CN101120635A - Method for promoting nitraria seed quick germination - Google Patents
Method for promoting nitraria seed quick germination Download PDFInfo
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- CN101120635A CN101120635A CNA2007100186975A CN200710018697A CN101120635A CN 101120635 A CN101120635 A CN 101120635A CN A2007100186975 A CNA2007100186975 A CN A2007100186975A CN 200710018697 A CN200710018697 A CN 200710018697A CN 101120635 A CN101120635 A CN 101120635A
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Abstract
The present invention primarily relates to a method promoting the germination of the Nitraria schoberi seed. A method promotes the rapid germination of the Nitraria schoberi seed comprises the following procedures of A. the seed of Nitraria schoberi is collected, the fruit pulp on the surface of the seed is removed, the seed is dried after being washed in the water in normal temperature and finally the seed is stored in the temperature of twenty to forty centigrade degrees below zero to be prepared for use; B. after immersed in a sulfuric acid solution with a density of thirty to ninety eight percentages for half an hour to 24 hours, the seed of Nitraria schoberi seed is washed by the water to have the pH value between six and eight; the seed is sterilized in an alcohol solution of 50 percentages to 100 percentages about one to thirty minutes, the seed is washed for four to six times; under the condition of the temperature range between fifteen and forty centigrade degrees, the seed is immersed in the water for 24 to 72 hours and the seed is arranged on the seed bed for sprouting under the same temperature condition. The present invention is characterized in that the method can rapidly relieve the dormancy of the Nitraria schoberi seed in a high efficiency and can make the germination rate of the seed reach 69 percentages within ten days; moreover, the germination is orderly, which greatly save the time for breeding the seedling or breeding the seed of Nitraria schoberi.
Description
Technical field:
The present invention relates generally to the method that promotes white thorn seed germination.
Background technology:
White thorn (Nitraria tangutorum) belongs to zygophyllaceae, it is the valuable source plant that is distributed in the NORTHWEST CHINA area, integrate check winds and fix drifting sand, purposes such as water and soil conservation, alkaline land improving, herbage and medicinal material, therefore, be the resource plant that desert and saline and alkaline area are worth greatly developing.
White thorn seed dormancy rate height has seriously limited research and the utilization of people to this good species.Forefathers' germination accelerating method of dialogue thorn seed have carried out the research of many trials, and have obtained certain achievement.These germination accelerating methods can be classified as 4 classes generally, promptly husky Tibetan vernalization, temperature are hidden vernalization, water logging vernalization and lamination vernalization.
Husky Tibetan vernalization will be stung seed in vain before this and will be soaked in water, mix in husky and 3: 1 ratio of seed, and with the band mouthful plastic wraps get up, be positioned over leeward area without shade vernalization, keep moistening, often stir up and down, seed showed money or valuables one carries unintentionally in general 30 days, treated that 1/3 seed splits mouth and shows money or valuables one carries unintentionally and can sow, but the few of germination rate report arranged, only have sowing after 14 days emergence rate be 10%, and remaining all undeclared germination or the situation of emerging.Though use in addition with a kind of germination accelerating method, in concrete operations, still have certain difference, kind of disinfectant etc. has difference during as water temperature when seed is soaked and seed disinfection.
Need to be covered with when piling up vernalization straw bag when temperature is hidden vernalization, other all is same as the husky vernalization of hiding.After the method vernalization, in green house, sow.
Water logging vernalization is the seed cold water soak, changes water every day 2 times, keeps the seed ventilation, soak seed after 10 days, and sowing in the green house, general 7-11d cotyledon is unearthed.
Lamination vernalization is with after puddling with moistening river sand layering behind 40-50 ℃ the emerge in worm water 48-72h, between at the beginning of 2 months mid-December to next year then, carry out the about 2-3 of low temperature lamination processing after the month, mid-April with the seed of handling be positioned on the sunny side, the draughty face of land, carry out change temperature-accelerated germination.The covering with ventilation sunshade is placed on the seed top layer of vernalization, and stir 1-2 every day, looks and does wet situation water spray.Under temperature went up very fast condition, seed 3-7d can split mouth and show money or valuables one carries unintentionally.
Although forefathers have obtained certain progress aspect the white thorn seed dormancy breaking, but these presprouting of seeds technology are time-consuming, effort, in addition as temperature, water is gentle etc., and condition control is difficult to get a desired effect, and be awkward in the laboratory, more important is that germination rate is low.
Summary of the invention:
The objective of the invention is to overcome the deficiencies in the prior art part,, further improve white thorn seed germination rate, shorten white thorn greatly and grow seedlings or breeding time for production practices provide a kind of method that promotes nitraria seed quick germination; Help the research of scientific worker's dialogue thorn Physiology and biochemistry and molecular biology thereof etc., for the white thorn of better utilization resource plant is from now on laid a solid foundation.
Purpose of the present invention can be by realizing by the following technical solutions: a kind of method that promotes nitraria seed quick germination includes following steps:
A. gather white thorn seed, remove the surface of the seed pulp, with drying after the normal-temperature water washing, in that (20) ℃-preservation is standby down for (40) ℃ temperature condition;
B. will sting seed in vain is the H of 30-98% through concentration
2SO
4After soaking 0.5-24h, water wash to the pH value between 6-8, place the about 1-30min of alcohol sterilization of 50-100%, water flushing is 4-6 time again, is between 15-40 ℃ under the condition, behind the 24-72h that is soaked in water earlier in temperature range, place on kind of the bed, germinate down in the same temperature condition again.
The method of described promotion nitraria seed quick germination, after the water of in the alcohol of B step, sterilizing the again flushing, carry out the alternating temperature of high and low temperature alternative and handle any point of high temperature between 20-40 ℃, the any point of low temperature between 0-15 ℃, and high low temperature handling duration is all between 8h-24h.
The method of described promotion nitraria seed quick germination, also including at B step alcohol disinfecting again after the water flushing, is the gibberellin bubble kind 24-72h of 50-500mg/L with concentration range.
The method of described promotion nitraria seed quick germination also includes at the germination temperature on kind of the bed of placing described in the B step and also includes any point constant temperature between 15-40 ℃.
The method of described promotion nitraria seed quick germination also includes mercury chloride or potassium permanganate or the clorox sterilization 1-30min that uses 0.1-10% in the seed disinfection of B step dialogue thorn.
The method of described promotion nitraria seed quick germination, described kind bed are culture dish or the husky bed that is covered with filter paper.
The invention has the beneficial effects as follows, at normal temperatures, use the distilled water presoaking and germinating, dialogue stings the sprouting of seed without any influence, and the germination rate in 10 days is 0%; And with the H of 95-98%
2SO
4Behind bubble kind of the 55min, under the condition of 25/5 ℃ of (12h/12h) alternating temperature and lucifuge, through 150mg/L gibberellin (GA
3) soak 48h, can rapidly and efficiently abolish white thorn seed dormancy and make its germination rate in 10 days reach 69%, and it is neat to germinate, saved greatly that white thorn is grown seedlings or the time of breeding.Tentative confirmation stings seed in vain and belongs to comprehensive resting form, and the germination inhibitor that the machinery when its kind skin extends seed base-root hinders and seed contains is to cause the seed dormant main cause of white thorn.
Description of drawings:
Each H of Fig. 1
2 S
O4 bubbles kind of duration dialogue stings the schematic diagram that influences of potentiality of seed.
Each H of Fig. 2
2SO
4Bubble kind of duration dialogue stings the schematic diagram that influences of percentage of seedgermination.
Fig. 3 H
2SO
4The germination vigor schematic diagram of the down white thorn seed of different extent of alternating temperature behind bubble kind of the 55min.
Fig. 4 H
2SO
4The down white thorn seed germination rate of different extent of alternating temperature schematic diagram behind bubble kind of the 55min.
Fig. 5 H
2SO
4Behind bubble kind of the 55min under 25/5 ℃ of alternating temperature condition the gibberellin treatment dialogue thorn potentiality of seed of variable concentrations influence schematic diagram.
Fig. 6 H
2SO
4Behind bubble kind of the 55min under 25/5 ℃ of alternating temperature condition the gibberellin treatment dialogue thorn percentage of seedgermination of variable concentrations influence schematic diagram.
Embodiment:
Below in conjunction with shown in most preferred embodiment be described in further detail:
Embodiment 1: a kind of method that promotes nitraria seed quick germination includes following steps:
A. gather white thorn seed, remove the surface of the seed pulp,, preserve standby down at-7 ℃ with drying after the running water washing;
B. will sting seed in vain is respectively the H of 95-98% through concentration
2SO
4Behind immersion 45,50,55 and the 60min, with distilled water flushing to the pH value between 6.5-7, place 90% the about 3min of alcohol sterilization, use distilled water flushing again 5 times, at preset temperature is 25 ℃, illumination be in the incubator of 0lx earlier with behind the distillation bubble kind 48h, place on the kind bed of the culture dish that is covered with double-deck filter paper, put back at last in the incubator of the same setting and germinate.
Its effect is:
H
2SO
4The influence of bubble kind of dialogue thorn seed germination:
Control group distilled water presoaking and germinating wherein.
Fig. 1 shows, the H of control group and 95-98%
2SO
4Each white thorn potentiality of seed of handling of bubble kind 45,50,55 and 60min is respectively 0%, 7%, 14%, 22% and 16%.
Fig. 2 shows, the H of control group and 95-98%
2SO
4Each handles bubble kinds 45,50,55 and 60min white thorn percentage of seedgermination and is respectively 0%, 11%, 19%, 26% and 20%.
Embodiment 2: a kind of method that promotes nitraria seed quick germination includes following steps:
A. gather white thorn seed, remove the surface of the seed pulp,, preserve standby down at-7 ℃ with drying after the running water washing;
B. will sting seed in vain is the H of 95-98% through concentration
2SO
4After soaking 55min, with distilled water flushing to the pH value between 6.5-7, place the about 3min of 90% alcohol sterilization, use distilled water flushing again 5 times, be respectively after 25 ℃ of constant temperature and 25/5 ℃, 25/10 ℃, 25/15 ℃ (12h/12h) alternating temperature and illumination are in four incubators of 0lx with distillation bubble kind 48h at preset temperature, place on the kind bed of the culture dish that is covered with double-deck filter paper, put back to separately at last in the incubator of the same setting and germinate.
Its effect is:
The H of 95-98%
2SO
4Behind bubble kind of the 55min, different extent of alternating temperature are to abolishing the seed dormant influence of white thorn:
Fig. 3 shows, through the H of 95-98%
2SO
4Behind bubble kind of the 55min, under the processing of 25 ℃ of constant temperature and 25/5 ℃, 25/10 ℃, 25/15 ℃ (12h/12h) alternating temperature, the germination vigor that stings seed in vain is respectively 22%, 20%, 19% and 22%.Show through variance analysis, although each alternating temperature handle handle with 25 ℃ of constant temperature between and the germination vigor of each alternating temperature between handling all do not have significant difference (P＞0.05), 25/5 ℃ and 25/10 ℃ of alternating temperature handle that to sting seed sprouting down in vain more neat.
Fig. 4 shows, through the H of 95-98%
2SO
4Behind the seed soaking 55min, under the processing of 25 ℃ of constant temperature and 25/5 ℃, 25/10 ℃, 25/15 ℃ (12h/12h) alternating temperature, white thorn seed germination rate is respectively 26%, 27%, 25% and 23%, between although each alternating temperature is handled and is handled with 25 ℃ of constant temperature and the germination rate between each alternating temperature processing all do not have significant difference (P＞0.05), but germination rate is the highest when 25/5 ℃ of alternating temperature as can be seen, and to handle the germination of stinging seed down in vain more neat for 25/5 ℃ and 25/10 ℃ of alternating temperature simultaneously.
Embodiment 3: a kind of method that promotes nitraria seed quick germination includes following steps:
A. gather white thorn seed, remove the surface of the seed pulp,, preserve standby down at-7 ℃ with drying after the running water washing;
B. will sting seed in vain is the H of 95-98% through concentration
2SO
4After soaking 55min, water wash to the pH value between 6.5-7, place 90% the about 3min of alcohol sterilization, the water flushing is 6 times again, at default alternating temperature is that 25/15 ℃ (12h/12h) and illumination are in the incubator of 0lx, respectively with 50,100,150 and the gibberellin of 200mg/L bubble kind of 48h after, place on the kind bed of the culture dish that is covered with double-deck filter paper, put back at last in the incubator of the same setting and germinate.
Its effect is:
The H of 95-98%
2SO
4Bubble kind of a 55min, under the condition that 25/5 ℃ of alternating temperature is handled, the influence of variable concentrations gibberellin seed soaking dialogue thorn seed germination:
Fig. 5 shows, through the H of 95-98%
2SO
4Behind bubble kind of the 55min, under 25/5 ℃ of alternating temperature condition, contrast and through 50,100,150 and the potentiality of seed of processing such as 200mg/L gibberellin bubble kind of 48h be respectively 20%, 22%, 35%, 48% and 41%.
As shown in Figure 6, through the H of 95-98%
2SO
4Behind bubble kind of the 55min, under 25/5 ℃ of alternating temperature condition, contrast and through 50,100,150 and the percentage of seedgermination of processing such as 200mg/L gibberellin bubble kind of 48h be respectively 27%, 33%, 45%, 69% and 57%.
Embodiment 4: a kind of method that promotes nitraria seed quick germination includes following steps:
A. gather white thorn seed, remove the surface of the seed pulp,, under normal temperature condition, preserve standby with drying after the normal-temperature water washing;
B. will sting seed in vain is the H of 30-35% through concentration
2SO
4After soaking 24h, water wash to the pH value between 7-7.5, place 0.6% potassium permanganate sterilization 20min, the water flushing is 4-6 time again, places on the culture dish kind bed with behind the bubble kind 48h earlier under normal temperature condition, germinates under normal temperature condition.
Embodiment 5: a kind of method that promotes nitraria seed quick germination includes following steps:
A. gather white thorn seed, remove the surface of the seed pulp,, under-10 ℃ condition, preserve standby with drying after the running water washing;
B. will sting seed in vain is the H of 40-45% through concentration
2SO
4After soaking 16h, water washes to pH value 7.5-8, places 5% clorox sterilization 20min, the water flushing is 5 times again, behind gibberellin bubble kind of the 24h that under the condition of normal temperature lucifuge with concentration is 500mg/L earlier, place on the husky bed, under normal temperature and lucifuge condition, germinate.
Embodiment 6: a kind of method that promotes nitraria seed quick germination includes following steps:
A. gather white thorn seed, remove the surface of the seed pulp,, under-15 ℃ condition, preserve standby with drying after the normal-temperature water washing;
B. will sting seed in vain is the H of 50-55% through concentration
2SO
4After soaking 8h, water wash to the pH value between 7.5-8, place 0.3% mercury chloride sterilization 5min, the water flushing is 6 times again, preset temperature be 25 ℃ and illumination be in the incubator of 0lx earlier with bubble kind 36h after, place on the husky bed, put back at last in the incubator of the same setting and germinate.
Embodiment 7: a kind of method that promotes nitraria seed quick germination includes following steps:
A. gather white thorn seed, remove the surface of the seed pulp,, under-20 ℃ condition, preserve standby with drying after the normal-temperature water washing;
B. will sting seed in vain is the H of 60-65% through concentration
2SO
4After soaking 6h, water wash to the pH value between 7-7.5, place 1% mercury chloride sterilization 3min, the water flushing is 4 times again, be respectively after 40/15 ℃ of (24h/24h) alternating temperature and intensity of illumination be in the incubator of 0lx with bubble kind 72h at preset temperature, place on the culture dish kind bed, put back at last in the incubator of the same setting and germinate.
Embodiment 8: a kind of method that promotes nitraria seed quick germination includes following steps:
A. gather white thorn seed, remove the surface of the seed pulp,, under-15 ℃ condition, preserve standby with drying after the normal-temperature water washing;
B. will sting seed in vain is the H of 70-75% through concentration
2SO
4After soaking 4h, water wash to the pH value between 6.5-7, place 1% potassium permanganate sterilization 15min, the water flushing is 4 times again, be respectively after 15/1 ℃ of (8h/8h) alternating temperature and illumination is in the incubator of 2000lx with bubble kind 72h at preset temperature, place on the culture dish kind bed, put back at last in the incubator of the same setting and germinate.
Embodiment 9: a kind of method that promotes nitraria seed quick germination includes following steps:
A. gather white thorn seed, remove the surface of the seed pulp,, under-15 ℃ condition, preserve standby with drying after the normal-temperature water washing;
B. will sting seed in vain is the H of 80-85% through concentration
2SO
4After soaking 2h, water wash to the pH value between 6.5-7, place 5% clorox sterilization 15min, the water flushing is 6 times again, preset temperature be respectively in the incubator that 35/10 ℃ of (8h/16h) alternating temperature and illumination are 4000lx with concentration be the gibberellin bubble kind 72h of 100mg/L earlier after, place on the culture dish kind bed, put back at last in the incubator of the same setting and germinate.
Claims (7)
1. method that promotes nitraria seed quick germination includes following steps:
A. gather white thorn seed, remove the surface of the seed pulp, with drying after the normal-temperature water washing, preservation is standby down for the temperature condition in (20) ℃-(40) ℃;
B. will sting seed in vain is the H of 30-98% through concentration
2SO
4After soaking 0.5-24h, water wash to the pH value between 6-8, place the about 1-30min of alcohol sterilization of 50-100%, water flushing is 4-6 time again, under temperature range is condition between 15-40 ℃, behind the 24-72h that is soaked in water earlier, place on kind of the bed, under the same temperature condition, germinate again.
2. the method for promotion nitraria seed quick germination as claimed in claim 1, after the water that it is characterized in that in the alcohol of B step, sterilizing the again flushing, carrying out the alternating temperature of high and low temperature alternative handles, the any point of high temperature between 20-40 ℃, the any point of low temperature between 0-15 ℃, and high low temperature handling duration is all between 8h-24h.
3. the method for promotion nitraria seed quick germination as claimed in claim 1 or 2, it is characterized in that also including at B step alcohol disinfecting again after the water flushing, is the gibberellin bubble kind 24-72h of 50-500mg/L with concentration range.
4. the method for promotion nitraria seed quick germination as claimed in claim 1 or 2 is characterized in that also including at the germination temperature on kind of the bed of placing described in the B step and also includes any point constant temperature between 15-40 ℃.
5. the method for promotion nitraria seed quick germination as claimed in claim 3 is characterized in that also including at the germination temperature on kind of the bed of placing described in the B step and also includes any point constant temperature between 15-40 ℃.
6. the method for promotion nitraria seed quick germination as claimed in claim 1 is characterized in that also including mercury chloride or potassium permanganate or the clorox sterilization 1-30min that uses 0.1-10% in the seed disinfection of B step dialogue thorn.
7. the method for promotion nitraria seed quick germination as claimed in claim 1 is characterized in that described culture dish or the husky bed of bed for being covered with filter paper of planting.
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| CN100531552C CN100531552C (en) | 2009-08-26 |
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| CN110226440A (en) * | 2018-11-07 | 2019-09-13 | 河西学院 | A kind of Nitraria tangutorum open country method for culturing seedlings |
| CN110226440B (en) * | 2018-11-07 | 2022-04-29 | 河西学院 | Method for growing seedlings of Nitraria tangutorum bobr in open field |
| CN111972075A (en) * | 2020-09-16 | 2020-11-24 | 西北民族大学 | Method for breaking dormancy of jerusalem artichoke seeds and promoting germination |
| CN111972075B (en) * | 2020-09-16 | 2022-04-26 | 西北民族大学 | Method for breaking dormancy of jerusalem artichoke seeds and promoting germination |
| CN112243634A (en) * | 2020-11-04 | 2021-01-22 | 内蒙古自治区阿拉善盟林业治沙研究所(阿拉善盟林业调查规划设计队) | Method for promoting nitraria tangutorum bobr seeds to germinate rapidly |
| CN114568135A (en) * | 2022-03-25 | 2022-06-03 | 成都中医药大学 | Preservation method and germination method of evergreen fruit tree seeds |
| CN114568135B (en) * | 2022-03-25 | 2023-10-20 | 成都中医药大学 | Preservation method and germination method of evergreen fruit tree seeds |
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