumination/10h.Add up germination rate every day, and water in right amount.
Embodiment bis-
1) gather ripe rosaceous fruit 1kg in Jing Zhou village, Mao County on October 3rd, 2007, splash into white wine (52 °) 10-15ml, in 20-60 DEG C of environment, cover after fermentation 5-10 days with plastic film, pericarp is rotted, then with clear water by clean rosaceous fruit rinsing;
2) by the Seeds of Rosa Spp after rinsing at temperature 10-25 DEG C, the 5-10 days that dries in the shade in air humidity 25-65% environment, room temperature preservation 7-15 days.
3) by for subsequent use after 30 minutes sphagna sterilizing in high-pressure sterilizing pot (108-121MP).Seed is soaked 24 hours in warm water (30-45 DEG C), mix with the sphagna after sterilizing, mixed proportion, sphagna: seed is 4:1(volume ratio).Add sterile water simultaneously, will mix seed-bearing sphagna hand and extract, until expressed water comes again, then pack in valve bag, be positioned over 25 DEG C of incubators, unglazed photograph, be 16 weeks standing time.Within every two weeks, open valve bag, ensure enough air, add 8-12ml sterile water simultaneously, keep sphagna and seed moistening.
4) put into 5 DEG C of refrigerators by completing the long-pending valve bag that sphagna and seed are housed of warm layer, be 8 weeks standing time.Within every two weeks, open valve bag, ensure enough air, add 8-12ml sterile water simultaneously, keep sphagna and seed moistening.
5) culture dish and filter paper are shifted to an earlier date (108-121MP) lower sterilizing 30 minutes, spread double-deck filter paper in culture dish bottom, wetting with sterile water.Seed after treatment is separated from sphagna, and 10% liquor natrii hypochloritis sterilizes 10 minutes, then selects 50 seed intervals to arrange uniformly on culture dish filter paper, and 3 repetitions, water and have ponding to surface but immobilising state.Then culture dish is put in incubator to 20 DEG C of temperature, 16h illumination/10h.Add up germination rate every day, and water in right amount.
Embodiment tri-
1) gather ripe rosaceous fruit 1kg in Jing Zhou village, Mao County on October 3rd, 2007, splash into white wine (52 °) 10-15ml, in 20-60 DEG C of environment, cover after fermentation 5-10 days with plastic film, pericarp is rotted, then with clear water by clean rosaceous fruit rinsing;
2) by the Seeds of Rosa Spp after rinsing at temperature 10-25 DEG C, the 5-10 days that dries in the shade in air humidity 25-65% environment, room temperature preservation 7-15 days.
3) by for subsequent use after 30 minutes sphagna sterilizing in high-pressure sterilizing pot (108-121MP).Seed is soaked 24 hours in warm water (30-45 DEG C), mix with the sphagna after sterilizing, mixed proportion, sphagna: seed is 4:1(volume ratio).Add sterile water simultaneously, will mix seed-bearing sphagna hand and extract, until expressed water comes again, then pack in valve bag, be positioned over 25 DEG C of incubators, unglazed photograph, be 8 weeks standing time.Within every two weeks, open valve bag, ensure enough air, add 8-12ml sterile water simultaneously, keep sphagna and seed moistening.
4) put into 5 DEG C of refrigerators by completing the long-pending valve bag that sphagna and seed are housed of warm layer, be 16 weeks standing time.Within every two weeks, open valve bag, ensure enough air, add 8-12ml sterile water simultaneously, keep sphagna and seed moistening.
5) culture dish and filter paper are shifted to an earlier date (108-121MP) lower sterilizing 30 minutes, spread double-deck filter paper in culture dish bottom, wetting with sterile water.Seed after treatment is separated from sphagna, and 10% liquor natrii hypochloritis sterilizes 10 minutes, then selects 50 seed intervals to arrange uniformly on culture dish filter paper, and 3 repetitions, water and have ponding to surface but immobilising state.Then culture dish is put in incubator to 20 DEG C of temperature, 16h illumination/10h.Add up germination rate every day, and water in right amount.
Embodiment tetra-
1) gather ripe rosaceous fruit 1kg in Jing Zhou village, Mao County on October 3rd, 2007, splash into white wine (52 °) 10-15ml, in 20-60 DEG C of environment, cover after fermentation 5-10 days with plastic film, pericarp is rotted, then with clear water by clean rosaceous fruit rinsing;
2) by the Seeds of Rosa Spp after rinsing at temperature 10-25 DEG C, the 5-10 days that dries in the shade in air humidity 25-65% environment, room temperature preservation 7-15 days.
3) by for subsequent use after 30 minutes sphagna sterilizing in high-pressure sterilizing pot (108-121MP).Seed is soaked 24 hours in warm water (30-45 DEG C), mix with the sphagna after sterilizing, mixed proportion, sphagna: seed is 4:1(volume ratio).Add sterile water simultaneously, will mix seed-bearing sphagna hand and extract, until expressed water comes again, then pack in valve bag, be positioned over 25 DEG C of incubators, unglazed photograph, be 16 weeks standing time.Within every two weeks, open valve bag, ensure enough air, add 8-12ml sterile water simultaneously, keep sphagna and seed moistening.
4) put into 5 DEG C of refrigerators by completing the long-pending valve bag that sphagna and seed are housed of warm layer, be 16 weeks standing time.Within every two weeks, open valve bag, ensure enough air, add 8-12ml sterile water simultaneously, keep sphagna and seed moistening.
5) culture dish and filter paper are shifted to an earlier date (108-121MP) lower sterilizing 30 minutes, spread double-deck filter paper in culture dish bottom, wetting with sterile water.Seed after treatment is separated from sphagna, and 10% liquor natrii hypochloritis sterilizes 10 minutes, then selects 50 seed intervals to arrange uniformly on culture dish filter paper, and 3 repetitions, water and have ponding to surface but immobilising state.Then culture dish is put in incubator to 20 DEG C of temperature, 16h illumination/10h.Add up germination rate every day, and water in right amount.
Comparative trial:
Design respectively seven kinds of processing modes improve germination rate processing to seed, each processing arranges 3 repetitions, 50 seeds of each repetition.Rosaceous fruit fermentation process, seed air-dry and process after the environmental condition of seed germination consistent with embodiment.
Experimental design: contrast, be left intact, the air-dry rear direct germination of seed.Process one: seed is air-dry, 98% concentrated sulfuric acid corrosion 6 hours.Process two: seed is air-dry, low temperature lamination 8 weeks.Process three: seed is air-dry, low temperature lamination 12 weeks.Process four: seed is air-dry, low temperature lamination 16 weeks.Process five: seed is air-dry, low temperature lamination 20 weeks.Process six: seed is air-dry, low temperature lamination 24 weeks.Running water rinses to neutrality and carries out seed germination.
The parameter of assessment seed germination:
Germination rate=germinative number/full seed number
Germination vigor: first 8 days seed sprouting numbers account for the percentage of full seed number
T50: reach the final required time of germination rate 50%
Experimental implementation and result:
20 DEG C of temperature, 14h illumination, sprouts in the incubator of 10h dark, observes sprouting situation every day, and its result is as follows:
| ? | Germination rate (%) | Germination vigor (%) | T50(days) |
| Contrast | 0 | 0 | --- |
| Process one | 0 | 0 | --- |
| Process two | 0 | 0 | --- |
| Process three | 0 | 0 | --- |
| Process four | 5.7 | 0 | 12.7 |
| Process five | 11.7 | 4.5 | 11.0 |
| Process six | 13.8 | 13.8 | 13.8 |
| Embodiment mono- | 22.7 | 6.5 | 9.3 |
| Embodiment bis- | 81.4 | 74.2 | 4.3 |
| Embodiment tri- | 70.0 | 60.3 | 5.3 |
| Embodiment tetra- | 57.6 | 22.1 | 4.3 |
Claims (3)
1. the method that the how luxuriant Seeds of Rosa Spp of promotion germinates, is characterized in that being made up of following steps:
(1) rosaceous fruit fermentation process: in the ripe rosaceous fruit of newly gathering, splash into white wine (52%), in 20-60 DEG C of environment, cover after fermentation 5-10 days with plastic film, pulp is rotted, then with clear water by clean the rinsing of rose pulp;
(2) seed is air-dry: by the Seeds of Rosa Spp after rinsing at 10 DEG C-25 DEG C of temperature, the 5-10 days that dries in the shade in air humidity 25-65% environment, room temperature preservation 7-15 days.
(3) warm layer is long-pending: by for subsequent use after 30 minutes sphagna sterilizing in high-pressure sterilizing pot (105 DEG C, 102MP).Seed is soaked 24 hours in warm water (30-45 DEG C), mix with the sphagna after sterilizing, mixed proportion, sphagna: seed is 4:1(volume ratio), add sterile water simultaneously, will mix seed-bearing sphagna hand and extract, pack in valve bag, be positioned over 25 DEG C of incubators, unglazed photograph, be 8-16 week standing time.
(4) low temperature lamination: put into 5 DEG C of refrigerators by completing the long-pending valve bag that sphagna and seed are housed of warm layer, be 4-16 week standing time.
(5) seed germination: the seed of breaking dormancy is separated from sphagna, put into the culture dish that two-layer moistening filter paper is housed, sprout in 20 DEG C of incubators.
2. a kind of method that promotes the sub-seed sprouting of how luxuriant rose according to claim 1, is characterized in that in step (1) the abundant rinsing of the seed clear water rose pulp that rots, and soaks seed 8-12 hour with clear water.
3. a kind of method that promotes the sub-seed sprouting of how luxuriant rose according to claim 1, it is characterized in that in and low temperature lamination process long-pending at the warm layer of step (3) and (4), within every two weeks, open valve bag 3-5 minute, ensure enough air, add 8-12ml sterile water simultaneously, keep sphagna and seed moistening.
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Cited By (8)
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| CN105123029A (en) * | 2015-07-24 | 2015-12-09 | 句容市满园春家庭农场 | Seed processing method of malus halliana |
| CN106576493A (en) * | 2016-11-28 | 2017-04-26 | 全椒凤巢植保专业合作社 | Fast germination accelerating method for crape myrtle seeds |
| CN108934275A (en) * | 2018-07-10 | 2018-12-07 | 云南省林业科学院 | A kind of bog moss storing and germination accelerating method |
| CN109197019A (en) * | 2018-10-29 | 2019-01-15 | 南京林业大学 | A method of breaking snowball seed dormancy |
| CN111543137A (en) * | 2020-05-30 | 2020-08-18 | 江苏嘉润生态苗木有限公司 | Rose seed seedling raising method |
| CN112603842A (en) * | 2020-12-31 | 2021-04-06 | 中国科学院成都生物研究所 | Active rose extract and application thereof |
| CN114258757A (en) * | 2021-12-31 | 2022-04-01 | 株洲千金药业股份有限公司 | Germination accelerating method for rose seeds |
| CN114451094A (en) * | 2022-01-21 | 2022-05-10 | 中国科学院昆明植物研究所 | Method for promoting germination of rose seeds |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105123029A (en) * | 2015-07-24 | 2015-12-09 | 句容市满园春家庭农场 | Seed processing method of malus halliana |
| CN106576493A (en) * | 2016-11-28 | 2017-04-26 | 全椒凤巢植保专业合作社 | Fast germination accelerating method for crape myrtle seeds |
| CN106576493B (en) * | 2016-11-28 | 2017-11-14 | 全椒凤巢植保专业合作社 | A kind of crape myrtle seed quick pregermination method |
| CN108934275A (en) * | 2018-07-10 | 2018-12-07 | 云南省林业科学院 | A kind of bog moss storing and germination accelerating method |
| CN109197019A (en) * | 2018-10-29 | 2019-01-15 | 南京林业大学 | A method of breaking snowball seed dormancy |
| CN111543137A (en) * | 2020-05-30 | 2020-08-18 | 江苏嘉润生态苗木有限公司 | Rose seed seedling raising method |
| CN111543137B (en) * | 2020-05-30 | 2022-07-05 | 江苏嘉润生态苗木有限公司 | Rose seed seedling raising method |
| CN112603842A (en) * | 2020-12-31 | 2021-04-06 | 中国科学院成都生物研究所 | Active rose extract and application thereof |
| CN114258757A (en) * | 2021-12-31 | 2022-04-01 | 株洲千金药业股份有限公司 | Germination accelerating method for rose seeds |
| CN114451094A (en) * | 2022-01-21 | 2022-05-10 | 中国科学院昆明植物研究所 | Method for promoting germination of rose seeds |
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