CN102119594B - Method for promoting rhododendron parvifolium seed germination - Google Patents
Method for promoting rhododendron parvifolium seed germination Download PDFInfo
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- CN102119594B CN102119594B CN201010584944A CN201010584944A CN102119594B CN 102119594 B CN102119594 B CN 102119594B CN 201010584944 A CN201010584944 A CN 201010584944A CN 201010584944 A CN201010584944 A CN 201010584944A CN 102119594 B CN102119594 B CN 102119594B
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Abstract
The invention discloses a method for promoting rhododendron parvifolium seed germination. Before a seed is sown, the seed is subjected to secondary seed soaking processing. The method comprises the following concrete steps of: (1) soaking the rhododendron parvifolium seed in gibberellin (GA3) for 24 hours, wherein the concentration of GA3 is between 400mg/l and 500mg/l; (2) after cleaning with clear water and absorbing water, soaking the seed in polyethylene glycol 6000 (PEG-6000) for 24 hours, wherein the concentration of the PEG-6000 is 10 percent; and (3) culturing at 25 DEG C or so for 15-20 days to obtain a germinated seed. By using the method, the seed budding rate is improved to 80-83 percent, the germination rate of the rhododendron parvifolium seed is greatly improved, and the germination time is shortened. The invention solves the problem of difficult germination of the rhododendron parvifolium, has the advantages of simple operation and low cost and can be popularized and applied to the introduction and domestication and seedling culture production of the rhododendron parvifolium.
Description
Technical field
The invention belongs to field of plant growing technology, especially woody ornamentais cultivation propagation method field is specifically related to a kind of technical method that promotes the capitate rhododendron branchlet and leaf seed germination.
Background technology
Capitate rhododendron branchlet and leaf (Rhododendron parvifolium) is the evergreen undershrub of Ericaceae Rhododendron; Mainly be distributed in high mountain, tundra, many rocks or the marshland in Daxing'an Mountainrange, China northeast, Changbai Mountain and the Inner Mongol; Belong to vulnerable species, Jilin Province first class of protection plant.Its branch is dense, branch is elongated, the scattered branch of leaf top, keratin.Give birth on the umbel top, has colored 2-6; The wide funnel-form of corolla, rose look to purple rose look.Capsule, oval shape is avette, and is close by scale.The florescence 5-7 month, the fruit phase 9-10 month.
That capitate rhododendron branchlet and leaf gathers is medicinal, fragrant, view and admire, is to be precious from head to foot.Effects such as its leaf has cough-relieving, relieving asthma, eliminate the phlegm have excellent curative to control the elderly's tracheitis and pulmonary emphysema.Leaf and spray are rich in aromatic oil, and wherein leaf volatile oil content is up to 2.7%, and the volatile oil quality is very excellent, and leaf capable of using and spray extract high-quality aromatic oil.Pattern is pleasant, and flower, leaf all has special fragrance, and sight is splendid.Simultaneously, capitate rhododendron branchlet and leaf is cold-resistant, waterlogging, is the important wild excellent germplasm resource of Rhododendron.
Capitate rhododendron branchlet and leaf is of many uses, so DEVELOPMENT PROSPECT is wide.Simultaneously because of nature, many-sided factor such as biological and artificial, reduce day by day the capitate rhododendron branchlet and leaf distributed areas, and the protection work of capitate rhododendron branchlet and leaf is also extremely urgent.The breeding of growing directly from seeds is one of main modes of reproduction of azalea, and researchers from the practice knowledge of azalea seminal propagation to, the seedling that obtains from seed germination begins domestication, make it to adapt to local weather, be a kind of effective introduction and acclimatization means.The capitate rhododendron branchlet and leaf seed germination rate is low, and therefore the mature and plump seed, improves the capitate rhododendron branchlet and leaf seed germination rate and have great importance about 16%.At present, promote that seed germination has methods such as machinery, medicament, report GA is arranged
3, medicament such as polyethylene glycol can promote some plant seed germinations, GA
3Application is also arranged in the seed germination of other wild species of Rhododendron, but on the capitate rhododendron branchlet and leaf seed germination, how to promote its seed germination still to belong to the unknown.
Summary of the invention
The object of the present invention is to provide a kind of germination rate high, sprout time is short, and seedling is healthy and strong, capitate rhododendron branchlet and leaf seed germination method simple to operate.
For realizing the object of the invention, the invention provides following technical scheme:
A kind of method that promotes the capitate rhododendron branchlet and leaf seed germination, its content comprises: adopt the secondary method for soaking seed to handle before the planting seed, concrete steps are following: (1) is first at gibberellin 3 (GA with the capitate rhododendron branchlet and leaf seed
3) middle seed soaking 24hr, GA
3Concentration is between the 400-500mg/l; (2) clear water is used Macrogol 6000 (PEG-6000) seed soaking 24hr after cleaning suck dry moisture again, and PEG-6000 concentration is 10%; Cultivated 15-20 days about (3) 25 ℃.
2, in the present invention: the capitate rhododendron branchlet and leaf seed gathers for the capitate rhododendron branchlet and leaf capsule after the mature and plump seed after fully air-dry, the screening impurity elimination.
3, in the present invention: in said method, need before the seed soaking the capitate rhododendron branchlet and leaf seed disinfection, with the potassium permanganate soaking disinfection 10min of 10% clorox or 0.5%, clear water is cleaned and is blotted; The medicine percentage composition is the quality percentage composition.
4, in the present invention: described GA
3Solution keeps in Dark Place, and the holding time is no more than 1 month.
5, in the present invention: said step (1), (2) herb liquid consumption are fully greater than capitate rhododendron branchlet and leaf seed treatment amount, and abundant stirring and evenly mixing.
6, in the present invention: GA in the said step (1)
3Concentration is 400mg/l, and step (2) PEG-6000 concentration is 10%; GA in the perhaps said step (1)
3Concentration is 500mg/l, and step (2) PEG-6000 concentration is 10%; All need clear water fully to clean behind step (1), (2), blotting paper blots.
7, in the present invention: the seed broadcasting after blotting is in the matrix with certain humidity, and 20-25 ℃ of cultivation cultivated and need be seen light after 15 days.
The present invention through below multinomial comparative trial, preferably sum up the technical parameter of above-mentioned the best:
1, seed soaking single factor experiment: mature and plump, the capitate rhododendron branchlet and leaf seed of sterilizing, 100 of every processing, triplicate: (1) GA
3Solution seed soaking.Use 100,200,300,400,500,600,700 respectively, the solution seed soaking of 800mg/l, seed soaking time is 12,24,48hr, with deionized water seed soaking contrast.The seed of handling fully cleans and blots that to be placed on filter paper be constant temperature culture in the culture dish of germinating bed.Observed and recorded is also calculated germination (germination rate, sprout time, growth of seedling gesture).The result shows that all concentration handle the capitate rhododendron branchlet and leaf seed germination rate that is significantly increased; Warp 400,500,600mg/l GA
3Solution seed soaking 24hr germination rate is higher, and the seedling plant height is significantly higher than contrast.(2) PEG-6000 solution seed soaking.With 5%, 10%, 15%, 20%PEG-6000 solution carries out osmotic adjustment, seed soaking time is 12,24,48hr, the contrast of deionization water level, all the other steps are with (1).The result shows 5%PEG-6000 seed soaking 48hr, and 10%PEG-6000 seed soaking 24hr all can improve the capitate rhododendron branchlet and leaf seed germination rate, and root growth is more healthy and strong.
2, the seed soaking two-factor is tested mutually: on the basis of above-mentioned test 1, GA is set
3400,500, a 600mg/l3 concentration gradient, seed soaking 24hr, PEG-60005% seed soaking 48hr, 10% seed soaking 24hr secondary method for soaking seed carry out complete trial.The result shows: 400mg/l GA
3With the 10%PEG-6000 24hr that soaks seed respectively, 500mg/lGA
3The highest with soak seed the respectively capitate rhododendron branchlet and leaf seed germination rate of these two combined treatment of 24hr of 10%PEG-6000, reach 82%, 83% respectively, the two difference is not remarkable, and the seedling radicle that obtains through the secondary method for soaking seed is sturdy, cotyledon is loose.
3, the selection of seed germination optimum temperature: on test 2 bases, will place 10 ℃, 15 ℃, 20 ℃, 25 ℃, 30 ℃ insulating box to cultivate respectively through the seed soaking seeds treated.The result shows that 20%, 20 ℃, 25 ℃ germination rates of 10 ℃, 15 ℃ following seed germination rate less thaies are higher, and the two difference is not remarkable.
Technical scheme by the invention described above provides can find out that simple to operate, advantages such as cost is low, germination rate is high, seedling stalwartness that the present invention has use method germination rate of the present invention can rise to 80-83%.
Embodiment
Be further understanding summary of the invention of the present invention and effect, the following examples of giving an example now, employed experimental technique among the embodiment is if no specified otherwise is conventional method:
Embodiment 1, capitate rhododendron branchlet and leaf seed germination laboratory cultures method
1, the preparation of seed: pick up from the capsule of the wild plant of capitate rhododendron branchlet and leaf, fully after the drying its cracking or the half-open capsule that splits are pushed aside, with sieve impurity elimination screening, microscopic inspection removes flat grain with seed, and choosing cleanliness is 100%, the healthy seed of full maturation.
2, the sterilization of seed: it is sterilization 10min or 0.5% potassium permanganate (KMnO in 10% clorox (NaClO) solution that dry seed is immersed in concentration
4) 10min in the solution, distilled water flushing 3-4 time, filter paper blots subsequent use.
3, seed soaking is handled: GA
3Solution with join at present, keeps in Dark Place at present.The capitate rhododendron branchlet and leaf seed of sterilizing is placed centrifuge tube, add 400-500mg/lGA earlier
3Solution, liquor capacity is greater than the seed treatment amount, places that centrifugal stirring makes treatment fluid fully mix with seed to contact, pour out seed, distilled water flushing 3-4 time behind the lucifuge seed soaking 24hr on the little centrifuge.Place dry centrifuge tube after filter paper blots again, add 10%PEG-6000 solution, liquor capacity leaves standstill 24hr also greater than the seed treatment amount after centrifugal the stirring.Take out seed behind the 24hr, 3-4 back of distilled water flushing filter paper blots.
4, sowing is sprouted: seed is put into the culture dish that is lined with filter paper of diameter 9cm, and culture fluid is a distilled water, places 25 ℃ of incubators to cultivate.Regularly spray distilled water to keep seed moistening.Seed begins after 7 days to sprout, and sprouting in 15-20 days finishes.Cultivate after 15 days appropriate illumination to prevent seedling excessive growth.By this method, sprout time lag ahead of time, and germination rate can be increased to 83%.
Embodiment 2, capitate rhododendron branchlet and leaf seed germination field cultivation
1,2,3 with embodiment 1.
4, sowing: matrix is that adequately disinfected ratio is 1: 1 peat, a perlite, packs into and tans by the sun in the seedling-cultivating tray after the sterilization, and 1 ‰ carbendazim irrigate subsequent use.To, the secondary seed soaking also clean seed uniform broadcasting of blotting in the dish of cave, gently cover very thin one deck peat after handling.Encase the cave dish to keep humidity, 20-25 ℃ of cultivation with plastic film afterwards.Take off film after 20 days, visible seed has been unearthed and has become to have the healthy and strong seedling of 2 cotyledons.By this method, germination rate can be increased to 80%.
The above; Be merely the preferable embodiment of the present invention; Be not to concrete restriction of the present invention, but protection scope of the present invention is not limited thereto, the technical staff in any skilled field is in the technical scope that the present invention discloses; The variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.
Claims (7)
1. method that promotes the capitate rhododendron branchlet and leaf seed germination is characterized in that: adopt the secondary method for soaking seed to handle before the planting seed, concrete steps are following: (1) with the capitate rhododendron branchlet and leaf seed earlier at gibberellin 3 (GA
3) middle seed soaking 24hr, GA
3Concentration is between the 400-500mg/l; (2) clear water is used Macrogol 6000 (PEG-6000) seed soaking 24hr after cleaning suck dry moisture again, and PEG-6000 concentration is 10%; Cultivated 15-20 days about (3) 25 ℃.
2. the method for promotion capitate rhododendron branchlet and leaf seed germination according to claim 1 is characterized in that: described capitate rhododendron branchlet and leaf seed gathers for the capitate rhododendron branchlet and leaf capsule after the mature and plump seed after fully air-dry, the screening impurity elimination.
3. the method for promotion capitate rhododendron branchlet and leaf seed germination according to claim 1 is characterized in that: in said method, need before the seed soaking the capitate rhododendron branchlet and leaf seed disinfection, with the potassium permanganate soaking disinfection 10min of 10% clorox or 0.5%, clear water is cleaned and is blotted; The medicine percentage composition is the quality percentage composition.
4. the method for promotion capitate rhododendron branchlet and leaf seed germination according to claim 1 is characterized in that: described GA
3Solution keeps in Dark Place, and the holding time is no more than 1 month.
5. the method for promotion capitate rhododendron branchlet and leaf seed germination according to claim 1 is characterized in that: said step (1), (2) herb liquid consumption are fully greater than capitate rhododendron branchlet and leaf seed treatment amount, and abundant stirring and evenly mixing.
6. the method for promotion capitate rhododendron branchlet and leaf seed germination according to claim 1 is characterized in that: GA in the said step (1)
3Concentration is 400mg/l, and step (2) PEG-6000 concentration is 10%; GA in the perhaps said step (1)
3Concentration is 500mg/l, and step (2) PEG-6000 concentration is 10%; All need clear water fully to clean behind step (1), (2), blotting paper blots.
7. the method for promotion capitate rhododendron branchlet and leaf seed germination according to claim 1 is characterized in that: the seed broadcasting after blotting is in the matrix with certain humidity, and 20-25 ℃ of cultivation cultivated and need be seen light after 15 days.
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| CN201010584944A CN102119594B (en) | 2010-12-13 | 2010-12-13 | Method for promoting rhododendron parvifolium seed germination |
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| CN201010584944A CN102119594B (en) | 2010-12-13 | 2010-12-13 | Method for promoting rhododendron parvifolium seed germination |
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| CN102119594B true CN102119594B (en) | 2012-10-03 |
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| CN103299819B (en) * | 2013-07-06 | 2014-07-23 | 云南省农业科学院花卉研究所 | Precise seedling growing method for alpine rhododendrons |
| CN103609226B (en) * | 2013-12-11 | 2014-12-31 | 福建农林大学 | Method for accelerating germination of ormosia microphylla seeds |
| CN104012382A (en) * | 2014-05-30 | 2014-09-03 | 赵冰 | Large-scale seedling culturing technology for rhododendron calophytum Franch |
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| CN104012209B (en) * | 2014-06-26 | 2015-07-01 | 甘肃中医学院 | Method for promoting seeds of meconopsis integrifolia to germinate |
| CN104145563B (en) * | 2014-08-13 | 2016-02-24 | 云南省林业科学院 | A kind of processing method of large tree cuckoo seed |
| CN105850580A (en) * | 2016-04-08 | 2016-08-17 | 石家庄市神州花卉研究所有限公司 | Substrate for culture of wild rhododendron delavayi seed seedlings |
| CN106717251A (en) * | 2016-11-24 | 2017-05-31 | 贵州大学 | A kind of method for promoting oat seed to sprout |
| CN108934925A (en) * | 2018-08-03 | 2018-12-07 | 江苏省农业科学院 | Seedling medium and its preparation method and application containing Chinese medicine slag |
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| CN109997448A (en) * | 2019-05-20 | 2019-07-12 | 湖南粮安科技股份有限公司 | A kind of processing method improving western Seed Vigor of Melon |
| CN110933970A (en) * | 2019-12-13 | 2020-03-31 | 句容市乡土树种研究所 | Method for improving germination rate of hovenia dulcis seeds |
| CN114982415B (en) * | 2022-05-26 | 2024-03-22 | 黑龙江省林业科学研究所 | Method for improving germination rate of alpine azalea and seedling raising method |
| CN115250814B (en) * | 2022-07-12 | 2024-01-23 | 河南牧业经济学院 | Method suitable for germination accelerating and seedling raising of rhododendron in Henan province |
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